CN215560225U - Sample pretreatment device for RT-PCR (reverse transcription-polymerase chain reaction) test of duck reovirus - Google Patents

Sample pretreatment device for RT-PCR (reverse transcription-polymerase chain reaction) test of duck reovirus Download PDF

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Publication number
CN215560225U
CN215560225U CN202122135322.5U CN202122135322U CN215560225U CN 215560225 U CN215560225 U CN 215560225U CN 202122135322 U CN202122135322 U CN 202122135322U CN 215560225 U CN215560225 U CN 215560225U
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CN
China
Prior art keywords
tray
seat
top end
block
bottom end
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Expired - Fee Related
Application number
CN202122135322.5U
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Chinese (zh)
Inventor
朱明恩
李舫
李汝春
提金凤
迟灵芝
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Shandong Vocational Animal Science and Veterinary College
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Shandong Vocational Animal Science and Veterinary College
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Priority to CN202122135322.5U priority Critical patent/CN215560225U/en
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Abstract

The utility model provides a sample pretreatment device for a duck reovirus RT-PCR test, which relates to the technical field of duck reovirus test and comprises the following components: the device comprises a device main body, wherein a placing bin is arranged at the top end of the device main body; the tray is arranged in the placing bin, and a conical groove is formed in the top end of the tray; the lifting ring drives the extrusion rod to move upwards through the connecting rod, so that the extrusion rod is separated from the top end of the telescopic fixture block, the telescopic fixture block shrinks into the movable seat under the action of the telescopic spring after losing extrusion force, the telescopic fixture block is separated from the fixed hole on the mounting seat in the equipment main body, and the movable seat can be separated from the mounting seat in the equipment main body at the moment, so that the separation of the tray and the equipment main body is realized, and the problem of low working efficiency of the pretreatment device can be solved through the plurality of parts.

Description

Sample pretreatment device for RT-PCR (reverse transcription-polymerase chain reaction) test of duck reovirus
Technical Field
The utility model relates to the technical field of duck reovirus detection, in particular to a sample pretreatment device for a duck reovirus RT-PCR test.
Background
The RT-PCR (Reverse Transcription-Polymerase Chain Reaction) is a technology combining Reverse Transcription (RT) of RNA and Polymerase Chain amplification (PCR) of cDNA, and is a common experimental technology for detecting duck reovirus.
In the PCR pretreatment process of a duck reovirus sample, cells of different components in a sample mixed solution need to be separated through centrifugal equipment, and when the existing centrifugal equipment is used, test tubes filled with the sample need to be manually placed into a hole groove on the centrifugal equipment one by an operator, and the test tubes are taken out one by one after the centrifugal operation is finished, so that the working efficiency of the sample pretreatment is low, and the working efficiency of the sample pretreatment is greatly influenced.
SUMMERY OF THE UTILITY MODEL
The utility model provides a sample pretreatment device for a duck reovirus RT-PCR test, which aims to solve the problems in the background art.
The technical scheme of the utility model is realized as follows:
a sample pretreatment device for duck reovirus RT-PCR test includes:
the device comprises a device main body, wherein a placing bin is arranged at the top end of the device main body;
the tray is arranged inside the placing bin, and a conical groove is formed in the top end of the tray;
the sample test tube is positioned in the conical groove and penetrates through the side surface of the inner wall of the conical groove to the inside of the tray;
the connecting column is positioned at the top end of the tray and penetrates through the bottom end of the conical groove to the lower part of the tray;
the connecting seat is positioned at the bottom end of the connecting column and is rotationally connected with the connecting column, and the top end of the connecting seat is fixedly connected with a fixed toothed ring which is rotationally sleeved with the outer wall of the connecting column;
the movable ring block is positioned at the bottom end of the tray and is mutually sleeved with the outer wall of the connecting column;
the locking block is fixedly connected to the bottom end of the movable ring block;
the return spring is positioned in the middle of the movable ring block and the tray and is sleeved with the outer wall of the connecting column;
the lifting mechanism is positioned at the top end of the connecting column, penetrates into the connecting column and is used for performing rotation operation and quick disassembly operation on the tray;
and the connecting mechanism is positioned at the bottom end of the connecting seat and penetrates through the connecting seat to be used for quickly connecting and fixing the tray and the equipment main body.
Preferably, the lifting mechanism comprises a lifting ring, a connecting rod, a piston block and an extrusion rod, the lifting ring is located above the connecting column, the connecting rod is located at the bottom end of the lifting ring and penetrates into the connecting column, the piston block is located inside the connecting column and is fixedly connected with the bottom end of the connecting rod, the extrusion rod is fixedly connected to the bottom end of the piston block, a first compression spring and a second compression spring are respectively arranged at the upper end and the lower end of the piston block inside the connecting column, and the first compression spring and the second compression spring are respectively sleeved with the outer walls of the connecting rod and the extrusion rod.
Preferably, coupling mechanism is including sliding seat, lift post, slip post and gyro wheel, the sliding seat is located the bottom of connecting seat, lift post fixed connection is in sliding seat top central point and puts the department, the slip post is located the sliding seat top and is located the both sides of sliding seat, the top of lift post runs through the connecting seat and extends the inside and No. two compression spring's that run through to the spliced pole bottom laminating, the top of slip post is run through to the top of connecting seat and is connected with the gyro wheel rotation, the inside of sliding seat is provided with two flexible fixture blocks, two flexible fixture block is symmetric distribution and runs through the both sides to the sliding seat respectively with the axis of sliding seat, the inside both ends that are located flexible fixture block of sliding seat are provided with expanding spring.
Preferably, the inside bottom of placing the storehouse is provided with the mount pad, the inside motor that is connected with the mount pad that is provided with of equipment main part, the top of mount pad be provided with sliding seat assorted mounting groove, the inner wall side of mounting groove be provided with flexible fixture block assorted fixed orifices.
Preferably, No. one compression spring's elasticity is greater than No. two compression spring's elasticity, the lifter runs through the lift post and contacts with flexible fixture block to the sliding seat is inside, two flexible fixture block is located the both sides of lifter, just flexible fixture block is close to one side top of lifter and is the arc structure.
Preferably, the inside of tray is provided with the slope hole groove with sample test tube assorted, the bottom of tray be provided with activity ring piece assorted recess, slope hole groove runs through to recess inside.
Preferably, the top of gyro wheel closely laminates with the bottom of activity ring piece, the contact position of activity ring piece and spliced pole is provided with the stopper, the outer wall side of spliced pole be provided with stopper assorted spacing groove, the locking piece is located the side top of fixed ring gear, the thickness of locking piece and the tooth's socket interval phase-match of fixed ring gear.
By adopting the technical scheme, the utility model has the beneficial effects that:
1. the tray can be rotated and operated by pulling the lifting ring, the lifting ring drives the extrusion rod to move upwards through the connecting rod, so that the extrusion rod is separated from the top end of the telescopic fixture block, the telescopic fixture block is contracted into the movable seat under the action of the telescopic spring after losing the extrusion force, the telescopic fixture block is separated from the fixed hole on the mounting seat in the equipment main body, and the movable seat can be separated from the mounting seat in the equipment main body at the moment, so that the tray is separated from the equipment main body, and a plurality of sample test tubes can be synchronously taken out or put in through the matching of the parts, so that the working efficiency of the device is effectively improved;
2. through setting up the sliding seat, can contract to inside the connecting seat when sliding seat and desktop contact, and through the slip post, gyro wheel extrusion activity ring piece rebound, the activity ring piece can drive the separation of locking piece and fixed ring gear, and the activity ring piece can contact and promote its rebound one section distance with the bottom of sample test tube, make the top of sample test tube surpass one section distance of tray, thereby be convenient for the separation of sample test tube and tray, and tray accessible spliced pole uses the connecting seat to rotate as the base, the synchronous rotation of activity ring piece can be convenient for to its gyro wheel, the rotation through the tray can be convenient for taking out and putting into of sample tray, can realize the quick installation and the taking out of sample test tube through the cooperation of a plurality of parts above.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic view of the connection between the tray and the connecting base according to the present invention;
FIG. 3 is a sectional view of the tray and the connecting seat of the present invention;
FIG. 4 is a schematic structural view of the coupling mechanism of the present invention;
FIG. 5 is a cross-sectional view of the connecting column, the movable seat and the lifting column of the present invention;
fig. 6 is a sectional view of the connecting seat and the fixed ring gear of the present invention.
Wherein:
1-a device body; 101-placing a bin; 2-a tray; 201-a tapered recess; 3-sample tube; 4-connecting a column; 5-a connecting seat; 501. a fixed gear ring; 6-a lifting mechanism; 601. lifting the ring; 602. a connecting rod; 603. a piston block; 604. an extrusion stem; 605. a compression spring; 606. a second compression spring; 7-a connection mechanism; 701. a movable seat; 702. a lifting column; 703. a sliding post; 704. a roller; 705. a telescopic clamping block; 706. a tension spring; 8-a movable ring block; 9-a locking block; 10-a return spring.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
Examples
Referring to fig. 1-6, the sample pre-treatment device for the duck reovirus RT-PCR test comprises:
the device comprises a device body 1, wherein a placing bin 101 is arranged at the top end of the device body 1;
the tray 2 is arranged in the placing bin 101, and a conical groove 201 is formed in the top end of the tray 2;
the sample tube 3 is positioned in the conical groove 201, and penetrates through the inner wall side surface of the conical groove 201 to the inner part of the tray 2;
the connecting column 4 is positioned at the top end of the tray 2 and penetrates through the bottom end of the conical groove 201 to the lower part of the tray 2;
the connecting seat 5 is positioned at the bottom end of the connecting column 4 and is rotationally connected with the connecting column 4, and the top end of the connecting seat 5 is fixedly connected with a fixed toothed ring 501 which is rotationally sleeved with the outer wall of the connecting column 4;
the movable ring block 8 is positioned at the bottom end of the tray 2 and is sleeved with the outer wall of the connecting column 4;
the locking block 9, the locking block 9 is fixedly connected to the bottom end of the movable ring block 8;
the return spring 10 is positioned in the middle of the movable ring block 8 and the tray 2, and the return spring 10 is sleeved with the outer wall of the connecting column 4;
the lifting mechanism 6 is positioned at the top end of the connecting column 4, penetrates into the connecting column 4 and is used for performing rotation operation and quick disassembly operation on the tray 2;
and the connecting mechanism 7 is positioned at the bottom end of the connecting seat 5 and penetrates into the connecting seat 5, and is used for quickly connecting and fixing the tray 2 and the equipment main body 1.
Wherein, the lifting mechanism 6 includes a lifting ring 601, a connecting rod 602, a piston block 603 and an extrusion rod 604, the lifting ring 601 is located above the connecting column 4, the connecting rod 602 is located at the bottom end of the lifting ring 601 and runs through to the inside of the connecting column 4, the piston block 603 is located inside the connecting column 4 and is fixedly connected with the bottom end of the connecting rod 602, the extrusion rod 604 is fixedly connected at the bottom end of the piston block 603, the upper and lower ends of the inside of the connecting column 4, which is located in the piston block 603, are respectively provided with a compression spring 605 and a compression spring 606, the first compression spring 605 and the second compression spring 606 are respectively connected with the connecting rod 602, the outer wall of the extrusion rod 604 is mutually sleeved, the convenient disassembly operation of the tray 2 and the device main body 1 can be carried out through the lifting mechanism 6, thereby the synchronous taking out of the plurality of sample test tubes 3 is facilitated.
Wherein, coupling mechanism 7 is including sliding seat 701, lift post 702, sliding column 703 and gyro wheel 704, sliding seat 701 is located the bottom of connecting seat 5, lift post 702 fixed connection is in sliding seat 701 top central point department of putting, sliding column 703 is located the top of sliding seat 701 and is located the both sides of sliding seat 701, the top of lift post 702 runs through connecting seat 5 and extends to run through to the inside of spliced pole 4 and the laminating of the bottom of No. two compression spring 606, the top of sliding column 703 runs through to the top of connecting seat 5 and is connected with gyro wheel 704 rotates, the inside of sliding seat 701 is provided with two flexible fixture blocks 705, two flexible fixture blocks 705 are the symmetric distribution and run through to the both sides of sliding seat 701 respectively with the axis of sliding seat 701, the inside both ends that are located flexible fixture block 705 of sliding seat 701 are provided with expanding spring 706, can realize the quick installation of tray 2 through coupling mechanism 7.
Wherein, the inside bottom of placing storehouse 101 is provided with the mount pad, and equipment main part 1 is inside to be provided with the motor of being connected with the mount pad, and the top of mount pad is provided with the mounting groove with sliding seat 701 assorted, and the inner wall side of mounting groove is provided with the fixed orifices with flexible fixture block 705 assorted, can realize tray 2's transmission operation through the cooperation of equipment main part 1 inside motor and mount pad to realize the centrifugal separation of sample.
The elastic force of the first compression spring 605 is greater than that of the second compression spring 606, the extrusion rod 604 penetrates through the lifting column 702 to be in contact with the telescopic clamping blocks 705 inside the movable seat 701, the two telescopic clamping blocks 705 are located on two sides of the extrusion rod 604, the top end of one side, close to the extrusion rod 604, of each telescopic clamping block 705 is of an arc-shaped structure, and the extrusion rod 604 can always keep extruding the telescopic clamping blocks 705 through the matching of the first compression spring 605 and the second telescopic clamping block 606.
Wherein, tray 2's inside be provided with sample test tube 3 assorted slope hole groove, tray 2's bottom be provided with 8 assorted recesses of activity ring piece, slope hole groove runs through to the recess inside, can make activity ring piece 8 can the rebound and promote sample test tube 3 rebound through this structure to make sample test tube 3 take out the operation.
Wherein, the top of gyro wheel 704 closely laminates with the bottom of activity ring piece 8, the contact position of activity ring piece 8 and spliced pole 4 is provided with the stopper, spliced pole 4's outer wall side be provided with stopper assorted spacing groove, locking piece 9 is located fixed ring gear 501's side top, the thickness of locking piece 9 and fixed ring gear 501's tooth's socket interval phase-match, can make tray 2 rotate after taking off from equipment main part 1 through this structure, and tray 2 installs the unable rotation behind 1 inside to equipment main part, can only be driven by the inside motor of equipment main part 1 and rotate.
The working principle is as follows: when the sample pretreatment device for the duck reovirus RT-PCR test is used, the rotation of the tray 2 can be realized through the matching of the motor and the mounting seat in the device main body 1, so that the centrifugal operation of a sample on the tray 2 is realized, after the centrifugal operation is finished, the cover on the device main body 1 can be firstly opened, then the lifting ring 601 is upwards pulled, the lifting ring 601 drives the extrusion rod 604 to upwards move through the connecting rod 602, the extrusion rod 604 is separated from the top end of the telescopic fixture block 705, at the moment, the telescopic fixture block 705 loses the extrusion force, and is contracted into the movable seat 701 under the action of the telescopic spring 706, the telescopic fixture block 705 is separated from the fixed hole on the mounting seat in the device main body 1, at the moment, the movable seat 701 can be separated from the mounting seat in the device main body 1, so that the separation of the tray 2 and the device main body 1 is realized, then the taken-down tray 2 is placed on a desktop, the movable seat 701 can contact with the table top and be forced to move towards the direction of the connecting seat 5, the movable seat 701 can contract into the connecting seat 5, meanwhile, the sliding column 703 can be pushed to move upwards, the movable ring block 8 is extruded to move upwards through the roller 704, the movable ring block 8 can drive the locking block 9 to separate from the fixed gear ring 501, the movable ring block 8 can contact with the bottom end of the sample test tube 3 and push the sample test tube 3 to move upwards for a certain distance, so that the top end of the sample test tube 3 exceeds the tray 2 for a certain distance, thereby facilitating the separation of the sample test tube 3 and the tray 2, when the sample test tube 3 is taken off from the tray 2, the tray 2 can rotate through the connecting column 4 by taking the connecting seat 5 as a base, the roller 704 can facilitate the synchronous rotation of the movable ring block 8, the taking-out and putting-in of the sample tray 2 can be facilitated through the rotation of the tray 2, after the tray 2 on the device main body 1 is taken out, the tray 2 with the sample test tube 3 placed outside can be synchronously installed inside the device main body 1 and is transferred through the lifting ring 601, when the movable seat 701 is separated from the desktop, the movable seat 701 can move downwards under the action of self gravity and the second compression spring 606, the roller 704 and the movable ring block 8 can synchronously move downwards, the locking block 9 at the bottom end of the movable ring block 8 can be clamped with the tooth groove of the fixed tooth ring 501, so that the tray 2 cannot rotate at the moment, the sample test tube 3 is completely sleeved on the tray 2, then the movable seat 701 is aligned with the installation groove on the installation seat inside the device main body 1 to be put in, after the lifting ring 601 is released, the piston block 603 and the extrusion rod 604 can move downwards under the action of the first compression spring 605, the extrusion rod can be contacted with the telescopic clamping block 705 and can extrude the telescopic clamping block 705 to move, the telescopic clamping block 705 moves to the outside of the movable seat 7601 and is clamped with the fixed hole inside the installation groove, thereby realize fixed mounting, can effectively improve the work efficiency of equipment through the cooperation of above a plurality of parts.
The present invention is not limited to the above-described preferred embodiments, but rather, the present invention is to be construed broadly and cover all modifications, equivalents, and improvements falling within the spirit and scope of the present invention.

Claims (7)

1. A sample pretreatment device for duck reovirus RT-PCR test, its characterized in that includes:
the device comprises a device main body (1), wherein a placing bin (101) is arranged at the top end of the device main body (1);
the tray (2) is arranged inside the placing bin (101), and a conical groove (201) is formed in the top end of the tray (2);
the sample test tube (3) is positioned inside the conical groove (201) and penetrates through the side surface of the inner wall of the conical groove (201) to the inside of the tray (2);
the connecting column (4) is positioned at the top end of the tray (2) and penetrates through the bottom end of the conical groove (201) to the position below the tray (2);
the connecting device comprises a connecting seat (5), wherein the connecting seat (5) is positioned at the bottom end of a connecting column (4) and is rotatably connected with the connecting column (4), and the top end of the connecting seat (5) is fixedly connected with a fixed toothed ring (501) which is rotatably sleeved with the outer wall of the connecting column (4);
the movable ring block (8) is positioned at the bottom end of the tray (2) and is sleeved with the outer wall of the connecting column (4);
the locking block (9), the said locking block (9) is fixedly connected to the bottom end of the movable ring block (8);
the reset spring (10) is positioned in the middle of the movable ring block (8) and the tray (2) and is sleeved with the outer wall of the connecting column (4);
the lifting mechanism (6) is positioned at the top end of the connecting column (4), penetrates into the connecting column (4), and is used for performing rotation operation and quick disassembly operation on the tray (2);
the connecting mechanism (7) is located at the bottom end of the connecting seat (5) and penetrates into the connecting seat (5) to be used for quickly connecting and fixing the tray (2) and the equipment main body (1).
2. The sample pretreatment device for the RT-PCR test of the duck reovirus according to claim 1, wherein the lifting mechanism (6) comprises a lifting ring (601), a connecting rod (602), a piston block (603) and an extrusion rod (604), the lifting ring (601) is located above the connecting column (4), the connecting rod (602) is located at the bottom end of the lifting ring (601) and penetrates into the connecting column (4), the piston block (603) is located inside the connecting column (4) and is fixedly connected with the bottom end of the connecting rod (602), the extrusion rod (604) is fixedly connected with the bottom end of the piston block (603), a first compression spring (605) and a second compression spring (606) are respectively arranged at the upper end and the lower end of the piston block (603) in the connecting column (4), and the first compression spring (605) and the second compression spring (606) are respectively connected with the connecting rod (602), The outer walls of the extrusion rods (604) are sleeved with each other.
3. The sample pretreatment device for the RT-PCR test of the duck reovirus as claimed in claim 2, wherein the connection mechanism (7) comprises a movable seat (701), a lifting column (702), sliding columns (703) and rollers (704), the movable seat (701) is located at the bottom end of the connection seat (5), the lifting column (702) is fixedly connected at the center of the top end of the movable seat (701), the sliding columns (703) are located at the top end of the movable seat (701) and located at two sides of the movable seat (701), the top end of the lifting column (702) penetrates through the connection seat (5) and extends to penetrate through the inside of the connection column (4) to be attached to the bottom end of the second compression spring (606), the top end of the sliding column (703) penetrates above the connection seat (5) to be rotatably connected with the rollers (704), two telescopic fixture blocks (705) are arranged inside the movable seat (701), the two telescopic fixture blocks (705) are symmetrically distributed around the central axis of the movable seat (701) and respectively penetrate through the two sides of the movable seat (701), and telescopic springs (706) are arranged at the two ends, located on the telescopic fixture blocks (705), in the movable seat (701).
4. The sample pretreatment device for the RT-PCR test of the duck reovirus as claimed in claim 3, wherein a mounting seat is arranged at the bottom end of the interior of the placing bin (101), a motor connected with the mounting seat is arranged in the device main body (1), an installation groove matched with the movable seat (701) is arranged at the top end of the mounting seat, and a fixing hole matched with the telescopic fixture block (705) is arranged on the side surface of the inner wall of the installation groove.
5. The sample pretreatment device for the RT-PCR test of the duck reovirus as claimed in claim 3, wherein the elastic force of the first compression spring (605) is greater than the elastic force of the second compression spring (606), the extrusion rod (604) penetrates through the lifting column (702) to the inside of the movable seat (701) to be in contact with the telescopic fixture blocks (705), the two telescopic fixture blocks (705) are located on two sides of the extrusion rod (604), and the top end of one side, close to the extrusion rod (604), of each telescopic fixture block (705) is in an arc structure.
6. The device for pretreating samples of duck reovirus RT-PCR (reverse transcription-polymerase chain reaction) tests as claimed in claim 1, wherein the tray (2) is internally provided with inclined hole slots matched with the sample test tubes (3), the bottom end of the tray (2) is provided with grooves matched with the movable ring blocks (8), and the inclined hole slots penetrate into the grooves.
7. The sample pretreatment device for the RT-PCR test of the duck reovirus as claimed in claim 3, wherein the top end of the roller (704) is closely attached to the bottom end of the movable ring block (8), the contact position of the movable ring block (8) and the connecting column (4) is provided with a limiting block, the side surface of the outer wall of the connecting column (4) is provided with a limiting groove matched with the limiting block, the locking block (9) is located above the side surface of the fixed toothed ring (501), and the thickness of the locking block (9) is matched with the tooth space interval of the fixed toothed ring (501).
CN202122135322.5U 2021-09-06 2021-09-06 Sample pretreatment device for RT-PCR (reverse transcription-polymerase chain reaction) test of duck reovirus Expired - Fee Related CN215560225U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202122135322.5U CN215560225U (en) 2021-09-06 2021-09-06 Sample pretreatment device for RT-PCR (reverse transcription-polymerase chain reaction) test of duck reovirus

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202122135322.5U CN215560225U (en) 2021-09-06 2021-09-06 Sample pretreatment device for RT-PCR (reverse transcription-polymerase chain reaction) test of duck reovirus

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CN215560225U true CN215560225U (en) 2022-01-18

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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114801114A (en) * 2022-05-12 2022-07-29 贵州富强科技包装有限公司 Anti-bridging material barrel of extrusion machine

Cited By (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN114801114A (en) * 2022-05-12 2022-07-29 贵州富强科技包装有限公司 Anti-bridging material barrel of extrusion machine
CN114801114B (en) * 2022-05-12 2024-09-20 贵州富强科技包装有限公司 Anti-bridging charging barrel of extrusion machine

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Granted publication date: 20220118