CN215530993U - Indoor population culture device for trichlorfon - Google Patents

Abstract

The utility model discloses a device for culturing indoor population of trichlorfon, which comprises a culture cylinder; the culture tank is detachably and horizontally arranged in the culture tank and comprises a bottom plate and a frame, and the frame is detachably spliced with the plurality of plate dividing bodies of the bottom plate; the plurality of U-shaped slots are formed in the plurality of the plate dividing bodies, two U-shaped slots at the splicing positions of two adjacent plate dividing bodies are arranged in parallel and close to each other, and two ends of each U-shaped slot extend to the inner side wall of the frame vertically connected with the bottom plate; a plurality of baffles, one of which is correspondingly and detachably matched with a plurality of U-shaped slots; an oxygenation assembly and a temperature sensor disposed within the incubation cylinder; and an incandescent lamp tube disposed at the top of the culture tank. The indoor population culture device for the trichinella parvus, provided by the utility model, can be used for conveniently and rapidly replacing one or more sub-plates and sediments or culture media paved on the sub-plates at regular intervals, is convenient and fast to operate, and effectively establishes the indoor population of the trichinella parvus on the basis of obtaining high survival rate.

Description

Indoor population culture device for trichlorfon

Technical Field

The utility model relates to the technical field of fertilized egg incubation, in particular to a device for culturing indoor population of small head worms.

Background

The small head worm (Capitella teleta) belongs to the Polychaeta of Annelida, has simple morphological structure, cylindrical worm body (similar to earthworm) and red body length ranging from several mm to 40mm in life. The organic pollution indicator is an organic pollution indicator widely distributed in the world sea area, is also a good toxicology experimental material, and can be applied to the ecological restoration of organic pollution in the offshore area. Therefore, there is a need to establish indoor population of the trichuris parvum to deeply study morphological and life history characteristics of the trichuris parvum population, so as to lay a foundation for deep study on systematic classification and population genetic differentiation.

The existing device for culturing the trichlorfon in the laboratory is not complete enough, generally adopts a fish tank to bear the deposit with the trichlorfon for culturing, is inconvenient to replace the deposit, is not easy to keep the water quality requirement, and can not meet the experimental requirement of establishing the trichlorfon population in the laboratory.

SUMMERY OF THE UTILITY MODEL

An object of the present invention is to solve at least the above problems and to provide at least the advantages described later.

Still another object of the present invention is to provide a device for cultivating indoor population of trichinella, which can be used to conveniently and rapidly replace one or more plates and the sediments or culture mediums laid thereon at regular intervals, and has the advantages of simple structure, convenient operation, and effective establishment of indoor population of trichinella on the basis of high survival rate.

To achieve these objects and other advantages in accordance with the purpose of the utility model, there is provided an indoor population culture device of a small head worm, comprising:

cultivating in a jar;

the culture tank is detachably and horizontally arranged in the culture tank, and comprises a bottom plate which is of a plate body structure, and the bottom plate comprises a plurality of split plate bodies which are spliced; the frame is vertically formed on the edge of the bottom plate and is detachably spliced with the plurality of the sub-plate bodies; the plurality of U-shaped slots are formed in the plurality of the plate dividing bodies, one U-shaped slot is formed in each of the two plate dividing bodies positioned at the two ends of the culture tank, two U-shaped slots are formed in the other plate dividing bodies positioned in the middle of the culture tank, the two U-shaped slots at the splicing positions of the two adjacent plate dividing bodies are arranged in a close manner in parallel, and the two ends of each U-shaped slot extend to the inner side wall of the frame vertically connected with the bottom plate; a plurality of baffles, one of which is correspondingly and detachably matched with a plurality of U-shaped slots; an oxygenation assembly and a temperature sensor disposed within the incubation cylinder; and an incandescent lamp tube disposed at the top of the culture tank.

Preferably, the height of the frame is less than 5cm, and two ends of the plurality of U-shaped slots extend upward and protrude from the upper edge of the frame relatively.

Preferably, the culture tank further comprises: the supporting plate is horizontally arranged in the culture cylinder, three adjacent edges of the supporting plate are hermetically fixed on the inner side wall of the culture cylinder so as to divide the culture cylinder into a culture cavity and a backwater buffer cavity which are distributed up and down, and the edge of the remaining end of the supporting plate is spaced from the inner side wall of the culture cylinder by a certain distance so as to form a waterway channel communicated between the culture cavity and the backwater buffer cavity;

the first baffle plate and the second baffle plate are correspondingly and transversely arranged on the supporting plate, and two ends of the first baffle plate and the second baffle plate are hermetically fixed with the inner side wall of the culture cylinder so as to form an accommodating groove between the first baffle plate and the second baffle plate; the first baffle plate is arranged close to the waterway channel at one end of the support plate, the second baffle plate is arranged close to the other end of the support plate, and a water replenishing groove is formed between the outer side of the second baffle plate and the culture cylinder as well as the support plate; the culture tank is detachably arranged in the accommodating tank in an adaptive mode, the bottom of the culture tank is flatly laid on the upper surface of the supporting plate, and the heights of the first baffle plate and the second baffle plate are both larger than the height of a frame of the culture tank; the first through holes are uniformly arranged on the first baffle plate in a penetrating way at intervals, the arrangement height of the first through holes is higher than the frame height of the culture tank, and the vertical height of the first through holes relative to the bottom plate is 7-9 cm; the second through holes are uniformly spaced on the second baffle plate, the height of the second through holes is higher than that of the frame of the culture tank, and the vertical height of the second through holes relative to the bottom plate is 6-8 cm; and

and the pump is arranged in the water return buffer cavity, and a water outlet pipe of the pump extends into the water supplementing groove.

Preferably, the method further comprises the following steps: the heating rod is arranged in the water return buffer cavity; and the oxygenation assembly II is arranged in the water return buffer cavity.

Preferably, the method further comprises the following steps: a plurality of handles or a plurality of couple, its even dispersion is opened vertical setting on the frame of culture tank, and corresponds at least on arbitrary plate partitioning body and set up two handles or two couples.

Preferably, the method further comprises the following steps: and the filter screen is arranged on the plurality of first through holes.

Preferably, the specification of the bottom plate is 50-60cm in length and 30-40cm in width;

the vertical distance between the bottom plate and the upper opening of the culture tank is 30-40 cm.

The utility model at least comprises the following beneficial effects:

the indoor population culture device for the trichinella parvus, provided by the utility model, can be used for conveniently and rapidly replacing one or more sub-plates and sediments or culture media paved on the sub-plates at regular intervals, is simple in structure and convenient and fast to operate, and effectively establishes the indoor population of the trichinella parvus on the basis of obtaining high survival rate.

Additional advantages, objects, and features of the utility model will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the utility model.

Drawings

FIG. 1 is a schematic front view of an apparatus for cultivating an indoor population of a trichina according to an embodiment of the present invention;

FIG. 2 is a schematic top view of the culture tank according to an embodiment of the present invention;

FIG. 3 is a schematic side view of a culture tank according to still another embodiment of the present invention;

FIG. 4 is a schematic front view of an apparatus for cultivating an indoor population of a small head insect according to still another embodiment of the present invention;

FIG. 5 is a schematic side view showing a structure of a culture tank according to still another embodiment of the present invention.

Detailed Description

The present invention is further described in detail below with reference to the attached drawings so that those skilled in the art can implement the utility model by referring to the description text.

It will be understood that terms such as "having," "including," and "comprising," as used herein, do not preclude the presence or addition of one or more other elements or groups thereof.

As shown in FIGS. 1 to 2, the present invention provides an indoor population culture device for a trichina, comprising:

a culture cylinder 10; the culture tank is made of glass generally, so that the observation is convenient, and the thickness of the glass is 3mm, 4mm or 5 mm; local materials can be used, and a cuboid glass fish tank is selected for reconstruction, so that the cost is saved, and convenience and rapidness are realized; the culture tank is detachably and horizontally arranged in the culture tank, the culture tank 20 comprises a bottom plate 201 which is of a plate body structure, and the bottom plate comprises a plurality of split plate bodies which are spliced; the edges of the splicing positions among the plurality of the plate dividing bodies are smoothly processed so as to be convenient for randomly taking out one of the plate dividing bodies; without hindering the free migration of the nauplius in the sediment on the plurality of sub-plates in the culture tank; the frame 202 is vertically formed on the edge of the bottom plate, and the frame is detachably spliced with the plurality of the sub-plate bodies; the plurality of U-shaped slots 203 are formed in the plurality of the plate dividing bodies, one U-shaped slot is arranged on each of the two plate dividing bodies positioned at the two ends of the culture tank, two U-shaped slots are arranged on the other plate dividing bodies positioned in the middle of the culture tank, the two U-shaped slots at the splicing position of the two adjacent plate dividing bodies are arranged in a mutually parallel and adjacent mode, and the two ends of the plurality of U-shaped slots extend to the inner side wall of the frame vertically connected with the bottom plate; a plurality of baffles 204, one of which is correspondingly and detachably matched with a plurality of U-shaped slots; an oxygenation assembly 30 and a temperature sensor 40 disposed within the culture tank; and an incandescent lamp 50 disposed at the top of the culture pot. In the scheme, a culture tank and an culture cylinder are arranged in a structure matched mode, sediment or culture medium with microcephalus worms with a certain thickness is laid in the culture tank, artificial seawater with the salinity of 25 per thousand is added, water is 3-4cm higher than the sediment or culture medium, oxygenation culture is carried out, an incandescent lamp provides illumination, the light and shade time is set to a certain proportion, and the temperature of a culture chamber (detected by a temperature sensor) where the culture cylinder is located is kept at 20-25 ℃; in the culture process, 500ml of fresh water fully aerated is added into the culture tank every other week to supplement the evaporated water. And digging out 1/3, 1/4 or 1/5 volumes of sediments in the culture tank every 25-35 days, and filling a new culture medium in situ to provide a new inhabitation matrix and food for the trichuris. The operation of replacing the sediments or the culture medium seems simple, but if the sediments or the culture medium are directly dug from the bottom of the culture cylinder, the water in the culture cylinder can be stirred, the sediments to be replaced are not easy to be dug out completely, and the uniform and new culture medium is not easy to be laid; in the utility model, the culture tank is formed by splicing a plurality of the sub-plate bodies, the sediment or the culture medium is laid on the bottom plate, when the culture tank needs to be replaced, the plurality of baffles can be correspondingly inserted into the plurality of U-shaped slots for blocking, and then one or two of the sub-plate bodies together with the sediment or the culture medium thereon can be directly pulled out for replacement, so that the rapid replacement is realized, and the water body cannot be disturbed excessively. On the basis of obtaining high survival rate, the indoor population of the trichlorfon can be effectively established in a short period.

As shown in fig. 3, in a preferred embodiment, the height of the frame is less than 5cm (3cm or 4cm, etc.), the frame has a reasonable height for accommodating sediments and preventing the water from disturbing the frame too much, and two ends of the plurality of U-shaped slots extend upward and protrude from the upper edge of the frame relatively. In this scheme, the both ends of a plurality of U-shaped slots upwards extend the setting, can make it extend the water take the altitude according to actual need to in order that the laboratory technician sees fast and insert the baffle wherein fast, if protrusion in water, also be favorable to the laboratory technician gripping so that mention the branch plate body that corresponds, simple structure, convenient operation.

As shown in FIG. 4, in a preferred embodiment, the culture tank further comprises: the supporting plate 101 is horizontally arranged in the culture cylinder, three adjacent edges of the supporting plate are hermetically fixed on the inner side wall of the culture cylinder so as to divide the culture cylinder into a culture cavity and a backwater buffer cavity which are distributed up and down, and the edge of the remaining end of the supporting plate is spaced from the inner side wall of the culture cylinder by a certain distance so as to form a waterway channel 102 communicated between the culture cavity and the backwater buffer cavity; the first baffle plate 103 and the second baffle plate 104 are correspondingly and transversely arranged on the support plate, and two ends of the first baffle plate and the second baffle plate are hermetically fixed with the inner side wall of the culture cylinder so as to form an accommodating groove between the first baffle plate and the second baffle plate; the first baffle plate is arranged close to the waterway channel at one end of the support plate, the second baffle plate is arranged close to the other end of the support plate, and a water replenishing groove 105 is formed between the outer side of the second baffle plate and the culture cylinder as well as the support plate; the culture tank is detachably arranged in the accommodating tank in an adaptive mode, the bottom of the culture tank is flatly laid on the upper surface of the supporting plate, and the heights of the first baffle plate and the second baffle plate are both larger than the height of a frame of the culture tank; the first through holes are uniformly arranged on the first baffle plate in a penetrating way at intervals, the arrangement height of the first through holes is higher than the frame height of the culture tank, and the vertical height of the first through holes relative to the bottom plate is 7-9cm (7cm, 8cm or 9cm and the like); during cultivation, the water level above the culture tank is kept to be 3-4cm (3cm or 4cm and the like), and the positions of the first through holes are flush with the water level above the culture tank, so that the first through holes not only play a role of circulating water, but also play a role of keeping the water level; the second through holes are uniformly spaced on the second baffle plate, the setting height of the second through holes is higher than the frame height of the culture tank, and the vertical height of the second through holes relative to the bottom plate is 6-8cm (6cm, 7cm or 8cm and the like); and the pump 106 is arranged in the water return buffer cavity, and a water outlet pipe of the pump extends into the water supplementing groove. In the scheme, the water channel is communicated between the culture cavity and the backwater buffer cavity, and then water circularly flows among the backwater buffer cavity, the water supplementing groove, the culture groove and the water channel under the pumping of the pump, so that a proper running water environment is provided for the small head insects in the culture groove; the apertures of the first through holes and the second through holes are small, so that water can slowly flow into the culture tank through the second through holes and then flow out of the culture tank through the first through holes, sediment or culture medium cannot be disturbed due to too high flow speed, and excessive sediment or culture medium is prevented from flowing out of the culture tank along with flowing water.

In a preferred embodiment, the method further comprises: the heating rod is arranged in the water return buffer cavity; and the oxygenation assembly II is arranged in the water return buffer cavity. In the scheme, the heating rod is used for heating the water body appropriately, so that the temperature of the water body is kept at 18-23 ℃ (18 ℃, 20 ℃, 23 ℃ or the like); the oxygenation component II is used for aeration and oxygenation and can also promote the water body in the backwater buffer cavity to be heated uniformly.

As shown in fig. 5, in a preferred embodiment, the method further includes: a plurality of handles or a plurality of couple 205, its even dispersion is vertical to be set up on the frame of culture tank, and corresponds at least on arbitrary plate body and sets up two handles or two couples. In this scheme, a plurality of handles or a plurality of couple are used for the gripping, can realize convenient dismouting culture tank and change deposit or culture medium.

In a preferred embodiment, the method further comprises: and the filter screen is arranged on the plurality of first through holes. In the scheme, in order to prevent the larva of the small head worm (with the length from 100 microns to 600 microns) swimming in the water from swimming out of the culture tank, the filter screens are arranged on the first through holes and are prevented from entering the backwater buffer cavity; the artificial seawater in the partial backwater buffer cavity can be replaced regularly. For example, once every 25-35 days.

In a preferable scheme, the specification of the bottom plate is 50-60cm (50cm, 55cm or 60cm and the like) in length and 30-40cm (30cm, 35cm or 40cm and the like) in width; in practical application, the bottom plate can comprise 3 plate dividing bodies, 4 plate dividing bodies or 5 plate dividing bodies which are sequentially connected and arranged; the vertical distance between the bottom plate and the upper opening of the culture tank is 30-40cm (30cm, 33cm, 36cm or 40cm, etc.). The side wall of the culture cylinder is higher than the bottom plate by a certain height, so that on one hand, the artificial seawater in aeration can be prevented from splashing to the test bed around the culture cylinder, and the test bed is prevented from being corroded; on the other hand, the artificial seawater in the aeration can be prevented from splashing to other culture pots arranged around the culture pot, and the water body of the adjacent culture pot is prevented from being polluted.

While embodiments of the utility model have been described above, it is not limited to the applications set forth in the description and the embodiments, which are fully applicable in various fields of endeavor to which the utility model pertains, and further modifications may readily be made by those skilled in the art, it being understood that the utility model is not limited to the details shown and described herein without departing from the general concept defined by the appended claims and their equivalents.

Claims (7)

1. An indoor population culture device of small headerficial worm, characterized by includes:

cultivating in a jar;

the culture tank is detachably and horizontally arranged in the culture tank, and comprises a bottom plate which is of a plate body structure, and the bottom plate comprises a plurality of split plate bodies which are spliced; the frame is vertically formed on the edge of the bottom plate and is detachably spliced with the plurality of the sub-plate bodies; the plurality of U-shaped slots are formed in the plurality of the plate dividing bodies, one U-shaped slot is formed in each of the two plate dividing bodies positioned at the two ends of the culture tank, two U-shaped slots are formed in the other plate dividing bodies positioned in the middle of the culture tank, the two U-shaped slots at the splicing positions of the two adjacent plate dividing bodies are arranged in a close manner in parallel, and the two ends of each U-shaped slot extend to the inner side wall of the frame vertically connected with the bottom plate; a plurality of baffles, one of which is correspondingly and detachably matched with a plurality of U-shaped slots;

an oxygenation assembly and a temperature sensor disposed within the incubation cylinder; and

and the incandescent lamp tube is arranged at the top of the culture tank.

2. The device for cultivating indoor population of trichlorfon as claimed in claim 1, wherein the height of the frame is less than 5cm, and two ends of the plurality of U-shaped slots extend upwards and protrude from the upper edge of the frame.

3. The device for cultivating indoor population of small head insects as claimed in claim 1, wherein said culture tank further comprises:

the supporting plate is horizontally arranged in the culture cylinder, three adjacent edges of the supporting plate are hermetically fixed on the inner side wall of the culture cylinder so as to divide the culture cylinder into a culture cavity and a backwater buffer cavity which are distributed up and down, and the edge of the remaining end of the supporting plate is spaced from the inner side wall of the culture cylinder by a certain distance so as to form a waterway channel communicated between the culture cavity and the backwater buffer cavity;

the first baffle plate and the second baffle plate are correspondingly and transversely arranged on the supporting plate, and two ends of the first baffle plate and the second baffle plate are hermetically fixed with the inner side wall of the culture cylinder so as to form an accommodating groove between the first baffle plate and the second baffle plate; the first baffle plate is arranged close to the waterway channel at one end of the support plate, the second baffle plate is arranged close to the other end of the support plate, and a water replenishing groove is formed between the outer side of the second baffle plate and the culture cylinder as well as the support plate; the culture tank is detachably arranged in the accommodating tank in an adaptive mode, the bottom of the culture tank is flatly laid on the upper surface of the supporting plate, and the heights of the first baffle plate and the second baffle plate are both larger than the height of a frame of the culture tank; the first through holes are uniformly arranged on the first baffle plate in a penetrating way at intervals, the arrangement height of the first through holes is higher than the frame height of the culture tank, and the vertical height of the first through holes relative to the bottom plate is 7-9 cm; the second through holes are uniformly spaced on the second baffle plate, the height of the second through holes is higher than that of the frame of the culture tank, and the vertical height of the second through holes relative to the bottom plate is 6-8 cm; and

and the pump is arranged in the water return buffer cavity, and a water outlet pipe of the pump extends into the water supplementing groove.

4. The device for cultivating indoor population of small headworms of claim 3, further comprising:

the heating rod is arranged in the water return buffer cavity; and

and the oxygenation assembly II is arranged in the water return buffer cavity.

5. The device for cultivating an indoor population of a trichlorfon as claimed in claim 1, further comprising:

a plurality of handles or a plurality of couple, its even dispersion is opened vertical setting on the frame of culture tank, and corresponds at least on arbitrary plate partitioning body and set up two handles or two couples.

6. The device for cultivating indoor population of small headworms of claim 3, further comprising:

and the filter screen is arranged on the plurality of first through holes.

7. The device for cultivating indoor population of trichlorfon as claimed in claim 1, wherein the bottom plate has a length of 50-60cm and a width of 30-40 cm;

the vertical distance between the bottom plate and the upper opening of the culture tank is 30-40 cm.

Images