CN215506825U - Centrifugal micro-fluidic chip for instant mixing and detection - Google Patents
Centrifugal micro-fluidic chip for instant mixing and detection Download PDFInfo
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- CN215506825U CN215506825U CN202121234872.6U CN202121234872U CN215506825U CN 215506825 U CN215506825 U CN 215506825U CN 202121234872 U CN202121234872 U CN 202121234872U CN 215506825 U CN215506825 U CN 215506825U
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Abstract
The utility model discloses a centrifugal micro-fluidic chip for instant mixing and detection.A chip body is provided with a plurality of mixing detection units, and each mixing detection unit comprises a sample injection groove, a first reagent injection groove, a merging groove, a second reagent injection groove, a mixing groove and a reaction groove which are arranged on a substrate; the sample injection groove is arranged at a position close to the circle center of the chip body, and the first reagent injection groove, the flow combination groove, the mixing groove and the reaction groove are sequentially arranged along a direction far away from the circle center of the chip body; the second reagent injection groove is located on the side of the confluence groove. The utility model discloses a centrifugal micro-fluidic chip for instant mixing and detection, which combines a micro-fluidic technology with a traditional biochemical detection method and can more effectively and conveniently realize the detection of biochemical samples.
Description
Technical Field
The utility model relates to the technical field of microfluidic chips, in particular to a centrifugal microfluidic chip for instant mixing and detection.
Background
Biochemical detection is generally a method of qualitatively or quantitatively detecting and analyzing a response generated by a target analyte in a selective manner by a specific biochemical reaction using optical, electrochemical, chromatographic, or the like means. Among them, the photochemical development method is one of the most convenient and effective biochemical detection methods due to its advantages of low cost, simple method, fast response, etc.
The microfluidic technology is a technology for controlling fluid at a micron scale, and can integrate a plurality of operation units related to biochemical analysis, such as sample treatment, separation, detection and the like, through a microchannel network, so that the consumption of sample reagents can be reduced, and the detection efficiency can be improved.
The photochemical sensor is a relatively simple and rapid method in biochemical detection, and has a relatively wide application prospect. However, it usually involves multiple operations such as sample loading, mixing, and detection, and the procedure of tube coagulation detection is usually: taking a proper amount of plasma sample, adding the reagent 1, uniformly mixing the sample to be detected and the reagent 1, adding the reagent 2, uniformly mixing and detecting. The traditional biochemical detection steps are complex, the reagent consumption is high, and high-throughput detection is difficult to realize, which limits the application of the biochemical detection in the field of instant detection to a certain extent, so that further improvement and development are needed to realize more convenient and faster analysis and detection with lower cost.
Therefore, how to provide a centrifugal microfluidic chip for instant mixing and detection is a problem that needs to be solved by those skilled in the art.
SUMMERY OF THE UTILITY MODEL
In view of the above, the present invention provides a centrifugal microfluidic chip for instant mixing and detection, so as to perform various high-throughput analysis and detection efficiently and rapidly.
In order to realize the scheme, the utility model adopts the following technical scheme:
a centrifugal micro-fluidic chip for instant mixing and detection comprises a substrate and a cover plate, wherein the cover plate and the substrate are in sealing fit to form a chip body; the chip body is provided with a plurality of mixed detection units which take the circle center of the chip body as an original point and are distributed in a radial shape;
the mixing detection unit comprises a sample injection groove, a first reagent injection groove, a confluence groove, a second reagent injection groove, a mixing groove and a reaction groove which are arranged on the substrate;
the sample injection groove is arranged at a position close to the circle center of the chip body, and the first reagent injection groove, the flow combination groove, the mixing groove and the reaction groove are sequentially arranged along a direction far away from the circle center of the chip body; the second reagent injection groove is positioned on one side of the confluence groove;
the sample injection groove is communicated with the confluence groove through a first channel, and one end of the first reagent injection groove, which is far away from the circle center of the chip body, is communicated with the confluence groove; the confluence groove is communicated with the mixing groove through a second channel; the second reagent injection groove is communicated with the mixing groove through a third channel; the mixing tank is communicated with the reaction tank through a fourth channel.
Preferably, in the above centrifugal microfluidic chip for mixing and detecting instantly, the mixing detection unit further comprises a sample addition hole, a first reagent addition hole, and a second reagent addition hole, which are disposed on the cover plate, wherein the sample addition hole corresponds to and communicates with the sample injection groove, the first reagent addition hole corresponds to and communicates with the first reagent injection groove, and the second reagent addition hole corresponds to and communicates with the second reagent injection groove.
Preferably, in the centrifugal microfluidic chip for instant mixing and detection, the sample injection groove, the first reagent injection groove, the confluence groove, the second reagent injection groove, the mixing groove, the reaction groove, the first channel, the second channel, the third channel and the fourth channel are all groove structures.
Preferably, in the centrifugal microfluidic chip for instant mixing and detection, the center of the cover plate is provided with a cover plate fixing hole, and the center of the substrate is provided with a substrate fixing hole; the cover plate fixing holes and the substrate fixing holes are identical in shape and are arranged in a superposed mode, and chip fixing holes penetrating through the chip body are formed.
Preferably, in the centrifugal microfluidic chip for instant mixing and detection, the cover plate and the substrate are made of one of silicon wafer, quartz, glass and polymer compound.
Preferably, in the centrifugal microfluidic chip for instant mixing and detection, the polymer compound is polymethacrylate, polystyrene, cyclic olefin copolymer or polycarbonate.
According to the technical scheme, compared with the prior art, the centrifugal microfluidic chip for instant mixing and detection is provided, and the detection of biochemical samples can be more effectively and conveniently realized by combining the microfluidic technology with the traditional biochemical detection method. Simultaneously, the method also has the following beneficial effects:
1. the multi-channel unit parallel microfluidic chip is adopted, so that various samples and/or various indexes can be detected at the same time, and high-throughput detection can be realized;
2. through a plurality of expansion-contraction structures in the microchannel unit, the mixing reaction between the sample and the first reagent can be better realized, after the second reagent is added, good mixing effect can be obtained only by carrying out one-time forward and backward oscillation, the operation steps are simplified, the requirement on the performance aspect of the centrifuge is reduced, and the microchannel unit is suitable for biochemical instant detection.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the provided drawings without creative efforts.
FIG. 1 is a schematic diagram of the structure of the present invention;
fig. 2 is a schematic structural diagram of the hybrid detection unit.
Detailed Description
The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The embodiment of the utility model discloses a centrifugal microfluidic chip for instant mixing and detection, which comprises a substrate and a cover plate, wherein the cover plate is in sealing fit with the substrate to form a chip body 1; the chip body 1 is provided with a plurality of mixed detection units 2, and the plurality of mixed detection units 2 are distributed in a radial shape by taking the circle center of the chip body 1 as an origin;
the mixing detection unit 2 comprises a sample injection groove 3, a first reagent injection groove 4, a confluence groove 5, a second reagent injection groove 6, a mixing groove 7 and a reaction groove 8 which are arranged on a substrate;
the sample injection groove 3 is arranged at a position close to the circle center of the chip body 1, and the first reagent injection groove 4, the confluence groove 5, the mixing groove 7 and the reaction groove 8 are sequentially arranged along the direction far away from the circle center of the chip body 1; the second reagent injection groove 6 is positioned at one side of the confluence groove 5;
the sample injection groove 3 is communicated with the confluence groove 5 through a first channel 9, and one end of the first reagent injection groove 4, which is far away from the circle center of the chip body 1, is communicated with the confluence groove 5; the confluence groove 5 is communicated with the mixing groove 7 through a second channel 10; the second reagent injection groove 6 is communicated with the mixing groove 7 through a third channel 11; the mixing tank 7 communicates with the reaction tank 8 through a fourth passage 12.
In order to further optimize the above technical solution, the hybrid detection unit 2 further includes a sample addition hole 13, a first reagent addition hole 14, and a second reagent addition hole 15, which are disposed on the cover plate, the sample addition hole 13 corresponds to and communicates with the sample injection groove 3, the first reagent addition hole 14 corresponds to and communicates with the first reagent injection groove 4, and the second reagent addition hole 15 corresponds to and communicates with the second reagent injection groove 6.
In order to further optimize the technical scheme, the sample injection groove 3, the first reagent injection groove 4, the confluence groove 5, the second reagent injection groove 6, the mixing groove 7, the reaction groove 8, the first channel 9, the second channel 10, the third channel 11 and the fourth channel 12 are all groove structures.
In order to further optimize the technical scheme, a cover plate fixing hole is formed in the center of the cover plate, and a base plate fixing hole is formed in the center of the base plate; the cover plate fixing hole is identical to the base plate fixing hole in shape and is arranged in a superposed mode, a chip fixing hole 16 penetrating through the chip body 1 is formed, and the cover plate fixing hole is used for being matched with centrifugal rotating equipment to be installed through the chip fixing hole 16 to enable the chip to rotate.
In order to further optimize the technical scheme, the cover plate and the substrate are made of one of silicon wafers, quartz, glass and high molecular compounds.
In order to further optimize the technical scheme, the high molecular compound is polymethacrylate, polystyrene, cyclic olefin copolymer or polycarbonate.
The detection method comprises the following steps:
adding a sample solution and a first reagent into the sample injection groove 3 and the first reagent injection groove 4 through the sample addition hole 13 and the first reagent addition hole 14 respectively, controlling the chip body 1 to operate at the rotating speed of 1000RPM for 40s, enabling the sample and the first reagent to flow into the mixing groove 7 through the confluence groove 5 and finally reach the reaction groove 8, and enabling a plurality of expansion-contraction structures in the micro-channel unit to be fully mixed, wherein the expansion-contraction structures refer to expansion-contraction structures formed by the sample injection groove 3, the first reagent injection groove 4, the confluence groove 5, the second reagent injection groove 6, the mixing groove 7, the reaction groove 8, the first channel 9, the second channel 10, the third channel 11 and the fourth channel 12 due to different volumes and sections;
the second reagent is injected into the second reagent injection groove 6, the operation of the chip body 1 is controlled at 1500RPM for 20s, and the second reagent can be transferred to the reaction groove 8.
The second reagent and other solutions can be uniformly mixed in the reaction tank 8 by controlling the chip body 1 to rotate forward and backward for 5s at the rotation speed of 1000RPM and the conversion time of 0.5 s.
The reacted liquid can be visually observed or optically detected in the reaction tank 8.
The embodiments in the present description are described in a progressive manner, each embodiment focuses on differences from other embodiments, and the same and similar parts among the embodiments are referred to each other. The device disclosed by the embodiment corresponds to the method disclosed by the embodiment, so that the description is simple, and the relevant points can be referred to the method part for description.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the utility model. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (6)
1. A centrifugal micro-fluidic chip for instant mixing and detection is characterized by comprising a substrate and a cover plate, wherein the cover plate and the substrate are in sealing fit to form a chip body; the chip body is provided with a plurality of mixed detection units which take the circle center of the chip body as an original point and are distributed in a radial shape;
the mixing detection unit comprises a sample injection groove, a first reagent injection groove, a confluence groove, a second reagent injection groove, a mixing groove and a reaction groove which are arranged on the substrate;
the sample injection groove is arranged at a position close to the circle center of the chip body, and the first reagent injection groove, the flow combination groove, the mixing groove and the reaction groove are sequentially arranged along a direction far away from the circle center of the chip body; the second reagent injection groove is positioned on one side of the confluence groove;
the sample injection groove is communicated with the confluence groove through a first channel, and one end of the first reagent injection groove, which is far away from the circle center of the chip body, is communicated with the confluence groove; the confluence groove is communicated with the mixing groove through a second channel; the second reagent injection groove is communicated with the mixing groove through a third channel; the mixing tank is communicated with the reaction tank through a fourth channel.
2. The microfluidic chip of claim 1, wherein the mixing and detecting unit further comprises a sample well, a first reagent well and a second reagent well disposed on the cover plate, the sample well corresponds to and communicates with the sample injection slot, the first reagent well corresponds to and communicates with the first reagent injection slot, and the second reagent well corresponds to and communicates with the second reagent injection slot.
3. The microfluidic chip of claim 1, wherein the sample injection groove, the first reagent injection groove, the confluence groove, the second reagent injection groove, the mixing groove, the reaction groove, the first channel, the second channel, the third channel, and the fourth channel are all groove structures.
4. The centrifugal microfluidic chip for instant mixing and detection according to claim 1, wherein the cover plate is centrally provided with a cover plate fixing hole, and the base plate is centrally provided with a base plate fixing hole; the cover plate fixing holes and the substrate fixing holes are identical in shape and are arranged in a superposed mode, and chip fixing holes penetrating through the chip body are formed.
5. The microfluidic chip of claim 1, wherein the cover plate and the substrate are made of one of silicon wafer, quartz, glass, and polymer.
6. The microfluidic chip of claim 5, wherein the polymer compound is polymethacrylate, polystyrene, cyclic olefin copolymer, or polycarbonate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121234872.6U CN215506825U (en) | 2021-06-03 | 2021-06-03 | Centrifugal micro-fluidic chip for instant mixing and detection |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121234872.6U CN215506825U (en) | 2021-06-03 | 2021-06-03 | Centrifugal micro-fluidic chip for instant mixing and detection |
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| Publication Number | Publication Date |
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| CN215506825U true CN215506825U (en) | 2022-01-14 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202121234872.6U Active CN215506825U (en) | 2021-06-03 | 2021-06-03 | Centrifugal micro-fluidic chip for instant mixing and detection |
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- 2021-06-03 CN CN202121234872.6U patent/CN215506825U/en active Active
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