CN215429127U

CN215429127U - Porous flat-plate liquid transfer device

Info

Publication number: CN215429127U
Application number: CN202121375600.8U
Authority: CN
Inventors: 王珏
Original assignee: Shanxi University of Chinese Mediciine
Current assignee: Shanxi University of Chinese Mediciine
Priority date: 2021-06-21
Filing date: 2021-06-21
Publication date: 2022-01-07
Anticipated expiration: 2031-06-21

Abstract

The utility model discloses a porous plate liquid transfer device, which comprises a sealing pad and a guide plate which are connected up and down, wherein the sealing pad is provided with connecting holes matched with a porous plate, and the upper end and the lower end of each connecting hole are provided with raised elastic sealing rings; the guide plate is provided with a guide hole corresponding to the connecting hole of the sealing gasket, wherein one end of the guide hole is connected with a small guide pipe, and the small guide pipe is used for being connected with a centrifugal pipe to be switched. The utility model can realize that the culture solution in the multi-hole plate is transferred to the centrifuge tube at one time for carrying out subsequent detection or identification experiments, such as batch processing and the like, without worrying about sample pollution and confusion, and realizes the purposes of sampling at any time and convenient adjustment; meanwhile, the liquid can be selectively transferred according to needs, and various experimental requirements can be met.

Description

Porous flat-plate liquid transfer device

Technical Field

The utility model belongs to the field of biological instruments, and particularly relates to a porous plate liquid transfer device.

Background

In molecular biology experiments, multi-well plates, such as 96-well bacterial culture plates, are commonly used for culturing a large number of different monoclones, after bacterial culture is finished, thalli need to be collected centrifugally, and each monoclone is subjected to related experiments such as identification, screening or protein expression. In this process, the following two problems are generally encountered:

(1) because some laboratories lack centrifuge rotors that fit into multi-well plates, particularly 96-well bacterial growth plates, it is often necessary to remove individual colonies of bacterial growth media into centrifuge tubes by pipettors, a process that takes up at least 96 tips and may contaminate and confuse each other. Meanwhile, the workload is also large;

(2) in the experiment, for the economic principle, a 96-well bacterial culture plate is fully utilized, namely, each well can culture a monoclonal thallus, but in the later screening and identification process, the selection of different time and batches is usually carried out according to different experimental requirements. Therefore, even if the 96-well bacterial culture plate can be directly centrifuged to collect the bacteria, if the bacteria sediment settled in the 96-well bacterial culture plate is not used at the same time, the situation of confusion and pollution still exists for experimenters, and the 96-well bacterial culture plate cannot be reused well because of bearing the bacteria, thereby causing unnecessary waste.

Therefore, what is needed is a pipette that can transfer all the monoclonal bacteria to separate centrifuge tubes easily, accurately and without contamination in any situation, including whether or not there is an adaptive centrifuge, and can also fully meet the requirements of subsequent experiments, such as different treatments of different samples in batches and at different times.

SUMMERY OF THE UTILITY MODEL

Utility model purpose: in order to solve the technical problems in the prior art, the utility model provides a multi-hole plate liquid transfer device which can selectively transfer substances to be transferred in a plurality of or all holes in a multi-hole plate to corresponding centrifuge tubes according to needs.

In order to achieve the purpose, the utility model provides a porous plate liquid transfer device, which comprises a sealing gasket and a guide plate which are connected up and down, wherein:

the sealing pad is provided with connecting holes matched with the liquid filling holes of the porous flat plate, and the upper end and the lower end of each connecting hole are provided with raised elastic sealing rings;

the guide plate is provided with a guide hole corresponding to the connecting hole of the sealing gasket, wherein one end of the guide hole is connected with a small guide pipe, and the small guide pipe is used for being connected with a centrifugal pipe to be switched.

The side of the guide plate is provided with an elastic buckle, the elastic buckle comprises an elastic connecting piece and a clamping plate, when the porous plate is used, the sealing gasket and the guide plate are sequentially arranged above the porous plate, the small guide pipe is positioned outside the porous plate, the bottom of the common porous plate is provided with a raised groove, and the elastic buckle can be buckled at the bottom of the porous plate by utilizing the clamping plate and the elastic connecting piece to be matched with the raised groove, so that the guide plate and the porous plate are tightly buckled. If no adaptive groove is provided, the guide plate and the porous flat plate can be tightly buckled by directly pressing.

Preferably, the side of the guide plate is provided with a row number mark and a column number mark. The row number and the column number can be set according to personal preferences, for example, the row number can be set by selecting English letters A-Z, and the column number can be set by using Arabic numerals, so that each hole can be marked, and the subsequent operation is convenient without confusion.

The elastic sealing ring protrudes from the sealing gasket by 2-3mm, and the thickness of the elastic sealing ring is 1-2 mm. Through setting up the elasticity sealing washer, can make the both ends of sealed pad respectively with treat porous flat board and the guide plate zonulae occludens who shifts.

The diameter of the bottom of the small diversion pipe is smaller than the diameter of the upper end of the centrifugal pipe to be switched by 1-3 mm.

Preferably, the outer side of the bottom of the small guide pipe is provided with an elastic ring, and the thickness of the elastic ring is 1-2 mm. Through setting up the elastic ring, can make the contact that water conservancy diversion tubule is inseparabler with the centrifuging tube of taking the switching.

The bottom of the small guide pipe is provided with a detachable plug.

When using, at first with sealed pad, the guide plate sets gradually in porous dull and stereotyped top, utilize elastic seal circle to make the three closely link to each other, the water conservancy diversion tubule is located the outside, utilize snap-on board and elastic connection spare with the inseparable lock of guide plate and porous plate, then as required, the water conservancy diversion tubule bottom that will not need not shift to the correspondence of centrifuging tube is plugged with the stopper, other water conservancy diversion tubules do not plug the stopper and directly link to each other with the centrifuging tube, reverse whole device, can shift the sample of needs to the centrifuging tube in with the realization, carry out operation on next step.

The device is particularly suitable for multi-well plate operations, such as 48-well plates, 96-well plates, and even plates with more wells.

Has the advantages that: compared with the prior art, the utility model has the following advantages:

(1) the culture solution in the multi-hole plate can be transferred to a centrifuge tube at one time for carrying out subsequent detection or identification experiments, such as batch processing and the like, so that the sample pollution and confusion are not needed to be worried about, and the purposes of sampling at any time and convenient adjustment are realized;

(2) can selectively transfer liquid according to needs, and can meet various experimental requirements.

Drawings

FIG. 1 is a top view of a 96-well plate;

FIG. 2 is a side view of a 96-well plate;

FIG. 3 is a top view of the seal plate;

FIG. 4 is a top view of a baffle;

FIG. 5 is a schematic diagram showing the positional relationship between the overall structure of the present invention and a 96-well plate.

Detailed Description

The present invention will be described in further detail with reference to specific examples, but the scope of the present invention is not limited to the examples described below.

This embodiment provides a pipettor for a 96-well plate, which is shown in fig. 1 and 2 in front view and side view, respectively, and plates of other well numbers can be similarly arranged in this manner.

Fig. 5 is a schematic view of the overall structure of the plate pipette, which includes a sealing gasket 3 and a flow guide plate 4 connected to each other from top to bottom, wherein the sealing gasket 3 is provided with connecting holes adapted to the liquid filling holes 2 of the porous plate 1, and the top and bottom ends of each connecting hole are provided with a raised elastic sealing ring 5; the guide plate 4 is provided with a guide hole 10 corresponding to the connecting hole of the sealing gasket 3, wherein one end of the guide hole is connected with a small guide pipe 6, and the small guide pipe 6 is used for connecting with a centrifugal tube 7 to be switched. The side of guide plate 4 is provided with the elasticity buckle, and the elasticity buckle includes interconnect's elastic connection spare 8 and block board 9, when using, sets gradually sealed pad, guide plate in porous dull and stereotyped top, makes the water conservancy diversion tubule 6 be located the outside simultaneously, and general porous dull and stereotyped bottom is provided with bellied groove, can utilize block board and elastic connection spare cooperation bellied groove to detain the elasticity buckle in porous dull and stereotyped bottom to with the inseparable lock of guide plate and porous dull and stereotyped. If no adaptive groove is provided, the guide plate and the porous flat plate can be tightly buckled by directly pressing.

As shown in fig. 4, the side of the baffle 4 is provided with a row number mark and a column number mark. For example, column numbers are labeled with the sequence of arabic numerals and row numbers are labeled with the english letters a-H, so that each well in a 96-well plate can be labeled.

The elastic sealing ring 5 protrudes from the sealing gasket by about 2-3mm, and the thickness of the elastic sealing ring 5 is 1-2 mm. Through setting up the elasticity sealing washer, can make the both ends of sealed pad respectively with treat porous flat board and the guide plate zonulae occludens who shifts.

The diameter of the bottom of the small guide pipe 6 is smaller than the diameter of 1-3mm of the upper end of the centrifugal tube to be switched, and in order to enable the bottom of the small guide pipe 6 to be tightly clamped with the centrifugal tube, a circle of elastic ring (not shown) can be arranged on the outer side of the bottom of the small guide pipe 6, and the thickness of the elastic ring is about 1-2mm, so that the small guide pipe 6 is in closer contact with the centrifugal tube 7 to be switched.

The bottom of each small flow duct 6 is also provided with a removable plug 11.

When the sealing gasket is used, the sealing gasket 3 and the guide plate 4 are sequentially arranged above the 96-hole flat plate, the elastic sealing ring 5 is utilized to tightly connect the sealing gasket with the 96-hole flat plate, the small guide pipe 6 is positioned at the outer side, and if the bottom of the 96-hole plate is provided with a clamping groove, the guide plate 4 and the 96-hole plate can be further tightly buckled by utilizing a clamping plate and an elastic connecting piece. Then, according to needs, the bottom of the corresponding small flow guide tube which does not need to be transferred to the centrifuge tube 7 is plugged by the plug 11, other small flow guide tubes are not plugged by the plug and are directly connected with the centrifuge tube 7, the whole device is inverted, and then the required sample can be transferred to the centrifuge tube for next operation.

While the present invention provides many concepts and methods for a multi-well plate pipette, and many methods and ways to implement the same, it is to be understood that the above description is only a preferred embodiment of the present invention, and that various modifications and enhancements may be made by those skilled in the art without departing from the principles of the present invention, and such modifications and enhancements are also considered to be within the scope of the present invention. All the components not specified in the present embodiment can be realized by the prior art.

Claims

1. The utility model provides a porous dull and stereotyped pipettor which characterized in that, including sealed pad (3) and guide plate (4) of connecting the setting from top to bottom, wherein:

the sealing gasket (3) is provided with connecting holes matched with the liquid filling holes (2) of the porous flat plate (1), and the upper end and the lower end of each connecting hole are provided with raised elastic sealing rings (5);

the guide plate (4) is provided with a guide hole (10) corresponding to the connecting hole of the sealing gasket (3), wherein one end of the guide hole is connected with a small guide pipe (6) which is used for connecting with a centrifugal tube (7) to be switched.

2. The multi-well plate pipette according to claim 1, characterized in that the side of the flow guide plate (4) is provided with an elastic snap comprising an elastic connector (8) and a snap plate (9) connected to each other.

3. The multi-well plate pipette according to claim 1, characterized in that the side edges of the flow guide plate (4) are provided with row number marks and column number marks.

4. The multi-well plate pipette according to claim 1, wherein the elastic sealing ring (5) protrudes from the gasket by 2-3mm, and the thickness of the elastic sealing ring (5) is 1-2 mm.

5. The multi-well plate pipette according to claim 1, wherein the diameter of the bottom of the flow-guiding small tube (6) is 1-3mm smaller than the diameter of the upper end of the centrifuge tube to be transferred.

6. The multi-well plate pipette according to claim 1, wherein an elastic ring is arranged outside the bottom of the flow guide small tube (6), and the thickness of the elastic ring is 1-2 mm.

7. The multi-well plate pipette according to claim 1, wherein the bottom of the flow-directing vial (6) is provided with a removable plug (11).