CN215250836U - Sampling device for preparing stem cell samples - Google Patents

Abstract

The utility model innovatively provides a sampling device for preparing stem cell samples, which comprises a box body and a box cover, a reagent tube and a sampling mechanism are arranged in the box body, the sampling mechanism comprises a sampling tube and a collecting box, the sampling pipe is communicated with the collecting box, the bottom of the reagent pipe is provided with a piston cover, the lower surface of the piston cover is provided with a groove, the inner side wall of the groove is provided with internal threads, the end part of the sampling pipe is provided with a connecting block, the connecting block is provided with external threads matched with the internal threads, the sampling pipe is provided with a sampling part and a conveying part, the sampling part is arranged between the connecting block and the conveying part, the sampling part is matched with the through hole, the outer diameter of the sampling part is smaller than that of the conveying part, a plurality of first feed inlets are formed in the sampling part, a baffle ring is arranged in the sampling part, and a plurality of second feed inlets are formed in the baffle ring; utilize the connecting block to be connected with the piston lid, insert the sampling pipe and locate in the test tube, realize sealed sampling.

Description

Sampling device for preparing stem cell samples

Technical Field

The utility model relates to a sampling device's technical field, more specifically says so and relates to a preparation stem cell sample sampling device.

Background

A stem cell is a cell with unlimited or immortal self-renewal capacity, capable of producing at least one type of highly differentiated progeny cell; the sampling of the stem cells has great effect on the culture and research of the stem cells; the stem cell sampling method used in the current market mainly comprises the steps of separating stem cells by a centrifugal machine, taking the stem cells out of the centrifugal machine and sampling; in the process of sampling stem cells, the stem cells are directly contacted with air, so that the pollution of the stem cells or the activity reduction of the stem cells are easily caused, and the sampling of the stem cells is influenced.

SUMMERY OF THE UTILITY MODEL

To the deficiency that prior art exists, the utility model aims to provide a preparation stem cell sample sampling device for overcome the above-mentioned defect among the prior art.

In order to achieve the above purpose, the utility model provides a following technical scheme:

a sampling device for preparing stem cell samples comprises a box body and a box cover, wherein a reagent tube and a sampling mechanism are arranged in the box body, the sampling mechanism is arranged on the inner bottom surface of the box body and comprises a sampling tube and a collecting box, the sampling tube is communicated with the collecting box, a through hole is formed in the bottom of the reagent tube, a piston cover is arranged at the through hole, a telescopic rod is arranged in the reagent tube, one end of the telescopic rod is fixedly connected to the inner bottom surface of the reagent tube, the other end of the telescopic rod is fixedly connected to the piston cover, a groove is formed in the lower surface of the piston cover, an internal thread is arranged on the inner side wall of the groove, a connecting block is arranged at the end part of the sampling tube, an external thread matched with the internal thread is arranged on the connecting block, a sampling part and a conveying part are arranged on the sampling tube, and the sampling part is arranged between the connecting block and the conveying part, sampling portion and through-hole phase-match, the sampling portion external diameter is less than the transport portion external diameter, be provided with the first feed inlet of a plurality of in the sampling portion, be provided with in the sampling portion and keep off the ring, keep off and be provided with a plurality of second feed inlet on the ring, be provided with first power supply in the sampling portion, first power supply drive keeps off the ring and rotates, so that first feed inlet is opened or is closed, be provided with second power supply and third power supply on the sampling pipe, the second power supply sets up on the third power supply, sampling pipe fixed connection is on the second power supply, second power supply drive sampling pipe rotates, so that the sampling pipe, third power supply drive sampling pipe moves in vertical direction, so that sampling pipe business turn over reagent pipe.

Preferably, the piston cover comprises a limiting end and a sealing end, the sealing end is matched with the through hole, the limiting end is arranged above the sealing end, and the outer diameter of the limiting end is larger than that of the sealing end.

Preferably, the reagent tube is provided with a limiting groove communicated with the through hole, and the outer side wall of the sealing end is provided with a limiting block communicated with the limiting groove.

Preferably, a plurality of telescopic grooves are formed in the reagent tube, a plurality of telescopic rods are correspondingly arranged in the telescopic grooves, one end of each telescopic rod is fixedly connected to the lower bottom surface of each telescopic groove, and the other end of each telescopic rod is fixedly connected to the limiting end.

Preferably, a reaction mechanism is further arranged in the box body and comprises a reaction box, a first guide pipe and a second guide pipe, the reaction box is arranged at the bottom of the box cover, the reaction box is communicated with the reagent pipe through a connecting pipe, the first guide pipe and the second guide pipe are both arranged on the upper surface of the box cover, the first guide pipe and the second guide pipe penetrate through the box cover and extend into the reaction box, and the second guide pipe arranged outside the reaction box is connected with a reagent.

Preferably, a stirring piece is arranged in the reaction box and comprises a stirring rod and stirring blades, the stirring rod is vertically arranged in the reaction box, and one end of the stirring rod penetrates through the box cover and extends to the upper surface of the box cover.

Preferably, the bottom of the reaction box is provided with a fourth power source, an output end of the fourth power source is provided with an input gear, the connecting pipe is sleeved with an output gear, the input gear is meshed with the output gear, the connecting pipe is rotatably connected to the reaction box, and the fourth power source drives the input gear to rotate so as to enable the reagent tube to rotate.

The utility model has the advantages that: the sampling tube samples stem cells in the reagent tube, and the piston cover is used for sealing the reagent tube, so that liquid in the reagent tube is prevented from leaking in the using process, sealed sampling is realized, and stem cells are prevented from contacting air to cause stem cell pollution; the piston cover is connected with the sampling pipe through threads, so that the piston cover is detachably connected with the sampling pipe, and meanwhile, the connection stability between the piston cover and the sampling pipe is ensured; be provided with the spacing groove on the reagent pipe, be provided with the stopper on the piston cover, utilize the cooperation of stopper and spacing groove, avoid taking place relative rotation between piston cover and the reagent pipe.

Drawings

FIG. 1 is an overall structure of the present invention;

FIG. 2 is a structural diagram of a sampling tube of the present invention;

FIG. 3 is a diagram of the piston cap of the present invention;

FIG. 4 is a diagram illustrating the usage status of the present invention;

fig. 5 is a schematic structural diagram of the reaction mechanism of the present invention.

Reference numerals: 1. a box body; 2. a box cover; 3. a reagent tube; 31. a piston cap; 311. a groove; 312. a limiting end; 313. sealing the end; 314. a limiting block; 32. a telescopic rod; 321. a telescopic groove; 33. a limiting groove; 4. A sampling mechanism; 41. a sampling tube; 411. connecting blocks; 412. a sampling section; 413. a first feed port; 414. A conveying section; 415. a baffle ring; 416. a second feed port; 42. a collection box; 5. a reaction mechanism; 51. a reaction box; 52. a connecting pipe; 53. a first conduit; 54. a second conduit; 55. a reagent box; 56. and (4) a stirring piece.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

It will be understood that when an element is referred to as being "secured to" another element, it can be directly on the other element or intervening elements may also be present. When a component is referred to as being "connected" to another component, it can be directly connected to the other component or intervening components may also be present. When a component is referred to as being "disposed on" another component, it can be directly on the other component or intervening components may also be present. The terms "vertical," "horizontal," "left," "right," and the like as used herein are for illustrative purposes only.

Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs. The terminology used in the description of the invention herein is for the purpose of describing particular embodiments only and is not intended to be limiting of the invention. As used herein, the term "and/or" includes any and all combinations of one or more of the associated listed items.

The embodiments of the present invention will be described in detail with reference to the accompanying drawings:

as shown in fig. 1-5, a sampling device for preparing stem cells comprises a box body 1 and a box cover 2, a reagent tube 3, a sampling mechanism 4 and a reaction mechanism 5 are arranged in the box body 1, the reaction mechanism 5 is used for carrying out reaction and separation on blood, the reacted blood enters the reagent tube 3 through a connecting tube 52, and the stem cells are separated under the centrifugal action; extracting the stem cells in the reagent tube 3 by using a sampling mechanism 4; the separation and sampling of the stem cells are carried out in a sealed state, so that the condition that the blood is polluted and the activity of the stem cells is influenced in the operation process is avoided.

As shown in fig. 1, the reagent tube 3 is vertically arranged in the box body 1, the sampling mechanism 4 is arranged on the inner bottom surface of the box body 1, the sampling mechanism 4 comprises a sampling tube 41 and a collecting box 42, the collecting box 42 is arranged on one side of the box body 1, and the sampling tube 41 penetrates through the box body 1 and extends into the box body 1; a through hole is formed in the bottom of the reagent tube 3, a piston cover 31 is arranged in the through hole, the piston cover 31 comprises a limiting end 312 and a sealing end 313, the sealing end 313 is matched with the through hole, and the sealing end 313 is inserted into the through hole; the limiting end 312 is arranged above the sealing end 313, and the limiting end 312 is arranged in the reagent tube 3; the outer diameter of the limiting end 312 is larger than that of the sealing end 313, the sealing end 313 is inserted into the through hole, liquid in the reagent tube 3 is prevented from leaking in the using process, and the limiting end 312 is used for preventing the piston cover 31 from being separated from the reagent tube 3 in the moving process; a plurality of telescopic grooves 321 are arranged in the reagent tube 3, one end of the telescopic rod 32 correspondingly arranged in the telescopic grooves 321 is fixedly connected to the lower bottom surface of the telescopic grooves 321, and the other end of the telescopic rod 32 is fixedly connected to the limiting end 312; when the telescopic rod 32 is arranged in the telescopic groove 321, the piston cover 31 is arranged in the through hole in a sealing manner; when the piston cap 31 reaches the outside of the telescopic slot 321 extended by the telescopic rod 32, the piston cap 31 is separated from the through hole and is arranged in the reagent tube 3, and the telescopic rod 32 is utilized to prevent the piston cap 31 from rotating in the moving process of the piston cap 31, and simultaneously, the piston cap 31 is ensured to be arranged in the through hole which can be reset.

As shown in fig. 1-4, a groove 311 is formed in the lower surface of the piston cap 31, an internal thread is formed on the inner side wall of the groove 311, a connection block 411 is arranged at the upper end of the sampling tube 41, an external thread is formed on the outer surface of the connection block 411, and the internal thread is matched with the external thread, so that the piston cap 31 and the sampling tube 41 can be fixedly connected, and the piston cap 31 can be conveniently mounted and dismounted; the sampling tube 41 comprises a sampling part 412 and a conveying part 414, the sampling part 412 is arranged between the conveying part 414 and the connecting block 411, the outer diameter of the sampling part 412 is matched with the inner diameter of the through hole, and the outer diameter of the conveying part 414 is larger than the outer diameter of the sampling part 412; ensuring that the sampling part 412 can be inserted into the through hole in the sampling process, and meanwhile, the conveying part 414 is pressed against the lower surface of the reagent tube 3 and is connected in a sealing manner, so that liquid in the reagent tube 3 is prevented from leaking; a plurality of first feed ports 413 are arranged on the sampling part 412, a baffle ring 415 is arranged in the sampling part 412, a second feed port 416 is arranged on the baffle ring 415, a first power source is arranged in the sampling part 412, the baffle ring 415 is driven to rotate in the sampling part 412 by the first power source, when the first feed port 413 is communicated with the second feed port 416, liquid outside the sampling pipe 41 cannot enter the sampling part 412, and when the first feed port 413 is staggered with the second feed port 416, the baffle ring 415 covers the first feed port 413 to realize sealing; a second power source and a third power source are arranged on the sampling pipe 41, the second power source is arranged on the third power source, and the third power source is arranged on the lower bottom surface of the lower box body 1; the sampling pipe 41 is driven to rotate by a second power source, so that the piston cover 31 is connected and detached with the connecting block 411 in a threaded manner; driving the sampling pipe 41 to move in the vertical direction by using a third power source, so that the sampling pipe 41 enters and exits the reagent pipe 3; the sampling pipe 41 is also provided with a telescopic pipe which is arranged at the lower end of the conveying part 414, the collecting box 42 is connected with the sampling pipe 41 through the telescopic pipe, the telescopic pipe is hermetically connected with the box body 1, and the telescopic pipe is used for ensuring that the sampling pipe 41 is always communicated with the telescopic pipe in the movement process.

A limiting groove 33 is formed in the inner wall of the reagent tube 3, the limiting groove 33 is communicated with the through hole, a baffle is arranged at the lower end of the limiting groove 33, a limiting block 314 matched with the limiting groove 33 is arranged on the outer side wall of the sealing end 313, and the piston cover 31 cannot rotate relative to the reagent tube 3 in the process that the fixing block rotates relative to the piston cover 31 by means of the matching of the limiting block 314 and the limiting groove 33; the lower end of the limit groove 33 is provided with a baffle plate to ensure that the liquid in the reagent tube 3 does not leak through the limit groove 33 when the sampling part 412 is inserted into the through hole.

As shown in fig. 1 and 5, the reaction mechanism 5 includes a reaction box 51, a first conduit 53 and a second conduit 54, the reaction box 51 is disposed at the bottom of the box cover 2, the reaction box 51 is communicated with the reagent tube 3 through a connecting tube 52, the first conduit 53 and the second conduit 54 are disposed on the upper surface of the box cover 2, the first conduit 53 and the second conduit 54 both penetrate through the box cover 2 and extend into the reaction box 51, and the second conduit 54 disposed outside the reaction box 51 may be connected with a reagent box 55; the blood can be conveyed into the reaction box 51 through the first conduit 53, the separation liquid is stored in the reaction box 51 and conveyed into the reaction box 51 through the second conduit 54, and the blood can be always in a vacuum state in the sampling and separating process; a stirring piece 56 is arranged in the reaction box 51, the stirring piece 56 comprises a stirring rod and a stirring blade, the stirring rod is vertically arranged in the reaction box 51, and one end of the stirring rod penetrates through the box cover 2 and extends to the upper surface of the box cover 2; the stirring member 56 is used to make the reaction between the blood and the separation liquid more sufficient; a fourth power source is arranged at the bottom of the reaction box 51, an input gear is arranged at the output end of the fourth power source, an output gear is sleeved on the connecting pipe 52, the input gear is meshed with the output gear, the connecting pipe 52 is rotationally connected to the reaction box 51, and the fourth power source drives the input gear to rotate so as to enable the reagent tube 3 to rotate; driving the reagent tube 3 to rotate by using a fourth power source, and layering blood in the reagent tube 3 under the action of centrifugal force; the box body 1 is also internally provided with a refrigerating mechanism, and the refrigerating mechanism is utilized to regulate the temperature in the box body 1 and ensure the activity of stem cells in the separation and extraction process.

The working principle is as follows: in the use process, blood is conveyed into the reaction box 51 through the first conduit 53, the separation liquid is conveyed into the reaction box 51 through the second conduit 54, and the stirring of the blood is realized by rotating the stirring piece 56, so that the separation liquid is ensured to be more fully contacted with the blood; the reacted blood enters the reagent tube 3 through the connecting tube 52, and the reagent tube 3 is driven to rotate by the fourth power source, so that the blood in the reagent tube 3 is layered; after blood layering is completed, the sampling tube 41 is driven by the second power source and the third power source to move upwards and rotate simultaneously, so that the connecting block 411 and the piston cover 31 are fixedly connected, after connection is completed, in the process that the sampling tube 41 is driven by the third power source to be inserted into the reagent tube 3, the piston cover 31 is gradually separated from the through hole, and the telescopic rod 32 is stretched; when the reagent tube 3 is completely inserted, the first feed port 413 is communicated with the inside of the reagent tube 3, and the conveying part 414 is pressed against the lower surface of the reagent tube 3; in the process that the sampling pipe 41 is inserted into the reagent pipe 3, the sampling pipe 41 is always in sealing connection with the reagent pipe 3, so that liquid in the reagent pipe 3 is prevented from leaking; the baffle ring 415 is driven to rotate by a first power source, so that the first feed port 413 is communicated with the second feed port 416, and stem cells in the reagent tube 3 can enter the sampling tube 41 through the first feed port 413 and the second feed port 416 and enter the collection box 42; after sampling, the sampling tube 41 moves downwards under the action of the third power source, so that the telescopic rod 32 is contracted, and the sampling tube 41 is separated from the piston cap 31 under the action of the second power source and the third power source, thereby realizing separation and sampling of stem cells in a sterile environment.

Above only the utility model discloses an it is preferred embodiment, the utility model discloses a scope of protection not only limits in above-mentioned implementation, and the fan belongs to the utility model discloses a technical scheme under the thinking all belongs to the utility model discloses a scope of protection. It should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.

Claims (7)

1. The sampling device for preparing the stem cell sample comprises a box body (1) and a box cover (2), and is characterized in that a reagent tube (3) and a sampling mechanism (4) are arranged in the box body (1), the sampling mechanism (4) is arranged on the inner bottom surface of the box body (1), the sampling mechanism (4) comprises a sampling tube (41) and a collecting box (42), the sampling tube (41) is communicated with the collecting box (42), a through hole is formed in the bottom of the reagent tube (3), a piston cover (31) is arranged at the through hole, a telescopic rod (32) is arranged in the reagent tube (3), one end of the telescopic rod (32) is fixedly connected to the inner bottom surface of the reagent tube (3), the other end of the telescopic rod (32) is fixedly connected to the piston cover (31), a groove (311) is formed in the lower surface of the piston cover (31), and an internal thread is formed in the inner side wall of the groove (311), the sampling pipe (41) is provided with a connecting block (411) at the end part, the connecting block (411) is provided with an external thread matched with the internal thread, the sampling pipe (41) is provided with a sampling part (412) and a conveying part (414), the sampling part (412) is arranged between the connecting block (411) and the conveying part (414), the sampling part (412) is matched with the through hole, the outer diameter of the sampling part (412) is smaller than the outer diameter of the conveying part (414), the sampling part (412) is provided with a plurality of first feed inlets (413), the sampling part (412) is provided with a baffle ring (415), the baffle ring (415) is provided with a plurality of second feed inlets (416), the sampling part (412) is provided with a first power source, the first power source drives the baffle ring (415) to rotate so as to open or close the first feed inlet (413), the sampling pipe (41) is provided with a second power source and a third power source, the second power supply sets up on the third power supply, sampling pipe (41) fixed connection is on the second power supply, second power supply drive sampling pipe (41) rotate, so that sampling pipe (41), third power supply drive sampling pipe (41) move in vertical direction, so that sampling pipe (41) business turn over reagent pipe (3).

2. The device for preparing a sample of stem cells according to claim 1, wherein the piston cap (31) comprises a stopper end (312) and a sealing end (313), the sealing end (313) is matched with the through hole, the stopper end (312) is disposed above the sealing end (313), and the outer diameter of the stopper end (312) is larger than that of the sealing end (313).

3. The device for sampling the stem cell samples according to claim 2, wherein the reagent tube (3) is provided with a limiting groove (33) communicated with the through hole, and the outer side wall of the sealing end (313) is provided with a limiting block (314) communicated with the limiting groove (33).

4. The sampling device for preparing stem cell samples according to claim 2, wherein a plurality of telescopic slots (321) are arranged in the reagent tube (3), a plurality of telescopic rods (32) are correspondingly arranged in the telescopic slots (321), one end of each telescopic rod (32) is fixedly connected to the lower bottom surface of each telescopic slot (321), and the other end of each telescopic rod (32) is fixedly connected to the limiting end (312).

5. The stem cell sample preparation sampling device according to claim 1, wherein a reaction mechanism (5) is further disposed in the box body (1), the reaction mechanism (5) comprises a reaction box (51), a first conduit (53) and a second conduit (54), the reaction box (51) is disposed at the bottom of the box cover (2), the reaction box (51) and the reagent tube (3) are communicated through a connecting tube (52), the first conduit (53) and the second conduit (54) are both disposed on the upper surface of the box cover (2), the first conduit (53) and the second conduit (54) both penetrate through the box cover (2) and extend into the reaction box (51), and the second conduit (54) disposed outside the reaction box (51) is connected with a reagent box (55).

6. The sampling device for preparing stem cell samples according to claim 5, wherein a stirring member (56) is arranged in the reaction box (51), the stirring member (56) comprises a stirring rod and a stirring blade, the stirring rod is vertically arranged in the reaction box (51), and one end of the stirring rod penetrates through the box cover (2) and extends to the upper surface of the box cover (2).

7. The device for sampling stem cell samples according to claim 5, wherein a fourth power source is arranged at the bottom of the reaction box (51), an input gear is arranged at the output end of the fourth power source, an output gear is sleeved on the connecting pipe (52), the input gear is meshed with the output gear, the connecting pipe (52) is rotatably connected to the reaction box (51), and the fourth power source drives the input gear to rotate, so that the reagent pipe (3) rotates.

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