CN215234332U

CN215234332U - Novel 3D cultivates and uses orifice plate frame

Info

Publication number: CN215234332U
Application number: CN202022853633.0U
Authority: CN
Inventors: 郑晓佳
Original assignee: Tianjin Kangting Biological Engineering Group Co Ltd
Current assignee: Tianjin Kangting Biological Engineering Group Co Ltd
Priority date: 2020-12-02
Filing date: 2020-12-02
Publication date: 2021-12-21
Anticipated expiration: 2030-12-02

Abstract

The utility model relates to a novel 3D cultivates and forms, every by the mutual cartridge concatenation of a plurality of unit hole grillage is including supporting base and orifice plate, it is equipped with the cartridge base on the base to support admittedly, the lead screw is installed to cartridge base upper surface bilateral symmetry, the equal screw thread in orifice plate both ends is installed on the lead screw, the coaxial gear that is equipped with in lead screw one end lower part, connect through the tooth chain transmission between the gear, still coaxial drive gear that is equipped with admittedly on the lead screw on gear upper portion, still be provided with drive gear on cartridge base upper surface one side, the coaxial rocking handle that is equipped with admittedly on the drive gear, drive gear and drive gear meshing are connected. The utility model discloses a thereby the orifice plate height of regulation unit orifice plate frame forms whole orifice plate frame and is the echelonment structure, does not shelter from all around, is convenient for see the actual conditions in each orifice plate clearly, is convenient for experiment operation and observation.

Description

Novel 3D cultivates and uses orifice plate frame

Technical Field

The utility model belongs to the technical field of experiment auxiliary assembly, a hole grillage is related to, especially a novel 3D cultivates and uses hole grillage.

Background

At present, 96-well plates used in laboratories and research institutions comprise enzyme label plates, microplate plates, cell culture plates and the like, experimental operations and experimental results in the plates need to be observed in many experimental processes, even operation needs to be carried out at a certain fixed position in the plates, and the operations are fine and step by step and are difficult to achieve by the conventional common 96-well plates;

in the existing 3D culture of cells, the cells are very hot, but in the culture process, hydrogel is used for 3D culture of the cells, in the operation process, the boundary points of the gel and an upper layer culture medium need to be distinguished, the gel surface cannot be touched when the culture medium is replaced, otherwise the growth environment of the cells can be damaged, and cell balls are broken, but the boundary points of the gel and the upper clear in each hole are difficult to observe in the existing 96-well plate, so that the success rate of experimental operation is influenced, and the accuracy of experimental results is finally influenced.

The following patent documents relevant to the present application are found by search, and specific disclosures are as follows:

1. a96-hole plate frame (CN207405185U) comprises a fixed frame, two slideways and two marking points, wherein the fixed frame is provided with a groove structure for placing a 96-hole plate; the two slideways are positioned on two sides of the fixed frame groove and used as sliding tracks of the mark points; the two marking points are respectively embedded in the two slideways, can move in the horizontal or vertical direction, can correspond to the positions of 96 holes on a 96-hole plate, and are used for indicating the position of a next hole to be sampled; after sample adding, an experimenter moves the mark points in the two horizontal and vertical slide ways on the fixing frame to the position of the corresponding next hole to be added with the sample, and then extracts another sample to add the sample; the fixing frame, the slide way and the mark points are all made of glass materials and can be used repeatedly after sterilization treatment.

2. A96-well plate holder (CN210048780U) for cell culture, comprising a well plate fixing mechanism and a supporting mechanism for supporting the well plate fixing mechanism; the orifice plate fixing mechanism comprises a rectangular support plate and 4 connected baffles arranged at the edge of the support plate, wherein 3 baffles are connected with a limiting block positioned on the outer side of the baffle through a connecting column, the limiting block is connected with a first spring, and the first spring is connected with an elastic sheet positioned on the inner side of the baffle; the supporting mechanism comprises a base, two parallel fixed plates arranged on the base, and a fixed plate and a fixed column which are arranged at the tops of the two fixed plates respectively, wherein two parallel baffle plates with elastic pieces are fixedly connected with a first rotating shaft and a second rotating shaft respectively, the first rotating shaft penetrates through the fixed plate and can rotate relative to the fixed plate, a nut used for fixing the first rotating shaft is sleeved on the first rotating shaft, and the second rotating shaft penetrates through the fixed column and can rotate relative to the fixed column.

Through the contrast of technical characteristics, above-mentioned patent literature of disclosing with the utility model discloses a technical structure is inequality, can not influence the creativity and the novelty of the utility model application.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome prior art's weak point, provide a simple structure, convenient to use, be convenient for observe accomodate simple and convenient novel 3D cultivate with hole grillage.

The utility model provides a its technical problem take following technical scheme to realize:

a novel 3D culture pore plate frame is formed by mutually inserting and splicing a plurality of unit pore plate frames, each unit pore plate frame comprises a supporting base, an inserting base, a screw rod, a pore plate and a driving assembly, the supporting base is fixedly provided with an inserting base, the two sides of the upper surface of the inserting base are symmetrically provided with screw rods, both ends of the pore plate are installed on a screw rod in a threaded manner, a gear is coaxially and fixedly installed at the lower part of one end of the screw rod, the gears are in transmission connection through a toothed chain, a transmission gear is coaxially and fixedly arranged on a screw rod at the upper part of the gears, a driving component is also arranged on one side of the upper surface of the inserting base and comprises a driving gear and a rocking handle, the driving gear is coaxially and fixedly provided with a rocking handle, the driving gear is meshed with the transmission gear, the rocking handle drives the transmission gear to rotate, the lead screw is driven to rotate, and the height of the pore plate is adjusted.

Moreover, the diameter of the transmission gear is larger than that of the gear.

And one side of the insertion base is provided with a slot, the other side of the insertion base is provided with an insertion block matched with the slot, and the adjacent unit pore plate frames are inserted into the slot through the insertion block to realize splicing.

Further, the well plate is a plate formed with 12 culture wells, and the wells of the well plate are arranged in a row.

And a transmission protection box body is also arranged on the plug-in mounting base and wraps the transmission gear, the toothed chain and the driving gear of the driving mechanism.

And, still be provided with the orifice plate lid on the orifice plate, the orifice plate covers the upper surface and is provided with the handle, the orifice plate lid lower surface corresponds the cultivation hole of orifice plate and is provided with a plurality of rings that hang.

The utility model has the advantages that:

the utility model separately arranges each row of the pore plates, without the phenomenon of multiple rows side by side, and forms a ladder-shaped structure by adjusting the height of the pore plates of the unit pore plate frame without shielding all around, so that the actual condition in each pore plate can be clearly seen, and the experimental operation and observation are convenient;

the utility model discloses because unit hole grillage can be dismantled and assembled, cultivate the quantity in hole and select a plurality of unit holes half good according to the experiment needs, not the time spent can dismantle, reduce the volume, convenient storage.

Drawings

FIG. 1 is a schematic structural view of the present invention;

fig. 2 is a schematic cross-sectional structural view of the unit-hole plate frame of the present invention;

fig. 3 is the perspective structure schematic diagram of the unit hole plate frame of the present invention.

Detailed Description

The present invention will be described in further detail with reference to the following embodiments, which are illustrative, not restrictive, and the scope of the invention should not be limited thereto.

A novel 3D culture perforated plate frame is formed by mutually inserting and splicing a plurality of unit perforated plate frames, each unit perforated plate frame comprises a supporting base 7, an inserting base 5, a lead screw 2, a perforated plate 3 and a driving assembly, the inserting base is fixedly arranged on the supporting base, the lead screws are symmetrically arranged on two sides of the upper surface of the inserting base, the two ends of each perforated plate are in threaded installation on the lead screw, the perforated plates are horizontally arranged, a gear 10 is coaxially and fixedly arranged on the lower part of one end of the lead screw, the gears are in transmission connection through a toothed chain 9, a transmission gear 8 is coaxially and fixedly arranged on the lead screw on the upper part of the gear, the diameter of the transmission gear is larger than that of the gear, the driving assembly is further arranged on one side of the upper surface of the inserting base and comprises a driving gear 6 and a rocking handle 1, a rocking handle is coaxially and fixedly arranged on the driving gear, the driving gear is in meshing connection with the transmission gear, the driving assembly drives the transmission gear to rotate and drives the lead screw to rotate, so that the distance between the pore plate and the plug-in mounting base can be adjusted.

One side of the insertion base is provided with a slot 5-2, the other side of the insertion base is provided with an insertion block 5-1 matched with the slot, and adjacent unit pore plate frames are inserted into the slots through the insertion blocks to realize splicing;

in the practical implementation of the utility model, the pore plate is a pore plate provided with 12 culture pores, and the pores of the pore plate are arranged in a row;

the utility model discloses in the implementation, still be provided with transmission protection box 4 on the cartridge base, transmission protection box parcel drive gear, toothed chain and drive assembly's drive gear. Set up transmission protection box, can prevent that particles such as dust from entering into in, avoid gear and toothed chain card at the transmission in-process to die or the landing, play the effect of a protection to the device is whole simultaneously.

The utility model discloses in the implementation, in order to prevent that the orifice plate from receiving the dust to influence, still set up the orifice plate lid on the orifice plate, the orifice plate covers the upper surface and is provided with the handle, conveniently takes, the cultivation hole correspondence that orifice plate under the orifice plate lid surface corresponds the orifice plate is provided with a plurality of rings that hang.

The utility model discloses a pore plate can the exclusive use, also can assemble the use, the pore plate is made for transparent plastic material.

The utility model discloses in concrete operation, adopt 96 to cultivate the orifice plate in hole, at first install the orifice plate in 12 cultivation holes into unit hole grillage, form eight unit hole grillages, then form 96 orifice plates through the cartridge concatenation, when 3D cultivation experiments are done to needs, transfer the different height with the orifice plate respectively, form the 96 hole grillages of echelonment structure, then begin to experiment can.

Although the embodiments of the present invention and the accompanying drawings are disclosed for illustrative purposes, those skilled in the art will appreciate that: various substitutions, changes and modifications are possible without departing from the spirit and scope of the present invention and the appended claims, and therefore, the scope of the present invention is not limited to the disclosure of the embodiments and the accompanying drawings.

Claims

1. The utility model provides a novel 3D cultivates with orifice plate frame which characterized in that: form, every by the mutual cartridge concatenation of a plurality of unit orifice grillage including supporting base, cartridge base, lead screw, orifice plate and drive assembly, the cartridge base is equipped with on the support base admittedly, the lead screw is installed to cartridge base upper surface bilateral symmetry, the equal screw thread in orifice plate both ends is installed on the lead screw, the coaxial gear that is equipped with in lead screw one end lower part, connect through the chain-and-pinion transmission between the gear, it is equipped with drive gear still coaxial admittedly on the lead screw on gear upper portion, still be provided with drive assembly on cartridge base upper surface one side, drive assembly includes drive gear and rocking handle, the coaxial rocking handle that is equipped with admittedly on the drive gear, drive gear is connected with the drive gear meshing, and rocking handle drive gear is rotatory, and it is rotatory to drive the lead screw, adjusts the height of orifice plate.

2. The novel 3D culture well plate frame of claim 1, wherein: the diameter of the transmission gear is larger than that of the gear.

3. The novel 3D culture well plate frame of claim 1, wherein: one side of the insertion base is provided with a slot, the other side of the insertion base is provided with an insertion block matched with the slot, and adjacent unit pore plate frames are inserted into the slot through the insertion block to realize splicing.

4. The novel 3D culture well plate frame of claim 1, wherein: the pore plate is provided with 12 culture pores, and the pores of the pore plate are arranged in a row.

5. The novel 3D culture well plate frame of claim 1, wherein: and a transmission protection box body is also arranged on the insertion base and wraps the transmission gear, the toothed chain and the driving gear of the driving mechanism.

6. The novel 3D culture well plate frame of claim 1, wherein: the hole plate is also provided with a hole plate cover, the upper surface of the hole plate cover is provided with a handle, and the lower surface of the hole plate cover is correspondingly provided with a plurality of suspension rings corresponding to the culture holes of the hole plate.