CN215218276U - Centrifugal sample pretreatment device - Google Patents
Centrifugal sample pretreatment device Download PDFInfo
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- CN215218276U CN215218276U CN202121681439.7U CN202121681439U CN215218276U CN 215218276 U CN215218276 U CN 215218276U CN 202121681439 U CN202121681439 U CN 202121681439U CN 215218276 U CN215218276 U CN 215218276U
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Abstract
A centrifugal sample pretreatment device is used for biological experiments and comprises a sample liquid inlet end, a sample liquid outlet end and a weighing mechanism, wherein the sample liquid inlet end, the sample liquid outlet end and the weighing mechanism are arranged in an ice bath barrel; the sample liquid inlet end is connected with the inlet of the peristaltic pump through a pump pipe, and the outlet of the peristaltic pump is led out to form a sample liquid outlet end; the upper portion of the weighing mechanism is provided with a sampling container clamping groove, an outlet of the sample liquid outlet end is vertically arranged towards the top of the sampling container clamping groove, the sampling container clamping groove is used for placing a sampling container, and a sample is weighed through the weighing mechanism after being injected into the sampling container through the sample liquid outlet end. The utility model discloses can be fast, systematic "one-stop" accomplish centrifugal sample pretreatment overall process, including sample mixing, ration sample and quick balancing step to effectively improve centrifugal sample pretreatment operating speed, increase sample accuracy and centrifugation security. And simultaneously, as integrated device, the utility model has the characteristics of stable performance, easy and simple to handle, easy to maintain saves space.
Description
Technical Field
The utility model relates to a biology experiment technique can realize "one-stop" and accomplish a series of required preliminary treatment processes of sample before the centrifugation, mainly includes sample mixing, quantitative sampling and quick balancing step to improve centrifugal operation efficiency and security.
Background
The principle of the centrifugal operation is that the centrifugal force generated by the high-speed rotation of a centrifuge rotor is utilized to rapidly settle or float suspended particles in a sample, so that various substance components with different specific gravities are separated, and the centrifugal operation is widely used for separating suspended biological macromolecules such as cells, organelles, viruses, proteins, nucleic acids and the like in biological relevant experiments.
Based on the above technical principle, there are two important points before centrifugal operation:
1. the sample needs to be placed in the rotor symmetrically with equal weight, namely, the balancing operation is carried out, otherwise, the rotor generates larger abrasion to the rotating shaft due to different moments applied to the rotating shaft, and when the centrifugal machine runs at high speed, the service life of the centrifugal machine is shortened, and the personal safety of an operator is threatened;
2. the biological sample needs to be pretreated at room temperature before centrifugation, which mainly comprises the steps of uniformly mixing a sample suspension, quantitatively measuring a certain volume of sample and balancing, and the biological sample is exposed for a long time at room temperature to cause activity reduction and even degradation, so that the quick completion of relevant pretreatment steps is an important link for ensuring the experimental effect.
The following problems are encountered in the conventional centrifugal sample pretreatment process:
1. taking a sample: when an operator measures a certain volume of sample, the operator needs to hold the container filled with the total sample liquid to dump into the measuring cylinder, but the operation is repeated due to factors such as inaccurate sampling at one time, so that the exposure time of the sample at room temperature is prolonged, and the influence of the holding temperature easily causes the activity reduction of effective components in the sample;
2. and (3) a balancing process:
(1) when the tray is used, the left tray and the right tray swing up and down, one-time balancing can be carried out only after the swing stops, a long time is needed for waiting for the swing stop, and a knife edge is quickly abraded due to long swing time, so that the service life of the tray balance is shortened; if balancing is carried out in the swinging process, an operator cannot accurately judge whether the left end and the right end of the balance are balanced according to the equal left-right swinging amplitude, a large balancing error can be caused, and potential safety hazards are generated in the subsequent centrifuging process;
(2) when the electronic balance is used, only one sample can be weighed on the same balance, the balance between two samples can be completed only by recording the weighing readings one by one and repeatedly taking the samples for multiple times to adjust the mass of the two samples to be consistent, the process is more complicated, and an operator, particularly a beginner, easily focuses attention on the mass of the sample, so that the step of quantitatively taking the sample according to the required volume is neglected, and the experiment is difficult to continue due to the lack of the initial volume parameter of the sample;
3. no matter which step and method in the above measuring or balancing needs to be performed for a long time, especially in the process of simultaneously performing experiments by multiple persons, such as basic experiment teaching with wide audience, the phenomena of instrument waiting and stacking operation of students can be caused due to the relatively limited equipment number and long centrifugation time consumption.
The problems not only can directly influence the experimental operation efficiency and the experimental success rate, but also can even bring potential safety hazards to the centrifugal process.
Disclosure of Invention
The utility model discloses aim at realizing "one-stop" and accomplish a series of required preliminary treatment processes of sample before the centrifugation, mainly include sample mixing, quantitative sampling and quick balancing step to improve centrifugal operation efficiency and security.
In order to achieve the above purpose, the utility model discloses a technical scheme is:
a centrifugal sample pretreatment device comprises a sample liquid inlet end, a sample liquid outlet end and a weighing mechanism, wherein the sample liquid inlet end, the sample liquid outlet end and the weighing mechanism are arranged in an ice bath barrel;
the sample liquid inlet end is connected with the inlet of the peristaltic pump through a pump pipe, and the outlet of the peristaltic pump is led out to form a sample liquid outlet end;
the upper part of the weighing mechanism is provided with a sampling container clamping groove, the outlet of the sample liquid outlet end vertically faces the top of the sampling container clamping groove, the sampling container clamping groove is used for placing a sampling container, the sampling container clamping groove is made of transparent materials and is convenient for observing volume scale readings on the sampling container, and a sample is weighed by the weighing mechanism after being injected into the sampling container through the sample liquid outlet end and is displayed by a weighing display screen arranged on the weighing mechanism;
peristaltic pump electric connection drive plate and peristaltic pump control panel, through the switching and the rotational speed of peristaltic pump control panel regulation peristaltic pump to by drive plate control, and then realize that the sample pours into the sample container of placing in the sample container draw-in groove into after the sample goes out liquid end and flows out.
Further, including agitated vessel, inside sample feed liquor end and agitated vessel were vertical towards the ice bath bucket, sample feed liquor end and agitated vessel height and angle are adjustable.
Furthermore, the pump pipes are divided into two paths, two ends of each pump pipe are respectively and independently connected with the sample liquid inlet end and the inlet of the peristaltic pump, and the two paths of pump pipes are fixed together through the clamps. The sampling container clamping grooves are two and respectively correspond to the two sample outlet ends, correspondingly, the weighing mechanisms are arranged to be a left weighing mechanism and a right weighing mechanism, and the two sampling container clamping grooves are respectively positioned on the left weighing mechanism and the right weighing mechanism.
Further, the bottom of the ice bath barrel is horizontally consistent with the bottom of the weighing mechanism.
Advantageous effects
Compare traditional centrifugation sample pretreatment process flow, the utility model discloses can be quick, systematic "one-stop" accomplish centrifugation sample pretreatment overall process, including sample mixing, quantitative sample and quick balancing step to effectively improve centrifugation sample pretreatment operating speed, increase sample accuracy and centrifugation security. And simultaneously, as integrated device, the utility model has the characteristics of stable performance, easy and simple to handle, easy to maintain saves space. For production or scientific research units which often perform centrifugal experiments, the device has strong practicability and high safety, and can fully meet the requirements of experiment operation; for experimental teaching, especially for a basic experiment with wide audience, the device can effectively improve the centrifugal operation efficiency and safety, relieve the problems of long-time waiting, operation crowding and the like caused by a large number of students, reduce the risk of biological sample activity loss and sample cross contamination, improve the experimental success rate and the experimental teaching efficiency, and have higher application value.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
Fig. 2 is a perspective view of the present invention.
In the figure: 1. the device comprises a stirring device 2, a hoop 3, an ice bath barrel 4, a sample liquid inlet end 5, a pump pipe 6, a driving plate 7, a peristaltic pump 8, a peristaltic pump control panel 9, a sample liquid outlet end 10, a sampling container clamping groove 11, a weighing mechanism 12 and a weighing display screen.
Detailed Description
As can be seen from the attached drawings 1 and 2, the centrifugal sample pretreatment device of the utility model comprises a sample inlet end 4, a sample outlet end 9 and a weighing mechanism 11 which are arranged in an ice bath barrel 3; the sample liquid inlet end 4 is connected with an inlet of a peristaltic pump 7 through a pump pipe 5, and an outlet of the peristaltic pump 7 is led out to form a sample liquid outlet end 9; 11 upper portion of weighing mechanism be equipped with sampling container draw-in groove 10, the vertical top towards sampling container draw-in groove 10 of the export of sample play liquid end 9, sampling container draw-in groove 10 be used for placing the sampling container, sampling container draw-in groove 10 material is transparent, be convenient for observe the volume scale registration on the sampling container, in order to realize this purpose, this sampling container draw-in groove 10 also can be checkpost or hollow out construction, as long as can stably place the sampling container to can be convenient for observe the number of graduations on the sampling container, all accord with the utility model discloses inject. After a sample is injected into the sampling container through the sample liquid outlet end 9, the sample is weighed by the weighing mechanism 11 and is displayed by a weighing display screen 12 arranged on the weighing mechanism 11; peristaltic pump 7 electric connection drive plate 6 and peristaltic pump control panel 8, through opening and closing and the rotational speed of peristaltic pump 7 of peristaltic pump control panel 8 regulation to by drive plate 6 control, and then realize that the sample pours into the sample container of placing in the sample container draw-in groove 10 after flowing out from sample play liquid end 9.
The utility model discloses pump line 5 is two the tunnel, and two tunnel pump lines 5 are drawn together fixedly through clamp 2, and two the same peristaltic pump 7's of two the entry is connected respectively to two tunnel pump lines 5 play liquid end. The sample liquid inlet end 4 and the stirring device 1 vertically face the interior of the ice bath barrel 3, and the stirring device 1 is a cantilever type stirring rod with the rotating speed of below 1500 rpm. Agitated vessel 1 is fixed in the equipment rear through the support, has independent switch to realize the sample mixing, prevent that total sample from stewing the in-process and producing the layering inequality, improve the sample homogeneity. Sample inlet end 4 and agitated vessel 1 height and angle are adjustable, and ice bath bucket 3 is used for placing total sample container and trash ice. In a few cases, when a sample needs to be matched with water independently, the clamp 2 is removed, so that the sample liquid inlet end 4 is divided into two independent paths, namely the liquid inlet ends of the two pump pipes 5 are respectively placed into a total sample container and water.
The utility model discloses two peristaltic pumps 7 are controlled by drive plate 6 respectively to through switching and the rotational speed of peristaltic pump control panel 8 regulation peristaltic pump 7, and then realize taking a sample 9 fast samplings from the sample liquid outlet end that 7 exports of peristaltic pump were drawn forth. The sample liquid outlet end 9 is respectively vertically faced to the two sampling container clamping grooves 10, and the sampling container clamping grooves 10 are used for holding sampling containers (centrifuge tubes) placed in the sampling container clamping grooves to be in a vertical state. Sampling container draw-in groove 10 material is transparent, is convenient for observe the volume scale registration on the sampling container, realizes the ration sample. The two sampling container clamping grooves 10 are respectively positioned on the left weighing mechanism 11 and the right weighing mechanism 11, the weighing mechanisms 11 adopt electronic balance core components with one percent precision, and the weighing display screen 12 displays the total mass of sampling containers (centrifuge tubes) and samples placed on the weighing mechanisms 11 where the weighing display screens are positioned. After the samples in two same sampling containers are measured in an isometric quantitative mode, according to the mass readings displayed by the two weighing display screens 12, the opening and closing and the rotating speed of the peristaltic pump 7 on the side with smaller mass readings are adjusted through the peristaltic pump control panel 8, the mass difference between the left weighing display screen 12 and the right weighing display screen 12 is filled up by using trace samples, and accurate balancing is completed.
See fig. 2, the utility model discloses an integration equipment, equipment front side below is weighing machine 11, display screen 12 and sample container draw-in groove 10 weigh, drive plate 6, peristaltic pump 7, peristaltic pump control panel 8 is located equipment front side top, wherein peristaltic pump 7 is located inside the equipment with drive plate 6, external peristaltic pump control panel 8, the sample that draws forth from 7 exports of peristaltic pump goes out liquid end 9 and corresponds to sample container draw-in groove 10 under perpendicularly, sample feed liquor end 4, clamp 2, agitated vessel 1 and ice bath bucket 3 are located the equipment rear side, ice bath bucket 3 bottoms are unanimous with 11 bottom levels of weighing machine, pipeline 5 is located inside the device.
Through the utility model discloses pretreatment operation mode before the centrifugation of biological sample as follows:
place total sample container in ice bath bucket 3, broken ice cooling can be put into to total sample container and 3 internal clearances of ice bath bucket, in order to reduce handheld temperature and ambient temperature to the influence of biological sample activity, agitated vessel 1 that ice bath bucket 3 tops provided can go deep into total sample container, open agitated vessel 1 switch and make its operation, in order to realize the sample mixing, prevent that the sample in total sample container from producing the layering inequality at the in-process that stews, improve follow-up sample homogeneity, close the switch until the whole back that finishes of centrifugal sample pretreatment process.
Place two the same sample container (centrifuging tube) respectively in upright sample container draw-in groove 10, the setting of sample container draw-in groove 10 both can avoid handheld temperature to influence, can also make the stable vertical state that keeps of container, when avoiding the sample to spill more be convenient for observe the volume scale registration on the sample container (centrifuging tube), improves follow-up sample operation speed and accuracy.
Two sample container draw-in grooves 10 are located respectively about weighing mechanism 11, and weighing mechanism 11 can show both sides sample container respectively and the total of splendid attire sample mass separately through weighing display screen 12 about, and when sample reached the same anticipated volume (according to the volume scale registration on the sample container and judge) in the sample container (centrifuging tube) on sample container draw-in groove 10, two peristaltic pumps 7 were closed through peristaltic pump control panel 8 respectively, accomplished the ration sample.
According to the quality registration on the left and right weighing display screens 12, the switching and the rotating speed of the unilateral peristaltic pump 7 with smaller quality registration are adjusted through the peristaltic pump control panel 8, the micro samples are quickly supplemented with the mass difference of the other side, so that the quality registration of the weighing display screens 12 on the left and right weighing mechanisms 11 is the same, and accurate balancing is realized on the basis of sampling.
The utility model discloses a liquid operation is got to ration sample function also can be used for the conventionality alone, and the pipeline is convenient for wash and is changed, can not cause cross contamination between different sample uses, is favorable to further promoting the device's range of application.
Claims (5)
1. The utility model provides a centrifugation sample preprocessing device which characterized by: comprises a sample inlet end (4), a sample outlet end (9) and a weighing mechanism (11) which are arranged in an ice bath barrel (3);
the sample liquid inlet end (4) is connected with an inlet of a peristaltic pump (7) through a pump pipe (5), and an outlet of the peristaltic pump is led out to form a sample liquid outlet end (9);
the upper part of the weighing mechanism (11) is provided with a sampling container clamping groove (10), the outlet of the sample liquid outlet end (9) vertically faces the top of the sampling container clamping groove (10), the sampling container clamping groove (10) is used for placing a sampling container, the sampling container clamping groove is made of transparent materials, so that the volume scale reading on the sampling container can be observed conveniently, and a sample is weighed by the weighing mechanism (11) after being injected into the sampling container through the sample liquid outlet end (9) and is displayed by a weighing display screen (12) arranged on the weighing mechanism (11);
peristaltic pump (7) electric connection drive plate (6) and peristaltic pump control panel (8), through opening and closing and the rotational speed of peristaltic pump (7) regulation of peristaltic pump control panel (8) to by drive plate (6) control, and then realize that the sample pours into the sample container of placing in sample container draw-in groove (10) after flowing out from sample play liquid end (9).
2. The apparatus for pretreatment of centrifuged samples as in claim 1, wherein: including agitated vessel (1), inside sample feed liquor end (4) and agitated vessel (1) vertical orientation ice bath bucket (3), sample feed liquor end (4) and agitated vessel (1) height and angle are adjustable.
3. The apparatus for pretreatment of centrifuged samples as in claim 1, wherein: the pump pipes (5) are arranged into two paths, two ends of each pump pipe are respectively and independently connected with the sample liquid inlet end (4) and the inlet of the peristaltic pump (7), and the two paths of pump pipes (5) are closed and fixed through the clamps (2).
4. The apparatus for pretreatment of centrifuged samples as in claim 1, wherein: the two sampling container clamping grooves (10) are respectively corresponding to the two sample liquid outlet ends (9), correspondingly, the weighing mechanisms (11) are arranged to be a left weighing mechanism and a right weighing mechanism, and the two sampling container clamping grooves (10) are respectively positioned on the left weighing mechanism and the right weighing mechanism (11).
5. The apparatus for pretreatment of centrifuged samples as in claim 1, wherein: the bottom of the ice bath barrel (3) is horizontally consistent with the bottom of the weighing mechanism (11).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121681439.7U CN215218276U (en) | 2021-07-23 | 2021-07-23 | Centrifugal sample pretreatment device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202121681439.7U CN215218276U (en) | 2021-07-23 | 2021-07-23 | Centrifugal sample pretreatment device |
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| Publication Number | Publication Date |
|---|---|
| CN215218276U true CN215218276U (en) | 2021-12-17 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202121681439.7U Active CN215218276U (en) | 2021-07-23 | 2021-07-23 | Centrifugal sample pretreatment device |
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| CN (1) | CN215218276U (en) |
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2021
- 2021-07-23 CN CN202121681439.7U patent/CN215218276U/en active Active
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