CN215179948U

CN215179948U - Animal tissue polyamine content detection system based on HPLC

Info

Publication number: CN215179948U
Application number: CN202121590001.8U
Authority: CN
Inventors: 姜冬梅; 王泽龙; 姜坤宏; 洪斌妍; 康波
Original assignee: Sichuan Agricultural University
Current assignee: Sichuan Agricultural University
Priority date: 2021-07-13
Filing date: 2021-07-13
Publication date: 2021-12-14
Anticipated expiration: 2031-07-13

Abstract

The utility model discloses a detection system of animal tissue polyamine content based on HPLC, which belongs to the technical field of detection, and mainly comprises a first liquid storage device and a second liquid storage device, wherein the first liquid storage device and the second liquid storage device are both communicated with a mobile phase mixer, the mobile phase mixer is sequentially communicated with a mobile phase filter, a high-pressure pump, a sample injector, a chromatographic column, a detector and a recorder, and the chromatographic column is positioned in a column incubator; the mobile phase filter is sequentially provided with a first filtering cotton layer, an activated carbon layer and a second filtering cotton layer from top to bottom, one side of the first filtering cotton layer is provided with a liquid inlet, the liquid inlet is communicated with the mobile phase mixer, one side of the second filtering cotton layer is provided with a liquid outlet, and the liquid outlet is communicated with the high-pressure pump. The utility model has the advantages of simple structure, easy operation, the easy problem that pollutes and the detection accuracy is low of sample that can effectively solve existence among the prior art.

Description

Animal tissue polyamine content detection system based on HPLC

Technical Field

The utility model belongs to the technical field of detect, concretely relates to animal tissue polyamine content's detecting system based on HPLC.

Background

Polyamines (including putrescine, spermidine and spermine) are low molecular aliphatic compounds with two or more amino groups, are low molecular aliphatic nitrogen-containing compounds with biological activity generated in a biological metabolism process, are widely present in eukaryotic cells and prokaryotic cells in animal tissues, and have important effects on the digestive physiology, nutritional metabolism and normal growth of animals (particularly young animals).

High Performance Liquid Chromatography (HPLC) is a common detection means, which uses liquid as a mobile phase, adopts a high-pressure infusion system, pumps the mobile phases such as single solvents with different polarities or mixed solvents and buffer solutions with different proportions into a chromatographic column filled with a stationary phase, separates components in the column, and then enters a detector for detection, thereby realizing the analysis of a sample. The method becomes an important separation and analysis technology application in the subject fields of chemistry, medicine, industry, agriculture, commodity inspection, law inspection and the like. However, in the existing high performance liquid chromatography detection process, due to the fact that a liquid storage device provided with a mobile phase is exposed in the air for a long time, impurities or pollutants in the air easily enter a detection pipeline to cause sample pollution, and therefore the accuracy of a detection result of active substances in animal tissues is influenced. On the other hand, some products contain salt and impurities in the mobile phase, which also affect the detection result and the normal use of the instrument.

SUMMERY OF THE UTILITY MODEL

In view of the above disadvantages, the present invention provides a system for detecting polyamine content in animal tissue based on HPLC, which can effectively solve the problems of easy contamination and low detection accuracy of samples in the prior art.

In order to achieve the above object, the technical solution adopted by the present invention to solve the above problems is:

the utility model provides a detection system of animal tissue polyamine content based on HPLC, including reservoir one and reservoir two, reservoir one and reservoir two are all communicated with the mobile phase blender, and the mobile phase blender communicates with mobile phase filter, high-pressure pump, sample injector, chromatographic column, detector and record appearance in proper order, and the chromatographic column is located the column incubator;

the mobile phase filter is sequentially provided with a first filtering cotton layer, an activated carbon layer and a second filtering cotton layer from top to bottom, one side of the first filtering cotton layer is provided with a liquid inlet, the liquid inlet is communicated with the mobile phase mixer, one side of the second filtering cotton layer is provided with a liquid outlet, and the liquid outlet is communicated with the high-pressure pump;

the column incubator comprises a box body, wherein a blowing device is arranged on the upper portion of the box body, a heater, an air purification layer and a chromatographic column chamber are sequentially arranged in the box body from top to bottom, and a gas outlet is formed in the lower portion of the box body.

The utility model has the advantages that: the utility model provides a detection system of animal tissue polyamine content based on HPLC, reservoir one and reservoir two-purpose are used for the used mobile phase of deposit detection, are provided with the mobile phase filter between mobile phase blender and high-pressure pump, make the mobile phase advance in proper order and filter cotton layer one, activated carbon layer and filter cotton layer two, can fully filter and edulcorate the mobile phase, have effectively avoided the influence of impurity to the testing result, have improved the detection precision; the column incubator is provided with a heater and a blowing device which are matched for use, the temperature required by the work of the chromatographic column is maintained by heating air, and meanwhile, the column incubator is provided with an air purification layer, so that the influence of impurities contained in heated hot air on components such as the chromatographic column and detection results is avoided. The utility model has the advantages of simple structure, easy operation, the easy problem that pollutes and the detection accuracy is low of sample that can effectively solve existence among the prior art.

Furthermore, the height ratio of the first filtering cotton layer, the activated carbon layer and the second filtering cotton layer is 1-3: 2: 1-3, and preferably 1:2: 1.

The beneficial effect of adopting the further scheme is that: the height ratio of the first filtering cotton layer, the activated carbon layer and the second filtering cotton layer is proper, so that the filtering and impurity removing effects on the mobile phase can be improved.

Furthermore, a water pressure sensor and an electric pressure regulating valve are arranged on a communication pipeline between the high-pressure pump and the sample injector, and the water pressure sensor and the electric pressure regulating valve are electrically connected.

The beneficial effect of adopting the further scheme is that: the pressure of the mobile phase can be detected and adjusted by arranging the water pressure sensor and the electric pressure regulating valve, and the problem of inaccurate detection result caused by fluctuation of the flow velocity of the mobile phase when the high-pressure pump works unstably is effectively avoided.

Further, a temperature sensor is arranged on the inner side of the box body, a controller is arranged on the outer side of the box body, and the temperature sensor, the controller and the heater are electrically connected.

The beneficial effect of adopting the further scheme is that: through setting up temperature sensor and controller, be favorable to the temperature stability of column incubator.

Furthermore, the upper part of the box body is provided with a nitrogen inlet which is connected with a nitrogen bottle, the lower part of the box body is provided with a gas outlet, and the gas outlet is connected with a vacuum pump.

The beneficial effect of adopting the further scheme is that: the column temperature box is provided with nitrogen flowing, so that on one hand, nitrogen blowing can be carried out on the chromatographic column after detection is finished, and effective cooling is carried out; in addition, during detection, the whole chromatographic column is in a nitrogen protection atmosphere, and the influence of impurities in hot air on detection is further effectively avoided.

Furthermore, the periphery of the box body is provided with a heat preservation layer.

The beneficial effect of adopting the further scheme is that: through setting up the heat preservation, can prevent column incubator's heat loss, improve the accuse temperature effect.

Further, the blowing device is a fan with a motor.

The beneficial effect of adopting the further scheme is that: through the fan that sets up to have the motor for whole column temperature case is full of to hot-air, makes the temperature in the box even.

Furthermore, the box body is provided with a pore channel.

The beneficial effect of adopting the further scheme is that: through the pore channel, the pipeline connection of the chromatographic column is convenient.

The utility model provides a subassembly and connected mode that use among the detecting system if do not have special restriction and explanation, be the device that field routine used like detector and record appearance etc. and adopt conventional connected mode can.

Drawings

FIG. 1 is a schematic block diagram of a device in the inspection system of the present invention;

FIG. 2 is a schematic view of a mobile phase filter in the detection system of the present invention;

FIG. 3 is a schematic view of a cylinder oven in the detection system of the present invention;

wherein, 1, a first liquid storage device; 2. a second liquid storage device; 3. a mobile phase mixer; 4. a mobile phase filter; 41. a liquid inlet; 42. a first filtering cotton layer; 43. an activated carbon layer; 44. a liquid outlet; 45. a second filtering cotton layer; 5. a high pressure pump; 6. a water pressure sensor; 7. an electric pressure regulating valve; 8. a sample injector; 9. a column oven; 91. a box body; 92. a chromatographic column chamber; 93. an air purification layer; 94. a nitrogen inlet; 95. a gas outlet; 96. a temperature sensor; 97. a heater; 98. a controller; 99. a blowing device; 910. a duct; 10. a chromatographic column; 11. a detector; 12. a recorder.

Detailed Description

The following detailed description of the embodiments of the present invention will be made with reference to the accompanying drawings.

In an embodiment of the present invention, as shown in fig. 1-3, a system for detecting polyamine content in animal tissue based on HPLC is provided, which includes a first liquid reservoir 1 and a second liquid reservoir 2, both the first liquid reservoir 1 and the second liquid reservoir 2 are connected to a mobile phase mixer 3, the mobile phase mixer 3 is sequentially connected to a mobile phase filter 4, a high pressure pump 5, a sample injector 8, a chromatographic column 10, a detector 11 and a recorder 12, the chromatographic column 10 is located in a column oven 9; the mobile phase filter 4 is sequentially provided with a first filtering cotton layer 42, an activated carbon layer 43 and a second filtering cotton layer 45 from top to bottom, the height ratio of the first filtering cotton layer 42 to the activated carbon layer 43 to the second filtering cotton layer 45 is 1:2:1, one side of the first filtering cotton layer is provided with a liquid inlet 41, the liquid inlet 41 is communicated with the mobile phase mixer 3, one side of the second filtering cotton layer 45 is provided with a liquid outlet 44, and the liquid outlet 44 is communicated with the high-pressure pump 5; the column incubator 9 comprises a box body 91, the upper part of the box body 91 is provided with a blowing device 99, the blowing device 99 is a fan with a motor, the inside of the box body 91 is sequentially provided with a heater 97, an air purification layer 93 and a chromatographic column 10 chamber 92 from top to bottom, the lower part of the box body 91 is provided with a gas outlet 95, the inner side of the box body 91 is provided with a temperature sensor 96 (the specific model is DS18B20), the outer side of the box body 91 is provided with a controller 98 (the specific model is ohm dragon CP1H-X40DT-D PLC), the temperature sensor 96, the controller 98 and the heater 97 are electrically connected, the upper part of the box body 91 is provided with a nitrogen inlet 94, the nitrogen inlet 94 is connected with a nitrogen cylinder, the lower part of the box body 91 is provided with a gas outlet, and the gas outlet is connected with a vacuum pump, on the one hand, after the detection is finished, the nitrogen blowing can be carried out on the chromatographic column 10 for effective cooling; in addition, during detection, the whole chromatographic column 10 is in a nitrogen protection atmosphere, so that the influence of impurities in hot air on detection is further effectively avoided; the periphery of the box body 91 is provided with a heat insulation layer, and the box body 91 is provided with a pore passage 910; a water pressure sensor 6 (specifically MIK-P300) and an electric pressure regulating valve 7 (specifically ZDLM-16P-DN25) are arranged on a communication pipeline between the high-pressure pump 5 and the sample injector 8, the water pressure sensor 6 and the electric pressure regulating valve 7 are electrically connected, the pressure of the mobile phase can be detected and regulated, and the problem that the detection result is inaccurate due to fluctuation of the flow rate of the mobile phase when the high-pressure pump 5 is unstable is effectively avoided. The utility model provides a detection system of animal tissue polyamine content based on HPLC, reservoir 1 and reservoir two 2 are used for the used mobile phase of deposit detection, are provided with mobile phase filter 4 between mobile phase blender 3 and high-pressure pump 5 for the mobile phase advances in proper order and filters cotton layer 42, activated carbon layer 43 and filters cotton layer two 45, can carry out abundant filtration and edulcoration to the mobile phase, has effectively avoided the influence of impurity to the testing result, has improved the detection precision; the column oven 9 is provided with a heater 97 and an air blowing device 99 which are used in cooperation, heated air maintains the temperature required by the operation of the chromatographic column 10, and an air purification layer 93 is arranged, so that the influence of impurities contained in the heated hot air on components such as the chromatographic column 10 and detection results is avoided. The utility model has the advantages of simple structure, easy operation, the easy problem of polluting and the detection accuracy is low of the sample that can effectively solve existence among the prior art.

During operation, the first liquid storage device 1 and the second liquid storage device 2 are respectively methanol and ultrapure water, the flow rate of the high-pressure pump 5 is 1.0mL/min, the chromatographic column 10 is a SWEEL Chromstar TM C18(5 μm, 4.6 × 250mm), the detector 11 is an ultraviolet detector, and the temperature of the column incubator 9 is 30 ℃.

While the present invention has been described in detail and with reference to the accompanying drawings, it is not to be considered as limited to the scope of the invention. Various modifications and changes may be made by those skilled in the art without inventive step within the scope of the appended claims.

Claims

1. The system for detecting the polyamine content in the animal tissue based on HPLC is characterized by comprising a first liquid storage device (1) and a second liquid storage device (2), wherein the first liquid storage device (1) and the second liquid storage device (2) are both communicated with a mobile phase mixer (3), the mobile phase mixer (3) is sequentially communicated with a mobile phase filter (4), a high-pressure pump (5), a sample injector (8), a chromatographic column (10), a detector (11) and a recorder (12), and the chromatographic column (10) is positioned in a column incubator (9);

the mobile phase filter (4) is sequentially provided with a first filtering cotton layer (42), an activated carbon layer (43) and a second filtering cotton layer (45) from top to bottom, one side of the first filtering cotton layer (42) is provided with a liquid inlet (41), the liquid inlet (41) is communicated with the mobile phase mixer (3), one side of the second filtering cotton layer (45) is provided with a liquid outlet (44), and the liquid outlet (44) is communicated with the high-pressure pump (5);

column incubator (9) includes box (91), box (91) upper portion is provided with device of blowing (99), box (91) is inside from last to having set gradually heater (97), air purification layer (93) and chromatographic column room (92) down, box (91) lower part is provided with gas outlet (95).

2. The HPLC-based detection system for polyamine content in animal tissue according to claim 1, wherein the height ratio of the first filter cotton layer (42), the activated carbon layer (43) and the second filter cotton layer (45) is 1-3: 2: 1-3.

3. The HPLC-based detection system for polyamine content in animal tissue according to claim 1, wherein a water pressure sensor (6) and an electric pressure regulating valve (7) are arranged on a communication pipeline between the high-pressure pump (5) and the sample injector (8), and the water pressure sensor (6) and the electric pressure regulating valve (7) are electrically connected.

4. The system for detecting polyamine content in animal tissue based on HPLC of claim 1, wherein said box (91) is provided with a temperature sensor (96) inside, said box (91) is provided with a controller (98) outside, and said temperature sensor (96), controller (98) and said heater (97) are electrically connected.

5. The system for detecting polyamine content in animal tissues based on HPLC of claim 1, wherein said tank (91) is provided with a nitrogen inlet (94) at the upper part, said nitrogen inlet (94) is connected to a nitrogen cylinder, said tank (91) is provided with a gas outlet (95) at the lower part, and said gas outlet (95) is connected to a vacuum pump.

6. The system for detecting polyamine content in animal tissue according to claim 1, wherein said case (91) is surrounded by a heat insulating layer.

7. An HPLC-based detection system for polyamine content in animal tissue according to claim 1, wherein said blowing means (99) is a fan with motor.