CN215073623U

CN215073623U - Nucleic acid extraction and purification all-in-one machine shell assembly and nucleic acid extraction and purification all-in-one machine

Info

Publication number: CN215073623U
Application number: CN202121197138.7U
Authority: CN
Inventors: 郭敏; 赵东方; 于雪
Original assignee: Kangmaxin Shanghai Intelligent Technology Co ltd
Current assignee: Kangmaxin Shanghai Intelligent Technology Co ltd
Priority date: 2021-05-31
Filing date: 2021-05-31
Publication date: 2021-12-07
Anticipated expiration: 2031-05-31

Abstract

The utility model discloses a shell component of a nucleic acid extracting and purifying all-in-one machine, which comprises a shell, a working base, a temperature adjusting structure, a top seat and a control panel; the working base is arranged at the inner bottom of the shell, the temperature adjusting structure is arranged on at least two inner side walls of the shell, the temperature adjusting structure comprises heat insulating layers respectively clinging to the inner side walls, the top seat is arranged at the top of the shell, and the control panel is arranged in an installation groove formed in the front part of the upper end surface of the top seat; the working base, the temperature adjusting structure and the top base are enclosed to form a nucleic acid extraction working chamber; and a bin door is arranged on the front side surface of the shell. The utility model discloses can improve thermal insulation performance on the basis of original heating function, promote heating efficiency, shorten heat time, stabilize heating temperature to improve nucleic acid extraction efficiency and quality.

Description

Nucleic acid extraction and purification all-in-one machine shell assembly and nucleic acid extraction and purification all-in-one machine

Technical Field

The utility model belongs to the technical field of nucleic acid extraction and purification equipment, concretely relates to nucleic acid extraction and purification all-in-one machine casing subassembly and nucleic acid extraction and purification all-in-one.

Background

Nucleic acid is a biological macromolecular compound formed by polymerizing a plurality of nucleotides, is one of the most basic substances of life, and is widely present in all animal and plant cells and microorganisms. In recent years, with the rapid development of molecular biology, nucleic acid-based molecular diagnostic and detection techniques have increasingly highlighted important roles. Nucleic acids required for experiments and detection need to be extracted and purified from biological samples.

The existing nucleic acid extractor generally has the function of intermittently heating a sample and a reagent in a deep-hole plate so as to extract nucleic acid efficiently. However, because the space for nucleic acid extraction operation in the extraction instrument box is large, and the box is made of metal, the heat released by the heating block is easily dissipated and then leaks out through the metal box, so that the heating efficiency is influenced, the heating time is prolonged, the temperature is not kept stable, and the nucleic acid extraction quality is adversely affected. Moreover, nucleic acid extraction, such as nucleic acid extraction of new coronavirus, is very time-sensitive, i.e., the extraction speed is required to be as fast as possible, so that a detection result can be obtained at the first time, and the detection can be performed first in the course of running against an epidemic situation. In addition, the nucleic acid sample obtained by the nucleic acid extractor is generally extremely small in quantity and extremely sensitive to temperature, the conventional nucleic acid extractor does not provide a rapid cooling means, so that the nucleic acid sample is always exposed to a relatively high-temperature environment, and the nucleic acid is easily degraded or inactivated after being stored for a certain time at a temperature higher than a certain temperature, so that the accuracy of subsequent PCR detection is influenced.

Disclosure of Invention

The utility model aims at providing a nucleic acid draws purification all-in-one machine casing subassembly and nucleic acid to solve the defect mentioned in the above-mentioned background art.

In order to achieve the above object, in a first aspect, a shell assembly of an integrated machine for nucleic acid extraction and purification is provided, which comprises a shell, a working base, a temperature adjusting structure, a top seat and a control panel; the working base is arranged at the inner bottom of the shell, the temperature adjusting structure is arranged on at least two inner side walls of the shell, the temperature adjusting structure comprises heat insulating layers respectively clinging to the inner side walls, the top seat is arranged at the top of the shell, and the control panel is arranged in an installation groove formed in the front part of the upper end surface of the top seat; the working base, the temperature adjusting structure and the top base are enclosed to form a nucleic acid extraction working chamber; and a bin door is arranged on the front side surface of the shell.

Furthermore, the temperature adjusting structure also comprises side plates which are respectively parallel to the heat insulating layers, each side plate and the corresponding heat insulating layer respectively enclose a temperature adjusting chamber, and a plurality of air outlet holes are formed in each side plate; each of the tempering chambers is adapted to receive a cold source or a heat source from the outside.

Furthermore, the lower part of the temperature adjusting chamber is connected with one end of an air inlet pipe, the other end of the air inlet pipe is connected to one end of a second conveying pipe, the upper part of the temperature adjusting chamber is connected with one end of an exhaust pipe, the other end of the exhaust pipe is connected to one end of a first conveying pipe, and the other end of the first conveying pipe and the other end of the second conveying pipe are respectively connected to an external cold source or a heat source.

Further, the first delivery pipe and the second delivery pipe are both connected to an external cold source or heat source through electromagnetic valves.

Furthermore, a connecting plate is movably connected between the back surface of the control panel and the upper end surface of the shell, and the control panel is arranged in the mounting groove in a turnover mode through the connecting plate.

Furthermore, at least one ultraviolet disinfection lamp and at least one illuminating lamp are installed on the lower end face of the top seat.

Furthermore, the bottom surface of the shell is provided with a groove, and the left side and the right side of the top of the groove are respectively provided with a supporting seat.

Furthermore, a liquid accumulation groove is arranged in the edge area of the inner bottom surface of the shell and is connected with a liquid discharge pipe.

Furthermore, the upper end of the bin door is hinged to the front side face of the top seat, and the bin door is an anti-explosion glass door for isolating ultraviolet rays.

In a second aspect, a nucleic acid extraction and purification all-in-one machine is provided, which comprises the nucleic acid extraction and purification all-in-one machine housing assembly according to any one of the technical schemes of the first aspect.

Compared with the prior art, the beneficial effects of the utility model are that: through the temperature adjusting structure, on one hand, the heat preservation performance can be improved on the basis of the original heating function, the heating efficiency is improved, the heating time is shortened, and the heating temperature is stabilized, so that the efficiency and the quality of nucleic acid extraction are improved, and electricity can be saved; on the other hand, an external cold source or a heat source can be timely accessed when needed, wherein the cold source can be used for temporarily refrigerating trace nucleic acid obtained by extraction and purification, and a magnetic bead recovery step is also provided after the purification step of obtaining the nucleic acid is finished, so that the nucleic acid can be cooled and temporarily stored through the external cold source in the magnetic bead recovery process, and the activity of the nucleic acid can be favorably maintained; the heat source can combine to heat the sample in the deep hole board with original heating block, makes the heating more even, because the heating block only carries out local heating to the bottom of deep hole board, and intraductal sample is heated inhomogeneously, and other heat sources of outside introduction, like hot-air, can heat the deep hole board wholly, and inside sample can the thermally equivalent.

Drawings

Fig. 1 is a schematic front view of an embodiment of the present invention;

fig. 2 is a schematic top view of an embodiment of the present invention;

fig. 3 is a schematic view of the internal structure of the housing according to the embodiment of the present invention;

fig. 4 is a schematic view of a sectional structure of a housing according to an embodiment of the present invention.

In the figure: 1. a housing; 10. a nucleic acid extraction chamber; 11. a groove; 12. a supporting seat; 13. a liquid accumulation tank; 14. a liquid discharge pipe; 15. a bin gate; 2. a working base; 3. a temperature adjusting structure; 30. an exhaust pipe; 31. an air outlet; 32. a side plate; 33. a heat-insulating layer; 34. adjusting the temperature in the room; 35. an air inlet pipe; 36. a first delivery pipe; 37. a second delivery pipe; 4. a top seat; 40. an ultraviolet disinfection lamp; 41. an illuminating lamp; 42. mounting grooves; 5. a control panel; 50. a connecting plate.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings. It is to be understood that the embodiments described herein are merely some embodiments of the invention, and not all embodiments. Based on these embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.

As shown in fig. 1-4, an embodiment of the casing assembly of the integrated machine for nucleic acid extraction and purification of the present invention comprises a casing 1, a work base 2, a temperature adjustment structure 3, a top seat 4 and a control panel 5; the working base 2 is arranged at the inner bottom of the shell 1, the temperature adjusting structure 3 is arranged on at least two inner side walls of the shell 1, the temperature adjusting structure 3 comprises heat insulating layers 33 respectively clinging to the inner side walls, the top seat 4 is arranged at the top of the shell 1, and the control panel 5 is arranged in a mounting groove 45 formed in the front part of the upper end surface of the top seat 4; the working base 2, the temperature adjusting structure 3 and the top seat 4 are enclosed to form a nucleic acid extraction working chamber 10; a bin gate 15 is mounted on the front side of the housing 1. In one embodiment, as shown in fig. 4, the temperature conditioning structure 3 may be provided only on the left and right side walls of the housing 1. In other embodiments, the temperature adjusting structure 3 may be additionally provided on at least one of the rear side wall, the top wall and the bottom wall of the housing 1. In short, the larger the covered area, the better the tempering effect. The temperature regulation comprises heat preservation, temperature rise and temperature reduction. In one embodiment, the insulation layer 33 is formed by coating an insulation coating material, or is filled with insulation filler, or is formed by using a vacuum insulation chamber.

In one embodiment, as shown in fig. 4, the temperature adjusting structure 3 further includes side plates 32 parallel to the insulating layers 33, respectively, each of the side plates 32 and the corresponding insulating layer 33 enclose a temperature adjusting chamber 34, and a plurality of air outlet holes 31 are formed in each of the side plates 32; each of the temperature adjusting chambers 34 is adapted to receive a cold source or a hot source from the outside. The side plates 32 correspond to the heat insulating layers 33 one by one, and the edges of the side plates and the heat insulating layers are connected through a certain structure to form a surrounding structure, so that the temperature adjusting chamber 34 is formed. After entering the temperature adjusting chamber 34, an external cold source or a heat source enters the nucleic acid extraction working chamber 10 through the air outlet holes 31 on the side plates 32, wherein the heat source can uniformly heat the sample, and the cold source can temporarily store trace nucleic acid obtained by extraction and purification at a low temperature.

In one embodiment, as shown in fig. 2, the lower portion of the temperature-adjusting chamber 34 is connected to one end of an air inlet pipe 35, the other end of the air inlet pipe 35 is connected to one end of a second delivery pipe 37, the upper portion of the temperature-adjusting chamber 34 is connected to one end of an exhaust pipe 30, the other end of the exhaust pipe 30 is connected to one end of a first delivery pipe 36, and the other ends of the first delivery pipe 36 and the second delivery pipe 37 are respectively connected to an external cold source or a heat source. The pipelines form a heat circulation passage, which is equivalent to reserving an interface for connecting an external cold source or a heat source, can be randomly adapted to the external cold source or the external heat source according to the required cold quantity or heat quantity, and has convenient and flexible use and wide application range.

In one embodiment, the first delivery pipe 36 and the second delivery pipe 37 are connected to an external cold source or heat source through solenoid valves (not shown). The electromagnetic valve can realize the automatic opening and closing of the heat circulation passage. In actual use, a temperature sensor (not shown) may be disposed at a specific position in the nucleic acid extraction chamber 10, and the electromagnetic valve may be automatically controlled according to the temperature monitored in real time, which is convenient for use.

In one embodiment, as shown in fig. 2, a connecting plate 50 is movably connected between the back surface of the control panel 5 and the upper end surface of the housing 1, and the control panel 5 is installed in the installation groove 45 in a reversible manner through the connecting plate 50. The control panel 5 can turn the operation surface to face an operator when in use, and can turn the operation surface to be attached to the upper end surface of the shell 1 when not in use, thereby being beneficial to protecting the control panel 5 from being polluted by external complex biochemical environment and being prevented from being accidentally damaged by external collision.

In one embodiment, as shown in fig. 3, at least one ultraviolet sterilizing lamp 40 and at least one illuminating lamp 42 are installed on the lower end surface of the top chassis 4. The ultraviolet sterilizing lamp 40 can be turned on for sterilization when necessary, for example, the nucleic acid extracting chamber 10 can be sterilized for a certain period of time after the extraction operation is completed, so that it can be ensured that the sample is not contaminated when used next time. The illumination lamp 42 is turned on to facilitate the operator to observe the working process in the nucleic acid extraction chamber 10, which is advantageous for the operator to intervene in the nucleic acid extraction process manually when necessary, thereby preventing accidents.

In one embodiment, the bottom surface of the housing 1 is formed with a groove 11, and the left side and the right side of the top of the groove 11 are respectively provided with a support seat 12. The supporting seat 12 separates the middle of the bottom surface of the shell 1 from the experimental table top, so that the influence of the table top vibration on the inside of the shell 1 can be reduced.

In one embodiment, as shown in fig. 4, the inner bottom edge region of the housing 1 is provided with a liquid accumulation tank 13, and the liquid accumulation tank 13 is connected with a liquid discharge pipe 14. The liquid that accidentally spills in the virus extraction process is exported to casing 1 outside through fluid-discharge tube 14 after collecting through hydrops groove 13, is favorable to keeping the humidity environment of nucleic acid extraction studio 10 at design range.

In one embodiment, the upper end of the door 15 is hinged to the front side surface of the top seat 4, and the door 15 is an ultraviolet-proof explosion-proof glass door. Therefore, the bin door 15 can be turned over and opened conveniently. Can prevent the damage of ultraviolet rays to operators and prevent the internal explosion from damaging the bin gate 15 so as to cause virus leakage and harm to the operators.

The utility model relates to a control software adopts prior art.

Although a plurality of embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that a variety of changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A shell assembly of a nucleic acid extraction and purification all-in-one machine is characterized by comprising a shell (1), a working base (2), a temperature adjusting structure (3), a top seat (4) and a control panel (5); the working base (2) is arranged at the inner bottom of the shell (1), the temperature adjusting structure (3) is arranged on at least two inner side walls of the shell (1), the temperature adjusting structure (3) comprises heat insulating layers (33) tightly attached to the inner side walls respectively, the top seat (4) is arranged at the top of the shell (1), and the control panel (5) is arranged in a mounting groove (45) formed in the front part of the upper end face of the top seat (4); the working base (2), the temperature adjusting structure (3) and the top seat (4) are enclosed to form a nucleic acid extracting working chamber (10); and a bin door (15) is arranged on the front side surface of the shell (1).

2. The integrated nucleic acid extraction and purification machine shell assembly according to claim 1, wherein the temperature adjustment structure (3) further comprises side plates (32) parallel to the heat insulation layers (33), the side plates (32) and the corresponding heat insulation layers (33) enclose a temperature adjustment chamber (34), and a plurality of air outlet holes (31) are formed in each side plate (32); each of the temperature-controlled chambers (34) is adapted to receive a cold source or a heat source from the outside.

3. The integrated nucleic acid extracting and purifying machine shell assembly according to claim 2, wherein the lower portion of the temperature regulating chamber (34) is connected with one end of an air inlet pipe (35), the other end of the air inlet pipe (35) is connected with one end of a second conveying pipe (37), the upper portion of the temperature regulating chamber (34) is connected with one end of an exhaust pipe (30), the other end of the exhaust pipe (30) is connected with one end of a first conveying pipe (36), and the other ends of the first conveying pipe (36) and the second conveying pipe (37) are respectively connected with an external cold source or heat source.

4. The integrated nucleic acid extracting and purifying machine shell assembly according to claim 3, wherein the first delivery pipe (36) and the second delivery pipe (37) are connected to an external cold source or heat source through electromagnetic valves.

5. The integrated nucleic acid extracting and purifying machine shell assembly according to claim 1, wherein a connecting plate (50) is movably connected between the back surface of the control panel (5) and the upper end surface of the shell (1), and the control panel (5) is installed in the installation groove (45) in a reversible manner through the connecting plate (50).

6. The integrated nucleic acid extracting and purifying machine shell assembly according to claim 1, wherein the lower end face of the top seat (4) is provided with at least one ultraviolet disinfection lamp (40) and at least one illuminating lamp (42).

7. The integrated nucleic acid extraction and purification machine shell assembly according to claim 1, wherein a groove (11) is formed in the bottom surface of the shell (1), and a support seat (12) is respectively arranged on the left side and the right side of the top of the groove (11).

8. The integrated nucleic acid extraction and purification machine shell assembly according to claim 1, wherein a liquid accumulation tank (13) is arranged at the edge region of the inner bottom surface of the shell (1), and the liquid accumulation tank (13) is connected with a liquid discharge pipe (14).

9. The integrated nucleic acid extraction and purification machine shell assembly according to claim 1, wherein the upper end of the door (15) is hinged to the front side surface of the top seat (4), and the door (15) is an ultraviolet-proof explosion-proof glass door.

10. An integrated machine for nucleic acid extraction and purification, comprising the integrated machine for nucleic acid extraction and purification housing assembly of any one of claims 1-9.