CN215050308U - Sample pretreatment device for PCR (polymerase chain reaction) test - Google Patents
Sample pretreatment device for PCR (polymerase chain reaction) test Download PDFInfo
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- CN215050308U CN215050308U CN202120141111.XU CN202120141111U CN215050308U CN 215050308 U CN215050308 U CN 215050308U CN 202120141111 U CN202120141111 U CN 202120141111U CN 215050308 U CN215050308 U CN 215050308U
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Abstract
The utility model discloses a processing apparatus before sample that PCR test used, include: the device comprises a storage box, a rotating shaft, a rotating motor, a moving frame, a connecting rod, an electric telescopic rod, a driving motor, a driving rod and a placing groove; the storage box is of a cavity structure with an opening at the top, a rotating shaft is arranged in the storage box and connected with a rotating motor, a moving frame is arranged on the rotating shaft, and a plurality of connecting rods are arranged on the moving frame; connect electric telescopic handle on the preservation case lateral wall, electric telescopic handle is connected with driving motor, and electric telescopic handle is connected with the drive rod, and connecting rod, drive rod, preservation case lateral wall upper portion all are connected with the standing groove. The temperature control is conveniently carried out after the sample is centrifugally treated.
Description
Technical Field
The utility model relates to a preceding processing apparatus technical field, concretely relates to sample preceding processing apparatus of PCR experiment usefulness.
Background
In the PCR (polymerase chain reaction), DNA is denatured at 95 ℃ in vitro to become single-stranded, and at low temperature (usually about 60 ℃) the primer and single-stranded are combined according to the principle of base complementary pairing, and then the temperature is adjusted to the optimum reaction temperature (about 72 ℃) of DNA polymerase, and the DNA polymerase synthesizes a complementary strand along the direction from phosphate to pentose (5 '-3'). The PCR instrument manufactured based on polymerase is actually a temperature control device, and can be well controlled among denaturation temperature, renaturation temperature and extension temperature.
During PCR test, samples need to be processed by centrifugation, temperature control treatment and the like. Thus, a sample pretreatment device for PCR test is provided. The temperature control treatment is convenient to carry out during the test.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a processing apparatus before sample of PCR experiment usefulness, the problem of being difficult for carrying out temperature control after solving the sample and carrying out centrifugal treatment.
In order to solve the technical problem, the utility model adopts the following technical scheme:
a sample pretreatment device for PCR test, comprising: the device comprises a storage box, a rotating shaft, a rotating motor, a moving frame, a connecting rod, an electric telescopic rod, a driving motor, a driving rod and a placing groove;
the storage box is of a cavity structure with an opening at the top, a rotating shaft is arranged in the storage box and connected with a rotating motor, a moving frame is arranged on the rotating shaft, and a plurality of connecting rods are arranged on the moving frame;
connect electric telescopic handle on the preservation case lateral wall, electric telescopic handle is connected with driving motor, and electric telescopic handle is connected with the drive rod, and connecting rod, drive rod, preservation case lateral wall upper portion all are connected with the standing groove.
Further, the method comprises the following steps of; the top of the storage box is movably connected with a top cover, and a sealing ring is arranged on the side wall of the top cover.
Further, the method comprises the following steps of; the side wall of the preservation box is connected with a treatment box, and one side of the treatment box is uniformly provided with air holes;
two layers of filter screen plates are arranged in the treatment box, and a mixture of a drying agent and an adsorbent is filled between the filter screen plates;
the side wall of the storage box is of a cavity structure, the side wall of the storage box cavity is communicated with the processing box through a first air pipe, and an air cooler is arranged on the first air pipe.
Further, the method comprises the following steps of; the side wall of the cavity of the storage box is connected with a second air pipe, and a first electromagnetic valve is arranged on the second air pipe.
Further, the method comprises the following steps of; the preservation box and the processing box are communicated through a third air pipe, the preservation box is connected with a fourth air pipe, a second electromagnetic valve is arranged on the fourth air pipe, and power pumps are arranged on the first air pipe, the third air pipe and the fourth air pipe.
Further, the method comprises the following steps of; a temperature sensor is arranged in the storage box, and the temperature sensor, the air cooler, the power pump on the first air pipe and the first electromagnetic valve are all connected with a first controller.
Further, the method comprises the following steps of; and a humidity sensor is arranged in the storage box, and the humidity sensor, the power pump on the third air pipe, the power pump on the fourth air pipe and the second electromagnetic valve are connected with a second controller.
Compared with the prior art, the utility model discloses an at least, have one of following beneficial effect:
1. the sample of storage such as test tube need carry out the processing that keeps warm at the uniform temperature, the sample of test tube can be put and is taken the pole, keep warm in the standing groove on the storage box lateral wall and handle, it drives the motion at electric telescopic handle as required to drive the pole, the placing of the test tube of being convenient for etc., the pivot drives the connecting rod motion of moving the connection of putting up, the standing groove on the connecting rod also moves, test tube etc. in the standing groove of the connecting rod department of being convenient for play the uniform motion about, the different sample's of being convenient for needs.
2. The top cover moves as required, and is connected to the storage box through the locking structure.
Drawings
Fig. 1 is a schematic structural diagram of the present invention.
In the figure: the device comprises a storage box (1), a rotating shaft (2), a rotating motor (3), a moving frame (4), a connecting rod (5), an electric telescopic rod (6), a driving motor (7), a driving rod (8), a placing groove (9), a top cover (10), a sealing ring (11), a processing box (12), a filter screen plate (13), a first air pipe (14), an air cooler (15), a second air pipe (16), a first electromagnetic valve (17), a third air pipe (18), a fourth air pipe (19), a second electromagnetic valve (20), a power pump (21), a temperature sensor (22), a first controller (23), a humidity sensor (24) and a second controller (25).
Detailed Description
Fig. 1 is a schematic diagram illustrating an embodiment of the present invention, and the following description is provided with reference to the accompanying drawings. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Example 1:
a sample pretreatment device for PCR test, comprising: the device comprises a storage box 1, a rotating shaft 2, a rotating motor 3, a moving frame 4, a connecting rod 5, an electric telescopic rod 6, a driving motor 7, a driving rod 8 and a placing groove 9;
the storage box 1 is of a cavity structure with an opening at the top, a rotating shaft 2 is arranged in the storage box 1, the rotating shaft 2 is connected with a rotating motor 3, a moving frame 4 is arranged on the rotating shaft 2, and a plurality of connecting rods 5 are arranged on the moving frame 4;
the side wall of the storage box 1 is connected with an electric telescopic rod 6, the electric telescopic rod 6 is connected with a driving motor 7, the electric telescopic rod 6 is connected with a driving rod 8, and the connecting rod 5, the driving rod 8 and the upper part of the side wall of the storage box 1 are connected with a placing groove 9; the sample of storage such as test tube needs carry out the processing that keeps warm under the uniform temperature, the sample of test tube can be put and is taken action bar 8, the processing that keeps warm in standing groove 9 on 1 lateral wall of storage box, it moves at electric telescopic handle 6 drive as required to take action bar 8, the placing of the test tube of being convenient for etc., pivot 2 drives the 5 movements of connecting rod that remove 4 connections, standing groove 9 also moves on the connecting rod 5, the test tube etc. in the standing groove 9 of the connecting rod 5 department of being convenient for play certain motion about, the needs of the different samples of being convenient for.
Example 2:
on the basis of embodiment 1, the top of the storage box 1 is movably connected with a top cover 10, and a sealing ring 11 is arranged on the side wall of the top cover 10; the movement of the top cover 10 is performed as required, and the top cover 10 is coupled to the storage case 1 by a locker structure.
Example 3:
on the basis of the embodiment 1-2, the side wall of the preservation box 1 is connected with a processing box 12, and one side of the processing box 12 is uniformly provided with air holes;
two layers of filter screen plates 13 are arranged in the treatment box 12, and a mixture of a drying agent and an adsorbent is filled between the filter screen plates 13;
the side wall of the preservation box 1 is a cavity structure, the side wall of the cavity of the preservation box 1 is communicated with the treatment box 12 through a first air pipe 14, and an air cooler 15 is arranged on the first air pipe 14; the air entering the treatment box 12 passes through the filter screen 13 and is filled with the mixture of the drying agent and the adsorbent, so that the drying box can adsorb the air, and when the preservation box 1 needs to preserve at a certain temperature, the preservation box 1 enters the air cooler 15 to cool the air into the side wall of the cavity of the preservation box 1.
Example 4:
on the basis of the embodiment 1-3, the side wall of the cavity of the storage box 1 is connected with a second air pipe 16, and the second air pipe 16 is provided with a first electromagnetic valve 17; the air in the side wall of the cavity of the storage box 1 can be discharged through the second air pipe 16.
Example 5:
on the basis of the embodiments 1-4, the storage box 1 and the treatment box 12 are communicated through a third air pipe 18, the storage box 1 is connected with a fourth air pipe 19, the fourth air pipe 19 is provided with a second electromagnetic valve 20, and the first air pipe 14, the third air pipe 18 and the fourth air pipe 19 are all provided with a power pump 21; the inside of the storage box 1 may be introduced with dry air through the third air duct 18.
Example 6:
on the basis of the embodiments 1-5, the temperature sensor 22 is arranged in the storage box 1, and the temperature sensor 22, the air cooler 15, the power pump 21 on the first air pipe 14 and the first electromagnetic valve 17 are all connected with the first controller 23; the temperature monitoring is convenient.
Example 7:
on the basis of the embodiments 1 to 6, a humidity sensor 24 is arranged in the storage box 1, and the humidity sensor 24, the power pump 21 on the third air pipe 18, the power pump 21 on the fourth air pipe 19 and the second electromagnetic valve 20 are all connected with a second controller 25; the humidity monitoring is convenient.
Although the invention has been described herein with reference to a number of illustrative embodiments thereof, it should be understood that numerous other modifications and embodiments can be devised by those skilled in the art that will fall within the spirit and scope of the principles of this invention. More specifically, various variations and modifications are possible in the component parts and/or arrangements of the subject combination arrangement within the scope of the disclosure, the drawings and the appended claims. In addition to variations and modifications in the component parts and/or arrangements, other uses will also be apparent to those skilled in the art.
Claims (7)
1. A sample pretreatment device for PCR tests is characterized in that: the method comprises the following steps: the device comprises a storage box (1), a rotating shaft (2), a rotating motor (3), a moving frame (4), a connecting rod (5), an electric telescopic rod (6), a driving motor (7), a driving rod (8) and a placing groove (9);
the storage box (1) is of a cavity structure with an opening at the top, a rotating shaft (2) is arranged in the storage box (1), the rotating shaft (2) is connected with a rotating motor (3), a moving frame (4) is arranged on the rotating shaft (2), and a plurality of connecting rods (5) are arranged on the moving frame (4);
electric telescopic handle (6) are connected on the lateral wall of storage box (1), electric telescopic handle (6) are connected with driving motor (7), electric telescopic handle (6) are connected with drive rod (8), connecting rod (5), drive rod (8), storage box (1) lateral wall upper portion all is connected with standing groove (9).
2. The apparatus for pretreating a sample for a PCR test according to claim 1, wherein: the top of the storage box (1) is movably connected with a top cover (10), and a sealing ring (11) is arranged on the side wall of the top cover (10).
3. The apparatus for pretreating a sample for a PCR test according to claim 1, wherein: the side wall of the storage box (1) is connected with a processing box (12), and air holes are uniformly formed in one side of the processing box (12);
two layers of filter screen plates (13) are arranged in the treatment box (12), and a mixture of a drying agent and an adsorbent is filled between the filter screen plates (13);
the side wall of the storage box (1) is of a cavity structure, the side wall of the cavity of the storage box (1) is communicated with the processing box (12) through a first air pipe (14), and an air cooler (15) is arranged on the first air pipe (14).
4. The apparatus for pretreating a sample for a PCR test according to claim 1, wherein: the side wall of the cavity of the storage box (1) is connected with a second air pipe (16), and a first electromagnetic valve (17) is arranged on the second air pipe (16).
5. The apparatus for pretreating a sample for a PCR test according to claim 1, wherein: the storage box (1) and the treatment box (12) are communicated through a third air pipe (18), the storage box (1) is connected with a fourth air pipe (19), a second electromagnetic valve (20) is arranged on the fourth air pipe (19), and power pumps (21) are arranged on the first air pipe (14), the third air pipe (18) and the fourth air pipe (19).
6. The apparatus for pretreating a sample for a PCR test according to claim 1, wherein: a temperature sensor (22) is arranged in the storage box (1), and the temperature sensor (22), the air cooler (15), the power pump (21) on the first air pipe (14) and the first electromagnetic valve (17) are connected with a first controller (23).
7. The apparatus for pretreating a sample for a PCR test according to claim 1, wherein: a humidity sensor (24) is arranged in the storage box (1), and the humidity sensor (24), a power pump (21) on the third air pipe (18), the power pump (21) on the fourth air pipe (19) and the second electromagnetic valve (20) are connected with a second controller (25).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN202120141111.XU CN215050308U (en) | 2021-01-19 | 2021-01-19 | Sample pretreatment device for PCR (polymerase chain reaction) test |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN202120141111.XU CN215050308U (en) | 2021-01-19 | 2021-01-19 | Sample pretreatment device for PCR (polymerase chain reaction) test |
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Publication Number | Publication Date |
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CN215050308U true CN215050308U (en) | 2021-12-07 |
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CN202120141111.XU Active CN215050308U (en) | 2021-01-19 | 2021-01-19 | Sample pretreatment device for PCR (polymerase chain reaction) test |
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CN (1) | CN215050308U (en) |
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2021
- 2021-01-19 CN CN202120141111.XU patent/CN215050308U/en active Active
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