CN215044975U

CN215044975U - ELISA kit for detecting fipronil

Info

Publication number: CN215044975U
Application number: CN202120828447.3U
Authority: CN
Inventors: 孙杰超; 于兴华; 葛怀娜; 遇靓; 郭志雄; 孙惠洁
Original assignee: Xinda An Testing Technology Tianjin Co ltd
Current assignee: Xinda An Testing Technology Tianjin Co ltd
Priority date: 2021-04-21
Filing date: 2021-04-21
Publication date: 2021-12-07
Anticipated expiration: 2031-04-21

Abstract

The utility model provides an ELISA kit for detecting fipronil, which comprises a box body, a cover plate matched with the box body, an ELISA plate embedded in the box body, a stable component and an ejection component, wherein the stable component and the ejection component are arranged in the box body; a positioning groove is formed in the box body and used for fixing the enzyme label plate; the stabilizing assembly comprises a cavity arranged in the box body, a through groove communicated with the cavity and a stabilizing plate connected in the cavity through the sliding assembly, and the stabilizing plate is matched with the through groove; the ejection assembly is used for ejecting the ELISA plate from the positioning groove. The utility model relates to a detect ELISA kit of fipronil, this kit include firm subassembly, firm subassembly includes the steadying plate, can further stabilize the ELIAS plate in the constant head tank, maintains the stability of ELIAS plate when being convenient for the test, prevents that ELIAS plate and inside mixed liquid from rocking and spilling outside the ELIAS plate, causes the waste of reagent.

Description

ELISA kit for detecting fipronil

Technical Field

The utility model relates to a detect ELISA kit of fipronil belongs to kit technical field.

Background

Fipronil as a broad-spectrum pesticide has the insecticidal action on aphids, plant hoppers, lepidoptera larvae and other pests by blocking the chloride metabolism of the pests, the kit is widely applied to insect pest prevention of vegetables, a commonly used kit for detecting fipronil is an ELISA kit, a proper amount of standard solution/sample, antibody mixed solution and reaction substrate solution are sequentially added into an ELISA plate with 6 holes or 8 holes for reaction, then an ELISA reader is used for detecting the wavelength of reaction solution for detecting the concentration of the fipronil, however, the existing common ELISA kit for detecting fipronil is very expensive, especially in the process of mixing reaction of a sample and an antibody, the ELISA plate needs to be stabilized so as to avoid the rupture of the ELISA plate or the spill of reaction liquid and avoid the overhigh detection cost of the fipronil, therefore, it is necessary to develop an ELISA kit for detecting fipronil, which can stabilize the ELISA plate.

SUMMERY OF THE UTILITY MODEL

The utility model aims to overcome the defects in the prior art and provide an ELISA kit for detecting fipronil, which solves the problems in the prior art.

In order to achieve the above object, the present invention is achieved by the following technical solutions, including:

an ELISA kit for detecting fipronil comprises a box body, a cover plate matched with the box body, an ELISA plate embedded in the box body, a stable component and an ejection component, wherein the stable component and the ejection component are arranged in the box body; a positioning groove is formed in the box body and used for fixing the enzyme label plate; the stabilizing assembly comprises a cavity arranged in the box body, a through groove communicated with the cavity and a stabilizing plate connected in the cavity through the sliding assembly, and the stabilizing plate is matched with the through groove; the ejection assembly is used for ejecting the ELISA plate from the positioning groove.

Preferably, the surface of the stabilizing plate facing the ELISA plate is integrally provided with a clamping block, and the corresponding surface of the ELISA plate is provided with a clamping groove.

Preferably, the sliding assembly comprises an elastic element and a limiting column, two ends of the elastic element are respectively and fixedly connected with the inner wall of the cavity and the stabilizing plate, and one end of the limiting column is fixed on the inner wall of the cavity and the other end of the limiting column is suspended in the elastic element.

Preferably, pop out the subassembly and include reset push rod and connecting rod, connecting rod and steadying plate fixed connection, reset push rod one end is extended to the box body outside through first through-hole and the other end articulates there is flexible transfer line, and flexible transfer line middle part articulates in the cavity and both ends are articulated mutually with reset push rod and connecting rod respectively.

Preferably, two limiting blocks are fixed in the cavity, the reset push rod and the connecting rod are sleeved in the limiting blocks, and the limiting blocks are used for enabling the reset push rod and the connecting rod to keep moving in the horizontal direction.

Preferably, the end part of the reset push rod extending to the outer side of the box body is fixed with a reset button, and the radius of the reset button is larger than that of the first through hole.

Preferably, the box body is provided with a groove, the groove is matched with the reset button, and a reset spring is fixed between the reset button and the groove.

Preferably, the ejection assembly comprises an extension rack integrally fixed at the end part of the reset push rod, a gear fixed in the cavity and meshed with the extension rack, an ejection rack meshed with the gear, and an ejection block fixedly connected with the ejection rack, wherein the ejection block is abutted to the elisa plate.

Preferably, the box body is provided with a second through hole, the second through hole is communicated with the cavity, and the pop-up block is matched with the second through hole.

Preferably, the box body is hinged with the cover plate.

The utility model has the advantages that:

(1) the utility model relates to a detect ELISA kit of fipronil, this kit include firm subassembly, firm subassembly includes the steadying plate, can further stabilize the ELIAS plate in the constant head tank, maintains the stability of ELIAS plate when being convenient for the test, prevents that ELIAS plate and inside mixed liquid from rocking and spilling outside the ELIAS plate, causes the waste of reagent.

(2) The utility model relates to a detect ELISA kit of fipronil, this kit is including popping out the subassembly, pop out the subassembly including popping out the piece for pop out the constant head tank with the ELIAS plate after the detection, play the effect of dismantling the kit fast.

Drawings

Other features, objects and advantages of the invention will become more apparent upon reading of the detailed description of non-limiting embodiments with reference to the following drawings:

fig. 1 is a schematic structural diagram of the kit of the present invention;

fig. 2 is an enlarged schematic view of a point a in fig. 1 according to the present invention.

In the figure: the automatic stamping device comprises a box body 1, a cover plate 2, an enzyme labeling plate 3, a positioning groove 4, a cavity 5, a through groove 6, a stabilizing plate 7, a clamping block 8, a clamping groove 9, an elastic element 10, a limiting column 11, a reset push rod 12, a connecting rod 13, a telescopic transmission rod 14, a first through hole 15, a limiting block 16, a reset button 17, a groove 18, a reset spring 19, an extension rack 20, a gear 21, an ejection rack 22, an ejection block 23 and a second through hole 24.

Detailed Description

In order to make the technical means, creation features, achievement purposes and functions of the present invention easy to understand, the present invention is further described below with reference to the following embodiments.

Example 1

As shown in figure 1, an ELISA kit for detecting fipronil comprises a box body 1, a cover plate 2 matched with the box body 1, an ELISA plate 3 embedded in the box body 1, a stable component arranged in the box body 1 and an ejection component; a positioning groove 4 is formed in the box body 1, and the positioning groove 4 is used for fixing the ELISA plate 3; the stabilizing assembly comprises a cavity 5 arranged in the box body 1, a through groove 6 communicated with the cavity 5 and a stabilizing plate 7 connected in the cavity 5 through a sliding assembly, and the stabilizing plate 7 is matched with the through groove 6; the ejection assembly is used for ejecting the microplate 3 from the positioning groove 4.

Referring to fig. 2, a clamping block 8 is integrally arranged on the surface of the stabilizing plate 7 facing the elisa plate 3, and a clamping groove 9 is formed on the corresponding surface of the elisa plate 3.

The sliding assembly comprises an elastic element 10 and a limiting column 11, two ends of the elastic element 10 are fixedly connected with the inner wall of the cavity 5 and the stabilizing plate 7 respectively, one end of the limiting column 11 is fixed on the inner wall of the cavity 5, the other end of the limiting column is suspended in the elastic element 10, the elastic element 10 can be a spring in the embodiment, and the elastic element 10 enables the fixture block 8 to extend to the outer side of the through groove 6 under the condition of no stress.

The pop-up assembly comprises a reset push rod 12 and a connecting rod 13, the connecting rod 13 is fixedly connected with the stabilizing plate 7, one end of the reset push rod 12 extends to the outer side of the box body 1 through a first through hole 15, the other end of the reset push rod is hinged with a telescopic transmission rod 14, the middle part of the telescopic transmission rod 14 is hinged in the cavity 5, and two ends of the telescopic transmission rod are hinged with the reset push rod 12 and the connecting rod 13 respectively.

Two limiting blocks 16 are fixed in the cavity 5, the reset push rod 12 and the connecting rod 13 are sleeved in the limiting blocks 16, and the limiting blocks 16 are used for enabling the reset push rod 12 and the connecting rod 13 to keep moving in the horizontal direction.

The end part of the reset push rod 12 extending to the outer side of the box body 1 is fixed with a reset button 17, and the radius of the reset button 17 is larger than that of the first through hole 15.

The box body 1 is provided with a groove 18, the groove 18 is matched with a reset button 17, and a reset spring 19 is fixed between the reset button 17 and the groove 18.

The ejection assembly comprises an extension rack 20 integrally fixed at the end part of the reset push rod 12, a gear 21 fixed in the cavity 5 and meshed with the extension rack 20, an ejection rack 22 meshed with the gear 21, and an ejection block 23 fixedly connected with the ejection rack 22, wherein the ejection block 23 is abutted against the microplate 3.

The box body 1 is provided with a second through hole 24, the second through hole 24 is communicated with the cavity 5, and the pop-up block 23 is matched with the second through hole 24.

The box body 1 is hinged with the cover plate 2.

The utility model discloses a theory of operation and operation process: when the fipronil is detected, the elisa plate 3 is placed into the positioning groove 4, the side wall of the elisa plate 3 extrudes the fixture block 8 downwards, the fixture block 8 is stressed to compress the elastic element 10, when the elisa plate 3 is placed to the bottom of the positioning groove 4, the position of the clamping groove 9 corresponds to the fixture block 8 right, the elastic element 10 recovers elasticity, the fixture block 8 is embedded into the clamping groove 9, the stable clamping of the elisa plate 3 is enabled to be in the positioning groove 4, after the fipronil is detected, the reset button 17 is pressed inwards, the reset button 17 drives the reset push rod 12 to move inwards, the extension rack 20 moves forwards, the gear 21 meshed with the extension rack is driven to rotate, the gear 21 rotates to drive the ejection rack 22 to move upwards, the ejection block 23 ejects the elisa plate 3 upwards, and an operator can take out the elisa plate 3 conveniently.

The basic principles and the main features of the invention and the advantages of the invention have been shown and described above, it will be evident to those skilled in the art that the invention is not limited to the details of the foregoing illustrative embodiments, but that the invention may be embodied in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. An ELISA kit for detecting fipronil, which is characterized in that:

comprises a box body (1), a cover plate (2) matched with the box body (1), an enzyme label plate (3) embedded in the box body (1), a stable component arranged in the box body (1) and an ejection component;

a positioning groove (4) is formed in the box body (1), and the positioning groove (4) is used for fixing the enzyme label plate (3);

the stabilizing assembly comprises a cavity (5) arranged in the box body (1), a through groove (6) communicated with the cavity (5) and a stabilizing plate (7) connected in the cavity (5) through a sliding assembly, and the stabilizing plate (7) is matched with the through groove (6);

the ejection assembly is used for ejecting the ELISA plate (3) from the positioning groove (4).

2. The ELISA kit for detecting fipronil according to claim 1, wherein: the surface of the stabilizing plate (7) facing the ELISA plate (3) is integrally provided with a clamping block (8), and the corresponding surface of the ELISA plate (3) is provided with a clamping groove (9).

3. The ELISA kit for detecting fipronil according to claim 1, wherein: the sliding assembly comprises an elastic element (10) and a limiting column (11), two ends of the elastic element (10) are fixedly connected with the inner wall of the cavity (5) and the stabilizing plate (7) respectively, and one end of the limiting column (11) is fixed on the inner wall of the cavity (5) and the other end of the limiting column is suspended in the elastic element (10).

4. The ELISA kit for detecting fipronil according to claim 2 or 3, wherein: the pop-up assembly comprises a reset push rod (12) and a connecting rod (13), the connecting rod (13) is fixedly connected with the stabilizing plate (7), one end of the reset push rod (12) extends to the outer side of the box body (1) through a first through hole (15), the other end of the reset push rod is hinged with a telescopic transmission rod (14), the middle of the telescopic transmission rod (14) is hinged in the cavity (5), and the two ends of the telescopic transmission rod are hinged with the reset push rod (12) and the connecting rod (13) respectively.

5. The ELISA kit for detecting fipronil according to claim 4, wherein: two limiting blocks (16) are fixed in the cavity (5), the reset push rod (12) and the connecting rod (13) are sleeved in the limiting blocks (16), and the limiting blocks (16) are used for enabling the reset push rod (12) and the connecting rod (13) to keep moving in the horizontal direction.

6. The ELISA kit for detecting fipronil according to claim 5, wherein: the end part of the reset push rod (12) extending to the outer side of the box body (1) is fixedly provided with a reset button (17), and the radius of the reset button (17) is larger than that of the first through hole (15).

7. The ELISA kit for detecting fipronil according to claim 6, wherein: the box body (1) is provided with a groove (18), the groove (18) is matched with the reset button (17), and a reset spring (19) is fixed between the reset button (17) and the groove (18).

8. The ELISA kit for detecting fipronil according to claim 7, wherein: the ejection assembly comprises an extension rack (20) integrally fixed at the end part of the reset push rod (12), a gear (21) fixed in the cavity (5) and meshed with the extension rack (20), an ejection rack (22) meshed with the gear (21), and an ejection block (23) fixedly connected with the ejection rack (22), wherein the ejection block (23) is abutted to the elisa plate (3).

9. The ELISA kit for detecting fipronil according to claim 8, wherein: the box body (1) is provided with a second through hole (24), the second through hole (24) is communicated with the cavity (5), and the pop-up block (23) is matched with the second through hole (24).

10. The ELISA kit for detecting fipronil according to claim 9, wherein: the box body (1) is hinged with the cover plate (2).