CN214916209U - Centrifugal microfluidic device for rapidly preparing bi-component gel microspheres - Google Patents
Centrifugal microfluidic device for rapidly preparing bi-component gel microspheres Download PDFInfo
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Abstract
The utility model discloses a centrifugal microfluidic device for rapidly preparing bi-component gel microspheres, which belongs to the technical field of biomedical materials and comprises an inner liquid storage barrel and an outer liquid storage barrel, wherein the inner liquid storage barrel is coaxially arranged in the inner cavity of the outer liquid storage barrel, the bottom end of the inner cavity of the outer liquid storage barrel is provided with an annular boss extending to the top of the outer liquid storage barrel, and the bottom end of the inner liquid storage barrel is fixedly connected between the inner ring walls of the annular boss; a collecting tank with an opening at the top is arranged between the annular boss and the inner barrel wall of the outer liquid storage barrel, and collecting liquid is injected into the collecting tank; the inner liquid storage barrel is provided with double-layer liquid storage areas which are separated from each other so as to respectively inject precursor solutions with two different components; the outside of interior liquid storage bucket is equipped with a microchannel mechanism, and one side of microchannel mechanism has two links that are linked together with two-layer stock solution district one-to-one, and the opposite side of microchannel mechanism has collects the end, collects the top that the end is located the collecting vat. The utility model has the advantages of the liquid drop homogeneity that generates is high and can the high flux production bi-component gel microballon.
Description
Technical Field
The utility model relates to a biomedical materials technical field especially relates to a centrifugal micro-fluidic device is prepared fast to bi-component gel microballon.
Background
The hydrogel has the characteristics of both solid and liquid, and is suitable for serving as a biological lubricant, a drug delivery carrier, a cell culture scaffold and the like. Compared with the massive hydrogel, the gel microspheres have better dispersibility, larger specific surface area and faster response in a solution; compared with the solid microspheres, the gel microspheres are generally composed of polymer molecules, have high water content, are closer to the extracellular matrix environment, and have diversity and controllability of physical and chemical properties. The two-component gel microspheres including Janus microspheres and core-shell microspheres can load two components with different physical or chemical properties, so that the two-component gel microspheres have attracted extensive attention in the fields of tissue engineering, drug release, biological probes, regenerative medicine and the like.
The basic principle of the centrifugal microfluidic technology is that a micro motor drives a microfluidic platform to perform high-speed rotary motion to construct a simulated gravity field, namely a centrifugal field, so that the manipulation of various fluids with large differences in density, viscosity and surface tension is realized. Compared with the traditional micro-fluidic method based on the shearing force, the micro-fluidic method based on the centrifugal force generates liquid drops by the centrifugal force without being influenced by the flowing condition of continuous fluid, so that the micro-fluidic method can be a universal platform for generating polymer microgel and inorganic particles with adjustable size and structure. However, most of the existing centrifugal microfluidic platforms adopt a disc or tube chip design, and the number of flow channels is limited, which is insufficient for high-throughput microgel generation. Furthermore, direct parallelization of these centrifugal microfluidic devices has certain difficulties in both manufacturing complexity and system control.
SUMMERY OF THE UTILITY MODEL
The utility model aims at solving the problem mentioned in the background art, provide a centrifugal micro-fluidic device is prepared fast to bi-component gel microballon, the device has the advantage that can realize the high flux production of bi-component gel microballon when guaranteeing the microgel homogeneity that prepares.
In order to realize the technical purpose, the utility model discloses the technical scheme who takes does:
the utility model firstly provides a centrifugal microfluidic device for rapidly preparing bi-component gel microspheres, which comprises an inner liquid storage barrel and an outer liquid storage barrel, wherein the inner liquid storage barrel is coaxially arranged in the inner cavity of the outer liquid storage barrel, the bottom end of the inner cavity of the outer liquid storage barrel is provided with an annular boss extending to the top of the inner cavity, and the bottom end of the inner liquid storage barrel is fixedly connected between the inner annular walls of the annular boss; a collecting tank with an opening at the top is arranged between the annular boss and the inner barrel wall of the outer liquid storage barrel, and collecting liquid is injected into the collecting tank; the inner liquid storage barrel is provided with double-layer liquid storage areas which are separated from each other so as to respectively inject precursor solutions with two different components; the outer side of the inner liquid storage barrel is provided with a micro-channel mechanism, one side of the micro-channel mechanism is provided with two connecting ends which are communicated with the two liquid storage areas in a one-to-one correspondence manner, the other side of the micro-channel mechanism is provided with a collecting end, and the collecting end is positioned above the collecting groove;
still include a centrifugal drive mechanism, centrifugal drive mechanism is connected with outer liquid storage bucket, and it can drive outer liquid storage bucket and interior liquid storage bucket and rotate at high speed in step and further make the precursor solution in two-layer liquid storage district be centrifugal motion in interior liquid storage bucket, two the centrifugal force that precursor solution received when doing centrifugal motion can extrude the two and enter into the microchannel mechanism through the link that corresponds respectively in to end collection at microchannel mechanism collects and forms the liquid droplet, and the liquid droplet of formation can fall into in the collecting fluid in the collecting vat under the inertial action.
With regard to the above device, the centrifugal transmission mechanism includes a motor connecting sleeve, an upper connecting disc is arranged at the top of the motor connecting sleeve, the bottom end of the outer liquid storage barrel is fixedly connected with the upper connecting disc, a longitudinal through hole is arranged at the bottom of the motor connecting sleeve, and a motor shaft of the speed regulating motor is fixedly arranged in the longitudinal through hole, so that the speed regulating motor can drive the outer liquid storage barrel and the inner liquid storage barrel to synchronously rotate at high speed through the motor connecting sleeve.
Furthermore, a horizontal partition plate is arranged in the inner cavity of the barrel body of the inner liquid storage barrel, and a lower-layer liquid storage area is defined between the bottom of the partition plate and the inner wall of the barrel body of the inner liquid storage barrel; the top of interior liquid storage bucket is equipped with and extends and run through the transfusion hole to the baffle bottom towards its staving inner chamber, the top edge of transfusion hole is equipped with ascending tubulose boss, the top height of tubulose boss is less than the inner chamber top of interior liquid storage bucket, enclose into upper reservoir district between the top of baffle and the outer wall of tubulose boss and the interior bucket wall of interior liquid storage bucket.
Furthermore, a nested liquid storage barrel is coaxially arranged in the inner cavity of the inner liquid storage barrel, and the inner cavity of the nested liquid storage barrel is an inner-layer liquid storage area; the bottom end of the nested liquid storage barrel is fixed on the bottom surface of the inner cavity of the inner liquid storage barrel in a sealing mode, an outer liquid storage area is defined between the outer wall of the nested liquid storage barrel and the inner wall of the inner liquid storage barrel, and a liquid inlet hole penetrating into the inner cavity of the nested liquid storage barrel is formed in the top of the inner liquid storage barrel.
Furthermore, the micro-channel mechanism comprises an upper capillary and a lower capillary which are arranged in parallel, a bifurcation end and a gathering end are arranged between the upper capillary and the lower capillary, the orifice of the upper capillary and the orifice of the lower capillary at the bifurcation end are respectively and correspondingly connected to the upper-layer liquid storage area and the lower-layer liquid storage area, a gathering capillary is arranged on the outer side of the gathering end, and precursor solution flowing out of the upper capillary and the lower capillary can be gathered in the gathering capillary to flow out to form liquid drops.
Furthermore, the micro-channel mechanism comprises an inner phase capillary tube and an outer phase capillary tube, one end of the outer phase capillary tube is communicated with the outer layer liquid storage area, and the other end of the outer phase capillary tube extends out of the inner liquid storage barrel; one end of the inner phase capillary tube is communicated with the inner liquid storage area, the other end of the inner phase capillary tube is coaxially arranged in the outer phase capillary tube, and precursor solution flowing out of the inner phase capillary tube and the outer phase capillary tube can be collected and flow out at a port of the outer phase capillary tube, which extends out of the inner liquid storage barrel, so as to form liquid drops.
Furthermore, the microchannel mechanisms are arranged into a plurality of groups, and the plurality of groups of microchannel mechanisms are equally and angularly arranged on the outer side of the inner liquid storage barrel.
The utility model has the advantages of it is following:
1. the utility model can flexibly control the size and shape of the gel microsphere by adjusting the parameters of the motor such as centrifugal speed, capillary diameter and the like, and realize high-flux production of the gel microsphere while ensuring uniformity; meanwhile, the device is simple to build, the obtained gel microspheres do not need to be cleaned or only need to be cleaned simply, and subsequent treatment procedures are saved.
2. The utility model discloses a two kinds of component precursor solution are stored in the layering in the inner liquid storage bucket, utilize a plurality of capillary to be connected to the stock solution district that corresponds, gather together the end at above-mentioned a plurality of capillaries simultaneously and form and collect the department, and then under the effect of centrifugal force, the precursor solution in a plurality of capillaries can be in collecting the department and mix and form the bi-component liquid drop, and then can prepare the bi-component gel microballon.
3. The inner liquid storage barrel and the outer liquid storage barrel of the utility model are coaxially arranged, which can ensure the uniformity of the generated liquid drops; meanwhile, the inner liquid storage barrel is fixedly connected with the outer liquid storage barrel through threads, when the speed regulating motor drives the outer liquid storage barrel to rotate at a high speed, the hydrogel precursor solution, the oil phase and the collecting liquid are relatively static under the condition of high-speed rotation, and the bi-component gel microspheres with high sphericity can be generated in a large response range.
Drawings
Fig. 1 is a schematic view of the overall structure in a first embodiment of the present invention;
fig. 2 is a schematic view of the overall structure in the second embodiment of the present invention.
The label names in the figure: the device comprises a double-layer liquid storage barrel 1, an external thread section 1-1, an upper layer liquid storage area 1-2, a lower layer liquid storage area 1-3, an upper capillary 1-4, a lower capillary 1-5, a collecting capillary 1-6, an external liquid storage barrel 2, an internal thread section 2-1, a screw groove hole 2-2, a motor connecting sleeve 3, a longitudinal through hole 3-1, an upper connecting disc 3-2, a nut groove hole 3-3, an embedded liquid storage barrel 4, an external layer liquid storage area 4-1, an internal layer liquid storage area 4-2, an external phase capillary 4-3 and an internal phase capillary 4-4.
Detailed Description
Embodiments of the present invention will be described in further detail with reference to the accompanying drawings, and it is to be understood that the described embodiments are merely illustrative of some, but not all embodiments of the invention.
The first embodiment:
as shown in fig. 1, in this embodiment, a centrifugal microfluidic device is rapidly prepared from two-component gel microspheres, and includes an inner liquid storage barrel and an outer liquid storage barrel 2, wherein the inner liquid storage barrel is coaxially installed in an inner cavity of the outer liquid storage barrel 2, an annular boss extending to the top of the inner cavity of the outer liquid storage barrel 2 is arranged at the bottom end of the inner cavity of the outer liquid storage barrel, and the bottom end of the inner liquid storage barrel is fixedly connected between inner annular walls of the annular boss; an annular collecting tank with an opening at the top is arranged between the annular boss and the inner barrel wall of the outer liquid storage barrel 2, and collecting liquid is injected into the annular collecting tank; the inner liquid storage barrel is provided with double-layer liquid storage areas which are separated from each other so as to respectively inject precursor solutions with two different components; the outer side of the inner liquid storage barrel is provided with a micro-channel mechanism, one side of the micro-channel mechanism is provided with two connecting ends which are communicated with the two liquid storage areas in a one-to-one correspondence manner, the other side of the micro-channel mechanism is provided with a collecting end, and the collecting end is positioned above the collecting groove; preferably, the inner ring wall of the annular boss is provided with an inner thread section 2-1, the outer side of the bottom end of the inner liquid storage barrel is provided with an outer thread section 1-1, and the inner thread section 2-1 is screwed into the outer thread section 1-1 to fixedly connect the inner liquid storage barrel between the inner ring walls of the annular boss.
Still include a centrifugal drive mechanism, centrifugal drive mechanism is connected with outer liquid storage bucket 2, it can drive outer liquid storage bucket and interior liquid storage bucket and rotate at high speed in step and further make the precursor solution in two-layer liquid storage district be centrifugal motion in interior liquid storage bucket, the centrifugal force that two kinds of precursor solutions received when doing centrifugal motion can extrude the two and enter into the microchannel mechanism through the link that corresponds respectively in, and collect the end at microchannel mechanism and collect and form the liquid droplet, the liquid droplet of formation can fall into in the collecting liquid in the collecting vat under inertial action.
Specifically, the centrifugal transmission mechanism comprises a motor connecting sleeve 3, an upper connecting disc 3-2 is arranged at the top of the motor connecting sleeve 3, the bottom end of the outer liquid storage barrel 2 is fixedly connected with the upper connecting disc 3-2, a longitudinal through hole 3-1 is arranged at the bottom of the motor connecting sleeve 3, and a motor shaft of the speed regulating motor is fixedly arranged in the longitudinal through hole 3-1, so that the speed regulating motor can drive the outer liquid storage barrel 2 and the inner liquid storage barrel to synchronously rotate at high speed through the motor connecting sleeve 3.
Wherein, the upper connecting disc 3-2 is provided with a nut slot 3-3, and a nut is pre-installed in the nut slot 3-3; the bottom of the outer liquid storage barrel 2 is provided with a screw groove hole 2-2, and a screw with a proper type is selected to pass through the screw groove hole 2-2 and be screwed into a corresponding nut in the nut groove hole 3-3, so that the upper connecting disc 3-2 and the outer liquid storage barrel 2 can be connected and fixed.
As shown in fig. 1, the inner liquid storage barrel is a double-layer liquid storage barrel 1, a horizontal partition plate is arranged in the inner cavity of the double-layer liquid storage barrel 1, and a lower-layer liquid storage area 1-3 is defined between the bottom of the partition plate and the inner wall of the double-layer liquid storage barrel 1; the top of double-deck liquid storage bucket 1 is equipped with and extends and run through the transfusion hole to the baffle bottom towards its staving inner chamber, and the top edge of transfusion hole is equipped with ascending tubulose boss, and the top of tubulose boss highly is less than the inner chamber top of double-deck liquid storage bucket 1, encloses into upper reservoir district 1-2 between the outer wall of the top of baffle and tubulose boss and the interior bucket wall of double-deck liquid storage bucket 1.
The micro-channel mechanism comprises an upper capillary 1-4 and a lower capillary 1-5 which are arranged in parallel, a bifurcation end and a gathering end are arranged between the upper capillary 1-4 and the lower capillary 1-5, the orifice of the upper capillary 1-4 and the orifice of the upper capillary 1-4 at the bifurcation end are respectively and correspondingly connected to the upper layer liquid storage area 1-2 and the lower layer liquid storage area 1-3, the gathering capillary 1-6 is arranged at the outer side of the gathering end, and precursor solution flowing out of the upper capillary 1-4 and the lower capillary 1-5 can be gathered in the gathering capillary 1-6 to flow out to form liquid drops.
Second embodiment:
different from the first embodiment, as shown in fig. 2, the inner liquid storage barrel in this embodiment is no longer a double-layered liquid storage barrel 1 layered up and down; in the embodiment, a nested liquid storage barrel 4 is coaxially arranged in the inner cavity of the inner liquid storage barrel, and the inner cavity of the nested liquid storage barrel 4 is an inner-layer liquid storage area 4-2; the bottom end of the nested liquid storage barrel 4 is fixed on the bottom surface of the inner cavity of the inner liquid storage barrel in a sealing mode, an outer liquid storage area 4-1 is defined between the outer wall of the nested liquid storage barrel 4 and the inner wall of the inner liquid storage barrel, and a liquid inlet hole penetrating into the inner cavity of the nested liquid storage barrel 4 is formed in the top of the inner liquid storage barrel and used for injecting corresponding precursor solution.
Correspondingly, the micro-channel mechanism in the embodiment is also changed in structure, the micro-channel mechanism in the embodiment comprises an external phase capillary tube 4-3 and an internal phase capillary tube 4-4, one end of the external phase capillary tube 4-3 is communicated with the external layer liquid storage area 4-1, and the other end of the external phase capillary tube 4-3 extends out of the internal liquid storage barrel; one end of the inner phase capillary 4-4 is communicated with the inner layer liquid storage area 4-2, the other end of the inner phase capillary 4-4 is coaxially arranged in the outer phase capillary 4-3, and precursor solution in the inner phase capillary 4-4 and the outer phase capillary 4-3 can be collected and flow out at a port of the outer phase capillary 4-3 extending out of the inner liquid storage barrel to form liquid drops.
Wherein, microchannel mechanism sets up to the multiunit, and multiunit microchannel mechanism is equallyd divide equally to be installed in the outside of interior liquid storage bucket such as angle.
Wherein, the inner diameter of the external phase capillary tube 4-3 is 0.3-3mm, the outer diameter is 2-6mm, and the height is 1-5 mm.
The parts of this embodiment that are not described above are the same as those of the first embodiment, and are not described herein again.
The third embodiment:
the embodiment provides a method for preparing liquid drops based on the microfluidic device, which specifically comprises the following steps:
step a: preparing precursor solutions and collecting solutions with different components, and selecting a capillary tube with a proper pipe diameter and an inner liquid storage barrel with a proper diameter according to the size requirement of droplet preparation to complete the assembly of the microfluidic device;
step b: respectively injecting precursor solutions of the two components into the two-layer liquid storage areas, and injecting a collecting liquid into a collecting tank between the inner walls of the outer liquid storage barrel;
step c: setting the rotating speed of a speed regulating motor, driving an inner liquid storage barrel to rotate at a high speed by using the speed regulating motor, and further enabling precursor solutions with two different components to respectively do centrifugal motion in corresponding liquid storage areas so as to extrude the two precursor solutions into the capillary from one end of the corresponding capillary and generate liquid drops at the collecting end of the two capillaries;
step d: collecting the liquid drops by using the collecting liquid in the collecting tank, and carrying out post-treatment on the liquid drops in the collecting liquid to obtain the required gel microsphere product.
In the step a, the inner diameter of the capillary is 0.05-3mm, and the capillary is made of one of a glass capillary, a stainless steel capillary and a silica gel capillary; in step c, the speed setting range of the speed regulating motor is 400-5000 rpm.
In the step a, the prepared precursor solution is one of a sodium alginate solution, a chitosan solution and an oil phase liquid, and the oil phase liquid is one of peanut oil, sunflower seed oil, olive oil, soybean oil, corn oil, silicone oil and fluorinated oil; when the precursor solution is a sodium alginate solution or a chitosan solution, one or more of ink, fluorescent dye and fluorescent polystyrene nano-microspheres can be added into the prepared precursor solution.
Wherein the solvent of the chitosan solution is glacial acetic acid solution with volume fraction of 0.5-3%.
To better illustrate the specific process of droplet preparation using the above described apparatus, the present invention provides the following examples:
the fourth embodiment:
a method for rapidly preparing Janus gel microspheres by using the centrifugal microfluidic device in the first embodiment comprises the following specific implementation steps:
(1) formulating hydrogel precursor solutions and collections
Hydrogel precursor solution: mixing carbon ink and pure water 1: 199 as a solvent, preparing a sodium alginate solution with the mass fraction of 1.5%, magnetically stirring at 60 ℃ for 2 hours to obtain a hydrogel precursor solution a, mixing the fluorescent polystyrene nano-microspheres with pure water 1: 199 to be used as a solvent, preparing a sodium alginate solution with the mass fraction of 2%, and magnetically stirring the solution at the temperature of 60 ℃ for 4 hours to be used as a hydrogel precursor solution b;
collecting liquid: preparing a calcium chloride solution with the mass fraction of 2% as a collecting solution.
(2) Construction of centrifugal microfluidic devices
Firstly, fixing the starting ends of upper capillaries 1-4 and lower capillaries 1-5 in small holes on the side walls of an upper liquid storage area 1-2 and a lower liquid storage area 1-3 respectively, sleeving converging capillaries 1-6 on the converging ends of the upper capillaries 1-4 and the lower capillaries 1-5 at the same time, putting stainless steel screw caps into screw cap slotted holes 3-3, and using a small amount of AB glue for reinforcement; inserting stainless steel screws into the screw groove holes 2-2 and screwing down, so that the motor connecting sleeve 3 is connected with the outer liquid storage barrel 2; the motor connecting sleeve 3 is inserted into a motor rotating shaft, and the double-layer liquid storage barrel 1 and the outer liquid storage barrel 2 are screwed up through matched thread sections, as shown in figure 1.
(3) Production of Janus gel microspheres
Injecting a hydrogel precursor solution a into an upper layer liquid storage area 1-2 by using an injector, injecting a hydrogel precursor solution b into a lower layer liquid storage area 1-3, injecting a collecting liquid into a collecting tank, adjusting a motor driver so as to set the centrifugal speed to be 2000rpm, pressing a start button, rotating a double-layer liquid storage barrel 1 at a high speed, extruding the hydrogel precursor solutions a and b through a micro-channel formed by capillaries under the action of centrifugal force, forming sodium alginate droplets at the tail ends of the collecting capillaries 1-6, making parabolic motion, then dropping into a calcium chloride solution in the collecting tank, and carrying out ion crosslinking reaction to generate Janus gel microspheres.
Fifth embodiment:
a method for rapidly preparing sodium alginate-oil core-shell microspheres by using the centrifugal microfluidic device in the second embodiment comprises the following specific implementation steps:
(1) formulating hydrogel precursor solution, oil phase and harvest
Hydrogel precursor solution: preparing a sodium alginate solution with the mass fraction of 2%, and magnetically stirring for 2 hours at the temperature of 60 ℃ to obtain a hydrogel precursor solution;
oil phase: mixing 50CS silicone oil and 500CS silicone oil in a ratio of 3: 2 mixing uniformly to obtain an oil phase.
Collecting liquid: preparing a calcium chloride solution with the mass fraction of 3% as a collecting solution.
(2) Construction of centrifugal microfluidic devices
Firstly, fixing the initial ends of an inner capillary tube 4-4 and an outer capillary tube 4-3 in the communicating holes on the side walls of an inner layer liquid storage area 4-2 and an outer layer liquid storage area 4-1 respectively, wherein the tail ends of the inner capillary tube 4-4 and the outer capillary tube 4-3 are collecting ends, putting a stainless steel screw cap into a screw cap slotted hole 3-3, and using a small amount of AB glue for reinforcement; inserting stainless steel screws into the screw groove holes 2-2 and screwing down, so that the motor connecting sleeve 3 is connected with the outer liquid storage barrel 2; the motor connecting sleeve 3 is inserted into the motor rotating shaft, and the nested liquid storage barrel 4 and the outer liquid storage barrel 2 are screwed up through threads, as shown in figure 2.
(3) Generation of sodium alginate-oil core-shell microspheres
Injecting an oil phase into the inner-layer liquid storage area 4-2 by using an injector, injecting a hydrogel precursor solution into the outer-layer liquid storage area 4-1, injecting a collecting liquid into a collecting tank, adjusting a motor driver to set the centrifugal speed to be 1800rpm, pressing a start button, synchronously rotating the inner liquid storage barrel and the nested liquid storage barrel 4 at a high speed, extruding the oil phase and the hydrogel precursor solution through a micro-channel formed by capillaries under the action of centrifugal force to form oil-in-water droplets, dropping into a calcium chloride solution in the collecting tank after parabolic motion, and performing ion crosslinking reaction on the outer-layer sodium alginate and the calcium chloride to generate the sodium alginate-oil core-shell microspheres.
Sixth embodiment:
a method for rapidly preparing chitosan-oil core-shell microspheres by using the centrifugal microfluidic device in the second embodiment comprises the following specific implementation steps:
(1) formulating hydrogel precursor solution, oil phase and harvest
Hydrogel precursor solution: preparing a chitosan solution with the mass fraction of 4% by taking a glacial acetic acid aqueous solution with the volume fraction of 2% as a solvent, and magnetically stirring for 4 hours at room temperature to obtain a hydrogel precursor solution;
oil phase: 100CS silicone oil was used as the oil phase.
Collecting liquid: preparing a sodium tripolyphosphate solution with the mass fraction of 2% as a collecting solution.
(2) Construction of centrifugal microfluidic devices
Firstly, fixing the initial ends of an inner capillary tube 4-4 and an outer capillary tube 4-3 in the communicating holes on the side walls of an inner layer liquid storage area 4-2 and an outer layer liquid storage area 4-1 respectively, wherein the tail ends of the inner capillary tube 4-4 and the outer capillary tube 4-3 are collecting ends, putting a stainless steel screw cap into a screw cap slotted hole 3-3, and using a small amount of AB glue for reinforcement; inserting stainless steel screws into the screw groove holes 2-2 and screwing down, so that the motor connecting sleeve 3 is connected with the outer liquid storage barrel 2; the motor connecting sleeve 3 is inserted into the motor rotating shaft, and the nested liquid storage barrel 4 and the outer liquid storage barrel 2 are screwed up through threads, as shown in figure 2.
(3) Generation of chitosan-oil core-shell microspheres
Injecting an oil phase into the inner layer liquid storage area 4-2 by using an injector, injecting a hydrogel precursor solution into the outer layer liquid storage area 4-1, injecting a collecting liquid into a collecting tank, adjusting a motor driver to set the centrifugal speed to be 1600rpm, pressing a start button, synchronously rotating the inner liquid storage barrel and the nested liquid storage barrel 4 at a high speed, extruding the oil phase and the hydrogel precursor solution through a micro-channel formed by capillaries under the action of centrifugal force to form oil-in-water droplets, dropping into a sodium tripolyphosphate solution in the collecting tank after parabolic motion, and performing chemical crosslinking reaction on the outer layer chitosan and the sodium tripolyphosphate to generate the chitosan-oil core-shell microspheres.
Above only the utility model discloses an it is preferred embodiment, the utility model discloses a scope of protection not only limits in above-mentioned embodiment, and the all belongs to the utility model discloses a technical scheme under the thinking all belongs to the utility model discloses a scope of protection. It should be noted that, for those skilled in the art, a plurality of modifications and decorations without departing from the principle of the present invention should be considered as the protection scope of the present invention.
Claims (7)
1. A centrifugal microfluidic device for rapidly preparing double-component gel microspheres comprises an inner liquid storage barrel and an outer liquid storage barrel, and is characterized in that the inner liquid storage barrel is coaxially arranged in an inner cavity of the outer liquid storage barrel, an annular boss extending to the top of the inner cavity of the outer liquid storage barrel is arranged at the bottom end of the inner cavity of the outer liquid storage barrel, and the bottom end of the inner liquid storage barrel is fixedly connected between inner ring walls of the annular boss; a collecting tank with an opening at the top is arranged between the annular boss and the inner barrel wall of the outer liquid storage barrel, and collecting liquid is injected into the collecting tank; the inner liquid storage barrel is provided with double-layer liquid storage areas which are separated from each other so as to respectively inject precursor solutions with two different components; the outer side of the inner liquid storage barrel is provided with a micro-channel mechanism, one side of the micro-channel mechanism is provided with two connecting ends which are communicated with the two liquid storage areas in a one-to-one correspondence manner, the other side of the micro-channel mechanism is provided with a collecting end, and the collecting end is positioned above the collecting groove;
still include a centrifugal drive mechanism, centrifugal drive mechanism is connected with outer liquid storage bucket, and it can drive outer liquid storage bucket and interior liquid storage bucket and rotate at high speed in step and further make the precursor solution in two-layer liquid storage district be centrifugal motion in interior liquid storage bucket, two the centrifugal force that precursor solution received when doing centrifugal motion can extrude the two and enter into the microchannel mechanism through the link that corresponds respectively in to end collection at microchannel mechanism collects and forms the liquid droplet, and the liquid droplet of formation can fall into in the collecting fluid in the collecting vat under the inertial action.
2. The rapid preparation centrifugal microfluidic device of two-component gel microspheres of claim 1, wherein: the centrifugal transmission mechanism comprises a motor connecting sleeve, an upper connecting disc is arranged at the top of the motor connecting sleeve, the bottom end of the outer liquid storage barrel is fixedly connected with the upper connecting disc, a longitudinal through hole is formed in the bottom of the motor connecting sleeve, and a motor shaft of the speed regulating motor is fixedly arranged in the longitudinal through hole, so that the speed regulating motor can drive the outer liquid storage barrel and the inner liquid storage barrel to synchronously rotate at high speed through the motor connecting sleeve.
3. The rapid preparation centrifugal microfluidic device of two-component gel microspheres of claim 2, wherein: a horizontal partition plate is further arranged in the inner cavity of the barrel body of the inner liquid storage barrel, and a lower-layer liquid storage area is defined between the bottom of the partition plate and the inner wall of the barrel body of the inner liquid storage barrel; the top of interior liquid storage bucket is equipped with and extends and run through the transfusion hole to the baffle bottom towards its staving inner chamber, the top edge of transfusion hole is equipped with ascending tubulose boss, the top height of tubulose boss is less than the inner chamber top of interior liquid storage bucket, enclose into upper reservoir district between the top of baffle and the outer wall of tubulose boss and the interior bucket wall of interior liquid storage bucket.
4. The rapid preparation centrifugal microfluidic device of two-component gel microspheres of claim 2, wherein: an inner cavity of the inner liquid storage barrel is coaxially provided with an embedded liquid storage barrel, and the inner cavity of the embedded liquid storage barrel is an inner-layer liquid storage area; the bottom end of the nested liquid storage barrel is fixed on the bottom surface of the inner cavity of the inner liquid storage barrel in a sealing mode, an outer liquid storage area is defined between the outer wall of the nested liquid storage barrel and the inner wall of the inner liquid storage barrel, and a liquid inlet hole penetrating into the inner cavity of the nested liquid storage barrel is formed in the top of the inner liquid storage barrel.
5. The rapid preparation centrifugal microfluidic device of the bi-component gel microspheres of claim 3, wherein: the micro-channel mechanism comprises an upper capillary and a lower capillary which are arranged in parallel, a bifurcation end and a gathering end are arranged between the upper capillary and the lower capillary, an upper capillary pipe orifice and a lower capillary pipe orifice at the bifurcation end are respectively and correspondingly connected to an upper layer liquid storage area and a lower layer liquid storage area, a gathering capillary is arranged on the outer side of the gathering end, and precursor solution flowing out of the upper capillary and the lower capillary can flow out in the gathering capillary to form liquid drops.
6. The rapid preparation centrifugal microfluidic device of the bi-component gel microspheres of claim 4, wherein: the micro-channel mechanism comprises an inner phase capillary tube and an outer phase capillary tube, one end of the outer phase capillary tube is communicated with the outer layer liquid storage area, and the other end of the outer phase capillary tube extends out of the inner liquid storage barrel; one end of the inner phase capillary tube is communicated with the inner liquid storage area, the other end of the inner phase capillary tube is coaxially arranged in the outer phase capillary tube, and precursor solution flowing out of the inner phase capillary tube and the outer phase capillary tube can be collected and flow out at a port of the outer phase capillary tube, which extends out of the inner liquid storage barrel, so as to form liquid drops.
7. The rapid preparation centrifugal microfluidic device of two-component gel microspheres of claim 5 or 6, wherein: the microchannel mechanisms are arranged into a plurality of groups, and the plurality of groups of microchannel mechanisms are equally and angularly arranged on the outer side of the inner liquid storage barrel.
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN114231397A (en) * | 2021-12-21 | 2022-03-25 | 中国医科大学 | High-throughput drug testing centrifugal microfluidic system and use method thereof |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114231397A (en) * | 2021-12-21 | 2022-03-25 | 中国医科大学 | High-throughput drug testing centrifugal microfluidic system and use method thereof |
| CN114231397B (en) * | 2021-12-21 | 2024-02-23 | 中国医科大学 | Centrifugal microfluidic system for high-flux drug testing and application method thereof |
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