CN214881576U

CN214881576U - Automatic liquid feeding and supplementing device for cell culture

Info

Publication number: CN214881576U
Application number: CN202023344859.4U
Authority: CN
Inventors: 初相伍; 曹启龙; 梁少帅; 李环宇; 王飞; 胡艳华
Original assignee: Qingdao Haier Biotechnology Co ltd
Current assignee: Qingdao Haier Biotechnology Co ltd
Priority date: 2020-12-31
Filing date: 2020-12-31
Publication date: 2021-11-26
Anticipated expiration: 2030-12-31

Abstract

The utility model relates to the technical field of biological culture devices, in particular to an automatic liquid feeding and supplementing device for cell culture, which comprises a workbench arranged in a biological safety cabinet, a driving device arranged at the bottom of the workbench, and a rotating disc arranged above the workbench, wherein the driving device drives the rotating disc to rotate, a plurality of supporting devices are arranged on the rotating disc, each supporting device comprises a supporting bottom plate, a supporting inclined plate and a supporting upright post, an injection device is arranged above the supporting upright post, the injection device drives a piston rod of an injector body to move up and down, the head of the injector body is connected with an injection joint, the injection joint is connected with an opening of a culture bag through a pipeline, the production line type liquid feeding and supplementing process can be realized in a smaller space, the culture bag placed on the supporting inclined plate in batches is automatically controlled by the driving device to rotate to a station, the liquid feeding or supplementing amount is automatically and quantitatively controlled by the injection device, reduce the walking of people and reduce the cell pollution.

Description

Automatic liquid feeding and supplementing device for cell culture

Technical Field

The utility model relates to a biological culture device technical field specifically is an automatic liquid feeding fluid infusion device of cell culture.

Background

Various operations such as bottle rotating, liquid adding, bagging and feedback are needed to be carried out in the cell culture process, various processes can only be operated by operators, the liquid adding and bagging operations are needed to be carried out on a large batch of cell culture bags due to the large workload, and the bags are needed to be rotated after the bagging is completed, so that the cell culture bags are needed to be assisted by tools.

At present, the conventional operation is to connect a culture bag through an injection tube of a 50ml injector, connect the culture bag through the injection tube of the injector, then place the culture bag on a fluid infusion frame, and then perform fluid infusion operation like using the injector as a funnel. When the amount of cell fluid infusion is added by pouring from the opening to the inside, an operation instrument for assisting cell fluid infusion in the market, such as an electric pipette, can be directly adopted; however, when the amount of cell fluid infusion is large, the technical conditions limit the operation, and technical operators often adopt direct dumping or use an electric pipette to perform repeated operation for many times, which both increase technical operation difficulty and cell contamination probability to different degrees.

In order to lighten the physical burden of workers and liberate both hands, the liquid supplementing amount is controlled conveniently, the Chinese utility model patent with the patent number of ZL2018204834311 discloses that "stainless steel frame is collected in the liquid supplementing of cell", the Chinese utility model patent with the patent number of ZL2017203396673 discloses that "a liquid supplementing collection device suitable for with cell bag" all provides a solid type liquid supplementing support, can fix the liquid supplementing pipe on the support and carry out the liquid supplementing. However, the existing fluid infusion support cannot fix the cell culture bag, and because large-capacity containers such as the cell culture bag are soft plastic bags, the bag body cannot stand upright, the large-capacity containers are generally directly and horizontally placed on an operation table, and the large-capacity containers are inconvenient to connect and install.

In the prior art, culture bags and culture media need to be taken and placed back and forth and the culture media need to be poured or moved into an injection tube for multiple times in the liquid adding and supplementing processes, so that the probability of cell pollution is increased, the space is small in a biological safety cabinet, the flow of personnel is reduced, the operation flow is reduced, the cell pollution is avoided, and the amount of fluid supplementation cannot be controlled by the existing injector.

Disclosure of Invention

The utility model aims at the above-mentioned problem that has cell culture liquid feeding and fluid infusion flow loaded down with trivial details, needs operating personnel more and current fluid infusion can not control the fluid infusion volume that exists, provides an automatic liquid feeding fluid infusion device of cell culture.

In order to solve the above problem, the utility model provides a following technical scheme: an automatic liquid feeding and supplementing device for cell culture comprises a workbench arranged in a biological safety cabinet, a driving device arranged at the bottom of the workbench, and a rotating disc arranged above the workbench, wherein the driving device drives the rotating disc to rotate, the rotating disc is provided with a plurality of supporting devices, each supporting device comprises a supporting bottom plate, a supporting inclined plate and a supporting upright post, the supporting upright posts are arranged in the middle of the rotating disc, the supporting bottom plates are distributed on the rotating disc in a circumferential array manner, one end of each supporting inclined plate is hinged with the end part of each supporting bottom plate, the other end of each supporting inclined plate is arranged on each supporting upright post through a hook, an injection device is arranged above each supporting upright post, the injection device drives a piston rod of an injector body to move up and down, and the head of the injector body is connected with an injection joint, the injection connector is connected with the opening of the culture bag through a pipeline.

Further, drive arrangement include driving motor, reduction gear and transmission, transmission include transmission case and fixed disk, the fixed disk embedding on the workstation, the transmission case is installed to the fixed disk below, the transmission case in be equipped with bearing and gear wheel, driving motor pass through reduction gear and little gear connection, pinion and gear wheel meshing, the gear wheel passes through the rotation of axle drive rolling disc.

Furthermore, the workbench is provided with an injection bracket which spans two sides of the workbench, the injection device comprises an injection bottom plate, a vertical cylinder, a vertical slide block, an injection cylinder, an injection slide block, a push plate, a clamping block, a clamping plate and a clamping cylinder, the injection bottom plate is arranged on the injection bracket, a vertical cylinder is arranged above the injection bottom plate, the vertical cylinder drives the vertical sliding block to move up and down, the top of the vertical sliding block is provided with an injection cylinder, the injection cylinder drives the injection slide block to move up and down, the injection slide block is provided with a push plate, the push plate applies pressure to the end part of the piston rod of the injector body to drive the piston rod of the injector body to move up and down, the injector body is arranged on two clamping plate supports, the clamping block is positioned at the end part of the clamping plate and wraps the injector body, and one of the two clamping plates is driven by the clamping cylinder and clamps the injector body.

Furthermore, the clamping blocks are fixed on the vertical sliding blocks through manual clamps.

Further, the clamping cylinder is arranged on the side part of the vertical sliding block through a bracket.

Furthermore, the supporting bottom plate is a trapezoidal plate.

Furthermore, the upper surface of the supporting inclined plate is provided with an anti-skid layer.

Furthermore, the manual clamp is a bolt type quick clamp.

Furthermore, the workbench is provided with a sliding groove, the bottom of the supporting plate is provided with a sliding block, and the sliding block can move along the inner circumference of the sliding groove.

Compared with the prior art, the beneficial effects of the utility model are that:

1. the automatic cell culture solution adding and supplementing device is arranged in the biological safety cabinet, so that a production line type liquid adding and supplementing process can be realized in a smaller space, culture bags placed on the supporting inclined plate in batches are automatically controlled by the driving device to rotate to a station, and the liquid adding or supplementing amount is automatically and quantitatively controlled by the injection device, so that the walking of personnel is reduced, and the cell pollution is reduced;

2. the culture bags are placed on the supporting inclined plate in batches, the rotating disc is controlled to rotate through the driving motor, so that the culture bags needing to be operated are rotated to the station, the material preparation before liquid adding and liquid supplementing is completed quickly, and the working efficiency is improved.

3. Accomplish the installation of syringe body fast through manual clamp, place the syringe body in suitable position, through the fixed syringe body of die clamping cylinder, make the head and the injection articulate of syringe body through vertical cylinder, the volume of syringe body can supply a plurality of culture bag liquid feeding or fluid infusion, accomplishes the injection through injection cylinder, and degree of automation is high to the steerable liquid feeding of injection cylinder or fluid infusion volume.

4. The culture bag is placed on the inclined supporting plate in an inclined mode, liquid adding or liquid supplementing is facilitated, and the anti-slip layer prevents the culture bag from slipping.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

Fig. 1 is a schematic perspective view of the present invention;

fig. 2 is a front view of the present invention;

fig. 3 is a schematic view of the driving structure of the present invention;

fig. 4 is a bottom view of the present invention;

fig. 5 is a top view of the present invention;

fig. 6 is a first schematic structural diagram of the injection device of the present invention;

fig. 7 is a schematic structural diagram of an injection device according to the present invention.

In the figure: 1. a work table; 2. a drive motor; 3. a speed reducer; 4. a transmission device; 5. a chute; 6. rotating the disc; 7. a support device; 8. an injection stent; 9. an injection device; 10. an injector body; 11. an injection fitting; 31. a pinion gear; 41. a transmission case; 42. a bull gear; 43. a bearing; 44. fixing the disc; 71. a support base plate; 72. supporting the inclined plate; 73. supporting the upright post; 74. a slider; 101. an injection base plate; 102. a vertical cylinder; 103. a vertical slide block; 104. an injection cylinder; 105. an injection slide block; 106. pushing the plate; 107. a clamping block; 108. a clamping cylinder; 109. a splint; 110. and (5) manually clamping.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

In the description of the present invention, it should be noted that the terms "upper", "lower", "inner", "outer", "front end", "rear end", "both ends", "one end", "the other end" and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element to which the reference is made must have a specific orientation, be constructed in a specific orientation, and be operated, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.

In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "disposed," "connected," and the like are to be construed broadly, and for example, "connected" may be either fixedly connected or detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

As shown in figures 1-7

Example one

The utility model provides a following technical scheme: an automatic liquid feeding and supplementing device for cell culture comprises a workbench 1 arranged in a biological safety cabinet, a driving device arranged at the bottom of the workbench 1 and a rotating disc 6 arranged above the workbench 1, wherein the driving device drives the rotating disc 6 to rotate, a plurality of supporting devices 7 are arranged on the rotating disc 6, each supporting device 7 comprises a supporting bottom plate 71, a supporting inclined plate 72 and a supporting upright post 73, the supporting upright posts 73 are arranged in the middle of the rotating disc 6, the supporting bottom plates 71 are distributed on the rotating disc 6 in a circumferential array manner, one end of each supporting inclined plate 72 is hinged with the end part of each supporting bottom plate 71, the other end of each supporting inclined plate 72 is arranged on the corresponding supporting upright post 73 through a hook, an injection device 9 is arranged above the corresponding supporting upright post 73, and the injection device 9 drives a piston rod of an injector body 10 to move up and down, the head of the injector body 10 is connected with an injection joint 11, and the injection joint 11 is connected with the opening of the culture bag through a pipeline.

Drive arrangement includes driving motor 2, reduction gear 3 and transmission 4, transmission 4 include transmission case 41 and fixed disk 44, fixed disk 44 embedding on workstation 1, transmission case 41 is installed to fixed disk 44 below, transmission case 41 in be equipped with bearing 43 and gear wheel 42, driving motor 2 be connected with pinion 31 through reduction gear 3, pinion 31 and gear wheel 42 mesh, gear wheel 42 rotates through axle drive rolling disc 6.

The injection device 9 comprises an injection bottom plate 101, a vertical cylinder 102, a vertical slide block 103, an injection cylinder 104, an injection slide block 105, a push plate 106, a clamping block 107, a clamping plate 109 and a clamping cylinder 108, wherein the injection bottom plate 101 is installed on the injection support 8, the vertical cylinder 102 is arranged above the injection bottom plate 101, the vertical cylinder 102 drives the vertical slide block 103 to move up and down, the injection cylinder 104 is arranged at the top of the vertical slide block 103, the injection cylinder 104 drives the injection slide block 105 to move up and down, the push plate 106 is arranged on the injection slide block 105, the push plate 106 applies pressure to the end part of a piston rod of the injector body 10 to drive the piston rod of the injector body 10 to move up and down, the injector body 10 is installed on the two clamping plate 109 supports, and the clamping block 107 is positioned at the end part of the clamping plate 109 to coat the injector body 10, one of the two clamping plates 109 is driven by the clamping cylinder 108 and clamps the injector body 10.

The clamp block 107 is fixed to the vertical slide 103 by a manual clamp 110. The manual clamp 110 is a bolt-on quick clamp.

The clamping cylinder 108 is mounted on the side of the vertical slider 103 by a bracket.

The support base 71 is a trapezoidal plate. The upper surface of the supporting sloping plate 72 is provided with an anti-slip layer.

The worktable 1 is provided with a sliding chute 5, the bottom of the supporting bottom plate 71 is provided with a sliding block 74, and the sliding block 74 can move along the inner circumference of the sliding chute 5.

When the utility model is used, firstly, the utility model is placed in a biological safety cabinet, the position of the injector body 10 which is easy to be installed is taken as the position facing to an operator, when the operation of liquid adding or liquid supplementing is carried out, whether the supporting sloping plate 72 is lifted up is determined according to the supporting body adopted by the culture device, if the supporting body of the culture medium is a culture bottle, the supporting body is not lifted up, if the supporting body of the culture medium is a culture bag, the supporting body is lifted up and fixed on the supporting upright post 73 through a hook, the utility model is suitable for the supporting bodies of various culture mediums, then the culture bottles or the culture bags are placed on the supporting sloping plate 72 in batch, the culture medium is sucked into the injector body 10, the volume of the injector body 10 can be used for adding liquid or supplementing liquid for a plurality of culture bottles or culture bags, scales are arranged on the injector body 10, then the injector body 10 is installed on the injection device 9, then the operator is right ahead of a station, the operation of carrying out first culture bag or blake bottle, through opening the switch of injection cylinder 104, carry out the liquid feeding, inside the interior culture medium of syringe body 10 inserts blake bottle or culture medium behind injection joint 11, the stroke of injection cylinder 104 represents the injection volume, calculate the liquid feeding through controller and computer, then control the opening time of injection cylinder 104, after the injection finishes, open driving motor 2, make the backup pad of next position just to operating personnel, carry out the liquid feeding of another time, accomplish the liquid feeding operation back of all positions, shift again, consequently, personnel's ambulation has been reduced, the risk of cell contamination has been reduced.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Claims

1. The utility model provides an automatic liquid feeding fluid infusion device of cell culture which characterized in that: comprises a workbench (1) arranged in a biological safety cabinet, a driving device arranged at the bottom of the workbench (1) and a rotating disc (6) arranged above the workbench (1), wherein the driving device drives the rotating disc (6) to rotate, a plurality of supporting devices (7) are arranged on the rotating disc (6), each supporting device (7) comprises a supporting bottom plate (71), a supporting inclined plate (72) and a supporting upright post (73), the supporting upright posts (73) are arranged in the middle of the rotating disc (6), the supporting bottom plates (71) are distributed on the rotating disc (6) in a circumferential array manner, one end of each supporting inclined plate (72) is hinged with the end part of each supporting bottom plate (71), the other end of each supporting inclined plate (72) is arranged on each supporting upright post (73) through a hook, and an injection device (9) is arranged above each supporting upright post (73), the injection device (9) drives a piston rod of the injector body (10) to move up and down, the head of the injector body (10) is connected with the injection joint (11), and the injection joint (11) is connected with an opening of the culture bag through a pipeline.

2. The automatic liquid adding and supplementing device for cell culture according to claim 1, characterized in that: drive arrangement includes driving motor (2), reduction gear (3) and transmission (4), transmission (4) include transmission case (41) and fixed disk (44), fixed disk (44) embedding on workstation (1), transmission case (41) are installed to fixed disk (44) below, transmission case (41) in be equipped with bearing (43) and gear wheel (42), driving motor (2) be connected with pinion (31) through reduction gear (3), pinion (31) and gear wheel (42) meshing, gear wheel (42) rotate through axle drive rolling disc (6).

3. The automatic liquid adding and supplementing device for cell culture according to claim 2, characterized in that: an injection support (8) stretching across two sides of the workbench (1) is arranged on the workbench (1), the injection device (9) comprises an injection bottom plate (101), a vertical cylinder (102), a vertical sliding block (103), an injection cylinder (104), an injection sliding block (105), a push plate (106), a clamping block (107), a clamping plate (109) and a clamping cylinder (108), the injection bottom plate (101) is arranged on the injection support (8), the vertical cylinder (102) is arranged above the injection bottom plate (101), the vertical cylinder (102) drives the vertical sliding block (103) to move up and down, the injection cylinder (104) is arranged at the top of the vertical sliding block (103), the injection cylinder (104) drives the injection sliding block (105) to move up and down, the push plate (106) is arranged on the injection sliding block (105), and the push plate (106) applies pressure to the end part of the piston rod of the injector body (10) to drive the piston rod of the injector body (10) to move up and down, the injector body (10) is arranged on two clamping plate (109) supports, the clamping block (107) is positioned at the end part of the clamping plate (109) and covers the injector body (10), and one clamping plate (109) of the two clamping plates (109) is driven by a clamping cylinder (108) and clamps the injector body (10).

4. The automatic liquid adding and supplementing device for cell culture according to claim 3, wherein: the clamping block (107) is fixed on the vertical sliding block (103) through a manual clamp (110).

5. The automatic liquid adding and supplementing device for cell culture according to claim 4, wherein: the manual clamp (110) is a bolt type quick clamp.

6. The automatic liquid adding and supplementing device for cell culture according to claim 5, wherein: the clamping cylinder (108) is arranged on the side part of the vertical sliding block (103) through a bracket.

7. The automatic liquid adding and replenishing device for cell culture according to any one of claims 1 to 6, wherein: the supporting bottom plate (71) is a trapezoidal plate.

8. The automatic liquid adding and supplementing device for cell culture according to claim 1, characterized in that: the upper surface of the supporting sloping plate (72) is provided with an anti-slip layer.

9. The automatic liquid adding and replenishing device for cell culture according to claim 8, wherein: a sliding groove (5) is formed in the workbench (1), a sliding block (74) is arranged at the bottom of the supporting base plate (71), and the sliding block (74) can move along the inner circumference of the sliding groove (5).