CN214881407U - double-T-shaped generation channel integrated micro-fluidic chip and micro-fluidic chip assembly - Google Patents
double-T-shaped generation channel integrated micro-fluidic chip and micro-fluidic chip assembly Download PDFInfo
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- CN214881407U CN214881407U CN202120575667.XU CN202120575667U CN214881407U CN 214881407 U CN214881407 U CN 214881407U CN 202120575667 U CN202120575667 U CN 202120575667U CN 214881407 U CN214881407 U CN 214881407U
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- droplet
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- 239000000758 substrate Substances 0.000 claims abstract description 14
- 239000000126 substance Substances 0.000 claims abstract description 3
- 229920000642 polymer Polymers 0.000 claims abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000007788 liquid Substances 0.000 claims description 10
- 239000002699 waste material Substances 0.000 claims description 9
- 230000000903 blocking effect Effects 0.000 claims 1
- 238000001514 detection method Methods 0.000 abstract description 10
- 230000003321 amplification Effects 0.000 abstract description 7
- 238000003199 nucleic acid amplification method Methods 0.000 abstract description 7
- 238000007847 digital PCR Methods 0.000 abstract description 5
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 abstract 1
- 238000005516 engineering process Methods 0.000 description 6
- 238000003752 polymerase chain reaction Methods 0.000 description 3
- 230000009977 dual effect Effects 0.000 description 2
- 230000010354 integration Effects 0.000 description 2
- 239000002356 single layer Substances 0.000 description 2
- 108091028043 Nucleic acid sequence Proteins 0.000 description 1
- 238000012864 cross contamination Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
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Abstract
The utility model discloses a double-T-shaped generation channel integrated microfluidic chip and a microfluidic chip assembly, which comprises a channel layer and a substrate, wherein the channel layer and the substrate are made of high molecular polymers with good chemical resistance; the channel layer comprises a double T-shaped droplet generation structure and a droplet capture structure; the substrate is positioned below the channel layer, so that the structure of the channel layer is isolated from the outside; the method can enable the generation, the capture, the amplification and the detection of the microdroplets to be carried out on the same chip, and can be realized independently or integrally. The utility model discloses a two T type passageways generate the droplet, and contrast single channel generates the structure, has improved droplet generation efficiency, reduces sample preparation time. The utility model discloses a filter column type frame makes the droplet can catch on the coplanar, for the enrichment sample provides catches the space, also provides a more convenient platform for sample detection. The utility model discloses lower in structural cost, do benefit to production, be convenient for develop at digital PCR technical field and use.
Description
Technical Field
The utility model relates to a PCR technical field especially relates to two T types generate passageway integral type micro-fluidic chip, micro-fluidic chip subassembly.
Background
The micro-fluidic chip technology can integrate the basic functions of the traditional biological and chemical laboratories on a microchip, develops rapidly, and can generate a large number of micro-droplets which are not easy to cause cross contamination by utilizing a micro-structure, so that the micro-fluidic chip technology has great application potential in many biochemical fields.
Polymerase Chain Reaction (PCR) is an important technology that can realize in vitro amplification of DNA sequences to achieve nucleic acid detection, and third generation-digital PCR has been developed. In conjunction with digital PCR and microfluidic technologies, there exist digital PCR systems that can integrate droplet generation, amplification and detection. However, the three functions of droplet generation, amplification and detection are still independent of each other, and the integration and automation degree are not high.
The development of the image recognition technology provides a new idea for droplet detection, and if the droplets are amplified and then all on the same plane, the image recognition technology can be used for photographing, so that the detection efficiency is improved.
Aiming at the problems of the prior art and the digital PCR, a new microfluidic chip which has a simple structure, high integration level and high automation degree and can capture microdroplets on the same plane needs to be developed to realize cost control and rapid detection of samples.
SUMMERY OF THE UTILITY MODEL
The utility model aims at overcoming above-mentioned prior art's shortcoming and not enough, providing a simple structure practical two T types generate passageway integral type micro-fluidic chip, be applicable to the droplet and generate, the droplet is caught, experiments such as amplification.
In order to achieve the above purpose, the utility model adopts the following technical scheme:
a double-T-shaped generation channel integrated microfluidic chip comprises a chip substrate 1, wherein the substrate 1 is provided with an oil phase inlet 2, a water phase inlet 4, a T-shaped micro-droplet generation channel 3, a capture area 5 and a waste liquid outlet 6.
Further, the ratio of the channel depth of the chip substrate 1 to the droplet diameter is not more than 1.2: 1.
Further, the channel width of the T-shaped droplet generation channel 3 connected to the oil phase inlet 2 is not smaller than the channel width of the T-shaped droplet generation channel 3 connected to the water phase inlet 4.
Further, the channel connecting the T-shaped droplet generation channel 3 and the oil phase inlet 2 is perpendicular to the channel width connecting the T-shaped droplet generation channel 3 and the water phase inlet 4.
Further, the ratio of the distance from the end of the rectangular fence in the capture area 5 close to the T-shaped droplet generation channel 3 to the boundary of the capture area 5 to the droplet diameter is not less than 2.5: 1.
Further, the side of the fence in the capturing area 5 close to the capturing area 5 boundary is equal to the capturing area 5 boundary.
Further, the waste liquid outlet 6 is connected to the capture area 5 with a channel width not smaller than the droplet diameter.
The utility model has the advantages that:
1. the double-T-shaped channel integrated microfluidic chip is simple in structure and low in manufacturing cost, and can meet the requirements of most slide specifications.
2. The double-T-shaped channel integrated micro-fluidic chip has a double-droplet generation channel structure, and the generation efficiency is improved compared with a single-channel droplet generation channel.
3. The capture area of the double-T-shaped channel integrated microfluidic chip is a single-layer capture structure, so that a droplet single layer can be tiled in the capture area, the double-T-shaped channel integrated microfluidic chip is suitable for droplet amplification, and a platform is provided for subsequent image identification and detection.
Drawings
Fig. 1 is a schematic structural diagram of a dual T-shaped generation channel integrated microfluidic chip provided by the present invention;
fig. 2 is a central axis sectional view of the dual T-shaped integrated microfluidic chip.
In the figure, 1 a substrate, 2 an oil phase inlet, 3T type droplet generation channels, 4 a water phase inlet, 5 a capture area, 6 a waste liquid outlet, 7T type droplet generation channel oil phase inlet channels and 8T type droplet generation channel water phase inlet channels.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.
As shown in fig. 1. The utility model discloses a chip is caught to two T types generate micro-fluidic of passageway integral type, including the chip base member, change and contained two T type microdroplets in the chip base member and generated passageway 3, a capture region 5 that is used for catching the microdroplet.
Two T-shaped droplet generation channels 3 are axially symmetric about the chip, and the outlets are connected to capture regions 5. The generated oil and the sample are respectively introduced into the oil phase inlet 2 and the water phase inlet 4, and the size of the generated micro-droplets is adjusted by controlling the pressure of the two-phase inlets.
The rectangular fences in the capture zone 5 have a gap between the upper and lower fences on the side close to the waste liquid outlet much smaller than the diameter of the droplets, enabling the droplets to spread flat in the capture zone. The oil phase inlet 2 is controlled during amplification and detection, and the pressure of the water phase inlet 4 is the same as that of the waste liquid outlet 6.
The length of the chip substrate is 24mm, the width of the chip substrate is 17.5mm, and the thickness of the chip substrate is 4 mm.
The thickness of the T-shaped droplet generation channel 3 and the thickness of the capture area 5 are both 50 μm, the oil phase inlet channel 7 of the droplet generation channel 3 has a width of 120 μm, and the water phase inlet channel 8 has a width of 40 μm.
The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.
Claims (6)
1. The utility model provides a two T types generate passageway integral type micro-fluidic chip which characterized in that: comprises a channel layer and a substrate which are made of high molecular polymer with good chemical resistance; the channel layer comprises a double T-shaped droplet generation structure and a droplet capture structure; the substrate is positioned below the channel layer, so that the structure of the channel layer is isolated from the outside; wherein the droplet generation structure and the substrate form a microchannel for droplet generation; the droplet capture structure and the substrate form a microchannel for capturing droplets; the droplet generation microchannel is connected to the microchannel captured by the droplet.
2. The integrated microfluidic chip with double T-shaped generating channels as claimed in claim 1, wherein: the double T-shaped microdroplet generating structure has an oil phase inlet width Wo and a water phase inlet width Ww, wherein Ww is less than Wo.
3. The integrated microfluidic chip with double T-shaped generating channels according to claim 1, wherein: the droplet produces a microchannel having the same height as the microchannel of the droplet capture structure.
4. The integrated microfluidic chip with double T-shaped generating channels according to claim 1, wherein: a rectangular fence structure is arranged in the droplet capturing structure and is used for capturing droplets and blocking the droplets.
5. The integrated microfluidic chip with double T-shaped generating channels according to claim 4, wherein: the width of the mutual gap of the rectangular fence structures in the droplet capture structure is Ws, and the diameter d of the droplet is smaller than 0.25 d.
6. A microfluidic chip assembly, comprising: the dual-T-generation channel integrated microfluidic chip structure of claim 1, an oil phase inlet, a water phase inlet, and a waste liquid outlet; the oil phase inlet and the water phase inlet are connected with the double-T-shaped generation micro-channel structure, and the waste liquid cavity is connected with the micro-droplet capturing structure; the oil phase inlet is used for introducing liquid oil, the water phase inlet is used for introducing a sample, and the waste liquid outlet is used for leading out waste liquid.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120575667.XU CN214881407U (en) | 2021-03-19 | 2021-03-19 | double-T-shaped generation channel integrated micro-fluidic chip and micro-fluidic chip assembly |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120575667.XU CN214881407U (en) | 2021-03-19 | 2021-03-19 | double-T-shaped generation channel integrated micro-fluidic chip and micro-fluidic chip assembly |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214881407U true CN214881407U (en) | 2021-11-26 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120575667.XU Expired - Fee Related CN214881407U (en) | 2021-03-19 | 2021-03-19 | double-T-shaped generation channel integrated micro-fluidic chip and micro-fluidic chip assembly |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214881407U (en) |
-
2021
- 2021-03-19 CN CN202120575667.XU patent/CN214881407U/en not_active Expired - Fee Related
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| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211126 |