CN214830413U

CN214830413U - Reactor with a reactor shell

Info

Publication number: CN214830413U
Application number: CN202120647109.XU
Authority: CN
Inventors: 董永聘; 桑伟建; 冯荣
Original assignee: Fuyuan Shanghai Biotechnology Co ltd
Current assignee: Fuyuan Shanghai Biotechnology Co ltd
Priority date: 2021-03-30
Filing date: 2021-03-30
Publication date: 2021-11-23
Anticipated expiration: 2031-03-30

Abstract

The utility model discloses a reactor. In the utility model, a culture cavity for accommodating cells and a pair of liquid passing cavities communicated with the culture cavity are arranged in the reactor; the two liquid passing cavities are respectively positioned at any two sides of the culture cavity, and the liquid passing cavities are in butt joint communication with the opposite sides of the culture cavity; wherein, be equipped with filtration membrane respectively between two cross the sap cavity and the cultivation chamber, and filtration membrane is used for hindering the cell to get into and crosses the sap cavity. Compared with the prior art, the method can regulate the polarity of cells in the tissue organ culture process.

Description

Reactor with a reactor shell

Technical Field

The utility model relates to the field of biotechnology, in particular to reactor.

Background

Cell culture process the problem of seed cell polarity is the key to in vitro tissue organ and organoid culture. Currently, the methods adopted for in vitro organoid and tissue organ culture include suspension culture, matrigel culture, three-dimensional scaffold culture, and 3D printing. In vitro organ and tissue organ culture, cells need to be subjected to polarity construction according to the functions of the organs, but the existing culture only solves the problems of similarity in shape, disordered cell polarity and incapability of polarity control, and subsequent function limitation is directly caused.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a reactor for in tissue organ culture process, the polarity of adjustable cell.

In order to solve the technical problem, an embodiment of the present invention provides a reactor, wherein a culture chamber for accommodating cells and a pair of liquid passing chambers communicated with the culture chamber are formed in the reactor; the two liquid passing cavities are respectively positioned at any two sides of the culture cavity, and the liquid passing cavities are in butt joint communication with the opposite sides of the culture cavity; and filtering membranes are respectively arranged between the two liquid passing cavities and the culture cavity and are used for blocking the cells from entering the liquid passing cavities.

In one embodiment, the two liquid passing cavities are oppositely arranged.

In one embodiment, the extending directions of the two liquid passing cavities are crossed.

In one embodiment, the reactor comprises:

the culture plate is internally provided with the culture cavity;

a pair of cover plates, both of which hold the culture plate; the pair of liquid passing cavities are respectively arranged in the pair of cover plates; and the filtering membrane is arranged between each cover plate and the culture plate;

the liquid passing cavity is opened towards one side of the culture plate; the culture cavity is opened towards one side of the cover plate; wherein the part of the culture chamber opened towards one side of the cover plate is aligned with the part of the transliquid chamber opened towards one side of the culture plate.

In one embodiment, the radial cross section of the transliquid chamber in at least one of the cover plates is the same as the radial cross section of the culture chamber.

In one embodiment, the liquid passing cavity is provided with a plurality of liquid passing sub-cavities, and any two adjacent liquid passing sub-cavities are communicated; the culture cavity is provided with a plurality of culture sub-cavities, and two culture sub-cavities which are adjacent randomly are communicated;

wherein, cross the liquid sub-chamber with cultivate sub-chamber quantity the same, and a plurality of cultivate sub-chamber respectively with a plurality of cross the liquid sub-chamber and aim at the switch-on.

In one embodiment, the liquid passing sub-cavities are vertical channels arranged in sequence, and any two adjacent liquid passing sub-cavities are communicated end to end; the culture sub-cavities are vertical channels which are arranged in sequence, and any two adjacent culture sub-cavities are communicated end to end.

In one embodiment, the liquid passing subchambers are annular channels, and the annular channels are arranged radially.

In one embodiment, the reactor is provided with a culture inlet and a culture outlet which are communicated with the culture cavity.

In one embodiment, the reactor is provided with a liquid passing inlet and a liquid passing outlet which are communicated with the liquid passing cavities.

The utility model discloses embodiment is for prior art, owing to be equipped with culture chamber and a pair of sap cavity of crossing, and cross the sap cavity and be located the arbitrary both sides of cultivateing the chamber respectively, places the cell in the cultivation chamber, can let in different culture medium etc. in two crossing the sap cavity, cross the sap cavity and cultivate the chamber opposite side butt joint intercommunication, the culture medium of crossing in the sap cavity sees through filtration membrane and produces the influence to the cell, and the difference of cultivateing the factor impels the passive regulation space polarity structure of cell to reach and regulate and control cell polarity. And the filtering membrane between the liquid passing cavity and the culture cavity can ensure that the cells are stably positioned in the culture cavity for culture and cannot reach the liquid passing cavity.

Drawings

One or more embodiments are illustrated by way of example in the accompanying drawings, which correspond to the figures in which like reference numerals refer to similar elements and which are not to scale unless otherwise specified.

FIG. 1 is a schematic diagram of a reactor according to an embodiment of the present invention;

FIG. 2 is an exploded view of a reactor according to an embodiment of the present invention;

FIG. 3 is an exploded view of another perspective of a reactor according to an embodiment of the present invention;

FIG. 4 is a cross-sectional view of a reactor according to an embodiment of the present invention;

FIG. 5 is a schematic diagram of the reactor according to another embodiment of the present invention;

FIG. 6 is an exploded view of a reactor according to another embodiment of the present invention;

fig. 7 is a schematic structural view of a reactor cover plate according to another embodiment of the present invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the following will explain in detail each embodiment of the present invention with reference to the accompanying drawings. However, it will be appreciated by those of ordinary skill in the art that in various embodiments of the invention, numerous technical details are set forth in order to provide a better understanding of the present application. However, the technical solution claimed in the present application can be implemented without these technical details and various changes and modifications based on the following embodiments.

In the following description, for the purposes of illustrating various disclosed embodiments, certain specific details are set forth in order to provide a thorough understanding of the various disclosed embodiments. One skilled in the relevant art will recognize, however, that the embodiments may be practiced without one or more of the specific details. In other instances, well-known devices, structures and techniques associated with this application may not be shown or described in detail to avoid unnecessarily obscuring the description of the embodiments.

Throughout the specification and claims, the word "comprise" and variations thereof, such as "comprises" and "comprising," are to be understood as an open, inclusive meaning, i.e., as being interpreted to mean "including, but not limited to," unless the context requires otherwise.

Various embodiments of the present invention will be described in detail below with reference to the accompanying drawings so that the objects, features and advantages of the invention can be more clearly understood. It should be understood that the embodiments shown in the drawings are not intended as limitations on the scope of the invention, but are merely illustrative of the true spirit of the technical solution of the invention.

Reference throughout this specification to "one embodiment" or "an embodiment" means that a particular feature, structure, or characteristic described in connection with the embodiment is included in at least one embodiment. Thus, the appearances of the phrases "in one embodiment" or "in an embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, the particular features, structures, or characteristics may be combined in any suitable manner in one or more embodiments.

As used in this specification and the appended claims, the singular forms "a", "an", and "the" include plural referents unless the context clearly dictates otherwise. It should be noted that the term "or" is generally employed in its sense including "and/or" unless the context clearly dictates otherwise.

In the following description, for the sake of clarity, the structure and operation of the present invention will be described with the aid of directional terms, but the terms "front", "rear", "left", "right", "outer", "inner", "outer", "inward", "upper", "lower", etc. should be understood as words of convenience and not as words of limitation.

The reactor can be used for in vitro culture of tissues and organs, such as liver, kidney, pancreas, lung, etc.

Embodiments of the present invention are described below with reference to the drawings. As shown in FIGS. 1, 2, 3 and 4, a culture chamber 10 for accommodating cells and a pair of through

liquid chambers

20 and 30 communicating with the culture chamber 10 are formed in the reactor 100. The two liquid passing

cavities

20 and 30 are respectively positioned at any two sides of the culture cavity 10, the liquid passing cavity 20 is in butt joint communication with the opposite side of the culture cavity 10, and one side of the liquid passing cavity 30 facing the culture cavity 10 is in butt joint communication with one side of the culture cavity 10 facing the liquid passing cavity. Wherein, cross and be equipped with filtration membrane 4 between sap cavity 20 and the cultivation chamber 10, cross and be equipped with filtration membrane 5 between sap cavity 30 and the cultivation chamber 10, and

filtration membrane

4 and 5 are used for obstructing the cell to get into and cross the sap cavity. The thickness of the culture chamber 10 starts from the size of a single cell and can be in the order of millimeters to accommodate multiple layers of cells. The liquid passing cavity is a liquid flowing structure which can flow but is not limited to culture liquid, blood, gas and the like.

In cell culture, culture medium can be continuously introduced into the liquid passing

cavities

20 and 30 and is diffused into the culture cavity 10 through the

filter membranes

4 and 5, so that continuous liquid supply and liquid change in the culture cavity 10 are realized, oxygen and nutrient are more sufficiently supplied, and waste is metabolized. In order to adjust the polarity of the cells, different signals, such as blood, can be added to the culture medium, and the cells can adjust the polarity according to different signals from different directions. The filtering membrane can be a mesh screen, and the specification is 500 meshes to 10000 meshes, preferably 3000 meshes to 8000 meshes; more preferably 4000 to 6000 mesh. The

filtration membranes

4 and 5 are used for short-term or permanent isolation of cells, exchange of liquids, and the constituent materials include, but are not limited to, plastics, biodegradable materials, and the like, and for exchange, not limited to culture liquids, gases, and the like. The culture medium is continuously introduced into the liquid passing cavity,

as can be seen from the above, the culture chamber 10 and the pair of

liquid passing chambers

20 and 30 are provided, and the

liquid passing chambers

20 and 30 are respectively located at any two sides of the culture chamber 10, cells are placed in the culture chamber 10, different culture mediums can be introduced into the two

liquid passing chambers

20 and 30, the

liquid passing chambers

20 and 30 are communicated with the opposite sides of the culture chamber 10 in a butt joint manner, the culture mediums in the

liquid passing chambers

20 and 30 penetrate through the filter membranes to affect the cells, and the difference of culture factors promotes the cells to passively adjust the space polarity structure, so as to achieve the purpose of adjusting and controlling the cell polarity. And the

filter membranes

4 and 5 can allow the cells to be stably cultured in the culture chamber 10 without going into the

liquid passing chambers

20 and 30.

Further, as shown in fig. 1, 2, 3 and 4, the two

liquid passing chambers

20 and 30 are oppositely disposed. The two liquid passing

cavities

20 and 30 extend in parallel, and the culture cavity 10 is positioned between the two liquid passing

cavities

20 and 30 to separate the two liquid passing

cavities

20 and 30.

As shown in fig. 1, 2, 3 and 4, the reactor 100 includes: the culture plate 1, the

cover plates

2 and 3, and a culture cavity 10 is arranged in the culture plate 1. The

cover plates

2 and 3 hold the culture plate 1, half of the culture plate 1 is embedded in the cover plate 2, and half is embedded in the cover plate 3, and the

cover plates

2 and 3 cover the culture plate 1 after being butted. The liquid passing cavity 20 is arranged in the cover plate 2, and the liquid passing cavity 30 is arranged in the cover plate 3. And be equipped with filtration membrane 4 between apron 2 and culture plate 1, filtration membrane 4 centre gripping is between apron 2 and culture plate 1, covers the one side that crosses sap cavity 20 towards cultivateing chamber 10, and filtration membrane 4 can be pasted fixedly with apron 2 and culture plate 1. Be equipped with filtration membrane 5 between apron 3 and culture plate 1, filtration membrane 5 centre gripping is between apron 3 and culture plate 1, covers the one side of crossing sap cavity 30 orientation cultivation chamber 10, and filtration membrane 5 can be pasted fixedly with apron 3 and culture plate 1. The liquid passing chamber 20 is opened to the side of the culture plate 1, the culture chamber 10 is opened to the side of the cover plate 2, and the part of the culture chamber 10 opened to the side of the cover plate 2 is aligned with the part of the liquid passing chamber 20 opened to the side of the culture plate 1. The liquid passing chamber 30 is opened to the side of the culture plate 1, the culture chamber 10 is opened to the side of the cover plate 3, and the part of the culture chamber 10 opened to the side of the cover plate 3 is aligned with the part of the liquid passing chamber 30 opened to the side of the culture plate 1. It will be understood that the

cover plates

2 and 3 can be arranged opposite one another and parallel to one another, so that the two liquid-passing

chambers

20 and 30 can be arranged opposite one another and parallel to one another. The two cover plates can also be arranged left and right, or one is arranged on the left side and the other is arranged on the upper side, namely the arrangement direction of the two cover plates can have various forms and is not limited to be arranged opposite to the upper side and the lower side. That is, the liquid passing cavities in the two cover plates are also matched with the cover plates in various ways, and are not limited to the upper and lower layout in the drawing. The two liquid passing cavities can intersect in the extending direction, that is, one liquid passing cavity is arranged above the culture cavity 10, the other liquid passing cavity is arranged on the side of the culture cavity 10, and the layout form of the two liquid passing cavities has other various possibilities, which are not illustrated herein.

Further, as shown in FIG. 1, FIG. 2, FIG. 3 and FIG. 4, the radial section of the liquid passing chamber 20 in the cover plate 2 is the same as the radial section of the culture chamber 10, and the top of the sidewall of the liquid passing chamber 20 is just butted against the top of the sidewall of the culture chamber 10. Thereby allowing the cells in the culture chamber 10 to be in sufficient contact with the culture medium in the transliquid chamber 20. The radial cross section of the transliquid chamber 20 may be larger or smaller than the radial cross section of the culture chamber 10.

Further, as shown in FIG. 1, FIG. 2, FIG. 3 and FIG. 4, the radial section of the passage chamber 30 in the cover plate 3 is the same as the radial section of the culture chamber 10, so that the cells in the culture chamber 10 can be brought into sufficient contact with the culture medium in the passage chamber 30.

As shown in FIGS. 1, 2, 3 and 4, the cover plate 2, the cover plate 3 and the culture plate 1 may be fitted to each other in various ways, and the culture plate 1 may be embedded in the cover plate 2 and the cover plate 3 or may be fixed without being directly adhered or fixed in other ways.

In one embodiment, as shown in FIGS. 2, 3 and 4, the radial cross section of the passing lumen 20 and the radial cross section of the passing lumen 30 are the same as the radial cross section of the culture lumen 10, or one of the radial cross section of the passing lumen 20 and the radial cross section of the passing lumen 30 is the same as the radial cross section of the culture lumen 10.

Further, as shown in fig. 2 and 4, the liquid passing cavity 20 has a plurality of liquid passing sub-cavities 201, and any two adjacent liquid passing sub-cavities 201 are communicated with each other. Culture chamber 10 has a plurality of culture subcavities 101, and two culture subcavities 101 that are arbitrarily adjacent communicate. Liquid passing subchambers 201 are the same as culture subchambers 101 in number, and the plurality of culture subchambers 101 are aligned and communicated with the plurality of liquid passing subchambers 201 respectively. The liquid passing subchambers 201 are vertical channels arranged in sequence, and any two adjacent liquid passing subchambers 201 are communicated end to end. The culture sub-cavities 101 are vertical channels arranged in sequence, and any two adjacent culture sub-cavities 101 are communicated end to end. As shown in fig. 3 and 4, the structure of the liquid passing chamber 30 is the same as that of the liquid passing chamber 20, and the structure of the liquid passing chamber 30 will not be described.

It will be appreciated that the

chambers

20, 30 could be a single unitary chamber rather than having multiple subchambers. The same is true of the culture chamber 10.

As shown in FIGS. 2, 3 and 4, the reactor 100 is provided with a culture inlet 11 and a culture outlet 12 communicating with the culture chamber 10. The culture inlet 11 and the culture outlet 12 are opened in the culture plate 1.

As shown in fig. 2, 3 and 4, the reactor 100 is provided with a liquid passing inlet and a liquid passing outlet which are communicated with the liquid passing chambers. As shown in the figure, the cover plate 2 is provided with a liquid inlet 21 and a liquid outlet 22, and the cover plate 3 is provided with a liquid inlet 31 and a liquid outlet 32.

In another embodiment, as shown in FIGS. 5, 6 and 7, the reactor 600 has a different shape from the reactor 100 and basically the same principle, and the reactor 600 has a culture chamber 60 for accommodating cells and a pair of through

liquid chambers

70 and 80 communicating with the culture chamber 60. The liquid passing cavity 70 and the liquid passing cavity 80 are positioned at two sides of the culture cavity 60 and are in butt joint communication with the culture cavity 60. A filtering membrane 92 is arranged between the liquid passing cavity 70 and the culture cavity 60, and a filtering membrane 91 is arranged between the liquid passing cavity 80 and the culture cavity 60. The docking of the liquid passing chamber 70, the liquid passing chamber 80 and the culture chamber 60 may be the same as the reaction chamber 100, and will not be described in detail.

As shown in FIGS. 5, 6 and 7, the reactor 600 has a culture plate 6,

cover plates

7 and 8, a culture chamber 60 is formed in the culture plate 6, a liquid passing chamber 70 is formed in the cover plate 7, and a liquid passing chamber 80 is formed in the cover plate 8. The reactor 600 is provided with a culture inlet 61 and a culture outlet 62 which are communicated with the culture chamber 60. The cover plate 7 is provided with a liquid inlet 71 and a liquid outlet 72, and the cover plate 8 is provided with a liquid inlet 81 and a liquid outlet 82. The liquid passing cavity 80 is provided with liquid passing sub-cavities 801, the liquid passing sub-cavities 801 are annular channels, and the annular channels are arranged in a radial direction. The liquid cavity 70 is provided with a liquid passing sub-cavity 701, the liquid passing sub-cavity 701 is an annular channel, and the annular channels are arranged in a radial direction. Culture chamber 60 has a culture subchamber 601, with culture subchamber 601 being an annular channel, and with each annular channel being radially aligned.

It is understood that the reactor may have various shapes and configurations, and the liquid passing chamber and the culture chamber in the reactor may be provided in various forms such as irregular extension or inclined extension, and the present invention is not limited to the above embodiments.

While the preferred embodiments of the present invention have been described in detail above, it should be understood that aspects of the embodiments can be modified, if necessary, to employ aspects, features and concepts of the various patents, applications and publications to provide yet further embodiments.

These and other changes can be made to the embodiments in light of the above detailed description. In general, in the claims, the terms used should not be construed to be limited to the specific embodiments disclosed in the specification and the claims, but should be construed to include all possible embodiments along with the full scope of equivalents to which such claims are entitled.

It will be understood by those skilled in the art that the foregoing embodiments are specific examples of the invention, and that various changes in form and details may be made therein without departing from the spirit and scope of the invention in its practical application.

Claims

1. A reactor is characterized in that a culture cavity for accommodating cells and a pair of liquid passing cavities communicated with the culture cavity are formed in the reactor; the two liquid passing cavities are respectively positioned at any two sides of the culture cavity, and the liquid passing cavities are in butt joint communication with the opposite sides of the culture cavity; and filtering membranes are respectively arranged between the two liquid passing cavities and the culture cavity and are used for blocking the cells from entering the liquid passing cavities.

2. The reactor of claim 1, wherein the two liquid passing chambers are oppositely arranged.

3. The reactor according to claim 1, wherein the two liquid passing chambers are crossed in the extending direction.

4. A reactor according to claim 3, characterized in that it comprises:

the culture plate is internally provided with the culture cavity;

5. A reactor according to claim 4, wherein the radial cross-section of the passing chamber in at least one of the cover plates is the same as the radial cross-section of the culture chamber.

6. The reactor according to claim 5, wherein the liquid passing cavity is provided with a plurality of liquid passing sub-cavities, and any two adjacent liquid passing sub-cavities are communicated; the culture cavity is provided with a plurality of culture sub-cavities, and two culture sub-cavities which are adjacent randomly are communicated;

7. The reactor of claim 6, wherein the liquid passing subchambers are vertical channels arranged in sequence, and any two adjacent liquid passing subchambers are communicated end to end; the culture sub-cavities are vertical channels which are arranged in sequence, and any two adjacent culture sub-cavities are communicated end to end.

8. The reactor of claim 6 wherein said weep subchambers are annular channels and each of said annular channels is radially aligned.

9. The reactor according to claim 1, wherein the reactor is provided with a culture inlet and a culture outlet which are communicated with the culture cavity.

10. The reactor of claim 1, wherein the reactor is provided with a liquid passing inlet and a liquid passing outlet which are communicated with the liquid passing cavities.