CN216972485U - Biological culture device - Google Patents

Abstract

The utility model discloses a biological culture device, which comprises an upper cover, a lower cover and at least one culture main body, wherein the at least one culture main body is positioned between the upper cover and the lower cover, the culture main body comprises a perfusion layer, a culture flow channel layer and a separation layer, the separation layer is positioned between the perfusion layer and the culture flow channel layer, the culture flow channel layer is provided with a culture flow channel with a first preset flow channel trend, and a culture solution in the perfusion layer permeates and/or circulates to the culture flow channel layer through the separation layer.

Description

Biological culture device

Technical Field

The utility model relates to the technical field of biomedicine, in particular to a biological culture device.

Background

Biological culture is a basic experimental means for various researches in life science, and makes great contribution to biological researches in various fields for a long time. However, with the development and expansion of technology, there is a higher demand for biological culture in both industrial applications and research, for example, a larger-scale biological culture apparatus, a more efficient culture method, and the like are required.

It is therefore desirable to provide a biological growth apparatus that can fulfill the needs for larger scale, more rational structural arrangements, and more efficient biological growth.

SUMMERY OF THE UTILITY MODEL

One embodiment of the present disclosure provides a biological growth apparatus. Biological culture apparatus includes upper cover, lower cover and at least one cultivation main part, at least one cultivation main part is located the upper cover between the lower cover, cultivate the main part including perfusion layer, cultivation runner layer and separate layer, the separate layer is located perfusion layer with cultivate between the runner layer, cultivate the runner layer and have the cultivation runner of first predetermined runner trend, the culture solution in the perfusion layer passes through separate layer infiltration and/or circulation extremely cultivate in the runner layer.

In some embodiments, at least one of the upper cover, the lower cover and the perfusion layer has at least one perfusion inlet and/or at least one of the upper cover, the lower cover and the perfusion layer has at least one perfusion outlet.

In some embodiments, the culture flow channel layer and/or the spacer layer has a communication channel that communicates with the perfusion inlet and/or perfusion outlet.

In some embodiments, the culture body comprises the culture flow channel layer, two perfusion layers, and two separation layers, the culture flow channel layer is located between the two separation layers, and the two perfusion layers are located at upper and lower layers of the two separation layers, respectively.

In some embodiments, the perfusion layer has at least one perfusion chamber and/or at least one perfusion flow channel.

In some embodiments, the perfusion chamber is a hollow cavity structure surrounded by a chamber outer wall and/or the perfusion channel has a second predetermined flow channel course.

In some embodiments, the first predetermined flow path comprises at least one of a spiral bow, a spiral loop, a spiral bend, a spiral fold, and/or the second predetermined flow path comprises at least one of a spiral bow, a spiral loop, a spiral bend, a spiral fold.

In some embodiments, the perfusion channel is a hollow channel structure surrounded by perfusion channel walls.

In some embodiments, the culture flow channel is a hollow flow channel structure surrounded by culture flow channel walls.

In some embodiments, the culture flow channel layer has at least one culture flow channel inlet and at least one culture flow channel outlet.

Drawings

The present description will be further explained by way of exemplary embodiments, which will be described in detail by way of the accompanying drawings. These embodiments are not intended to be limiting, and in these embodiments like numerals are used to indicate like structures, wherein:

FIG. 1 is a schematic perspective view of a biological growth device 100 according to some embodiments of the disclosure;

FIG. 2 is a schematic illustration of an exploded view of biological growth apparatus 100, according to some embodiments herein;

FIG. 3A is a schematic view of the structural composition of an infusion layer according to some embodiments herein;

FIG. 3B is a schematic diagram illustrating a structural composition of an infusion layer according to still other embodiments of the present disclosure;

FIG. 4A is a schematic illustration of a perfusion layer or culture channel layer according to some embodiments described herein;

FIG. 4B is a schematic diagram of a perfusion layer or culture channel layer according to further embodiments of the present disclosure;

FIG. 4C is a schematic diagram of a perfusion layer or culture channel layer according to further embodiments of the present disclosure;

FIG. 4D is a schematic diagram of a perfusion layer or culture channel layer according to further embodiments of the present disclosure;

FIG. 5 is an exploded schematic view of a biological growth device 500 according to some embodiments of the disclosure.

FIG. 6 is an exploded schematic view of a biological growth device 600 according to some embodiments of the disclosure.

Detailed Description

In order to more clearly illustrate the technical solutions of the embodiments of the present disclosure, the drawings used in the description of the embodiments will be briefly described below. It is obvious that the drawings in the following description are only examples or embodiments of the present description, and that for a person skilled in the art, the present description can also be applied to other similar scenarios on the basis of these drawings without inventive effort. Unless otherwise apparent from the context, or otherwise indicated, like reference numbers in the figures refer to the same structure or operation.

As used in this specification and the appended claims, the terms "a," "an," "the," and/or "the" are not to be taken in a singular sense, but rather are to be construed to include a plural sense unless the context clearly dictates otherwise. In general, the terms "comprises" and "comprising" merely indicate that steps and elements are included which are explicitly identified, that the steps and elements do not form an exclusive list, and that a method or apparatus may include other steps or elements.

Flow charts are used in this description to illustrate operations performed by a system according to embodiments of the present description. It should be understood that the preceding or following operations are not necessarily performed in the exact order in which they are performed. Rather, the various steps may be processed in reverse order or simultaneously. Meanwhile, other operations may be added to the processes, or a certain step or several steps of operations may be removed from the processes.

The biological culture apparatus provided in the embodiments of the present specification will be described in detail below with reference to the drawings.

FIG. 1 is a schematic perspective view of a biological growth apparatus 100, according to some embodiments herein. FIG. 2 is a schematic diagram of an exploded view of biological growth device 100, as shown in some embodiments of the present disclosure. FIG. 3A is a schematic illustration of a structural composition of an infusion layer, in accordance with certain embodiments of the present disclosure; fig. 3B is a schematic structural composition diagram of a perfusion layer according to further embodiments of the present disclosure. FIG. 4A is a schematic illustration of a perfusion layer or culture channel layer according to some embodiments of the present disclosure; FIG. 4B is a schematic diagram of a perfusion layer or culture channel layer according to further embodiments of the present disclosure; FIG. 4C is a schematic diagram of a perfusion layer or culture channel layer according to further embodiments of the present disclosure; FIG. 4D is a schematic diagram of a perfusion layer or culture channel layer according to further embodiments of the present disclosure.

As shown in fig. 1 and 2, the biological growth device 100 may include an upper cap 110, a lower cap 150, and at least one growth body. The culture main body is located between the upper cover 110 and the lower cover 150, and as a core part for performing biological culture, the culture main body may include a perfusion layer 120, a separation layer 130 and a culture flow channel layer 140, wherein the separation layer 130 is located between the perfusion layer 120 and the culture flow channel layer 140, the culture flow channel layer 140 has a culture flow channel with a first predetermined flow channel direction, and the perfusion layer 120 permeates and/or circulates a culture solution into the culture flow channel layer 140 through the separation layer 130.

Specifically, biological culture apparatus 100 is an apparatus for culturing biological tissue. In some embodiments, biological culture device 100 can be used to culture cells, such as two-dimensional (2D) or three-dimensional (3D) cell cultures. In some embodiments, biological culture device 100 can be used to culture organoids. In some embodiments, biological culture device 100 can be used to culture adherent cells. In some embodiments, biological culture device 100 can be used to culture suspended cells or cell masses. In some embodiments, biological culture device 100 can be used to culture gel-encapsulated cell masses. It should be noted that the biological culture apparatus 100 can also be used for culturing any other type of possible biological tissue, and the present specification is not particularly limited.

Upper cover 110 and lower cover 150 are located at the top and bottom of biological growth device 100, respectively, to isolate the growth body from the external environment. The upper and lower covers 110 and 150 can prevent both external bacteria or microorganisms from contaminating organisms cultured in the organism culturing apparatus 100 and foreign substances (e.g., dust, etc.) in the external environment from entering the culture main body, thereby avoiding contamination of the environment in which the organisms are cultured. In some embodiments, the upper cover 110 and the lower cover 150 can be interchanged, and after the interchange, the lower cover 150 plays a role of the upper cover 110, and the upper cover 110 plays a role of the lower cover 150. In some embodiments, the upper cover 110 and the lower cover 150 may have the same structure (e.g., plate shape, cover with cavity, etc.) and/or material (e.g., polymer material, glass, etc.). In some embodiments, the upper cover 110 and the lower cover 150 may be made of different structures and/or materials. In some embodiments, the upper cover 110 and the lower cover 150 may be made of odorless, nontoxic, heat-resistant, and corrosion-resistant polymer material, such as polypropylene, polystyrene, polycarbonate, polymethyl methacrylate, and the like. In some embodiments, the upper cover 110 and the lower cover 150 can be sterilized with steam to ensure a satisfactory, sterile environment within the biological growth device 100. In some embodiments, the upper cover 110 and the lower cover 150 may be made of glass, which has good corrosion resistance and light transmittance, so as to meet practical requirements and facilitate observation of the biological culture from the outside. It should be noted that the upper cover 110 and the lower cover 150 may be made of any other possible structural form or material, and the present specification is not particularly limited.

In some embodiments, the top cover 110 may be provided with a perfusion inlet (e.g., perfusion inlet 110a or perfusion inlet 110b) for injecting or placing nutrients (nutrient solution, etc.) for biological cultivation. In some embodiments, two or more filling inlets may be disposed on the upper cover 110, such as including the filling inlet 110a and the filling inlet 110 b. In some embodiments, a pour outlet may be provided in the lower cap 150 for discharging nutrients or nutrient waste, such as the pour outlet 150 a. In some embodiments, two or more perfusion outlets may be provided on the lower cover 150, including, for example, perfusion outlet 150a and perfusion outlet 150 b. The upper cover 110 and/or the lower cover 150 are provided with a plurality of filling inlets and/or a plurality of filling outlets, so that the filling efficiency and the filling convenience are improved.

In some embodiments, the irrigation outlet of the lower cap 150 may serve as an irrigation inlet. The pouring inlet of the upper cap 110 may serve as a pouring outlet for assuming the function of the pouring outlet. That is to say, when carrying out the pouring or putting into of nutrient substance, according to actual need and concrete control mode, both can pour into or put into from the top down, also can pour into or put into from the bottom up, can also carry out above-mentioned two processes simultaneously to multiple nutrient substance when satisfying biological cultivation lets in the demand, improves and fills efficiency, optimizes the filling performance.

In some embodiments, the filling inlet and the filling outlet may be both provided on the upper cap 110 or the lower cap 150, for example, both the filling inlet and the filling outlet are provided on the upper cap 110. In some embodiments, the filling inlet and the filling outlet may be separately provided on the upper cap 110 or the lower cap 150, for example, the filling inlet is provided on the upper cap 110, and the filling outlet is provided on the lower cap 150.

The filling layer 120 is used for containing or temporarily storing nutrients, such as nutrient solution.

In some embodiments, perfusion layer 120 may have at least one perfusion chamber (e.g., perfusion chamber 610 in fig. 6) and/or at least one perfusion channel (e.g., perfusion channel on perfusion layer 120 in fig. 2).

In some embodiments, the perfusion layer 120 may be provided with a plurality of perfusion chambers, such as perfusion chamber 311 and perfusion chamber 312 in perfusion layer 310 of fig. 3A, so as to accommodate different nutrient solutions in different regions, so as to meet different biological needs, improve the adaptability of the biological culture apparatus 100, and optimize the practical performance of biological culture.

In some embodiments, the perfusion layer 120 may be a combined structure of a perfusion chamber and a perfusion channel, for example, the perfusion layer 320 in fig. 3B includes a perfusion chamber 321 and a perfusion channel 322.

In some embodiments, the perfusion chamber is a hollow cavity structure surrounded by the outer wall of the chamber, and when the separation layer 130 disposed adjacent to the perfusion layer 120 is a permeable functional layer, this structure can ensure good permeability.

In some embodiments, the perfusion channel has a second predetermined flow path orientation of the perfusion channel. The second preset flow channel trend is the preset flow channel trend in the perfusion layer, and the flow channel structure arrangement is convenient for controlling perfusion flow rate according to needs and can realize more reasonable perfusion flow channel space configuration. In some embodiments, the second predetermined flow path comprises at least one of a spiral bow, a spiral loop, a spiral bend, a spiral dogleg, and the like. For example, as shown in fig. 4A, the second predetermined flow path of the perfusion layer 410 may have a spiral bow shape. For example, as shown in fig. 4B, the second predetermined flow path of the perfusion layer 420 may have a spiral shape. For another example, as shown in fig. 4C, the second predetermined flow path of the perfusion layer 430 may have a spiral curvature. For another example, as shown in fig. 4D, the second predetermined flow path of the perfusion layer 440 may be a spiral fold. It should be noted that the second predetermined flow path orientation may also include other orientations, such as a zigzag, etc.

Generally, the perfusion cavity can realize relatively high perfusion flow rate, perfusion is more direct, the perfusion flow channel has the advantages of being capable of controlling perfusion volume and uniform flow rate, and various combination settings can be carried out according to different requirements of cultured organisms so as to improve perfusion efficiency and better meet perfusion requirements.

In some embodiments, at least one perfusion inlet and at least one perfusion outlet may be disposed on the perfusion layer 120, such as perfusion inlet 120a and perfusion outlet 120b shown in fig. 2. In some embodiments, nutrients may be introduced directly from the perfusion inlet 120a and expelled from the perfusion outlet 120 b.

The separation layer 130 is located between the perfusion layer 120 and the culture channel layer 140, and serves to separate the perfusion layer 120 from the culture channel layer 140. In some embodiments, a separation layer may employ a membrane layer having a specific osmotic function, such as an osmotic membrane that allows only fluid to pass through but not cells, such that nutrients may permeate through the osmotic membrane into culture channel layer 140. In some embodiments, the partition layer 130 and/or the culture flow channel layer 140 have communication channels, i.e., communication therebetween, and nutrients can be introduced into the culture flow channel layer 140 through the partition layer 130 via the communication channels, such as the communication hole 130a, the communication hole 130b, the culture flow channel inlet 140a, and the culture flow channel outlet 140b of fig. 2, by which communication with each other enables more convenient perfusion. In some embodiments, when the perfusion layer 120 is a permeable membrane layer, the separation layer 130 and the culture channel layer 140 may not be provided with a communication channel or may be partially provided with a communication channel, and nutrients may enter the culture channel layer 140 by the permeation action of the separation layer 130. In some embodiments, the separation layer 130 may be a permeable membrane layer, and one or more communication channels may be disposed on both the separation layer 130 and the culture channel layer 140, so as to further achieve a superior perfusion effect.

Culture channel layer 140 is a structure used in biological growth device 100 for growing organisms. In some embodiments, organisms may be cultured within culture channel layer 140. For example, cells can be cultured in the culture flow channel layer 140.

In some embodiments, culture flow channel layer 140 has a first predetermined flow channel orientation of culture flow channels. In some embodiments, the culture flow channel may be a hollow flow channel structure surrounded by culture flow channel walls. This structure can secure good permeability when the separation layer 130 disposed adjacent to the culture flow channel layer 140 employs a permeable functional layer. The first preset flow channel direction is the preset flow channel direction in the culture flow channel layer 140, and the structure arrangement not only can fully utilize the internal space of the culture flow channel layer to enlarge the scale of cultured organisms to a great extent, but also can be convenient for controlling the conveying of the cultured organisms in the culture flow channel, and improve the organism culture efficiency. In some embodiments, the first predetermined flow path comprises at least one of a spiral bow, a spiral meander, a spiral bend, and a spiral dogleg. In some embodiments, the first predetermined flow path orientation may also include other orientations, such as a zigzag, etc.

In some embodiments, the first predetermined flow path and the second predetermined flow path may be identical in the same biological growth device 100. In some embodiments, the first predetermined flow path and the second predetermined flow path may not coincide exactly in the same biological growth device 100. At this time, the structures, specific positions and local directions of the culture flow channel inlet and the culture flow channel outlet in the direction of the first predetermined flow channel and the perfusion inlet and the perfusion outlet in the direction of the second predetermined flow channel can not be completely corresponding and consistent. For example, in FIG. 2, the first predetermined flow path orientation of culture flow path layer 140 does not completely coincide with the second predetermined flow path orientation of perfusion layer 120. The structure, specific position and local orientation of the perfusion outlet 120b of the perfusion layer 120 and the culture channel outlet 140b of the culture channel layer 140 may not be completely corresponding and consistent.

In some embodiments, culture flow channel layer 140 has at least one culture flow channel inlet and at least one culture flow channel outlet. For example, the culture flow path may have one culture flow path inlet and one culture flow path outlet, such as culture flow path inlet 140a, culture flow path outlet 140b in fig. 2. For another example, the culture flow path may have two culture flow path inlets and two culture flow path outlets.

In some embodiments, the number of culture flow channel inlets and the number of culture flow channel outlets may be the same. For example, a culture flow channel may have three culture flow channel inlets and three culture flow channel outlets. In some embodiments, the number of culture flow channel inlets and the number of culture flow channel outlets may be different. For example, a culture flow channel may have three culture flow channel inlets and two culture flow channel outlets.

In some embodiments, a culture can be placed into a culture flow channel through a culture flow channel inlet. For example, organoid stem cells to be cultured are placed into a culture flow channel through an inlet of the culture flow channel. In some embodiments, the culture flow channel may also be perfused with liquid through the culture flow channel inlet to flush organisms cultured in the culture flow channel out of the culture flow channel outlet. For example, when the cultured organism is adherent cells, the digestion solution is poured into the culture flow channel through the culture flow channel inlet 140a so that the adherent cells are exfoliated, and the culture medium is poured into the culture flow channel through the culture flow channel inlet 140a so that the suspension cells are flushed out from the culture flow channel outlet 140 b. For another example, when the cultured organism is a gel-encapsulated cell mass, the temperature of the biological culture apparatus 100 can be changed (e.g., increased or decreased) by an external means to liquefy the gel in the culture flow channel, and the culture medium can be perfused into the culture flow channel through the culture flow channel inlet 140a to flush the cell mass out of the culture flow channel outlet 140 b.

In some embodiments, one or more culture bodies may be provided as needed in order to further expand the culture scale.

FIG. 5 is an exploded schematic view of a biological growth device 500 according to some embodiments of the disclosure. FIG. 6 is an exploded schematic view of a biological growth device 600 according to some embodiments of the disclosure.

As shown in FIG. 5, biological growth device 500 includes, from top to bottom, top cover 110, perfusion layer 120, spacer layer 130, culture channel layer 140, spacer layer 130, perfusion layer 120, and bottom cover 150, respectively. Wherein the upper and lower perfusion layers 120 respectively include perfusion inlet ports 120a and perfusion outlet ports 120b, the culture channel layer 140 includes culture channel inlet ports 140a and culture channel outlet ports 140b, and the upper and lower separators 130 each include communication holes 130 a.

Specifically, the culture solution is poured into the pouring channel of the upper pouring layer 120 through the pouring inlet 120a, and then flows into the culture channel of the culture channel layer 140 through the communication hole 130a in the upper partition layer 130 to supply nutrients to the culture, and the surplus or waste culture solution flows into the lower pouring layer 120 through the communication hole 130a in the lower partition layer 130, and finally flows out from the pouring outlet 120b of the lower pouring layer 120. Wherein the culture in the culture flow channel layer 140 can be flushed out of the culture flow channel outlet 140b by pouring liquid into the culture flow channel through the culture flow channel inlet 140 a.

The biological culture apparatus 500 can make the culture solution more sufficiently contact with the culture in the culture channel layer 140 by setting the upper and lower perfusion layers 120, so that the culture grows uniformly. In addition, the upper and lower perfusion layers 120 are set as perfusion channels, so that the flow rate of the perfusion culture solution can be more uniform, and the condition that the culture structure is damaged due to too high flow rate can be avoided. The perfusion amount of the culture solution can be relatively reduced, and the waste is reduced.

As shown in FIG. 6, biological growth apparatus 600 includes, from top to bottom, top cap 110, perfusion layer 610, separation layer 130, culture flow channel layer 140, separation layer 130, perfusion layer 120, and bottom cap 150, respectively. Wherein the first perfusion layer 610 includes a perfusion inlet 610a, the upper and lower culture channel layers 140 include culture channel inlets 140a and culture channel outlets 140b, the third and fourth partition layers 130 include communication holes 130a, and the perfusion layer 120 includes a perfusion outlet 120 b.

Specifically, the culture solution is poured into the pouring chamber of the first pouring layer 610 through the pouring inlet 610a, and then permeates into the culture flow channel of the culture flow channel layer 140 through the first partition layer 130 to supply the culture nutrient, the culture solution in the culture flow channel layer 140 permeates into the pouring layer 610 through the second partition layer 130, and then flows into the culture flow channel of the second culture flow channel layer 140 through the communication hole 130a in the third partition layer 130 to supply the culture nutrient, and the surplus or waste culture solution flows into the lower pouring layer 120 through the communication hole 130a in the fourth partition layer 130, and finally flows out from the pouring outlet 120b of the lower pouring layer 120. The culture in the upper and lower culture channel layers 140 can be flushed out from the culture channel outlet 140b by pouring liquid into the culture channel through the culture channel inlet 140 a.

The two layer culture flow channel layer 140 design of biological growth device 600 allows for the simultaneous growth of more cultures. And the perfusion layers 610 of the first and second layers adopt a perfusion chamber design that allows the upper hydraulic pressure to increase to allow the culture fluid to permeate downward through the separation membranes 130 of the first and second layers. The separation membranes 130 of the third and fourth layers can smoothly flow down even when the culture solution is collected by adopting the design of the communication hole 130a so that the lower fluid pressure is reduced.

It should be noted that in the present specification, the structural shapes of the perfusion inlet, the perfusion outlet, the perfusion flow channel, the culture flow channel inlet, the culture flow channel outlet, the culture flow channel, and the communication holes, and the arrangement or layout positions thereof in the corresponding layers are merely exemplary in the drawings, and any other possible arrangement may be made without departing from the scope of the present invention.

Compared with the prior art, the biological culture device provided by the embodiment of the utility model has the following beneficial effects that: (1) the culture flow channel of the culture flow channel layer can simultaneously realize sample introduction, culture and sampling, so that the complicated design of an inlet, an outlet, a pipeline and a culture chamber is avoided, and the structure is more reasonable; (2) the design of the culture flow channel layer can fully utilize the internal space of the culture flow channel layer to culture more biological tissues; (3) the design of the perfusion channel in the perfusion layer and the culture channel in the culture channel layer can reduce the using amount of the culture solution and avoid waste; (4) the repeated operation of pouring culture solution among different culture containers is reduced by arranging the multilayer culture flow passages to culture a plurality of groups of organisms at one time; (5) simple structure, convenient operation can only maintain and replace partial structure, and is with low costs. It is to be noted that different embodiments may produce different advantages, and in different embodiments, any one or combination of the above advantages may be produced, or any other advantages may be obtained.

Having thus described the basic concept, it will be apparent to those skilled in the art that the foregoing detailed disclosure is to be regarded as illustrative only and not as limiting the present specification. Various modifications, improvements and adaptations to the present description may occur to those skilled in the art, although not explicitly described herein. Such modifications, improvements and adaptations are proposed in the present specification and thus fall within the spirit and scope of the exemplary embodiments of the present specification.

Also, the description uses specific words to describe embodiments of the description. Reference throughout this specification to "one embodiment," "an embodiment," and/or "some embodiments" means that a particular feature, structure, or characteristic described in connection with at least one embodiment of the specification is included. Therefore, it is emphasized and should be appreciated that two or more references to "an embodiment" or "one embodiment" or "an alternative embodiment" in various places throughout this specification are not necessarily all referring to the same embodiment. Furthermore, some features, structures, or characteristics of one or more embodiments of the specification may be combined as appropriate.

Additionally, the order in which the elements and sequences of the process are recited in the specification, the use of alphanumeric characters, or other designations, is not intended to limit the order in which the processes and methods of the specification occur, unless otherwise specified in the claims. While various presently contemplated embodiments have been discussed in the foregoing disclosure by way of example, it should be understood that such detail is solely for that purpose and that the appended claims are not limited to the disclosed embodiments, but, on the contrary, are intended to cover all modifications and equivalent arrangements that are within the spirit and scope of the embodiments herein. For example, although the system components described above may be implemented by hardware devices, they may also be implemented by software-only solutions, such as installing the described system on an existing server or mobile device.

Similarly, it should be noted that in the preceding description of embodiments of the present specification, various features are sometimes grouped together in a single embodiment, figure, or description thereof for the purpose of streamlining the disclosure and aiding in the understanding of one or more of the embodiments. This method of disclosure, however, is not intended to imply that more features are required than are expressly recited in the claims. Indeed, the embodiments may be characterized as having less than all of the features of a single embodiment disclosed above.

Finally, it should be understood that the embodiments described herein are merely illustrative of the principles of the embodiments of the present disclosure. Other variations are also possible within the scope of the present description. Thus, by way of example, and not limitation, alternative configurations of the embodiments of the specification can be considered consistent with the teachings of the specification. Accordingly, the embodiments of the present description are not limited to only those embodiments explicitly described and depicted herein.

Claims (10)

1. A biological culture device, characterized in that, includes upper cover, lower cover and at least one cultivation main part, at least one cultivation main part is located the upper cover between the lower cover, cultivate the main part and include perfusion layer, cultivation runner layer and separate layer, the separate layer is located perfusion layer with cultivate between the runner layer, cultivate the runner layer and have the cultivation runner of first predetermined runner trend, culture solution in the perfusion layer passes through the separate layer infiltration and/or circulate to cultivate in the runner layer.

2. The biological cultivation device of claim 1 wherein at least one of the upper lid, the lower lid and the perfusion layer has at least one perfusion inlet and/or at least one of the upper lid, the lower lid and the perfusion layer has at least one perfusion outlet.

3. The biological cultivation device according to claim 2, wherein the cultivation channel layer and/or the separation layer has a communication channel which communicates with the perfusion inlet and/or the perfusion outlet.

4. The biological cultivation device of claim 1 wherein the cultivation body comprises the cultivation channel layer, two perfusion layers, two separation layers, the cultivation channel layer being located between the two separation layers, the two perfusion layers being located respectively on the upper and lower layers of the two separation layers.

5. The biological growth device of claim 1, wherein the perfusion layer has at least one perfusion chamber and/or at least one perfusion flow channel.

6. Biological cultivation device according to claim 5, characterised in that the perfusion chamber is a hollow cavity structure surrounded by a chamber outer wall and/or that the perfusion channel has a second predetermined flow path course.

7. The biological culture device of claim 6, wherein the first predetermined flow path comprises at least one of a spiral bow, a spiral loop, a spiral bend, and a spiral fold, and/or wherein the second predetermined flow path comprises at least one of a spiral bow, a spiral loop, a spiral bend, and a spiral fold.

8. The biological growth device of claim 6, wherein the perfusion channel is a hollow channel structure surrounded by perfusion channel walls.

9. The biological cultivation device according to any one of claims 1 to 8 wherein the cultivation flow channel is a hollow flow channel structure surrounded by a cultivation flow channel wall.

10. The biological growth device of claim 9, wherein the growth channel layer has at least one growth channel inlet and at least one growth channel outlet.

Images