CN214735698U - 384-hole fluorescent quantitative PCR plate ice box fixing device - Google Patents

Abstract

The utility model discloses a 384-hole fluorescence quantitative PCR plate ice box fixing device, which comprises a multipurpose rectangular ice box, wherein a flat-layer full clapboard is formed at the inner side of the multipurpose rectangular ice box, and the upper part of the clapboard and a box wall positioned above the clapboard are enclosed together to form a concave accommodating part for placing a 384-hole fluorescence quantitative PCR plate; the length of the upper surface of the partition board is larger than that of the 384-hole fluorescent quantitative PCR plate; a plate holder for filling a gap in a long direction between the 384-well fluorescent quantitative PCR plate and the multipurpose rectangular ice box. The application provides a 384 hole fluorescence ration PCR board ice box fixing device, multi-purpose rectangle ice box can laminate with 384 hole board bottom closely, has guaranteed low temperature and fixed effect, has promoted experimenter's operation accuracy and experimental speed to improve experimental efficiency. The plate support is also provided with a through hole with the aperture corresponding to that of the multipurpose rectangular ice box, so that the temporary storage reagent EP tube can be conveniently used.

Description

384-hole fluorescent quantitative PCR plate ice box fixing device

Technical Field

The utility model relates to an experimental facilities technical field especially relates to a 384 hole fluorescence ration PCR board ice chest fixing device.

Background

With the continuous progress of scientific research in China, the application of experimental technologies such as real-time fluorescent quantitative PCR, multi-plate cell culture, enzyme-linked immunosorbent assay and the like is more and more extensive. In performing the above experiments, 6-well plates, 12-well plates, 24-well plates, 48-well plates, 96-well plates, 384-well plates, etc. are often used, wherein real-time fluorescence quantitative PCR is one of the most commonly used experimental techniques in life science research, and 384-well fluorescence quantitative PCR plates are generally used when the amount of a sample to be detected is large, especially in a large number of clinical sample experiments.

The 384-hole fluorescence quantitative PCR plate is a microporous plate which is frequently used in biomedical technology experiments, and because the 384-hole fluorescence quantitative PCR plate is lighter, if the plate is not fixed in the using process, the movement of the plate is easy to occur, and the accuracy of experiment operation and sample adding is influenced. In the prior art, a 384-hole fluorescent quantitative PCR plate is usually arranged on a multipurpose rectangular ice box, and the inner diameter of the surface of the multipurpose rectangular ice box is 16cm due to the fixed size of the multipurpose rectangular ice box. The 384-well fluorescent quantitative PCR plate is 12.8cm in length, so that the 384-well fluorescent quantitative PCR plate is not matched with the multipurpose ice box in size in the length direction, the 384-well fluorescent quantitative PCR plate is easy to sideslip left and right when being loaded on the ice box in the loading process, and the difficulty of hole position positioning loading in the experimental process is increased.

SUMMERY OF THE UTILITY MODEL

The utility model provides a 384 hole fluorescence quantitative PCR board ice box fixing device.

The utility model provides a following scheme:

a384-well fluorescent quantitative PCR plate ice box fixing device comprises:

the multifunctional rectangular ice box is characterized in that a flat-layer full clapboard is formed on the inner side of the multifunctional rectangular ice box, and the upper part of the clapboard and the box wall positioned above the clapboard jointly enclose a concave accommodating part for placing a 384-hole fluorescent quantitative PCR plate; the length of the upper surface of the partition board is larger than that of the 384-hole fluorescent quantitative PCR plate;

a plate holder for filling a gap in a longitudinal direction between the 384-well fluorogenic quantitative PCR plate and the multipurpose rectangular ice box so as to restrict a movement of the 384-well fluorogenic quantitative PCR plate in the longitudinal direction with respect to the partition.

Preferably: one side surface of the flat plate support is used for being abutted against the partition plate, and two vertical surfaces in the width direction of the flat plate support are respectively used for being abutted against one vertical surface in the length direction of the 384-hole fluorescent quantitative PCR plate and one vertical surface in the length direction of the multipurpose rectangular ice box.

Preferably: the length of the plate holder is the same as the width of the interior of the concave receiving part, and the difference between the width of the plate holder and the length of the interior of the concave receiving part is the same as the length of the 384-well fluorescent quantitative PCR plate.

Preferably: the partition plate is provided with a first through hole part and a second through hole part, the first through hole part comprises 384 first through holes corresponding to 384 holes on the 384-hole fluorescent quantitative PCR plate, and the second through hole part comprises a plurality of second through holes for inserting EP tubes.

Preferably: and a plurality of third through holes which are in one-to-one correspondence with the plurality of second through holes are formed in the flat plate support.

Preferably: the plurality of second through holes include through holes of a plurality of different hole diameters.

Preferably: the thickness of the plate support is the same as that of the 384-well fluorescent quantitative PCR plate.

Preferably: be provided with first arc nest portion and second arc nest portion on the corresponding both sides wall of concave type accommodation portion respectively, first arc nest portion is located 384 hole fluorescence quantitative PCR plate's both sides, second arc nest portion is located plate holder's both sides.

According to the utility model provides a concrete embodiment, the utility model discloses a following technological effect:

through the utility model, a 384-hole fluorescence quantitative PCR plate ice box fixing device can be realized, and in an implementation mode, the device can comprise a multipurpose rectangular ice box, wherein a flat-layer full clapboard is formed at the inner side of the multipurpose rectangular ice box, and the upper part of the clapboard and the box wall positioned above the clapboard are enclosed together to form a concave accommodating part for accommodating a 384-hole fluorescence quantitative PCR plate; the length of the upper surface of the partition board is larger than that of the 384-hole fluorescent quantitative PCR plate; a plate holder for filling a gap in a longitudinal direction between the 384-well fluorogenic quantitative PCR plate and the multipurpose rectangular ice box so as to restrict a movement of the 384-well fluorogenic quantitative PCR plate in the longitudinal direction with respect to the partition. The application provides a 384 hole fluorescence ration PCR board ice box fixing device, multi-purpose rectangle ice box can laminate with 384 hole board bottom closely, has guaranteed low temperature and fixed effect, has promoted experimenter's operation accuracy and experimental speed to improve experimental efficiency. The plate support is also provided with a through hole with the aperture corresponding to that of the multipurpose rectangular ice box, so that the temporary storage reagent EP tube can be conveniently used.

Of course, it is not necessary for any particular product to achieve all of the above-described advantages at the same time.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings required to be used in the embodiments will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.

FIG. 1 is a schematic structural diagram of a 384-well fluorescence quantitative PCR plate ice box fixing device provided in an embodiment of the present invention;

fig. 2 is a cross-sectional view of a-a plane provided by an embodiment of the present invention.

In the figure: the multifunctional rectangular ice box 1, a first arc-shaped socket part 11, a second arc-shaped socket part 12, a clapboard 2, a first through hole part 21, a second through hole part 22, a flat plate bracket 3, a third through hole 31 and a 384-hole fluorescence quantitative PCR plate 4.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art all belong to the protection scope of the present invention.

Examples

Referring to fig. 1 and 2, a 384-well fluorescence quantitative PCR plate ice box fixing device according to an embodiment of the present invention is shown in fig. 1 and 2, and the device includes a multi-purpose rectangular ice box 1, wherein a partition plate 2 is formed on an inner side of the multi-purpose rectangular ice box 1, and an upper portion of the partition plate 2 and a box wall located above the partition plate enclose a concave accommodating portion for accommodating a 384-well fluorescence quantitative PCR plate 4; the length of the upper surface of the partition board 2 is longer than that of the 384-well fluorescent quantitative PCR plate 4.

A plate holder 3 for filling a gap in a long direction between the 384-well fluorogenic quantitative PCR plate 4 and the multipurpose rectangular ice case 1 so as to restrict a movement of the 384-well fluorogenic quantitative PCR plate 4 in the long direction with respect to the partition 2.

Specifically, one side surface of the plate holder 3 is configured to be in contact with the partition plate 2, and two vertical surfaces of the plate holder 3 in the width direction are respectively configured to be in contact with one vertical surface of the 384-well fluorescent quantitative PCR plate 4 in the longitudinal direction and one vertical surface of the multipurpose rectangular ice box 1 in the longitudinal direction. For better fixing effect, the length of the plate holder 3 is the same as the width of the inside of the concave receptacle, and the difference between the width of the plate holder 3 and the length of the inside of the concave receptacle and the length of the 384-well quantitative fluorescence PCR plate 4 is the same. The thickness of the plate holder is the same as that of the 384-well fluorescent quantitative PCR plate 4.

Further, a first through hole portion 21 and a second through hole portion 22 are formed on the partition plate 2, the first through hole portion 21 includes 384 first through holes corresponding to 384 wells of the 384-well fluorescent quantitative PCR plate 4, and the second through hole portion 22 includes a plurality of second through holes for inserting EP tubes. The flat plate support 3 is provided with a plurality of third through holes 31 corresponding to the plurality of second through holes one to one. This third through-hole can be used for keeping in EP pipe, in order to can keep in the EP pipe of multiple different diameters, a plurality of the through-hole of second through-hole including multiple different apertures. In order to facilitate taking out the 384-well fluorescence quantitative PCR plate and the plate support from the concave accommodating part, two side walls corresponding to the concave accommodating part are respectively provided with a first arc-shaped socket part 11 and a second arc-shaped socket part 12, the first arc-shaped socket part 11 is located at two sides of the 384-well fluorescence quantitative PCR plate 4, and the second arc-shaped socket part 12 is located at two sides of the plate support 3.

The device that this application provided can be fixed in 384 hole fluorescence quantitative PCR boards on multi-purpose rectangle ice chest through the plate holder, enables 384 hole fluorescence quantitative PCR boards and keeps the low temperature and just fix and can not take place to sideslip about on the ice chest in the experimentation, still has aperture and multi-purpose rectangle ice chest on the support aperture to correspond the through-hole, and the reagent EP pipe of being convenient for keep in is used. In the specific preparation, a 384-well fluorescent quantitative PCR plate with a length of 12.8cm was placed on a multipurpose rectangular ice box, and the inside diameter of the surface of the multipurpose ice box was 16cm in length and 9.2cm in width. Preparing a flat plate support with the length of 9.2cm and the width of 3.2cm, and forming holes on the flat plate at the same position as the aperture of the ice box base. Finally, the plate holder was placed on the left end of the PCR plate for immobilization. The aperture of the bracket is corresponding to the aperture of the multipurpose rectangular ice box, so that the reagent EP pipe can be temporarily stored conveniently.

In a word, 384 hole fluorescence quantitative PCR board ice box fixing device that this application provided, multi-purpose rectangle ice box can laminate closely with 384 hole board bottom, has guaranteed low temperature and fixed effect, has promoted experimenter's operation accuracy and experimental speed to improve experimental efficiency. The plate support is also provided with a through hole with the aperture corresponding to that of the multipurpose rectangular ice box, so that the temporary storage reagent EP tube can be conveniently used.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus. Without further limitation, an element defined by the phrase "comprising an … …" does not exclude the presence of other identical elements in a process, method, article, or apparatus that comprises the element.

The above description is only a preferred embodiment of the present invention, and is not intended to limit the scope of the present invention. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention shall fall within the protection scope of the present invention.

Claims (8)

1. A384-well fluorescence quantitative PCR plate ice box fixing device is characterized by comprising:

the multifunctional rectangular ice box is characterized in that a flat-layer full clapboard is formed on the inner side of the multifunctional rectangular ice box, and the upper part of the clapboard and the box wall positioned above the clapboard jointly enclose a concave accommodating part for placing a 384-hole fluorescent quantitative PCR plate; the length of the upper surface of the partition board is larger than that of the 384-hole fluorescent quantitative PCR plate;

a plate holder for filling a gap in a longitudinal direction between the 384-well fluorogenic quantitative PCR plate and the multipurpose rectangular ice box so as to restrict a movement of the 384-well fluorogenic quantitative PCR plate in the longitudinal direction with respect to the partition.

2. The 384-well fluorescence quantitative PCR plate ice box fixing device according to claim 1, wherein one side surface of the plate holder is used for abutting against the partition plate, and two vertical surfaces of the plate holder in the width direction are respectively used for abutting against one vertical surface of the 384-well fluorescence quantitative PCR plate in the length direction and one vertical surface of the multipurpose rectangular ice box in the length direction.

3. The 384-well fluorescence quantitative PCR plate ice cassette holder according to claim 2, wherein the length of the plate holder is the same as the width of the inside of the concave receiving part, and the difference between the width of the plate holder and the length of the inside of the concave receiving part is the same as the length of the 384-well fluorescence quantitative PCR plate.

4. The 384-well fluorescence quantitative PCR plate ice box fixing device according to claim 1, wherein the partition plate is formed with a first through-hole portion including 384 first through-holes corresponding to the 384 wells of the 384-well fluorescence quantitative PCR plate, and a second through-hole portion including a plurality of second through-holes for inserting EP tubes.

5. The 384-well fluorescence quantitative PCR plate ice box fixing device according to claim 4, wherein the plate holder is provided with a plurality of third through holes corresponding to the plurality of second through holes one to one.

6. The 384-well fluorescence quantitative PCR plate ice cassette holder device according to claim 4, wherein the plurality of second through-holes comprises a plurality of through-holes with different pore diameters.

7. The 384-well fluorescence quantitative PCR plate ice cassette holder of claim 1, wherein the thickness of the plate holder is the same as the thickness of the 384-well fluorescence quantitative PCR plate.

8. The 384-well fluorescence quantitative PCR plate ice box fixing device of claim 1, wherein two corresponding side walls of the concave receiving portion are respectively provided with a first arc-shaped socket portion and a second arc-shaped socket portion, the first arc-shaped socket portion is located at two sides of the 384-well fluorescence quantitative PCR plate, and the second arc-shaped socket portion is located at two sides of the plate holder.

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