CN214694150U - Epidermal cell separation and extraction instrument - Google Patents

Epidermal cell separation and extraction instrument Download PDF

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CN214694150U
CN214694150U CN202120583482.3U CN202120583482U CN214694150U CN 214694150 U CN214694150 U CN 214694150U CN 202120583482 U CN202120583482 U CN 202120583482U CN 214694150 U CN214694150 U CN 214694150U
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pool
cell separation
supporting structure
hole
incubation
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万绵水
林创鑫
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Abstract

The utility model provides an epidermic cell separation and extraction instrument, which comprises a shell, a supporting structure, an epidermic treatment operating panel and a filtering tank, wherein the shell comprises an accommodating tank with an upward opening, the supporting structure is arranged in the accommodating tank, the epidermic treatment operating panel is detachably arranged in the accommodating tank and at least partially supported by the supporting structure, the filtering tank is fixedly arranged on the supporting structure, the opening of the filtering tank is exposed from the supporting structure, and the bottom of the filtering tank is in a cone shape or a frustum shape; the epidermis treatment operation tray comprises a tray bottom and a tray wall, wherein the tray wall is enclosed along the periphery of the tray bottom and is used for placing skin tissues and buffer liquid and treating the skin tissues to obtain mixed liquid containing separated epidermal cells, and the filter tank is used for filtering the mixed liquid to obtain suspension containing the epidermal cells. The utility model discloses can fully collect cell suspension, not produce extravagantly.

Description

Epidermal cell separation and extraction instrument
Technical Field
The utility model relates to the technical field of medical treatment equipment, concretely relates to epidermic cell separation and extraction appearance.
Background
After a cell separation treatment using skin tissue separated from a human body, a cell suspension obtained for medical treatment (e.g., burn wound treatment) has become an important clinical treatment means. When the cell separation treatment is carried out, small pieces of skin tissue are firstly cut from organisms such as human bodies, and then the suspension containing the successfully separated epidermal cells is obtained through seven working steps of incubation, cleaning, scraping treatment, mixing, primary collection, filtration and secondary collection.
Specifically, "incubation" is a digestive treatment of skin tissue excised from a living body with an appropriate type of digestive enzyme for an appropriate time; "washing" is to wash away the digestive enzymes on the skin surface with a buffer after the incubation step is finished; the scraping treatment is moderate scraping treatment of the cleaned skin tissue; the step of mixing is to add a buffer solution into the raw materials obtained after the scraping treatment step to obtain a mixed solution: the primary collection is to collect the mixed solution by using a syringe; the filtering step is to put the mixed liquid obtained after the primary collection into a filtering tank with a filter screen to filter and remove impurities; the secondary collection is to collect the cell suspension retained in the filter tank by using a syringe for medical treatment. Typically about 1 ml of cell suspension is prepared for a 1 cm square of human skin, and about 80 cm square of cell suspension is used to treat a treatment area.
SUMMERY OF THE UTILITY MODEL
Based on the above current situation, the main object of the present invention is to provide an epidermal cell separation and extraction instrument, which can fully collect cell suspension without waste.
In order to achieve the above object, the utility model adopts the following technical scheme:
an epidermal cell separation and extraction instrument comprises a shell, a supporting structure, an epidermal treatment operation disc and a filter tank, wherein the shell comprises a containing groove with an upward opening, the supporting structure is arranged in the containing groove, the epidermal treatment operation disc is detachably arranged in the containing groove and is at least partially supported by the supporting structure, the filter tank is fixedly arranged on the supporting structure, the opening of the filter tank is exposed from the supporting structure, and the bottom of the filter tank is in a shape of a cone or a frustum; the epidermis treatment operation tray comprises a tray bottom and a tray wall, wherein the tray wall is enclosed along the periphery of the tray bottom and is used for placing skin tissues and buffer liquid and treating the skin tissues to obtain mixed liquid containing separated epidermal cells, and the filter tank is used for filtering the mixed liquid to obtain suspension containing the epidermal cells.
Optionally, the epidermic cell separation and extraction instrument comprises a cover body, and the cover body can be pivoted to cover the shell body.
Optionally, the epidermic cell separation and extraction instrument further comprises a buffer solution containing pool, the buffer solution containing pool is fixedly mounted to the supporting structure, an opening of the buffer solution containing pool is exposed from the supporting structure, and the bottom of the buffer solution containing pool is in a shape of a vertebral body or a vertebral platform.
Optionally, the epidermic cell separation and extraction instrument further comprises an incubation pool fixedly mounted to the support structure, and a pool opening of the incubation pool is exposed from the support structure, wherein the pool opening area of the incubation pool is smaller than the pool bottom area of the incubation pool.
Optionally, the support structure comprises a first platform comprising a first through hole, the mouth of the incubation well partially passes through the first through hole, the edge of the mouth of the incubation well is higher than the first platform, the mouth of the incubation well is provided with an outward turned edge, the end of the outward turned edge is lapped on the upper surface of the first platform, and a gap is arranged between the wall of the incubation well and the inner diameter of the first through hole.
Optionally, the first platform further comprises a second through hole and a third through hole; the buffer solution containing pool penetrates into the second through hole, and the opening of the buffer solution containing pool is flush with or higher than the second through hole; the filtering tank penetrates into the third through hole, and the opening of the filtering tank is parallel and level or higher than the third through hole.
Optionally, the epidermal cell separation and extraction instrument further comprises a display and control device, and the display and control device is fixed on the first platform and located beside the first through hole.
Optionally, the display and control device includes a display screen and keys disposed on the periphery of the display screen, where the keys include a time setting key.
Optionally, the tray wall of the skin treatment operation tray is provided with a recess at a position close to the side wall of the housing for taking out the skin treatment operation tray.
The utility model discloses an epidermic cell separation extractor, through setting up epidermis processing operation panel into detachable form, and make it include the dish wall and set the end, like this, accomplish in epidermis processing operation panel after the moderate degree scraping to skin tissue is handled, add the buffer solution and obtain mixed liquid and collect the later stage to the buffer solution, can take off epidermis processing operation panel from epidermic cell separation extractor, with its moderate degree slope, make the remaining mixed liquid in the operation panel gather in the local region in epidermis processing operation panel, the liquid level height has obviously been promoted, the syringe that is convenient for be used for absorbing and collects mixed liquid can fully collect remaining mixed liquid from this local region; the mixed liquid is fully collected and then is put into a filter tank, and the injector which is convenient for collecting the cell suspension liquid fully collects the residual cell suspension liquid from the bottom of the filter tank when the cell suspension liquid is collected to the later stage through the design of a cone body or a frustum at the bottom of the filter tank. The utility model provides the high collection rate of cell suspension, with the living biological skin of same area, can collect more cell suspensions for the treatment area of bigger area.
Other advantages of the present invention will be described in the detailed description, and those skilled in the art can understand the technical advantages brought by the technical features and technical solutions through the descriptions of the technical features and the technical solutions.
Drawings
Preferred embodiments of the present invention will be described below with reference to the accompanying drawings. In the figure:
fig. 1 is a schematic perspective view of an preferred embodiment of an epidermal cell separation and extraction instrument provided by the present invention;
fig. 2 is a schematic diagram of an explosion structure of a preferred embodiment of the epidermal cell separation and extraction instrument provided by the present invention;
FIG. 3 is a schematic rear view of the preferred embodiment of the epidermic cell separation and extraction instrument provided by the present invention with the cover and the housing removed;
fig. 4 is a schematic top view of a preferred embodiment of the first platform of the epidermal cell separation and extraction instrument provided by the present invention;
fig. 5 is a schematic perspective view of an embodiment of an operation panel for epidermis treatment of an apparatus for separating and extracting epidermal cells according to the present invention.
Detailed Description
The present invention will be described below based on examples, but the present invention is not limited to only these examples. In the following detailed description of the present invention, certain specific details are set forth in order to avoid obscuring the spirit of the present invention, well-known methods, procedures, flows, and components have not been described in detail.
Further, those of ordinary skill in the art will appreciate that the drawings provided herein are for illustrative purposes and are not necessarily drawn to scale.
Unless the context clearly requires otherwise, throughout the description and the claims, the words "comprise", "comprising", and the like are to be construed in an inclusive sense as opposed to an exclusive or exhaustive sense; that is, what is meant is "including, but not limited to".
In the description of the present invention, it is to be understood that the terms "first," "second," and the like are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. In addition, in the description of the present invention, "a plurality" means two or more unless otherwise specified.
Referring to the description of the drawings 1-2, the present invention provides an epidermal cell separation and extraction instrument, including a housing 400, a supporting structure 300, an epidermal treatment operating disk 200 and a filtering basin 500, wherein the housing includes a receiving tank 410 with an upward opening, the supporting structure 300 is disposed in the receiving tank 410, the epidermal treatment operating disk 200 is detachably disposed in the receiving tank and is at least partially supported by the supporting structure 300, the filtering basin 500 is fixedly mounted to the supporting structure 300, and the opening of the filtering basin 500 is exposed from the supporting structure 300, and the bottom of the filtering basin is in the shape of a cone or a frustum; the epidermis treatment operation tray 200 comprises a tray bottom 210 and a tray wall 220, wherein the tray wall 220 is enclosed along the periphery of the tray bottom 210 and is used for placing skin tissues and buffer liquid and treating the skin tissues to obtain mixed liquid containing separated epidermis cells, and the filter tank 500 is used for filtering the mixed liquid to obtain suspension containing the epidermis cells.
Specifically, the utility model provides an epidermic cell separation extraction apparatus, with supporting structure 300 with epidermis processing operation panel 200 and filtering ponds 500 integration in the casing 400 of extraction apparatus, the user utilize inside this extraction apparatus alright accomplish the scraping to skin tissue and handle so as to obtain the mixed liquid that contains the epidermis cell of separation and filter the work step of mixed liquid so as to obtain the suspension that contains the epidermis cell. Compare in handling operating element and filter element with epidermis and set up mutually independently completely, the utility model discloses be favorable to drawing the integrated setting of the component in the work with epidermal cell separation, be convenient for accomodate, also be difficult for losing.
Further, the utility model discloses a set up epidermis treatment operation panel 200 into detachable form, and make it include wall of a plate 220 and end of a plate 210, thus, accomplish in epidermis treatment operation panel 200 after the scraping of skin tissue is handled, add the buffer solution and obtain mixed liquid and once collect the later stage to the buffer solution, because this moment in the dish mixed liquid remain not much, the liquid level is lower, be unfavorable for the syringe to the collection of remaining mixed liquid, alright take off epidermis treatment operation panel 200 from epidermis cell separation extraction appearance this moment, with its moderate degree slope, make remaining mixed liquid gather in operation panel 200 in the inside lower region of epidermis treatment operation panel 200, the liquid level has obviously been promoted, be convenient for be used for absorbing the syringe of collecting mixed liquid and can fully collect remaining mixed liquid from this region.
Furthermore, the utility model discloses not only can collect mixed liquid fully for the mixture is dropped into filtering ponds 500 completely almost without waste, still through the centrum or the frustum design of filtering ponds 500 bottom, when can carrying out the secondary to cell suspension and collect the later stage, promotes the liquid level height of remaining cell suspension by the design of centrum or frustum, and the syringe of being convenient for collect cell suspension fully collects remaining cell suspension from filtering ponds 500 bottoms. The utility model provides the high collection rate of once collecting and secondary collection uses the living biological skin of same area, can collect more cell suspensions for the treatment area of bigger area.
As a preferred embodiment, referring to FIG. 2 of the specification, the epidermis treating operation panel 200 is integrally and detachably mounted in the holding groove of the support structure 300, and the support structure is mounted in the holding groove of the epidermis cell extracting apparatus, thereby achieving that the epidermis treating operation panel 200 is indirectly and detachably mounted in the holding groove. Of course, other forms of support structures may be employed such that the epidermal treatment manipulating disk 200 is directly seated in the receiving groove of the epidermal cell extraction instrument.
It will be understood by those skilled in the art that the openings of the filtering tank 500, and the buffer solution holding tank 600 and the incubation tank 700 mentioned below, may be formed in various shapes, such as a circle, a square, etc. Meanwhile, the above-mentioned support structure 300 may be an integrated support structure that supports both the filtration tank and the skin treatment operation panel; or two different supporting structures which are arranged separately can be used for respectively supporting the filtering tank and the skin treatment operating panel.
Optionally, referring to fig. 1-2 of the specification, the epidermic cell separation and extraction instrument comprises a cover body 100, and the cover body 100 can be pivoted to cover the shell 400.
By providing the cover body 100 to the epidermic cell separation and extraction instrument, various elements inside are protected favorably, so that the epidermic cell separation and extraction instrument is not easy to be dusted or fall off from the extraction instrument. Further, when the epidermic cell separation and extraction instrument includes the incubation pool 700 inside, because incubation is often required to be performed under a heating condition, the cover 100 completely covers the casing 400 of the epidermic cell separation and extraction instrument, so that heat emitted from the opening of the incubation pool 700 can be prevented from being diffused to the outside, heat loss of the incubation pool 700 is reduced, and a heat preservation effect of the incubation pool is improved.
Optionally, referring to the description of fig. 2-3, the epidermic cell separation and extraction instrument further comprises a buffer solution containing pool 600, the buffer solution containing pool 600 is fixedly mounted to the supporting structure 300, an opening of the buffer solution containing pool 600 is exposed from the supporting structure 300, and the bottom of the buffer solution containing pool 600 is in a shape of a vertebral body or a vertebral platform.
The utility model discloses still set up buffer solution and hold pond 600 in epidermal cell separation extraction appearance, can just add buffer solution with buffer solution and hold in the pond 600 when carrying out epidermal cell separation work and beginning. Thus, after the incubation step is completed, the incubated skin tissue can be directly put into the buffer solution containing pool 600 for cleaning, and then the skin tissue is clamped out from the buffer solution containing pool 600 and put into the epidermis treatment operation tray 200 for scraping treatment. After the scraping treatment step is completed, the buffer solution in the buffer solution containing pool 600 (i.e. the buffer solution participating in the previous washing step) can be transferred to the epidermis treatment operation tray 200 by the injector to be mixed with the skin tissue, because the buffer solution containing pool 600 is also designed as a cone or a frustum, the buffer solution in the buffer solution containing pool 600 can be fully collected and utilized as much as possible, and the working step of mixing by adding the buffer solution from the outside again is avoided or reduced.
Optionally, referring to the description of fig. 2-3, the epidermic cell separation and extraction instrument further comprises an incubation pool 700, the incubation pool 700 is fixedly mounted to the support structure 300, and a pool opening 710 of the incubation pool 700 is exposed from the support structure, wherein the area of the pool opening 710 of the incubation pool is smaller than the area of the pool bottom of the incubation pool.
The utility model discloses a to hatching pond 700 and also set up to epidermis cell separation extraction appearance, further improved the component integration degree in the epidermis cell separation extraction work. Since the incubation step is often performed under a heating condition, in an alternative embodiment, the heating element is disposed at the bottom and/or the side of the incubation pool, and at this time, by setting the area of the pool opening 710 of the incubation pool to be smaller than the area of the pool bottom of the incubation pool, the heat dissipation from the pool opening 710 of the incubation pool can be significantly reduced, and the heat preservation effect of the incubation pool can be effectively improved.
Optionally, referring to the description of fig. 3-4, the support structure comprises a first platform 310, the first platform 310 comprises a first through hole 320, the mouth part of the incubation pool 700 passes through the first through hole 320, the edge of the mouth 710 of the incubation pool is higher than the first platform 310, the mouth 710 of the incubation pool is provided with an outward-turning bead, the end of the outward-turning bead is lapped on the upper surface of the first platform 310, and a gap is formed between the wall of the incubation pool 700 and the inner diameter of the first through hole 320.
Specifically, by providing the well port 710 of the incubation well 700 through the first through hole 320 of the first platform 310 in the support structure 300, the opening of the incubation well 700 is facilitated to be uncovered from the support structure; whereas a secure mounting of the incubation well to the support structure may be conveniently achieved by snapping the everted curled end of the well mouth 710 of the incubation well onto the upper surface of the first platform. Further, the provision of the out-turned bead may further slow the loss of heat from the mouth 710 of the incubation well. Moreover, a gap is left between the wall of the incubation pool 700 and the inner diameter of the first through hole 320, so that the situation that the heat inside the incubation pool is conducted out to the first platform 300 which is in close contact with the wall of the incubation pool 700 by the wall of the incubation pool 700 can be avoided, and the heat preservation effect of the incubation pool 700 is further improved.
Optionally, referring to fig. 3-4 of the specification, the first platform 310 further includes a second through hole 330 and a third through hole 340; the buffer solution containing pool 600 penetrates into the second through hole 330, and the mouth of the buffer solution containing pool 600 is flush with or higher than the second through hole 330; the filter tank 500 penetrates into the third through hole 340, and the tank opening of the filter tank 500 is flush with or higher than the third through hole 340.
Specifically, by making the buffer solution containing tank 600 and the filtering tank 500 respectively penetrate into the second through hole 330 and the third through hole 340 of the first platform 310, and making the tank openings of the tanks flush with or higher than the corresponding through holes, the tank openings of the buffer solution containing tank 600 and the filtering tank 500 are conveniently exposed from the supporting structure.
Optionally, referring to fig. 4 of the specification, the epidermic cell separation and extraction instrument further comprises a display and control device 800, wherein the display and control device 800 is fixed on the first platform 310 and is located beside the first through hole 320.
The utility model discloses a set up demonstration and controlling means 800, can promote the automation and the operation convenience of this epidermic cell separation extraction appearance greatly. Through this demonstration and controlling means 800, can realize for example that machine self-checking and fault suggestion, hatching pond heating condition set up and function such as heating state suggestion, hatching pond heating time control are showing the work efficiency who promotes the cell separation and draw, also can obviously promote user's experience degree.
Optionally, referring to fig. 4 in the specification, the display and control apparatus 800 includes a display 810 and keys 820 disposed on the periphery of the display 810, wherein the keys 820 include a time setting key.
Specifically, through the setting of the display screen 810, working parameters such as the heating temperature, the heating time and the like of the incubation pool can be displayed, so that a user can master the working state of the epidermal cell separation and extraction instrument in real time; through the setting of the time setting key, the heating time constant and other parameters of the incubation pool can be set as required, so that the heating time constant control is realized automatically.
Optionally, referring to fig. 5 of the specification, the tray wall 200 of the skin treatment operating tray 200 is provided with a recess 230 at a position close to the side wall of the housing for taking out the skin treatment operating tray 200.
Because the epidermis treatment operation panel needs to be taken down from the epidermis cell separation and extraction instrument in one collection process, the concave part is arranged at the part of the wall of the operation panel close to the side wall of the shell, a user can insert fingers into the concave part to apply force upwards to the concave part, and thus, the epidermis treatment operation panel can be taken down quickly and conveniently.
It will be appreciated by those skilled in the art that the above-described preferred embodiments may be freely combined, superimposed, without conflict.
It will be understood that the above-described embodiments are illustrative only and not restrictive, and that various obvious and equivalent modifications and substitutions may be made in the details described herein by those skilled in the art without departing from the basic principles of the invention.

Claims (9)

1. An epidermal cell separation and extraction instrument is characterized by comprising a shell (400), a supporting structure (300), an epidermal treatment operating disk (200) and a filter tank (500), wherein the shell (400) comprises a containing groove (410) with an upward opening, the supporting structure (300) is arranged in the containing groove (410), the epidermal treatment operating disk (200) is detachably arranged in the containing groove and is at least partially supported by the supporting structure (300), the filter tank (500) is fixedly arranged on the supporting structure (300), the opening of the filter tank (500) is exposed from the supporting structure (300), and the bottom of the filter tank (500) is in a cone or frustum shape; the epidermis treatment operation tray (200) comprises a tray bottom (210) and a tray wall (220), wherein the tray wall (220) is enclosed along the periphery of the tray bottom (210) and is used for placing skin tissues and buffer liquid and treating the skin tissues to obtain a mixed liquid containing separated epidermal cells, and the filter tank (500) is used for filtering the mixed liquid to obtain a suspension containing the epidermal cells.
2. The epidermic cell separation and extraction instrument according to claim 1, comprising a cover body (100), wherein the cover body (100) can pivotally cover the shell body (400).
3. The epidermic cell separation and extraction instrument according to claim 1, further comprising a buffer solution containing pool (600), wherein the buffer solution containing pool (600) is fixedly mounted to the supporting structure (300), an opening of the buffer solution containing pool (600) is exposed from the supporting structure (300), and a bottom of the buffer solution containing pool (600) is in a shape of a cone or a frustum of a cone.
4. The epidermic cell separation and extraction instrument according to claim 3, further comprising an incubation well (700), wherein the incubation well (700) is fixedly mounted to the support structure (300), and a well opening (710) of the incubation well (700) is exposed from the support structure (300), wherein the area of the well opening (710) of the incubation well (700) is smaller than the area of the bottom of the incubation well (700).
5. The epidermic cell separation and extraction instrument according to claim 4, wherein the support structure (300) comprises a first platform (310), the first platform (310) comprises a first through hole (320), the mouth (710) of the incubation pool (700) partially penetrates through the first through hole (320), the edge of the mouth (710) of the incubation pool (700) is higher than the first platform (310), the mouth (710) of the incubation pool (700) is provided with an outward-turned bead, the end of the outward-turned bead is lapped on the upper surface of the first platform (310), and a gap is formed between the wall of the incubation pool (700) and the inner diameter of the first through hole (320).
6. The epidermal cell separation and extraction instrument of claim 5, wherein said first platform (310) further comprises a second through hole (330) and a third through hole (340); the buffer solution containing pool (600) penetrates into the second through hole (330), and the opening of the buffer solution containing pool (600) is flush with or higher than the second through hole (330); the filtering tank (500) penetrates into the third through hole (340), and the opening of the filtering tank (500) is flush with or higher than the third through hole (340).
7. The epidermic cell separation and extraction instrument according to claim 5, further comprising a display and control device (800), wherein the display and control device (800) is fixed on the first platform (310) and is located beside the first through hole (320).
8. The epidermic cell separation and extraction instrument according to claim 7, wherein the display and control device (800) comprises a display screen (810) and a key (820) arranged on the periphery of the display screen, wherein the key (820) comprises a time setting key.
9. The epidermic cell separation and extraction instrument according to any one of claims 1-8, wherein the tray wall (220) of the epidermic treatment operating tray (200) is provided with a recess (230) at a position close to the side wall of the shell for taking the epidermic treatment operating tray.
CN202120583482.3U 2021-03-22 2021-03-22 Epidermal cell separation and extraction instrument Active CN214694150U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202120583482.3U CN214694150U (en) 2021-03-22 2021-03-22 Epidermal cell separation and extraction instrument

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202120583482.3U CN214694150U (en) 2021-03-22 2021-03-22 Epidermal cell separation and extraction instrument

Publications (1)

Publication Number Publication Date
CN214694150U true CN214694150U (en) 2021-11-12

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Application Number Title Priority Date Filing Date
CN202120583482.3U Active CN214694150U (en) 2021-03-22 2021-03-22 Epidermal cell separation and extraction instrument

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CN (1) CN214694150U (en)

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