CN214668024U - Microfluidic sample pretreatment chip - Google Patents
Microfluidic sample pretreatment chip Download PDFInfo
- Publication number
- CN214668024U CN214668024U CN202120124253.5U CN202120124253U CN214668024U CN 214668024 U CN214668024 U CN 214668024U CN 202120124253 U CN202120124253 U CN 202120124253U CN 214668024 U CN214668024 U CN 214668024U
- Authority
- CN
- China
- Prior art keywords
- module
- enrichment
- channel
- micro
- filtering
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 238000001914 filtration Methods 0.000 claims abstract description 62
- 238000002156 mixing Methods 0.000 claims abstract description 30
- 238000003491 array Methods 0.000 claims abstract description 7
- 239000002245 particle Substances 0.000 claims abstract description 7
- 239000000758 substrate Substances 0.000 claims description 24
- 238000002414 normal-phase solid-phase extraction Methods 0.000 claims description 16
- 239000002904 solvent Substances 0.000 claims description 12
- 238000006073 displacement reaction Methods 0.000 claims description 9
- 239000004205 dimethyl polysiloxane Substances 0.000 claims description 6
- 239000011521 glass Substances 0.000 claims description 6
- 229920000435 poly(dimethylsiloxane) Polymers 0.000 claims description 6
- 230000008676 import Effects 0.000 claims description 4
- 239000004033 plastic Substances 0.000 claims description 2
- -1 polydimethylsiloxane Polymers 0.000 claims description 2
- 238000005070 sampling Methods 0.000 claims description 2
- 238000002203 pretreatment Methods 0.000 claims 1
- 239000013049 sediment Substances 0.000 abstract description 8
- 235000013305 food Nutrition 0.000 abstract description 6
- 239000002244 precipitate Substances 0.000 abstract description 6
- 238000007781 pre-processing Methods 0.000 abstract description 3
- 238000000605 extraction Methods 0.000 abstract 1
- 238000002347 injection Methods 0.000 description 10
- 239000007924 injection Substances 0.000 description 10
- 238000000034 method Methods 0.000 description 8
- 235000013870 dimethyl polysiloxane Nutrition 0.000 description 5
- 238000001514 detection method Methods 0.000 description 4
- CXQXSVUQTKDNFP-UHFFFAOYSA-N octamethyltrisiloxane Chemical compound C[Si](C)(C)O[Si](C)(C)O[Si](C)(C)C CXQXSVUQTKDNFP-UHFFFAOYSA-N 0.000 description 4
- 238000004987 plasma desorption mass spectroscopy Methods 0.000 description 4
- 238000002493 microarray Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 2
- 238000005530 etching Methods 0.000 description 2
- 239000000706 filtrate Substances 0.000 description 2
- 239000012535 impurity Substances 0.000 description 2
- 239000008267 milk Substances 0.000 description 2
- 210000004080 milk Anatomy 0.000 description 2
- 235000013336 milk Nutrition 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 238000005374 membrane filtration Methods 0.000 description 1
- 238000009832 plasma treatment Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 239000000243 solution Substances 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
The utility model discloses a micro-fluidic sample preprocessing chip, this micro-fluidic sample preprocessing chip include advance kind module, mixing module, filter module, enrichment module and go out kind module. The micro-column arrays with different sizes in the filtering module can be used for fractional filtration of sample sediments, so that the channel is prevented from being blocked; the SPE unit in the enrichment module is filled with C18 particles, and the tail end of the enrichment channel is provided with a dam structure which can be used for target extraction and enrichment. Therefore, the utility model discloses have the function of sample precipitate stage filtration and target enrichment concurrently, can extensively be used for the sample preliminary treatment that food detected.
Description
Technical Field
The utility model relates to a micro-fluidic sample pretreatment chip belongs to food detection area.
Background
Currently, in the process of detecting drug or toxin residue in a food sample, the sample needs to be pretreated first. The pretreatment process of the food sample is complicated, comprises the processes of centrifugation, filter membrane filtration and the like of the sample, the pretreatment time is long, and a large amount of organic reagents are consumed. For example, when detecting antibiotic residues in milk, proteins in the milk need to be precipitated in advance, then the precipitate needs to be filtered, and the filtrate is purified and enriched by using an SPE (solid phase extraction) column, and finally the target object can be detected. The method is mature and high in recognition, but is long in time consumption and complicated in steps. Therefore, there is a need in the art to develop a method or apparatus that can be used for sample sediment filtration and that reduces the use of organic reagents, and that can simply and rapidly pre-treat a sample.
SUMMERY OF THE UTILITY MODEL
Utility model purpose: the utility model aims at providing a micro-fluidic preprocessing chip of sample precipitate filtration and target enrichment, micro-fluidic chip have the function of sample filtration and target enrichment concurrently, can effectively get rid of precipitate impurity in the sample in the food sample testing process, avoid impurity to follow-up detection reaction's influence to effectively prevented the passageway and blockked up, through enriching the target, further improved the accuracy and the reliability of testing result.
The technical scheme is as follows: the microfluidic sample pretreatment chip of the utility model is formed by bonding an upper substrate and a lower substrate, and comprises a sample introduction module, a mixing module, a filtering module, an enrichment module and a sample outlet module; the sampling module includes sample introduction port and solvent introduction port, the mixing module includes mixing channel, filtering module includes import, filtering channel and export, the enrichment module includes entry and enrichment passageway, the enrichment passageway contains the solid phase extraction unit, the appearance module is including a appearance mouth, the sample introduction port with the junction of solvent introduction port with mixing channel's front end communicates, mixing channel's rear end with filtering module's import is linked together, filtering module's export with the entry of enrichment module is linked together, the enrichment passageway is linked together with a appearance mouth of appearance module.
Further, the sample injection port, the solvent injection port, the outlet, the inlet and the sample outlet are arranged on the upper substrate, and the mixing channel, the filtering channel and the enrichment channel are arranged on the lower substrate.
Furthermore, the filtering channels comprise a plurality of groups of filtering sub-channels which are connected in series, and the micro-column arrays with different sizes from large to small are sequentially arranged in the plurality of groups of filtering sub-channels.
Further, the filtering channel comprises 3 groups of filtering sub-channels connected in series, and each group of filtering sub-channels comprises 2 filtering sub-channels; the micro-column array in the first group of 2 filtering subchannels consists of a plurality of circular micro-columns with the diameter of 400 microns, the interval between the adjacent circular micro-columns is 160 microns, the displacement of 160 microns exists between every two rows of circular micro-columns, the micro-column array in the middle group of 2 filtering subchannels consists of a plurality of circular micro-columns with the diameter of 300 microns, the interval between the adjacent circular micro-columns is 120 microns, the displacement of 120 microns exists between every two rows of circular micro-columns, the micro-column array in the later group of 2 filtering subchannels consists of a plurality of circular micro-columns with the diameter of 200 microns, the interval between the adjacent circular micro-columns is 120 microns, and the displacement of 80 microns exists between every two rows of circular micro-columns.
Further, the solid phase extraction unit is filled with C18 particles with the diameter of 30-60 μm.
Furthermore, a dam structure is designed at the outlet of the tail end of the enrichment channel, and the distance between the upper end of the dam structure and the upper layer substrate is 30 micrometers.
Furthermore, the inner wall of the mixing channel is etched with rectangular micro-columns with the length of 600 microns and the width of 200 microns, the included angle alpha between each rectangular micro-column and the inner wall of the mixing channel is 45 degrees, and the included angle beta is 135 degrees.
Furthermore, the upper substrate, the lower substrate, the circular microcolumns, the rectangular microcolumns and the dam structure are made of one or more of glass, polydimethylsiloxane or plastic.
Further, the circular microcolumns, the rectangular microcolumns, the dam structures, and the like are formed by etching the lower substrate.
Has the advantages that: compared with the prior art, the utility model has the advantages of it is as follows showing: according to the micro-fluidic pretreatment chip, a plurality of groups of filtering sub-channels are connected in series, and circular micro-columns with different diameters are designed in the micro-column array of the filtering channels, so that the sediment in a sample can be filtered in a grading manner, the sediment can be effectively removed, and the chip can be prevented from being blocked. And C18 particles with the diameter of 30-60 mu m are filled in the solid phase extraction unit in the enrichment module, and the tail end of the enrichment channel is designed with a dam structure, so that the target can be enriched, the accuracy and reliability of a detection result are improved, and the enrichment module can be widely used for pretreatment of samples in food detection.
Drawings
Fig. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic cross-sectional view of the first two filtration sub-channel microarrays within a filtration channel;
FIG. 3 is a schematic cross-sectional view of the middle two filter channel microarrays within a filter channel;
FIG. 4 is a schematic cross-sectional view of the last two filter channel microarrays within a filter channel;
FIG. 5 is a cross-sectional view of an enrichment channel SPE unit;
FIG. 6 is a close-up view of the mixing channel.
Detailed Description
The technical solution of the present invention will be further explained with reference to the accompanying drawings.
The microfluidic pretreatment chip of the present embodiment is designed to meet the requirements of filtration of sample precipitation and enrichment of trace target substances. Therefore, the utility model discloses designed not unidimensional circular little post array in the filtration passageway of micro-fluidic preliminary treatment chip device, carried out hierarchical progressively filtering to the precipitate to prevent that the precipitate in the sample from blockking up the chip, diameter 50 mu mC18 granule and the end dam structure that sets up of enrichment passageway are filled through Solid Phase Extraction (SPE) unit in the enrichment passageway simultaneously, can enrich the trace object in the sample. Specifically, the method comprises the following steps:
as shown in fig. 1, the microfluidic pretreatment chip of the present invention is divided into an upper layer and a lower layer, and includes an upper PDMS substrate and a lower glass substrate stacked from top to bottom, and specifically includes a sample injection module, a mixing module, a filtering module, an enrichment module and a sample outlet module, wherein the sample injection module includes a sample injection port 1 and a solvent injection port 2, the mixing module includes a mixing channel 6, the filtering module includes an inlet, a filtering channel 7 and an outlet 4, the enrichment module includes an inlet 3 and an enrichment channel 8, the enrichment channel 8 includes a Solid Phase Extraction (SPE) unit, and the sample outlet module includes a sample outlet 5. Wherein, sample introduction port 1, solvent introduction port 2, sample outlet 5, outlet 4 and inlet 3 set up in upper PDMS substrate, and inlet 3 and outlet 4 are linked together through the external connection pipe. The mixing channel 6, the filtering channel 7 and the enrichment channel 8 are arranged on the lower glass substrate. The distance between the upper parts of the mixing channel 6, the filtering channel 7 and the enrichment channel 8 and the upper-layer PDMS substrate is 150 μm, and the upper-layer PDMS substrate and the lower-layer glass substrate are in fit connection through positioning ports, and are bonded with each other by plasma treatment. The junction of the sample injection port 1 and the solvent injection port 2 is communicated with the front end of the mixing channel 6, a rectangular microcolumn with the length of 600 microns and the width of 200 microns is etched on the inner wall of the mixing channel 6, the included angle alpha between the rectangular microcolumn and the inner wall of the mixing channel 6 is 45 degrees, and the included angle beta is 135 degrees, as shown in FIG. 6; the rear end of the mixing channel 6 communicates with the inlet of the filter module. The filtering channel 7 comprises three groups of 6 filtering component channels which are connected in series, and circular micro-column arrays with different sizes are respectively arranged in the three groups of filtering component channels. As shown in fig. 2, the front group of 2 filter subchannel microcolumn arrays consists of a plurality of circular microcolumns with the diameter of 400 μm, the interval between adjacent circular microcolumns is 160 μm, and 160 μm displacement exists between each row of circular microcolumns; as shown in fig. 3, the middle group of 2 filter subchannel microcolumn arrays consists of a plurality of circular microcolumns with the diameter of 300 μm, the interval between adjacent circular microcolumns is 120 μm, and the displacement between each row of circular microcolumns is 120 μm; as shown in fig. 4, the latter group of 2 filter subchannel microcolumn arrays consists of a plurality of circular microcolumns with the diameter of 200 μm, the interval between adjacent circular microcolumns is 120 μm, and 80 μm displacement exists between each row of circular microcolumns; as shown in fig. 5, the Solid Phase Extraction (SPE) unit in the enrichment channel 8 is filled with C18 particles with a diameter of 50 μm, the end of the enrichment channel 8 is provided with a dam structure, and the distance between the upper end of the dam structure and the upper substrate is 30 μm; the enrichment channel 8 is communicated with the sample outlet 5. All the micro columns, the dam structures and the like are formed by etching the lower substrate, and all the micro columns and the dam structures are made of glass.
The sample and the solvent are respectively added through an injection port 1 and a solvent injection port 2 and mixed in a mixing channel 6 of the mixing module. The rectangular microcolumn in the mixing channel 6 allows the sample and the solvent to be mixed well. The mixed sample then enters the filtration channel 7 of the filtration module. During filtering, the sediment with larger size in the sample can be intercepted by the circular microcolumn with the diameter of 400 μm in the first 2 filtering channels, and the sediment with smaller size further flows into the middle 2 filtering channels, is further intercepted by the circular microcolumn with the diameter of 300 μm, then flows into the back 2 filtering channels, and is intercepted by the circular microcolumn with the diameter of 200 μm, so that the sediment is intercepted in sequence, the purpose of graded filtering is realized, and the blockage of the inside of the chip by the sediment is avoided. The sample flows out from the outlet 4 of the filtering channel 7, and the inlet 3 at the front end of the enrichment channel 8 is connected with the outlet 4 of the filtering channel 7 through a connecting pipe, so that the filtrate is introduced into the enrichment channel 8. Solid Phase Extraction (SPE) unit in the enrichment channel 8 is filled with 50 μm C18 particles with diameter, which can enrich trace amount of target in the sample, and the dam structure at the end of the enrichment channel 8 intercepts 50 μm C18 particles in the enrichment channel 8. The target object flows out from the sample outlet 5 of the sample outlet module after being enriched, and the whole process of stage filtration and enrichment is completed.
Claims (8)
1. The microfluidic sample pretreatment chip is characterized by being formed by bonding an upper substrate and a lower substrate, and comprising a sample introduction module, a mixing module, a filtering module, an enrichment module and a sample outlet module; the sampling module includes sample introduction port (1) and solvent introduction port (2), the mixing module includes mixing channel (6), the filtering module includes import, filtering channel (7) and export (4), the enrichment module includes entry (3) and enrichment passageway (8), enrichment passageway (8) contain the solid phase extraction unit, it includes out appearance mouth (5) to go out the appearance module, sample introduction port (1) with the department of converging of solvent introduction port (2) with the front end of mixing channel (6) communicates, the rear end of mixing channel (6) with the import of filtering module is linked together, filtering module's export (4) with entry (3) of enrichment module are linked together, enrichment passageway (8) are linked together with play appearance mouth (5) of appearance module.
2. The microfluidic sample pretreatment chip according to claim 1, wherein the sample inlet (1), the solvent inlet (2), the outlet (4), the inlet (3), and the sample outlet (5) are disposed on an upper substrate, and the mixing channel (6), the filtering channel (7), and the enrichment channel (8) are disposed on a lower substrate.
3. The microfluidic sample pretreatment chip according to claim 1, wherein the filtration channel (7) comprises a plurality of groups of filtration sub-channels connected in series, and the groups of filtration sub-channels are sequentially provided with micro-column arrays with different sizes from large to small.
4. The microfluidic sample pre-treatment chip according to claim 3, wherein the filtration channel (7) comprises 3 sets of filtration subchannels connected in series, each set comprising 2 filtration subchannels; the micro-column array in the first group of 2 filtering subchannels consists of a plurality of circular micro-columns with the diameter of 400 microns, the interval between the adjacent circular micro-columns is 160 microns, the displacement of 160 microns exists between every two rows of circular micro-columns, the micro-column array in the middle group of 2 filtering subchannels consists of a plurality of circular micro-columns with the diameter of 300 microns, the interval between the adjacent circular micro-columns is 120 microns, the displacement of 120 microns exists between every two rows of circular micro-columns, the micro-column array in the later group of 2 filtering subchannels consists of a plurality of circular micro-columns with the diameter of 200 microns, the interval between the adjacent circular micro-columns is 120 microns, and the displacement of 80 microns exists between every two rows of circular micro-columns.
5. The microfluidic sample pretreatment chip of claim 1, wherein the solid phase extraction unit is filled with C18 particles having a diameter of 30-60 μm.
6. The microfluidic sample pretreatment chip according to claim 1, wherein an outlet at the end of the enrichment channel (8) is designed with a dam structure, and the upper end of the dam structure is 30 μm away from the upper substrate.
7. The microfluidic sample pretreatment chip according to claim 1, wherein the inner wall of the mixing channel (6) is etched with rectangular microcolumns with a length of 600 μm and a width of 200 μm, and the included angle α between the rectangular microcolumns and the inner wall of the mixing channel (6) is 45 ° and the included angle β is 135 °.
8. The microfluidic sample pretreatment chip according to claim 1, wherein the upper substrate, the lower substrate, the circular microcolumns, the rectangular microcolumns and the dam structure are made of one or more of glass, polydimethylsiloxane and plastic.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120124253.5U CN214668024U (en) | 2021-01-15 | 2021-01-15 | Microfluidic sample pretreatment chip |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120124253.5U CN214668024U (en) | 2021-01-15 | 2021-01-15 | Microfluidic sample pretreatment chip |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214668024U true CN214668024U (en) | 2021-11-09 |
Family
ID=78516131
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120124253.5U Active CN214668024U (en) | 2021-01-15 | 2021-01-15 | Microfluidic sample pretreatment chip |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214668024U (en) |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114308153A (en) * | 2021-12-17 | 2022-04-12 | 华南农业大学 | Solid-phase extraction micro-fluidic chip for detecting nafil substances and system for detecting nafil substances |
| CN115155400A (en) * | 2022-06-20 | 2022-10-11 | 南京祥中生物科技有限公司 | Full-automatic magnetic dispersion solid phase extraction device |
| CN115155683A (en) * | 2022-07-05 | 2022-10-11 | 中科合肥智慧农业协同创新研究院 | Centrifugal micro-fluidic chip and preparation method thereof |
| WO2023115717A1 (en) * | 2021-12-22 | 2023-06-29 | 清华大学深圳国际研究生院 | Microfluidic chip, microfluidic chip-based detection system, and method for detecting bacteria |
-
2021
- 2021-01-15 CN CN202120124253.5U patent/CN214668024U/en active Active
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114308153A (en) * | 2021-12-17 | 2022-04-12 | 华南农业大学 | Solid-phase extraction micro-fluidic chip for detecting nafil substances and system for detecting nafil substances |
| WO2023115717A1 (en) * | 2021-12-22 | 2023-06-29 | 清华大学深圳国际研究生院 | Microfluidic chip, microfluidic chip-based detection system, and method for detecting bacteria |
| CN115155400A (en) * | 2022-06-20 | 2022-10-11 | 南京祥中生物科技有限公司 | Full-automatic magnetic dispersion solid phase extraction device |
| CN115155400B (en) * | 2022-06-20 | 2023-08-08 | 南京祥中生物科技有限公司 | Full-automatic magnetic dispersion solid phase extraction device |
| CN115155683A (en) * | 2022-07-05 | 2022-10-11 | 中科合肥智慧农业协同创新研究院 | Centrifugal micro-fluidic chip and preparation method thereof |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN214668024U (en) | Microfluidic sample pretreatment chip | |
| CN102360010B (en) | Integrated microfluidic chip for capture of cancer cells in whole blood | |
| CN110093247B (en) | Micro-fluidic chip for enriching and capturing target cells of different specifications and sizes | |
| CN107262171A (en) | A kind of blood separation and culture chip and blood separating mechanism | |
| CN210916029U (en) | Simple micro-fluidic chip for separating and detecting circulating tumor cells | |
| CN113237799A (en) | Blood detection micro-fluidic chip | |
| CN103940660A (en) | Multichannel membrane enrichment device | |
| CN208604119U (en) | Micro-fluidic chip for circulating tumor cell sorting | |
| CN113817577B (en) | Liquid drop micro-fluidic chip with automatic nucleic acid extraction and purification functions | |
| CN105723203A (en) | Magnetic separation | |
| CN107400623B (en) | Micro-fluidic chip for automatically capturing circulating tumor cells and automatic capturing method thereof | |
| CN203929645U (en) | Micro-fluidic surface-enhanced Raman test chip | |
| CN104646079A (en) | Centrifugal CD micro-fluidic chip for capillary electrophoresis and capillary gel electrophoresis device | |
| CN108444797A (en) | A kind of water sample preprocessing system | |
| US20220001381A1 (en) | Microfluidic chip | |
| CN209352877U (en) | The multistage micro flow control chip device of circulating tumor cell sorting, enrichment and detection | |
| CN109746060B (en) | Micro-droplet generation chip | |
| CN109746058B (en) | Micro-droplet detection chip | |
| CN105510191B (en) | Streaming particle detection method | |
| CN109746063B (en) | Micro-droplet detection system | |
| CN113341030B (en) | High-flux liquid chromatography-mass spectrometry system and separation and analysis method | |
| CN112779118A (en) | Circulating tumor cell forward separation system | |
| CN108246373A (en) | Centrifugal type microfludic chip | |
| CN205382177U (en) | High flux polycell micro -fluidic chip device of built -in cell blocker | |
| CN106018613B (en) | It can switch online and realize the concatenated solid-phase extraction device of multicolumn and its extracting process |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |