CN214636380U - Buffer solution preparation device for HPLC purification - Google Patents
Buffer solution preparation device for HPLC purification Download PDFInfo
- Publication number
- CN214636380U CN214636380U CN202120441012.3U CN202120441012U CN214636380U CN 214636380 U CN214636380 U CN 214636380U CN 202120441012 U CN202120441012 U CN 202120441012U CN 214636380 U CN214636380 U CN 214636380U
- Authority
- CN
- China
- Prior art keywords
- reaction
- plug
- reagent bottle
- hplc purification
- reaction bottle
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Physical Or Chemical Processes And Apparatus (AREA)
Abstract
The utility model discloses a buffer solution configuration device for HPLC purification, including reagent bottle, trachea and reaction flask, the reaction flask sets up in one side of reagent bottle, just the bottleneck of reagent bottle is provided with the sealing plug, the bottleneck of reaction flask is provided with the plug, trachea one end runs through the center of sealing plug and extends to the inside upper end of reagent bottle, and the trachea other end runs through the center of plug and extends to the inside bottom of reaction flask. The device is provided with a pressure regulating valve in the middle of the air pipe, so that the reaction air pressure can be accurately controlled; the sealing plug of the device is provided with the sealing rubber table and the sealing ring, so that raw materials in the reagent bottle are effectively prevented from leaking in the reaction process, and the reaction efficiency is improved; the device is provided with a magnetic stirrer which is matched with a stirrer to stir reaction liquid, so that the reaction speed is accelerated, and the production efficiency is improved; this device sets up the cooling bath and cools down to the reaction process to cooperate the thermometer, monitor reaction temperature, guarantee the reactant quality.
Description
Technical Field
The utility model belongs to the technical field of bioengineering equipment, specifically, relate to a buffer solution configuration device for HPLC purification.
Background
The chromatography is firstly applied to the separation of plant pigments, in 1906, Russian botanists used calcium carbonate as a stationary phase to fill a vertical glass tube, the extract of the plant pigments flowed into the stationary phase, and after the plant pigments were eluted by petroleum ether, the plant pigments were dispersed into a plurality of parallel color bands by one color band in a calcium carbonate column, and the chromatography is so named. The classical liquid chromatography starts with the transport of the mobile phase by means of a liquid head in a large diameter glass column at room temperature and pressure, which is low column efficiency and long duration. High Performance Liquid Chromatography (HPLC) is based on classical liquid chromatography, and is developed rapidly by introducing a gas chromatography theory in the later stage of 60 years, and is different from the classical liquid chromatography in that solid phase filler particles are small and uniform, the small particles have high column efficiency, a mobile phase is conveyed under high pressure drive, and separation work is completed within several hours or even tens of minutes, so the HPLC is also called as the high pressure liquid chromatography.
In developing the HPLC method, there may be some compounds, and in the development process, a circle of chromatographic column is replaced, and an acidic, medium-sized, and basic mobile phase is tried, but the target compound is still not retained in the chromatographic column, and usually, these compounds have a relatively high polarity and are easily ionized, such as a strong acidic, strong basic compound, or some organic salts, and at this time, the common mobile phase and chromatographic column system have no effect on such compounds, and we need to use an ion-pair reagent, which is formed by strong hydrophilic ions, and combines with the ions on the analyte to form molecules that can be retained on the column. The existing ion pair reagent preparation device has low preparation efficiency and can not meet the requirement of high-efficiency production.
SUMMERY OF THE UTILITY MODEL
For the low technical problem of HPLC purification buffer solution preparation inefficiency that exists among the solution prior art, the utility model provides a buffer solution configuration device for HPLC purification.
The purpose of the utility model can be realized by the following technical scheme:
the utility model provides a buffer solution configuration device for HPLC purification, includes reagent bottle, trachea and reaction flask, the reaction flask sets up in one side of reagent bottle, just the bottleneck of reagent bottle is provided with the sealing plug, the bottleneck of reaction flask is provided with the plug, trachea one end runs through the center of sealing plug and extends to the inside upper end of reagent bottle, and the trachea other end runs through the center of plug and extends to the inside bottom of reaction flask.
As a further aspect of the present invention: a cooling tank is arranged below the reaction bottle, and the reaction bottle is arranged inside the cooling tank.
As a further aspect of the present invention: a magnetic stirrer is arranged below the cooling tank, and a stirrer is arranged inside the reaction bottle.
As a further aspect of the present invention: the reaction bottle is internally provided with a thermometer, and the detection end of the thermometer penetrates through the rubber plug and extends to the bottom end inside the reaction bottle.
As a further aspect of the present invention: and the middle part of the air pipe is provided with a pressure regulating valve.
As a further aspect of the present invention: the sealing plug is T style of calligraphy structure, and the vertical end outside of sealing plug is provided with two rings of joint compound platforms, the mounting hole that runs through is seted up at the sealing plug center, and has seted up the mounting groove on the pore wall of mounting hole, the clamping has the sealing washer in the mounting groove.
The utility model has the advantages that:
the utility model provides a buffer solution configuration device for HPLC purification, which has simple structure, low manufacturing cost and convenient operation; the device is provided with a pressure regulating valve in the middle of the air pipe, so that the reaction air pressure can be accurately controlled; according to the device, two circles of sealing rubber tables are arranged on the outer side of the vertical end of the sealing plug, the wall of the mounting hole is provided with the mounting groove, and the sealing ring is clamped in the mounting groove, so that raw materials in a reagent bottle are effectively prevented from leaking in the reaction process, and the reaction efficiency is improved; the device is provided with a magnetic stirrer which is matched with a stirrer to stir reaction liquid, so that the reaction speed is accelerated, and the production efficiency is improved; this device sets up the cooling bath and cools down to the reaction process to cooperate the thermometer, monitor reaction temperature, guarantee the reactant quality.
Drawings
In order to more clearly illustrate the technical solutions of the embodiments of the present invention, the drawings used in the description of the embodiments will be briefly introduced below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and it is obvious for those skilled in the art that other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic structural diagram of a buffer solution preparing apparatus for HPLC purification according to the present invention;
fig. 2 is a schematic view of the sealing plug.
In the drawings, the components represented by the respective reference numerals are listed below:
1. a reagent bottle; 2. a sealing plug; 21. a sealant platform; 22. mounting holes; 23. mounting grooves; 24. a seal ring; 3. an air tube; 4. a pressure regulating valve; 5. a rubber plug; 6. a reaction bottle; 7. a thermometer; 8. a cooling tank; 9. a stirrer; 10. and (4) stirring.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments of the present invention, all other embodiments obtained by a person of ordinary skill in the art without creative efforts belong to the protection scope of the present invention.
Referring to fig. 1-2, a buffer solution preparing device for HPLC purification includes a reagent bottle 1, a gas tube 3 and a reaction bottle 6, wherein the reaction bottle 6 is disposed at one side of the reagent bottle 1, a sealing plug 2 is disposed at a mouth of the reagent bottle 1, a rubber plug 5 is disposed at the mouth of the reaction bottle 6, one end of the gas tube 3 penetrates through the center of the sealing plug 2 and extends to the upper end of the inside of the reagent bottle 1, and the other end of the gas tube 3 penetrates through the center of the rubber plug 5 and extends to the bottom end of the inside of the reaction bottle 6.
A cooling tank 8 is arranged below the reaction bottle 6, and the reaction bottle 6 is arranged inside the cooling tank 8.
A magnetic stirrer 9 is arranged below the cooling tank 8, and a stirrer 10 is arranged in the reaction bottle 6; the reaction liquid is stirred, the reaction speed is accelerated, and the production efficiency is improved.
A thermometer 7 is arranged in the reaction bottle 6, and the detection end of the thermometer 7 penetrates through the rubber plug 5 and extends to the bottom end in the reaction bottle 6; the reaction temperature is regulated and controlled by matching with the cooling tank 8, and the quality of the reaction product is ensured.
The middle part of the air pipe 3 is provided with a pressure regulating valve 4; the reaction pressure can be accurately controlled.
The sealing plug 2 is of a T-shaped structure, two circles of sealing rubber platforms 21 are arranged on the outer side of the vertical end of the sealing plug 2, a penetrating mounting hole 22 is formed in the center of the sealing plug 2, a mounting groove 23 is formed in the wall of the mounting hole 22, and a sealing ring 24 is clamped in the mounting groove 23; effectively prevent the reaction in-process, the raw materials in the reagent bottle 1 are revealed, promote reaction efficiency.
The working principle is as follows:
to triethylamine carbonate buffer solution preparation right the utility model discloses the theory of operation: adding a proper amount of deionized water and triethylamine into a reaction bottle 6, driving a stirrer 10 to stir in the reaction bottle 6 through a magnetic stirrer 9 to fully mix reaction liquid, adding dry ice into a reagent bottle 1, gasifying the dry ice at normal temperature and normal pressure, introducing gaseous carbon dioxide into the reaction liquid in the reaction bottle 6 through an air pipe 3 to carry out chemical reaction, adding ice water into a cooling tank 8, controlling the reaction temperature through a thermometer 7, controlling the air inlet flow through a pressure regulating valve 4, completing the reaction, taking down a rubber plug 5, and regulating the pH of a product to complete the preparation of a buffer solution.
The foregoing is merely exemplary and illustrative of the structure of the invention and various modifications, additions or substitutions as are known to those skilled in the art may be made to the described embodiments without departing from the scope of the invention as defined in the accompanying claims.
Claims (6)
1. A buffer configuration device for HPLC purification, comprising: including reagent bottle (1), trachea (3) and reaction bottle (6), reaction bottle (6) set up in one side of reagent bottle (1), just the bottleneck of reagent bottle (1) is provided with sealing plug (2), the bottleneck of reaction bottle (6) is provided with plug (5), trachea (3) one end runs through the center of sealing plug (2) and extends to the inside upper end of reagent bottle (1), and trachea (3) other end runs through the center of plug (5) and extends to the inside bottom of reaction bottle (6).
2. A buffer configuration apparatus for HPLC purification according to claim 1, characterized in that: a cooling tank (8) is arranged below the reaction bottle (6), and the reaction bottle (6) is arranged inside the cooling tank (8).
3. A buffer configuration apparatus for HPLC purification according to claim 2, characterized in that: a magnetic stirrer (9) is arranged below the cooling tank (8), and a stirrer (10) is arranged inside the reaction bottle (6).
4. A buffer configuration apparatus for HPLC purification according to claim 1, characterized in that: the reaction bottle (6) is internally provided with a thermometer (7), and the detection end of the thermometer (7) penetrates through the rubber plug (5) and extends to the bottom end inside the reaction bottle (6).
5. A buffer configuration apparatus for HPLC purification according to claim 1, characterized in that: and the middle part of the air pipe (3) is provided with a pressure regulating valve (4).
6. A buffer configuration apparatus for HPLC purification according to claim 1, characterized in that: sealing plug (2) are T style of calligraphy structure, and the vertical end outside of sealing plug (2) is provided with two rings of joint compound platform (21), mounting hole (22) that run through have been seted up at sealing plug (2) center, and have seted up mounting groove (23) on the pore wall of mounting hole (22), sealing washer (24) have been equipped with to the card in mounting groove (23).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120441012.3U CN214636380U (en) | 2021-03-01 | 2021-03-01 | Buffer solution preparation device for HPLC purification |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120441012.3U CN214636380U (en) | 2021-03-01 | 2021-03-01 | Buffer solution preparation device for HPLC purification |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214636380U true CN214636380U (en) | 2021-11-09 |
Family
ID=78450030
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120441012.3U Active CN214636380U (en) | 2021-03-01 | 2021-03-01 | Buffer solution preparation device for HPLC purification |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214636380U (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114570295A (en) * | 2022-03-25 | 2022-06-03 | 上海吉量医药工程有限公司 | Method for rapidly preparing triethylamine carbonate buffer solution |
-
2021
- 2021-03-01 CN CN202120441012.3U patent/CN214636380U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114570295A (en) * | 2022-03-25 | 2022-06-03 | 上海吉量医药工程有限公司 | Method for rapidly preparing triethylamine carbonate buffer solution |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN214636380U (en) | Buffer solution preparation device for HPLC purification | |
| CN103922980B (en) | A kind of method utilizing bipolar membrane electrodialysis to isolate methionine from the mixed solution of methionine salt and carbonate | |
| CN205667835U (en) | A kind of visualization stirring-type gas hydration reaction experimental provision | |
| CN203916532U (en) | A kind of Double stirring device that is applicable to small paint scuffing of cylinder bore | |
| CN113358810A (en) | Device and method for generating microbubbles and generating hydrate in situ by using visual kettle | |
| CN109776424B (en) | Novel method for preparing L-phenylglycine precursor phenylhydantoin by using MIC reactor | |
| CN105036171A (en) | Novel technology for continuously producing light calcium carbonate | |
| CN104370783B (en) | Hydrogen sulfide recycling method and hydrogen sulfide recycling system for continuously producing mercaptoacetic acid by adopting sodium hydrosulfide method | |
| CN111892224B (en) | Ammonia-nitrogen wastewater treatment system and method based on south crossing dialysis and osmotic distillation coupling | |
| DK287887D0 (en) | PROCEDURE AND CONTAINER FOR THE PREPARATION OF POLYSACCARIDES, NAME XANTHAN | |
| CN106006776B (en) | A kind of hydrate sewage water treatment method and device | |
| CN207684974U (en) | New membrane is aerated membrane bioreactor | |
| CN109231572A (en) | A kind of processing system and technique of ammonia nitrogen waste water | |
| CN109455753A (en) | Etching solution recycles technique | |
| CN104229838A (en) | Method for improving carbonization efficiency of lithium carbonate by controlling gas flow | |
| Bauer et al. | Mass transfer characteristics of Venturi liquid-gas contactor | |
| CN203295267U (en) | Carbon dioxide dissolving reaction device | |
| CN209428509U (en) | A kind of aerobic-anaerobic compound bacteria fermentation purifying tank | |
| CN112960813A (en) | Method for improving CO extracted from seawater2Device and method for acidifying seawater with purity | |
| CN205413009U (en) | Gas -liquid reaction device | |
| JPS6027379A (en) | Biochemical reactor | |
| CN215415259U (en) | Use visual cauldron normal position to produce microbubble and generate experimental apparatus of hydrate | |
| CN107298644A (en) | The method that bipolar membrane electrodialysis device prepares TMAH | |
| CN104150456B (en) | The method producing synthos and sodium ascorbyl phosphate with sodium sulfate wastewater | |
| SU955976A1 (en) | Device for removing gas from liquid |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CP01 | Change in the name or title of a patent holder | ||
| CP01 | Change in the name or title of a patent holder |
Address after: 239000 No.69, qifushi West Road, Chuzhou economic and Technological Development Zone, Anhui Province Patentee after: General Biology (Anhui) Co.,Ltd. Address before: 239000 No.69, qifushi West Road, Chuzhou economic and Technological Development Zone, Anhui Province Patentee before: GENERAL BIOSYSTEMS (ANHUI), Inc. |