CN214621913U - 13CO2 mark cultivation-root secretion collection device shared by water and soil environments - Google Patents

Abstract

The utility model discloses a soil and water environment sharing¹³CO₂The device for collecting the secretion of root system for marker culture includes an upper isotope gas marker box and a lower plant root box, the marker box includes a glass cover, an air mixing device and a gas valve¹³CO₂A gas cylinder, a plant root box is divided into a left solution culture chamber and a right soil culture chamber from left to right by a PVC plastic plate, the soil is divided into a plurality of longitudinal interlayers by a nylon net in the right soil culture chamber,a Micro Rhizon rhizosphere solution sampler is embedded in each separated root system growth chamber. The utility model provides a plant shared by water and soil environment¹³CO₂The device for collecting the root exudates has the characteristics of simple structure, small occupied area and low cost, can separate the organic matters of the soil from the root exudates on the premise of not damaging the growth of the root system, and can accurately position, collect and analyze the distribution characteristics of the root exudates in different rhizosphere micro-areas.

Description

Shared by water and soil environment13CO2Marker culture-root secretion collecting device

Technical Field

The utility model relates to a root system secretion collects technical field, in particular to plant of soil and water environment sharing¹³CO₂Marker culture-root exudate collection integrated device.

Background

Plants can alter the soil microflora by secreting biologically active molecules into the rhizosphere, thereby affecting the performance of their progeny. Root exudates, in addition to providing carbon and nitrogen substrates for microbial growth, affect rhizosphere microorganisms as signal molecules, attractants, stimulants, inhibitors, and repellents. The root exudates play an important role in the fields of soil structure formation, soil nutrient activation, plant nutrient absorption, biological pollution stress, environmental pollution remediation and the like, and are generally concerned by scholars at home and abroad. The commonly used method for collecting root exudates at present comprises a solution culture collection method, a substrate culture (vermiculite culture, sand culture, agar culture and the like) collection method and a soil culture collection method. The three collection methods can effectively collect plant root secretions, but have respective defects. The solution culture collection method and the matrix culture collection method can accurately analyze the composition of the root exudates, but both change the normal growth environment of the plants, cannot reduce and reflect the actual secretion condition of the plants in the soil, and the test result is often greatly different from the actual condition of the root exudates in the soil. Although the soil culture collection method can reduce the original growth condition of plants, the organic matters of the soil are difficult to be distinguished from the organic matters secreted by root systems due to the existence of complex compounds in the soil, so that the reliability of research results is influenced. Meanwhile, the existing root secretion collecting device does not systematically compare the root secretion conditions of the same plant in different culture systems, and the understanding of the differences of the plant root secretion characteristics in different culture environments is limited. Therefore, the realization of in-situ collection and accurate collection of root exudates on the premise of not damaging root growth is always a technical problem in academia.

In recent years, the application of stable isotope labeling technology provides an important way for the research of rhizosphere ecology. Wherein¹³CO₂The plant (indirect) marking method plays an important role in rhizosphere ecology research, and can track the flow of carbon in a plant-soil system so as to evaluate the influence of environmental conditions on carbon flow. At present, utilize¹³CO₂The culture and collection device for researching plant root exudates by a plant (indirect) marking method is less, and especially, the in-situ collection and the accurate collection of the root exudates are not realized on the premise of not damaging the growth of the root system.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to overcome prior art not enough, provide a plant root system secretion collection device, can distinguish the organic matter of soil self with root system secretion, improve the reliability of result, root system secretion distribution characteristic in the different rhizosphere minidomains of while can also accurate positioning.

The purpose of the utility model is realized through the following technical scheme:

shared by water and soil environment¹³CO₂The marker culture-root secretion collecting device comprises an upper marker box and a lower plant root box; the marking box comprises a glass cover, an air mixing device and a marking box body which are sequentially communicated, wherein the marking box body is provided with an air valve¹³CO₂The side surface of the glass cover is provided with an air outlet and an air inlet so as to mix air with the air through an air mixing device¹³CO₂Gas input by the gas cylinder is mixed and then input into the glass cover through the gas inlet; the plant root box is divided into a left hydroponic culture chamber and a right soil culture chamber through a partition plate, a plurality of longitudinal partition layers are separated into the right soil culture chamber by nylon nets at intervals, and Micro Rhizon rhizosphere solution samplers are respectively embedded in each longitudinal partition layer.

Further, the marking box is made of organic glass with good light transmittance.

Further, the plant root box and the partition plate are made of PVC materials.

Further, the mesh of the nylon net is less than 25 μm, the width of each compartment is 10mm, and the width of each of the left hydroponic chamber and the right soil culture chamber is 12 cm.

The utility model has the advantages that:

the utility model provides a plant roots secretion collection device has simple structure, area is little and characteristics with low costs, can realize under the prerequisite that does not destroy the root system growth that the organic matter of soil self parts with the root system secretion to root system secretion distribution characteristic in the different rhizosphere miniareas of accurate positioning and survey.

Drawings

Figure 1 is that the utility model provides a pair of water and soil environment sharing¹³CO₂Marker culture-rootIs a schematic structural diagram of a secretion collecting device.

Reference numerals: 1-a glass cover; 2-air outlet; 3-Y type root-dividing tube; 4-left hydroponic culture chamber; 5-right soil culture room; a 6-Micro Rhizon rhizosphere solution sampler; 7-an air inlet; 8-air mixing device, 9-¹³CO₂A gas cylinder.

Detailed Description

The technical solution of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. Based on the embodiments in the present invention, all other embodiments obtained by those skilled in the art without creative efforts belong to the protection scope of the present invention.

Example 1

As shown in figure 1, a plant used in water and soil environment¹³CO₂The marker root system secretion collecting integrated device comprises an upper marker box and a lower plant root box. In the illustrated example, the glass cover 1 of the marking box is formed by bonding 5 organic glass plates with good light transmittance, and the plant root box is made of black PVC plates. An air outlet 2 is arranged at the center of the left side of the glass cover 1, and the center of the right side of the glass cover 1 is connected with an air mixing device 8 through an air inlet 7; the upper part of the air mixing device 8 is connected¹³CO₂And a gas cylinder 9. The plant root box is divided into a water culture chamber 4 and a soil culture chamber 5 by PVC plates from left to right by meshes<The 25 μm nylon net separates the soil culture chamber 5 into 12 longitudinal partitions (0-10mm near rhizosphere soil and>10mm of rhizosphere soil), the width of the left plant culture room and the width of the right plant culture room are respectively 12cm, and Micro rhizozone rhizosphere solution samplers 6 are respectively embedded in each interlayer so as to realize the control of rhizosphere soil (root growth room), 0-10mm of near rhizosphere soil and far rhizosphere soil ((root growth room)>10mm) were collected separately. The Micro Rhizon rhizosphere solution sampler is manufactured by the company of the Netherlands, the model is 19.21.82Micro Rhizon, the diameter is 1mm, the length is 8mm, a sampling head, a PEEL tube and an injector connecting joint thereof, and the sampling amount of the solution is about 2ml generally.

The utility model provides a plant¹³CO₂The device for collecting the root exudates shared by the marking water and soil environments has the characteristics of simple structure, small occupied area and low cost, can separate the organic matters of the soil and the root exudates on the premise of not damaging the growth of the roots, and accurately positions and measures the distribution characteristics of the root exudates in different rhizosphere micro-domains.

DETAILED DESCRIPTION OF EMBODIMENT (S) OF INVENTION

The glass cover 1 of the marking box is formed by bonding 5 organic glass plates with good light transmittance, and the plant root box is processed by adopting a black PVC plate. An air outlet 2 is arranged at the center of the left side of the glass cover 1, and the center of the right side of the glass cover 1 is connected with an air mixing device 8 through an air inlet 7; the upper part of the air mixing device 8 is connected¹³CO₂And a gas cylinder 9.

The plant root box is divided into a left hydroponic culture chamber 4 and a right soil culture chamber 5 from left to right by PVC plates, the right soil culture chamber 5 is separated into 12 longitudinal interlayers (0-10mm near rhizosphere soil and >10mm far rhizosphere soil) by a nylon net with meshes <25 mu m at intervals of 10mm, the widths of the left plant culture chamber and the right plant culture chamber are respectively 12cm, and Micro Rhizon rhizosphere solution samplers 6 are respectively embedded in each interlayer so as to respectively collect the rhizosphere soil (root system growth chamber), the 0-10mm near rhizosphere soil and the far rhizosphere soil (>10 mm).

The Micro Rhizon rhizosphere solution sampler is manufactured by the company of the Netherlands, the model is 19.21.82Micro Rhizon, the diameter is 1mm, the length is 8mm, a sampling head, a PEEL tube and an injector connecting joint thereof, and the sampling amount of the solution is about 2ml generally.

During the use, divide root system of plant through Y type root-dividing pipe 3 evenly, cultivate respectively in left hydroponic room 4 and right soil culture room 5, cover glass cover 1 (mark case) on the root box of plant. Injecting the product with purity of 99% by gas cylinder in daytime¹³CO₂The plant is injected once every 1 hour, 8 times daily, and labeled at a ratio of 40mL (total injection volume)/10L (labeled box volume) per day, so that the plant can be absorbed by photosynthesis¹³CO₂Part of the produced assimilation products are transferred from the overground part to the underground part and then secreted into the rhizosphere soil, so that the root exudates can be tracked in the plant-soilThe flow in the system and the distribution characteristics of the rhizosphere soil, the near rhizosphere soil and the far rhizosphere soil. Injection of¹³CO₂After that, the air mixing device 2 is opened to inject¹³CO₂Mix well with the air and then turn off the air mixing device 8. Collecting the soil solution in the right soil culture chamber 5 after continuously marking for 10 days; the culture solution in the left hydroponic culture chamber 4 was changed every 2 or 3 days, and the changed culture solutions were combined to obtain a total solution. Freezing and concentrating the sample to be powdery substance by a freeze dryer, adding 4ml of ultrapure water, and carrying out ultrasonic treatment for 5min by ultrasonic waves to assist dissolution; Sep-Pak C with small solid phase extraction column₁₈And filtering impurities. The composition characteristics of the root exudates of the soil solution are determined by adopting HPLC, and the gas chromatography-combustion furnace-isotope mass spectrometry (GC-C-IRMS) is utilized to determine the contents of various root exudates¹³Abundance of C (. delta.)¹³C) So that the type of root exudates and the distribution characteristics of different areas of the rhizosphere can be determined.

The foregoing is illustrative of the preferred embodiments of the present invention, and it is to be understood that the invention is not limited to the precise forms disclosed herein, and that various other combinations, modifications, and environments may be resorted to, falling within the scope of the invention as defined by the appended claims. But that modifications and variations may be effected by those skilled in the art without departing from the spirit and scope of the invention, which is to be limited only by the claims appended hereto.

Claims (4)

1. Shared by water and soil environment¹³CO₂Marker cultivation-root system secretion collection device, its characterized in that: comprises a marking box at the upper part and a plant root box at the lower part, wherein the marking box comprises a glass cover (1), an air mixing device (8) and a device provided with an air valve which are sequentially communicated¹³CO₂The gas cylinder (9), the side of the glass cover (1) is provided with a gas outlet (2) and a gas inlet (7), and the gas outlet (2) and the gas inlet (7) are connected through an air mixing device (8)¹³CO₂Gas input by the gas bottle (9) is mixed and then input into the glass cover (1) through the gas inlet (7); the plant root box is divided into a left hydroponic culture chamber (4) and right soil by a PVC plate from left to rightTwo culture areas of the culture room (5), a plurality of longitudinal partition layers which are separated by nylon nets at intervals of 10mm in the right soil culture room (5), and Micro Rhizon rhizosphere solution samplers (6) are respectively embedded in each longitudinal partition layer.

2. The water and soil environment shared according to claim 1¹³CO₂The device for collecting the marker culture root secretion is characterized in that the glass cover (1) is made of transparent organic glass.

3. The water and soil environment shared according to claim 1¹³CO₂Marker cultivation-root system secretion collection device, its characterized in that: the plant root box is made of PVC materials.

4. The water and soil environment shared according to claim 1¹³CO₂Marker cultivation-root system secretion collection device, its characterized in that: the mesh of the nylon net is smaller than 25 mu m, the width of each compartment is 10mm, and the width of the left hydroponic chamber (4) and the width of the right soil culture chamber (5) are respectively 12 cm.

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