CN214612551U

CN214612551U - Multilayer cell culture bottle device

Info

Publication number: CN214612551U
Application number: CN202120804507.8U
Authority: CN
Inventors: 范丽丽; 张爱华; 王大朋; 赵华; 陈雄
Original assignee: Guizhou Medical University
Current assignee: Guizhou Medical University
Priority date: 2021-04-20
Filing date: 2021-04-20
Publication date: 2021-11-05
Anticipated expiration: 2031-04-20

Abstract

The utility model belongs to the technical field of cell culture, and discloses a multilayer cell culture bottle device, which comprises a culture bottle shell, wherein the inner wall of the culture bottle shell is provided with a connecting strip, one side of the connecting strip is provided with a supporting plate, the top of the supporting plate is provided with a convex edge, one side of the top of the culture bottle shell is provided with a culture bottle mouth, the top of the culture bottle mouth is provided with a culture bottle cap, one side of the culture bottle shell, which is opposite to the culture bottle mouth, is provided with a mounting groove, the inside of the mounting groove is provided with a rubber plug, one side of the supporting plate, which is close to the mounting groove, is provided with a chute, the inside of the chute is provided with a baffle plate, the utility model is provided with the mounting groove and the rubber plug, the screw plug in the mounting groove is screwed out, the needle head is inserted into the rubber plug, and a proper amount of culture solution is injected into the surface of the supporting plate, so that the liquid can be conveniently replenished into the multilayer culture bottle by a worker, thereby ensuring the normal growth and reproduction of cells.

Description

Multilayer cell culture bottle device

Technical Field

The utility model belongs to the technical field of cell culture, concretely relates to multilayer cell culture bottle device.

Background

In the process of cell culture, cells need to be placed in a culture solution to simulate an in vivo environment, so that the cells grow and propagate in the external environment, and some mammalian cells need surface adherent culture, so that a culture bottle providing an in vitro cell culture environment is needed, and the cells can be ensured to normally survive and propagate.

In the prior art, the multilayer culture bottle is inconvenient for workers to supplement liquid when in use, so that the survival rate of cells in the culture solution is reduced, and after a proper amount of culture solution is poured into the multilayer culture bottle, the volume of the culture solution on the surface of each layer of supporting plate cannot be guaranteed to be the same or similar, so that the cell culture effect is reduced.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide a multilayer cell culture bottle device to solve current multilayer blake bottle and can not make things convenient for the staff fluid infusion, and the blake bottle can not guarantee that the culture solution volume on every layer is the same or close problem.

In order to achieve the above object, the utility model provides a following technical scheme: the utility model provides a multilayer cell culture bottle device, includes the blake bottle shell, the inner wall of blake bottle shell is provided with the connecting strip, the backup pad is installed to one side of connecting strip, the top of backup pad is provided with the chimb, the cultivation bottleneck has been seted up to top one side of blake bottle shell, the top of cultivateing the bottleneck is provided with cultivates the bottle lid, the blake bottle shell has seted up the mounting groove in one side of cultivateing the bottleneck mutually, the internally mounted of mounting groove has the plug.

Preferably, a sliding groove is formed in one side, close to the mounting groove, of the supporting plate, and a baffle is arranged inside the sliding groove.

Preferably, the inner wall of the culture bottle shell is provided with a reinforcing rib, and the reinforcing rib is fixedly connected with the top of the supporting plate.

Preferably, one side of the rubber plug is provided with a plug screw, and the plug screw is screwed with the inner wall thread of the mounting groove.

Preferably, the sliding blocks are installed on two sides of the baffle and matched with the inner wall of the sliding groove.

Preferably, the second lug is installed to the side inner wall that the blake bottle shell is close to the mounting groove, first lug is installed to the side that the baffle is close to the blake bottle shell, first lug cooperatees with the second lug.

Compared with the prior art, the utility model, following beneficial effect has:

(1) the utility model discloses set up mounting groove and plug, twisted out through the plug screw in the mounting groove to insert the plug through the syringe needle, pour into appropriate amount culture solution into to the surface of backup pad, can make things convenient for the staff to carry out the fluid infusion in to the multilayer blake bottle like this, thereby guarantee the normal growth and the reproduction of cell.

(2) The utility model discloses set up spout, baffle, first lug and second lug, the baffle passes through the slider and slides at the inner wall of spout for the baffle drives first lug and slides to the position with second lug matched with, and equally divide the culture solution of blake bottle shell bottom equally, and the culture solution volume that can effectual assurance backup pad surface like this is close.

Drawings

Fig. 1 is a schematic structural view of the present invention;

FIG. 2 is an enlarged view taken at A in FIG. 1;

FIG. 3 is an enlarged view of FIG. 1 at B;

in the figure: 1. culturing the bottle shell; 2. a support plate; 3. reinforcing ribs; 4. culturing the bottle mouth; 5. culturing a bottle cap; 6. a convex edge; 7. a connecting strip; 8. a chute; 9. a baffle plate; 10. a slider; 11. a first bump; 12. a second bump; 13. mounting grooves; 14. a rubber plug; 15. and (4) a screw plug.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-3, the present invention provides the following technical solutions: the utility model provides a multilayer cell culture bottle device, including blake bottle shell 1, the inner wall of blake bottle shell 1 is provided with connecting strip 7, connecting strip 7 can be stably fixed with backup pad 2, backup pad 2 is installed to one side of connecting strip 7, the top of backup pad 2 is provided with chimb 6, chimb 6 can effectually prevent that the culture solution on backup pad 2 surface from spilling over, it has seted up cultivation bottleneck 4 to cultivate top one side of blake bottle shell 1, the top of cultivateing bottleneck 4 is provided with cultivates bottle lid 5, it can be effectual that cultivation bottleneck 4 is sealed to cultivate bottle lid 5, it has seted up mounting groove 13 to cultivate bottle shell 1 one side of carrying on the back in cultivation bottleneck 4, the internally mounted of mounting groove 13 has plug 14, plug 14 can make things convenient for the staff to insert the needle tubing in blake bottle shell 1, and the fluid infusion.

Further, the supporting plate 2 is close to one side of the mounting groove 13 and is provided with a sliding groove 8, a baffle plate 9 is arranged inside the sliding groove 8, and the baffle plate 9 can equally divide the culture solution injected into the culture bottle shell 1.

Furthermore, the reinforcing rib 3 is installed to the inner wall of blake bottle shell 1, and the top fixed connection of reinforcing rib 3 and backup pad 2, reinforcing rib 3 can increase the stability of backup pad 2, prevents that the blake bottle warp.

Specifically, one side of plug 14 is provided with plug screw 15, and plug screw 15 closes with the inner wall screw thread of mounting groove 13 soon and is connected, can prevent during outside air gets into the blake bottle, makes things convenient for the staff to carry out the fluid infusion to the culture solution simultaneously.

It is worth to say that, the slider 10 is all installed to the both sides of baffle 9, and slider 10 cooperatees with the inner wall of spout 8, and slider 10 can make things convenient for baffle 9 to slide in spout 8, evenly divides the culture solution equally.

Further, the second lug 12 is installed to one side inner wall that culture bottle shell 1 is close to mounting groove 13, and baffle 9 is close to one side of culture bottle shell 1 and installs first lug 11, and first lug 11 cooperatees with second lug 12, and first lug 11 and second lug 12 can prevent that the equal cell culture liquid that divides equally fuses each other.

The utility model discloses a theory of operation and use flow: when the utility model is used, the culture solution containing cells is poured into the culture bottle shell 1 through the culture bottle mouth 4, when a proper amount of culture solution containing cells is poured, the culture bottle shell 1 is wholly and slowly erected, the mounting groove 13 is attached to the tabletop, the culture solution is positioned at the bottom of the culture bottle shell 1, the baffle 9 slides downwards in the chute 8 through the slider 10, so that the culture solution in the culture bottle shell 1 is evenly separated, the culture bottle shell 1 is wholly and slowly inclined and is placed horizontally, and therefore, equal culture solution is arranged on the surface of each layer of the supporting plate 2;

after the culture solution is through cold-stored, need carry out the fluid infusion to the culture solution, rotate out screw plug 15 from mounting groove 13, insert the fluid infusion syringe needle again in plug 14 to extend to the upper surface of backup pad 2, carry out the fluid infusion again, thereby guarantee cell culture's accuracy.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A multi-layered cell culture flask device, characterized in that: including blake bottle shell (1), the inner wall of blake bottle shell (1) is provided with connecting strip (7), backup pad (2) are installed to one side of connecting strip (7), the top of backup pad (2) is provided with chimb (6), the top one side of blake bottle shell (1) has been seted up and has been cultivateed bottleneck (4), the top of cultivateing bottleneck (4) is provided with cultivates bottle lid (5), install groove (13) have been seted up in one side of blake bottle shell (1) back on the body in cultivation bottleneck (4), the internally mounted of install groove (13) has plug (14).

2. The multi-layered cell culture flask device of claim 1, wherein: one side of the supporting plate (2) close to the mounting groove (13) is provided with a sliding groove (8), and a baffle plate (9) is arranged inside the sliding groove (8).

3. The multi-layered cell culture flask device of claim 1, wherein: reinforcing ribs (3) are installed on the inner wall of the culture bottle shell (1), and the reinforcing ribs (3) are fixedly connected with the top of the supporting plate (2).

4. The multi-layered cell culture flask device of claim 1, wherein: one side of the rubber plug (14) is provided with a screw plug (15), and the screw plug (15) is screwed with the inner wall thread of the mounting groove (13).

5. The multi-layered cell culture flask assembly of claim 2, wherein: and sliding blocks (10) are mounted on two sides of the baffle (9), and the sliding blocks (10) are matched with the inner wall of the sliding groove (8).

6. The multi-layered cell culture flask assembly of claim 2, wherein: second lug (12) are installed to one side inner wall that blake bottle shell (1) is close to mounting groove (13), baffle (9) are close to one side of blake bottle shell (1) and install first lug (11), first lug (11) cooperate with second lug (12).