CN214612425U - A DNA enrichment facility for court science - Google Patents
A DNA enrichment facility for court science Download PDFInfo
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- CN214612425U CN214612425U CN202120704847.3U CN202120704847U CN214612425U CN 214612425 U CN214612425 U CN 214612425U CN 202120704847 U CN202120704847 U CN 202120704847U CN 214612425 U CN214612425 U CN 214612425U
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Abstract
The utility model provides a DNA concentration device used in court science, which comprises a concentration tube, a vacuum tube, a nano-filtration membrane, an installation unit and a sealing connection unit; the upper end and the lower end of the concentration pipe are communicated with a detachable nano-filtration membrane through an installation unit, and the installation unit is connected with the vacuum pipe through a sealing connection unit; the installation unit is including the locking structure, first sealing washer, an installation section of thick bamboo, the fixture block, the installation boss, installation section of thick bamboo upper end and the lower extreme slip registrate of concentrated pipe, and the upper end inner wall of an installation section of thick bamboo is fixed with a plurality of fixture blocks, the sealed installation boss that is fixed with of the lower extreme inner wall of an installation section of thick bamboo, nanofiltration membrane detachable sets up the up end at the installation boss, the lower terminal surface of installation boss is located the coplanar and is connected with the vacuum tube through sealed coupling unit with the lower terminal surface of an installation section of thick bamboo, still be provided with the first sealing washer of detachable between nanofiltration membrane up end and the concentrated pipe lower terminal surface, the fixture block cooperates with the locking structure of setting on concentrated pipe outer wall.
Description
Technical Field
The utility model relates to a enrichment facility field, especially a DNA enrichment facility for court science.
Background
Concentration is the operation of removing part of the solvent (usually water) from the solution, and is also part of the separation process of the homogeneous mixture of solute and solvent. The method can remove a large amount of water in the solution by concentration, reduce the mass and the volume, improve the concentration of the solution, and obtain a pure DNA solution with higher concentration in the DNA extraction process, which is the key for carrying out DNA-PCR amplification. The discovery, transfer and extraction of biological material evidence are the key points in forensic medicine, in order to extract pure DNA solution with high template amount, the separation and purification method can play the roles of purification and concentration, the existing method is ethanol precipitation, a magnetic bead method, a silicon bead method, an automatic workstation method and an ultrafiltration method, and the advantages and the disadvantages of various methods, such as loss of adsorption rate, elution volume and the like. The concentration of DNA solution with low volume such as 30 microliter is a problem of current research, and the prior art generally adopts nitrogen blower, heating evaporation and other methods to concentrate the DNA solution, but the possible problems of DNA molecule drift and pollution exist.
SUMMERY OF THE UTILITY MODEL
In order to solve the above problems, the present invention provides a DNA concentration device for forensic science, wherein a concentration tube and a vacuum tube are hermetically connected by a mounting unit and a sealing connection unit, so that the DNA solution is concentrated by vacuum.
In order to achieve the above purpose, the utility model adopts the following technical scheme: a DNA concentration device for forensic science comprises a concentration tube, a vacuum tube, a nano-filtration membrane, a mounting unit and a sealing connection unit;
the concentration pipe is communicated up and down, the lower end of the concentration pipe is provided with a detachable nano-filtration membrane through an installation unit, and the installation unit is connected with the vacuum pipe through a sealing connection unit;
the installation unit is including locking structure, first sealing washer, installation section of thick bamboo, fixture block, mount table, installation section of thick bamboo upper end and the lower extreme slip registrate of concentrated pipe, and the upper end inner wall of installation section of thick bamboo is fixed with a plurality of fixture blocks, the sealed mount table that is fixed with of the lower extreme inner wall of installation section of thick bamboo, nanofiltration membrane detachable sets up the up end at the mount table, a plurality of trompils have been seted up on the mount table, the lower terminal surface of mount table is located the coplanar and is connected with the vacuum tube through sealing connection unit with the lower terminal surface of installation section of thick bamboo, still be provided with the first sealing washer of detachable between nanofiltration membrane up end and the concentrated pipe lower terminal surface, the fixture block cooperates with the locking structure that sets up on concentrated pipe outer wall.
Furthermore, the locking structure comprises a first sliding chute, a second sliding chute, a third sliding chute, a limiting block, a sliding rod, a baffle, a spring, a mounting plate, a handle and a through hole;
the outer wall of the concentration pipe is provided with a first sliding chute and a second sliding chute which are in sliding fit with the clamping block, the first sliding chute is arranged along the length direction of the concentration pipe, one end of the first sliding chute is communicated with the lower end surface of the concentration pipe, the other end of the first sliding chute is communicated with the lower end of the second sliding chute which is obliquely arranged, a through hole is arranged on one side wall of the high end of the second sliding chute, a third sliding chute is also arranged on the outer wall of the concentration pipe along the length direction of the concentration pipe, the third sliding groove is positioned below the second sliding groove, a mounting plate is fixed in the third sliding groove, the limiting block is arranged in the through hole in a sliding manner and is fixed at one end of the sliding rod, the other end of the sliding rod penetrates through the mounting plate in a sliding manner, is positioned in the third sliding chute and is fixedly provided with a handle, the spring is sleeved on the outer wall of the sliding rod, and one end is linked with the baffle fixed on the sliding rod, the other end is connected with the upper end face of the mounting plate, and one side of the limiting block close to the first sliding groove is provided with a guide inclined plane.
Furthermore, the sealing connection unit comprises a connection cylinder, a second sealing ring and a fastening structure;
one end of the connecting cylinder is connected with the lower end face of the mounting cylinder in a sealing mode, the other end of the connecting cylinder is sleeved with the vacuum tube, the second sealing ring is arranged in the connecting cylinder and fixed to the lower end face of the mounting table, and a fastening structure connected with the vacuum tube is fixed to the lower end face of the connecting cylinder.
Furthermore, the fastening structure comprises a first hinged plate, a second hinged plate, a first torsion spring, a second torsion spring, a limiting rod, a fixed plate and a rotating rod;
the utility model discloses a vacuum tube, including a connecting cylinder, first articulated slab, second articulated slab, first torsion spring, second torsional spring, first articulated slab, second articulated slab, the last terminal surface of first articulated slab articulates the lower terminal surface at the connecting cylinder, first articulated slab one end is stretched out the connecting cylinder and is fixed with the bull stick, and the other end is articulated with second articulated slab one end, in the second articulated slab stretches into the connecting cylinder, first torsion spring one end is connected with first articulated slab up end, and the other end and connecting cylinder outer wall connection, second torsion spring one end is connected with second articulated slab up end, and the other end is connected with first articulated slab lateral wall, the terminal surface is fixed with the gag lever post that is used for spacing second articulated slab under the first articulated slab, the cooperation of vacuum tube outer wall and second articulated slab is fixed to the fixed plate.
Furthermore, a connecting pipe used for being connected with a vacuum pump is also arranged on the vacuum pipe.
Due to the adoption of the technical scheme, the utility model discloses following advantage has:
1. the installation table convenient for placing the nano-filtration membrane is arranged in the installation cylinder, and the first sealing ring is arranged on the nano-filtration membrane, so that the nano-filtration membrane can be conveniently replaced, resources are saved, and the cost is reduced;
2. the installation cylinder provided with the nano-filtration membrane seals the nano-filtration membrane on the lower end face of the concentration tube through the first sliding chute and the second sliding chute under the action of the fixture block by arranging the first sliding chute and the second sliding chute on the outer wall of the installation cylinder, and the installation cylinder is fixed under the action of the limiting block, the sliding rod and the spring, so that the installation and disassembly are convenient, the structure is simple, and the operation is simple and convenient;
3. the concentrator tube with the fixed nanofiltration membrane is sleeved on the outer wall of the vacuum tube and is connected with the vacuum tube in a sealing mode through pressing under the action of the connecting cylinder, the second hinged plate, the second torsion spring and the fixing plate fixed on the outer wall of the vacuum tube, and the concentrator tube is simple in structure and convenient to operate.
Additional advantages, objects, and features of the invention will be set forth in part in the description which follows and in part will become apparent to those having ordinary skill in the art upon examination of the following or may be learned from practice of the invention. The objectives and other advantages of the present invention will be realized and attained by the structure particularly pointed out in the written description and claims hereof.
Drawings
The drawings of the present invention are described below.
Fig. 1 is a schematic structural view of the present invention;
fig. 2 is a cross-sectional view of the present invention;
FIG. 3 is a schematic structural view of the first and second chutes;
FIG. 4 is a schematic structural view of a limiting block and a sliding rod;
FIG. 5 is a schematic structural view of the connector barrel;
fig. 6 is an enlarged view of a in fig. 5.
In the figure: 1-a concentration tube; 2-vacuum tube; 3-nanofiltration membrane; 4-a first sealing ring; 5, mounting a cylinder; 6-clamping blocks; 7-mounting a table; 8-a first chute; 9-a second chute; 10-a third chute; 11-a limiting block; 12-a slide bar; 13-a baffle; 14-a spring; 15-mounting a plate; 16-a handle; 17-a through hole; 18-a connector barrel; 19-a second sealing ring; 20-a first hinge plate; 21-a second hinge plate; 22-a first torsion spring; 23-a second torsion spring; 24-a stop lever; 25-fixing the plate; 26-a rotating rod; 27-connecting tube.
Detailed Description
The present invention will be further explained with reference to the drawings and examples.
As shown in fig. 1 to 6, the present invention provides a DNA concentration device for forensic science, which comprises a concentration tube 1, a vacuum tube 2, a nano-filtration membrane 3, an installation unit, and a sealing connection unit;
the concentration tube 1 is communicated up and down, the lower end of the concentration tube 1 is provided with a detachable nano-filtration membrane 3 through an installation unit, and the installation unit is connected with the vacuum tube 2 through a sealing connection unit;
the mounting unit comprises a locking structure, a first sealing ring 4, a mounting cylinder 5, clamping blocks 6 and a mounting table 7, the upper end of the mounting cylinder 5 is slidably sleeved with the lower end of the concentration pipe 1, the inner wall of the upper end of the mounting cylinder 5 is fixedly provided with the clamping blocks 6, the inner wall of the lower end of the mounting cylinder 5 is fixedly provided with the mounting table 7 in a sealing manner, the nanofiltration membrane 3 is detachably arranged on the upper end surface of the mounting table 7, the mounting table 7 is provided with a plurality of openings, the lower end surface of the mounting table 7 and the lower end surface of the mounting cylinder 5 are positioned on the same plane and connected with the vacuum pipe 2 through a sealing connection unit, the detachable first sealing ring 4 is further arranged between the upper end surface of the nanofiltration membrane 3 and the lower end surface of the concentration pipe 1, and the clamping blocks 6 are matched with the locking structure arranged on the outer wall of the concentration pipe 1;
As a further scheme of the present invention, the locking structure includes a first sliding groove 8, a second sliding groove 9, a third sliding groove 10, a limiting block 11, a sliding rod 12, a baffle 13, a spring 14, a mounting plate 15, a handle 16, and a through hole 17;
a first sliding groove 8 and a second sliding groove 9 which are in sliding fit with a clamping block 6 are arranged on the outer wall of the concentration tube 1, the first sliding groove 8 is arranged along the length direction of the concentration tube 1, one end of the first sliding groove 8 is communicated with the lower end face of the concentration tube 1, the other end of the first sliding groove is communicated with the lower end of the second sliding groove 9 which is obliquely arranged, a through hole 17 is arranged on the side wall of the high end of the second sliding groove 9, a third sliding groove 10 is also arranged on the outer wall of the concentration tube 1 along the length direction of the concentration tube 1, the third sliding groove 10 is positioned below the second sliding groove 9, a mounting plate 15 is fixed in the third sliding groove 10, a limiting block 11 is arranged in the through hole 17 in a sliding manner and fixed at one end of a sliding rod 12, the other end of the sliding rod 12 penetrates through the mounting plate 15 and is positioned in the third sliding groove 10 in a sliding manner and is fixed with a handle 16, a spring 14 is sleeved on the outer wall of the sliding rod 12, and one end of the spring is connected with a baffle 13 fixed on the sliding rod 12, the other end of the limiting block is connected with the upper end face of the mounting plate 15, and a guide inclined plane is arranged on one side, close to the first sliding chute 8, of the limiting block 11;
after placing nanofiltration membrane 3, installation section of thick bamboo 5 is at fixture block 6 and first spout 8, under second spout 9 sliding fit's effect, with concentrated pipe 1 sealing connection, fixture block 6 contacts with the direction inclined plane of stopper 11 when second spout 9 internal slipping, thereby slide stopper 11 in through-hole 17, thereby compress spring 14, after stopper 11 breaks away from with fixture block 6, stretch out through-hole 17 under the effect that spring 14 resets and fix fixture block 6 in second spout 9 and the second spout 9 that the slope was seted up makes installation section of thick bamboo 5 and concentrated pipe 1 installation more firm, when needs dismantle installation section of thick bamboo 5, through 16 downwards pulling slide bar 12 of handle, stopper 11 is not to fixture block 6 fibre, fixture block 6 roll-off second spout 9.
As a further aspect of the present invention, the sealing connection unit includes a connection cylinder 18, a second sealing ring 19, and a fastening structure;
one end of the connecting cylinder 18 is connected with the lower end face of the mounting cylinder 5 in a sealing manner, the other end of the connecting cylinder is sleeved with the vacuum tube 2, the second sealing ring 19 is arranged in the connecting cylinder 18 and fixed on the lower end face of the mounting table 7, and a fastening structure connected with the vacuum tube 2 is fixed on the lower end face of the connecting cylinder 18.
As a further aspect of the present invention, the fastening structure includes a first hinged plate 20, a second hinged plate 21, a first torsion spring 22, a second torsion spring 23, a limiting rod 24, a fixing plate 25, and a rotating rod 26;
the upper end surface of the first hinged plate 20 is hinged to the lower end surface of the connecting cylinder 18, one end of the first hinged plate 20 extends out of the connecting cylinder 18 and is fixedly provided with a rotating rod 26, the other end of the first hinged plate 20 is hinged to one end of the second hinged plate 21, the second hinged plate 21 extends into the connecting cylinder 18, one end of the first torsion spring 22 is connected with the upper end surface of the first hinged plate 20, the other end of the first torsion spring is connected with the outer wall of the connecting cylinder 18, one end of the second torsion spring 23 is connected with the upper end surface of the second hinged plate 21, the other end of the second torsion spring is connected with the side wall of the first hinged plate 20, the lower end surface of the first hinged plate 20 is fixedly provided with a limiting rod 24 for limiting the second hinged plate 21, and the fixing plate 25 is fixed on the outer wall of the vacuum tube 2 and is matched with the second hinged plate 21;
after the nano-filtration membrane 3 is fixed at the lower end of the concentration tube 1 through the mounting cylinder 5, the water outlet of the concentration tube 1 is hermetically connected with the water inlet of the vacuum tube 2 through a first hinged plate 20 which is hinged to the lower end surface of a connecting cylinder 18 fixed at the lower end surface of the fixing cylinder 5 and a second hinged plate 21 which is hinged to the first hinged plate 20.
As a further aspect of the present invention, the vacuum tube 2 is further provided with a connecting tube 27 for connecting with a vacuum pump.
The working principle is as follows: when in use, the nano-filtration membrane 3 is placed on the upper end surface of the mounting table 7, the first sealing ring 4 is arranged on the upper end surface of the nano-filtration membrane 3, the mounting cylinder 5 on which the nano-filtration membrane 3 is placed slides along the first sliding groove 8 and the second sliding groove 9 under the action of the fixture block 6, the mounting cylinder 5 is sealed and fixed through the limiting block 11, the mounting cylinder 5 on which the concentration tube 1 is mounted is pressed through the connecting cylinder 18 fixed on the lower end surface, the first hinged plate 20 and the second hinged plate 21 on the lower end surface of the connecting cylinder 18, the connecting cylinder 18 is sleeved on the upper end of the vacuum tube 2, the fixing plate 25 is contacted with the lower end surface of the second hinged plate 21 and presses the second hinged plate 21 to compress the second torsion spring 23, the lower end surface of the second hinged plate 21 is reset under the action of the second torsion spring 23 after being separated from the fixing plate 25, the upper end surface of the second hinged plate 21 is contacted with the fixing plate 25, and the concentration tube 1 is sealed and connected with the vacuum tube 2 under the limiting action of the limiting rod 24, then adding the DNA solution to be concentrated into the concentration tube 1, communicating the vacuum tube 2 with a vacuum pump for DNA concentration, rotating the first hinge plate 20 through the rotating rod 26 to compress the first torsion spring 22 after the DNA concentration is finished, separating the second hinge plate 21 from the fixing plate 25, and taking out the concentration tube 1.
Finally, the above embodiments are only used for illustrating the technical solutions of the present invention and not for limiting, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications or equivalent substitutions may be made to the technical solutions of the present invention without departing from the spirit and scope of the technical solutions, and all of them should be covered by the scope of the claims of the present invention.
Claims (5)
1. The DNA concentration device for forensic science is characterized by comprising a concentration tube (1), a vacuum tube (2), a nano-filtration membrane (3), a mounting unit and a sealing connection unit;
the concentrating pipe (1) is communicated up and down, the lower end of the concentrating pipe (1) is provided with a detachable nano-filtration membrane (3) through an installation unit, and the installation unit is connected with the vacuum pipe (2) through a sealing connection unit;
the mounting unit comprises a locking structure, a first sealing ring (4), a mounting cylinder (5), a clamping block (6) and a mounting platform (7), the upper end of the mounting cylinder (5) is sleeved with the lower end of the concentration pipe (1) in a sliding manner, a plurality of clamping blocks (6) are fixed on the inner wall of the upper end of the mounting cylinder (5), a mounting platform (7) is fixed on the inner wall of the lower end of the mounting cylinder (5) in a sealing way, the nano-filtration membrane (3) is detachably arranged on the upper end surface of the mounting table (7), the mounting table (7) is provided with a plurality of openings, the lower end surface of the mounting table (7) and the lower end surface of the mounting cylinder (5) are positioned on the same plane and are connected with the vacuum tube (2) through a sealing connection unit, a detachable first sealing ring (4) is arranged between the upper end surface of the nano filtering membrane (3) and the lower end surface of the concentration tube (1), the clamping block (6) is matched with a locking structure arranged on the outer wall of the concentration pipe (1).
2. The DNA concentration device for forensic science according to claim 1, wherein the locking structure comprises a first sliding groove (8), a second sliding groove (9), a third sliding groove (10), a stopper (11), a sliding rod (12), a baffle (13), a spring (14), a mounting plate (15), a handle (16), and a through hole (17);
a first sliding groove (8) and a second sliding groove (9) which are in sliding fit with the clamping block (6) are formed in the outer wall of the concentration pipe (1), the first sliding groove (8) is formed in the length direction of the concentration pipe (1), one end of the first sliding groove is communicated with the lower end face of the concentration pipe (1), the other end of the first sliding groove is communicated with the lower end of the second sliding groove (9) which is formed in an inclined mode, a through hole (17) is formed in the side wall of the high end of the second sliding groove (9), a third sliding groove (10) is further formed in the outer wall of the concentration pipe (1) in the length direction of the concentration pipe (1), the third sliding groove (10) is located below the second sliding groove (9), a mounting plate (15) is fixed in the third sliding groove (10), a limiting block (11) is arranged in the through hole (17) in a sliding mode and fixed at one end of a sliding rod (12), the other end of the sliding rod (12) penetrates through the mounting plate (15) to be located in the third sliding groove (10) and is fixed with a handle (16), spring (14) cover is established on slide bar (12) outer wall, and one end and baffle (13) of fixing on slide bar (12) are linked, and the other end is connected with mounting panel (15) up end, one side that stopper (11) are close to first spout (8) is provided with the direction inclined plane.
3. The DNA concentration apparatus for forensic science according to claim 1, wherein the sealing connection unit comprises a connection cylinder (18), a second sealing ring (19), a fastening structure;
one end of the connecting cylinder (18) is connected with the lower end face of the mounting cylinder (5) in a sealing mode, the other end of the connecting cylinder is sleeved with the vacuum tube (2), the second sealing ring (19) is arranged in the connecting cylinder (18) and fixed to the lower end face of the mounting table (7), and a fastening structure connected with the vacuum tube (2) is fixed to the lower end face of the connecting cylinder (18).
4. The DNA concentration device for forensic science according to claim 3, wherein the fastening structure comprises a first hinged plate (20), a second hinged plate (21), a first torsion spring (22), a second torsion spring (23), a limiting rod (24), a fixed plate (25), a rotating rod (26);
the upper end face of the first hinged plate (20) is hinged to the lower end face of the connecting cylinder (18), one end of the first hinged plate (20) extends out of the connecting cylinder (18) and is fixed with a rotating rod (26), the other end of the first hinged plate is hinged to one end of the second hinged plate (21), the second hinged plate (21) extends into the connecting cylinder (18), one end of a first torsion spring (22) is connected with the upper end face of the first hinged plate (20), the other end of the first torsion spring is connected with the outer wall of the connecting cylinder (18), one end of a second torsion spring (23) is connected with the upper end face of the second hinged plate (21), the other end of the second torsion spring is connected with the side wall of the first hinged plate (20), a limiting rod (24) used for limiting the second hinged plate (21) is fixed on the lower end face of the first hinged plate (20), and a vacuum tube fixing plate (25) is fixed on the outer wall of the first hinged plate (2) and matched with the second hinged plate (21).
5. The apparatus for concentrating DNA used in forensic science according to claim 4 characterized in that the vacuum tube (2) is further provided with a connection tube (27) for connection to a vacuum pump.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120704847.3U CN214612425U (en) | 2021-04-07 | 2021-04-07 | A DNA enrichment facility for court science |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120704847.3U CN214612425U (en) | 2021-04-07 | 2021-04-07 | A DNA enrichment facility for court science |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214612425U true CN214612425U (en) | 2021-11-05 |
Family
ID=78400265
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120704847.3U Expired - Fee Related CN214612425U (en) | 2021-04-07 | 2021-04-07 | A DNA enrichment facility for court science |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214612425U (en) |
-
2021
- 2021-04-07 CN CN202120704847.3U patent/CN214612425U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211105 |
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| CF01 | Termination of patent right due to non-payment of annual fee |