CN214546164U - Culture medium inoculation mixing arrangement - Google Patents
Culture medium inoculation mixing arrangement Download PDFInfo
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- CN214546164U CN214546164U CN202120188627.XU CN202120188627U CN214546164U CN 214546164 U CN214546164 U CN 214546164U CN 202120188627 U CN202120188627 U CN 202120188627U CN 214546164 U CN214546164 U CN 214546164U
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Abstract
The utility model is suitable for the field of strain inoculation equipment, and provides a culture medium inoculation mixing device, which comprises a sterilizer, wherein the sterilizer is connected with a cooling inoculator through a feed inlet, and the cooling inoculator is provided with a feed opening; the cooling inoculation machine is provided with a temperature cooling mechanism; stirring devices are arranged in the cooling inoculation machine and the sterilization machine, the sterilization machine and the cooling inoculation machine respectively comprise a cylindrical tank body, and the inner diameter of the tank body is gradually reduced from the middle part to two sides; the stirring device comprises a servo motor, the output end of the servo motor is connected with a screw rod, a moving part is in threaded connection with the screw rod, and stirring blades and a cleaning plate are arranged on the moving part; a containing cylinder is arranged on the cleaning plate, a plurality of movable scrapers are arranged in the containing cylinder, and springs are connected between any movable scraper and the bottom wall of the containing cylinder; one end of the movable scraper close to the inner wall of the tank body is provided with a wiping block made of flexible material; the device can avoid the culture medium from adhering inside the device.
Description
Technical Field
The utility model relates to a bacterial inoculation equipment field, concretely relates to culture medium inoculation mixing arrangement.
Background
In order to improve the success rate of inoculation, the culture medium for strain growth and propagation needs to be sterilized at high temperature before being mixed with strains, so that other microorganisms which are not needed in the culture medium are killed, and the culture medium needs to be cooled after being sterilized at high temperature so as to avoid the influence on strain production and propagation due to higher temperature of the culture medium.
Therefore, the Chinese utility model with the patent number of CN201820869685.7 provides sterilization, cooling and inoculation integrated equipment for edible mushroom culture media, and the equipment adopts split operation for high-temperature sterilization and cooling inoculation of the culture media, so that the two are conveniently carried out simultaneously, and the operation efficiency is greatly improved; however, since the medium has a certain viscosity and tends to stick to the inner wall of the sterilizer and the cooling inoculator when they are stirred, there is a need for further improvement of the apparatus.
SUMMERY OF THE UTILITY MODEL
The utility model provides a culture medium inoculation mixing arrangement aims at solving the problem that proposes in the background art.
In order to achieve the above purpose, the utility model adopts the following technical scheme to realize:
a culture medium inoculation mixing device comprises a sterilizer for sterilizing a culture medium, wherein the sterilizer is connected with a cooling inoculation machine through a feed inlet which is controlled to be opened and closed by a feed valve, and the cooling inoculation machine is provided with a feed outlet which is controlled to be opened and closed by a discharge valve; the culture medium enters a sterilizer and then is sterilized at high temperature, then enters a cooling inoculation machine through a feed inlet, the cooling inoculation machine cools the culture medium, and after the strains are mixed with the culture medium in the cooling inoculation machine, the mixture is poured out of the cooling inoculation machine through a feed outlet; the cooling inoculation machine is provided with a temperature cooling mechanism for reducing the temperature in the cooling inoculation machine to the inoculation temperature; stirring devices for stirring the culture medium are arranged in the cooling inoculation machine and the sterilization machine; the stirring device in the sterilizer can uniformly stir the culture medium, so that subsequent inoculation is facilitated, and the stirring device in the cooling inoculator can uniformly mix the stirring medium and the strains; the sterilizer and the cooling inoculator both comprise cylindrical tank bodies, the inner diameters of the tank bodies are gradually reduced from the middle parts to two sides, and the feed inlet and the feed outlet are respectively positioned at the upper and lower positions of the middle parts of the tank bodies; in the stirring process, the culture medium tends to be close to the middle part of the tank body, and simultaneously, the feed inlet and the feed outlet are respectively positioned at the upper part and the lower part of the middle part of the tank body, so that the culture medium is poured out from the feed outlet; the stirring device comprises a servo motor, the servo motor is arranged on the tank body, the output end of the servo motor is connected with a screw rod, the screw rod transversely extends into the tank body, a moving part is in threaded connection with the screw rod, and stirring blades and a cleaning plate are arranged on the moving part; the servo motor is started to drive the screw rod to rotate, so that the moving part is driven to move back and forth along the arrangement direction of the screw rod, and the stirring blades are used for stirring the culture medium; a containing cylinder is arranged on the cleaning plate, a plurality of movable scrapers are mounted in the containing cylinder, and springs are connected between any movable scraper and the bottom wall of the containing cylinder; one end of the movable scraper close to the inner wall of the tank body is provided with a wiping block made of flexible material; because the tank body is not in a regular cylindrical shape, the inner diameter of the tank body is gradually reduced from the middle part to two sides, and therefore, a plurality of movable scraping plates and wiping blocks made of flexible materials are arranged, the part for scraping the inner wall of the tank body is matched with the shape of the tank body as much as possible, and a better scraping effect is achieved.
Further, the sterilizer comprises a vacuum pumping port, a steam inlet and an opening for the medium to enter; the sterilizer is connected with the air extractor through the vacuumizing port before high-temperature sterilization, and steam is injected into the sterilizer through the steam inlet after the sterilizer is vacuumized to perform high-temperature sterilization.
Further, an inoculation spray head for spraying the strains into the cooling inoculation machine is arranged on the cooling inoculation machine; after the temperature of the culture medium in the cooling inoculator is reduced to the inoculation temperature, the strains are injected into the cooling inoculator through the inoculation nozzle for inoculation.
Further, the temperature cooling mechanism comprises a cooling interlayer sleeved on the cooling inoculation machine and a vacuum quick cooling system connected with the cooling inoculation machine through a heat exchanger; the top of the cooling interlayer is provided with a cooling water inlet, and the bottom of the cooling interlayer is provided with a cooling water outlet.
Furthermore, temperature sensors for detecting the temperature of the strains and the temperature of the culture medium are arranged in the sterilizer and the cooling inoculator.
Further, the two ends of the tank body are provided with bearings, and the screw rod is connected with the tank body through the bearings.
The utility model has the advantages that: the device enables the servo motor to drive the stirring blades to stir the culture medium through the matching arrangement between the stirring device and the tank body, and simultaneously drives the movable scraper plate to scrape the culture medium adhered to the inner wall of the tank body; the inner diameter of the tank body is gradually reduced from the middle part to the two sides, so that materials tend to be close to the middle part of the tank body in the stirring process, and the materials are conveniently discharged from a discharging opening below the middle part of the tank body; because the tank body is not in a regular cylindrical shape, the inner diameter of the tank body is gradually reduced from the middle part to two sides, and therefore, a plurality of movable scraping plates and wiping blocks made of flexible materials are arranged, the part for scraping the inner wall of the tank body is matched with the shape of the tank body as much as possible, and a better scraping effect is achieved.
Drawings
FIG. 1 is a schematic view of the present invention;
FIG. 2 is a schematic view of a portion of a stirring device;
fig. 3 is a schematic view of the operation of the cleaning plate portion.
In the figure: 1. a sterilizer; 2. a feed inlet; 3. cooling the inoculation machine; 4. an inoculation spray head; 5. a feeding port; 6. a temperature cooling mechanism; 7. a stirring device; 8. a tank body; 9. a servo motor; 10. a screw rod; 11. a moving part; 12. a stirring blade; 13. cleaning the plate; 14. a movable scraper; 15. a spring; 16. a vacuum pumping port; 17. a steam inlet; 18. an opening; 19. a wiping block.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, a clear and complete description will be given below with reference to the technical solutions of the embodiments of the present invention, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without any inventive step based on the embodiments of the present invention, belong to the scope of protection of the present invention.
As shown in fig. 1-3, a culture medium inoculation mixing device comprises a sterilizer 1 for sterilizing a culture medium, wherein the sterilizer 1 is connected with a cooling inoculation machine 3 through a feed inlet 2 controlled to be opened and closed by a feed valve, and the cooling inoculation machine 3 is provided with a feed outlet 5 controlled to be opened and closed by a discharge valve; the culture medium enters a sterilizer 1 and then is sterilized at high temperature, then the culture medium enters a cooling inoculation machine 3 through a feed inlet 2, the cooling inoculation machine 3 cools the culture medium, and after the strains are mixed with the culture medium in the cooling inoculation machine 3, the mixture is poured out of the cooling inoculation machine 3 through a feed outlet 5; the cooling inoculation machine 3 is provided with a temperature cooling mechanism 6 for reducing the temperature in the cooling inoculation machine 3 to the inoculation temperature; the cooling inoculation machine 3 and the sterilization machine 1 are both internally provided with a stirring device 7 for stirring the culture medium; the stirring device 7 in the sterilizer 1 can uniformly stir the culture medium for subsequent inoculation, and the stirring device 7 in the cooling inoculator 3 can uniformly mix the stirring medium with the strains; the sterilizer 1 and the cooling inoculator 3 both comprise cylindrical tank bodies 8, the inner diameters of the tank bodies 8 are gradually reduced from the middle parts to two sides, and the feed inlet 2 and the feed outlet 5 are respectively positioned at the upper and lower positions of the middle parts of the tank bodies 8; in the stirring process, the culture medium tends to be close to the middle of the tank body 8, and simultaneously, the feed port 2 and the feed port 5 are respectively positioned at the upper and lower positions of the middle of the tank body 8, so that the culture medium can be poured out from the feed port 5; the stirring device 7 comprises a servo motor 9, the servo motor 9 is installed on the tank body 8, the output end of the servo motor 9 is connected with a screw rod 10, the screw rod 10 transversely extends into the tank body 8, a moving part 11 is in threaded connection with the screw rod 10, and stirring blades 12 and a cleaning plate 13 are installed on the moving part 11; the servo motor 9 is started to drive the screw rod 10 to rotate, so that the moving part 11 is driven to move back and forth along the arrangement direction of the screw rod 10, and the stirring blades 12 are used for stirring the culture medium; a storage cylinder is arranged on the cleaning plate 13, a plurality of movable scrapers 14 are mounted in the storage cylinder, and springs 15 are connected between any movable scraper 14 and the bottom wall of the storage cylinder; one end of the movable scraper 14 close to the inner wall of the can body 8 is provided with a wiping block 19 made of flexible material; since the can body 8 is not in a regular cylindrical shape and the inner diameter thereof gradually decreases from the middle to both sides, a plurality of movable scrapers 14 and wiping blocks 19 made of flexible material are provided to make the portion for scraping the inner wall of the can body 8 to be adapted to the shape of the can body 8 as much as possible, so as to obtain a better scraping effect.
Further, the sterilizer 1 comprises a vacuum port 16, a steam inlet 17 and an opening 18 for the entry of the culture medium; the sterilizer 1 is connected with the air extractor through the vacuumizing port 16 before high-temperature sterilization, and after the sterilizer 1 is vacuumized, steam is injected into the sterilizer 1 through the steam inlet 17 for high-temperature sterilization.
Further, an inoculation spray head 4 for spraying strains into the cooling inoculation machine 3 is arranged on the cooling inoculation machine 3; after the temperature of the culture medium in the cooling inoculation machine 3 is reduced to the inoculation temperature, the strains are injected into the cooling inoculation machine 3 through the inoculation nozzle 4 for inoculation.
Further, the temperature cooling mechanism 6 comprises a cooling interlayer sleeved on the cooling inoculation machine 3 and a vacuum quick cooling system connected with the cooling inoculation machine 3 through a heat exchanger; the top of the cooling interlayer is provided with a cooling water inlet, and the bottom of the cooling interlayer is provided with a cooling water outlet.
Furthermore, temperature sensors for detecting the temperature of the strains and the temperature of the culture medium are arranged in the sterilizer 1 and the cooling inoculator 3.
Further, two ends of the tank body 8 are provided with bearings, and the screw rod 10 is connected with the tank body 8 through the bearings.
The utility model discloses a concrete theory of operation:
(1) the operator adds the culture medium to the sterilizer 1 through the inlet and preheats the sterilizer 1 with steam through the steam inlet 17.
(2) The interior of the tank body 8 of the sterilizer 1 is vacuumized through a vacuumizing port 16, and after vacuumizing, steam is introduced into the tank body 8 through a steam inlet 17 to heat and sterilize the culture medium.
(3) The stirring device 7 is started, the servo motor 9 is started to drive the screw rod 10 to rotate, so that the moving part 11 is driven to move back and forth along the arrangement direction of the screw rod 10, the movable scraper 14 and the wiping block 19 can rotate back and forth to scrape the inner wall of the tank body 8 for cleaning, and the stirring blades 12 uniformly stir the culture medium, thereby facilitating subsequent inoculation.
(4) After the sterilization is finished, a feed valve is opened to convey the culture medium in the sterilizer 1 into the cooling inoculator 3; after the feeding is finished, the feeding valve is closed, the temperature cooling mechanism 6 is opened, the culture medium in the cooling inoculation machine 3 is rapidly cooled, and the temperature of the culture medium is reduced to the inoculation temperature.
(5) The strains are sprayed into the cooling inoculation machine 3 through the inoculation spray head 4, meanwhile, the stirring device 7 in the cooling inoculation machine 3 is used for stirring and inoculating, and the wiping block 19 scrapes off the culture medium attached to the inner wall of the tank body 8; and finally, opening a discharge valve to pour the inoculated mixture out of the feed opening 5.
It will be understood that modifications and variations can be made by persons skilled in the art in light of the above teachings and all such modifications and variations are considered to be within the scope of the invention as defined by the following claims.
Claims (6)
1. A culture medium inoculation mixing device comprises a sterilizer for sterilizing a culture medium, wherein the sterilizer is connected with a cooling inoculation machine through a feed inlet which is controlled to be opened and closed by a feed valve, and the cooling inoculation machine is provided with a feed outlet which is controlled to be opened and closed by a discharge valve; the cooling inoculation machine is provided with a temperature cooling mechanism for reducing the temperature in the cooling inoculation machine to the inoculation temperature; the culture medium sterilization device is characterized in that the sterilization machine and the cooling inoculation machine respectively comprise a cylindrical tank body, the inner diameter of the tank body is gradually reduced from the middle part to two sides, and the feed inlet and the feed outlet are respectively positioned at the upper part and the lower part of the middle part of the tank body; the stirring device comprises a servo motor, the servo motor is arranged on the tank body, the output end of the servo motor is connected with a screw rod, the screw rod transversely extends into the tank body, a moving part is in threaded connection with the screw rod, and stirring blades and a cleaning plate are arranged on the moving part; a containing cylinder is arranged on the cleaning plate, a plurality of movable scrapers are mounted in the containing cylinder, and springs are connected between any movable scraper and the bottom wall of the containing cylinder; one end of the movable scraper close to the inner wall of the tank body is provided with a wiping block made of flexible materials.
2. A media inoculation mixing apparatus according to claim 1, wherein the steriliser includes a vacuum port, a steam inlet and an opening for the ingress of media.
3. The culture medium inoculation mixing device of claim 1, wherein the cooling inoculator is provided with an inoculation spray head for spraying the inoculum into the cooling inoculator.
4. The culture medium inoculation mixing device of claim 1, wherein the temperature cooling mechanism comprises a cooling interlayer sleeved on the cooling inoculation machine and a vacuum rapid cooling system connected with the cooling inoculation machine through a heat exchanger.
5. The culture medium inoculation mixing device of claim 1, wherein the sterilizer and the cooling inoculator are both provided with temperature sensors for detecting the temperature of strains and culture media.
6. A culture medium inoculation and mixing device according to claim 1, wherein bearings are arranged at two ends of the tank body, and the screw rod is connected with the tank body through the bearings.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120188627.XU CN214546164U (en) | 2021-01-22 | 2021-01-22 | Culture medium inoculation mixing arrangement |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120188627.XU CN214546164U (en) | 2021-01-22 | 2021-01-22 | Culture medium inoculation mixing arrangement |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214546164U true CN214546164U (en) | 2021-11-02 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120188627.XU Active CN214546164U (en) | 2021-01-22 | 2021-01-22 | Culture medium inoculation mixing arrangement |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214546164U (en) |
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2021
- 2021-01-22 CN CN202120188627.XU patent/CN214546164U/en active Active
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