CN214529066U - Static tensile intervention device of cell culture - Google Patents
Static tensile intervention device of cell culture Download PDFInfo
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- CN214529066U CN214529066U CN202023078219.3U CN202023078219U CN214529066U CN 214529066 U CN214529066 U CN 214529066U CN 202023078219 U CN202023078219 U CN 202023078219U CN 214529066 U CN214529066 U CN 214529066U
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Abstract
The utility model relates to a static tensile intervention device of cell culture, including base, support frame and cell culture board, be equipped with a plurality of fore-set on the base, the support frame is including setting up the vertical support frame and the setting of both sides are in around the base the horizontal support frame of the base left and right sides, be equipped with on the cell culture board a plurality of with the culture hole that the fore-set corresponds, be equipped with the elasticity basement membrane in the culture hole, cell culture board detachably sets up on the support frame, the top of fore-set with the bottom of elasticity basement membrane bonds. The utility model is used for the cell in the cell experiment is tensile, the tensile degree of regulation cell that can be convenient, easy operation, the reliability is high.
Description
The technical field is as follows:
the utility model relates to a biological assay apparatus technical field, concretely relates to static tensile intervention device of cell culture.
Background art:
in current medical biology research, the culture of cells is often static, and the cells are seeded in hard plastic multi-well plates. In this case, it is difficult to artificially stretch the cells. In the actual physiological environment, cells of the organism can be stretched along with the movement of the organism, and in order to enable the cell culture environment to be closer to the actual physiological environment, the influence of the stretching of the organism on the cells is considered, and the cells need to be stretched in the cell culture process by adopting a corresponding means, so that the accuracy of a cell experiment is improved. Patent No. ZL201920833355.7 cultivates the board through setting up, cultivate lid and base, it fixes in the base top to cultivate board detachably, form an airtight space between cultivation board and the base, it places in the cultivation board top to cultivate the lid, be equipped with the several on the cultivation board and cultivate the hole, it is equipped with compound elastic basement membrane to cultivate the hole bottom, the outside fixedly connected with aspiration pump of base and air intake pump, aspiration pump and air intake pump communicate airtight space respectively, aspiration pump and air intake pump electricity respectively are connected with the controller, the accessible aspiration pump, the drawing/compression function to the cell is realized in the cooperation of air intake pump and automatically controlled pneumatic valve. However, in this device, the cell culture plate needs to be placed in a closed environment, and the basement membrane is stretched by the air suction pump and the air inlet pump, which has high requirements on the reliability of the device, and the stretching degree of the cells cannot be accurately controlled.
The utility model has the following contents:
technical problem to be solved
The utility model aims at providing a static tensile intervention device of cell culture for cell in the cell culture experiment is tensile, and it is higher to the equipment reliability requirement to solve prior art, and can not the problem of the tensile degree of accurate control cell.
The technical scheme is as follows:
in order to solve the technical problem, the utility model adopts the following technical scheme: the utility model provides a static tensile intervention device of cell culture, includes base, support frame and cell culture board, be equipped with a plurality of fore-set on the base, the support frame is including setting up the vertical support frame of both sides is in with the setting around the base the horizontal support frame of the base left and right sides, be equipped with on the cell culture board a plurality of with the cultivation hole that the fore-set corresponds, be equipped with the elasticity basement membrane in the cultivation hole, cell culture board detachably sets up on the support frame, the top of fore-set with the bottom of elasticity basement membrane bonds.
The cell culture plate is used for carrying out cell culture, and the cell sample that needs to cultivate is placed in the culture hole, the top of elasticity basement membrane, the cell culture plate passes through vertical support frame with horizontal support frame carries out horizontal direction's spacing, the cell culture plate can pass through manual regulation along the support frame reciprocates, the fore-set will the elasticity basement membrane struts, thereby realizes the drawing of the cell of elasticity basement membrane top, through control the fore-set will the height that the elasticity basement membrane propped up can control the tensile degree of cell.
Further, the top pillar comprises a fixed pillar and a lifting pillar, a sliding groove is formed in the fixed pillar, the bottom end of the lifting pillar is arranged in the sliding groove, a through groove is formed in the side wall of the fixed pillar, a threaded rod penetrating through the through groove is formed in the side surface of the lifting pillar, a locking nut is connected to the threaded rod in a threaded mode, placing platforms are arranged on the longitudinal supporting frame and the transverse supporting frame, a threaded hole is formed in the side surface of the longitudinal supporting frame, a locking screw is arranged in the threaded hole, the lifting pillar can lift along the sliding groove in the fixed pillar, the height of the top pillar is changed, the threaded rod can slide in the through groove, the lifting of the lifting pillar is controlled, the height of the top pillar is convenient to adjust, the stretching degree of cells is changed, and the locking nut is used for propping against the outer wall of the fixed pillar through rotation after the adjustment is completed, so as to lock the position of the lifting column, the placing table is used for placing the cell culture plate, and the locking screw is used for locking the cell culture plate to prevent movement.
Further, be equipped with a plurality of backing plates on the base, be equipped with on the backing plate with the perforation that the fore-set corresponds, every the thickness of backing plate is all different, the cell culture board is placed the top of backing plate, the top of cell culture board is equipped with compresses tightly the crossbeam, be equipped with the shifting chute on the horizontal support frame, the both ends that compress tightly the crossbeam insert in the shifting chute, the backing plate is used for the pad the below of cell culture board, the fore-set passes on the backing plate the perforation is placed through with different thickness the backing plate is placed the below of cell culture board, can adjust the fore-set inserts cultivate downthehole length to realize the regulation of cell tensile degree.
Furthermore, the side of the lifting column and the side of the fixing column are provided with scales, and the scales are convenient for experimenters to control the height of the lifting column so as to accurately control the opening height of the elastic base membrane.
Further, the diameter of the top column is 18mm, the diameter of the culture hole is 37mm, the thickness of the cushion plate is 1-4.75mm, and the adjustment of 10% -40% of cell stretching rate can be realized.
Furthermore, the total number of the top columns is six, the cell culture plate is a six-hole plate, and the six-hole plate is a common cell culture device, is easy to obtain and is convenient to use.
(III) the beneficial effects are as follows:
compared with the prior art, the utility model discloses the beneficial effect who produces is: through setting up base and support frame, the cell culture board sets up between the support frame and can height-adjusting, sets up the fore-set that corresponds with the culture hole on the cell culture board on the base, sets up the elasticity basement membrane in the culture hole, and through adjusting the height that the fore-set struts the elasticity basement membrane struts, the tensile degree of cell is adjusted on the realization elasticity basement membrane, and the reliability is high, easy operation.
Description of the drawings:
in order to more clearly illustrate the technical solution in the embodiments of the present invention, the drawings required to be used in the embodiments will be briefly described below.
Fig. 1 is a schematic structural diagram of the base and the supporting frame of the cell culture static stretching intervention device according to an embodiment of the present invention;
FIG. 2 is a side view of a cell culture static tension intervention device according to a first embodiment of the present invention;
FIG. 3 is a cross-sectional view of a cell culture static tensile intervention device according to a first embodiment of the present invention;
FIG. 4 is a longitudinal cross-sectional view of a cell culture static tensile intervention device according to a first embodiment of the present invention;
FIG. 5 is a top view of a cell culture static tension intervention device according to a first embodiment of the present invention;
fig. 6 is a schematic structural diagram of the base and the supporting frame of the cell culture static stretching intervention device according to the second embodiment of the present invention;
FIG. 7 is a cross-sectional view of a cell culture static tension intervention device according to example two of the present invention;
FIG. 8 is a schematic structural diagram of the backing plate of the cell culture static tension intervention device according to the second embodiment of the present invention;
FIG. 9 is a top view of a cell culture static tension intervention device according to example two of the present invention;
in the figure: 1. a base; 2. a support frame; 21. a longitudinal support frame; 22. a transverse support frame; 3. a cell culture plate; 4. a top pillar; 41. fixing a column; 42. a lifting column; 5. a culture well; 6. an elastic base film; 7. a chute; 8. penetrating a groove; 9. a threaded rod; 10. a lock nut; 11. a placing table; 12. a threaded hole; 13. locking the screw; 14. a base plate; 15. perforating; 16. compressing the beam; 17. a moving groove; 18. scale division
The specific implementation mode is as follows:
the technical solution in the embodiments of the present invention will be clearly and completely described below with reference to the accompanying drawings in the embodiments of the present invention.
Example one
As shown in fig. 1, fig. 2, fig. 3, the static tensile device of interveneeing of cell culture of fig. 4 and fig. 5, including base 1, support frame 2 and cell culture board 3, be equipped with a plurality of fore-set 4 on the base 1, support frame 2 is including setting up vertical support frame 21 and the horizontal support frame 22 of setting in the base 1 left and right sides in base 1 front and back both sides, be equipped with the culture hole 5 that a plurality of and fore-set 4 correspond on the cell culture board 3, be equipped with elasticity basement membrane 6 in the culture hole 5, cell culture board 3 detachably sets up on support frame 21, the top of fore-set 4 bonds with the bottom of elasticity basement membrane 6.
Preferably, the top pillar 4 comprises a fixed pillar 41 and a lifting pillar 42, a sliding slot 7 is arranged in the fixed pillar 41, the bottom end of the lifting pillar 42 is arranged in the sliding slot 7, a through slot 8 is arranged on the side wall of the fixed pillar 41, a threaded rod 9 penetrating through the through slot 8 is arranged on the side surface of the lifting pillar 42, a locking nut 10 is connected to the threaded rod 9 in a threaded manner, placing tables 11 are arranged on the longitudinal supporting frame 21 and the transverse supporting frame 22, a threaded hole 12 is arranged on the side surface of the longitudinal supporting frame 21, a locking screw 13 is arranged in the threaded hole 12, the lifting pillar 42 can lift along the sliding slot 7 in the fixed pillar 41 so as to change the height of the top pillar 4, the threaded rod 9 can slide in the through slot 8 so as to control the lifting of the lifting pillar 42, the height of the top pillar 4 can be adjusted conveniently so as to change the stretching degree of cells, the locking nut 10 is used for propping against the outer wall of the fixed pillar 41 through rotation after the adjustment is completed so as to lock the position of the lifting pillar 42, the placing table 11 is used for placing the cell culture plate 3, and the locking screw 13 is used for locking the cell culture plate 3 to prevent movement.
Preferably, the lifting column 42 and the fixed column 41 are provided with scales 18 on the side surfaces, and the scales 18 facilitate the experimenter to control the height of the lifting column 42 so as to accurately control the distraction height of the elastic basement membrane 6.
Preferably, the total number of the top pillars 4 is six, the cell culture plate 3 is a six-hole plate, and the six-hole plate is a common cell culture device, is easy to obtain and is convenient to use.
The utility model discloses embodiment one the application method of static tensile intervention device of cell culture as follows:
place cell culture plate 3 on placing platform 11, the position of cultivation hole 5 is adjusted well with fore-set 4, the top of lift post 42 bonds with the bottom of the interior elasticity basement membrane 6 of cultivation hole 5, through screwing in locking screw 13 to screw hole 12 and locking cell culture plate 3, put into the cell that needs to cultivate on the elasticity basement membrane 6 and cultivate, when needing to carry out the cell drawing, loosen lock nut 10, pulling lift post 42 slides from top to bottom along spout 7, with the height of adjusting lift post 42, thereby stretch elasticity basement membrane 6, observe the height of rising through scale 18, with cell regulation to suitable tensile rate, it can to adjust the position fixation of post 42 through screwing up lock nut 10 after finishing.
Example two
As shown in fig. 6, fig. 7, fig. 8 and fig. 9, including base 1, support frame 2 and cell culture plate 3, be equipped with a plurality of fore-set 4 on base 1, support frame 2 is including setting up vertical support frame 21 and the horizontal support frame 22 of setting in the base 1 left and right sides in base 1 front and back both sides, be equipped with the culture hole 5 that a plurality of and fore-set 4 correspond on the cell culture plate 3, be equipped with elasticity basement membrane 6 in the culture hole 5, cell culture plate 3 detachably sets up on support frame 21, the top of fore-set 4 bonds with the bottom of elasticity basement membrane 6.
Preferably, be equipped with a plurality of backing plates 14 on the base 1, be equipped with the perforation 15 that corresponds with the fore-set 4 on the backing plate 14, the thickness of every backing plate 14 is all different, cell culture plate 3 places the top at backing plate 14, the top of cell culture plate 3 is equipped with compresses tightly crossbeam 16, be equipped with the shifting chute 17 on the horizontal support frame 22, compress tightly the both ends of crossbeam 16 and insert in the shifting chute 17, backing plate 14 is used for filling up the below at cell culture plate 3, the fore-set 4 passes perforation 15 on backing plate 14, through placing the backing plate 14 of different thickness in the below of cell culture plate 3, can adjust the fore-set 4 length of inserting in cultivateing the hole 5, thereby realize the regulation of cell tensile degree.
Preferably, the diameter of the top pillar 4 is 18mm, the diameter of the culture hole 5 is 37mm, and the thickness of the backing plate 14 is 1-4.75mm, so that the adjustment of 10% -40% cell elongation can be realized.
Preferably, the total number of the top pillars 4 is six, the cell culture plate 3 is a six-hole plate, and the six-hole plate is a common cell culture device, is easy to obtain and is convenient to use.
The embodiment of the utility model provides a use method of static tensile intervention device of cell culture as follows:
place cell culture plate 3 between vertical support frame 21 and horizontal support frame 22, the position of culture hole 5 is adjusted well with the apical pillar 4, the difference of the stretch rate of adjusting as required, put into the backing plate 14 of different thickness below cell culture plate 3, place cell culture plate 3 on backing plate 14 again, apical pillar 4 passes perforation 15 on backing plate 14, with the jack-up of elasticity basement membrane 6 in the culture hole 5, thereby stretch the cell of cultivateing on the elasticity basement membrane 6, realize the accurate control of cell stretch rate.
To sum up, the utility model provides a static tensile intervention device of cell culture for cell in the cell culture experiment is tensile, and it is higher to the equipment reliability requirement to solve prior art, and can not the problem of the tensile degree of accurate control cell.
The present invention has been described above by way of example, but the present invention is not limited to the above-mentioned embodiments, and any modification or variation based on the present invention is within the scope of the present invention.
Claims (6)
1. A cell culture static stretching intervenes device which characterized in that: including base, support frame and cell culture board, be equipped with a plurality of fore-set on the base, the support frame is including setting up longitudinal support frame and the setting of both sides are in around the base the horizontal support frame of the base left and right sides, be equipped with on the cell culture board a plurality of with the culture hole that the fore-set corresponds, be equipped with the elasticity basement membrane in the culture hole, cell culture board detachably sets up on the support frame, the top of fore-set with the bottom of elasticity basement membrane bonds.
2. The cell culture static tension intervention device of claim 1, wherein: the top column comprises a fixing column and a lifting column, a sliding groove is formed in the fixing column, the bottom end of the lifting column is arranged in the sliding groove, a through groove is formed in the side wall of the fixing column, a threaded rod penetrating through the through groove is arranged on the side face of the lifting column, a locking nut is connected to the threaded rod in a threaded mode, placing platforms are arranged on the longitudinal supporting frame and the transverse supporting frame, a threaded hole is formed in the side face of the longitudinal supporting frame, and a locking screw is arranged in the threaded hole.
3. The cell culture static tension intervention device of claim 1, wherein: the cell culture plate is characterized in that a plurality of base plates are arranged on the base, through holes corresponding to the jacking columns are formed in the base plates, the thickness of each base plate is different, the cell culture plate is placed above the base plates, a pressing cross beam is arranged above the cell culture plate, a moving groove is formed in the transverse supporting frame, and two ends of the pressing cross beam are inserted into the moving grooves.
4. The cell culture static tension intervention device of claim 2, wherein: the side surfaces of the lifting column and the fixing column are provided with scales.
5. The cell culture static tension intervention device of claim 3, wherein: the diameter of the top column is 18mm, the diameter of the culture hole is 37mm, and the thickness of the cushion plate is 1-4.75 mm.
6. The cell culture static tension intervention device of claim 1, wherein: six top columns are provided, and the cell culture plate is a six-hole plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023078219.3U CN214529066U (en) | 2020-12-20 | 2020-12-20 | Static tensile intervention device of cell culture |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202023078219.3U CN214529066U (en) | 2020-12-20 | 2020-12-20 | Static tensile intervention device of cell culture |
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| Publication Number | Publication Date |
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| CN214529066U true CN214529066U (en) | 2021-10-29 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202023078219.3U Active CN214529066U (en) | 2020-12-20 | 2020-12-20 | Static tensile intervention device of cell culture |
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| CN (1) | CN214529066U (en) |
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