CN214473401U - Interleukin 33 inspection kit - Google Patents
Interleukin 33 inspection kit Download PDFInfo
- Publication number
- CN214473401U CN214473401U CN202120096705.3U CN202120096705U CN214473401U CN 214473401 U CN214473401 U CN 214473401U CN 202120096705 U CN202120096705 U CN 202120096705U CN 214473401 U CN214473401 U CN 214473401U
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- China
- Prior art keywords
- reagent storage
- reagent
- storage cavity
- enzyme
- storage chamber
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- Expired - Fee Related
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- 108010067003 Interleukin-33 Proteins 0.000 title claims abstract description 18
- 102000017761 Interleukin-33 Human genes 0.000 title claims abstract description 18
- 238000007689 inspection Methods 0.000 title claims abstract description 12
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 96
- 102000004190 Enzymes Human genes 0.000 claims abstract description 26
- 108090000790 Enzymes Proteins 0.000 claims abstract description 26
- 238000000034 method Methods 0.000 claims description 5
- 230000008569 process Effects 0.000 claims description 5
- 239000004033 plastic Substances 0.000 claims description 3
- 229920003023 plastic Polymers 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 2
- 239000012528 membrane Substances 0.000 claims 1
- 238000005259 measurement Methods 0.000 abstract description 7
- 238000007789 sealing Methods 0.000 abstract description 7
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 230000009286 beneficial effect Effects 0.000 description 4
- 102000004127 Cytokines Human genes 0.000 description 3
- 108090000695 Cytokines Proteins 0.000 description 3
- 238000010586 diagram Methods 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 2
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000001105 regulatory effect Effects 0.000 description 2
- 230000003844 B-cell-activation Effects 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102000039996 IL-1 family Human genes 0.000 description 1
- 108091069196 IL-1 family Proteins 0.000 description 1
- 102000015696 Interleukins Human genes 0.000 description 1
- 108010063738 Interleukins Proteins 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 210000004556 brain Anatomy 0.000 description 1
- 230000024245 cell differentiation Effects 0.000 description 1
- 210000003855 cell nucleus Anatomy 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000009977 dual effect Effects 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 210000002865 immune cell Anatomy 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000028709 inflammatory response Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 239000002985 plastic film Substances 0.000 description 1
- 229920006255 plastic film Polymers 0.000 description 1
- 229920000915 polyvinyl chloride Polymers 0.000 description 1
- 239000004800 polyvinyl chloride Substances 0.000 description 1
- 230000000770 proinflammatory effect Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 210000003491 skin Anatomy 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 210000002784 stomach Anatomy 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 230000029069 type 2 immune response Effects 0.000 description 1
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- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The interleukin 33 inspection kit comprises a box-shaped body, wherein the box-shaped body comprises a box body and an upper cover, a reagent storage cavity and a sealing film for sealing the reagent storage cavity are arranged on the lower end surface of the upper cover, the box body consists of a reagent storage cavity puncture device and an enzyme-labeled coated plate mounting frame, the reagent storage cavity puncture device is positioned below the reagent storage cavity and corresponds to the reagent storage cavity, the enzyme-labeled coated plate mounting frame is positioned below the reagent storage cavity puncture device, and the reagent storage cavity puncture device is used for puncturing the reagent storage cavity and guiding a reagent in the reagent storage cavity to an enzyme-labeled coated plate positioned in the enzyme-labeled coated plate mounting frame; set up reagent and store the chamber, can store the quantitative reagent in reagent storage intracavity at the production stage, stop the artifical reagent measurement that adds of dependence in experimental stage, reduce reagent metering error, through piercing the device with the leading-in ELIAS mark peridium board of reagent on, whole all can go on under high airtight state, avoid the pollution that probably appears.
Description
Technical Field
The utility model relates to an interleukin 33 inspection kit.
Background
The interleukin is a cytokine which is produced by various cells and acts on various cells, plays an important role in transmitting information, activating and regulating immune cells, mediating T, B cell activation, proliferation and differentiation and in inflammatory response, the interleukin 33(IL-33) is a cytokine of IL-1 family, is widely distributed and highly expressed in stomach, lung, spinal cord, brain and skin, has dual functions of IL-33, can participate in regulating Th2 immune response as a secretory cytokine and has the function of a pro-inflammatory factor, and can be positioned in a cell nucleus as a nuclear factor to play a role in transcription inhibition.
For the measurement of the level of interleukin 33(IL-33), various measurement modes of a kit exist in the prior art, the modes are low in cost and simple, but it should be noted that the measurement of interleukin 33 requires the addition of various reagents and has a strict process sequence, but the existing kit needs to manually measure the quantity of the reagents during the addition of the reagents, so that errors are serious, and the existing kit has no indication on the process sequence and has no correction effect on inexperienced personnel or negligence of personnel.
Disclosure of Invention
For solving the defect that prior art exists, the utility model provides a but the strict control reagent measurement to can have the kit of suggestive to operation technology.
In order to achieve the above purpose, the utility model adopts the following technical scheme: the interleukin 33 inspection kit comprises a box-shaped body, wherein the box-shaped body comprises a box body and an upper cover, a reagent storage cavity and a sealing film for sealing the reagent storage cavity are arranged on the lower end surface of the upper cover, the box body consists of a reagent storage cavity puncture device and an enzyme-labeled coated plate mounting frame, the reagent storage cavity puncture device is positioned below the reagent storage cavity and corresponds to the reagent storage cavity, the enzyme-labeled coated plate mounting frame is positioned below the reagent storage cavity puncture device, and the reagent storage cavity puncture device is used for puncturing the reagent storage cavity and guiding a reagent in the reagent storage cavity to an enzyme-labeled coated plate positioned in the enzyme-labeled coated plate mounting frame; the beneficial effects of this technical scheme lie in being provided with reagent and storing the chamber, consequently can store the quantitative reagent in reagent storage intracavity at the production stage to stop the artifical reagent measurement that adds of dependence of experimental stage, reduce reagent measurement error, and the pollution circumstances that probably appears still can effectively be avoided to this technical characteristic, through pricking the device with the leading-in enzyme-labeled peridium board of reagent, whole journey all can go on under highly airtight state.
Furthermore, the reagent storage cavities and the reagent storage cavity puncturing devices corresponding to the reagent storage cavities are multiple groups and are arranged in sequence, and reagents required by corresponding processes are stored in the corresponding reagent storage cavities; the beneficial effects of this technical scheme lie in: the reagent storage cavities are arranged in sequence, the puncture devices are also used for sequentially removing lightning, different practices can be injected into the reagent storage cavities in the corresponding sequence according to different operation sequences in the production stage of the device, and due to the fact that the reagent can be discharged through the puncture devices, in the practical stage, an operator can judge whether the previous step is carried out or not by judging whether the reagent storage cavities in the previous sequence are punctured or whether the puncture devices are in a proceeding state or not, so that sequence errors caused by manual errors or insufficient experience are avoided, and the experimental correctness is ensured.
Further, the reagent storage cavity puncturing device comprises a through hole and a rotating shaft rotating in the through hole, a puncturing needle is fixedly installed at the side end of the rotating shaft in the through hole, the puncturing needle rotates in the through hole through the rotating shaft to enable the puncturing needle to be in a horizontal state or a vertical state, and the puncturing needle is used for puncturing the reagent storage cavity and guiding a reagent in the reagent storage cavity into the enzyme-labeled coated plate; the beneficial effects of this technical scheme lie in: the puncture needle is installed in the side of pivot, when consequently carrying out the water conservancy diversion to the reagent of reagent storage intracavity, can be very big avoid the pivot to the horizontal drainage of reagent, make the reagent effectively lead to on the enzyme-labeled peridium board under the action of gravity.
Furthermore, a rotating handle is mounted at the end part of the rotating shaft, the rotating handle is positioned at the side end of the box body, a rotating limiting step is arranged at the side end of the rotating handle, and the rotating limiting step limits the rotating angle of the rotating handle; the beneficial effects of this technical scheme lie in: because the limiting step is arranged, the rotating handle can be limited when the rotating handle rotates to drive the rotating shaft to rotate to enable the puncture needle to be in a vertical state, the puncture needle is prevented from being in the vertical state, and adverse effects on flow guiding are avoided.
Furthermore, the puncture needle, the rotating shaft and the rotating handle are made of plastic materials; the technical scheme can obviously reduce the manufacturing and using cost of the disposable appliance.
Further, an inner groove is formed in the bottom end of the enzyme-labeled coating plate mounting rack; the technical scheme can increase the taking-out convenience of the enzyme-labeled coated plate.
Drawings
For further explanation of the present invention, the drawings and embodiments are listed below.
Fig. 1 is a schematic structural diagram of the present invention.
FIG. 2 is a schematic structural view of the utility model when no ELISA coated plate is placed.
Fig. 3 is the enlarged structure schematic diagram of the reagent storage chamber puncture device of the utility model.
Fig. 4 is a schematic diagram of the structure of the enzyme-labeled coating plate of the present invention.
Detailed Description
As shown in FIGS. 1 to 4, an interleukin 33 inspection kit comprises a box-shaped body, the box-shaped body comprises a box body 1 and an upper cover 2, a reagent storage cavity 21 and a sealing film (not shown) for sealing the reagent storage cavity 21 are arranged on the lower end surface of the upper cover 2, the sealing film adopts a film-shaped body disclosed in the prior art, for example, a medical grade polyvinyl chloride plastic film, the box body 1 consists of a reagent storage cavity puncture device 3 and an enzyme-labeled coated plate mounting rack 4, the reagent storage cavity puncture device 3 is positioned below a reagent storage cavity 21, and corresponds to the reagent storage cavity 21, the enzyme-labeled coated plate mounting rack 4 is positioned below the reagent storage cavity puncturing device 3, the reagent storage cavity puncturing device 3 is used for puncturing the reagent storage cavity 21, and the reagent in the reagent storage cavity 21 is guided to the enzyme-labeled coated plate 5 in the enzyme-labeled coated plate mounting rack 4.
Furthermore, the reagent storage cavities 21 and the corresponding reagent storage cavity puncturing devices 3 are in multiple groups and are arranged in sequence, and reagents required by corresponding processes are stored in the corresponding reagent storage cavities 21.
Further, the reagent storage cavity puncturing device 3 comprises a through hole 31 and a rotating shaft 32 rotating in the through hole 31, a puncturing needle 33 is fixedly installed at the side end of the rotating shaft 32 in the through hole 31, the puncturing needle 33 rotates in the through hole 31 through the rotating shaft 32, the puncturing needle 33 is in a horizontal state or a vertical state, and the puncturing needle 33 is used for puncturing the reagent storage cavity 21 and guiding the reagent in the reagent storage cavity 21 onto the enzyme-labeled coated plate 5.
Further, the end of the rotating shaft 32 is provided with a rotating handle 34, the rotating handle 34 is located at the side end of the box body 1, the side end of the rotating handle 34 is provided with a rotating limiting step 35, and the rotating limiting step 35 limits the rotating angle of the rotating handle 34.
Further, the puncture needle 33, the rotating shaft 34 and the rotating handle 35 are made of plastic materials.
Further, the bottom end of the enzyme-labeled coated plate mounting rack 4 is provided with an inner groove 41.
Obviously, the above embodiment is only one example of the present invention, and any simple improvement in the structure or principle provided by the present invention is within the protection scope of the present invention.
Claims (6)
1. The utility model provides an interleukin 33 inspection kit, includes the box-shaped body, its characterized in that the box-shaped body includes box body and upper cover, be provided with the seal membrane that chamber and sealed reagent storage chamber were stored to the reagent on the terminal surface under the upper cover, the box body comprises reagent storage chamber puncture device and enzyme-labeled peridium board mounting bracket, reagent storage chamber puncture device is located reagent storage chamber below to it is corresponding with reagent storage chamber, enzyme-labeled peridium board mounting bracket is located reagent storage chamber puncture device below, reagent storage chamber puncture device is used for puncturing reagent storage chamber to the reagent is stored the reagent with reagent storage intracavity reagent water conservancy diversion to be located the enzyme-labeled peridium board in the enzyme-labeled peridium board mounting bracket.
2. The interleukin 33 inspection kit according to claim 1, wherein the reagent storage chambers and the corresponding reagent storage chamber puncturing devices are arranged in a plurality of groups, and are sequentially arranged, and the reagents required by the corresponding processes are stored in the corresponding reagent storage chambers.
3. The interleukin 33 inspection kit according to claim 1 or 2, wherein the reagent storage chamber puncturing device comprises a through hole and a rotating shaft rotating in the through hole, a puncturing needle is fixedly mounted at a side end of the rotating shaft in the through hole, the puncturing needle rotates in the through hole through the rotating shaft to enable the puncturing needle to be in a horizontal state or a vertical state, and the puncturing needle is used for puncturing the reagent storage chamber and guiding the reagent in the reagent storage chamber onto the enzyme-labeled coated plate.
4. The interleukin 33 inspection kit according to claim 3, wherein a rotating handle is mounted at an end of the rotating shaft, the rotating handle is located at a side end of the box body, and a rotation limiting step is provided at a side end of the rotating handle, and the rotation limiting step limits a rotation angle of the rotating handle.
5. The interleukin 33 inspection kit according to claim 3, wherein the pricking pin, the rotating shaft and the rotating handle are made of plastic.
6. The interleukin 33 inspection kit according to claim 1, wherein an inner groove is formed at the bottom end of the enzyme-labeled coated plate mounting rack.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120096705.3U CN214473401U (en) | 2021-01-14 | 2021-01-14 | Interleukin 33 inspection kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202120096705.3U CN214473401U (en) | 2021-01-14 | 2021-01-14 | Interleukin 33 inspection kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN214473401U true CN214473401U (en) | 2021-10-22 |
Family
ID=78111831
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202120096705.3U Expired - Fee Related CN214473401U (en) | 2021-01-14 | 2021-01-14 | Interleukin 33 inspection kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN214473401U (en) |
-
2021
- 2021-01-14 CN CN202120096705.3U patent/CN214473401U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20211022 |