CN214327752U

CN214327752U - High-efficient hypoxemia cell culture device

Info

Publication number: CN214327752U
Application number: CN202022332015.1U
Authority: CN
Inventors: 杨琨; 陈伟基; 丁海娥; 李铀; 冯哲; 卢利平; 魏锁成
Original assignee: Northwest Minzu University
Current assignee: Northwest Minzu University
Priority date: 2020-10-19
Filing date: 2020-10-19
Publication date: 2021-10-01
Anticipated expiration: 2030-10-19

Abstract

The utility model relates to a cell culture device, concretely relates to high-efficient hypoxemia cell culture device. The problems of high difficulty in regulating and controlling the hypoxia condition and long time consumption of the conventional cell culture device are effectively solved; including the cell culture unit, install the nitrogen gas unit on the cell culture unit, the nitrogen gas blast pipe and the air intake pipe of nitrogen gas unit all communicate with the gas processing unit, and nitrogen gas and air after the gas processing unit is handled pass through the connecting pipe and get into the cell culture unit, install the exhaust seat on the cell culture unit. This device is through adopting nitrogen gas generation and air fusion heating back, according to oxygen concentration requirement injection gas, can reach low oxygen or anaerobic environment by the high efficiency.

Description

High-efficient hypoxemia cell culture device

Technical Field

The utility model relates to a cell culture device, concretely relates to high-efficient hypoxemia cell culture device.

Background

Changes in oxygen content are one of the most important regulators throughout the life cycle, extending from embryonic development, maintenance of normal body function, to pathological processes such as disease and aging, with hypoxia being the most common basic environment. The state of culture when the oxygen in the cell or organ is lower than the oxygen level in the air is called hypoxic culture.

In the experimental research of culturing cells under an anoxic state, how to rapidly place the cells in an hypoxic environment is a problem troubling researchers, and the automatic balance between a hypoxic gas phase and a liquid phase environment in which the cells are placed and a gas environment in which the cells are replaced by the hypoxic gas is long in time consumption. The conventional cell hypoxia culture equipment is mainly a three-gas incubator and has the problem of high price, and the time for balancing the hypoxia concentration in the incubator to the required concentration after the door of the incubator is opened and closed every time is up to several hours, so that the experimental condition is not favorable for accurate regulation and control, and the time is longer for completely lacking oxygen.

SUMMERY OF THE UTILITY MODEL

The utility model provides a high-efficient hypoxia cell culture device, the effectual current cell culture device hypoxemia condition of having solved regulation and control degree of difficulty is big and the problem of consuming time length, and this device is through adopting nitrogen gas generation and air fusion heating back, requires to inject into gas according to oxygen concentration, can high efficiency reach hypoxemia or anaerobic environment.

In order to achieve the above object, the utility model adopts the following technical scheme:

the utility model provides a high-efficient hypoxic cell culture device, includes cell culture unit 1, its characterized in that: the cell culture unit 1 is provided with a nitrogen unit 2, a nitrogen exhaust pipe 12 and an air inlet pipe 14 of the nitrogen unit 2 are communicated with the gas processing unit 3, nitrogen and air treated by the gas processing unit 3 enter the cell culture unit 1 through a connecting pipe 19, and the cell culture unit 1 is provided with an exhaust seat 4.

The cell culture unit 1 comprises a culture box body 5, a sealing door 6, a vessel placing plate 7 and an oxygen sensor 8, wherein the openable sealing door 6 is arranged on the culture box body 5, and the vessel placing plate 7 and the oxygen sensor 8 are arranged in the culture box body 5.

The nitrogen unit 2 comprises a triangular box 9 and a nitrogen bottle 10, nitrogen in the nitrogen bottle 10 is discharged through a nitrogen exhaust pipe 12, and the nitrogen exhaust pipe 12 is provided with a nitrogen valve.

The gas processing unit 3 comprises a gas processing box 14, a heating pipe 15 and a double-spiral pipe 16, the heating pipe 15 and the double-spiral pipe 16 are installed in the gas processing box 14, one end of the double-spiral pipe 16 is connected with a nitrogen exhaust pipe 12 and an air intake pipe 14, and the other end of the double-spiral pipe is communicated with a culture box body 5 of the cell culture unit 1 through a connecting pipe 19.

The double spiral pipe 16 is composed of two sets of spiral pipes, including a nitrogen spiral pipe 1601 and an air spiral pipe 1602, the nitrogen spiral pipe 1601 is communicated with the nitrogen exhaust pipe 12, and the air spiral pipe 1602 is connected with the air inlet pipe 14.

The air inlet pipe 14 is provided with an air valve 15.

An air main valve 20 is provided on the connection pipe 19.

The exhaust seat 4 comprises a micro air pump 21 and an exhaust pipe 22, the micro air pump 21 is installed on the incubator body 5, and the air in the incubator body 5 is exhausted through the exhaust pipe 22.

The cell culture unit 1 is provided with a controller 23, and the controller 23 is in circuit connection with the heating pipe 15 and the oxygen sensor 8.

The utility model has the advantages that: 1) this device passes through nitrogen gas unit discharge nitrogen gas rapidly, carries out temperature adjustment through gas processing unit, simultaneously with air treatment, input nitrogen gas or the mist of nitrogen gas and air that can be rapid.

2) The device can rapidly reach a low-oxygen or anaerobic environment according to the requirement of culture conditions, and has high control efficiency and high operation reliability.

This device is through adopting nitrogen gas generation and air fusion heating back, according to oxygen concentration requirement injection gas, can reach low oxygen or anaerobic environment by the high efficiency.

Drawings

FIG. 1 is a first schematic structural diagram of the present invention;

FIG. 2 is a second schematic structural view of the present invention;

FIG. 3 is a schematic view of the internal installation structure of the incubator body;

FIG. 4 is a schematic structural view of a nitrogen unit;

FIG. 5 is a schematic view of a gas processing cell configuration;

FIG. 6 is a schematic view of a double helix construction;

shown in the figure: the device comprises a cell culture unit 1, a nitrogen unit 2, a gas processing unit 3, an exhaust seat 4, a culture box body 5, a sealing door 6, a vessel placing plate 7, an oxygen sensor 8, a triangular box 9, a nitrogen bottle 10, a nitrogen exhaust pipe 12, a nitrogen gas valve 13, a gas processing box 14, a heating pipe 15, a double spiral pipe 16, a nitrogen spiral pipe 1601, an air spiral pipe 1602, an air inlet pipe 17, an air gas valve 18, a connecting pipe 19, an air main valve 20, a micro air pump 21, an exhaust pipe 22 and a controller 23.

Detailed Description

For making the utility model discloses a utility model purpose, technical scheme, beneficial effect are clearer, combine the figure in the following to explain in detail the embodiment of the utility model.

Example 1

The utility model provides a high-efficient hypoxic cell culture device aims at providing the high-efficient supply of nitrogen gas for the culture unit can be in hypoxemia or anaerobic state rapidly, and the temperature of steerable air supply and nitrogen gas supply simultaneously, concrete structure is as follows.

The device is divided into four unit structures, namely a cell culture unit 1, a nitrogen unit 2, a gas processing unit 3 and an exhaust seat 4. The cell culture unit 1 is used for placing a cell culture dish and is filled with hypoxia or anaerobism in the cell culture unit 1. The nitrogen unit 2 is used for chemically generating nitrogen gas, filtering the nitrogen gas and discharging the nitrogen gas. The gas processing unit 3 is used to adjust the temperature of air or nitrogen gas injected into the cell culture unit 1. The exhaust seat 4 is used to exhaust the gas in the cell culture unit 1.

The cell culture unit 1 is shown in FIGS. 1, 2 and 3. The culture box body 5 is used as a carrier, the seal door 6 is arranged on the culture box body 5, and after the seal door 6 is closed, the interior of the culture box body 5 can be in a sealed state. A dish placing tray 7 and an oxygen sensor 8 are provided inside the incubator body 5. The dish placing tray 7 is used for placing cell culture dishes. The oxygen sensor 8 detects the oxygen concentration inside the incubator body 5 and displays it by the controller 23 attached to the sealing door 6.

The nitrogen unit structure is shown in fig. 1, 2 and 4, and a triangular box 9 is used as a carrier. A nitrogen cylinder 10 is installed inside the triangular box 9. The nitrogen in the nitrogen cylinder 10 is discharged through the nitrogen exhaust pipe. A nitrogen gas valve 13 is installed in the nitrogen gas exhaust pipe 12.

The gas processing unit 3 is shown in fig. 1, 2 and 5 for temperature regulation of air and generated nitrogen gas. The gas processing unit 3 uses a gas processing box 14 as a carrier, and a plurality of electric heating pipes 15 are installed on the gas processing box 14. Double coils 16 are installed in the plurality of heating pipes 15. The double spiral tube 16 is composed of a nitrogen spiral tube 1601 and an air spiral tube 1602 as shown in FIG. 6, wherein the upper end of the nitrogen spiral tube 1601 is connected with the nitrogen exhaust pipe 12, and the lower end is connected with the incubator 5 through a connecting pipe 19. The upper end of the air spiral pipe 1602 is connected to an air inlet pipe 17, and the lower end is communicated with the incubator 5 through a connecting pipe 19.

An air valve 18 is provided in the air intake pipe 17 to control the amount of air intake.

A gas main valve 20 is provided in the connection pipe 18.

The exhaust seat 4 is used for exhausting the gas in the culture box body 5, and consists of a micro air pump 21 and an exhaust pipe 22, and the gas is exhausted from the exhaust pipe 22 through the action of the micro air pump 21.

The nitrogen can be replaced by carbon dioxide to realize the injection of different gases. The injected nitrogen and carbon dioxide are sterilized by the filter element.

It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.

Furthermore, it should be understood that although the present description refers to embodiments, not every embodiment may contain only a single embodiment, and such description is for clarity only, and those skilled in the art should integrate the description, and the embodiments may be combined as appropriate to form other embodiments understood by those skilled in the art.

Claims

1. The utility model provides a high-efficient hypoxic cell culture device, includes cell culture unit, its characterized in that: install the nitrogen gas unit on the cell culture unit, nitrogen gas blast pipe and the air intake pipe of nitrogen gas unit all communicate with gas processing unit, and nitrogen gas and air after gas processing unit handles pass through the connecting pipe and get into the cell culture unit, install the exhaust seat on the cell culture unit.

2. A hypoxic cell culture apparatus according to claim 1, wherein: the cell culture unit comprises a culture box body, a sealing door, a vessel placing plate and an oxygen sensor, wherein the openable sealing door is arranged on the culture box body, and the vessel placing plate and the oxygen sensor are arranged in the culture box body.

3. A hypoxic cell culture apparatus according to claim 1, wherein: the nitrogen unit comprises a triangular box and a nitrogen bottle, nitrogen in the nitrogen bottle is discharged through a nitrogen exhaust pipe, and a nitrogen valve is arranged on the nitrogen exhaust pipe.

4. A hypoxic cell culture apparatus according to claim 1, wherein: the gas processing unit comprises a gas processing box, a heating pipe and a double-spiral pipe, the heating pipe and the double-spiral pipe are installed in the gas processing box, one end of the double-spiral pipe is connected with a nitrogen exhaust pipe and an air intake pipe, and the other end of the double-spiral pipe is communicated with a culture box body of the cell culture unit through a connecting pipe.

5. A hypoxic cell culture apparatus according to claim 4, wherein: the double spiral pipe comprises two sets of spiral pipelines, including nitrogen gas spiral pipe and air spiral pipe, nitrogen gas spiral pipe and nitrogen gas blast pipe intercommunication, air spiral pipe and air intake pipe connection.

6. A hypoxic cell culture apparatus according to claim 1 or claim 5, wherein: the air inlet pipe is provided with an air valve.

7. A hypoxic cell culture apparatus according to claim 1 or claim 4, wherein: and an air main valve is arranged on the connecting pipe.

8. A hypoxic cell culture apparatus according to claim 1, wherein: the exhaust seat comprises a micro air pump and an exhaust pipe, the micro air pump is installed on the culture box body, and gas in the culture box body is exhausted through the exhaust pipe.

9. A hypoxic cell culture apparatus according to claim 1, wherein: and the cell culture unit is provided with a controller, and the controller is in circuit connection with the heating pipe and the oxygen sensor.