CN214325745U

CN214325745U - Equipment is deposited to seamless cloning kit

Info

Publication number: CN214325745U
Application number: CN202120202368.1U
Authority: CN
Inventors: 高素枝
Original assignee: Beijing Kangrun Chengye Biotechnology Co ltd
Current assignee: Beijing Kangrun Chengye Biotechnology Co ltd
Priority date: 2021-01-22
Filing date: 2021-01-22
Publication date: 2021-10-01
Anticipated expiration: 2031-01-22

Abstract

The utility model relates to a seamless cloning technology field just discloses a seamless cloning kit deposits equipment, including depositing the platform, the top fixedly connected with of depositing the platform deposits the case, deposit the inner wall fixedly connected with sealing layer of case, the fixed slot has been seted up in the front of depositing the case, the inner wall fixedly connected with fixed bolster of fixed slot, the inner wall swing joint of fixed slot has the diaphragm, the draw-in groove has been seted up in the front of diaphragm. This equipment is deposited to seamless cloning kit has reached the purpose that this equipment is deposited to seamless cloning kit high efficiency used to solved the unable high efficiency used problem of equipment is deposited to general seamless cloning kit, the production of kit just can break away from tedious reagent preparation and optimization process in order to make the experimenter, the high efficiency is used and can be reduced the loaded down with trivial details configuration of user of service, can be high-efficient concentrated deposit of carrying out reagent collect and use, it is convenient to have brought for daily seamless cloning, has made things convenient for the use.

Description

Equipment is deposited to seamless cloning kit

Technical Field

The utility model relates to a seamless cloning technology field specifically is a seamless cloning kit deposits equipment.

Background

Seamless cloning is different from traditional PCR product cloning, the only difference is that the tail end of a vector and the tail end of a primer are provided with 15-20 homologous bases, the two ends of the obtained PCR product are respectively provided with 15-20 bases homologous with a vector sequence, complementary pairing and cyclization are carried out by depending on the acting force between the bases, the obtained PCR product can be directly used for transforming host bacteria without enzyme connection, the linear plasmid ring entering the host bacteria can repair a gap by depending on a self enzyme system, the seamless cloning and assembly technology is a novel, rapid and simple cloning method and aims to overcome the defects The seamless cloning of the boxes for chemical reagents such as drug residues, virus types and the like requires the storage of a kit.

However, the problem that the use efficiency is low mostly exists in the equipment is deposited to general seamless cloning kit on the market, deposits the reagent pipe in the reagent box, and the reagent box needs carry out the access of reagent pipe according to the demand of using at any time, and this just needs the reagent box can high-efficiently provide and deposits for the reagent pipe, and the reagent box that can not high-efficiently use can influence the use, can't cooperate medical personnel's demand operation.

SUMMERY OF THE UTILITY MODEL

Technical problem to be solved

The not enough to prior art, the utility model provides a seamless cloning kit deposits equipment possesses the height and inhales advantages such as use, has solved the problem that equipment availability factor is low is deposited to general seamless cloning kit.

(II) technical scheme

For the purpose of realizing that the above-mentioned seamless cloning kit deposits the high-efficient use of equipment, the utility model provides a following technical scheme: the utility model provides a seamless cloning kit deposits equipment, is including depositing the platform, the top fixedly connected with of depositing the platform deposits the case, deposit the inner wall fixedly connected with sealing layer of case, deposit the front of case and seted up the fixed slot, the inner wall fixedly connected with fixed bolster of fixed slot, the inner wall swing joint of fixed slot has the diaphragm, the draw-in groove has been seted up in the front of diaphragm.

The inner wall swing joint of draw-in groove has the reagent pipe, deposit the interior roof fixedly connected with bactericidal lamp of case, the mounting groove has been seted up in the front of depositing the case, the inner wall swing joint of mounting groove has infrared lamp, the front swing joint of depositing the case has the chamber door, the front fixedly connected with keeper of chamber door.

Preferably, the shape and size of the fixing groove are matched with those of the transverse plate, and the storage box is movably connected with the transverse plate through the fixing groove.

Preferably, the shape and the size of the sealing layer are matched with those of the storage box, and the sealing layer is a rubber sealing layer.

Preferably, the shape and size of the clamping groove are matched with those of the reagent tube, and the transverse plate is movably connected with the reagent tube through the clamping groove.

Preferably, the front surface of the storage box is fixedly connected with a hinge, and the storage box is movably connected with the box door through the hinge.

Preferably, the number of the hinges is four, every two hinges form a group, and the two groups of hinges are symmetrically arranged by taking the perpendicular bisector of the box door as a symmetry axis.

Preferably, the number of the germicidal lamps is two, and the two germicidal lamps are symmetrically arranged by taking the perpendicular bisector of the storage box as the symmetry axis.

(III) advantageous effects

Compared with the prior art, the utility model provides a seamless cloning kit deposits equipment possesses following beneficial effect:

1. this equipment is deposited to seamless cloning kit, through depositing the case, the fixed slot, the fixed bolster, the diaphragm, the draw-in groove, the reagent pipe, the bactericidal lamp, the hinge, mutually supporting of chamber door and keeper is used, the purpose that equipment high efficiency was used is deposited to this seamless cloning kit has been reached, thereby the problem of the unable high-efficient use of equipment is deposited to general seamless cloning kit is solved, the production of kit is exactly in order to make the experimenter can break away from tedious reagent and prepare and the optimization process, the loaded down with trivial details configuration of user of can reducing the high-efficient use, can be high-efficient concentrated deposit of carrying out reagent collect and use, it is convenient to have brought for daily seamless cloning, and convenient use is provided.

2. This equipment is deposited to seamless cloning kit, through depositing the platform, deposit mutually supporting of case and sealing layer and use, reached this and deposited the sealed effectual purpose of case, deposit the case in the in-process of using, need open according to the demand of using and deposit and take out, the sealing layer can make the sealed of box better, can block that external dust etc. gets into by the gap and deposit the inside environment of case influence.

3. This equipment is deposited to seamless cloning kit uses through depositing mutually supporting of case, sealing layer, fixed slot, fixed bolster and diaphragm, has reached this diaphragm and has dismantled convenient purpose, and the diaphragm needs some dismantlements disinfection timely when depositing reagent pipe, and the diaphragm that conveniently dismantles has satisfied seamless cloning to the sterile daily operation of the internals of depositing the case, and simple operation is worth promoting.

Drawings

FIG. 1 is a schematic structural view of the present invention;

fig. 2 is a front view of the present invention;

FIG. 3 is an enlarged view of the structure A of FIG. 1 according to the present invention;

fig. 4 is an enlarged view of the structure B in fig. 1 according to the present invention.

In the figure: 1. a storage table; 2. a storage box; 3. a sealing layer; 4. fixing grooves; 5. a fixing pad; 6. a transverse plate; 7. a card slot; 8. a reagent tube; 9. a germicidal lamp; 10. mounting grooves; 11. an infrared lamp; 12. a hinge; 13. a box door; 14. a door bolt.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to fig. 1-4, a seamless cloning kit storage device comprises a storage table 1, a storage box 2 is fixedly connected to the top of the storage table 1, a sealing layer 3 is fixedly connected to the inner wall of the storage box 2, the shape and size of the sealing layer 3 are matched with those of the storage box 2, the sealing layer 3 is a rubber sealing layer, rubber is an elastic polymer and can be obtained from sap of some plants or artificial, according to different manufacturing modes, the rubber can be divided into synthetic rubber and natural rubber, a fixing groove 4 is formed in the front of the storage box 2, a fixing pad 5 is fixedly connected to the inner wall of the fixing groove 4, a transverse plate 6 is movably connected to the inner wall of the fixing groove 4, a clamping groove 7 is formed in the front of the transverse plate 6, the shape and size of the fixing groove 4 are matched with those of the transverse plate 6, and the storage box 2 is movably connected with the transverse plate 6 through the fixing groove 4, the rethread is deposited platform 1, is deposited case 2 and sealing layer 3 and is used mutually supporting, has reached this sealed effectual purpose of depositing case 2, deposits case 2 in the in-process of using, needs open according to the demand of using and deposits and take out, and sealing layer 3 can make the sealed of box better, can block that external dust etc. gets into by the gap and deposit case 2 influence inside environment.

The inner wall of the clamping groove 7 is movably connected with a reagent tube 8, the shape and the size of the clamping groove 7 are matched with those of the reagent tube 8, the transverse plate 6 is movably connected with the reagent tube 8 through the clamping groove 7, the inner top wall of the storage box 2 is fixedly connected with two bactericidal lamps 9, the two bactericidal lamps 9 are symmetrically arranged by taking the perpendicular bisector of the storage box 2 as a symmetry axis, the front side of the storage box 2 is provided with a mounting groove 10, the inner wall of the mounting groove 10 is movably connected with an infrared lamp 11, the infrared heating industrial lamp is made of blown bubble shells or pressed glass and is a reflector lamp which can provide energy radiation capable of being accurately controlled, the front side of the storage box 2 is fixedly connected with hinges 12, the storage box 2 is movably connected with a box door 13 through hinges 12, the number of the hinges 12 is four, every two hinges 12 are in one group, and the two groups of hinges 12 are symmetrically arranged by taking the perpendicular bisector of the box door 13 as a symmetry axis, the front of the storage box 2 is movably connected with a box door 13, the storage box 2 and a sealing layer 3 are mutually matched for use through the storage platform 1, the storage box 2 and the sealing layer 3, the purpose of good sealing effect of the storage box 2 is achieved, the storage box 2 needs to be opened for storage and taken out according to the use requirement in the use process, the sealing layer 3 can enable the box body to be better sealed, external dust and the like can be prevented from entering the storage box 2 from gaps to influence the internal environment, the front of the box door 13 is fixedly connected with a bolt 14, and the seamless cloning kit storage equipment is efficiently used through the mutual matching of the storage box 2, a fixed groove 4, a fixed cushion 5, a transverse plate 6, a clamping groove 7, a reagent tube 8, a sterilizing lamp 9, a hinge 12, the box door 13 and the bolt 14, so that the problem that the common seamless cloning kit storage equipment cannot be efficiently used is solved, and the production of the kit is just for enabling an experimenter to be capable of getting rid of heavy reagent preparation and optimization processes, the high-efficient use can reduce the loaded down with trivial details configuration of user of service personnel, can be high-efficient concentrated carry out depositing of reagent collect and use, and it is convenient to have brought for daily seamless cloning, has made things convenient for the use.

The electrical components presented in the document are all electrically connected with an external master controller and 220V mains, and the master controller can be a conventional known device controlled by a computer or the like.

When the reagent tube storage box is used, the reagent tubes 8 need to be stored, the box door 13 is opened through the door bolt 14, the reagent tubes 8 are placed into the clamping grooves 7 in the storage box 2 to be fixed, then the box door 13 is closed, and the sterilizing lamp 9 and the infrared lamp 11 sterilize and disinfect the reagent tubes 8 inside, so that the operation is completed.

To sum up, this equipment is deposited to seamless cloning kit has reached the purpose that this equipment is deposited to seamless cloning kit high-efficient use, and the production of kit is exactly in order to make the experimenter can get rid of tedious reagent and prepare and optimization process, and the loaded down with trivial details configuration of user of high-efficient use can be reduced, can high-efficient concentrated deposit of carrying on reagent collect and use, has brought conveniently for daily seamless cloning.

It is noted that, herein, relational terms such as first and second, and the like may be used solely to distinguish one entity or action from another entity or action without necessarily requiring or implying any actual such relationship or order between such entities or actions. Also, the terms "comprises," "comprising," or any other variation thereof, are intended to cover a non-exclusive inclusion, such that a process, method, article, or apparatus that comprises a list of elements does not include only those elements but may include other elements not expressly listed or inherent to such process, method, article, or apparatus.

Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.

Claims

1. A seamless cloning kit storage device comprising a storage station (1), characterized in that: the top of the storage table (1) is fixedly connected with a storage box (2), the inner wall of the storage box (2) is fixedly connected with a sealing layer (3), the front side of the storage box (2) is provided with a fixed groove (4), the inner wall of the fixed groove (4) is fixedly connected with a fixed pad (5), the inner wall of the fixed groove (4) is movably connected with a transverse plate (6), and the front side of the transverse plate (6) is provided with a clamping groove (7);

the inner wall swing joint of draw-in groove (7) has reagent pipe (8), deposit interior roof fixedly connected with bactericidal lamp (9) of case (2), deposit the front of case (2) and seted up mounting groove (10), the inner wall swing joint of mounting groove (10) has infrared lamp (11), the front swing joint of depositing case (2) has chamber door (13), the front fixedly connected with keeper (14) of chamber door (13).

2. The seamless cloning kit storage device of claim 1, wherein: the shape and size of the fixing groove (4) and the shape and size of the transverse plate (6) are matched with each other, and the storage box (2) is movably connected with the transverse plate (6) through the fixing groove (4).

3. The seamless cloning kit storage device of claim 1, wherein: the shape and the size of the sealing layer (3) are matched with those of the storage box (2), and the sealing layer (3) is a rubber sealing layer.

4. The seamless cloning kit storage device of claim 1, wherein: the shape and size of the clamping groove (7) are matched with those of the reagent tube (8), and the transverse plate (6) is movably connected with the reagent tube (8) through the clamping groove (7).

5. The seamless cloning kit storage device of claim 1, wherein: the front surface of the storage box (2) is fixedly connected with a hinge (12), and the storage box (2) is movably connected with a box door (13) through the hinge (12).

6. The seamless cloning kit storage device of claim 5, wherein: the number of the hinges (12) is four, every two hinges (12) are in one group, and the two groups of hinges (12) are symmetrically arranged by taking the perpendicular bisector of the box door (13) as a symmetry axis.

7. The seamless cloning kit storage device of claim 1, wherein: the quantity of bactericidal lamp (9) is two, and two bactericidal lamps (9) are symmetry axis symmetry setting with the perpendicular bisector of depositing case (2).