CN214216627U - Freezing storage tube for storing tissue samples - Google Patents

Abstract

The utility model discloses a freezing storage tube of storage tissue sample, including body and tube cap, the tube cap top is equipped with first through-hole, have in the body and prolong two at most tissue samples that its length direction set up according to the preface and place the chamber, adjacent two tissue samples are placed and are equipped with the baffle between the chamber, all be provided with a plurality of second through-holes on the baffle, tissue samples are placed chamber one side and all are equipped with the opening, the lateral wall is equipped with the lateral wall lid that is connected with the body that corresponds with open position outward. The utility model provides a can store up freezing storage pipe of organizing has solved the problem that the security is poor, storage space utilization is low.

Description

Freezing storage tube for storing tissue samples

Technical Field

The utility model relates to a field is stored in the tissue refrigeration particularly, relates to a can store freezing storage pipe of tissue sample.

Background

In the laboratory, when animal experiments are performed, such as harvesting mouse tissue, there are many cases (e.g., for analysis of DNA/RNA or protein levels) where cryogenic storage is required to ensure the integrity of tissue components. Microcentrifuge tubes (EP tubes) are common tools for sample handling or storage. Although EP tubes have many advantages, such as: the oblique angle design of the edge of the pipe cover has good touch and is easy to open; the original semi-closing function reduces the pollution caused by multiple sample adding operations; a high-quality die is adopted, the inner wall is ultra-smooth, and the liquid adsorption residue is reduced; the high-quality polypropylene material is adopted, so that the paint is non-toxic, the pipe wall is ultra-clear, and the paint is easy to observe.

However, there are many disadvantages in the process of storing mouse tissues. Before mouse tissue collection, the required number of EP tubes needs to be prepared, and information of a clear sample is marked on each tube, so that the time is consumed, and the information marked by the marker pen is easily damaged in a low-temperature environment or in the presence of chemicals. And (3) putting the organized EP tube into a small liquid nitrogen box in the collection process, and uniformly transferring the organized EP tube into a square box for preservation in a refrigerator at-80 ℃ after the tissue collection is finished. Because each EP tube can only store one tissue sample, the space utilization rate of the refrigerator at minus 80 ℃ is extremely low; sometimes an organization needs to be stored in several pieces, not only requiring a large number of EP tubes, but also being time consuming to pick up and register. When samples are processed, such as RNA/protein level analysis, organized EP tubes are taken out of a refrigerator at-80 ℃ and placed in a small liquid nitrogen box for individual processing, but the EP tubes for storage are often taken out of the liquid nitrogen box because of poor sealing and too fast liquid transformation, so that the EP tubes explode, and the safety is poor, and the samples are lost.

Therefore, the freezing storage tube for storing the frozen tissues is high in safety, low in cost and convenient and fast in information marking, saves time and storage space to a great extent, and has great popularization and application values.

Disclosure of Invention

An object of the utility model is to overcome not enough among the prior art, provide a but freezing storage pipe of storage refrigeration tissue, solved the problem that the security is poor, storage space utilization is low.

The utility model provides a following technical scheme:

a freezing storage tube for storing tissue samples comprises a tube body, a tube cover and a partition plate arranged in the tube body, wherein a first through hole is formed above the tube cover, two to more tissue sample placing cavities are formed in the tube body and are sequentially arranged along the length direction of the tube body, the tissue sample placing cavities are separated by the partition plate, a plurality of second through holes are formed in the partition plate, openings are formed in one sides of the tissue sample placing cavities, side wall covers which correspond to the openings in position and are connected with the tube body are arranged outside the side walls, and the openings of the tissue sample placing cavities can be sealed by the side wall covers;

further, the pipe cover and the pipe body are integrally connected, the side wall cover is provided with a first through hole, the first through hole above the pipe cover and the first through hole on the side wall cover are both provided with through hole plugs corresponding to the through holes, and the through hole plugs are both provided with pull rings;

preferably, the outer side wall of the pipe body is provided with a label pasting part with a non-smooth surface, so that low-temperature label paper can be pasted and fixed conveniently;

preferably, the outer side wall of the tissue sample placing cavity is provided with corresponding serial numbers and/or label pasting positions;

preferably, a pipe body buckle is arranged in the pipe body, a groove corresponding to the pipe body buckle is formed in the partition plate, and the partition plate can be connected with the pipe body through the buckle;

preferably, the tube body is a cylinder or a cuboid and is made of transparent materials;

preferably, the pipe body is made of polypropylene materials, and the volume of the pipe body is 2-5 ml;

compared with the prior art, the beneficial effects of the utility model are that:

(1) the utility model discloses a through-hole has all been designed to tube cap, lateral wall lid and intermediate bottom of freezing storage pipe, because intraductal liquid-gas transformation is too fast when avoiding freezing storage pipe to take out from the liquid nitrogen, and pressure can not bear and explode to the pipe wall greatly. On one hand, the safety is improved, and on the other hand, the sample loss caused by explosion is avoided;

(2) the utility model discloses a freezing storage intraductal baffle that is equipped with of pipe divide into a plurality of sample storage units with freezing storage pipe, and a freezing storage pipe can save many samples, very big has made things convenient for depositing of different tissue or the same tissue, and each layer is equipped with solitary lateral wall lid in addition, makes things convenient for each sample to put into alone and take out. On the one hand, the storage space of the sample is greatly saved, on the other hand, the utilization rate of the freezing storage tube is improved, and the cost is saved.

In addition, the position of pasting label paper is left to the body outer wall, and label printer is equipped with the low temperature label, directly print out paste label paper the position can, on the one hand save time, on the other hand avoid the information of marker pen mark to receive the damage that low temperature environment or chemical pollution took place.

Drawings

FIG. 1 is a schematic view of the freezing storage tube of the present invention;

FIG. 2 is a schematic view of the construction of the sidewall cover;

fig. 3 is a schematic view of the structure of the separator.

1. The tube cap, 2, the body, 3, the chamber is placed to the tissue sample, 4, the opening, 5, the first through-hole is covered to the tube cap, 6, the lateral wall lid, 7, the first through-hole is covered to the lateral wall, 8, the pull ring, 9, label paste the department, 10, the serial number, 11, first through-hole stopper, 12, through-hole stopper pull ring, 13, baffle, 14, the second through-hole.

Detailed Description

The present invention will be further described with reference to the accompanying drawings. The following examples are only for illustrating the technical solutions of the present invention more clearly, and the protection scope of the present invention is not limited thereby.

It should be noted that, in the description of the present invention, the terms "front", "rear", "left", "right", "upper", "lower", "inner", "outer", etc. indicate the directions or positional relationships based on the directions or positional relationships shown in the drawings, and are only for convenience of description of the present invention but do not require the present invention to be constructed and operated in a specific direction, and thus, should not be construed as limiting the present invention. As used in the description of the present invention, the terms "front," "back," "left," "right," "up," "down" and "in" refer to directions in the drawings, and the terms "inner" and "outer" refer to directions toward and away from, respectively, the geometric center of a particular component.

As shown in fig. 1, a freezing storage tube for storing tissue samples comprises a tube body 2 and a tube cover 1 integrally connected with the tube body 2, wherein a first through hole 5 and a pull ring 8 are arranged above the tube cover 1, 4 tissue sample placing cavities 3 are arranged in the tube body 2, an opening 4 is arranged on one side of each tissue sample placing cavity, a side wall cover 6 connected with the tube body is arranged on the outer side wall of the tissue sample placing cavity 3 corresponding to the opening 4, and a first through hole 7 is arranged on the side wall cover 6; the outer side wall of the tissue sample placing cavity 3 is provided with a corresponding tissue sample placing cavity number 10, and a corresponding label pasting part 9 is arranged, wherein the label pasting part 9 is a non-smooth surface. 4 samples can be stored simultaneously in the freezing storage tube, the utilization rate of the storage tube is improved, and the time for taking and registering is saved.

As shown in fig. 2, a first through hole 7 is formed in the side wall cover 6, the first through hole 7 is provided with a corresponding first through hole plug 11, and a through hole plug pull ring 12 is arranged on the first through hole plug 11; the first through hole and the first through hole plug above the tube cover 1 are the same as the structure shown in fig. 2.

As shown in FIG. 3, the tissue sample placing chamber is partitioned by a partition plate 13, and the partition plate 13 is provided with a plurality of second through holes 14.

The storage mode of the freezing storage pipe that this embodiment provided does: can be independently and horizontally or vertically stored in a low-temperature refrigerator, or can be intensively stored in a box matched with the freezing storage tube and then is placed in a low-temperature environment, such as a liquid nitrogen tank.

The use mode of the freezing storage tube provided by the embodiment is as follows:

1. storage of the same organization

The liver and lung tissues of mice have multiple pages, and when used for protein level analysis, the same site is generally required to be compared between the experimental group and the control group. The freezing storage tube designed by the invention is very convenient and fast. Taking lung as an example, four lung lobes of the upper left, lower left, upper right and lower right of the experimental group and the control group of mice are collected. Before collection, the required number of the freezing storage tubes is prepared and is respectively used for a control group and an experimental group. Design label (including mouse group, age, tissue-here specific lung lobes, time of collection). The label printer prints the low temperature label and attaches it to the side wall label of freezing storage tube and pastes department 9. The 4 side wall caps 6 are opened and the through-hole plugs of the tube caps 1 are pulled off. Four upper left, lower left, upper right and lower right lung lobes of the mouse are collected, the corresponding tissue sample placing cavity 3 is placed from the side wall tube opening 4, the side wall cover 6 is closed, and the freezing storage tube is rapidly placed into the prepared liquid nitrogen box. And after tissue collection of all mice is finished, transferring all the freezing storage tubes into a square box, and storing in a refrigerator at-80 ℃. When the sample is analyzed, a refrigerator at-80 ℃ takes out the storage tube where the required sample is positioned, and the storage tube is placed in a liquid nitrogen box. The storage tube through hole plug 11 is pulled out, and the intermediate partition plate 13 is provided with a plurality of through holes 14, so that the storage tube cannot explode, and samples can be processed one by one with confidence. The remaining tissue can be placed in a-80 ℃ freezer for further preservation.

2. Storage of different tissues

Different tissues of the same mouse were collected, such as heart, pancreas, kidney (two on the left and right). Preparation before collection is as above. The heart, pancreas, right kidney, left kidney of the mice were collected. The corresponding tissue sample application chamber 3 is placed through the sidewall tube opening 4, the sidewall cover 6 is closed, and the cryostorage tube is quickly placed into the prepared liquid nitrogen cassette. The following operation is the same as above.

The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, a plurality of modifications and variations can be made without departing from the technical principle of the present invention, and these modifications and variations should also be considered as the protection scope of the present invention.

Claims (6)

1. The utility model provides a freezing storage tube of storage tissue sample, includes body and tube cap, its characterized in that, the tube cap top is equipped with first through-hole, have in the body and place the chamber along its length direction two at most tissue samples that set up according to the preface, adjacent two tissue samples are placed and are equipped with the baffle between the chamber, all be provided with a plurality of second through-holes on the baffle, tissue samples place chamber one side and all are equipped with the opening, the lateral wall is equipped with the lateral wall lid that is connected with the body that corresponds with open position outward.

2. The chilled storage tube of claim 1, wherein the sidewall cover has a first through hole therein.

3. The chilled storage tube of claim 1 or 2, wherein the first throughbore is provided with a vent plug, each vent plug provided with a pull ring.

4. The cryogenic storage tube of claim 1, wherein the tissue sample placement chamber outer sidewalls are each provided with a corresponding number and/or label sticker.

5. The cryogenic storage tube of claim 1, wherein the tube body is cylindrical or rectangular.

6. The chilled storage tube of claim 1, wherein the tube body is made of polypropylene and has a volume of 2-5 ml.

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