CN214142379U

CN214142379U - Nucleic acid fluorescence detection equipment

Info

Publication number: CN214142379U
Application number: CN202022766519.4U
Authority: CN
Inventors: 王敏
Original assignee: Shanghai Shanxiang Biotechnology Co ltd
Current assignee: Shanghai Shanxiang Biotechnology Co ltd
Priority date: 2020-11-26
Filing date: 2020-11-26
Publication date: 2021-09-07
Anticipated expiration: 2030-11-26

Abstract

The utility model discloses a nucleic acid fluorescence check out test set, which comprises a housin, the inner wall fixedly connected with hot plate of casing, the lower extreme inner wall of casing is provided with and holds the ware, the chamber is placed to the inner wall fixedly connected with that holds the ware, the inner wall fixedly connected with motor of placing the chamber, the one end extension of motor is provided with the pivot, the pivot is run through and is placed chamber fixedly connected with stirring piece, the upper surface of casing is provided with the recess, the lateral wall fixedly connected with fixed plate of recess, one side fixedly connected with connecting rod of fixed plate, the outer wall of connecting rod rotates and is connected with the handle, the lower fixed surface of casing is connected with the connecting cylinder, the inner wall fixedly connected with second electric putter of connecting cylinder. The utility model discloses in, for nucleic acid provides suitable living environment and nucleic acid and fluorescence mark mix fully, can improve the accuracy that detects, check out test set still portable in addition, the suitability is strong.

Description

Nucleic acid fluorescence detection equipment

Technical Field

The utility model relates to a check out test set field especially relates to a nucleic acid fluorescence check out test set.

Background

Nucleic acid is a general term of deoxyribonucleic acid (DNA) and ribonucleic acid (RNA), is a biological macromolecular compound formed by polymerizing a plurality of nucleotide monomers, is one of the most basic substances of life, is a biopolymer, is an essential constituent substance of all known life forms, is the most important substance of all biomolecules, and is widely present in all animal and plant cells and microorganisms.

At present, excessive fluorescent dye is added for nucleic acid detection, the fluorescent dye emits a fluorescent signal after being specifically doped into a nucleic acid double strand, dye molecules which are not doped into the strand cannot emit any fluorescent signal, then the nucleic acid is detected by observing the quantity of the fluorescent light, the activity of the nucleic acid can be influenced by the external temperature, so that the mixing condition of the nucleic acid and the fluorescent light is influenced, the accuracy of the nucleic acid fluorescent detection is further influenced, in addition, whether the nucleic acid and the fluorescent mark are fully mixed or not can also influence the accuracy of the nucleic acid fluorescent detection, and finally, the nucleic acid to be detected is delivered to detection equipment by the existing detection equipment through extraction, so that the detection equipment is very inconvenient to move, and certain limitation is brought to the detection.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing a nucleic acid fluorescence detection device.

In order to achieve the above purpose, the utility model adopts the following technical scheme: a nucleic acid fluorescence detection device comprises a shell, wherein a heating plate is fixedly connected to the inner wall of the shell, a containing vessel is arranged on the inner wall of the lower end of the shell, a containing cavity is fixedly connected to the inner wall of the containing vessel, a motor is fixedly connected to the inner wall of the containing cavity, a rotating shaft is arranged at one end of the motor in an extending mode and penetrates through the containing cavity to be fixedly connected with a stirring sheet;

the upper surface of casing is provided with the recess, the lateral wall fixedly connected with fixed plate of recess, one side fixedly connected with connecting rod of fixed plate, the outer wall of connecting rod rotates and is connected with the handle, the lower fixed surface of casing is connected with the connecting cylinder, the inner wall fixedly connected with second electric putter of connecting cylinder, second electric putter's one end fixedly connected with connecting plate, the lower fixed surface of connecting plate is connected with the bracing piece, the one end fixedly connected with guide arm of bracing piece, the outer wall of guide arm rotates and is connected with the pulley.

As a further description of the above technical solution:

the upper end inner wall of casing is provided with thermal-insulated chamber, the inner wall fixedly connected with electric putter in thermal-insulated chamber, electric putter runs through thermal-insulated chamber fixedly connected with test probe.

As a further description of the above technical solution:

the upper end inner wall of casing just is located the right side in thermal-insulated chamber and is provided with the fixed block, the lower surface of fixed block is provided with temperature-sensing ware.

As a further description of the above technical solution:

the surface of the shell is rotatably connected with two groups of shell doors through hinges, and handles are arranged on the surfaces of the shell doors.

And a projection screen is arranged on the surface of one group of the shell doors, and an operation panel is arranged on the surface of one group of the shell doors.

As a further description of the above technical solution:

the surface of operation panel is provided with the display screen, the surface of operation panel just is located the below of display screen and is provided with the operating key.

As a further description of the above technical solution:

and a switch is arranged on the surface of the operating panel and below the operating key.

As a further description of the above technical solution:

the switch is electrically connected with the temperature sensor, the first electric push rod, the second electric push rod and the motor.

The utility model discloses following beneficial effect has:

1. the utility model discloses in, at the internally mounted motor that holds the ware to make it drive the stirring piece and rotate, can wait to detect reagent to the inside of holding the ware and carry out the disturbance, thereby make fluorescence mark and nucleic acid mix together completely, in order to improve the accuracy that detects.

2. The utility model discloses in, at the internally mounted hot plate and the temperature-sensing ware of casing to control it through the switch, can provide suitable temperature for the inside of casing, thereby guarantee to detect that the nucleic acid is in suitable living environment, avoid the temperature to influence the activity of nucleic acid, and then influence going on of detection.

3. The utility model discloses in, the lower surface of casing is provided with the pulley of liftable, accomplishes the back in the detection, through sliding the processing to it, can remove check out test set, has improved detection device's application scope.

4. The utility model discloses in, set up the handle at the upper surface of casing, draw equipment through the handle after using the completion, the convenience is to carrying of equipment, and display screen and projection screen can real-time inside the observation condition of detecting, provide certain convenience for detecting.

Drawings

FIG. 1 is a perspective view of a nucleic acid fluorescence detection device according to the present invention;

FIG. 2 is a schematic structural diagram of a nucleic acid fluorescence detection apparatus according to the present invention;

FIG. 3 is a front view of a nucleic acid fluorescence detection apparatus according to the present invention;

FIG. 4 is a top view of the nucleic acid fluorescence detection device of the present invention.

Illustration of the drawings:

1. a housing; 2. heating plates; 3. a handle; 4. a connecting rod; 5. a fixing plate; 6. a groove; 7. a thermally insulating cavity; 8. a fixed block; 9. a temperature sensor; 10. a first electric push rod; 11. detecting a probe; 12. a containing vessel; 13. a motor; 14. a second electric push rod; 15. a connecting cylinder; 16. a connecting plate; 17. a support bar; 18. a guide bar; 19. a pulley; 20. a placement chamber; 21. a rotating shaft; 22. a stirring sheet; 23. a switch; 24. a shell door; 25. a handle; 26. a projection screen; 27. a display screen; 28. an operation key; 29. and (5) operating a panel.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention; the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance, and furthermore, unless otherwise explicitly stated or limited, the terms "mounted," "connected," and "connected" are to be construed broadly and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.

Referring to fig. 1-4, the present invention provides an embodiment: a nucleic acid fluorescence detection device comprises a shell 1, wherein a heating plate 2 is fixedly connected to the inner wall of the shell 1, a containing vessel 12 is arranged on the inner wall of the lower end of the shell 1, a containing cavity 20 is fixedly connected to the inner wall of the containing vessel 12, a motor 13 is fixedly connected to the inner wall of the containing cavity 20, a rotating shaft 21 extends from one end of the motor 13, a stirring sheet 22 is fixedly connected to the rotating shaft 21 and penetrates through the containing cavity 20, the motor 13 is installed inside the containing vessel 12 and drives the stirring sheet 22 to rotate, a reagent to be detected inside the containing vessel 12 can be disturbed, so that a fluorescent marker and nucleic acid are completely mixed together to improve the detection accuracy, a heat insulation cavity 7 is arranged on the inner wall of the upper end of the shell 1, a first electric push rod 10 is fixedly connected to the inner wall of the heat insulation cavity 7, a detection probe 11 is fixedly connected to the first electric push rod 10, a fixing block 8 is arranged on the inner wall of the upper end of the shell 1 and positioned on the right side of the heat insulation cavity 7, the lower surface of the fixed block 8 is provided with a temperature sensor 9, the heating plate 2 and the temperature sensor 9 are arranged in the shell 1 and controlled by the switch 23, and the proper temperature can be provided for the interior of the shell 1, so that the nucleic acid to be detected is ensured to be in a proper living environment, the temperature is prevented from influencing the activity of the nucleic acid, and the detection is further influenced;

the upper surface of the shell 1 is provided with a groove 6, the side wall of the groove 6 is fixedly connected with a fixing plate 5, one side of the fixing plate 5 is fixedly connected with a connecting rod 4, the outer wall of the connecting rod 4 is rotatably connected with a handle 3, the upper surface of the shell 1 is provided with the handle 3, the equipment is extracted through the handle 3 after use, the equipment is convenient to carry, the lower surface of the shell 1 is fixedly connected with a connecting cylinder 15, the inner wall of the connecting cylinder 15 is fixedly connected with a second electric push rod 14, one end of the second electric push rod 14 is fixedly connected with a connecting plate 16, the lower surface of the connecting plate 16 is fixedly connected with a supporting rod 17, one end of the supporting rod 17 is fixedly connected with a guide rod 18, the outer wall of the guide rod 18 is rotatably connected with a pulley 19, the lower surface of the shell 1 is provided with a liftable pulley 19, after detection is finished, the detection equipment can be moved by sliding the liftable pulley 19, the application range of the detection device is improved, the surface of the shell 1 is rotatably connected with two sets of shell doors 24 through hinges, the surface of each shell door 24 is provided with a handle 25, the surface of each shell door 24 is provided with a projection screen 26, the surface of each shell door 24 is provided with an operation panel 29, the surface of each operation panel 29 is provided with a display screen 27, the display screens 27 and the projection screens 26 can observe the internal detection condition in real time, certain convenience is provided for detection, the surface of each operation panel 29 is provided with an operation key 28 below the display screen 27, the surface of each operation panel 29 is provided with a switch 23 below the operation key 28, and the switch 23 is electrically connected with the temperature sensor 9, the first electric push rod 10, the second electric push rod 14 and the motor 13.

The working principle is as follows: the shell door 24 is opened by a handle 25 on the surface of the shell door 24, and then the nucleic acid reagent with the fluorescent label to be detected is placed in the containing dish 12 inside the shell body 1, then the shell door 24 is closed and the heating plate 2 is heated through the switch 23, so that the inside of the shell 1 has a proper temperature, then the first electric push rod 10 is operated to drive the detection probe 11 into the inside of the containing dish 12, so as to realize the fluorescence detection of the nucleic acid inside the containing dish 12, when the detection probe 11 detects the nucleic acid fluorescence, the motor 13 is operated to drive the stirring sheet 22 to rotate, thereby disturbing the nucleic acid inside the vessel 12 to ensure sufficient contact between the fluorescent label and the nucleic acid, when the detection is finished, the second electric push rod 14 is operated, the pulley 19 below the second electric push rod extends out of the connecting cylinder 15, and then the movement of the detection device is pushed through the handle 3.

Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications and variations can be made in the embodiments or in part of the technical features of the embodiments without departing from the spirit and the scope of the invention.

Claims

1. A nucleic acid fluorescence detection apparatus comprising a housing (1), characterized in that: the heating plate (2) is fixedly connected to the inner wall of the shell (1), a containing vessel (12) is arranged on the inner wall of the lower end of the shell (1), a containing cavity (20) is fixedly connected to the inner wall of the containing vessel (12), a motor (13) is fixedly connected to the inner wall of the containing cavity (20), a rotating shaft (21) is arranged at one end of the motor (13) in an extending mode, and the rotating shaft (21) penetrates through the containing cavity (20) and is fixedly connected with a stirring sheet (22);

the upper surface of casing (1) is provided with recess (6), lateral wall fixedly connected with fixed plate (5) of recess (6), one side fixedly connected with connecting rod (4) of fixed plate (5), the outer wall of connecting rod (4) rotates and is connected with handle (3), the lower fixed surface of casing (1) is connected with connecting cylinder (15), the inner wall fixedly connected with second electric putter (14) of connecting cylinder (15), the one end fixedly connected with connecting plate (16) of second electric putter (14), the lower fixed surface of connecting plate (16) is connected with bracing piece (17), the one end fixedly connected with guide arm (18) of bracing piece (17), the outer wall rotation of guide arm (18) is connected with pulley (19).

2. The nucleic acid fluorescence detection apparatus according to claim 1, characterized in that: the upper end inner wall of casing (1) is provided with thermal-insulated chamber (7), the inner wall fixedly connected with electric putter (10) in thermal-insulated chamber (7), electric putter (10) run through thermal-insulated chamber (7) fixedly connected with test probe (11).

3. The nucleic acid fluorescence detection apparatus according to claim 1, characterized in that: the upper end inner wall of casing (1) just is located the right side in thermal-insulated chamber (7) and is provided with fixed block (8), the lower surface of fixed block (8) is provided with temperature-sensing ware (9).

4. The nucleic acid fluorescence detection apparatus according to claim 1, characterized in that: the surface of the shell (1) is rotatably connected with two groups of shell doors (24) through hinges, and the surface of each shell door (24) is provided with a handle (25).

5. The nucleic acid fluorescence detection apparatus according to claim 4, characterized in that: a projection screen (26) is arranged on the surface of one group of the shell doors (24), and an operation panel (29) is arranged on the surface of one group of the shell doors (24).

6. The nucleic acid fluorescence detection apparatus according to claim 5, characterized in that: a display screen (27) is arranged on the surface of the operation panel (29), and operation keys (28) are arranged on the surface of the operation panel (29) and below the display screen (27).

7. The nucleic acid fluorescence detection apparatus according to claim 5, characterized in that: a switch (23) is arranged on the surface of the operation panel (29) and below the operation key (28).

8. The nucleic acid fluorescence detection apparatus according to claim 7, characterized in that: the switch (23) is electrically connected with the temperature sensor (9), the first electric push rod (10), the second electric push rod (14) and the motor (13).