CN214115403U - Rapid extraction and preparation device for monoclonal antibody - Google Patents

Abstract

The utility model discloses a monoclonal antibody's preparation facilities that draws fast, the power distribution box comprises a box body, four support columns of box lower wall fixedly connected with, two boxes are placed to two fixedly connected with of box upper wall the case lower wall of placing all communicates and is equipped with the catheter, two the catheter all runs through the box upper wall, box lower wall intercommunication is equipped with the discharging pipe, be equipped with the valve on the discharging pipe, be equipped with reciprocating mechanism on the box, be equipped with wiper mechanism on the box. The utility model discloses can realize dropping into an amount of serum and normal saline at every turn, and need not the manual work and measure at every turn, simplify operating personnel's operating procedure, and then greatly reduced operating personnel's working strength, and can not be because the manual work measures and produce the error, guaranteed the device's antibody and draw the effect, can also wash the box is inside, guaranteed the device's cleanness, and easy operation, improved people's cleaning efficiency.

Description

Rapid extraction and preparation device for monoclonal antibody

Technical Field

The utility model relates to an antibody extraction element technical field especially relates to a monoclonal antibody's preparation facilities that draws fast.

Background

Monoclonal antibodies are highly homogeneous antibodies produced by a single B cell clone that are directed against only a particular epitope. Usually, the antibody is prepared by hybridoma technique, and after the antibody preparation is completed, the antibody is generally extracted by salting out method, and a certain amount of physiological saline and serum solution is added each time to precipitate the antibody.

When adding normal saline and serum in traditional antibody extraction element, need the volume of manual measurement serum and normal saline at every turn, make and add a certain amount of normal saline and serum at every turn, complex operation, thereby operating personnel's working strength has been increased, and manual measurement error exists, it is different to lead to serum and normal saline proportion, thereby influence the effect of salting out, and then influence the antibody and draw the effect, so, need design a monoclonal antibody's quick extraction preparation facilities and solve above-mentioned problem.

SUMMERY OF THE UTILITY MODEL

The utility model aims at solving the defects existing in the prior art and providing a device for rapidly extracting and preparing monoclonal antibodies.

In order to achieve the above purpose, the utility model adopts the following technical scheme:

the utility model provides a monoclonal antibody draws preparation facilities fast, includes the box, four support columns of box lower wall fixedly connected with, two of box upper wall fixedly connected with place the case, two it all communicates and is equipped with the catheter to place the case lower wall, two the catheter all runs through the box upper wall, box lower wall intercommunication is equipped with the discharging pipe, be equipped with the valve on the discharging pipe, be equipped with reciprocating mechanism on the box, be equipped with wiper mechanism on the box.

Preferably, reciprocating mechanism includes the motor of fixed connection at the box lateral wall, the output shaft fixedly connected with pivot of motor, pivot lateral wall fixedly connected with cam, two dead levers of top fixedly connected with in the box, be equipped with the movable plate in the box, two movable plate upper wall fixedly connected with carriage release lever, two equal fixedly connected with piston in movable plate upper end, the movable plate upper wall passes through spring and box inner top elastic connection.

Preferably, wiper mechanism includes and rotates the rotation section of thick bamboo of being connected through rotary seal circle and box internal top, it is equipped with a plurality of shower nozzles to rotate a section of thick bamboo lateral wall through water pipe intercommunication, box upper wall fixedly connected with water pump, the end and the inlet tube intercommunication of intaking of water pump, the play water end and the connecting pipe intercommunication of water pump, the connecting pipe runs through the box upper wall and communicates with a rotation section of thick bamboo, the first conical gear of one end fixedly connected with of motor is kept away from in the pivot, it has second conical gear to rotate a section of thick bamboo lateral wall fixedly connected with, first conical gear and second conical gear meshing.

Preferably, the cross section of each of the two fixing rods is an inverted T-shaped structure, and the two fixing rods penetrate through the side wall of the moving plate.

Preferably, the two pistons are respectively positioned in the two liquid guide pipes, and the two pistons are respectively in sealing sliding connection with the inner walls of the two liquid guide pipes.

Preferably, the cam side wall is contacted with the lower wall of the moving plate, and the contact position of the cam and the lower wall of the moving plate is located in the middle of the moving plate.

The utility model discloses in there mainly have beneficial effect:

1. the utility model drives the rotating shaft to rotate through the motor, the rotating shaft drives the cam to rotate, the cam is matched with the elastic action of the spring in the rotating process, so that the moving plate moves up and down, the moving plate drives the moving rod to move, the moving rod drives the piston to move, when the piston leaves the catheter, the normal saline and the serum solution in the placing box enter the box body along the catheter, mixing in the box body, driving the movable rod to move downwards in the process of moving downwards by the movable plate, driving the piston to move downwards by the movable rod, when the moving plate is contacted with the horizontal end of the fixed rod, the motor is turned off, a certain amount of serum and normal saline can be fed each time, manual measurement each time is not needed, the operation steps of operators are simplified, the working strength of operators is greatly reduced, no error is generated due to manual measurement, and the antibody extraction effect of the device is ensured;

2. the utility model discloses a pivot drives first conical gear and rotates, and first conical gear drives second conical gear and rotates, and second conical gear drives and rotates a section of thick bamboo, rotates a section of thick bamboo and drives the shower nozzle rotation, and the shower nozzle can wash the box inside at the work of pivoted in-process cooperation water pump, has guaranteed the device's cleanness, and easy operation, has improved people's cleaning efficiency.

Drawings

FIG. 1 is a schematic structural diagram of a device for rapid extraction and preparation of monoclonal antibodies according to the present invention;

fig. 2 is a sectional view taken along line a-a of fig. 1.

In the figure: the device comprises a box body 1, a rotating shaft 2, a cam 3, a placing box 4, a catheter 5, a piston 6, a moving rod 7, a moving plate 8, a fixed rod 9, a motor 10, a discharging pipe 11, a first bevel gear 12, a second bevel gear 13, a rotating cylinder 14, a connecting pipe 15, a spray head 16, a water pump 17 and a water inlet pipe 18.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments.

Referring to fig. 1-2, a preparation facilities is drawed fast to monoclonal antibody, including box 1, four support columns of box 1 lower wall fixedly connected with, two of box 4 are placed to box 1 upper wall fixedly connected with, two are placed box 4 lower walls and all communicate and are equipped with catheter 5, and two catheter 5 all run through box 1 upper wall, and box 1 lower wall intercommunication is equipped with discharging pipe 11, is equipped with the valve on the discharging pipe 11.

The box body 1 is provided with a reciprocating mechanism, the reciprocating mechanism comprises a motor 10 fixedly connected with the side wall of the box body 1, an output shaft of the motor 10 is fixedly connected with a rotating shaft 2, the side wall of the rotating shaft 2 is fixedly connected with a cam 3, the side wall of the cam 3 is contacted with the lower wall of a movable plate 8, the contact position of the cam 3 and the lower wall of the movable plate 8 is positioned at the middle position of the movable plate 8, the top part in the box body 1 is fixedly connected with two fixed rods 9, the sections of the two fixed rods 9 are of inverted T-shaped structures, a piston 6 can be prevented from leaving a liquid guide tube 5, the two fixed rods 9 penetrate through the side wall of the movable plate 8, the movable plate 8 is prevented from inclining during moving, the movable plate 8 is arranged in the box body 1, the upper wall of the movable plate 8 is fixedly connected with two movable rods 7, the upper ends of the two movable rods 7 are fixedly connected with pistons 6, the two pistons 6 are respectively positioned in the two liquid guide tubes 5, and the two pistons 6 are respectively connected with the inner walls of the two liquid guide tubes 5 in a sealing and sliding mode, the upper wall of the moving plate 8 is elastically connected with the top in the box body 1 through a spring, so that the moving plate 8 is always kept in contact with the cam 3.

Be equipped with wiper mechanism on box 1, wiper mechanism includes the rotary drum 14 of being connected through rotatory sealing washer and box 1 interior top rotation, 14 lateral walls of rotary drum lead to pipe intercommunication to be equipped with a plurality of shower nozzles 16, box 1 upper wall fixedly connected with water pump 17, the end of intaking of water pump 17 communicates with inlet tube 18, the one end and the outside water tank intercommunication of inlet tube 18 far away from water pump 17, the water outlet end and the connecting pipe 15 intercommunication of water pump 17, connecting pipe 15 runs through box 1 upper wall and communicates with rotary drum 14, the first conical gear 12 of one end fixedly connected with of motor 10 is kept away from to pivot 2, 14 lateral walls of rotary drum fixedly connected with second conical gear 13, first conical gear 12 meshes with second conical gear 13, first conical gear 12 drives second conical gear 13 and rotates, second conical gear 13 drives rotary drum 14 and rotates, rotary drum 14 drives shower nozzle 16 and rotates, shower nozzle 16 can wash box 1 inside at the pivoted in-process.

When the device is used, the motor 10 is started, the motor 10 drives the rotating shaft 2 to rotate, the rotating shaft 2 drives the cam 3 to rotate, the cam 3 is matched with the elastic force action of the spring in the rotating process, so that the moving plate 8 moves up and down, the moving plate 8 drives the moving rod 7 to move up in the upward moving process, the moving rod 7 drives the piston 6 to move up, when the piston 6 leaves the liquid guide tube 5, the normal saline and the serum solution in the two placing boxes 4 enter the box body 1 along the liquid guide tube 5 to be mixed in the box body 1, the moving plate 8 drives the moving rod 7 to move down in the downward moving process, the moving rod 7 drives the piston 6 to move down, when the moving plate 8 is contacted with the horizontal end of the fixed rod 9, the motor 10 is closed, after the normal saline and the serum solution are mixed, the valve is opened, the mixed liquid enters the collecting device along the discharge tube 11, and then the operations are repeated, multiple antibody extractions can be achieved.

After placing the physiological saline and the serum solution in case 4 and using completely, when needing to wash 1 inside of box, only need open water pump 17 and motor 10, water pump 17 makes the water in the water tank get into along inlet tube 18 and connecting pipe 15 and rotates a section of thick bamboo 14 in, and spout through each shower nozzle 16, cooperation motor 10 drives the rotation of pivot 2, pivot 2 drives first conical gear 12 and rotates, first conical gear 12 drives second conical gear 13 and rotates, second conical gear 13 drives and rotates a section of thick bamboo 14 and rotates, it drives shower nozzle 16 and rotates to rotate a section of thick bamboo 14, shower nozzle 16 can wash 1 inside of box at the pivoted in-process.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims (6)

1. The utility model provides a monoclonal antibody draws preparation facilities fast, includes box (1), its characterized in that, four support columns of box (1) lower wall fixedly connected with, box (4) are placed to two fixedly connected with of box (1) upper wall, two it all communicates and is equipped with catheter (5) to place box (4) lower wall, two catheter (5) all run through box (1) upper wall, box (1) lower wall intercommunication is equipped with discharging pipe (11), be equipped with the valve on discharging pipe (11), be equipped with reciprocating mechanism on box (1), be equipped with wiper mechanism on box (1).

2. The device for rapidly extracting and preparing the monoclonal antibody according to claim 1, wherein the reciprocating mechanism comprises a motor (10) fixedly connected to a side wall of the box body (1), an output shaft of the motor (10) is fixedly connected with a rotating shaft (2), a cam (3) is fixedly connected to a side wall of the rotating shaft (2), two fixing rods (9) are fixedly connected to the top of the box body (1), a moving plate (8) is arranged in the box body (1), two moving rods (7) are fixedly connected to the upper wall of the moving plate (8), a piston (6) is fixedly connected to the upper ends of the two moving rods (7), and the upper wall of the moving plate (8) is elastically connected to the top of the box body (1) through a spring.

3. The apparatus for rapid extraction and preparation of monoclonal antibody according to claim 2, the cleaning mechanism comprises a rotating cylinder (14) which is rotationally connected with the inner top of the box body (1) through a rotating sealing ring, a plurality of spray heads (16) are communicated with the side wall of the rotating cylinder (14) through a water pipe, a water pump (17) is fixedly connected with the upper wall of the box body (1), the water inlet end of the water pump (17) is communicated with the water inlet pipe (18), the water outlet end of the water pump (17) is communicated with the connecting pipe (15), the connecting pipe (15) penetrates through the upper wall of the box body (1) and is communicated with the rotating cylinder (14), one end of the rotating shaft (2) far away from the motor (10) is fixedly connected with a first bevel gear (12), a second bevel gear (13) is fixedly connected with the side wall of the rotating cylinder (14), the first bevel gear (12) is meshed with the second bevel gear (13).

4. The apparatus for rapid extraction and preparation of monoclonal antibodies as claimed in claim 2, wherein the cross section of the two fixing rods (9) is inverted T-shaped, and the two fixing rods (9) penetrate the side wall of the moving plate (8).

5. The device for the rapid extraction and preparation of monoclonal antibodies according to claim 2, wherein the two pistons (6) are respectively located in the two liquid guide tubes (5), and the two pistons (6) are respectively connected with the inner walls of the two liquid guide tubes (5) in a sealing and sliding manner.

6. The apparatus for the rapid extraction and preparation of monoclonal antibodies as claimed in claim 2, wherein the side wall of the cam (3) is in contact with the lower wall of the moving plate (8), and the contact position of the cam (3) and the lower wall of the moving plate (8) is located at the middle position of the moving plate (8).

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