CN214115054U - Fluid medium extraction device under anaerobic environment - Google Patents
Fluid medium extraction device under anaerobic environment Download PDFInfo
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- CN214115054U CN214115054U CN202021864071.3U CN202021864071U CN214115054U CN 214115054 U CN214115054 U CN 214115054U CN 202021864071 U CN202021864071 U CN 202021864071U CN 214115054 U CN214115054 U CN 214115054U
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Abstract
The utility model discloses a fluid medium extraction device in anaerobic environment, which comprises a storage container; the inert gas supply unit comprises a nitrogen gas storage cylinder, and a nitrogen gas discharge master control valve is arranged at a gas outlet of the nitrogen gas storage cylinder; the nitrogen gas discharge master control valve is connected with a gas guide pipeline, the tail end of the gas guide pipeline is inserted above the liquid level in the storage container, and a flowmeter and a first control valve are sequentially arranged on the gas guide pipeline from the gas storage bottle to the direction of the storage container; the fluid medium drainage unit comprises a negative pressure drainage device, a stop valve is mounted at the end part of the negative pressure drainage device and connected with a drainage pipeline, the tail end of the drainage pipeline is inserted below the liquid level in the storage container, and an emptying valve and a second control valve are sequentially arranged on the drainage pipeline from the negative pressure drainage device to the storage container; an emptying communication pipeline is arranged between the inert gas supply unit and the fluid medium drainage unit, and a third control valve is arranged on the emptying communication pipeline. The utility model has the advantages of simple structure, convenient operation, strong universality, safety, reliability and the like.
Description
Technical Field
The utility model belongs to the technical field of the water treatment trade, especially, relate to a fluid medium draw-out device under anaerobic environment.
Background
In the experiment process of anaerobic bacteria research, anaerobic bacteria are required to be enriched and domesticated, the incorporation of refractory organic matters is often involved in the process, the process is complex, the toxicity is high, and the workload and the manpower consumption are huge. The strain is usually arranged in a bottle in the implementation process, the bottle opening is sealed by a sealing pad arranged in the bottle cap, a round hole is formed in the bottle cap, a small amount of strain is extracted in a fixed time period (the strain is cultured in nutrient solution), in order to domesticate and enrich characteristic functional bacteria, a corresponding virulent reagent is added into the nutrient solution, and a certain danger is generated in the extraction process. In order to conveniently extract nutrient solution, a bottle is placed on a laboratory bench, two syringe needles with the diameter of 1mm are inserted into a culture bottle through a bottle cover, the syringe needles are long and short, the long syringe needle is inserted into a proper position below the culture solution in the bottle, one end of the long syringe needle is connected with a syringe, the syringe is pushed to the lowest part, and air in the syringe is evacuated; the short needle head is connected with a nitrogen cylinder. The experiment begins, alone is responsible for control nitrogen cylinder switch, slowly open, nitrogen gas pours into the blake bottle into in, alone is responsible for observing the blake bottle, prevent other unexpected condition from appearing in the bottle (for example long needle inserts the bacterial that can not extract the experiment needs deeply too, or long needle inserts inadequately, nitrogen gas directly flows out, or pressure is too big, prop the sealed pad etc. of bad bottle lid), this moment bottle internal pressure risees gradually, when pressure in the bottle equals the frictional force when the syringe extracts, the culture solution slowly pours into the syringe into through long needle in, be responsible for the outflow of observing the nutrient solution in the syringe alone, and suitably stimulate the piston, in order to alleviate nitrogen gas injection volume, when extracting a quantitative, close nitrogen cylinder switch, extract the syringe needle. In the experimental process, three people need to cooperate closely, ensure that nitrogen gas injection pressure is suitable, and the injection volume is too big, and the nutrient solution can be gone over to the other end syringe, can influence experimenter's safety when containing the highly toxic reagent to the culture solution.
In the prior art, most of the experimental operations are still performed by adopting the operation method, if the nutrient solution does not contain toxic reagents, the danger is not high, and if the nutrient solution contains highly toxic medicaments, the operation method has high danger.
Disadvantages of the Current methods of operation
1. Need personnel many, three people cooperate the operation closely to each colleges and universities' scientific research unit all has sufficient scientific research testing personnel, and it is a very big wasting of resources that this experiment link occupies three experimenter, especially under the condition that the experimenter is in short supply, this utility model seems more practical
2. The whole experimental device is assembled by an experimenter in a laboratory, leakage is possible, leakage occurs for a plurality of times in the experimental process, the nitrogen cylinder switch is controlled very accurately, the nitrogen cylinder switch is controlled only by the experience of the experimenter in the experimental process, great uncertainty exists, if the density of the switches is different, if the pressure of the nitrogen cylinder is too high, if the nitrogen cylinder is injected with toxic gas instead of nitrogen gas, and the like, many unsafe factors exist
3. The experiment accessory utilization ratio is not high, and whole device all is equipment temporarily, uses temporarily, does not possess the commonality.
SUMMERY OF THE UTILITY MODEL
To the problems existing in the prior art, the utility model provides a fluid medium extracting device under the anaerobic environment with simple structure, convenient operation, strong universality, safety and reliability.
The utility model discloses a realize like this, a fluid medium draw-out device under anaerobic environment, its characterized in that: the device comprises a storage container for storing fluid media, wherein a sealing plug is arranged at the upper end part of the storage container, two holes are formed in the sealing plug, one hole is an inert gas inlet hole, and the other hole is a drainage hole;
the inert gas supply unit comprises a gas storage bottle for storing inert gas, and a nitrogen gas discharge master control valve is arranged at a gas outlet of the gas storage bottle; the nitrogen gas discharge master control valve is connected with a gas guide pipeline, the tail end of the gas guide pipeline is inserted above the liquid level in the storage container through an inert gas inlet hole, and a flowmeter and a first control valve are sequentially arranged on the gas guide pipeline from a gas storage bottle to the direction of the storage container;
the fluid medium drainage unit comprises a negative pressure drainage device, a stop valve is mounted at the end part of the negative pressure drainage device and connected with a drainage pipeline, the tail end of the drainage pipeline is inserted below the liquid level in the storage container through a drainage hole, and an emptying valve and a second control valve are sequentially arranged on the drainage pipeline from the negative pressure drainage device to the direction of the storage container;
an emptying communication pipeline is arranged between the inert gas supply unit and the fluid medium drainage unit, and a third control valve is arranged on the emptying communication pipeline.
The technical scheme is preferable, the storage device further comprises a closed accommodating box for accommodating the storage container, a movable door is arranged on one side face of the accommodating box, and a sealing strip is arranged between the periphery of the movable door and a doorway; a rubber sealing plug corresponding to the inert gas inlet hole and the drainage hole is arranged at the upper top of the storage container accommodating box, and the air guide pipeline and the drainage pipeline penetrate through the rubber sealing plug; a perspective window is also arranged on one side surface of the accommodating box; the upper portion of holding a side of box is equipped with tail gas discharge port, install the tail gas pipe on the tail gas discharge port, correspond the below of tail gas pipe it has tail gas purification groove to hold to hang on the box.
Above-mentioned technical scheme is preferred, and the negative pressure drainage ware adopts manual plunger negative pressure drainage ware.
Above-mentioned technical scheme is preferred, and negative pressure drainage ware adopts negative pressure drainage bag or negative pressure drainage bottle or negative pressure drainage tube.
The top of the containing box is provided with a nozzle.
The utility model has the advantages that: the utility model provides a device for extracting fluid in anaerobic environment, which can safely and conveniently extract liquid medium and realize accurate quantitative extraction without medium outflow in the extraction process; in actual operation, a single person can finish the extraction of the liquid medium, so that the method is convenient and quick; when the extracted liquid medium contains volatile toxic gas, tail gas treatment is carried out by adding liquid which is easy to absorb harmful gas into the tail gas purification tank, so that the safety of the experimental environment is ensured, in addition, a nozzle is arranged at the top of the containing box, and the absorbing medium can be sprayed into the containing box through the nozzle and is used for absorbing the toxic harmful gas in the containing box or splashing the toxic and harmful liquid; and then guarantee to open the security behind the holding box, in the aspect of installation and use, the utility model has the advantages of simple structure, easily equipment, easy operation are convenient, have the commonality among the similar experiment link that can extend simultaneously.
Drawings
FIG. 1 is a schematic structural diagram of embodiment 1 of the present invention;
fig. 2 is a schematic structural diagram of embodiment 2 of the present invention.
In the figure, 1, a storage container; 2. a sealing plug; 3. an inert gas supply unit; 3-1, gas storage bottles; 3-2, a nitrogen discharge master control valve; 3-3, an air guide pipeline; 3-4, a flow meter; 3-5, a first control valve; 4. a fluid medium drainage unit; 4-1, a negative pressure drainage device; 4-2, a check valve; 4-3, a drainage pipeline; 4-4, an emptying valve; 4-5, a second control valve; 4-6, a third control valve; 5. accommodating the box; 5-1, a movable door; 5-2, sealing strips; 6. And (4) a nozzle.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
Referring to fig. 1, a fluid medium extraction device in an anaerobic environment comprises a storage container 1 for storing a fluid medium, wherein a sealing plug 2 is installed at the upper end of the storage container, and two holes are formed in the sealing plug, wherein one hole is an inert gas inlet hole, and the other hole is a drainage hole;
the inert gas supply unit 3 is used for supplying inert gas which does not participate in reaction to the system, and evacuating air in the system through the inert gas to avoid experiment failure or experiment result deviation caused by air mixed in an anaerobic experiment medium due to air reserved in the system, and meanwhile, the inert gas also has the function of cleaning and evacuating; the device comprises a gas storage bottle 3-1 for storing inert gas, wherein a gas outlet of the gas storage bottle is provided with a nitrogen gas discharge master control valve 3-2; the nitrogen gas discharge master control valve is connected with a gas guide pipeline 3-3, the tail end of the gas guide pipeline is inserted above the liquid level in the storage container through an inert gas inlet hole, a flow meter 3-4 and a first control valve 3-5 are sequentially arranged on the gas guide pipeline from the gas storage bottle to the direction of the storage container, and the flow meter is used for metering the consumption of nitrogen gas; the first control valve is used for controlling nitrogen to enter the storage container 1;
the fluid medium drainage unit 4 is used for extracting and storing a liquid medium in an oxygen-free environment and comprises a negative pressure drainage device 4-1, a check valve 4-2 is installed at the end part of the negative pressure drainage device, the check valve has the function of emptying and stopping on one hand and has the function of sealing and storing after the liquid medium is extracted on the other hand, the check valve is connected with a drainage pipeline 4-3, the tail end of the drainage pipeline is inserted below the liquid level in the storage container through a drainage hole, and an emptying valve 4-4 and a second control valve 4-5 are sequentially arranged on the drainage pipeline from the negative pressure drainage device to the storage container; an emptying communication pipeline is arranged between the inert gas supply unit and the fluid medium drainage unit, and a third control valve 4-6 is arranged on the emptying communication pipeline.
When the same liquid medium is subjected to first extraction operation, the first control valve and the second control valve are closed, the third control valve is opened, and then the emptying valve and the stop valve are opened; after each valve determines the opening and closing state, opening the nitrogen discharge master control valve to supply nitrogen, filling nitrogen in the nitrogen gas guide pipeline and the drainage pipeline, and closing the emptying valve and the nitrogen discharge master control valve; then closing the third control valve and simultaneously opening the first control valve and the second control valve; after the nitrogen gas guide pipeline and the drainage pipeline are closed, the nitrogen gas in the content flows back to the upper part of the storage container, and a closed oxygen-free environment is formed at the moment. Then starting a liquid medium of the negative pressure drainage device to slowly enter the negative pressure drainage device under the guiding action of the drainage pipeline, slowly opening a valve switch of the nitrogen cylinder in the process, firstly closing a nitrogen discharge master control valve of the gas storage cylinder when reaching the sampling set metering, then closing a stop valve, taking down the negative pressure drainage device to move the nutrient solution in the negative pressure drainage device into other containers, or replacing another negative pressure drainage device, and then repeating the above operations.
According to the technical scheme, the negative pressure drainage device is preferably a manual plunger negative pressure drainage device, and a negative pressure drainage bag or a negative pressure drainage bottle or a negative pressure drainage tube which stores negative pressure can be selected; the negative pressure product belongs to a known product, and utilizes a negative pressure blood collection tube; the device can be provided with metering scales for more accurate extraction and realization operation, and is convenient, rapid, safe and reliable.
The above embodiment 1 can be applied to a non-toxic and harmless liquid medium or be operated in a fume hood, and in order to further meet the actual requirement, the utility model can be expanded to the embodiment 2 on the basis of the embodiment 1 no matter in a conditioned laboratory or other places, please refer to fig. 2, the embodiment further comprises a closed accommodating box 5 for placing a storage container, a movable door 5-1 is arranged on one side surface of the accommodating box, and a sealing strip 5-2 is arranged between the periphery of the movable door and a door opening; the upper top of the storage container accommodating box is provided with a rubber sealing plug corresponding to the inert gas inlet hole and the drainage hole, and the air guide pipeline and the drainage pipeline penetrate through the rubber sealing plug, so that the air guide pipeline and the drainage pipeline are tightly attached to the rubber plug, and air leakage is prevented; a perspective window is further arranged on one side face of the accommodating box and used for observing the internal condition of the accommodating box; the upper portion of holding the side of box is equipped with tail gas discharge port, install the tail gas pipe on the tail gas discharge port, correspond the below of tail gas pipe hold and hang on the box and be equipped with tail gas purification groove, deposit the nontoxic liquid or other media of easy liquid medium or volatile toxic gas reaction in the tail gas purification groove, for example be used for absorbing the water of hydrophilic gas, the tail gas of getting rid of like this directly can discharge atmosphere, safe and reliable after through tail gas purification groove.
The top of holding the box is installed nozzle 6, and the purpose of nozzle can be through spraying the poisonous gas that dilutes in holding the box on the one hand, and on the other hand also can hold the inside washing or the disinfection of box under inclosed environment, avoids taking place cross reaction when changing different liquid medium, and unexpected condition takes place appears, for example secondary reaction releases gas or liquid that toxicity is stronger.
In summary, the present invention provides a device for extracting fluid under anaerobic environment, which can safely and conveniently extract liquid medium, and can realize accurate quantitative extraction without any medium outflow during the extraction process; in actual operation, a single person can finish the extraction of the liquid medium, so that the method is convenient and quick; when the extracted liquid medium contains volatile toxic gas, tail gas treatment is carried out by adding liquid which is easy to absorb harmful gas into the tail gas purification tank, so that the safety of the experimental environment is ensured, in addition, a nozzle is arranged at the top of the containing box, and the absorbing medium can be sprayed into the containing box through the nozzle and is used for absorbing the toxic harmful gas in the containing box or splashing the toxic and harmful liquid; and then guarantee to open the security behind the holding box, in the aspect of installation and use, the utility model has the advantages of simple structure, easily equipment, easy operation are convenient, have the commonality among the similar experiment link that can extend simultaneously.
The above description is only exemplary of the present invention and should not be taken as limiting the scope of the present invention, as any modifications, equivalents, improvements and the like made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
Claims (5)
1. A fluid medium extraction device in an anaerobic environment, comprising: the device comprises a storage container for storing fluid media, wherein a sealing plug is arranged at the upper end part of the storage container, two holes are formed in the sealing plug, one hole is an inert gas inlet hole, and the other hole is a drainage hole;
the inert gas supply unit comprises a gas storage bottle for storing inert gas, and a nitrogen gas discharge master control valve is arranged at a gas outlet of the gas storage bottle; the nitrogen gas discharge master control valve is connected with a gas guide pipeline, the tail end of the gas guide pipeline is inserted above the liquid level in the storage container through an inert gas inlet hole, and a flowmeter and a first control valve are sequentially arranged on the gas guide pipeline from a gas storage bottle to the direction of the storage container;
the fluid medium drainage unit comprises a negative pressure drainage device, a stop valve is mounted at the end part of the negative pressure drainage device and connected with a drainage pipeline, the tail end of the drainage pipeline is inserted below the liquid level in the storage container through a drainage hole, and an emptying valve and a second control valve are sequentially arranged on the drainage pipeline from the negative pressure drainage device to the direction of the storage container;
an emptying communication pipeline is arranged between the inert gas supply unit and the fluid medium drainage unit, and a third control valve is arranged on the emptying communication pipeline.
2. The fluid medium extraction device under anaerobic environment of claim 1, wherein: the storage container is characterized by also comprising a closed accommodating box for accommodating the storage container, wherein a movable door is arranged on one side surface of the accommodating box, and a sealing strip is arranged between the periphery of the movable door and a door opening; a rubber sealing plug corresponding to the inert gas inlet hole and the drainage hole is arranged at the upper top of the storage container accommodating box, and the air guide pipeline and the drainage pipeline penetrate through the rubber sealing plug; a perspective window is also arranged on one side surface of the accommodating box; the upper portion of holding a side of box is equipped with tail gas discharge port, install the tail gas pipe on the tail gas discharge port, correspond the below of tail gas pipe it has tail gas purification groove to hold to hang on the box.
3. The fluid medium extraction device under anaerobic environment of claim 1, wherein: the negative pressure drainage device adopts a manual plunger negative pressure drainage device.
4. The fluid medium extraction device under anaerobic environment of claim 1, wherein: the negative pressure drainage device adopts a negative pressure drainage bag or a negative pressure drainage bottle or a negative pressure drainage tube.
5. The fluid medium extraction device under anaerobic environment of claim 2, wherein: the top of the containing box is provided with a nozzle.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021864071.3U CN214115054U (en) | 2020-08-31 | 2020-08-31 | Fluid medium extraction device under anaerobic environment |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021864071.3U CN214115054U (en) | 2020-08-31 | 2020-08-31 | Fluid medium extraction device under anaerobic environment |
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| Publication Number | Publication Date |
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| CN214115054U true CN214115054U (en) | 2021-09-03 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202021864071.3U Active CN214115054U (en) | 2020-08-31 | 2020-08-31 | Fluid medium extraction device under anaerobic environment |
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