CN214032483U - Fluorescent quantitative PCR instrument for gene detection - Google Patents

Fluorescent quantitative PCR instrument for gene detection Download PDF

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Publication number
CN214032483U
CN214032483U CN202022770858.XU CN202022770858U CN214032483U CN 214032483 U CN214032483 U CN 214032483U CN 202022770858 U CN202022770858 U CN 202022770858U CN 214032483 U CN214032483 U CN 214032483U
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China
Prior art keywords
dust removal
removal box
box body
pcr instrument
pcr appearance
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CN202022770858.XU
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Chinese (zh)
Inventor
唐建萍
王万江
马艳
马林花
秦杰
胡永佳
伍琪
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Qinghai Jinyu Medical Laboratory Center Co ltd
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Qinghai Jinyu Medical Laboratory Center Co ltd
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Abstract

The utility model discloses a fluorescence quantitative PCR appearance that can be used to gene detection, including PCR appearance body, be provided with PCR appearance feed inlet on one side surface of PCR appearance body, be provided with the dust removal box on the surface of PCR appearance body, PCR appearance feed inlet is located the inside of dust removal box, the one end opening part hinged joint of dust removal box has the door, fixed mounting has two operating gloves on the inboard surface of door, be provided with the snap close piece on one side surface of dust removal box. This fluorescence quantitative PCR appearance that can be used to gene detection carries out the transfer to the charging tray that waits to detect through the dust removal box, can effectually avoid outside dust to get into the inside of PCR appearance body from PCR appearance feed inlet department, and the effectual outside dust of having avoided causes the pollution to the inside of PCR appearance body to the effectual influence of avoiding the dust to the testing result has strengthened the functional of this PCR appearance simultaneously.

Description

Fluorescent quantitative PCR instrument for gene detection
Technical Field
The utility model relates to a fluorescence quantitative PCR appearance technical field specifically is a fluorescence quantitative PCR appearance that can be used to gene detection.
Background
Coronaviruses belong to the order of nested viruses, the family of coronaviruses, the subfamily of coronaviruses, and are a large family of viruses widely existing in nature; the novel coronavirus is a seventh kind of coronavirus separated by human, belongs to the genus beta, has an envelope, has a circular or elliptical particle shape, and has a gene characteristic which is obviously different from SARS-CoV and MERS-CoV; at present, the whole genome sequence and electron microscope photos of the virus are obtained, in vitro separation culture is successful, in order to quickly detect whether a patient is infected, the check calculation detection is needed, a PCR technology is needed in the detection process, and the real-time Q-PCR technology refers to a method of adding a fluorescent gene into a PCR reaction system, utilizing fluorescent signal accumulation to monitor the whole PCR process in real time, and finally carrying out quantitative analysis on an unknown template through a standard curve.
The in-process of using of current PCR appearance, the staff directly opens flourishing material tray on this equipment, and the dust in the outside air can get into the inside of this equipment from the opening part this moment, produces the pollution to the inside of this equipment to produce the influence to the result of detecting.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a fluorescence quantitative PCR appearance that can be used to gene detection to solve the in-process that current PCR appearance was using among the above-mentioned background art, the staff directly opens the flourishing material tray on this equipment, and the dust in the outside air can follow the inside that the opening part got into this equipment this moment, produces the pollution to the inside of this equipment, thereby produces the problem of influence to the result that detects.
In order to achieve the above object, the utility model provides a following technical scheme: a fluorescent quantitative PCR instrument for gene detection comprises a PCR instrument body, wherein a PCR instrument feed inlet is arranged on the outer surface of one side of the PCR instrument body, a dust removal box body is arranged on the outer surface of the PCR instrument body, the PCR instrument feed inlet is positioned in the dust removal box body, a bin door is hinged to an opening at one end of the dust removal box body, two operating gloves are fixedly arranged on the outer surface of the inner side of the bin door, a locking piece is arranged on the outer surface of one side of the dust removal box body, the bin door and the dust removal box body are fixed through the locking piece, a glass observation window is arranged on the outer surface of the upper end of the dust removal box body, a filter shell is fixedly connected to the outer surface of one side of the dust removal box body, filter cotton is arranged in the filter shell, the filter shell is communicated with the interior of the dust removal box body, and an exhaust pipe is fixedly connected to the outer surface of the other side of the dust removal box body, the exhaust pipe is characterized in that an air suction fan is arranged at an air outlet end of the exhaust pipe, and an electromagnetic valve is fixedly installed in a pipeline of the exhaust pipe.
Preferably, a UV lamp is fixedly arranged on the inner wall of one side of the dust removal box body.
Preferably, a light screen is rotatably arranged on the outer surface of the upper end of the dust removal box body, and the light screen covers the glass observation window.
Preferably, the operating glove is arranged inside the dust removal box body.
Preferably, the dedusting box body is adhered to the PCR instrument body through glue.
Preferably, the bin door is in sealing fit with an opening at one end of the dust removal box body.
Compared with the prior art, the beneficial effects of the utility model are that:
the fluorescent quantitative PCR instrument for gene detection transfers a material disc to be detected through a dust removal box body, the bin door is opened firstly to place the material disc in the dust removal box body, then the bin door is closed, then an air suction fan is started, external air passes through a filter shell to enter the dust removal box body through the rotation of the air suction fan, the external air is filtered by filter cotton in the process of passing through the filter shell, clean air is used for replacing the air in the original dust removal box body at the moment, a feed inlet of the PCR instrument is opened at the moment to stretch out an internal tray, then the material disc is placed on the tray through an operating glove, and then the tray is retracted into the PCR instrument body, so that external dust is effectively prevented from entering the PCR instrument body from the feed inlet of the PCR instrument, the pollution of the external dust to the inside of the PCR instrument body is effectively avoided, and the influence of the dust on a detection result is effectively avoided, meanwhile, the functionality of the PCR instrument is enhanced.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
FIG. 2 is a schematic top view of the present invention;
FIG. 3 is a schematic view of the A-A analysis structure of the present invention;
fig. 4 is a schematic view of the structure of the right side of the present invention.
In the figure: 1. a PCR instrument body; 2. a dust removal box body; 3. a visor; 4. a glass viewing window; 5. a bin gate; 6. operating gloves; 7. an exhaust pipe; 8. an air suction fan; 9. an electromagnetic valve; 10. a fastener; 11. a feed port of a PCR instrument; 12. a filter housing; 13. filtering cotton; 14. and a UV lamp.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-4, the present invention provides a technical solution: a fluorescent quantitative PCR instrument for gene detection comprises a PCR instrument body 1, a PCR instrument feed inlet 11 is arranged on the outer surface of one side of the PCR instrument body 1, a dust removal box body 2 is arranged on the outer surface of the PCR instrument body 1, the PCR instrument feed inlet 11 is positioned in the dust removal box body 2, a bin door 5 is hinged at an opening at one end of the dust removal box body 2, two operating gloves 6 are fixedly arranged on the outer surface of the inner side of the bin door 5, a locking piece 10 is arranged on the outer surface of one side of the dust removal box body 2, the bin door 5 and the dust removal box body 2 are locked and fixed through the locking piece 10, a glass observation window 4 is arranged on the outer surface of the upper end of the dust removal box body 2, a filter shell 12 is fixedly connected on the outer surface of one side of the dust removal box body 2, filter cotton 13 is arranged in the filter shell 12, the filter shell 12 is communicated with the inside of the dust removal box body 2, and an exhaust pipe 7 is fixedly connected on the outer surface of the other side of the dust removal box body 2, an air outlet end of the exhaust pipe 7 is provided with an air suction fan 8, an electromagnetic valve 9 is fixedly installed in a pipeline of the exhaust pipe 7, the air suction fan 8 can transfer a material disc to be detected through the dust removal box body 2, the bin door 5 is firstly opened to place the material disc in the dust removal box body 2, then the bin door 5 is closed, then the air suction fan 8 is started, external air penetrates through the filtering shell 12 to enter the dust removal box body 2 through the rotation of the air suction fan 8, the external air is filtered by the filter cotton 13 in the process of penetrating through the filtering shell 12, the clean air replaces the air in the original dust removal box body 2, a feed port 11 of the PCR instrument is opened at the moment to extend out an internal tray, the material disc is placed on the tray through the operating gloves 6, then the tray is retracted into the PCR instrument body 1, and external dust is effectively prevented from entering the PCR instrument body 1 from the feed port 11, the effectual outside dust of having avoided causes the pollution to PCR appearance body 1's inside to the effectual influence of having avoided the dust to the testing result has strengthened the functional of this PCR appearance simultaneously, installs control panel on the PCR appearance body 1, control panel and suction fan 8, solenoid valve 9 and the 14 electric connection of UV lamp, suction fan 8, solenoid valve 9 and the 14 electric connection external power supply of UV lamp.
Further, fixed mounting has UV lamp 14 on one side inner wall of dust removal box 2, and this can utilize UV lamp 14 to carry out ultraviolet sterilization to the inside of dust removal box 2, the effectual cleanliness of guaranteeing the inside of dust removal box 2.
Further, the outer surface of the upper end of the dust removal box body 2 is rotatably provided with a light screen 3, the light screen 3 covers the glass observation window 4, and the ultraviolet light is prevented from passing through the glass observation window 4 to influence workers.
Further, the operation gloves 6 are arranged inside the dust removing box body 2, which facilitates the loading and unloading operations.
Further, glue adhesion between dust removal box 2 and the PCR appearance body 1, this is convenient for install fixedly dust removal box 2.
Further, door 5 and the sealed laminating of 2 one end openings of dust removal box, this effectual leakproofness of having guaranteed the junction, the while also effectual replacement effect of having guaranteed inside air.
The working principle is as follows: firstly, the device is installed at a specified position, the bin door 5 is opened to place a material tray in the dedusting box body 2, then the bin door 5 is closed and is locked and fixed through the locking piece 10, then the air suction fan 8 is started, the outside air passes through the filter shell 12 to enter the dedusting box body 2 through the rotation of the air suction fan 8, the outside air is filtered by the filter cotton 13 in the process of passing through the filter shell 12, the clean air replaces the air in the original dedusting box body 2, the feed port 11 of the PCR instrument is opened at the moment to stretch out the tray in the inside, then the material tray is placed on the tray through the operating gloves 6, then the tray is retracted into the PCR instrument body 1, the phenomenon that the outside dust enters the PCR instrument body 1 from the feed port 11 of the PCR instrument is effectively avoided, the material tray is taken out after the detection is finished, and then the feed port 11 of the PCR instrument is closed, and opening the bin door 5 again, turning over the light screen 3 and covering the light screen above the glass observation window 4 after a period of time, starting the UV lamp 14, and performing ultraviolet sterilization operation on the inside of the dust removal box body 2 by using ultraviolet rays.
It should be finally noted that the above only serves to illustrate the technical solution of the present invention, and not to limit the scope of the present invention, and that simple modifications or equivalent replacements performed by those skilled in the art to the technical solution of the present invention do not depart from the spirit and scope of the technical solution of the present invention.

Claims (6)

1. The utility model provides a fluorescence quantitative PCR appearance that can be used to gene detection, includes PCR appearance body (1), its characterized in that: the device is characterized in that a PCR instrument feed inlet (11) is arranged on the outer surface of one side of the PCR instrument body (1), a dust removal box body (2) is arranged on the outer surface of the PCR instrument body (1), the PCR instrument feed inlet (11) is positioned inside the dust removal box body (2), a bin door (5) is hinged to an opening at one end of the dust removal box body (2), two operating gloves (6) are fixedly arranged on the outer surface of the inner side of the bin door (5), a locking piece (10) is arranged on the outer surface of one side of the dust removal box body (2), the bin door (5) and the dust removal box body (2) are fixed through the locking piece (10) in a locking way, a glass observation window (4) is arranged on the outer surface of the upper end of the dust removal box body (2), a filtering shell (12) is fixedly connected on the outer surface of one side of the dust removal box body (2), and filtering cotton (13) is arranged inside the filtering shell (12), the dust removal device is characterized in that the filtering shell (12) is communicated with the inside of the dust removal box body (2), an exhaust pipe (7) is fixedly connected to the outer surface of the other side of the dust removal box body (2), an air suction fan (8) is arranged at the air outlet end of the exhaust pipe (7), and an electromagnetic valve (9) is fixedly installed in a pipeline of the exhaust pipe (7).
2. The fluorescence quantitative PCR instrument for gene detection according to claim 1, wherein: and a UV lamp (14) is fixedly arranged on the inner wall of one side of the dust removal box body (2).
3. The fluorescence quantitative PCR instrument for gene detection according to claim 1, wherein: the dust removal box body (2) is characterized in that a light screen (3) is rotatably arranged on the outer surface of the upper end of the dust removal box body, and the light screen (3) covers the glass observation window (4).
4. The fluorescence quantitative PCR instrument for gene detection according to claim 1, wherein: the operation gloves (6) are arranged inside the dust removal box body (2).
5. The fluorescence quantitative PCR instrument for gene detection according to claim 1, wherein: and the dust removal box body (2) is adhered to the PCR instrument body (1) by glue.
6. The fluorescence quantitative PCR instrument for gene detection according to claim 1, wherein: the bin gate (5) is hermetically attached to an opening at one end of the dust removal box body (2).
CN202022770858.XU 2020-11-25 2020-11-25 Fluorescent quantitative PCR instrument for gene detection Active CN214032483U (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202022770858.XU CN214032483U (en) 2020-11-25 2020-11-25 Fluorescent quantitative PCR instrument for gene detection

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202022770858.XU CN214032483U (en) 2020-11-25 2020-11-25 Fluorescent quantitative PCR instrument for gene detection

Publications (1)

Publication Number Publication Date
CN214032483U true CN214032483U (en) 2021-08-24

Family

ID=77362707

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202022770858.XU Active CN214032483U (en) 2020-11-25 2020-11-25 Fluorescent quantitative PCR instrument for gene detection

Country Status (1)

Country Link
CN (1) CN214032483U (en)

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