CN214011261U - Magnetic separation device for 96-well plate type immunoassay - Google Patents
Magnetic separation device for 96-well plate type immunoassay Download PDFInfo
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- CN214011261U CN214011261U CN202022715197.0U CN202022715197U CN214011261U CN 214011261 U CN214011261 U CN 214011261U CN 202022715197 U CN202022715197 U CN 202022715197U CN 214011261 U CN214011261 U CN 214011261U
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Abstract
The utility model provides a magnetic separation device for 96 orifice plate formula immunoassay, include: the magnetic pole comprises a base, wherein a vertical first magnet pole is fixed on one side of the base; the baffle comprises a rectangular bottom plate, a first side plate arranged at one end of the rectangular bottom plate, a second side plate arranged at one end of the rectangular bottom plate, which is adjacent to the first side plate, and a third side plate arranged at the other end of the rectangular bottom plate, which is opposite to the second side plate; the first side plate, the second side plate and the third side plate are positioned on the same side of the rectangular bottom plate, and the other side of the rectangular bottom plate is connected with the other side of the base; and the pin is arranged on the second side plate. The utility model provides a problem that exists when magnetism separation in the magnetic immunoassay process, simple structure, convenient to carry, the operation is simple and direct, can the wide application in a great deal of fields such as food sanitation, biomedicine, environmental monitoring, purposes such as separation, purification, enrichment are realized.
Description
Technical Field
The utility model belongs to the technical field of microbiology and food science experimental device, concretely relates to a magnetic separation device for 96 orifice plate formula immunoassay.
Background
The statements in this section merely provide background information related to the present disclosure and may not necessarily constitute prior art.
Immunomagnetic beads are widely used in immunoassay, and a magnetic separation device is a necessary device for magnetic immunoassay. The magnetic beads are coupled with protein and the like to form magnetic bead conjugates, the conjugates with the magnetic beads are quickly and intensively adsorbed at the bottom of a 96-well plate through a magnetic separation device, and the purposes of solid-liquid separation, impurity removal and purification can be effectively achieved.
For 96-well plate immunoassays, current magnetic separation devices suffer from several disadvantages: the magnets of some magnetic separation devices cannot correspond to the 96-well plate one by one, which is not favorable for the stability of the experiment; some magnetic separation devices are of a four-side surrounding type, which is not beneficial to putting in and taking out a 96-well plate, and is not beneficial to the convenience of operation; some magnetic separation devices are not fixed firmly, and when separation liquid is discarded or the separation liquid is beaten, the 96-well plate can displace, which is not favorable for the accuracy of the experiment.
SUMMERY OF THE UTILITY MODEL
In order to solve the problem, the utility model provides a magnetic separation device for 96 orifice plate formula immunoassay, the utility model provides a problem that exists when the magnetism separates among the magnetic immunoassay process, simple structure, convenient to carry, the operation is simple and direct, can the wide application in a great deal of fields such as food hygiene, biomedicine, environmental monitoring, purposes such as separation, purification, enrichment are realized.
According to some embodiments, the present invention provides a magnetic separation device for 96-well plate immunoassay, which adopts the following technical scheme:
a magnetic separation device for use in a 96-well plate immunoassay, comprising:
the magnetic pole comprises a base, wherein a vertical first magnet pole is fixed on one side of the base;
the baffle comprises a rectangular bottom plate, a first side plate arranged at one end of the rectangular bottom plate, a second side plate arranged at one end of the rectangular bottom plate, which is adjacent to the first side plate, and a third side plate arranged at the other end of the rectangular bottom plate, which is opposite to the second side plate; the first side plate, the second side plate and the third side plate are positioned on the same side of the rectangular bottom plate, and the other side of the rectangular bottom plate is connected with the other side of the base;
and the pin is arranged on the second side plate.
As a further technical limitation, a magnetic separation device for a 96-well plate immunoassay further comprises a 96-well plate, wherein the 96-well plate is provided with pin sites matching the positions of the pins on the second side plate.
Further, the first magnet columns are arranged to be small cylindrical magnets with the diameter of 5.0mm and the height of 10.0mm, and the number of the small cylindrical magnets is 96.
Further, the heights of the first magnet columns are kept consistent; one bottom surface of each first magnet column is fixed on the base, and the other bottom surface of each first magnet column is in one-to-one contact with the center of the bottom surface of the 96 hole plate.
As a further technical limitation, the base is made of hard plastics.
As a further technical limitation, the pin is provided with a second magnetic column with the diameter of 4.0mm and the height of 10.0 mm.
As a further technical limitation, the base is configured to be rectangular.
Furthermore, the length and the width of the rectangle of the base are both smaller than the length and the width of the rectangular bottom plate.
As a further technical limitation, the vertical distance between the first side plate and the base is set to be 7.0 mm.
As a further technical limitation, the vertical distance between the second side plate and the third side plate and the base is set to be 3.5 mm.
Compared with the prior art, the beneficial effects of the utility model are that:
the first magnet columns in the magnetic separation device for 96-hole plate type immunoassay provided by the utility model are contacted with the positive centers of the bottom surfaces of the holes of the 96-hole plate one by one, which is beneficial to the stability of different holes during magnetic separation; three side plates are arranged on the baffle plate, so that the 96-hole plate can be conveniently taken and placed; meanwhile, when the magnetic separation device is combined with the 96-hole plate, the lower half part of the 96-hole plate is matched with the baffle plate, the second side plate is connected with the 96-hole plate through the pin, relative sliding and even separation between the second side plate and the 96-hole plate in the horizontal direction are avoided, and the magnetic separation device cannot slide or fall off when being used in an inverted mode.
Drawings
The accompanying drawings, which form a part of the specification, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention without unduly limiting the scope of the invention.
Fig. 1 is a schematic top view of a magnetic separation device for 96-well plate immunoassay according to an embodiment of the present invention;
fig. 2 is a schematic right-view structural diagram of a 96-well plate according to a first embodiment of the present invention;
fig. 3 is a schematic right-view structural diagram of a magnetic separation device for 96-well plate immunoassay according to an embodiment of the present invention;
FIG. 4 is a schematic diagram of another right-side view of a magnetic separation device for a 96-well plate immunoassay according to a first embodiment of the present invention;
the device comprises a base, a first side plate, a second side plate, a third side plate, a base, a rectangular bottom plate, a first magnet column, a pin, 8 and 96 pore plates, wherein the base comprises a base 1, a first side plate, a second side plate, a third side plate, a fourth side plate, a base 5, a rectangular bottom plate, a fourth side plate, a fifth side plate, a sixth side plate, a base, a fifth side plate, a sixth side plate, a fourth side plate, a base, a fourth side plate, a base, a fourth, a base, a fourth, a fourth, a fourth, a.
The specific implementation mode is as follows:
the present invention will be further explained with reference to the accompanying drawings and examples.
It should be noted that the following detailed description is exemplary and is intended to provide further explanation of the invention. Unless defined otherwise, all technical and scientific terms used herein have the same meaning as commonly understood by one of ordinary skill in the art to which this invention belongs.
It is noted that the terminology used herein is for the purpose of describing particular embodiments only and is not intended to be limiting of example embodiments in accordance with the invention. Terms such as "upper", "lower", "left", "right", "front", "back", "vertical", "horizontal", "side", "bottom", etc. indicate the orientation or position relationship based on the drawings, and are only terms determined for convenience of describing the structural relationship of the components or elements of the present invention, and are not intended to refer to any components or elements of the present invention, and should not be construed as limiting the present invention.
In the present invention, terms such as "fixedly connected", "connected", and the like are to be understood in a broad sense, and may be fixedly connected, or may be integrally connected or detachably connected; may be directly connected or indirectly connected through an intermediate. The meaning of the above terms in the present invention can be determined according to specific situations by persons skilled in the art, and should not be construed as limiting the present invention.
In the case of conflict, the embodiments and features of the embodiments of the present invention can be combined with each other.
Example one
This example describes a magnetic separation device for use in a 96 well plate immunoassay.
A magnetic separation device for a 96-well plate immunoassay as shown in fig. 1, fig. 2, fig. 3 and fig. 4, comprising a first side plate 1, a second side plate 2, a third side plate 3, a base 4, a rectangular bottom plate 5, a first magnet column 6, a pin 7 and a 96-well plate 8; the first side plate 1 is arranged at one end of a rectangular bottom plate 5, the second side plate 2 is arranged at one end of the rectangular bottom plate 5 adjacent to the first side plate 1, and the third side plate is arranged at one end of the rectangular bottom plate 5 opposite to the second side plate 2; meanwhile, the first side plate 1, the second side plate 2 and the third side plate 3 are arranged at the same side of the rectangular bottom plate 5, and the other side of the rectangular bottom plate 5 is connected with the base 4; a vertical first magnet column 4 is fixed on one side of the base 4 far away from the rectangular bottom plate 5; the 96-hole plate 8 is connected with the second side plate 2 through a pin 7, and a pin point matched with the pin 7 on the second side plate 2 is arranged on the 96-hole plate 8; one bottom surface of each first magnet column 6 is fixed on the base 4, and the other bottom surface of each first magnet column is in one-to-one contact with the center of the bottom surface of each 96 hole of the 96 hole plate 8; the base 4 is set to be rectangular, and the length and the width of the rectangle of the base 4 are both smaller than those of the rectangular bottom plate 5.
As an embodiment, the number of the first magnet columns 6 is 96, and the heights of the first magnet columns 6 are kept consistent;
in one embodiment, the base 4 is made of hard plastic.
In one embodiment, the first magnet columns 6 are small cylindrical magnets with a diameter of 5.0mm and a height of 10.0mm, and the first magnet columns 6 are equally spaced apart from each other by 4.0 mm.
As an embodiment, the pin 7 is provided with a second magnetic column with the diameter of 4.0mm and the height of 10.0mm, and under the action of the second magnetic column, the pin 7 can be fixed in the pin site under the adsorption action of a magnetic field, so that the falling-off of the position cannot happen during the use process, and the device is more stable.
In one embodiment, the vertical distance between the first side plate 1 and the base 4 is set to 7.0mm, and the vertical distance between the second side plate 2 and the base 4 is set to 3.5 mm.
As an embodiment, the length of the rectangular bottom plate 5 is set to 120.0mm, and the width is set to 87.0 mm; the length of the base 4 is set to 113.0mm, the width is set to 80.0mm, and the height is set to 6.0 mm.
As an embodiment, the vertical distance from the center of the pin point in the second barrier 2 to the upper edge line of the second barrier 2 is set to 5.0mm, and the vertical distance to the first barrier 1 is set to 56.0 mm.
Example two
This example is an application of the magnetic separation device for a 96-well plate immunoassay described in example one.
In the magnetic bead ELISA experiment, the magnetic separation and cleaning operation steps of the magnetic separation device for 96-well plate type immunoassay are as follows:
(1) inserting a 96-pore plate (Coring, 3590, a hole needs to be punched at the position of the inserted pin) containing magnetic beads into the pin, fixing, standing for magnetic separation for 2 minutes, then inverting the device, discarding supernatant, beating the supernatant on absorbent paper for three times, and removing the supernatant;
(2) pulling out the pins, taking out the 96-well plate, adding 150 mu L of PBST cleaning solution into each well, and repeating the operation (1);
(3) repeating the operation (2) once to finish washing.
The first magnet columns in the magnetic separation device for 96-hole plate type immunoassay provided by the utility model are contacted with the positive centers of the bottom surfaces of the holes of the 96-hole plate one by one, which is beneficial to the stability of different holes during magnetic separation; three side plates are arranged on the baffle plate, so that the 96-hole plate can be conveniently taken and placed; meanwhile, when the magnetic separation device is combined with the 96-hole plate, the lower half part of the 96-hole plate is matched with the baffle plate, the second side plate is connected with the 96-hole plate through the pin, relative sliding and even separation between the second side plate and the 96-hole plate in the horizontal direction are avoided, and the magnetic separation device cannot slide or fall off when being used in an inverted mode.
The above description is only a preferred embodiment of the present invention and is not intended to limit the present invention, and various modifications and changes may be made by those skilled in the art. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Although the present invention has been described with reference to the accompanying drawings, it is not intended to limit the scope of the present invention, and those skilled in the art should understand that various modifications or variations that can be made by those skilled in the art without inventive work are still within the scope of the present invention.
Claims (10)
1. A magnetic separation device for use in a 96-well plate immunoassay, comprising:
the magnetic pole comprises a base, wherein a vertical first magnet pole is fixed on one side of the base;
the baffle comprises a rectangular bottom plate, a first side plate arranged at one end of the rectangular bottom plate, a second side plate arranged at one end of the rectangular bottom plate, which is adjacent to the first side plate, and a third side plate arranged at the other end of the rectangular bottom plate, which is opposite to the second side plate; the first side plate, the second side plate and the third side plate are positioned on the same side of the rectangular bottom plate, and the other side of the rectangular bottom plate is connected with the other side of the base;
and the pin is arranged on the second side plate.
2. A magnetic separation device for use in a 96-well plate immunoassay according to claim 1 further comprising a 96-well plate, said 96-well plate having peg sites positioned to match the location of the pegs on the second side plate.
3. The magnetic separation device for a 96-well plate-type immunoassay according to claim 2, wherein the first magnetic pillars are provided as small cylindrical magnets having a diameter of 5.0mm and a height of 10.0mm, and the number thereof is set to 96.
4. A magnetic separation device for a 96-well plate immunoassay according to claim 3, wherein the heights of the first magnetic pillars are all kept uniform; one bottom surface of each first magnet column is fixed on the base, and the other bottom surface of each first magnet column is in one-to-one contact with the center of the bottom surface of the 96 hole plate.
5. The magnetic separation device of claim 1, wherein the base is made of a rigid plastic.
6. A magnetic separation device for a 96-well plate immunoassay according to claim 1, wherein the pin is provided as a second magnetic column having a diameter of 4.0mm and a height of 10.0 mm.
7. A magnetic separation device for a 96-well plate immunoassay according to claim 1 wherein said base is configured as a rectangle.
8. The magnetic separation device of claim 7, wherein the base rectangle has a length and a width that are less than the length and the width of the rectangular bottom plate.
9. The magnetic separation device of claim 1, wherein the first side plate is positioned 7.0mm from the base at a vertical distance.
10. A magnetic separation device for a 96-well plate immunoassay according to claim 1, wherein the vertical distance of each of the second side plate and the third side plate from the base is set to 3.5 mm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022715197.0U CN214011261U (en) | 2020-11-20 | 2020-11-20 | Magnetic separation device for 96-well plate type immunoassay |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022715197.0U CN214011261U (en) | 2020-11-20 | 2020-11-20 | Magnetic separation device for 96-well plate type immunoassay |
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| Publication Number | Publication Date |
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| CN214011261U true CN214011261U (en) | 2021-08-20 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202022715197.0U Active CN214011261U (en) | 2020-11-20 | 2020-11-20 | Magnetic separation device for 96-well plate type immunoassay |
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| CN (1) | CN214011261U (en) |
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