CN213995934U

CN213995934U - Supply PBMC to select for use 50ml scale centrifuging tube

Info

Publication number: CN213995934U
Application number: CN202021962638.0U
Authority: CN
Inventors: 张宏奥; 彭丽娟
Original assignee: Allcells Biological Technology Shanghai Co ltd
Current assignee: Allcells Biological Technology Shanghai Co ltd
Priority date: 2020-09-09
Filing date: 2020-09-09
Publication date: 2021-08-20
Anticipated expiration: 2030-09-09

Abstract

The utility model discloses a 50ml graduated centrifuge tube for PBMC separation, which comprises a tube body and a tube cover; the tube cover is movably connected to the upper part of the tube body; the pipe body comprises a reserved part, a body and a pipe bottom; the body is provided with upper scale marks; and a lower scale mark is arranged on the tube bottom. The utility model discloses increase the maneuverability of centrifuging tube, reduced the risk that the laboratory sample pollutes, the observation of the bobbin cell precipitation volume of being convenient for and little liquid volume.

Description

Supply PBMC to select for use 50ml scale centrifuging tube

Technical Field

The utility model belongs to the technical field of experimental facilities, more specifically say, relate to a supply PBMC to divide for use 50ml scale centrifuging tube.

Background

Peripheral Blood Mononuclear Cells (PBMC) are mainly cell populations including a small number of other cells such as lymphocytes, monocytes, hematopoietic stem cells, etc., and are commonly used in basic and clinically relevant in vitro studies.

The traditional method for separating mononuclear cells is Ficoll density gradient centrifugation, which is mainly based on the specific gravity difference of each cell component in blood. After density gradient centrifugation, a plasma layer without cells, a PBMC leucocyte layer, a separation liquid layer and a cell precipitation layer containing granulocytes and erythrocytes are sequentially arranged from top to bottom, and after the plasma layer is discarded, the leucocyte layer is sucked out to obtain the PBMC.

The existing PBMC centrifugal tube in the market at present mainly has the following problems:

1) the content of PBMC in single blood collection is high, the current Ficoll separation system usually reaches 45-50ml liquid/centrifuge tube, but the current mainstream 50ml centrifuge tube has limited residual space in the tube after being filled with the Ficoll separation system, and the tube cover of the centrifuge tube has relatively weak tightness, so that liquid is easy to leak and all experimental materials are polluted.

2) The tube bottom of the centrifugal tube is generally of a flat-bottom structure, the cell precipitation amount is not easy to observe, no scale is left below the tube bottom by 5ml, the volume of a small amount of Buffer after cell resuspension is difficult to grasp, and the risk of errors caused by operations such as subsequent counting is increased.

SUMMERY OF THE UTILITY MODEL

To the not enough of prior art existence, the utility model aims to provide a supply PBMC to divide to select for use 50ml scale centrifuging tube to solve the defect of current technique.

In order to achieve the above purpose, the utility model provides a following technical scheme:

a supplies PBMC to select for use 50ml scale centrifuging tube which characterized in that: comprises a tube body and a tube cover; the tube cover is movably connected to the upper part of the tube body; the pipe body comprises a reserved part, a body and a pipe bottom; the body is provided with upper scale marks; and a lower scale mark is arranged on the tube bottom.

The medial surface of tube cap is provided with 6 rings of screw threads.

In an optimized scheme, the volume of the reserved part is 25 ml; the maximum volume of the upper graduation marks of the body is 50 ml.

In an optimized scheme, the maximum volume of the lower scale line at the bottom of the tube is 5 ml.

According to the optimized scheme, the tube bottom is a pointed tube bottom with an angle of 60 degrees.

According to the optimized scheme, an installation seat is arranged on the upper end face of the pipe cover, two rotating shafts are symmetrically arranged on two sides of the installation seat, and a semi-annular handle is connected between the two rotating shafts; the semi-annular handle and the mounting base form a circle with the area equal to that of the upper end face of the pipe cover after being horizontally placed.

Owing to adopted above-mentioned technical scheme, compare with prior art, the utility model discloses increased the maneuverability of centrifuging tube, reduced the risk that the laboratory sample pollutes, the observation of the bottom of a tube cell precipitation volume of being convenient for and little liquid volume.

The present invention will be further explained with reference to the drawings and examples.

Drawings

Fig. 1 is a schematic structural diagram of an embodiment of the present invention;

fig. 2 is a schematic structural diagram of another embodiment of the present invention.

Detailed Description

Example 1

As shown in FIG. 1, a 50ml graduated centrifuge tube for PBMC sorting comprises a tube body 2 and a tube cap 3. The medial surface of tube cap is provided with 6 rings of screw threads.

The tube cover 3 is movably connected to the upper part of the tube body 2. The body includes reservation portion, body and socle 1. The body is provided with an upper scale mark. And a lower scale mark is arranged on the tube bottom.

The volume of the reserved part is 25 ml; the maximum volume of the upper graduation marks of the body is 50 ml. The maximum volume of the lower scale line at the bottom of the tube is 5 ml. The tube bottom is a pointed tube bottom with an angle of 60 degrees.

The tube cap of 6 rings of screw threads can the upper portion of the inseparable combination centrifuging tube body, can be used to the fresh PBMC sample of preparing of long-distance transport, avoids the centrifuging tube to drop or intraductal liquid spills because of the tube cap that leads to of colliding with for a long time, also can prevent that PBMC from selecting separately the in-process liquid and passing through the seepage of tube cap, avoids experimental materials' pollution. Besides the conventional scale of 5-50ml, the pipe body has a reserved volume of 25ml above the scale mark of 50ml, and can prevent overfull liquid from spilling in the operation process. The 5ml scale mark at the bottom of the tube belongs to the fine scale mark, so that the small-volume liquid at the bottom of the tube can be estimated more accurately, and the subsequent cell counting and the cell total amount calculation are facilitated. Compared with a flat tube bottom, the pointed tube bottom can judge the amount of cell sediment more accurately, and cell sediment errors are not easy to be discarded in the process of discarding the supernatant.

The utility model discloses increase the maneuverability of centrifuging tube, reduced the risk that the laboratory sample pollutes, the observation of the bobbin cell precipitation volume of being convenient for and little liquid volume.

Example 2

As shown in FIG. 2, a 50ml graduated centrifuge tube for PBMC sorting comprises a tube body 2 and a tube cap 3. The medial surface of tube cap is provided with 6 rings of screw threads. In order to facilitate rotation and taking, a mounting seat 4 is arranged on the upper end face of the pipe cover, two rotating shafts 5 are symmetrically mounted on two sides of the mounting seat 4, and a semi-annular handle 6 is connected between the two rotating shafts 5. The semi-annular handle and the mounting base form a circle with the area equal to that of the upper end face of the pipe cover after being horizontally placed.

In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "length", "width", "thickness", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", "clockwise", "counterclockwise", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and to simplify the description, but do not indicate or imply that the device or element referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore should not be construed as limiting the present invention.

Furthermore, the terms "first", "second" and "first" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include one or more of that feature. In the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.

The above, only be the concrete implementation of the preferred embodiment of the present invention, but the protection scope of the present invention is not limited thereto, and any person skilled in the art is in the technical scope of the present invention, according to the technical solution of the present invention and the utility model, the concept of which is equivalent to replace or change, should be covered within the protection scope of the present invention.

Claims

1. A supplies PBMC to select for use 50ml scale centrifuging tube which characterized in that: comprises a tube body and a tube cover; the tube cover is movably connected to the upper part of the tube body;

the pipe body comprises a reserved part, a body and a pipe bottom;

the body is provided with upper scale marks; the tube bottom is provided with lower scale marks;

the tube bottom is a pointed tube bottom with an angle of 60 degrees;

the upper end surface of the pipe cover is provided with a mounting seat, two rotating shafts are symmetrically mounted on two sides of the mounting seat, and a semi-annular handle is connected between the two rotating shafts; the semi-annular handle and the mounting base form a circle with the area equal to that of the upper end face of the pipe cover after being horizontally placed.

2. A 50ml calibrated centrifuge tube for PBMC sorting according to claim 1 wherein: the medial surface of tube cap is provided with 6 rings of screw threads.

3. A 50ml calibrated centrifuge tube for PBMC sorting according to claim 1 wherein: the volume of the reserved part is 25 ml; the maximum volume of the upper graduation marks of the body is 50 ml.

4. A 50ml calibrated centrifuge tube for PBMC sorting according to claim 1 wherein: the maximum volume of the lower scale line at the bottom of the tube is 5 ml.