CN213924839U - Gas circuit integrated device of unicellular pretreatment - Google Patents
Gas circuit integrated device of unicellular pretreatment Download PDFInfo
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- CN213924839U CN213924839U CN202022581909.4U CN202022581909U CN213924839U CN 213924839 U CN213924839 U CN 213924839U CN 202022581909 U CN202022581909 U CN 202022581909U CN 213924839 U CN213924839 U CN 213924839U
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Abstract
The utility model relates to a unicellular preceding processing technology field discloses a gas circuit integrated device of unicellular pretreatment, include: the integrated block is provided with an air inlet channel, an air inlet hole group, an air exhaust channel and an air exhaust hole group, the air inlet hole group comprises at least two air inlets, the at least two air inlets are communicated with the air inlet channel, the air exhaust hole group comprises two air exhaust holes, and the two air exhaust holes are communicated with the air exhaust channel; the air pump is respectively communicated with the air inlet channel and the air exhaust channel; and each air inlet hole is provided with a control valve which is configured to control the connection or disconnection of the air inlet hole and the air pump, and each air exhaust hole is provided with a control valve which is configured to control the connection or disconnection of the air exhaust hole and the air pump. The utility model discloses a gas circuit integrated device of unicellular pretreatment has simple structure, gas circuit and integrates the degree and increase and do benefit to the advantage that the modularization set up.
Description
Technical Field
The utility model relates to a unicellular preceding processing technology field especially relates to a gas circuit integrated device of unicellular preceding processing.
Background
Unicellular nucleic acid processing apparatus is arranged in extracting DNA or RNA in the cell, current unicellular nucleic acid processing apparatus includes chip structure and gas circuit device, the chip is structural to be equipped with two at least application of sample grooves, a waste liquid groove and a sample groove and microchannel, the one end and the application of sample groove intercommunication of microchannel, the other end communicates with waste liquid groove and sample groove respectively, the gas circuit device includes the air pump, admit air the tubule, the admission valve, bleed tubule and bleeder valve, admit air the tubule and bleed the tubule and all communicate with the air pump, every application of sample groove and one admit air the tubule intercommunication, be equipped with an admission valve on every admission tubule, waste liquid groove and sample groove communicate with bleed the tubule respectively, be equipped with an bleeder valve on every bleed tubule.
During the experiment, the required reagent of experiment has been held respectively in every application of sample groove, add the reagent in the application of sample groove in the microchannel according to the order respectively, specifically, open the admission valve on the tubule that admits air that corresponds with the application of sample groove, the tubule that admits air lets in gas in to this application of sample groove, the reagent in the application of sample groove enters the microchannel, reagent gets into back in the microchannel, open an bleeder valve, the tubule of bleeding can be bled so that the liquid reagent in the microchannel flows to the microchannel, because the gas circuit device makes the more and more tubule structure of tubule of gas circuit device comparatively complicated including a plurality of tubules of admitting air and a plurality of tubules of bleeding.
SUMMERY OF THE UTILITY MODEL
Based on it, an object of the utility model is to provide a gas circuit integrated device of unicellular pretreatment, simple structure and gas circuit integrate the degree and increase, do benefit to the modular setting of gas circuit integrated device of unicellular pretreatment.
In order to achieve the purpose, the utility model adopts the following technical proposal:
a gas circuit integrated device for single cell pretreatment comprises: the integrated block is provided with an air inlet channel, an air inlet hole group, an air exhaust channel and an air exhaust hole group, the air inlet hole group comprises at least two air inlet holes, the at least two air inlet holes are communicated with the air inlet channel, the at least two air inlet holes are respectively arranged in one-to-one correspondence with at least two sample adding grooves of the chip structure, the air exhaust hole group comprises two air exhaust holes, the two air exhaust holes are communicated with the air exhaust channel, and the two air exhaust holes are respectively arranged in correspondence with a waste liquid groove and a sample groove of the chip structure; the air pump is respectively communicated with the air inlet channel and the air exhaust channel; the air pump is provided with an air inlet hole, the air inlet hole is provided with a control valve, the control valve is configured to control the connection or disconnection of the air inlet hole and the air pump, the control valve is arranged at the air exhaust hole, and the control valve is configured to control the connection or disconnection of the air exhaust hole and the air pump.
As a preferred scheme of the gas path integration device for single-cell pretreatment, the gas inlet holes include a first gas inlet hole and a second gas inlet hole, the diameter of the second gas inlet hole is smaller than that of the first gas inlet hole, the control valve at the gas inlet hole is a first gas inlet control valve, one end of the first gas inlet control valve extends into the first gas inlet hole and forms a first middle gas inlet cavity and a first bottom gas inlet cavity with the gas inlet holes, the first gas inlet control valve is configured to control the connection or disconnection of the first middle gas inlet cavity and the first bottom gas inlet cavity, the gas inlet channel is communicated with the first middle gas inlet cavity, and the second gas inlet hole is communicated with the first bottom gas inlet cavity.
As a preferred scheme of the gas path integration device for single-cell pretreatment, an air inlet blind hole is formed in the integrated block, a second air inlet control valve is arranged at the air inlet blind hole, one end of the second air inlet control valve extends into the air inlet blind hole and forms a second upper air inlet cavity, a second middle air inlet cavity and a second bottom air inlet cavity with the air inlet blind hole, the second middle air inlet cavity is located between the second upper air inlet cavity and the second bottom air inlet cavity, the second middle air inlet cavity is communicated with the air pump, the second bottom air inlet cavity is communicated with the air inlet channel, the second air inlet control valve is configured to communicate the second upper air inlet cavity with the second middle air inlet cavity when power is off, and communicate the second middle air inlet cavity with the second bottom air inlet cavity when power is on.
As a preferred scheme of the gas circuit integrated device for single-cell pretreatment, a first detection hole is formed in the integrated block and communicated with the second upper air inlet cavity.
As a preferred scheme of the gas circuit integration device for single-cell pretreatment, the two gas extraction holes are respectively a first gas extraction hole and a second gas extraction hole, the first gas extraction hole comprises a first gas extraction communication hole and a first connection blind hole, the first gas extraction communication hole is communicated with the waste liquid tank, the second gas extraction hole comprises a second gas extraction communication hole and a second connection blind hole, the second gas extraction communication hole is communicated with the sample tank, the control valves are respectively arranged in the first connection blind hole and the second connection blind hole, the control valve arranged at the first connection blind hole is a first gas extraction control valve, the first gas extraction control valve and the first connection blind hole form a first upper connection cavity and a first middle connection cavity, the first middle connection cavity is communicated with the first gas extraction communication hole, and the control valve arranged at the second connection blind hole is a second gas extraction control valve, the second air pumping control valve and the second connecting blind hole form a second upper connecting cavity, a second middle connecting cavity and a second bottom connecting cavity, the second middle connecting cavity is located between the second upper connecting cavity and the second bottom connecting cavity, the second upper connecting cavity is communicated with the second air pumping communication hole, the second middle connecting cavity is communicated with the air pumping channel, the second bottom connecting cavity is communicated with the first upper connecting cavity, the second air pumping control valve is configured to be communicated with the second upper connecting cavity and the second middle connecting cavity when power is off, and the second middle connecting cavity is communicated with the second bottom connecting cavity when power is on.
As a preferred scheme of the gas circuit integrated device for single-cell pretreatment, the first gas exhaust control valve further forms a first bottom connection cavity with the first connection blind hole, the first gas exhaust control valve is configured to communicate the first upper connection cavity with the first middle connection cavity when the power is off, communicate the first middle connection cavity with the first bottom connection cavity when the power is on, and a second detection hole communicated with the first bottom connection cavity is arranged on the integrated block.
As a preferred scheme of the gas circuit integration device for single-cell pretreatment, an air exhaust blind hole and a third detection hole are arranged on the integrated block, a third air exhaust control valve is arranged at the air exhaust blind hole, one end of the third air exhaust control valve extends into the air exhaust blind hole and forms an upper air exhaust cavity, a middle air exhaust cavity and a bottom air exhaust cavity with the air exhaust blind hole, the middle air exhaust cavity is located between the upper air exhaust cavity and the bottom air exhaust cavity, the third detection hole is communicated with the upper air exhaust cavity, the middle air exhaust cavity is communicated with the air pump, the bottom air exhaust cavity is communicated with the air exhaust channel, and the third air exhaust control valve is configured to communicate the upper air exhaust cavity with the middle air exhaust cavity when power is off and communicate the middle air exhaust cavity with the bottom air exhaust cavity when power is on.
As an optimal scheme of the gas circuit integrated device for single-cell pretreatment, a detection blind hole and a fourth detection hole are further formed in the integrated block, a detection control valve is arranged at the detection blind hole, the detection control valve and the detection blind hole form a middle detection cavity and a bottom detection cavity, the bottom detection cavity is communicated with the fourth detection hole, the middle detection cavity is communicated with the gas inlet channel, and the detection control valve is configured to control the communication or disconnection of the middle detection cavity and the bottom detection cavity.
As an optimal scheme of the gas circuit integration device for single-cell pretreatment, the number of the gas inlet channels is at least two, the number of the gas inlet hole groups is the same as that of the gas inlet channels, the gas inlet channels are arranged in a one-to-one correspondence mode with the gas inlet hole groups, the number of the gas exhaust channels is at least two, the number of the gas exhaust hole groups is the same as that of the gas exhaust channels, and the gas exhaust channels are arranged in a one-to-one correspondence mode with the gas exhaust hole groups.
As a preferred scheme of the gas circuit integrated device for single cell pretreatment, the gas circuit integrated device for single cell pretreatment further comprises a circuit board, and the circuit board is electrically connected with the control valves respectively.
The utility model has the advantages that: the utility model discloses an integrated package that single cell pretreatment's gas circuit integrated device was add has replaced the tubule of admitting air among the prior art and the tubule of bleeding, the structural application of sample groove intercommunication of inlet port group and chip on the integrated package, the intercommunication or the disconnection of inlet port and air pump can be controlled to the control valve of setting in inlet port department, the structural waste liquid groove and the sample groove intercommunication of bleed port group and chip, the intercommunication or the disconnection of bleed port and air pump can be controlled to the control valve of setting in bleed port department, the device simple structure and the gas circuit degree of integrating increase, be favorable to the modularization setting of device.
Drawings
In order to more clearly illustrate the technical solutions in the embodiments of the present invention, the drawings required to be used in the description of the embodiments of the present invention will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to the contents of the embodiments of the present invention and the drawings without creative efforts.
Fig. 1 is a schematic view of a gas circuit integration device for single-cell pretreatment according to an embodiment of the present invention;
fig. 2 is a schematic diagram of an integrated block provided by an embodiment of the present invention in one direction;
fig. 3 is a schematic diagram of the integrated package provided by the embodiment of the present invention in another direction;
fig. 4 is a cross-sectional view of the integrated package along the direction a-a according to the embodiment of the present invention;
fig. 5 is a cross-sectional view of the gas circuit integration device for single-cell pretreatment according to an embodiment of the present invention;
FIG. 6 is an enlarged view of a portion of FIG. 5 at D;
FIG. 7 is a cross-sectional view of the integrated package along the B-B direction according to the embodiment of the present invention
Fig. 8 is a cross-sectional view of the integrated package along the direction C-C according to the embodiment of the present invention.
In the figure:
1. integrating the blocks; 101. an air intake passage; 102. an air inlet; 103. an air extraction channel; 1041. a first air extraction hole; 10411. a first pumping communication hole; 10412. a first connecting blind hole; 1042. a second air extraction hole; 10421. a second pumping communication hole; 10422. a second connecting blind hole; 105. a blind air inlet hole; 106. a first detection hole; 107. a second detection hole; 108. air exhaust blind holes; 109. a third detection hole; 1010. detecting a blind hole; 1011. a fourth detection hole;
21. a first intake control valve; 2101. a first upper intake chamber; 2102. a first middle intake chamber; 2103. a first bottom intake chamber; 2104. a first inlet valve bore; 2105. a second inlet valve bore; 22. a first air extraction control valve; 23. a second air extraction control valve; 24. a third air extraction control valve; 25. detecting the control valve;
3. a circuit board.
Detailed Description
In order to make the technical problems, technical solutions and technical effects achieved by the present invention more clear, the embodiments of the present invention will be described in further detail with reference to the accompanying drawings, and obviously, the described embodiments are only some embodiments, not all embodiments of the present invention. Based on the embodiments in the present invention, all other embodiments obtained by those skilled in the art without creative efforts belong to the protection scope of the present invention.
In the description of the present invention, it should be noted that the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", and the like indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are only for convenience of description and simplification of description, but do not indicate or imply that the device or element referred to must have a specific orientation, be constructed and operated in a specific orientation, and thus, should not be construed as limiting the present invention. Furthermore, the terms "first" and "second" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance. Wherein the terms "first position" and "second position" are two different positions.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, e.g., as meaning either a fixed connection or a removable connection; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood in specific cases to those skilled in the art.
As shown in fig. 1 to 8, this embodiment provides an air channel integrated device for single-cell pretreatment, the device includes an integrated block 1, an air pump (not shown in the figure), and a plurality of control valves, the integrated block 1 is provided with an air inlet channel 101, an air inlet hole set, an air exhaust channel 103, and an air exhaust hole set, the air inlet hole set includes five air inlet holes 102, the five air inlet holes 102 are all communicated with the air inlet channel 101, the five air inlet holes 102 are respectively arranged corresponding to five sample-adding slots of a chip structure, the air exhaust hole set includes two air exhaust holes, the two air exhaust holes are both communicated with the air exhaust channel 103, the two air exhaust holes are respectively arranged corresponding to a waste liquid slot and a sample slot of the chip structure, the air pump is respectively communicated with the air inlet channel 101 and the air exhaust channel 103, each air inlet hole 102 is provided with a control valve, the control valve is configured to control the connection or disconnection between the air inlet, the control valve is configured to control the connection or disconnection of the air suction hole to the air pump.
It should be noted that, in other embodiments of the present invention, the number of the intake holes 102 included in each intake hole group is not limited to five in this embodiment, and may be other numbers, specifically selected according to actual needs.
The integrated package 1 that the gas circuit integrated device of unicellular pretreatment add that this embodiment provided has replaced the tubule of admitting air among the prior art and the tubule of bleeding, the structural application of sample groove intercommunication of inlet port group and chip on the integrated package 1, the intercommunication or the disconnection of inlet port 102 and air pump can be controlled to the control valve of setting in inlet port 102 department, the structural waste liquid groove and the sample cell intercommunication of bleed port group and chip, the intercommunication or the disconnection of bleed port and air pump can be controlled to the control valve of setting in bleed port department, the device simple structure and gas circuit integration degree increase, do benefit to the modularization setting of device.
Further, since the single-cell nucleic acid processing apparatus of the present embodiment includes two chip structures, as shown in fig. 4 and 8, the number of the air inlet channels 101 on the manifold block 1 of the present embodiment is two, the number of the air inlet groups is two, and the two air inlet channels 101 and the two air inlet groups are arranged in one-to-one correspondence, that is, the manifold block 1 has ten air inlet holes 102. As shown in fig. 4 and 8, the number of the air exhaust passages 103 in this embodiment is two, the number of the air exhaust hole groups is two, and the two air exhaust passages 103 and the two air exhaust hole groups are arranged in one-to-one correspondence, that is, there are four air exhaust holes on the manifold block 1. In other embodiments, the number of the gas inlet channels 101, the gas inlet hole groups, the gas exhaust channels 103 and the gas exhaust hole groups is not limited to two in this embodiment, and may be one or more than two, specifically, the number is the same as the number of the chip structures of the single-cell nucleic acid processing apparatus.
Each air intake hole 102 of the present embodiment includes a first air intake hole and a second air intake hole, the diameter of the second air intake hole is smaller than the diameter of the first air intake hole, as shown in fig. 5 and 6, the control valve at the air intake hole 102 is a first air intake control valve 21, one end of the first air intake control valve 21 extends into the first air intake hole and forms a first middle air intake cavity 2102 and a first bottom air intake cavity 2103 with the air intake hole 102, the first air intake control valve 21 is configured to control the connection or disconnection of the first middle air intake cavity 2102 and the first bottom air intake cavity 2103, the air intake channel 101 is communicated with the first middle air intake cavity 2102, and the second air intake hole is communicated with the first bottom air intake cavity 2103.
Specifically, as shown in fig. 6, the first air intake control valve 21 of the present embodiment further forms a first upper air intake cavity 2101 together with the first air intake hole, the first middle air intake cavity 2102 is located between the first upper air intake cavity 2101 and the first bottom air intake cavity 2103, the first air intake control valve 21 is provided with a first air intake valve hole 2104, a second air intake valve hole 2105 and a third air intake valve hole, the first air intake valve hole 2104 communicates with the first upper air intake cavity 2101, the second air intake valve hole 2105 communicates with the first middle air intake cavity 2102, and the third air intake valve hole communicates with the first bottom air intake cavity 2103. When the first intake control valve 21 is de-energized, the first intake valve hole 2104 and the second intake valve hole 2105 communicate, so that the first upper intake chamber 2101 communicates with the first middle intake chamber 2102; when the first air intake control valve 21 is energized, the second air intake valve hole 2105 and the third air intake valve hole communicate, so that the first middle air intake chamber 2102 communicates with the first bottom air intake chamber 2103.
When the sample adding slot of the chip structure needs to be ventilated, the first air inlet control valve 21 is powered on, so that the gas entering the first middle air inlet cavity 2102 through the air inlet channel 101 can enter the first bottom air inlet cavity 2103, and finally enters the sample adding slot through the second air inlet hole, and therefore the reagent in the sample adding slot is led into the micro-channel.
As shown in figures 1, 2 and 4, as shown in fig. 7 and 8, the manifold block 1 of this embodiment is provided with two air inlet blind holes 105 and two first detection holes 106, each air inlet blind hole 105 is provided with a second air inlet control valve (not shown in the figure), one end of the second air inlet control valve extends into the air inlet blind hole 105 and forms a second upper air inlet cavity, a second middle air inlet cavity and a second bottom air inlet cavity with the air inlet blind hole 105, the second middle air inlet cavity is located between the second upper air inlet cavity and the second bottom air inlet cavity, each first detection hole 106 is communicated with one second upper air inlet cavity, the second middle air inlet cavity is communicated with an air pump, the second bottom air inlet cavity is communicated with the air inlet channel 101, the second air inlet control valve is configured to communicate the second upper air inlet cavity with the second middle air inlet cavity when the power is off, and communicate the second middle air inlet cavity with the second bottom air inlet cavity when the power is on.
Specifically, the second air inlet control valve is provided with a fourth air inlet valve hole, a fifth air inlet valve hole and a sixth air inlet valve hole, the fourth air inlet valve hole is communicated with the second upper air inlet cavity, the fifth air inlet valve hole is communicated with the second middle air inlet cavity, and the sixth air inlet valve hole is communicated with the second bottom air inlet cavity. When the second air inlet control valve is powered off, the fourth air inlet valve hole is communicated with the fifth air inlet valve hole, so that the second upper air inlet cavity is communicated with the second middle air inlet cavity, and the pressure of air entering the second middle air inlet cavity by the air pump can be detected through the first detection hole 106; when the second air inlet control valve is powered on, the fifth air inlet valve hole is communicated with the sixth air inlet valve hole, so that the second middle air inlet cavity is communicated with the second bottom air inlet cavity, air discharged by the air pump enters the second bottom air inlet cavity through the second middle air inlet cavity, the fifth air inlet valve hole and the sixth air inlet valve hole in sequence, and the air in the second bottom air inlet cavity enters the first middle air inlet cavity 2102 through the air inlet channel 101.
As shown in fig. 3, 4, 7 and 8, the two pumping holes of each pumping hole group of the present embodiment are a first pumping hole 1041 and a second pumping hole 1042, the first pumping hole 1041 includes a first pumping communication hole 10411 and a first connecting blind hole 10412, the first pumping communication hole 10411 communicates with the waste liquid tank, the second pumping hole 1042 includes a second pumping communication hole 10421 and a second connecting blind hole 10422, the second pumping communication hole 10421 communicates with the sample tank, control valves are respectively disposed in the first connecting blind hole 10412 and the second connecting blind hole 10422, as shown in fig. 5, the control valve disposed at the first connecting blind hole 10412 is a first pumping control valve 22, the first pumping control valve 22 and the first connecting blind hole 10412 form a first upper connecting cavity, a first middle connecting cavity and a first bottom connecting cavity, a second detection hole 107 is disposed on the manifold block 1, the second detection hole 107 communicates with the first bottom connecting cavity, the first middle connection chamber is communicated with the first air exhaust communication hole 10411, the first air exhaust control valve 22 is configured to communicate the first upper connection chamber with the first middle connection chamber when power is off, the first middle connection chamber is communicated with the first bottom connection chamber when power is on, as shown in fig. 6, the control valve disposed at the second connection blind hole 10422 is the second air exhaust control valve 23, the second air exhaust control valve 23 and the second connection blind hole 10422 form a second upper connection chamber, a second middle connection chamber and a second bottom connection chamber, the second middle connection chamber is located between the second upper connection chamber and the second bottom connection chamber, the second upper connection chamber is communicated with the second air exhaust communication hole 10421, the second middle connection chamber is communicated with the air exhaust passage 103, the second bottom connection chamber is communicated with the first upper connection chamber, the second air exhaust control valve 23 is configured to communicate the second upper connection chamber with the second middle connection chamber when power is off, when the power is on, the second middle connecting cavity is communicated with the second bottom connecting cavity.
Specifically, the first control valve 22 of taking out of this embodiment is equipped with first bleed valve opening, second bleed valve opening and the third bleed valve opening, and the chamber intercommunication is connected with first upper portion to the first bleed valve opening, and the chamber intercommunication is connected with first middle part to the second bleed valve opening, and the chamber intercommunication is connected with first bottom to the third bleed valve opening. The second of this embodiment is taken out and is equipped with the fourth valve opening of bleeding, the fifth valve opening of bleeding and the sixth valve opening of bleeding on the control valve 23, and the fourth valve opening of bleeding is connected the chamber intercommunication with second upper portion, and the fifth valve opening of bleeding is connected the chamber intercommunication with the second middle part, and the chamber intercommunication is connected with the second bottom to the sixth valve opening of bleeding.
When the first air exhaust control valve 22 is powered off and the second air exhaust control valve 23 is powered on, the first air exhaust valve hole is communicated with the second air exhaust valve hole, that is, the first upper connecting chamber is communicated with the first middle connecting chamber and the first middle connecting chamber is communicated with the first pumping communication hole 10411, which is equivalent to pumping the waste liquid tank, because the second air exhaust control valve 23 is electrified, the fifth air exhaust valve hole is communicated with the sixth air exhaust valve hole, namely, the second middle connecting cavity is communicated with the second bottom connecting cavity, the air exhaust channel 103 is communicated with the second middle connecting cavity, and the second bottom connecting cavity is communicated with the first upper connecting cavity, that is, the air pump can pump the gas in the waste liquid tank to the air pumping channel 103 through the first air pumping communication hole 10411, the first middle connecting cavity, the second air pumping valve hole, the first upper connecting cavity, the second bottom connecting cavity, the sixth air pumping valve hole, the fifth air pumping valve hole and the second middle connecting cavity in sequence.
When the first air pumping control valve 22 is powered on, the second bottom connecting cavity is not communicated with the first upper connecting cavity at this time, that is, the air pump cannot continue to pump the air in the waste liquid tank, because the second air pumping valve hole is communicated with the third air pumping valve hole, the first middle connecting cavity is communicated with the first bottom connecting cavity, the first middle connecting cavity is communicated with the first air pumping communication hole 10411, the first air pumping communication hole 10411 is communicated with the waste liquid tank, and the second detection hole 107 is communicated with the first bottom connecting cavity, therefore, the pressure of the air in the waste liquid tank can be detected through the second detection hole 107.
When the second air pumping control valve 23 is powered off, the fourth air pumping valve hole is communicated with the fifth air pumping valve hole, namely, the second upper connecting cavity is communicated with the second middle connecting cavity, the air pumping channel 103 is communicated with the second middle connecting cavity, and the second upper connecting cavity is communicated with the second air pumping communication hole 10421, because the second middle connecting cavity is not communicated with the second bottom connecting cavity, the air pump can not pump the gas in the waste liquid tank, at the moment, the air pump can pump the gas in the sample tank to the air pumping channel 103 through the second air pumping communication hole 10421 in sequence, the second upper connecting cavity, the fifth air pumping valve hole, the fourth air pumping valve hole, and the second middle connecting cavity.
As shown in fig. 7, the integrated package 1 of the present embodiment is provided with two air-extracting blind holes 108 and two third detection holes 109, as shown in fig. 1, each air-extracting blind hole 108 is provided with a third air-extracting control valve 24, one end of the third air-extracting control valve 24 extends into the air-extracting blind hole 108 and forms an upper air-extracting cavity, a middle air-extracting cavity and a bottom air-extracting cavity with the air-extracting blind hole 108, the middle air-extracting cavity is located between the upper air-extracting cavity and the bottom air-extracting cavity, one third detection hole 109 is communicated with one upper air-extracting cavity, the middle air-extracting cavity is communicated with the air pump, the bottom air-extracting cavity is communicated with the air-extracting channel 103, the third air-extracting control valve 24 is configured such that the upper air-extracting cavity is communicated with the middle air-extracting cavity when the power is off, and the middle air-extracting cavity is communicated with the bottom air-extracting cavity when the power is on.
Specifically, the third air exhaust control valve 24 of this embodiment is provided with a seventh air exhaust valve hole, an eighth air exhaust valve hole and a ninth air exhaust valve hole, wherein the seventh air exhaust valve hole is communicated with the upper air exhaust cavity, the eighth air exhaust valve hole is communicated with the middle air exhaust cavity, and the ninth air exhaust valve hole is communicated with the bottom air exhaust cavity.
When the third air pumping control valve 24 is powered off, the seventh air pumping valve hole is communicated with the eighth air pumping valve hole, namely, the upper air pumping cavity is communicated with the middle air pumping cavity, the air pump is communicated with the upper air pumping cavity through the middle air pumping cavity, and the pressure of the air in the upper air pumping cavity can be detected through the third detection hole 109; when the third air pumping control valve 24 is powered on, the eighth air pumping valve hole is communicated with the ninth air pumping valve hole, i.e. the middle air pumping cavity is communicated with the bottom air pumping cavity, and at the moment, the air pump pumps air in the air pumping channel 103 through the middle air pumping cavity and the bottom air pumping cavity.
As shown in fig. 1, fig. 2, fig. 4, fig. 7 and fig. 8, the integrated package 1 of this embodiment is further provided with two detection blind holes 1010 and two fourth detection holes 1011, each air inlet channel 101 corresponds to one detection blind hole 1010 and one fourth detection hole 1011, as shown in fig. 1, each detection blind hole 1010 is provided with one detection control valve 25, each detection control valve 25 and the detection blind hole 1010 form a middle detection cavity and a bottom detection cavity, the bottom detection cavity is communicated with the fourth detection hole 1011, the middle detection cavity is communicated with the air inlet channel 101, and the detection control valves 25 are configured to control the communication or disconnection of the middle detection cavity and the bottom detection cavity.
Specifically, when the detection control valve 25 is opened, the middle detection chamber and the bottom detection chamber are communicated, and the pressure of the gas in the bottom detection chamber can be detected through the fourth detection hole 1011; when the detection control valve 25 is closed, the middle detection chamber is not communicated with the bottom detection chamber, and the pressure of the gas in the bottom detection chamber cannot be detected through the fourth detection hole 1011 at this time.
As shown in fig. 1 and fig. 5, the gas circuit integrated device for single-cell pretreatment of the present embodiment further includes a circuit board 3, and the circuit board 3 is electrically connected to ten first gas inlet control valves 21, two second gas inlet control valves, two first gas exhaust control valves 22, two second gas exhaust control valves 23, two third gas exhaust control valves 24, and two detection control valves 25, respectively.
When the gas circuit integrated device for single-cell pretreatment of this embodiment lets in gas in to the sample adding groove, first air inlet control valve 21 is electrified, second air inlet control valve is electrified, the gas of air pump combustion gas passes through the second middle part chamber of admitting air in proper order this moment, the fifth air inlet valve hole, the sixth air inlet valve hole gets into the second bottom chamber of admitting air, the gas in the second bottom chamber of admitting air gets into first middle part chamber 2102 of admitting air through inlet channel 101, the gas in first middle part chamber 2102 of admitting air can get into first bottom chamber 2103 of admitting air, finally get into the sample adding groove through the second inlet port, thereby make the reagent in the sample adding groove let in the microchannel.
When the gas circuit integration device for single-cell pretreatment of this embodiment is used to extract gas in the waste liquid tank, the first air pumping control valve 22 is powered off and the second air pumping control valve 23 is powered on, and the air pump can pump the gas in the waste liquid tank to the air pumping channel 103 through the first air pumping communication hole 10411, the first middle connection cavity, the second air pumping valve hole, the first upper connection cavity, the second bottom connection cavity, the sixth air pumping valve hole, the fifth air pumping valve hole and the second middle connection cavity in sequence.
When the gas circuit integration device for single-cell pretreatment of this embodiment is used to extract gas in the sample tank, the second gas extraction control valve 23 is turned off, and the gas pump can extract gas in the sample tank to make the gas flow sequentially through the second gas extraction communication hole 10421, the second upper connection cavity, the fifth gas extraction valve hole, the fourth gas extraction valve hole, and the second middle connection cavity into the gas extraction channel 103.
It should be noted that the foregoing is only a preferred embodiment of the present invention and the technical principles applied. It will be understood by those skilled in the art that the present invention is not limited to the particular embodiments described herein, but is capable of various obvious changes, rearrangements and substitutions as will now become apparent to those skilled in the art without departing from the scope of the invention. Therefore, although the present invention has been described in greater detail with reference to the above embodiments, the present invention is not limited to the above embodiments, and may include other equivalent embodiments without departing from the scope of the present invention.
Claims (10)
1. The utility model provides a gas circuit integrated device of unicellular pretreatment which characterized in that includes:
the integrated chip comprises an integrated block (1), wherein an air inlet channel (101), an air inlet hole group, an air exhaust channel (103) and an air exhaust hole group are arranged on the integrated block (1), the air inlet hole group comprises at least two air inlets (102), the at least two air inlets (102) are communicated with the air inlet channel (101), the at least two air inlets (102) are respectively arranged in one-to-one correspondence with at least two sample adding grooves of a chip structure, the air exhaust hole group comprises two air exhaust holes, the two air exhaust holes are communicated with the air exhaust channel (103), and the two air exhaust holes are respectively arranged in communication correspondence with a waste liquid groove and a sample groove of the chip structure;
the air pump is respectively communicated with the air inlet channel (101) and the air exhaust channel (103);
the air pump is characterized by comprising a plurality of control valves, one control valve is arranged at each air inlet hole (102), the control valves are configured to control the connection or disconnection of the air inlet holes (102) and the air pump, one control valve is arranged at each air exhaust hole, and the control valves are configured to control the connection or disconnection of the air exhaust holes and the air pump.
2. The gas circuit integrated device for single-cell pretreatment according to claim 1, the air inlet holes (102) comprise a first air inlet hole and a second air inlet hole, the diameter of the second air inlet hole is smaller than that of the first air inlet hole, the control valve at the air inlet hole (102) is a first air inlet control valve (21), one end of the first air inlet control valve (21) extends into the first air inlet hole and forms a first middle air inlet cavity (2102) and a first bottom air inlet cavity (2103) with the air inlet hole (102), the first intake control valve (21) is configured to control communication or disconnection of the first middle intake chamber (2102) with the first bottom intake chamber (2103), the air inlet channel (101) is communicated with the first middle air inlet cavity (2102), and the second air inlet hole is communicated with the first bottom air inlet cavity (2103).
3. The gas circuit integrated device for single-cell pretreatment according to claim 2, the manifold block (1) is provided with an air inlet blind hole (105), a second air inlet control valve is arranged at the air inlet blind hole (105), one end of the second air inlet control valve extends into the air inlet blind hole (105) and forms a second upper air inlet cavity, a second middle air inlet cavity and a second bottom air inlet cavity together with the air inlet blind hole (105), the second middle inlet chamber is located between the second upper inlet chamber and the second bottom inlet chamber, the second middle air inlet cavity is communicated with the air pump, the second bottom air inlet cavity is communicated with the air inlet channel (101), the second intake control valve is configured to communicate the second upper intake chamber with the second middle intake chamber when de-energized and to communicate the second middle intake chamber with the second bottom intake chamber when energized.
4. The gas circuit integrated device for single-cell pretreatment according to claim 3, wherein a first detection hole (106) is formed in the integrated block (1), and the first detection hole (106) is communicated with the second upper gas inlet cavity.
5. The gas circuit integration device for single-cell pretreatment according to claim 1, wherein the two air exhaust holes are a first air exhaust hole (1041) and a second air exhaust hole (1042), the first air exhaust hole (1041) comprises a first air exhaust communication hole (10411) and a first connection blind hole (10412), the first air exhaust communication hole (10411) is communicated with the waste liquid tank, the second air exhaust hole (1042) comprises a second air exhaust communication hole (10421) and a second connection blind hole (10422), the second air exhaust communication hole (10421) is communicated with the sample tank, the first connection blind hole (10412) and the second connection blind hole (10422) are respectively provided with the control valve therein, the control valve provided at the first connection blind hole (10412) is a first air exhaust control valve (22), and the first air exhaust control valve (22) and the first connection blind hole (10412) form a first upper connection cavity and a first middle connection cavity, the first middle connecting cavity is communicated with the first air exhaust communicating hole (10411), the control valve arranged at the second connecting blind hole (10422) is a second air exhaust control valve (23), the second air pumping control valve (23) and the second connecting blind hole (10422) form a second upper connecting cavity, a second middle connecting cavity and a second bottom connecting cavity, the second middle connecting cavity is positioned between the second upper connecting cavity and the second bottom connecting cavity, the second upper connecting cavity is communicated with the second air exhaust communication hole (10421), the second middle connecting cavity is communicated with the air exhaust channel (103), the second bottom connection chamber communicates with the first upper connection chamber, and the second evacuation control valve (23) is configured to communicate the second upper connection chamber with the second middle connection chamber when de-energized, and to communicate the second middle connection chamber with the second bottom connection chamber when energized.
6. The gas circuit integrated device for single-cell pretreatment according to claim 5, wherein the first air exhaust control valve (22) further forms a first bottom connection cavity with the first connection blind hole (10412), the first air exhaust control valve (22) is configured to communicate the first upper connection cavity with the first middle connection cavity when power is off, and communicate the first middle connection cavity with the first bottom connection cavity when power is on, and a second detection hole (107) communicated with the first bottom connection cavity is formed in the integrated block (1).
7. The gas circuit integration device for single-cell pretreatment according to claim 5, wherein the manifold block (1) is provided with a blind air-extracting hole (108) and a third detection hole (109), the blind air-extracting hole (108) is provided with a third air-extracting control valve (24), one end of the third air-extracting control valve (24) extends into the blind air-extracting hole (108) and forms an upper air-extracting cavity, a middle air-extracting cavity and a bottom air-extracting cavity with the blind air-extracting hole (108), the middle air-extracting cavity is located between the upper air-extracting cavity and the bottom air-extracting cavity, the third detection hole (109) is communicated with the upper air-extracting cavity, the middle air-extracting cavity is communicated with the gas pump, the bottom air-extracting cavity is communicated with the air-extracting channel (103), and the third air-extracting control valve (24) is configured to communicate the upper air-extracting cavity with the middle air-extracting cavity when power is cut off, and when the power is on, the middle air exhaust cavity is communicated with the bottom air exhaust cavity.
8. The gas circuit integrated device for single-cell pretreatment according to claim 1, wherein a detection blind hole (1010) and a fourth detection hole (1011) are further formed in the integrated block (1), a detection control valve (25) is arranged at the detection blind hole (1010), the detection control valve (25) and the detection blind hole (1010) form a middle detection cavity and a bottom detection cavity, the bottom detection cavity is communicated with the fourth detection hole (1011), the middle detection cavity is communicated with the gas inlet channel (101), and the detection control valve (25) is configured to control the communication or disconnection of the middle detection cavity and the bottom detection cavity.
9. The gas circuit integrated device for single-cell pretreatment according to claim 1, wherein the number of the gas inlet channels (101) is at least two, the number of the gas inlet hole groups is the same as that of the gas inlet channels (101), the gas inlet channels (101) and the gas inlet hole groups are arranged in a one-to-one correspondence manner, the number of the gas exhaust channels (103) is at least two, the number of the gas exhaust hole groups is the same as that of the gas exhaust channels (103), and the gas exhaust channels (103) and the gas exhaust hole groups are arranged in a one-to-one correspondence manner.
10. The gas circuit integrated device for single-cell pretreatment according to claim 1, further comprising a circuit board (3), wherein the circuit board (3) is electrically connected to the plurality of control valves respectively.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2022117053A1 (en) * | 2020-12-02 | 2022-06-09 | Suzhou Singleron Biotechnologies Co., Ltd. | Reagent exchange methods, devices, and systems |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
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| WO2022117053A1 (en) * | 2020-12-02 | 2022-06-09 | Suzhou Singleron Biotechnologies Co., Ltd. | Reagent exchange methods, devices, and systems |
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