CN213924834U

CN213924834U - Be applied to equipment of quantitative fluorochrome of RNA

Info

Publication number: CN213924834U
Application number: CN202023000460.4U
Authority: CN
Inventors: 汪妍妍; 张大生
Original assignee: Naying Shanghai Biotechnology Co ltd
Current assignee: Naying Shanghai Biotechnology Co ltd
Priority date: 2020-12-14
Filing date: 2020-12-14
Publication date: 2021-08-10
Anticipated expiration: 2030-12-14

Abstract

The utility model relates to the technical field of biological detection, in particular to a fluorescent dye device applied to RNA quantification; including box and case lid, the outside of box and case lid all is equipped with the heat preservation, be provided with the foam-rubber cushion in the instrument district, a plurality of holes of placing have been seted up on the foam-rubber cushion, place downthehole adsorption column, swab, centrifuging tube, pipettor, reagent bottle and EP pipe of placing, the electronic scale has been placed in the first workspace, the homogeneity appearance has been placed in the second workspace, centrifuge has been placed in the third workspace, the observation board has been placed in the fourth workspace, the observation groove has been opened on the surface of observation board, the rigid coupling has the chromatography membrane in the observation groove, the four corners position in observation groove all is equipped with fluorescence indicating groove, disposal bag and battery have been placed in the storage area. The utility model discloses a set up instrument district, centrifugation district and accomodate the district, can accomodate multiple instrument in the box, convenient to use, the function is various, and the practicality is good.

Description

Be applied to equipment of quantitative fluorochrome of RNA

Technical Field

The utility model relates to a biological detection technical field, in particular to be applied to equipment of RNA quantitative fluorochrome.

Background

Ribonucleic acid (abbreviated as RNA, RibonucleicAcid), a genetic information carrier present in biological cells and in parts of viruses, viroids. RNA is a long chain molecule formed by the condensation of ribonucleotides via phosphodiester bonds. One ribonucleotide molecule consists of a phosphate, a ribose and a base. RNA has 4 kinds of bases, namely A adenine, G guanine, C cytosine, U uracil, wherein U (uracil) replaces T in DNA.

When extracting and demarcating the operation, often need multiple instrument to test, current equipment of being applied to RNA ration's fluorescent dye does not have the function of accomodating, and the operation is inconvenient, and function singleness practicality is poor.

To this end, a device for fluorescent dyes for RNA quantification is proposed.

SUMMERY OF THE UTILITY MODEL

An object of the utility model is to provide an equipment for be applied to RNA quantitative fluorochrome to solve the problem that proposes in the above-mentioned technique.

In order to achieve the above purpose, the technical solution of the present invention is as follows:

the utility model provides an equipment for be applied to quantitative fluorochrome of RNA, includes box and case lid, the one end of case lid is passed through the hinge rotation with the top of box and is connected, and the buckle is connected between the other end and the box, the outside of box and case lid all is equipped with the heat preservation, inside first vertical baffle and the vertical baffle of second of being equipped with of box, first vertical baffle and the vertical baffle of second separate into instrument district, centrifugation district and storage area with box inside, be provided with the foam-rubber cushion in the instrument district, set up a plurality of holes of placing on the foam-rubber cushion, place adsorption column, swab, centrifuging tube, pipettor, reagent bottle and EP pipe in the placing hole, be provided with first transverse baffle, second transverse baffle and third transverse baffle in the centrifugation district, first transverse baffle, second transverse baffle and third transverse baffle separate into first workspace with the centrifugation district inside, The multifunctional garbage bag storage device comprises a second working area, a third working area and a fourth working area, an electronic scale is placed in the first working area, a homogenizer is placed in the second working area, a centrifugal machine is placed in the third working area, an observation plate is placed in the fourth working area, an observation groove is formed in the surface of the observation plate, a chromatographic membrane is fixedly connected in the observation groove, fluorescent indication grooves are formed in the four corners of the observation groove, and a garbage bag and a storage battery are placed in the storage area.

Specifically, horseradish peroxidase is arranged in the fluorescent indicating groove.

Specifically, the reagent bottles comprise a Hybridization Buffer reagent bottle, a Wash Buffer reagent bottle, an RNA reverse transcription reagent bottle, a pirocin reagent bottle and a real-time quantitative PCR reagent bottle.

Specifically, the EP tube comprises a spinning Buffer tube, a nucleic lysine Buffer tube, a streptavidin-labeled magnetic bead tube, a Protease K tube, a PMSF solution tube, an RNase A tube, an RNase H tube and an oligonucleotide random primer tube.

Specifically, the reagent bottles and the EP tubes are marked with numbers.

Specifically, the four corners of the bottom of the box body are provided with universal wheels, the side face of the box body is provided with a supporting plate, and a handle is arranged on the supporting plate.

Specifically, a sealing ring is further installed at the joint of the box body and the box cover.

The utility model has the advantages that: the utility model discloses a set up instrument district, centrifugation district and accomodate the district, can accomodate multiple instrument in the box, convenient to use, the function is various, and the practicality is good.

Drawings

Fig. 1 is a schematic structural diagram of an embodiment of the present invention;

fig. 2 is a top view of the case according to the embodiment of the present invention.

Reference numerals: the device comprises a box body 1, a box cover 2, a handle 3, a supporting plate 4, universal wheels 5, a sealing ring 6, a first vertical partition plate 7, a second vertical partition plate 8, a first transverse partition plate 9, a second transverse partition plate 10, a third transverse partition plate 11, a spongy cushion 12, a placing hole 13, a tool area 14, an electronic scale 15, a homogenizer 16, a centrifuge 17, an observing plate 18, an observing groove 19, a fluorescence indicating groove 20, a containing area 21, a garbage bag 22 and a storage battery 23.

Detailed Description

The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.

Referring to the attached drawings 1-2, a be applied to quantitative fluorescent dye's of RNA equipment, including box 1 and case lid 2, the one end of case lid 2 is passed through the hinge rotation with the top of box 1 and is connected, and the buckle is connected between the other end and the box 1, the outside of box 1 and case lid 2 all is equipped with the heat preservation, inside first vertical baffle 7 and the vertical baffle 8 of second of being equipped with of box 1, first vertical baffle 7 and the vertical baffle 8 of second separate into instrument district 14, centrifugal district and containing area 21 with box 1 is inside, be provided with foam-rubber cushion 12 in the instrument district 14, a plurality of placing holes 13 have been seted up on the foam-rubber cushion 12, place adsorption column, swab, centrifuging tube, pipettor, reagent bottle and EP pipe in the placing hole 13, be provided with first transverse baffle 9, second transverse baffle 10 and third transverse baffle 11 in the centrifugal district, first transverse baffle 9, The centrifugal area is internally divided into a first working area, a second working area, a third working area and a fourth working area by a second transverse partition plate 10 and a third transverse partition plate 11, an electronic scale 15 is placed in the first working area, a homogenizer 16 is placed in the second working area, a centrifugal machine 17 is placed in the third working area, an observation plate 18 is placed in the fourth working area, an observation groove 19 is formed in the surface of the observation plate, a chromatographic membrane is fixedly connected in the observation groove 19, fluorescent indication grooves 20 are formed in four corners of the observation groove 19, and a garbage bag 22 and a storage battery 23 are placed in the storage area 21; the utility model discloses a set up instrument district 14, centrifugation district and accomodate district 21, can accomodate multiple instrument in box 1, convenient to use, the practicality is good.

Furthermore, the utility model relates to a battery 23 for provide the electric energy to electrical components, the operation of being convenient for.

Specifically, horseradish peroxidase is arranged inside the fluorescent indicating groove 20; through setting up observation board 18, chromatographic membrane, observation groove 19 and fluorescence indication groove 20, can carry out the chromatography to the cell, place horseradish peroxidase in fluorescence indication groove 20's inside, react under the catalysis of horseradish peroxidase and give out light, produce the signal of high strength, and the luminous signal is permanent, even if can also observe single cell.

Specifically, reagent bottle and EP pipe superscript have the serial number, are convenient for look for reagent bottle and EP pipe, convenient to use.

Specifically, all be equipped with universal wheel 5 in the bottom four corners position of box 1, the side of box 1 is equipped with backup pad 4, be equipped with handle 3 in the backup pad 4, be convenient for remove.

Specifically, the sealing ring 6 is further installed at the joint of the box body 1 and the box cover 2, so that the use is convenient.

Further, the utility model relates to an inside top surface of case lid 2 still is equipped with the ice bag, and wherein, be equipped with the first face of pasting on the ice bag, be equipped with on the inside top surface of case lid 2 and paste the face with the first assorted second of pasting, be convenient for install, dismantle and change the ice bag, guaranteed in the transportation, RNA reverse transcription reagent and real-time quantitative PCR reagent are in the low temperature state all the time, can not be deactivated in the transportation.

Finally, although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that the present invention can be modified or replaced by other means without departing from the spirit and scope of the present invention, which should be construed as limited only by the appended claims.

Claims

1. The utility model provides an equipment for be applied to quantitative fluorochrome of RNA, includes box and case lid, its characterized in that, the one end of case lid is passed through the hinge rotation with the top of box and is connected, and the buckle is connected between the other end and the box, the outside of box and case lid all is equipped with the heat preservation, inside first vertical baffle and the vertical baffle of second of being equipped with of box, first vertical baffle and the vertical baffle of second separate into instrument district, centrifugation district and storage area with box inside, be provided with the foam-rubber cushion in the instrument district, set up a plurality of holes of placing on the foam-rubber cushion, place adsorption column, swab, centrifuging tube, pipettor, reagent bottle and EP pipe in the placement hole, be provided with first transverse baffle, second transverse baffle and third transverse baffle in the centrifugation district, first transverse baffle, second transverse baffle and third transverse baffle separate into first workspace with the centrifugation district inside, The multifunctional garbage bag storage device comprises a second working area, a third working area and a fourth working area, an electronic scale is placed in the first working area, a homogenizer is placed in the second working area, a centrifugal machine is placed in the third working area, an observation plate is placed in the fourth working area, an observation groove is formed in the surface of the observation plate, a chromatographic membrane is fixedly connected in the observation groove, fluorescent indication grooves are formed in the four corners of the observation groove, and a garbage bag and a storage battery are placed in the storage area.

2. The apparatus of claim 1, wherein the fluorescent indicator groove is provided with horseradish peroxidase.

3. The apparatus of claim 1, wherein the reagent bottles comprise Hybridization Buffer reagent bottles, Wash Buffer reagent bottles, RNA reverse transcription reagent bottles, pirocin reagent bottles and real-time quantitative PCR reagent bottles.

4. The apparatus of claim 1, wherein the EP tube comprises a spinning Buffer tube, a nucleic lysine Buffer tube, a streptavidin-labeled magnetic bead tube, a Protease K tube, a PMSF solution tube, an RNase A tube, an RNase H tube, and an oligonucleotide random primer tube.

5. The apparatus of claim 1, wherein the reagent bottle and the EP tube are numbered.

6. The apparatus of claim 1, wherein the bottom of the case is provided with universal wheels at four corners, and the side of the case is provided with a support plate, and the support plate is provided with a handle.

7. The apparatus of claim 1, wherein a sealing ring is further installed at the joint of the box body and the box cover.