CN213924824U - Extraction element and check out test set thereof - Google Patents

Abstract

An extraction device and detection equipment thereof, wherein the extraction device comprises a shell, a liquid supply cavity for storing liquid and a waste liquid cavity for collecting waste liquid are arranged in the shell; the liquid supply cavity is provided with a liquid adding port for adding liquid into the cavity, the liquid supply cavity is also provided with an air inlet, and the waste liquid cavity is provided with an exhaust hole; an adsorption cavity for enriching a target object is arranged in the shell, an adsorption carrier is arranged in the adsorption cavity, the liquid supply cavity is communicated to the adsorption cavity through a liquid supply channel, and the adsorption cavity is communicated to the waste liquid cavity through a waste liquid channel; the valve body is used for opening or closing the liquid supply channel and the waste liquid channel, a mounting hole for mounting the valve body is formed in the shell, and the valve body penetrates through the mounting hole and is inserted into the corresponding channel. Through integrated confession sap cavity, waste liquid chamber and each passageway in the casing, form the integral type structure, realize drawing, the waste liquid recovery of target object, need not to carry out complicated pipe connection in the casing outside, effectively improve the convenience of operation.

Description

Extraction element and check out test set thereof

Technical Field

The invention relates to the technical field of biological detection, in particular to an extraction device and detection equipment thereof.

Background

With the progress and development of basic medicine and clinical medicine, the diagnosis of genetic diseases, the diagnosis of epidemics and the concomitant diagnosis of personalized medicine using molecular biology are increasingly applied clinically. Currently, the major technical means of molecular biological analysis include qPCR, first-generation sequencing and second-generation sequencing. The implementation of the technical schemes requires a complete laboratory and a related series of instruments and equipment which are constructed and finished according to the requirements of the specifications. It also requires a trained professional and can take results after several days or even one to two weeks. All of these factors greatly limit the clinical applications of molecular diagnostics.

Therefore, the gene detection all-in-one machine appears in the market, clinical samples can be directly put into consumables, and the functions of clinical sample and nucleic acid extraction, PCR amplification, probe hybridization, signal detection, report printing and the like are realized in one cassette. One host drives the consumable card box to detect, and dependence of molecular detection on large laboratories, instruments and technicians is eliminated. These equipment mainly expand work around the consumptive material, utilize special structural design in the consumptive material card box and pre-buried reagent, realize from the sample processing to the multiple functions of signal detection.

However, the existing cartridges are complex in design, high in production cost, directly high in price, and not suitable for clinical application, especially for the primary clinical and consumer-grade gene detection markets.

SUMMERY OF THE UTILITY MODEL

The utility model provides a extraction element and check out test set thereof.

According to a first aspect, an embodiment provides an extraction device, comprising a housing, wherein a liquid supply cavity for storing liquid and a waste liquid cavity for collecting waste liquid are arranged in the housing;

the liquid supply cavity is provided with a liquid adding port for adding liquid into the cavity, the liquid supply cavity is also provided with an air inlet, and the waste liquid cavity is provided with an air outlet;

an adsorption cavity for enriching a target object is arranged in the shell, an adsorption carrier is arranged in the adsorption cavity, the liquid supply cavity is communicated to the adsorption cavity through a liquid supply channel, and the adsorption cavity is communicated to the waste liquid cavity through a waste liquid channel;

the valve body is used for opening or closing the liquid supply channel and the waste liquid channel, the shell is provided with a mounting hole for mounting the valve body, and the valve body penetrates through the mounting hole and is inserted into the corresponding channel.

The device will supply liquid chamber, waste liquid chamber, absorption chamber and each fluid channel integration in an organic whole, show the convenience that improves the operation, need not to realize supplying liquid, flowing back through external pipeline, external confession liquid container, external waste liquid container, the valve body plays the effect that the control corresponds the passageway break-make, valve body simple structure, easily manufacturing. Exhaust through the exhaust hole to the waste liquid chamber to control valve body opens the confession liquid channel that corresponds the confession liquid chamber, can supply the liquid supply in confession liquid chamber to adsorbing the chamber, and the adsorption carrier in the absorption chamber realizes the enrichment to the target object, and after the absorption, the waste liquid that leaves then flows out and adsorbs the chamber, gets into the waste liquid chamber through the waste liquid channel, realizes the collection to the waste liquid. This integral type box body structure has effectively integrated the function of stock solution, confession liquid, absorption target object, collection waste liquid, elution target object, realizes the absorption and the elution to the target object in the integral type device, and application method is simple, promotes the convenience of operation. The liquid in the liquid supply cavity can flow into the adsorption cavity by blowing air into the liquid supply cavity or exhausting air from the waste liquid cavity.

Optionally, the liquid supply cavity comprises a sample cavity for storing sample liquid, a washing liquid cavity for storing washing liquid, and an elution liquid cavity for storing elution liquid; the liquid supply channel comprises a sample injection channel, a washing liquid channel and an elution channel; the liquid outlet hole of the sample cavity is communicated to the adsorption cavity through a sample feeding channel, the liquid outlet hole of the eluent cavity is communicated to the adsorption cavity through a lotion channel, and the liquid outlet hole of the eluent cavity is communicated to the adsorption cavity through an elution channel. The liquid in the sample cavity or the washing liquid cavity can flow into the adsorption cavity by exhausting the waste liquid cavity; the sample chamber or the wash solution chamber may also be blown such that the liquid in the sample chamber or the wash solution chamber flows into the adsorption chamber. Can blow through the inlet port to the eluant chamber for the eluant in the eluant chamber flows into the absorption chamber, and then makes the eluant elute the target object of enrichment on with the adsorption film, and the eluant can get into each reaction hole through liquid outlet channel. Specifically, the adsorption cavity is communicated to the reaction holes through the liquid outlet channel, so that the eluent containing the target enters the corresponding reaction holes. Power equipment such as a vacuum pump and the like can be externally connected to the air inlet hole of the sample cavity or the washing liquid cavity, the corresponding liquid supply channel is opened through the control valve body, and air is blown into the cavity through the externally connected power equipment, so that liquid in the cavity flows and enters the adsorption cavity. The air inlet hole of the sample cavity or the washing liquid cavity can be naturally communicated with the atmospheric environment, the corresponding liquid supply channel is opened through the control valve body, the exhaust hole of the waste liquid cavity is externally connected to the power equipment, the waste liquid cavity is exhausted through the power equipment, and liquid in the liquid supply cavity flows into the adsorption cavity. The number of the sample cavity, the washing liquid cavity and the eluent cavity can be 1, 2 or more, the sample cavity, the washing liquid cavity and the eluent cavity can be designed as required, each cavity corresponds to one channel, a valve body for controlling the on-off of each channel is arranged on each channel, and the corresponding channel can be opened as required, so that liquid in the sample cavity, the washing liquid cavity and the eluent cavity flows into the adsorption cavity.

Optionally, an isolation cavity is arranged in the shell, the isolation cavity is communicated with an exhaust hole of the waste liquid cavity, a first connecting portion capable of communicating the cavity to power equipment is arranged on the outer wall of the isolation cavity, a transition cavity is further arranged in the shell, the transition cavity is communicated with an elution air inlet of the elution liquid cavity, and a second connecting portion capable of being communicated to the power equipment is arranged on the outer wall of the transition cavity. The isolation cavity can prevent waste liquid in the waste liquid cavity from flowing back into the liquid supply cavities such as the sample cavity and the liquid washing cavity. The transition cavity has certain cushioning effect to the air current, avoids power equipment's atmospheric pressure too big, causes the liquid in the eluant chamber because of the velocity of flow is too fast, causes the damage to the adsorption carrier. Certainly, also can not design isolation chamber, transition chamber, directly set up the first connecting portion that can feed through to waste liquid chamber and can external power equipment at waste liquid chamber's outer wall, directly set up the second connecting portion that can feed through to eluent chamber and can external power equipment at eluent chamber's outer wall, waste liquid chamber and the position that first connecting portion are connected, the position that eluent chamber and second connecting portion are connected can not contact liquid.

Optionally, the sample cavity and the washing liquid cavity are provided with air inlets which can be communicated to the external environment, and after the first connecting part is externally connected with power equipment, the air inlets of the sample cavity or the washing liquid cavity play a role of air intake when air is exhausted, so that liquid in the liquid supply channel and the waste liquid channel can flow normally.

Optionally, the first connecting portion and the second connecting portion are respectively located on the isolation cavity and the transition cavity towards the outer wall of the adsorption cavity. The structure is more compact, and position interference caused by the first connecting part and the second connecting part is avoided. If the first connecting part and the second connecting part are arranged on other outer walls of the isolation cavity and the transition cavity, the whole shell is inconvenient to operate when being placed into the heating chamber of the detection device due to position interference.

Optionally, the shell is provided with a plurality of reaction holes capable of containing reaction liquid, and the adsorption cavity is communicated with each reaction hole through a liquid outlet channel. The reaction hole is provided with a through hole communicated with the liquid outlet channel, so that liquid in the liquid outlet channel can flow into the reaction hole, and the top of the reaction hole is open, so that the liquid in the liquid outlet channel can smoothly circulate.

Optionally, the reaction holes are located on the same horizontal plane, the channels between the adsorption cavity and the reaction holes are equidistant, and the equidistant channels can enable the liquid amount flowing into the reaction holes to be almost consistent, so that the subsequent detection is facilitated.

Optionally, the liquid outlet channel includes a primary channel, a secondary channel, and a tertiary channel, which are sequentially communicated, the primary channel is communicated to the adsorption cavity, the secondary channels are connected in parallel, the tertiary channels are connected in parallel, the reaction holes are communicated to the tertiary channels, the lengths of the secondary channels are equal, the lengths of the tertiary channels are equal, the primary channel is communicated to a plurality of next parallel primary channels, and each tertiary channel is communicated with one reaction hole. The graded channels can make the internal liquid flow to the next-stage channel uniformly and finally flow into each reaction hole uniformly. In one embodiment, each secondary channel is connected to five tertiary channels, each tertiary channel is connected to one reaction hole, and a total of two tertiary channels are provided in the housing, so that a total of ten reaction holes are provided in the housing.

Optionally, the liquid outlet channel includes a first-stage channel, a second-stage channel, a third-stage channel, a fourth-stage channel, and a fifth-stage channel, which are sequentially connected, the first-stage channel is communicated to the adsorption cavity, the second-stage channels are connected in parallel, the third-stage channels are connected in parallel, the fourth-stage channels are connected in parallel, the fifth-stage channels are connected in parallel, the reaction holes are communicated to the fifth-stage channel, the lengths of the second-stage channels are equal, the lengths of the third-stage channels are equal, the lengths of the fourth-stage channels are equal, the lengths of the fifth-stage channels are equal, the previous-stage channel is communicated to a plurality of next-stage channels, and each fifth-stage channel is communicated with one reaction hole. In one embodiment, each four-stage channel is connected with four parallel five-stage channels, each five-stage channel is connected with one reaction hole, 8 four-stage channels are arranged in the shell, and 32 reaction holes are formed in the shell.

Optionally, the number of stages of the liquid outlet channel is not limited, and the last stage of channel is in one-to-one correspondence communication with each reaction hole.

Alternatively, the adsorbent support includes, but is not limited to, a glass fiber filter membrane. The adsorption carrier can be in other forms such as a granular form, and the adsorption and elution of the adsorption carrier on the target object are realized by introducing solutions with different pH values and ionic strengths into the adsorption cavity, so that the extraction of the target objects such as nucleic acid, protein and the like is realized.

Optionally, the target includes, but is not limited to, at least one of nucleic acid, protein, and specifically may be at least one of DNA, RNA, protein, and the like.

Optionally, the housing includes a top plate that can be closed to an opening of the liquid supply chamber. The design of roof helps improving the convenience of liquid feeding, and when needing the liquid feeding, takes off the roof, and the opening that supplies the liquid chamber is great, can easily add the solution of preparing and correspond each and supply the liquid chamber, and the phenomenon that liquid spills to the chamber outside is difficult for appearing.

Optionally, still be equipped with the handle on the casing, the staff of being convenient for holds the handle, puts into check out test set's installation room with the casing, and convenient operation also avoids other positions of hand contact casing, and then improves the risk of pollution.

Optionally, the housing includes an upper housing and a lower housing, the liquid supply chamber is disposed in the upper housing, and the waste liquid chamber is disposed in the lower housing. Under the action of gravity, liquid in the waste liquid cavity cannot flow back into the waste liquid channel, so that pollution is effectively avoided, and the channels can be designed on the lower part of the upper shell and/or the upper part of the lower shell, so that processing of the channels is facilitated.

Optionally, a clamping and stopping structure is arranged on the housing, and when the housing slides into the heating chamber of the host, the clamping and stopping structure clamps and stops the housing. It is common to manually push the housing into the heating chamber of the host machine.

Optionally, the card stop structure is located at a bottom of the lower housing. When the shell enters the heating chamber of the detection device for a certain depth, the clamping structure at the bottom clamps the shell. The blocking structure can also be positioned at the upper part of the upper shell, a blocking structure is arranged in the detection equipment, and after the shell enters a heating chamber of the detection equipment to a certain depth, the blocking structure is contacted with the blocking structure, so that the shell is blocked. And when the shell enters a heating chamber of the detection equipment to a certain depth, the blocking structure is contacted with the outer wall of the liquid supply cavity, so that the shell is blocked.

Optionally, the card stops the structure and includes that first protruding stupefied, the second that is close to or is located casing bottom edge is stupefied, and first protruding stupefied, the second is stupefied and is located same straight line, and first protruding stupefied is located the reaction zone below, and the second is stupefied and is higher than first protruding stupefied, forms contained angle shape recess between first protruding stupefied and the second protruding stupefied, and contained angle shape recess realizes the card of casing and stops.

Optionally, each of the liquid supply channel and the waste liquid channel is provided with the valve body, the valve body is provided with a liquid passing hole corresponding to the channel, the valve body is pressed down through equipment such as a motor, when the liquid passing hole is opposite to the channel, the channel is opened, and when the solid part of the valve body is opposite to the channel, the channel is closed. The valve body can realize the control of the flow of the channel and the on-off of the channel.

Optionally, the extraction device further comprises a cover plate capable of covering each reaction hole, and the cover plate is provided with a protrusion capable of plugging the reaction holes, so that liquid in the reaction holes can be prevented from being polluted.

Optionally, the extraction device further includes a protection plate located between the cover plate and the reaction hole, and the protection plate is provided with a hollow protection plug or a protection film corresponding to the reaction hole. The protective plug or membrane of the mesopores can prevent the liquid of the reaction pores from being contaminated.

According to a second aspect, an embodiment provides a detection apparatus comprising the extraction device of the first aspect.

Optionally, the detection apparatus further comprises a heating chamber for placing the extraction device. The heating chamber can be internally provided with a limiting structure for limiting the shell, for example, a concave track is arranged in the heating chamber, a convex edge corresponding to the concave track is arranged at the bottom of the shell, and the convex edge is placed in the concave track, so that the limitation on the shell can be realized.

According to the extraction element of above-mentioned embodiment, through integrated sample chamber, lotion chamber, eluant chamber, waste liquid chamber and each passageway in the casing, form the integral type structure, realize that extraction, the waste liquid of target object are retrieved, need not to carry out complicated pipe connection in the casing outside, effectively improve the convenience of operation. The valve body plays a role in controlling the on-off of fluid in each fluid channel, and is simple in structure and low in production cost.

Drawings

FIG. 1 is a schematic diagram of a front view of an embodiment of an extracting device;

FIG. 2 is a bottom view of FIG. 1;

FIG. 3 is a bottom view of FIG. 2;

FIG. 4 is a schematic perspective view of an embodiment of an extraction device;

FIG. 5 is a schematic perspective view of an embodiment of an extraction device;

fig. 6 is an enlarged view of the portion a of fig. 5.

FIG. 7 is a schematic perspective view of an embodiment of an extraction device;

FIG. 8 is a schematic front view of an upper housing of the extracting apparatus according to an embodiment;

FIG. 9 is a schematic diagram of the back structure of FIG. 8;

FIG. 10 is a bottom view of FIG. 8;

FIG. 11 is a perspective view of an upper housing of the extracting apparatus according to an embodiment;

FIG. 12 is an enlarged view of the portion B of FIG. 11;

FIG. 13 is a schematic view of an adsorption chamber according to an embodiment;

FIG. 14 is a schematic view showing a structure of a first valve body closing a passage;

FIG. 15 is a schematic view showing a structure of the first valve body opening the passage;

FIG. 16 is a perspective view of the first valve body;

FIG. 17 is a front view of the first valve body;

FIG. 18 is another schematic structural view of the first valve body;

FIG. 19 is a schematic diagram illustrating a front view of a lower housing of the extracting apparatus according to an embodiment;

FIG. 20 is a schematic view of the back structure of FIG. 19;

FIG. 21 is a bottom view of FIG. 19;

FIG. 22 is a perspective view of a lower housing of the extraction device according to an embodiment;

FIG. 23 is a schematic top view of an embodiment of an extractor;

FIG. 24 is a perspective view of a top plate according to an embodiment;

FIG. 25 is a schematic front view of a cover plate according to an embodiment;

FIG. 26 is a left side elevational view of FIG. 25;

FIG. 27 is a perspective view of a cover plate according to an embodiment;

fig. 28 is a front view schematically illustrating a protection plate according to an embodiment;

FIG. 29 is a right side elevational view of FIG. 28;

FIG. 30 is a perspective view of a protection plate according to an embodiment;

FIG. 31 is a schematic view of the housing inserted into the heating chamber according to an embodiment;

FIG. 32 is a schematic perspective view of an embodiment of a housing inserted into a heating chamber;

FIG. 33 is an enlarged view of portion C of FIG. 32;

FIG. 34 is a schematic front view of an extracting apparatus in another embodiment;

FIG. 35 is a schematic view of the back structure of FIG. 34;

FIG. 36 is a bottom view of FIG. 34;

FIG. 37 is a perspective view of an extracting device in another embodiment;

FIG. 38 is a schematic perspective view of an extraction device in another embodiment;

FIG. 39 is a front view of the upper housing of the extracting apparatus in another embodiment;

FIG. 40 is a schematic view of the back structure of FIG. 39;

FIG. 41 is a bottom view of FIG. 39;

fig. 42 is a perspective view of the upper housing of the extraction device in another embodiment.

Description of reference numerals:

description of numbering:

1. a first valve body; 101. a liquid passing hole; 2. a second valve body; 3. a third valve body; 4. a fourth valve body; 5. a fifth valve body; 6. A sixth valve body; 7. a housing; 71. an upper housing; 72. a lower housing; 8. a sample introduction channel; 9. a first wash solution channel; 10. a second wash solution channel; 11. an elution channel; 12. a waste liquid channel; 13. a liquid outlet channel; 131. a primary channel; 132. a secondary channel; 133. a tertiary channel; 134. a four-stage channel; 135. a five-stage channel; 14. a sample chamber, 141, a liquid outlet; 15. a first wash chamber; 16. a second wash chamber; 17. an eluate chamber; 171. an elution air inlet hole; 18. a waste fluid chamber; 19. a first through hole; 20. a second through hole; 21. a third through hole; 23. a gas passing through hole; 24. a reaction zone; 25. a reaction well; 26. a reagent well; 27. A handle; 28. a first connection portion; 29. a second connecting portion; 30. adsorbing a carrier; 31. an adsorption chamber; 33. a support plate; 34. An isolation chamber; 35. a top plate; 36. a cover plate; 361. a first protrusion; 362. a second protrusion; 37. a protection plate; 371. a protective plug; 372. a protective hole; 701. mounting holes; 38. a detection device; 39. a transition chamber; 42. a card stop structure; 421. a first ridge; 422. a second ridge; 423. a groove; 43. a chute; 44. a heating chamber.

Detailed Description

The present invention will be described in further detail with reference to the accompanying drawings. Wherein like elements in different embodiments are numbered with like associated elements. In the following description, numerous details are set forth in order to provide a better understanding of the present application. However, those skilled in the art will readily recognize that some of the features may be omitted or replaced with other elements, materials, methods in different instances. In some instances, certain operations related to the present application have not been shown or described in detail in order to avoid obscuring the core of the present application from excessive description, and it is not necessary for those skilled in the art to describe these operations in detail, so that they may be fully understood from the description in the specification and the general knowledge in the art.

Furthermore, the features, operations, or characteristics described in the specification may be combined in any suitable manner to form various embodiments. Also, the various steps or actions in the method descriptions may be transposed or transposed in order, as will be apparent to one of ordinary skill in the art. Thus, the various sequences in the specification and drawings are for the purpose of describing certain embodiments only and are not intended to imply a required sequence unless otherwise indicated where such sequence must be followed.

The numbering of the components as such, e.g., "first", "second", etc., is used herein only to distinguish the objects as described, and does not have any sequential or technical meaning. The term "connected" and "coupled" when used in this application, unless otherwise indicated, includes both direct and indirect connections (couplings).

Example 1

In this embodiment, as shown in fig. 1, an extraction apparatus is provided, which is mainly used for extracting nucleic acid and/or protein, and includes a housing 7, a sample cavity 14 for storing sample liquid, a washing liquid cavity for storing washing liquid, and an eluent cavity 17 for storing eluent are provided in the housing 7, an adsorption cavity 31 for adsorbing a target object is provided in the housing 7, and a waste liquid cavity 18 for collecting waste liquid is provided in the housing 7; the number of the sample cavity 14, the wash cavity, the eluent cavity 17 and the waste liquid cavity 18 is not limited, and can be 1, 2, 3 or more, in this embodiment, the number of the wash cavities is two, including the first wash cavity 15 and the second wash cavity 16, for storing different washing liquids.

Sample cavity 14, first lotion cavity 15, second lotion cavity 16, elution liquid cavity 17 are last to be equipped with the inlet that is used for injecting into liquid respectively, and specifically, the top in sample cavity 14, first lotion cavity 15, second lotion cavity 16, elution liquid cavity 17 is the opening form, and roof 35 can cover the open-top of closing to each cavity, seals each cavity.

As shown in fig. 9 and 13, an adsorption carrier 30 is disposed in the adsorption cavity 31, the sample cavity 14 is communicated with the adsorption cavity 31 through the sample introduction channel 8, the first wash liquid cavity 15 is communicated with the adsorption cavity 31 through the first wash liquid channel 9, the second wash liquid cavity 16 is communicated with the adsorption cavity 31 through the second wash liquid channel 10, the eluent cavity 17 is communicated with the adsorption cavity 31 through the elution channel 11, and the waste liquid cavity 18 is communicated with the adsorption cavity 31 through the waste liquid channel 12. So that each chamber can supply liquid to the adsorption chamber 31, and waste liquid in the adsorption chamber 31 can enter the waste liquid chamber through the waste liquid channel 12.

In one embodiment, the inner diameter of each fluid channel of the sample channel 8, the first wash channel 9, the second wash channel 10, the elution channel 11, the waste channel 12, the effluent channel 13, etc. may be about 1 mm. When the adsorption carrier 30 is an adsorption film, the diameter of the adsorption film may be 3-10mm, the thickness of the adsorption film is usually about 0.5mm, and a plurality of adsorption films may be placed in the adsorption cavity 31, for example, 3, 4 or 5 adsorption films may be placed to improve the adsorption efficiency and the loading capacity, and the adsorption film is usually highly hydrophilic. The bottom of the adsorption cavity 31 can be concave, which is convenient for placing the adsorption film. The height of the adsorption cavity 31 can be 2-3mm, and the adsorption film can be contained. The inner diameter of the adsorption cavity 31 may be slightly larger than the diameter of the adsorption film, so as to facilitate the placement of the adsorption film.

Sample chamber 14, first lotion chamber 15, second lotion chamber 16, eluant chamber 17, the passageway of adsorbing chamber 31 and each cavity of intercommunication is whole to be integrated in casing 7, need not external pipeline in order to input required solution, can realize the absorption and the elution to the target object in the integral type device, and realize the collection to the waste liquid, effectively simplify the operation, improve the convenient to use nature of the device, the use place is unrestricted, can go on in places such as hospital, clinic, need not to go on in special laboratory. The device has simple structure and low production cost, and is suitable for the primary clinical and consumption level gene detection markets.

In one embodiment, as shown in fig. 1, 2, 8, and 9, the housing 7 includes an upper housing 71 and a lower housing 72, the upper housing 71 and the lower housing 72 can be connected together by ultrasonic welding, snap-fit, adhesion, or other fixing connection, the sample chamber 14, the first wash chamber 15, the second wash chamber 16, the eluent chamber 17, and the adsorption chamber 31 are disposed in the upper housing 71, and the waste chamber 18 is disposed in the lower housing 72. This configuration facilitates the machining of the channels and through holes. Fig. 8 to 12 are schematic structural views of the upper housing 71, and fig. 19 to 22 are schematic structural views of the lower housing 72. The sample inlet channel 8, the first washing liquid channel 9, the second washing liquid channel 10, the elution channel 11, the waste liquid channel 12, the liquid outlet channel 13 and other fluid channels are arranged on the upper shell 71, so that the processing of the fluid channels on the upper shell 71 is facilitated. Waste chamber 18 is disposed within lower housing 72 to facilitate the collection of waste fluid. In one embodiment, as shown in fig. 2, the upper housing 71 may be located at the upper portion of the lower housing 72, such that each liquid supply chamber is located above the waste liquid chamber 18, forming a top-down fluid flow direction, facilitating the flow of liquid through the adsorption chamber 31 and finally into the waste liquid 18 through the waste liquid channel 12, and preventing the waste liquid in the waste liquid chamber 18 from flowing back to the waste liquid channel 12.

In an embodiment, as shown in fig. 23 and 24, the housing 7 includes a top plate 35 that can cover the opening portions of the sample chamber 14, the first wash chamber 15, the second wash chamber 16, and the eluent chamber 17, the first through hole 19, the second through hole 20, and the third through hole 21 are disposed on the top plate 35, and the first through hole 19, the second through hole 20, and the third through hole 21 are air inlets, so as to facilitate ventilation during air suction. In one embodiment, the first through hole 19, the second through hole 20, and the third through hole 21 may be installed with a gas-permeable, water-impermeable semi-permeable membrane, so as to reduce the contamination of the liquid in the chamber. The top plate 35 may be detachably connected to the upper case 71. The top plate 35 acts as a closed chamber. In a preferred embodiment, the top plate 35 can be covered on the top opening of each chamber by a snap-fit method, etc. to close each chamber. The top plate 35 may be an integral cover plate that covers the opening of each chamber in a uniform manner, the integral structure facilitates rapid manufacturing, and each portion may be injection molded and then formed by ultrasonic welding or dispensing. Roof 35 also can be the independent apron with each cavity one-to-one, and each roof 35 independently covers closes to the cavity opening that corresponds, and split type structure can the pollution of minimizing the cavity, when needing to pour into corresponding liquid into certain cavity, uncover the roof 25 of this cavity can, other cavities can not opened, and then avoid other cavities to cause the pollution because of exposing in the air. The top plate 35 may also be connected to the upper housing 71 by other means such as hinges.

In an embodiment, as shown in fig. 25, 26, and 27, the apparatus further includes a cover plate 36 that can be tightly covered to each reaction hole 25, the cover plate 36 is provided with a plurality of first protrusions 361 and second protrusions 362, the second protrusions 362 correspond to each reaction hole 25 and reagent hole 26, the upper housing 71 is correspondingly provided with concave holes, the first protrusions 361 are inserted into the corresponding concave holes, and the second protrusions 362 block the corresponding reaction holes 25, so as to prevent the liquid in the reaction holes 25 from being contaminated. The reagent hole is used for storing the liquid of the control group, the corresponding liquid can be added into the reagent hole during processing, and then the reagent hole is sealed, so that pollution is avoided. After the reaction is finished, the closed hole can be photographed, and then the image is analyzed to obtain a detection result.

In an embodiment, as shown in fig. 28, 29, and 30, the housing 7 further includes a protection plate 37 tightly covering each reaction hole 24, the protection plate 37 is provided with a hollow protection plug 371 for protecting the liquid in the reaction hole 25, the protection plug 371 is in one-to-one correspondence with the reaction holes, the hollow hole 372 of the protection plug 371 corresponds to the top opening of the reaction hole 25, when the protection plate 37 is tightly covered, one side of the protection plug 371 directly faces the reaction hole 25, one side edge of the protection plug 371 directly faces the reaction hole 25 is tightly abutted to the upper edge of the reaction hole 25, the other side of the protection plug 371 directly faces the cover plate 36, the second protrusion 362 is inserted into the corresponding hollow hole 372, and the length of the second protrusion 362 is smaller than the depth of the hollow hole 372, so that the second protrusion 362 does not contact with the liquid in the reaction hole 25, thereby avoiding contamination. The protection plugs 371 may be made of elastic materials such as rubber, and the second protrusions 362 of the cover plate 36 may be in interference fit with the corresponding hollow holes 372 of the protection plugs 371, so that the cover plate 36 has a good sealing effect while tightly covering the reaction holes 25, and does not contaminate the liquid in the reaction holes 25. The reagent wells of the control group can be designed to be closed during production without being covered and protected by the protection plate 37.

In one embodiment, as shown in fig. 4, a plurality of mounting holes 701 are provided on the housing 7, and each mounting hole 701 is provided with a valve body, and the valve body includes a first valve body 1 for controlling the sample feeding channel 8, a second valve body 2 for controlling the first washing liquid channel 9, a third valve body 3 for controlling the second washing liquid channel 10, a fourth valve body 4 for controlling the elution channel 11, a fifth valve body 5 for controlling the waste liquid channel 12, and a sixth valve body 6 for controlling the liquid outlet channel 13. Each valve body can be the valve body of silica gel material, and the valve body passes through interference fit's mode and installs to corresponding mounting hole 701, is equipped with at least one through-hole on the valve body, and when being equipped with a plurality of through-holes, each through-hole has certain spacing distance in the direction of pressing for the valve body can be under the power effect of external equipment such as lead screw motor, open or close the passageway at valve body place. The following description will take the first valve body 1 as an example, the structure of other valve bodies may be the same as or similar to that of the first valve body 1, as shown in fig. 14, 15, 16, and 17, the first valve body 1 is provided with 1 liquid passing hole 101, a solid structure is provided below the liquid passing hole 101, which can block the sample introduction channel 8, when injecting liquid into the sample cavity 14, the sample introduction channel 8 needs to be closed, the first valve body 1 is inserted into the sample introduction channel 8 through the mounting hole 71, and the solid part below the liquid passing hole 101 blocks the sample introduction channel 8, so that the sample introduction channel 8 is blocked and the liquid cannot flow; when liquid needs to be supplied to the adsorption cavity 31 through the sample introduction channel 8, the screw motor above the shell 7 presses the first valve body 1 downwards through the push rod until the liquid passing hole 101 on the first valve body 1 is opposite to the through hole of the sample introduction channel 8, the screw motor stops working, and liquid in the sample cavity 14 can enter the adsorption cavity 31 through the sample introduction channel 8 under the air suction effect of power equipment such as a vacuum pump. When liquid supply is not needed, the screw motor is started again, the first valve body 1 is pressed down again through the push rod, so that the solid part above the liquid passing hole 101 in the first valve body 1 blocks the sample feeding channel 8, and the sample feeding channel 8 is closed. In an embodiment, the liquid passing hole 101 of the first valve body 1 can be aligned with the sample introduction channel 8 or a solid part of the first valve body 1 blocks the sample introduction channel 8 by horizontally rotating the first valve body 1, so as to open or close the sample introduction channel 8.

In an embodiment, as shown in fig. 18, two liquid passing holes 101 may be formed in the first valve body 1, the two liquid passing holes 101 are longitudinally arranged, and a solid portion capable of blocking the sample channel 8 is formed between the two liquid passing holes 101, so that the first valve body 1 can open the sample channel 8 twice. The other valve bodies may also be identical or similar in structure to the aforementioned first valve body 1. The valve body can be longitudinally provided with 3 or more liquid passing holes 101, and a solid structure capable of blocking a channel is arranged between the liquid passing holes, so that the valve body can realize more opening and closing actions.

In one embodiment, as shown in fig. 1-13, a separation cavity 34 is provided in the housing, the separation cavity 34 is communicated with the waste liquid cavity 18, specifically, the separation cavity 34 can be communicated with the waste liquid cavity 18 through a gas through hole 23 at the bottom, a first connecting portion 28 which can be communicated with the power equipment is provided on the separation cavity 34, a transition cavity 39 is further provided in the housing 7, an outlet of the transition cavity 39 can be communicated with the eluent cavity 17 through an elution inlet hole 171, and a second connecting portion 29 which can be communicated with the power equipment is provided on the transition cavity 39. The segregation chamber 34 serves to prevent back-suction of liquid in the waste chamber 18 and direct contact of the motive gas with the liquid. The first connecting portion 28 and the second connecting portion 29 may be cylindrical structures, channels communicated with the corresponding chambers are arranged in the cylindrical structures, the power equipment may be a vacuum pump, and the like, and air pipes of the vacuum pump are connected to the first connecting portion 28 and/or the second connecting portion 29 to provide flowing power for liquid in the communicated chambers through air suction or air blowing. For example, when the vacuum pump pumps air to the isolation cavity 34 through the first connection portion 28, since the isolation cavity 34 is communicated to the waste liquid cavity 18 through the air through hole 23 at the bottom, a negative pressure is generated when the waste liquid cavity 18 is communicated, and further a negative pressure is generated in the adsorption cavity 31, if the sample introduction channel 8 is opened, then the sample liquid in the sample cavity 14 communicated with the sample introduction channel 8 will flow into the sample introduction channel 8 through the liquid outlet 141 at the bottom and further flow into the adsorption cavity 31 under the action of the negative pressure, the target object in the sample liquid will be adsorbed on the adsorption carrier 30, the non-target object and the solvent form a waste liquid, and the waste liquid flows into the waste liquid cavity 18 through the waste liquid channel 12, thereby collecting the waste liquid.

In fig. 8, the two chambers at the front sides of the first wash liquid chamber 15 and the second wash liquid chamber 16 are only arranged side by side with the isolation chamber 34 and the transition chamber 39 for the convenience of manufacturing. It is also possible to eliminate two chambers on the front side of the first wash chamber 15 and the second wash chamber 16, or to move the front side edges of the first wash chamber 15 and the second wash chamber 16 forward to keep them on the same straight line with the front side edges of the partition chamber 34 and the transition chamber 39, so as to facilitate manufacturing.

In an embodiment, as shown in fig. 1, one surface of the housing 7 on which each valve body is disposed is a front surface, the opposite other surface is a back surface, the isolation cavity 34 and the transition cavity 39 are located on the front surface of the housing 7 and protrude upward, and the first connection portion 28 and the second connection portion 29 are located on the isolation cavity 34 and the transition cavity 39, respectively, and face the outer wall of the adsorption cavity 31, so that the structure is more compact, the position interference is reduced, and the air pipe of the vacuum pump is conveniently connected to the first connection portion 28 and the second connection portion 29.

In one embodiment, as shown in fig. 8 and 11, the sample chamber 14, the first wash chamber 15, the second wash chamber 16, and the eluent chamber 17 in the housing 7 are adjacent in sequence, and are located on the front surface of the housing 7, and are disposed at one end of the housing 7, i.e., the liquid supply end, the isolation chamber 34 is adjacent to the sample chamber 14, and the transition chamber 39 is adjacent to the eluent chamber 17, so as to facilitate air suction or blowing.

In one embodiment, as shown in fig. 9, the adsorption chamber 31 is located at the center of the housing 7, so that each liquid supply chamber supplies the required liquid to the adsorption chamber 31.

In an embodiment, as shown in fig. 1 to 4, the casing 7 is further provided with a handle 27, the position of the handle 27 is not limited, and the handle is convenient for an operator to hold, and specifically, the handle can be located at one end close to the liquid supply cavity, in an embodiment, as shown in fig. 8, the main body of the casing 7 is in a rectangular structure, the sample cavity 14, the first wash cavity 15, the second wash cavity 16, and the eluent cavity 17 are located at one end of the casing 7 where the short edge is located, that is, the liquid supply end, the handle 27 can be arranged at the outer side of the liquid supply end of the casing 7, so that the operator can hold the handle 27, and put the whole extraction device into the corresponding position. The reaction zone 24 is close to the other end of the housing 7 opposite to the liquid supply end, and the operator holds the handle 27, and the end of the reaction zone 24 first enters the heating chamber for convenient operation.

In one embodiment, as shown in fig. 9, the housing 7 is provided with a plurality of reaction holes 25 for accommodating the reaction solution, and the reaction holes 25 are communicated to the adsorption cavity 31 through the liquid outlet channel 13. The reaction area 24 is the area where the reaction holes 25 are located. In an embodiment, the sample cavity 14, the first wash cavity 15, the second wash cavity 16, the eluent cavity 17 and other liquid supply cavities are disposed on one side of the casing 7 close to the handle 27, that is, a liquid supply side, the reaction area 24 is disposed on the other side of the casing 7, that is, a reaction side, the liquid supply side and the heating side are opposite sides of the casing 7, and the adsorption cavity 31 is located in the middle of the casing 7.

In one embodiment, as shown in fig. 9, a plurality of reagent holes 26 are provided on the housing 7, and the reagent holes 26 are not communicated with each channel and are used for adding control group reagents, such as a negative control group, a positive control group, and the like.

In one embodiment, as shown in FIG. 9, the reaction wells 25 and the reagent wells 26 are located on the same horizontal plane, and the channel distance between the adsorption chamber 31 and each reaction well 25 is equal, so that the amounts of reagents flowing into each reaction well 25 through the adsorption chamber 31 and the corresponding channel are close to each other, thereby reducing errors in subsequent detection results in each reaction well 25.

In an embodiment, as shown in fig. 8 and 9, the liquid outlet channel 13 includes a first-stage channel 131, a second-stage channel 132, and a third-stage channel 133, which are sequentially connected, the first-stage channel 131 is connected to the adsorption cavity 31, the second-stage channels 132 are connected in parallel, the third-stage channels 133 are connected in parallel, the reaction holes 25 are connected to the third-stage channels 133, the lengths of the second-stage channels 132 are equal, the lengths of the third-stage channels 133 are equal, the upper-stage channel is connected to a plurality of next-stage channels, and the third-stage channels are connected to a plurality of reaction holes. In one embodiment, as shown in fig. 8 and 9, the primary channel 131 is connected to two parallel secondary channels 132, each secondary channel 132 is connected to five parallel tertiary channels 133, each tertiary channel 133 is connected to one reaction hole 25, there are 10 reaction holes, the liquid in the adsorption cavity 31 can flow into each reaction hole 25 almost simultaneously, and the amount of liquid in each reaction hole 25 is almost equal. The device can be used for detecting SARS-CoV-2 virus, detecting various human somatic mutations (auxiliary diagnosis of cancer and medication accompanying diagnosis), screening deafness gene mutation, etc.

As shown in fig. 2, 6, 7, 31, 32, and 33, the housing 7 is provided with a locking structure 42, the locking structure 42 is disposed at the bottom of the housing 7, the locking structure 42 includes a first protruding edge 421 and a second protruding edge 422 near or at the bottom edge of the housing 7, the first protruding edge 421 and the second protruding edge 422 are in the same line, the first protruding edge 421 is located below the reaction area, and when the housing is placed in the heating chamber 44, the first protruding edge 421 first enters the heating chamber 44. The second protruding stupefied 422 is higher than the first protruding stupefied 421, forms contained angle shape recess 423 between first protruding stupefied 421 and the second protruding stupefied 422, and the relative both sides in casing 7 bottom are equipped with first protruding stupefied 421, the second protruding stupefied 422 of symmetry, and two first protruding stupefied 421 are the slide rail, are convenient for slide in check out test set's spout 43, and when casing 7 slided into check out test set 38's heating chamber 44, the second was stupefied 422 stopped casing 7 card. The included angle-shaped groove 423 formed between the first protruding edge 421 and the second protruding edge 422 may be an obtuse angle or a right angle, which is convenient for stopping, and enables the housing 7 to be accurately placed in the corresponding position of the heating chamber 44, thereby avoiding dislocation. The locking structure 42 may be of other structures, for example, a concave slide rail structure (not shown), and a bottom of the heating chamber 44 of the detection device 34 is correspondingly provided with a protruding rib matched with the slide rail structure, so that the housing 7 can be accurately slid into the corresponding position.

Example 2

As shown in fig. 34 to 42, in this embodiment, in addition to the difference in the number of reaction holes in the reaction region and the number of stages of the outlet channels 13, the other structures are the same as those of embodiment 1, a first-stage channel 131, a second-stage channel 132, a third-stage channel 133, a fourth-stage channel 134 and a fifth-stage channel 135 are arranged in the casing 7 and are sequentially communicated, the first-stage channel 131 is communicated with the adsorption cavity 31, the second-stage channels 132 are connected in parallel, the third-stage channels 133 are connected in parallel, the fourth-stage channels 134 are connected in parallel, the fifth-stage channels 135 are connected in parallel, the reaction holes 25 are communicated with the fifth-stage channels 135, the lengths of the second-stage channels 132 are equal, the lengths of the third-stage channels 133 are equal, the lengths of the fourth-stage channels 134 are equal, the lengths of the fifth-stage channels 135 are equal, the upper-stage channel is communicated with two parallel lower-stage channels, and each fourth-stage channel 134 is independently connected with four reaction holes 25 through four fifth-stage channels 135. The total number of the four-level channels is 8, the shell 7 has 32 holes, and the 32-hole device can be used for detecting intestinal flora and the like.

The material of the extraction device can be PP (polypropylene), PC (polycarbonate) and the like, and each part of the device can be manufactured by injection molding and other processes, and the whole device can be obtained by bonding, ultrasonic welding, heat sealing or clamping assembly of each part.

In an embodiment, there is provided a detection apparatus comprising the extraction device of any of the preceding embodiments, further comprising a heating chamber 44 for placing the extraction device.

In one embodiment, the target includes, but is not limited to, at least one of nucleic acid, protein, and specifically, at least one of DNA, RNA, protein molecule, and the like.

In a preferred embodiment, the adsorption carrier 30 may be a glass fiber membrane (also referred to as a GF membrane) commercially available from, for example, Lefeng Biotech, Inc. of Hangzhou, Qiagen, Germany, and the like. The GF film has the functions of adsorbing and eluting target substances such as DNA and the like under the conditions of different ionic strengths and pH values, and is washed by combining with washing liquid, thereby playing the role of purifying the target substances such as DNA and the like. The adsorption carrier 30 may be in other forms such as a granular form.

In one embodiment, the operation of the extraction device as shown in fig. 1-13 is as follows:

1. the extraction device is inserted into the control system and the already lysed sample is added to the sample chamber 14.

2. The control system controls the first valve body 1 and the fifth valve body 5 to be opened.

3. The vacuum pump sucks the waste liquid cavity 18 for 30s through the first connecting hole 28, so that the sample liquid enters the adsorption cavity 31, the adsorption carrier 30 in the adsorption cavity 31 adsorbs the target object, the remaining waste liquid enters the waste liquid cavity 18 through the waste liquid channel 12, and the nucleic acid is adsorbed on the adsorption carrier 30.

4. The first valve body 1 is closed and the second valve body 2 is opened.

5. The vacuum pump sucks the waste liquid chamber 18 through the first connection hole 28 for 30s to wash the adsorption carrier 30 for the first time.

6. The second valve body 2 is closed and the third valve body 3 is opened.

7. The vacuum pump evacuates the waste liquid chamber 18 through the first connection hole 28 for 2min to wash the adsorption carrier 30 a second time.

8. The third valve body 3 is closed and the fifth valve body 5 is closed.

9. The fourth valve body 4 is opened and the sixth valve body 6 is opened.

10. The vacuum pump pressurizes the eluent chamber 17 through the second connection hole 29, the eluent enters the adsorption chamber 31, the target on the adsorption carrier 30 is eluted, and the target enters the liquid outlet channel 13 along with the eluent and then enters each reaction hole 25 of the reaction area 24.

11. Each reaction hole is sealed by a mode of sticking a film, hot melting or pressing a cover downwards.

12. And circularly heating the chamber in the host where the reaction hole part is positioned by hot air or wall heat, and after the reaction is finished, analyzing the reaction result of each reaction hole by photographing and other modes for subsequent health condition evaluation and the like.

It is right to have used specific individual example above the utility model discloses expound, only be used for helping to understand the utility model discloses, not be used for the restriction the utility model discloses. To the technical field of the utility model technical personnel, the foundation the utility model discloses an idea can also be made a plurality of simple deductions, warp or replacement.

Claims (10)

1. The extraction device is characterized by comprising a shell, wherein a liquid supply cavity for storing liquid and a waste liquid cavity for collecting waste liquid are arranged in the shell;

the liquid supply cavity is provided with a liquid adding port for adding liquid into the cavity, the liquid supply cavity is also provided with an air inlet, and the waste liquid cavity is provided with an air outlet;

an adsorption cavity for enriching a target object is arranged in the shell, an adsorption carrier is arranged in the adsorption cavity, the liquid supply cavity is communicated to the adsorption cavity through a liquid supply channel, and the adsorption cavity is communicated to the waste liquid cavity through a waste liquid channel;

the valve body is used for opening or closing the liquid supply channel and the waste liquid channel, the shell is provided with a mounting hole for mounting the valve body, and the valve body penetrates through the mounting hole and is inserted into the corresponding channel.

2. The extraction apparatus as claimed in claim 1, wherein the liquid supply chamber comprises a sample chamber for storing a sample liquid, a wash liquid chamber for storing a wash liquid, and an eluent chamber for storing an eluent; the liquid supply channel comprises a sample injection channel, a washing liquid channel and an elution channel; the liquid outlet hole of the sample cavity is communicated to the adsorption cavity through a sample feeding channel, the liquid outlet hole of the eluent cavity is communicated to the adsorption cavity through a lotion channel, and the liquid outlet hole of the eluent cavity is communicated to the adsorption cavity through an elution channel.

3. The extraction apparatus as claimed in claim 2, wherein a separation chamber is provided in the housing, the separation chamber is connected to the exhaust hole of the waste liquid chamber, a first connection part for connecting the chamber to a power device is provided on an outer wall of the separation chamber, a transition chamber is further provided in the housing, the transition chamber is connected to the elution inlet hole of the elution liquid chamber, and a second connection part for connecting the chamber to the power device is provided on an outer wall of the transition chamber.

4. The extraction apparatus as claimed in claim 3, wherein the first and second connection portions are respectively located on the outer walls of the isolation and transition chambers toward the adsorption chamber.

5. The extraction apparatus as claimed in claim 1, wherein the housing has a plurality of reaction holes for receiving the reaction solution, and the adsorption chamber is connected to each reaction hole via a liquid outlet channel.

6. The extraction apparatus as claimed in claim 5, wherein the reaction wells are in the same horizontal plane, and the adsorption chamber is spaced from the reaction wells at the same distance.

7. The extraction apparatus as claimed in claim 6, wherein said liquid outlet channel comprises a primary channel, a secondary channel, and a tertiary channel, which are connected in sequence, said primary channel is connected to said adsorption chamber, said secondary channels are connected in parallel, said tertiary channels are connected in parallel, said reaction holes are connected to said tertiary channels, said secondary channels are of equal length, said tertiary channels are of equal length, and said primary channel is connected to a plurality of next channels;

or the liquid outlet channel comprises a first-stage channel, a second-stage channel, a third-stage channel, a fourth-stage channel and a fifth-stage channel which are sequentially communicated, the first-stage channel is communicated with the adsorption cavity, the second-stage channels are mutually connected in parallel, the third-stage channels are mutually connected in parallel, the fourth-stage channels are mutually connected in parallel, the fifth-stage channels are mutually connected in parallel, the reaction holes are communicated with the fifth-stage channel, the lengths of the second-stage channels are equal, the lengths of the third-stage channels are equal, the lengths of the fourth-stage channels are equal, the lengths of the fifth-stage channels are equal, and the upper-stage channel is communicated with a plurality of next-stage channels which are parallel.

8. The extraction apparatus as claimed in claim 1, wherein said adsorption carrier comprises a glass fiber filter membrane;

the target is at least one of nucleic acid and protein;

the shell comprises a top plate which can cover the opening part of the liquid supply cavity;

the shell is also provided with a handle;

the shell comprises an upper shell and a lower shell, the liquid supply cavity is arranged in the upper shell, and the waste liquid cavity is arranged in the lower shell;

the shell is provided with a clamping stop structure, and when the shell slides into a heating chamber of the host, the shell is clamped and stopped by the clamping stop structure;

the clamping stop structure is positioned at the bottom of the lower shell;

the clamping structure comprises a first convex edge and a second convex edge which are close to or positioned at the edge of the bottom of the shell, the first convex edge and the second convex edge are positioned on the same straight line, the first convex edge is positioned below the reaction zone, the second convex edge is higher than the first convex edge, and an included angle-shaped groove is formed between the first convex edge and the second convex edge;

the valve body is arranged on each liquid supply channel and each waste liquid channel, and the valve body is provided with a liquid passing hole corresponding to the channel;

the device also comprises a cover plate which can be tightly covered to each reaction hole, and the cover plate is provided with a bulge which can block the reaction holes;

the device also comprises a protection plate positioned between the cover plate and the reaction hole, and a hollow protection plug corresponding to the reaction hole is arranged on the protection plate.

9. A detection apparatus comprising the extraction device according to any one of claims 1 to 8.

10. The inspection apparatus of claim 9, further comprising a heating chamber for housing the extraction device.

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