CN213903323U

CN213903323U - Contact type image forming apparatus

Info

Publication number: CN213903323U
Application number: CN202022571384.6U
Authority: CN
Inventors: 张英豪; 奚岩
Original assignee: Eport Life Sciences Shanghai Co ltd
Current assignee: Eport Life Sciences Shanghai Co ltd
Priority date: 2020-11-09
Filing date: 2020-11-09
Publication date: 2021-08-06
Anticipated expiration: 2030-11-09

Abstract

The utility model discloses a contact type imaging device, which comprises a shell and a detector; a darkroom space is arranged in the shell; the detector is positioned in the shell; the detector comprises a pixel array, wherein the pixel array comprises a plurality of pixels distributed in an array, and the size of each pixel is any value between 25 micrometers and 150 micrometers; the biological sample film is attached to the surface of the detector and positioned above the pixel array, and the detector is used for converting the optical signal into an image of the detected sample. The pixel size in the pixel array in the detector is set between 25um-150um, so that the detector has high resolution, the electronic capacity and lighting efficiency of a single pixel are improved, the sensitivity and dynamic range of equipment are further improved, and images acquired by the equipment are clearer.

Description

Contact type image forming apparatus

Technical Field

The utility model relates to a biological sample detects technical field, in particular to contact imaging device.

Background

Western blot (WB for short) technique, namely protein imprinting technique, is one of the most commonly used techniques in biomedical research. Protein bands are distributed on the membrane of the Western blot sample, and the bands are usually displayed by a chemiluminescence method. There are two traditional collection methods for the luminous pattern: one is a film method in which a sample film is attached to an X-ray film and a chemiluminescent sample is exposed to light in a pattern on the film. Although the imaging quality is high by adopting the film method, the problem of complex operation procedures exists; another is a camera method, which is to directly photograph a sample in a darkroom with a CCD (charge coupled device) camera. However, the camera CCD is limited by factors such as distance and sensitivity, and the loss of optical signals is large, and long-time exposure is often required to obtain a sufficiently clear signal. Based on the defects of large volume, large occupied space, high cost, difficult carrying and the like of the two imaging methods, a new imaging method appears in the prior art, namely, a complementary metal oxide semiconductor chip (CMOS for short) is adopted to carry out contact type acquisition on a sample film. In this arrangement the CMOS detector is spaced from the sample film either directly or by a very thin transparent resin or glass film and is therefore also referred to as contact imaging.

There are two types of prior art CMOS detectors, one CMOS-APS referred to as passive pixel detectors and the other CMOS-PPS referred to as active pixel detectors. The CMOS-APS detector has the characteristics of low yield, more bad pixels, high cost, low filling factor, small photosensitive area and low imaging efficiency. The CMOS-PPS detector has the advantages of high yield, few bad pixels, low cost, high filling factor, larger photosensitive area and high imaging efficiency. In order to reduce production cost and improve imaging efficiency, a CMOS-PPS detector is generally used in a contact type imaging apparatus for collecting a biological sample membrane.

The pixel size of a CMOS-PPS detector has a direct impact on the performance of the imaging device. The pixel size is too small, and the light collection efficiency and the electron capacity of a single pixel of the detector are greatly reduced although the resolution is relatively high. The light acceptance efficiency and electron capacity are proportional to the area of the pixel. The efficiency of the lighting and the pixel electron capacity are directly related to the sensitivity and dynamic range of the device, respectively. Thus, by choosing a small pixel size, the sensitivity and dynamic range of the device are reduced and narrowed. In addition, the manufacturing cost of the detector is also improved, and the yield in the manufacturing process is also greatly reduced. If the pixel size is too large, the resolution of the image may be degraded although the light collection efficiency and the electron capacity are improved. The resulting image had a mosaic visible to the naked eye. Therefore, selecting the proper pixel size is critical to the contact imaging device used to acquire the WB sample image.

SUMMERY OF THE UTILITY MODEL

The to-be-solved technical problem of the utility model is that the too big imaging effect of pixel size is not good in order to overcome contact imaging device among the prior art, and the acceptance efficiency of pixel size undersize light is low defect, provides a contact imaging device.

The utility model discloses an above-mentioned technical problem is solved through following technical scheme:

a contact imaging apparatus, comprising:

a housing having a darkroom space therein;

a probe located within the housing; the detector comprises a pixel array, wherein the pixel array comprises a plurality of pixels distributed in an array, and the size of each pixel is any value between 25 micrometers and 150 micrometers; the biological sample film is attached to the surface of the detector and positioned above the pixel array, and the detector is used for converting the optical signal into an image of the detected sample.

Preferably, the housing includes a light-shielding cover and a base hinged to each other, the base has an accommodating cavity with an open top, the detector is located in the accommodating cavity and connected to a top wall of the base, and the pixel array is located at the opening.

Preferably, the detector further comprises a fiber panel clamped on the side wall of the opening.

Preferably, the optical fiber panel is located above the pixel array, and the optical fiber panel is attached to the pixel array.

Preferably, the optical fiber panel and the shading cover enclose the darkroom space.

Preferably, the biological sample film is attached to the optical fiber panel, and the biological sample film is located in the darkroom space.

Preferably, the side length of the detector is set between 60mm and 160 mm.

Preferably, a clamping groove is formed in the top wall of the base, and the detector is clamped with the base through the clamping groove.

Preferably, a soft filler is arranged in the clamping groove, and the soft filler is located between the side wall of the detector and the side wall of the clamping groove.

Preferably, the detector is a CMOS chip detector.

On the basis of the common knowledge in the field, the above preferred conditions can be combined at will to obtain the preferred embodiments of the present invention.

The utility model discloses an actively advance the effect and lie in: the utility model discloses a contact imaging device, the pixel size setting among the pixel array in the detector is between 25um-150um, both has high resolution ratio, has improved single pixel's electron capacity and daylighting efficiency again, and then has improved the sensitivity and the dynamic range of equipment, makes the image of equipment collection clearer simultaneously.

Drawings

Fig. 1 is a schematic cross-sectional view of a contact image forming apparatus according to a preferred embodiment of the present invention.

Fig. 2 is another schematic cross-sectional view of a contact image forming apparatus according to a preferred embodiment of the present invention.

Fig. 3 is a schematic external structural view of a contact image forming apparatus according to a preferred embodiment of the present invention.

Description of reference numerals:

housing 10

Light shielding cover 101

Base 102

Detector 20

Fiber optic faceplate 30

Biological sample membrane 40

Darkroom 50

Filler 60

Detailed Description

The present invention will be more clearly and completely described below by way of examples and with reference to the accompanying drawings, but the present invention is not limited thereto.

As shown in fig. 1 to 3, the present embodiment provides a contact type imaging apparatus including a housing 10 and a probe 20; the shell 10 is internally provided with a darkroom 50 space; the probe 20 is located within the housing 10; the detector 20 comprises a pixel array, wherein the pixel array comprises a plurality of pixels distributed in an array, and the size of each pixel is any value between 25 micrometers and 150 micrometers; the biological sample film 40 is attached to the surface of the detector 20 and located above the pixel array, and the detector 20 is used for converting the optical signal into an image of the detected sample.

The detector 20 provided in this embodiment is a CMOS chip detector 20. The pixel size in the pixel array in the detector 20 is set between 25um-150um, which not only has high resolution, but also improves the electronic capacity and lighting efficiency of a single pixel, thereby improving the sensitivity and dynamic range of the device and making the image collected by the device clearer.

Specifically, the housing 10 includes a light shielding cover 101 and a base 102 hinged to each other, and by hinging the light shielding cover 101 and the base 102, the light shielding cover 101 is convenient to open and close, and thus the taking of a sample and the acquisition of image information are convenient. The base 102 has a cavity with an open top, the detector 20 is located in the cavity and connected to the top wall of the base 102, and the pixel array is located at the open. The probe 20 is attached to the top of the base 102 such that the probe 20 is fixed relative to the base 102 to improve the performance of the imaging device.

In this embodiment, the detector 20 further includes a fiber panel 30, and the fiber panel 30 is clamped on the side wall of the opening. The fiber optic faceplate 30 is located above the pixel array, and the fiber optic faceplate 30 is attached to the pixel array. Since the fiber panel 30 has sufficient rigidity, the fiber panel 30 has small light loss, and has the characteristic of total reflection, no matter how thick the fiber panel 30 is, the imaging effect of the detector 20 is not affected. Therefore, a fiber optic panel 30 is attached above the pixel array, so that the fiber optic panel 30 does not affect the imaging effect while protecting the detector 20. The optical fiber panel 30 and the light shielding cover 101 enclose a darkroom 50. The biological sample membrane 40 is attached to the optical fiber panel 30, and the biological sample membrane 40 is located in the dark room 50. To ensure effective collection of the weak optical signal on the biological sample membrane 40 by the detector 20.

In the present embodiment, the side length of the detector 20 is set between 60mm and 160 mm. Since most of the biological sample film 40 has a size not smaller than 60mm, the side length of the detector 20 is set between 60mm and 160mm to ensure that the detector 20 can expose most of the sample film. Moreover, the size of the detector 20 is not too large to ensure the yield of the detector 20, and thus the cost of the imaging device is not too high.

Further, a clamping groove is formed in the top wall of the base 102, and the detector 20 is clamped with the base 102 through the clamping groove. Moreover, be provided with soft filler 60 in the joint inslot, soft filler 60 is located between the lateral wall of detector 20 and the lateral wall in joint groove, when guaranteeing the fastness that detector 20 is connected with the joint groove, prevents hard contact between detector 20 and the base 102, and this kind of soft filler 60 has waterproof and dirt-proof effect simultaneously.

Although specific embodiments of the present invention have been described above, it will be understood by those skilled in the art that this is by way of example only and that the scope of the invention is defined by the appended claims. Various changes and modifications to these embodiments may be made by those skilled in the art without departing from the spirit and the principles of the present invention, and these changes and modifications are all within the scope of the present invention.

Claims

1. A contact image forming apparatus, comprising:

a housing having a darkroom space therein;

2. The contact imaging device as in claim 1, wherein said housing includes a cover and a base hingedly attached to each other, said base having an open-topped receiving cavity, said detector being located in said receiving cavity and attached to a top wall of said base, said pixel array being located at said opening.

3. The contact imaging device as claimed in claim 2, wherein said detector further comprises a fiber optic faceplate, said fiber optic faceplate being snapped into a sidewall of said opening.

4. The contact imaging device of claim 3, wherein the fiber optic faceplate is positioned over the pixel array, the fiber optic faceplate being attached to the pixel array.

5. The contact imaging apparatus of claim 3, wherein the fiber optic faceplate and the shutter cover enclose the dark room space.

6. The contact imaging apparatus of claim 3, wherein said biological sample membrane is attached to said fiber optic faceplate, said biological sample membrane being located within said dark room space.

7. The contact imaging apparatus of claim 1, wherein the detector has a side length set between 60mm and 160 mm.

8. The contact type image forming apparatus as claimed in claim 2, wherein a clamping groove is provided on a top wall of the base, and the detector is clamped with the base through the clamping groove.

9. The contact imaging apparatus of claim 8, wherein a soft filler is disposed in the snap groove, the soft filler being located between a sidewall of the probe and a sidewall of the snap groove.

10. The contact imaging device as claimed in any one of claims 1-9 wherein said detector is a CMOS chip detector.