CN213769814U - Flow type antibody storage and use kit - Google Patents
Flow type antibody storage and use kit Download PDFInfo
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- CN213769814U CN213769814U CN202021957353.8U CN202021957353U CN213769814U CN 213769814 U CN213769814 U CN 213769814U CN 202021957353 U CN202021957353 U CN 202021957353U CN 213769814 U CN213769814 U CN 213769814U
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- streaming antibody
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Abstract
The utility model discloses a STREAMING antibody is deposited and is used kit, including the reagent lid, the underrun crease fixed hinge of reagent lid has the reagent box body, six cold-stored STREAMING antibody framves of stand alone type, every have all been filled to the inside of the cold-stored STREAMING antibody frame of stand alone type has acrylic polyol polymer refrigerant, and is three little STREAMING antibody storage hole that the equidistance was arranged is all seted up to the upper surface of the cold-stored STREAMING antibody frame of stand alone type. This STREAMING antibody is deposited and is used kit, through the cold-stored STREAMING antibody frame of six solitary independencies of inside setting, can take out alone, supplies a dyeing scheme independent utility, the cold-stored STREAMING antibody frame of independent standing sets up respectively and has a little STREAMING antibody deposit hole and big tributary formula antibody deposit hole, can place the STREAMING antibody of different specifications to make things convenient for the storage of STREAMING antibody and take safer convenient more, also easily formulate new color scheme and be used for the experiment.
Description
Technical Field
The utility model belongs to the technical field of STREAMING analysis detects, especially, relate to a kit is deposited and is used to STREAMING antibody.
Background
Flow cytometry is a modern cell analysis developed in the seventies, integrates computer technology, laser technology, liquid mechanics, cytochemistry and immunology, has the functions of analyzing and sorting cells, separates cells with different cell sizes and cell inner granularities by collecting forward scattered light and lateral scattered light, and realizes the rapid measurement of cell biological indicators such as cell surface antigens, cell factors and the like by identifying fluorescent markers with cell surface and cell inner specificity, but flow antibodies have higher requirements on storage and use, antibodies are easy to reduce in activity at normal temperature, binding specificity is reduced, fluorescent markers are easy to cause fluorescent quenching under the irradiation of natural light, detection signals are weakened, the accuracy of analysis results is finally influenced, and antibodies of a multicolor system are easy to be disordered in the using process to cause serious cross color during detection, the analysis of the final result is not facilitated, and a portable flow-type antibody kit which can control temperature and avoid light is needed, so that the flow-type antibody storage and use kit is provided for solving the problems.
SUMMERY OF THE UTILITY MODEL
The utility model provides a, aim at solving above-mentioned antibody that has polychrome system and easily be chaotic in the use, serious cross color when leading to detecting, be unfavorable for final result analysis's problem.
The utility model discloses a realize like this, a STREAMING antibody is deposited and is used kit, including the reagent lid, the underrun crease fixed hinge of reagent lid has the reagent box body, six cold-stored STREAMING antibody framves of stand alone type, every are placed to the inside of reagent box body the inside of cold-stored STREAMING antibody frame of stand alone type all is filled with many acrylic acid polyol polymer refrigerant, three little STREAMING antibody storage hole, one that the equidistance was arranged are all seted up to the upper surface of cold-stored STREAMING antibody frame of stand alone type the big tributary formula antibody storage hole that the equidistance was arranged is seted up to the upper surface of cold-stored STREAMING antibody frame of stand alone type, two little STREAMING antibody storage hole and big tributary formula antibody storage hole that the equidistance was arranged are seted up respectively to the upper surface of cold-stored STREAMING antibody frame of stand alone type.
The front surface of the reagent box cover is provided with fluorescence intensity and a fluorescence spectrum of common fluorescence names under each laser of the flow cytometer.
The positive fixedly connected with box of reagent box body, the left surface fixedly connected with son of reagent lid is buckled, the box is buckled mutually with the son.
Two sets of symmetrical supporting pads are arranged below the reagent box body, and each supporting pad is fixedly connected with the bottom surface of the reagent box body on the upper surface.
The reagent box cover is characterized in that a nameplate is fixedly connected to the upper surface of the reagent box cover, and a label is arranged on the front face of the nameplate.
The reagent box body is cuboid, and the reagent box body is a black shell.
Compared with the prior art, the beneficial effects of the utility model are that: set up six solitary cold-stored STREAMING antibody framves of stand alone type through reagent box body's inside, can take out alone when using, supply a dyeing scheme independent utility, the cold-stored STREAMING antibody of stand alone type puts up and sets up respectively that little STREAMING antibody deposits the hole and big tributary formula antibody deposits the hole, can place the STREAMING antibody of different specifications to make things convenient for the storage of STREAMING antibody and take safer convenient, also easily formulate new color matching scheme and be used for the experiment.
Drawings
Fig. 1 is a schematic perspective view of the reagent cartridge of the present invention;
FIG. 2 is a side view of the reagent box cover of the present invention in a closed state;
fig. 3 is a top view of the reagent box cover of the present invention.
In the figure: 1. a reagent box cover; 2. a freestanding cryopreserved flow antibody scaffold; 3. a small bypass antibody storage well; 4. a big branch flow type antibody storage hole; 5. a reagent cartridge body; 6. a fluorescence spectrum; 7. a female buckle; 8. a sub-buckle; 9. a support pad; 10. a nameplate; 11. and (4) a label.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more clearly understood, the present invention is further described in detail below with reference to the accompanying drawings and embodiments. It should be understood that the specific embodiments described herein are merely illustrative of the invention and are not intended to limit the invention.
In the description of the present invention, it is to be understood that the terms "length", "width", "upper", "lower", "front", "rear", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like indicate orientations or positional relationships based on those shown in the drawings, and are merely for convenience of description and simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and thus, should not be construed as limiting the present invention. In addition, in the description of the present invention, "a plurality" means two or more unless specifically limited otherwise.
Referring to fig. 1-3, a kit for storing and using a flow-type antibody comprises a reagent box cover 1, wherein a reagent box body 5 is fixedly hinged to the bottom surface of the reagent box cover 1 through a crease, six independent refrigeration flow-type antibody racks 2 are placed inside the reagent box body 5, a polyacrylic acid polyol polymer refrigerant is filled inside each independent refrigeration flow-type antibody rack 2, small branch-type antibody storage holes 3 which are arranged equidistantly are formed in the upper surfaces of the three independent refrigeration flow-type antibody racks 2, large branch-type antibody storage holes 4 which are arranged equidistantly are formed in the upper surface of one independent refrigeration flow-type antibody rack 2, and small branch-type antibody storage holes 3 and large branch-type antibody storage holes 4 which are arranged equidistantly are formed in the upper surfaces of the two independent refrigeration flow-type antibody racks 2.
In the utility model, the front of the reagent box cover 1 is provided with the fluorescence intensity of the common fluorescence name under each laser of the flow cytometer and a fluorescence map 6, and the fluorescence map 6 is marked with ultraviolet lasers 355nm and BUV395 fluorescence intensity 2; ultraviolet laser 405nm, V450 fluorescence intensity 1; blue yellow green laser 488/532/561nm, PE fluorescence intensity 2; red laser 633nm, APC fluorescence intensity 3 can conveniently contrast and refer to, and reagent box body 5's positive fixedly connected with box 7, and reagent box cover 1's left surface fixedly connected with son detains 8, and box 7 detains 8 looks joints with the son, can fix reagent box cover 1 and reagent box body 5.
The below of reagent box body 5 is equipped with two sets of symmetrical supporting pads 9, and the upper surface of every supporting pad 9 all with the bottom surface fixed connection of reagent box body 5, can conveniently support reagent box body 5, the last fixed surface of reagent lid 1 is connected with data plate 10, and the front of data plate 10 is equipped with label 11, can conveniently mark and record, conveniently seeks, and reagent box body 5 is the cuboid form, and reagent box body 5 is black shell, facilitates the use and deposits.
The utility model discloses a theory of operation is: in use, if the reagent box 5 is a staining protocol antibody box for human T cells, wherein the antibody dosage for detecting basic phenotype CD3/CD45/CD4/CD8 is large and is 5ml of bottled large branch antibody, and the antibody dosage for detecting cell typing CD62L/CD45RO/CCR7 is relatively small, and is 1ml of bottled small branch antibody, 7 antibodies with different sizes can exactly fill 7 hole sites, when in use, the whole lower right independent refrigerated flow type antibody rack 2 is put out, the antibodies can be preserved for a long time on the ice box, after use, the independent refrigerated flow type antibody rack 2 is returned to the reagent box 5, the reagent box 5 is stored in a refrigerator at 4 ℃ for a long time, and the other 5 independent refrigerated flow type antibody racks 2 can be a monocyte staining protocol, an NK cell staining protocol, a granulocyte staining protocol, a B cell staining protocol, in the storage and use areas of the matched antibodies such as specific tumor cell staining schemes and the like, when one whole peripheral blood needs to be subjected to flow analysis, one or more antibodies are extracted from the different staining schemes to be subjected to color matching combination, a new staining scheme is established, for example, T/B/NK/granulocytes are CD45 positive white blood cells, CD45 uses different colors in the respective staining schemes, the antibodies with the smallest cross color influence are required to be accurately matched according to the fluorescent spectrum 6 on the reagent box cover 1 and the channel of the flow cytometer, and the antibodies with the smallest cross color influence are selected to be used for the flow analysis of the whole blood.
The above description is only exemplary of the present invention and should not be construed as limiting the present invention, and any modifications, equivalents and improvements made within the spirit and principles of the present invention are intended to be included within the scope of the present invention.
Claims (6)
1. A flow antibody storage and use kit, including reagent lid (1), its characterized in that: the reagent box cover is characterized in that a reagent box body (5) is fixedly hinged to the bottom surface of the reagent box cover (1) through a crease, six independent refrigeration flow type antibody frames (2) are placed inside the reagent box body (5), a polyacrylic acid polyol polymer refrigerant is filled inside each independent refrigeration flow type antibody frame (2), small branch type antibody storage holes (3) which are arranged equidistantly are formed in the upper surfaces of the three independent refrigeration flow type antibody frames (2), large branch type antibody storage holes (4) which are arranged equidistantly are formed in the upper surface of one independent refrigeration flow type antibody frame (2), and the small branch type antibody storage holes (3) and the large branch type antibody storage holes (4) which are arranged equidistantly are formed in the upper surfaces of the two independent refrigeration flow type antibody frames (2).
2. A flow-through antibody storage and use kit as claimed in claim 1, wherein: the front surface of the reagent box cover (1) is provided with fluorescence intensity and a fluorescence spectrum (6) of common fluorescence names under each laser of the flow cytometer.
3. A flow-through antibody storage and use kit as claimed in claim 1, wherein: the positive fixedly connected with box (7) of reagent box body (5), the left surface fixedly connected with son of reagent lid (1) is detained (8), box (7) are detained (8) looks joint with the son.
4. A flow-through antibody storage and use kit as claimed in claim 1, wherein: two sets of symmetrical supporting pads (9) are arranged below the reagent box body (5), and each supporting pad (9) is fixedly connected with the bottom surface of the reagent box body (5) on the upper surface.
5. A flow-through antibody storage and use kit as claimed in claim 1, wherein: the reagent box cover is characterized in that a nameplate (10) is fixedly connected to the upper surface of the reagent box cover (1), and a label (11) is arranged on the front surface of the nameplate (10).
6. A flow-through antibody storage and use kit as claimed in claim 1, wherein: the reagent box body (5) is cuboid, and the reagent box body (5) is a black shell.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021957353.8U CN213769814U (en) | 2020-09-09 | 2020-09-09 | Flow type antibody storage and use kit |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021957353.8U CN213769814U (en) | 2020-09-09 | 2020-09-09 | Flow type antibody storage and use kit |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213769814U true CN213769814U (en) | 2021-07-23 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021957353.8U Active CN213769814U (en) | 2020-09-09 | 2020-09-09 | Flow type antibody storage and use kit |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213769814U (en) |
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2020
- 2020-09-09 CN CN202021957353.8U patent/CN213769814U/en active Active
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20220802 Address after: 201500 701e, building 5, No. 750, Linyuan street, Zhujing Town, Jinshan District, Shanghai Patentee after: Shanghai Aoneng Biotechnology Co.,Ltd. Address before: No. 58, Fumin Branch Road, Hengsha Township, Chongming District, Shanghai, 201914 (Shanghai Hengtai Economic Development Zone) Patentee before: Shanghai chener Biotechnology Center |
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| TR01 | Transfer of patent right |