CN213609920U - Alumina silica gel chromatographic column for eluting persistent organic matters - Google Patents
Alumina silica gel chromatographic column for eluting persistent organic matters Download PDFInfo
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- CN213609920U CN213609920U CN202022521178.4U CN202022521178U CN213609920U CN 213609920 U CN213609920 U CN 213609920U CN 202022521178 U CN202022521178 U CN 202022521178U CN 213609920 U CN213609920 U CN 213609920U
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Abstract
The utility model discloses an elution persistence organic matter alumina silica gel chromatographic column, include: the support, the upper portion of support is equipped with sleeve and cover barrel casing and connects on the surface of support and rather than sliding connection, the telescopic side is equipped with the side of bracing piece and is equipped with the mount, the inside of mount is equipped with fixed connection between tubular column and the mount, the upper portion of tubular column is equipped with into hopper and the lower part is equipped with the bushing. The utility model discloses set gradually the aluminium oxide layer in the inside from the bottom up of tubular column, neutral silica gel, alkaline silica gel and acid silica gel, neutral silica gel is provided with two sets ofly and is located between aluminium oxide layer and the alkaline silica gel and between acid silica gel and the alkaline silica gel respectively, wherein the tubular column diameter is 10 mm, length is 450 mm, aluminium oxide layer 6cm, neutral silica gel 2cm, alkaline silica gel 5 cm, neutral silica gel 2cm, acid silica gel 6cm, form multistage silica gel alumina column, because accurate ratio, the separation effect has been improved greatly.
Description
Technical Field
The utility model relates to a chromatographic column technical field, more specifically is an elution persistence organic matter alumina silica gel chromatographic column.
Background
Chromatography is short for "chromatography". The method is a method for separating and analyzing substances by utilizing the difference of the moving speed of the substances in a certain matrix based on the difference of physical and chemical properties of the substances. For example: the purpose of separating substances from each other is achieved by utilizing the differences of the substances in the aspects of solubility, adsorption capacity, stereochemical characteristics, molecular size, charging condition, ion exchange, affinity and the like. Generally, it is used for analysis of organic compounds, metal ions, amino acids, and the like.
The mechanism of chromatography can be roughly divided into: adsorption chromatography, partition chromatography, ion exchange chromatography, gel filtration chromatography, affinity chromatography, etc. The adsorption chromatography is to utilize the difference of the adsorption performance of the surface of the adsorbent to different components to achieve the aim of separation and identification; partition chromatography: the separation is achieved by utilizing the different distribution coefficients of different components between the mobile phase and the stationary phase. Ion exchange chromatography separates targets using different components for their affinity to the ion exchanger; gel chromatography is the use of certain gels to separate different targets for the blocking of components of different molecular sizes. In addition, there are other standard classifications, such as gas chromatography and liquid chromatography, depending on the mobile phase and the stationary phase. The operation forms can be classified into column chromatography, paper chromatography, thin layer chromatography, high performance liquid chromatography, etc. In the process of analyzing the environmental organic pollutants, column chromatography is commonly used: that is, the stationary phase is loaded in a column and the sample is moved in one direction to achieve separation.
In the prior art, the adsorption force of alumina and chromatographic silica gel used as adsorbents on different components of crude oil families is different, the speed and the required time of eluent flowing through the alumina and the chromatographic silica gel are different, and the elution force of the crude oil family components in the alumina and the chromatographic silica gel is different, so that the optimal separation effect cannot be achieved. Therefore, a new technical solution needs to be provided.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an elution persistence organic matter alumina silica gel chromatographic column, it is different to its adsorption affinity to the different components of crude oil clan to alumina and chromatography silica gel that use as the adsorbent among the prior art to have solved, and the speed when the eluant flows through alumina and chromatography silica gel is also different with required time, and the elution power of crude oil clan component also is different in elution alumina and chromatography silica gel, can't reach the problem of best separation effect.
In order to achieve the above object, the utility model provides a following technical scheme: an alumina silica gel chromatographic column for eluting persistent organics, comprising: the utility model provides a support, the upper portion of support is equipped with sleeve and cover barrel cup joint on the surface of support and rather than sliding connection, the side that telescopic side was equipped with bracing piece and bracing piece is equipped with the mount, the inside of mount is equipped with fixed connection between tubular column and the mount, the upper portion of tubular column is equipped with into hopper and lower part is equipped with the bushing, the opening diameter of going into the hopper is greater than the diameter of tubular column, the inside of tubular column is equipped with aluminium oxide layer, neutral silica gel layer, alkaline silica gel layer and acid silica gel layer by going up in proper order, the neutral silica gel layer is provided with two sets ofly and is located respectively between aluminium oxide layer and the alkaline silica gel layer and between acid silica gel layer and the alkaline silica gel layer, the bottom of tubular column is equipped with cotton wool-wool and cotton-wool layer and contacts each other, the inside of bushing is.
As a preferred embodiment of the present invention, the side of the sleeve is provided with a locking member and the locking member is connected with the sleeve by screw and contacts with the bracket.
As a preferred embodiment of the present invention, the side of the fixing frame is provided with a connecting block and the fixing frame is fixed on the surface of the tubular column through a bolt through connecting block.
As an optimal implementation manner of the present invention, the surface of the tubular column is provided with scale marks and the scale marks correspond to the alumina layer, the neutral silica gel layer, the alkaline silica gel layer and the acidic silica gel layer.
As a preferred embodiment of the utility model, the tubular column diameter is 10 mm and length is 450 mm, the aluminium oxide layer is 6cm, neutral silica gel is 2cm, alkaline silica gel is 5 cm, acid silica gel is 6 cm.
Compared with the prior art, the beneficial effects of the utility model are as follows:
the utility model discloses set up the sleeve and the side of sleeve sets up the bracing piece on the upper portion of support, the side of bracing piece sets up the mount and the inside of mount sets up the tubular column, slide between sleeve and the support, can height-adjusting, the top of tubular column sets up into the hopper, make things convenient for the interpolation of material, the bottom of tubular column sets up the weeping pipe and the surface of flow tube is equipped with the cock, can adjust its speed of outflow through rotatory cock, simultaneously in the inside from the bottom up of tubular column set gradually alumina layer, neutral silica gel, alkaline silica gel and acid silica gel, neutral silica gel is provided with two sets of and lies in respectively between alumina layer and alkaline silica gel and between acid silica gel and alkaline silica gel, wherein the tubular column diameter is 10 mm, the length is 450 mm, alumina layer 6cm, neutral silica gel 2cm, alkaline silica gel 5 cm, neutral silica gel 2cm, acid silica gel 6cm, due to the accurate proportioning, the separation effect is greatly improved.
Drawings
FIG. 1 is a schematic view of the overall structure of the present invention;
fig. 2 is the schematic diagram of the internal structure of the pipe column of the present invention.
In the figure, 1, a bracket; 2. a sleeve; 3. a locking member; 4. a support bar; 5. a fixed mount; 6. connecting blocks; 7. feeding into a hopper; 8. a pipe string; 9. scale lines; 10. a cock; 11. a leak pipe; 12. an acidic silica gel layer; 13. a neutral silica gel layer; 14. an alkaline silica gel layer; 15. an aluminum oxide layer; 16. absorbent cotton.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-2, the present invention provides a technical solution: an alumina silica gel chromatographic column for eluting persistent organics, comprising: the support comprises a support 1, the upper portion of the support 1 is provided with a sleeve 2, the sleeve 2 is sleeved on the surface of the support 1 and is in sliding connection with the support, a fixing frame 5 is arranged on the side face of the sleeve 2, supporting rods 4 are arranged on the side face of the supporting rods 4, a tubular column 8 is arranged inside the fixing frame 5, the tubular column 8 is fixedly connected between the tubular column 8 and the fixing frame 5, the supporting rod 4 is arranged on the side face of the sleeve 2 and the side face of the sleeve 2 on the upper portion of the support 1, the tubular column 8 is arranged inside the fixing frame 5 on the side face of the supporting rods 4, the sleeve 2 and the support 1 slide to adjust the height, a hopper 7 is arranged at the top of the tubular column 8, the hopper 7 is convenient to add materials, the hopper 7 is arranged on the upper portion of the tubular column 8, a leakage pipe 11 is arranged on the lower portion of the tubular column, Alkaline silica gel layer 14 and acid silica gel layer 12, neutral silica gel layer 13 is provided with two sets and is located respectively between aluminium oxide layer 15 and alkaline silica gel layer 14 and between acid silica gel layer 12 and alkaline silica gel layer 14, the bottom of tubular column 8 is equipped with absorbent cotton 16 and aluminium oxide layer 15 mutual contact, aluminium oxide layer 15, neutral silica gel, alkaline silica gel and acid silica gel set gradually from the bottom up in the inside of tubular column 8, neutral silica gel is provided with two sets and is located respectively between aluminium oxide layer 15 and alkaline silica gel and between acid silica gel and the alkaline silica gel, wherein tubular column 8 diameter is 10 mm, length is 450 mm, aluminium oxide layer 156cm, neutral silica gel 2cm, alkaline silica gel 5 cm, neutral silica gel 2cm, acid silica gel 6cm form multistage silica gel aluminium oxide column, because accurate ratio, separation effect has greatly improved, the inside of leak pipe 11 is equipped with cock 10 and the rotation is connected between cock 10 and leak pipe 11, the speed of its outflow can be adjusted by rotating the tap 10.
Further improved, as shown in fig. 1: the side of sleeve 2 is equipped with retaining member 3 and 2 between threaded connection and with support 1 between mutual contact, the stability of sleeve 2 has been guaranteed in the setting of retaining member 3.
Further improved, as shown in fig. 1: the side of mount 5 is equipped with connecting block 6 and mount 5 and fixes on 8 surfaces of tubular column through connecting block 6 through the bolt, and the connecting pipe is locked through the bolt, has guaranteed the fixed stability of mount 5.
Further improved, as shown in fig. 1: the surface of the tubular column 8 is provided with scale marks 9, the scale marks 9 correspond to the alumina layer 15, the neutral silica gel layer 13, the alkaline silica gel layer 14 and the acidic silica gel layer 12, and the heights of the alumina layer 15, the neutral silica gel layer 13, the alkaline silica gel layer 14 and the acidic silica gel layer 12 in the tubular column 8 can be visually known through the arrangement of the scale marks 9.
Further improved, as shown in fig. 2: the diameter of the tubular column 8 is 10 mm and the length is 450 mm, the alumina layer 15 is 6cm, the neutral silica gel is 2cm, the alkaline silica gel is 5 cm, and the acidic silica gel is 6 cm.
The utility model discloses the side that sets up sleeve 2 and sleeve 2 on the upper portion of support 1 sets up bracing piece 4, the inside that the side of bracing piece 4 set up mount 5 and mount 5 sets up tubular column 8, slide between sleeve 2 and the support 1, can height-adjusting, the top of tubular column 8 sets up into hopper 7, make things convenient for the interpolation of material, the bottom of tubular column 8 sets up that leak pipe 11 and the surface of flow tube are equipped with cock 10, can adjust its speed of flowing through rotatory cock 10, simultaneously set gradually aluminium oxide layer 15 from the bottom up in the inside of tubular column 8, neutral silica gel, alkaline silica gel and acid silica gel, neutral silica gel is provided with two sets ofly and is located respectively between aluminium oxide layer 15 and the alkaline silica gel and between acid silica gel and the alkaline silica gel, wherein tubular column 8 diameter is 10 mm, length is 450 mm, aluminium oxide layer 156cm, neutral silica gel 2cm, alkaline silica gel 5, Neutral silica gel 2cm and acid silica gel 6cm to form several sections of silica gel-alumina column, and this raises the separation effect greatly.
Finally, it should be noted that: although the present invention has been described in detail with reference to the foregoing embodiments, it will be apparent to those skilled in the art that modifications may be made to the embodiments described in the foregoing embodiments, or equivalents may be substituted for elements thereof. Any modification, equivalent replacement, or improvement made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (5)
1. An alumina silica gel chromatographic column for eluting persistent organic matters is characterized in that: the method comprises the following steps: the device comprises a support (1), wherein a sleeve (2) is arranged on the upper portion of the support (1), the sleeve (2) is sleeved on the surface of the support (1) and is in sliding connection with the support, a support rod (4) is arranged on the side face of the sleeve (2), a fixing frame (5) is arranged on the side face of the support rod (4), a tubular column (8) is arranged inside the fixing frame (5), the tubular column (8) is fixedly connected with the fixing frame (5), a feeding hopper (7) is arranged on the upper portion of the tubular column (8), a leakage pipe (11) is arranged on the lower portion of the tubular column (8), the opening diameter of the feeding hopper (7) is larger than that of the tubular column (8), an alumina layer (15), a neutral silica gel layer (13), an alkaline silica gel layer (14) and an acidic silica gel layer (12) are sequentially arranged inside the tubular column (8) from bottom to top, the neutral silica gel layer (13) is provided with two groups and respectively located between the alumina layer (15) and the alkaline silica gel, the bottom of the pipe column (8) is provided with absorbent cotton (16), the absorbent cotton (16) is in contact with the alumina layer (15), the inside of the leakage pipe (11) is provided with a cock (10), and the cock (10) is rotationally connected with the leakage pipe (11).
2. The elution permanent organic alumina silica gel column of claim 1, wherein: the side of sleeve (2) is equipped with retaining member (3) and threaded connection between retaining member (3) and sleeve (2) and with support (1) between mutual contact.
3. The elution permanent organic alumina silica gel column of claim 1, wherein: the side of the fixing frame (5) is provided with a connecting block (6) and the fixing frame (5) is fixed on the surface of the pipe column (8) through the connecting block (6) by bolts.
4. The elution permanent organic alumina silica gel column of claim 1, wherein: the surface of the tubular column (8) is provided with scale marks (9), and the scale marks (9) correspond to the alumina layer (15), the neutral silica gel layer (13), the alkaline silica gel layer (14) and the acidic silica gel layer (12).
5. The elution permanent organic alumina silica gel column of claim 4, wherein: the diameter of the tubular column (8) is 10 mm and the length is 450 mm, the alumina layer (15) is 6cm, the neutral silica gel is 2cm, the alkaline silica gel is 5 cm, and the acidic silica gel is 6 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022521178.4U CN213609920U (en) | 2020-11-04 | 2020-11-04 | Alumina silica gel chromatographic column for eluting persistent organic matters |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202022521178.4U CN213609920U (en) | 2020-11-04 | 2020-11-04 | Alumina silica gel chromatographic column for eluting persistent organic matters |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113577823A (en) * | 2021-07-26 | 2021-11-02 | 云南聚云基因工程有限公司 | Glucan gel chromatographic column and method for separating umbilical cord blood mesenchymal stem cell exosome |
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- 2020-11-04 CN CN202022521178.4U patent/CN213609920U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113577823A (en) * | 2021-07-26 | 2021-11-02 | 云南聚云基因工程有限公司 | Glucan gel chromatographic column and method for separating umbilical cord blood mesenchymal stem cell exosome |
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