CN213476002U - Improved cell culture plate - Google Patents
Improved cell culture plate Download PDFInfo
- Publication number
- CN213476002U CN213476002U CN202021140084.6U CN202021140084U CN213476002U CN 213476002 U CN213476002 U CN 213476002U CN 202021140084 U CN202021140084 U CN 202021140084U CN 213476002 U CN213476002 U CN 213476002U
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- Prior art keywords
- plate body
- cup
- plate
- cell culture
- cell
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- 238000004113 cell culture Methods 0.000 title claims abstract description 33
- 229920001343 polytetrafluoroethylene Polymers 0.000 claims description 5
- 239000004810 polytetrafluoroethylene Substances 0.000 claims description 5
- -1 polytetrafluoroethylene Polymers 0.000 claims description 4
- 238000002474 experimental method Methods 0.000 abstract description 16
- 230000009194 climbing Effects 0.000 abstract description 8
- RTAQQCXQSZGOHL-UHFFFAOYSA-N Titanium Chemical compound [Ti] RTAQQCXQSZGOHL-UHFFFAOYSA-N 0.000 abstract description 7
- 229910052719 titanium Inorganic materials 0.000 abstract description 7
- 239000010936 titanium Substances 0.000 abstract description 7
- 230000010261 cell growth Effects 0.000 abstract description 6
- 230000006872 improvement Effects 0.000 abstract description 5
- 238000012545 processing Methods 0.000 abstract description 3
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000004140 cleaning Methods 0.000 description 5
- 230000006907 apoptotic process Effects 0.000 description 3
- 238000013461 design Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 238000009825 accumulation Methods 0.000 description 2
- 230000001464 adherent effect Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000002699 waste material Substances 0.000 description 2
- 241001391944 Commicarpus scandens Species 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000005260 corrosion Methods 0.000 description 1
- 230000007797 corrosion Effects 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 231100000956 nontoxicity Toxicity 0.000 description 1
- 230000000149 penetrating effect Effects 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000002000 scavenging effect Effects 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 239000002356 single layer Substances 0.000 description 1
- 230000000638 stimulation Effects 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000005406 washing Methods 0.000 description 1
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Abstract
The utility model provides a cell culture plate of improvement, which comprises a plate body, set up a plurality of cultivation holes that link up the plate body on the plate body, cultivate the downthehole cultivation cup that is equipped with, the top protrusion of cultivating the cup in the upper surface of plate body, the bottom evenly distributed who cultivates the cup has a plurality of support columns, install the movable cover that can overturn with the cultivation cup one-to-one on the plate body. The utility model has the advantages of simple and reasonable structure, be convenient for production, the processing cost is low, can the auxiliary operation make things convenient for, swiftly press from both sides the thin slice carrier devices such as cell climbing piece or the titanium piece that are used for the cell culture carrier in getting the culture cup, avoid in the operation cell climbing piece appear broken or thin slice carrier device be difficult to press from both sides the circumstances of getting, can carry out solitary experiment in different holes at different time point pair simultaneously and handle, not only prevent the circumstances of cell pollution and extravagant resource, still ensured the repeatability of cell growth environment in the experiment.
Description
Technical Field
The utility model belongs to the technical field of cell culture, especially, relate to a cell culture plate of improvement.
Background
In the cell experiment operation, a sheet device such as a titanium sheet or a cell slide is often used as a cell carrier, an operator usually needs to place the sheet carrier in a culture hole, and the sheet carrier is taken out after the cell growth slide is finished, however, because the bottom of the cell culture plate is generally a smooth plane, the sheet carrier device such as the titanium sheet or the cell slide can be tightly attached to the bottom of the culture plate due to factors such as siphon and the like, and is difficult to take out, time and labor are wasted, sometimes, the cell growth state is not good, and the severe condition of cell apoptosis can occur; wherein the cell climbing sheet made of glass material is generally very thin, the operation difficulty is higher, and the cell climbing sheet made of glass material is easy to break by slightly applying force. Meanwhile, because the traditional culture plate is a whole, when a certain culture cup is subjected to cell treatment, all the culture cups in the culture plate can only be treated at the same time in order to prevent the adjacent holes from causing cell pollution, but sometimes experiment design needs to treat the same cells at different times, or the same stimulation time is inconsistent, so that only a plurality of culture plates can be used for experiment, great resource waste is caused, the condition that the cell growth environment is inconsistent can also occur, and certain influence can be caused on the experiment result.
SUMMERY OF THE UTILITY MODEL
In view of this, the utility model aims at providing an improved cell culture plate, its aim at solve present use titanium sheet or cell climbing piece etc. thin slice carrier device in current culture plate when, take out inconveniently and lead to the easy breakage of glass cell climbing piece even, simultaneously, can also be on same culture plate, freely carry out experimental treatment to single culture cup and do not influence the use of other culture cups.
In order to achieve the above purpose, the technical scheme of the utility model is realized like this:
the utility model provides a cell culture board of improvement, the package rubbing board body, the bottom of plate body is equipped with the landing leg respectively all around, set up a plurality of cultivation holes that link up the plate body on the plate body, cultivate the downthehole culture cup that is equipped with, the top protrusion in the upper surface of plate body of culture cup, the bottom evenly distributed of culture cup has a plurality of support columns, install the movable cover that can overturn with culture cup one-to-one on the plate body.
Furthermore, a support is arranged on one side of the movable cover on the plate body, a rotating shaft is rotatably arranged at the top of the support, one end of the movable cover is fixed on the rotating shaft, and a protruding bump is arranged at the other end of the movable cover.
Furthermore, the shape of the support column is a cylinder or a cube.
Furthermore, a circle of bulge is arranged on the lower surface of the movable cover, and the inner diameter of the bulge is slightly larger than the outer diameter of the culture cup.
Furthermore, a circle of flange is fixed on the outer wall of the upper part of the culture cup, the plate body is inwards sunken on the outer side of the culture cup to form a step matched with the flange, and the flange is placed on the step.
Furthermore, inserting columns which correspond to the supporting legs one to one are arranged on the periphery of the upper surface of the plate body, inserting holes are formed in the tops of the inserting columns, and the size of each inserting hole is slightly larger than that of each supporting leg.
Furthermore, the plate body, the supporting legs and the movable cover are all made of polytetrafluoroethylene.
Compared with the prior art, the improved cell culture plate of the utility model has the following advantages:
(1) the utility model discloses the improvement has designed the cell culture board, in cell biology experiment, when needing to use thin slice carrier devices such as titanium sheet or cell slide, after the cell culture, help the operator to take out thin slice carrier device from cultivating the cup bottom plate fast simply, can avoid the operator to destroy because of the misoperation and have already adhered to the wall the growing cell on the one hand, or take out the thin slice carrier device operating time longer and lead to the cell apoptosis that the large tracts of land has already adhered to the wall the growing; on the other hand, the probability of cell slide breakage caused by improper operation can be greatly reduced, so that the success rate of an experiment operator is improved, a large amount of precious time and energy are saved for the operator, and good experiment quality is ensured;
(2) cultivate and be fixed with the round flange on the upper portion outer wall of cup, on the plate body in the outside of cultivateing the cup inwards sunken form with flange assorted step, the flange place in on the step for cultivate the cup and can be taken off alone for with cell direct contact cultivate the cup dismantled and assembled, after certain or some cultivation cup on this cell culture board is used, can dismantle cleaning and disinfecting alone, greatly reduced scavenging period and cleaning cost. In the cleaning process, the rest unused culture cups can be used;
(3) the upper surface of plate body is equipped with the spliced pole with the landing leg one-to-one all around, the spliced eye has been seted up at the top of spliced pole, the size of spliced eye slightly is greater than the size of landing leg, through the grafting design for the culture plate can range upon range of the accumulation, saves occupation space, has effectively made things convenient for staff's work, has satisfied the use needs.
Drawings
The accompanying drawings, which form a part hereof, are included to provide a further understanding of the invention, and are incorporated in and constitute a part of this specification, illustrate embodiments of the invention and together with the description serve to explain the invention without undue limitation. In the drawings:
FIG. 1 is a perspective view of an improved cell culture plate according to an embodiment of the present invention;
FIG. 2 is a side view of an improved cell culture plate according to an embodiment of the present invention;
FIG. 3 is a cross-sectional view taken along line A-A of FIG. 2;
FIG. 4 is a top view of an improved cell culture plate according to an embodiment of the present invention;
FIG. 5 is a cross-sectional view taken along line B-B of FIG. 4;
FIG. 6 is a top view of a sheet carrier device placed in a culture cup of an improved cell culture plate according to an embodiment of the present invention;
FIG. 7 is a cross-sectional view of a modified cell culture plate according to an embodiment of the present invention with a thin sheet carrier device placed in the culture cup;
FIG. 8 is a schematic view of a cell culture plate according to an embodiment of the present invention in a stacked state.
Description of reference numerals:
1. a plate body; 2. a support leg; 3. a culture cup; 4. a support pillar; 5. a movable cover; 6. a support; 7. a rotating shaft; 8. a bump; 9. a protrusion; 10. a flange; 11. a step; 12. inserting the column; 13. and (7) inserting holes.
Detailed Description
It should be noted that, in the present invention, the embodiments and features of the embodiments may be combined with each other without conflict.
In the description of the present invention, it is to be understood that the terms "center", "longitudinal", "lateral", "up", "down", "front", "back", "left", "right", "vertical", "horizontal", "top", "bottom", "inner", "outer", and the like, indicate orientations or positional relationships based on the orientations or positional relationships shown in the drawings, and are used merely for convenience of description and for simplicity of description, and do not indicate or imply that the device or element being referred to must have a particular orientation, be constructed and operated in a particular orientation, and therefore, should not be construed as limiting the present invention. Furthermore, the terms "first", "second", etc. are used for descriptive purposes only and are not to be construed as indicating or implying relative importance or implicitly indicating the number of technical features indicated.
In the description of the present invention, it is to be noted that, unless otherwise explicitly specified or limited, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be, for example, fixedly connected, detachably connected, or integrally connected; can be mechanically or electrically connected; they may be connected directly or indirectly through intervening media, or they may be interconnected between two elements. The specific meaning of the above terms in the present invention can be understood by those of ordinary skill in the art through specific situations.
The present invention will be described in detail below with reference to the accompanying drawings in conjunction with embodiments.
An improved cell culture plate is mainly used for cell culture of sheet carrier devices such as cell crawlers and the like, and plays a role in flexibly processing single-hole cells. As shown in fig. 1-8, the plate comprises a plate body 1, wherein the periphery of the bottom of the plate body 1 is provided with supporting legs 2, a proper number of culture holes penetrating through the plate body 1 are formed in the plate body 1 as required, culture cups 3 with the same size and shape are arranged in the culture holes, and the tops of the culture cups 3 protrude out of the upper surface of the plate body 1.
In a cell biology experiment, when a titanium sheet or a cell slide or other sheet carrier device is needed to be used, after cell culture is finished, an operator is helped to conveniently, quickly and simply take out the sheet carrier device from a bottom plate of a culture cup 3, so that on one hand, the phenomenon that the operator destroys cells which grow adherent to the wall due to improper operation or the apoptosis of the cells which grow adherent to a large area due to long operation time for taking out the sheet carrier device can be avoided; on the other hand, the probability of cell slide breakage caused by improper operation can be greatly reduced. Therefore, the success rate of the experiment operator is improved, a great amount of precious time and energy are saved for the operator, and meanwhile, good experiment quality is guaranteed.
The bottom of the culture cup 3 is uniformly distributed with 10 raised support columns 4, and the support columns 4 are cylindrical or cubic. The utility model discloses a culture cup, including plate body 1, plate body 5, support 6, pivot 7, the one end of movable cover 5 is fixed in on the pivot 7, the other end of movable cover 5 is equipped with outstanding lug 8 to the convenient flip movable cover 5 that can overturn that corresponds with culture cup 3 one-to-one, it is concrete, be equipped with on the plate body 1 in one side of movable cover 5 and transversely place, the opening is up "]" shape support 6, the top of support 6 is rotated and is equipped with pivot 7, the one end of movable cover 5 is fixed in on the pivot 7, the other end. The movable cover is arranged above each culture cup 3, so that independent experiment treatment can be carried out on different holes at different time points, the conditions of cell pollution and resource waste are prevented, and the repeatability of the cell growth environment in the experiment is ensured.
The lower surface of the movable cover 5 is provided with a circle of bulges 9 to enhance the sealing performance of the movable cover 5, and the inner diameter of the bulges 9 is slightly larger than the outer diameter of the culture cup 3, so that the movable cover 5 can be conveniently covered.
A circle of flange 10 is fixed on the outer wall of the upper part of the culture cup 3, the outer side of the culture cup 3 on the plate body 1 is inwards recessed to form a step 11 matched with the flange 10, the flange 10 is placed on the step 11, so that the culture cup 3 can be independently taken down and can be detached and used for the culture cup 3 which is in direct contact with cells, when a certain culture cup or certain culture cups 3 on the cell culture plate are used, the culture cup or the culture cups can be independently detached, cleaned and disinfected, and the cleaning time and the cleaning cost are greatly reduced. And the remaining unused culture cups 3 can be used during the washing process.
Usually, the cell culture plate can only be placed in a single layer, and occupies a relatively large space, so that the culture plate can occupy more space when being placed in an incubator, and cell culture is affected. In order to solve the problems, inserting columns 12 which correspond to the supporting legs 2 one to one are arranged on the periphery of the upper surface of the plate body 1, inserting holes 13 are formed in the tops of the inserting columns 12, and the size of each inserting hole 13 is slightly larger than that of each supporting leg 2. Through the design of pegging graft for the cultivation board can range upon range of the accumulation, saves occupation space, has effectively made things convenient for staff's work, has satisfied the use needs.
The plate body 1, the supporting legs 2 and the movable cover 5 are all made of polytetrafluoroethylene, and Polytetrafluoroethylene (PTFE) is a high-molecular compound, so that the plate body has good chemical stability, corrosion resistance and ageing resistance, and can be used for a long time. Has physiological inertia and no toxicity, and is beneficial to the culture of cells; has high temperature resistance, and can be sterilized at high temperature.
The utility model has the advantages of simple and reasonable structure, be convenient for production, the processing cost is low, can the auxiliary operation make things convenient for, swiftly press from both sides the thin slice carrier devices such as cell climbing piece or the titanium piece that are used for the cell culture carrier in getting the culture cup, avoid in the operation cell climbing piece appear broken or thin slice carrier device be difficult to press from both sides the circumstances of getting, can carry out solitary experiment in different holes at different time point pair simultaneously and handle, not only prevent the circumstances of cell pollution and extravagant resource, still ensured the repeatability of cell growth environment in the experiment.
The above description is only a preferred embodiment of the present invention, and should not be taken as limiting the invention, and any modifications, equivalent replacements, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (7)
1. An improved cell culture plate, comprising: the plate comprises a plate body (1), the bottom of plate body (1) is equipped with landing leg (2) all around respectively, set up a plurality of cultivation holes that link up plate body (1) on plate body (1), cultivate downthehole being equipped with and cultivate cup (3), the top protrusion in the upper surface of plate body (1) of cultivating cup (3), the bottom evenly distributed who cultivates cup (3) has a plurality of support columns (4), install movable cover (5) that can overturn with cultivation cup (3) one-to-one on plate body (1).
2. The improved cell culture plate of claim 1, wherein: the novel plate is characterized in that a support (6) is arranged on one side of the movable cover (5) on the plate body (1), a rotating shaft (7) is arranged at the top of the support (6) in a rotating mode, one end of the movable cover (5) is fixed on the rotating shaft (7), and a protruding bump (8) is arranged at the other end of the movable cover (5).
3. The improved cell culture plate of claim 1, wherein: the supporting column (4) is cylindrical or cubic.
4. The improved cell culture plate of claim 1, wherein: the lower surface of the movable cover (5) is provided with a circle of bulges (9), and the inner diameter of each bulge (9) is slightly larger than the outer diameter of the culture cup (3).
5. The improved cell culture plate of claim 1, wherein: a circle of flange (10) is fixed on the outer wall of the upper part of the culture cup (3), the outer side of the culture cup (3) on the plate body (1) is inwards sunken to form a step (11) matched with the flange (10), and the flange (10) is placed on the step (11).
6. The improved cell culture plate of claim 1, wherein: the plate is characterized in that inserting columns (12) which correspond to the supporting legs (2) one to one are arranged on the periphery of the upper surface of the plate body (1), inserting holes (13) are formed in the tops of the inserting columns (12), and the sizes of the inserting holes (13) are slightly larger than those of the supporting legs (2).
7. The improved cell culture plate of claim 1, wherein: the plate body (1), the supporting legs (2) and the movable cover (5) are all made of polytetrafluoroethylene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021140084.6U CN213476002U (en) | 2020-06-18 | 2020-06-18 | Improved cell culture plate |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021140084.6U CN213476002U (en) | 2020-06-18 | 2020-06-18 | Improved cell culture plate |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213476002U true CN213476002U (en) | 2021-06-18 |
Family
ID=76390646
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021140084.6U Expired - Fee Related CN213476002U (en) | 2020-06-18 | 2020-06-18 | Improved cell culture plate |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213476002U (en) |
-
2020
- 2020-06-18 CN CN202021140084.6U patent/CN213476002U/en not_active Expired - Fee Related
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210618 |