CN213466854U - Simple antibody purification chromatographic column - Google Patents
Simple antibody purification chromatographic column Download PDFInfo
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- CN213466854U CN213466854U CN202022120698.4U CN202022120698U CN213466854U CN 213466854 U CN213466854 U CN 213466854U CN 202022120698 U CN202022120698 U CN 202022120698U CN 213466854 U CN213466854 U CN 213466854U
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- sets
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- downside
- antibody purification
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- 238000011091 antibody purification Methods 0.000 title claims abstract description 16
- 238000004587 chromatography analysis Methods 0.000 claims abstract 7
- 238000004140 cleaning Methods 0.000 abstract description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 abstract 2
- 229910052742 iron Inorganic materials 0.000 abstract 1
- 239000012488 sample solution Substances 0.000 description 8
- 238000000034 method Methods 0.000 description 7
- 238000000746 purification Methods 0.000 description 7
- 230000000694 effects Effects 0.000 description 4
- 238000003780 insertion Methods 0.000 description 3
- 230000037431 insertion Effects 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 238000007790 scraping Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 241000283973 Oryctolagus cuniculus Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 230000005489 elastic deformation Effects 0.000 description 1
- 238000001125 extrusion Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000010030 laminating Methods 0.000 description 1
- 210000003097 mucus Anatomy 0.000 description 1
- 210000002445 nipple Anatomy 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 239000000523 sample Substances 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
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- Treatment Of Liquids With Adsorbents In General (AREA)
Abstract
The utility model relates to a chromatography column technical field specifically is plain type antibody purification chromatography column, the electric iron comprises a cylinder body, the downside of cylinder is provided with the control valve, and the downside fixedly connected with needle tubing of control valve, the top of cylinder is provided with sealed lid, and the vertical connecting rod that is connected with of downside of sealed lid, the fixed cover in bottom of connecting rod has connect the cover block, and the outside of cover block is provided with two sets of scraper blades, the upper and lower both ends that scraper blade and cover block are close to one side each other have seted up the spout, and the inside all blocks of spout have the slider, one side that the slider is close to each other articulates there are two sets of hinge bars, and is two sets of the hinge bar is articulated each other, and is. The utility model discloses be provided with nest block and scraper blade, improve the cleaning efficiency to the cylinder, the reuse of being convenient for is provided with second spring and guard plate simultaneously, and then can effectively prolong the life of this device.
Description
Technical Field
The utility model relates to a chromatographic column technical field specifically is plain type antibody purification chromatographic column.
Background
Carry out purification separation to the antibody, especially from the conventional technique that the antibody preparation is to the separation purpose antibody in immune rabbit serum, therefore need use the chromatographic column to carry out auxiliary work, but current chromatographic column is after purification work, the cylinder inner wall easily adheres to have the solution to remain, because the cylinder internal diameter is less, it is more inconvenient to the cleaning process of cylinder inner wall, current chromatographic column needle tubing is comparatively slender in addition, easily receive the appearance that external force strikes and lead to the phenomenon of rupture, make chromatographic column life shorter, for this we have proposed plain type antibody purification chromatographic column and have solved above-mentioned problem.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide a plain type antibody purification chromatographic column to solve the current chromatographic column clearance process that proposes among the above-mentioned background art comparatively loaded down with trivial details and the needle tubing easily receives the problem that external force strikes the appearance that leads to the phenomenon of rupture.
In order to achieve the above object, the utility model provides a following technical scheme: plain type antibody purification chromatographic column, the novel nipple rectifier comprises a cylinder body, the downside of cylinder is provided with the control valve, and the downside fixedly connected with needle tubing of control valve, the top of cylinder is provided with sealed lid, and the vertical connecting rod that is connected with of downside of sealed lid, the fixed cover in bottom of connecting rod has connect the cover block, and the outside of cover block is provided with two sets of scrapers, the spout has been seted up at the upper and lower both ends that scraper blade and cover block are close to one side each other, and the inside all blocks of spout have the slider, one side that the slider is close to each other articulates there are two sets of hinge bar, and two sets of the hinge bar is articulated each other, two.
Preferably, the inner wall fixedly connected with spacing ring of cylinder upside, and the upside of spacing ring has placed two sets of rings of placing, and two sets of the downside of placing the ring all is provided with the filter screen, and is two sets of place one side difference fixedly connected with magnet that the ring is close to each other.
Preferably, the sliding sleeve is movably sleeved on the outer side of the column body, and the outer surface of the sliding sleeve is uniformly provided with the protruding blocks.
Preferably, the left end and the right end of the lower side of the control valve are respectively connected with a vertical rod through bolts, the inside of the vertical rod is transversely inserted with an insertion rod, one side of the insertion rod, which is close to each other, is fixedly connected with a protection plate, one side of the protection plate, which is far away from each other, is connected with a second spring, and the outer surface of the insertion rod is sleeved with the second spring.
Preferably, the cross section of the two groups of the scrapers is set to be an arc-shaped structure, and the upper sides of the two groups of the scrapers are set to be an inclined structure.
Preferably, the longitudinal sections of the two groups of filter screens are arranged to be arc-shaped structures, and the convex surfaces of the two groups of filter screens are downward.
Compared with the prior art, the beneficial effects of the utility model are that: the utility model discloses a through being provided with nest block and scraper blade, after purification work, accessible pulling sealed lid for two sets of scraper blades that sealed downside set up contact with the cylinder inner wall, clear up the mucus that remains in the purification process, easy operation is swift, improves the clearance efficiency to the cylinder, is convenient for reuse simultaneously;
through being provided with second spring and guard plate, the elastic characteristic who utilizes the second spring makes guard plate and needle tubing inner wall closely laminate, realizes the protection to the needle tubing outside, avoids the needle tubing to receive external force fracture in the use, strengthens the bulk strength of needle tubing, and then can effectively prolong the life of this device, is convenient for take out the guard plate from the device bottom simultaneously, is convenient for realize its reuse's effect.
Description of the drawings:
in order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments of the present invention, and for those skilled in the art, other drawings can be obtained according to these drawings without creative efforts.
FIG. 1 is a schematic sectional view of the front view of the structure of the present invention;
FIG. 2 is a schematic view of the working state of the present invention;
FIG. 3 is a schematic top sectional view of the structure of the present invention;
FIG. 4 is a schematic top view of the placement ring and screen structure of the present invention;
fig. 5 is a partially enlarged schematic view of a in fig. 2 according to the present invention;
fig. 6 is a partially enlarged schematic view of B in fig. 1 according to the present invention;
fig. 7 is a partially enlarged schematic view of C in fig. 1 according to the present invention.
In the figure: 1. a cylinder; 2. a control valve; 3. a needle tube; 4. a sealing cover; 5. a connecting rod; 6. sleeving blocks; 7. a squeegee; 8. a slider; 9. a hinged lever; 10. a first spring; 11. a limiting ring; 12. placing a ring; 13. filtering with a screen; 14. a magnet; 15. a vertical rod; 16. inserting the rod; 17. a protection plate; 18. a second spring; 19. a sliding sleeve.
The specific implementation mode is as follows:
the technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-7, the present invention provides an embodiment: the simple antibody purification chromatographic column comprises a column body 1, wherein a control valve 2 is arranged on the lower side of the column body 1, a needle tube 3 is fixedly connected to the lower side of the control valve 2, a sealing cover 4 is arranged at the top end of the column body 1, a connecting rod 5 is vertically connected to the lower side of the sealing cover 4, a sleeve block 6 is fixedly sleeved at the bottom end of the connecting rod 5, two groups of scraping plates 7 are arranged on the outer side of the sleeve block 6, sliding grooves are formed in the upper end and the lower end, close to one side, of each other, of each scraping plate 7 and the corresponding sleeve block 6, sliding blocks 8 are clamped inside the sliding grooves, two groups of hinge rods 9 are hinged to one side, close to each other, of the sliding;
further, according to fig. 1, as shown in fig. 4 and fig. 6, the inner wall fixedly connected with spacing ring 11 of 1 upside of cylinder, and the upside of spacing ring 11 has been placed two sets of rings 12 of placing, and two sets of downside of placing rings 12 all are provided with filter screen 13, two sets of one side of placing rings 12 each other and being close to respectively fixedly connected with magnet 14, during the use, can place two sets of rings 12 and be close to each other and connect through magnet 14, can place two sets of rings 12 in the upside of spacing ring 11 after the connection is accomplished, can upwards stimulate simultaneously and place rings 12, make and place ring 12 downside and spacing ring 11 contactless again, be convenient for take out filter screen 13 from 1 inner wall of cylinder, in time change and clearance, improve filter screen 13's result of use.
Further, as shown in the accompanying drawings 1 and 2, the sliding sleeve 19 is movably sleeved on the outer side of the cylinder 1, the outer surface of the sliding sleeve 19 is uniformly provided with the convex blocks, the sliding sleeve 19 is made of transparent rubber, the friction coefficient between the outer wall of the cylinder 1 and the hand of a user is improved when the observation of the user is not influenced, scratches are prevented from appearing on the outer wall of the cylinder 1 when the device is used, and the service life of the device is prolonged.
Further, as shown in fig. 1, fig. 2 and fig. 5, the left and right ends of the lower side of the control valve 2 are respectively connected with a vertical rod 15 through bolts, an inserting rod 16 is transversely inserted into the vertical rod 15, a protection plate 17 is fixedly connected to one side of the inserting rod 16 close to each other, a second spring 18 is connected to one side of the protection plate 17 far away from each other, the second spring 18 is sleeved on the outer surface of the inserting rod 16, two groups of vertical rods 15 are mounted on the lower side of the control valve 2 through bolts, so that the outer surface of the needle tube 3 is tightly attached to the protection plate 17, the inserting rod 16 is driven to move inside the vertical rod 15 in the stress process of the protection plate 17, the second spring 18 sleeved on the outer side of the inserting rod 16 is stressed to deform through the elastic characteristics of the second spring 18, the protection plate 17 is tightly attached to the outer wall of the needle tube 3 through the elasticity, after the use, can take out it from the needle tubing 3 outside, be convenient for the later stage and use.
Further, as shown in the accompanying drawings 1, 2 and 5, the cross section of the two groups of scraper blades 7 is set to be an arc-shaped structure, the upper sides of the two groups of scraper blades 7 are set to be an inclined structure, the arc-shaped structure is arranged, so that the scraper blades 7 are repeatedly contacted with the inner wall of the cylinder 1, and the upper sides of the scraper blades 7 are inclined, so that the scraper blades 7 are close to the limiting ring 11 when in contact with the limiting ring under the action of stress, and the scraper blades 7 can be conveniently taken out from the inside of the cylinder 1, and the purification effect.
Further, as shown in the accompanying drawings 1, 4 and 6, the longitudinal section of two sets of filter screens 13 is set to be an arc structure, the convex surfaces of the two sets of filter screens 13 are downward, and in the process of sample introduction, the sample solution can be poured into the cylinder 1, so that the sample solution falls on the filter screens 13, and the sample solution flows down from the center position inside the cylinder 1 through the diversion of the filter screens 13, thereby avoiding the sample solution from splashing due to contact with the inner wall of the cylinder 1 in the pouring process, and ensuring the antibody purification effect.
The working principle is as follows: during the use, usable bolt is installed two sets of montants 15 at the downside of control valve 2 for the surface and the guard plate 17 of needle tubing 3 closely laminate, and the in-process of guard plate 17 atress drives and inserts and establish pole 16 at montant 15 inside removal, inserts the second spring 18 atress that establishes the pole 16 outside and cup joint this moment and takes place deformation through self elastic characteristic, utilizes the elasticity of second spring 18 to make guard plate 17 and the inseparable laminating of needle tubing 3 outer wall.
When purification work goes on, can be close to each other two sets of rings 12 of placing and connect through magnet 14, can place two sets of rings 12 after the connection is accomplished and arrange the upside of spacing ring 11 in, realize will placing ring 12 and install the inside at cylinder 1, during the appearance of advance, can pour sample solution into the inside of cylinder 1, make sample solution fall to filter screen 13 on, water conservancy diversion through filter screen 13, make sample solution flow down from the inside central point of cylinder 1, avoid sample solution to splash with the contact of cylinder 1 inner wall at the in-process of pouring, guarantee antibody purification effect.
After purification work, ring 12 is placed in the upwards pulling of accessible, can will place ring 12 and take out from 1 inside cylinders, can insert sealed lid 4 this moment to 1 inside cylinders, make the scraper blade 7 that sealed lid 4 downside set up contact with 1 inner wall of cylinder, scraper blade 7 receives blockking of 1 inner wall of cylinder and is close to each other this moment, the articulated mast 9 simultaneous atress of the inner setting of scraper blade 7 this moment, the slider 8 atress that is connected with articulated mast 9 slides in the spout, make two sets of articulated mast 9 atress take place angular variation, elastic deformation takes place for the first spring 10 of connecting between the extrusion articulated mast 9, utilize the elasticity of first spring 10 to make scraper blade 7 closely laminate with 1 inner wall of cylinder, through the sealed lid 4 of reciprocal pulling, the realization is cleared up the remaining viscous solution of 1 inner wall of cylinder, the above does the utility model discloses a whole theory of operation.
It is obvious to a person skilled in the art that the invention is not restricted to details of the above-described exemplary embodiments, but that it can be implemented in other specific forms without departing from the spirit or essential characteristics of the invention. The present embodiments are therefore to be considered in all respects as illustrative and not restrictive, the scope of the invention being indicated by the appended claims rather than by the foregoing description, and all changes which come within the meaning and range of equivalency of the claims are therefore intended to be embraced therein. Any reference sign in a claim should not be construed as limiting the claim concerned.
Claims (6)
1. Plain type antibody purification chromatographic column, including cylinder (1), its characterized in that: the downside of cylinder (1) is provided with control valve (2), and downside fixedly connected with needle tubing (3) of control valve (2), the top of cylinder (1) is provided with sealed lid (4), and the downside vertical connection of sealed lid (4) has connecting rod (5), the fixed cover in bottom of connecting rod (5) has cup jointed sleeve block (6), and the outside of cup block (6) is provided with two sets of scraper blade (7), scraper blade (7) and cup block (6) are close to the upper and lower both ends of one side each other and have seted up the spout, and the inside all blocks of spout have slider (8), one side that slider (8) are close to each other articulates there are two sets of hinge rod (9), and two sets of hinge rod (9) are articulated each other, and are two sets of hinge rod (9) are connected through first spring (10).
2. The simplified antibody purification chromatography column of claim 1, wherein: inner wall fixedly connected with spacing ring (11) of cylinder (1) upside, and the upside of spacing ring (11) has been placed two sets of rings (12) of placing, and two sets of the downside of placing ring (12) all is provided with filter screen (13), and is two sets of place one side difference fixedly connected with magnet (14) that ring (12) are close to each other.
3. The simplified antibody purification chromatography column of claim 1, wherein: the outer side of the column body (1) is movably sleeved with a sliding sleeve (19), and the outer surface of the sliding sleeve (19) is uniformly provided with convex blocks.
4. The simplified antibody purification chromatography column of claim 1, wherein: both ends have montant (15) through bolted connection respectively about control valve (2) downside, and the inside of montant (15) all transversely inserts and is equipped with and insert and establish pole (16), insert one side fixedly connected with guard plate (17) that establish pole (16) are close to each other, and one side that guard plate (17) kept away from each other is connected with second spring (18), the surface of inserting and establishing pole (16) has cup jointed second spring (18).
5. The simplified antibody purification chromatography column of claim 1, wherein: the cross section of the two groups of the scrapers (7) is of an arc structure, and the upper sides of the scrapers (7) are of an inclined structure.
6. The simplified antibody purification chromatography column of claim 2, wherein: two sets of the longitudinal section of filter screen (13) sets up to the arc structure, and is two sets of the convex surface of filter screen (13) is down.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022120698.4U CN213466854U (en) | 2020-09-24 | 2020-09-24 | Simple antibody purification chromatographic column |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022120698.4U CN213466854U (en) | 2020-09-24 | 2020-09-24 | Simple antibody purification chromatographic column |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213466854U true CN213466854U (en) | 2021-06-18 |
Family
ID=76362557
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202022120698.4U Expired - Fee Related CN213466854U (en) | 2020-09-24 | 2020-09-24 | Simple antibody purification chromatographic column |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213466854U (en) |
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2020
- 2020-09-24 CN CN202022120698.4U patent/CN213466854U/en not_active Expired - Fee Related
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| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20210618 |