CN213465106U - Trace liquid metabolite draws storage device - Google Patents
Trace liquid metabolite draws storage device Download PDFInfo
- Publication number
- CN213465106U CN213465106U CN202021691704.5U CN202021691704U CN213465106U CN 213465106 U CN213465106 U CN 213465106U CN 202021691704 U CN202021691704 U CN 202021691704U CN 213465106 U CN213465106 U CN 213465106U
- Authority
- CN
- China
- Prior art keywords
- tube
- extraction
- storage
- sampling
- storage tube
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
Images
Landscapes
- Sampling And Sample Adjustment (AREA)
Abstract
The utility model discloses a trace liquid metabolite draws storage device belongs to medical instrument technical field. It comprises a sampling capillary tube, an extraction tube and a storage tube; the sampling capillary tube consists of a conical funnel part and a capillary tube connected to the bottom of the conical funnel part; the extraction pipe consists of an extraction pipe body and an extraction sealing cover; the storage tube is composed of a storage tube body and a storage sealing cover screwed on the upper opening of the storage tube body. The sampling capillary, the extraction tube and the storage tube are matched to complete three processes of sampling, metabolite centrifugal extraction, sealed storage or sample introduction of the trace liquid, the operation is simple and convenient, the obtained trace liquid and the metabolite thereof cannot cause loss in the operation process, the volume of the extracted trace liquid is reserved to the maximum extent, impurities in an extraction supporting material are prevented from being mixed, the metabolite can be eluted, and the sample pretreatment operation and time are simplified.
Description
Technical Field
The utility model relates to a trace liquid metabolite draws storage device belongs to medical instrument technical field.
Background
The micro liquid of human body such as tears is the only non-invasive sample matrix that can be easily obtained by human body, can reflect certain physiological state of eyes and human body, because tears are small, sample concentration is low, it is difficult to collect and detect, the mass spectrum metabonomics detection has high sensitivity, high flux and high accuracy, has presented great potential in the detection research of small molecule chemical composition in tears, receives more and more attention, the metabonomics flow includes: sample collection, sample pretreatment, metabolite detection, data processing and biological information analysis, wherein the uniformity and the time length of the sample pretreatment have great influence on the detection of the metabolite. And the collection, pretreatment and nitrogen blow-drying processes of the sample need to transfer liquid back and forth, which may pollute the sample and cause great loss, and for the trace liquid which is difficult to collect, the sample volume is insufficient, the metabolite fidelity rate is reduced, the homogeneity among samples is reduced, and the result deviation of the metabolic analysis is large. In addition, in clinic, tears can be collected through Schirmer test paper, polyvinyl alcohol aldehyde sponge and graduated capillary, and after tears are collected through Schirmer test paper or polyvinyl alcohol aldehyde sponge, high-speed centrifugation or methanol elution is usually adopted, but the defects are that the volume of tears permeating into the test paper/sponge is difficult to accurately determine, the recovery rate of small molecular chemical components cannot be ensured, and in addition, plant fibers in the test paper/sponge disturb the presentation of real metabolites. Therefore, the device for extracting and storing the trace liquid metabolite is creatively and developmentally designed according to the long-term tear metabonomics metabolite extraction experience.
Disclosure of Invention
The utility model discloses the technical problem that will solve lies in: provides a micro liquid metabolite extraction and storage device, which solves the problems that the tear cannot be quantitatively sampled and the collection and the storage are completed to the greatest extent and purest degree.
The utility model discloses the technical problem that will solve takes following technical scheme to realize:
a micro liquid metabolite extraction and storage device comprises a sampling capillary tube, an extraction tube and a storage tube;
the sampling capillary tube consists of a conical funnel part and a capillary tube connected to the bottom of the conical funnel part, and sampling scales are arranged on the capillary tube;
the extraction tube comprises an extraction tube body and an extraction sealing cover, a funnel-shaped cavity for containing a sampling capillary tube is arranged in the extraction tube body, the sampling capillary tube is tightly placed in the funnel-shaped cavity, the extraction sealing cover is arranged at the upper opening of the funnel-shaped cavity, a conical connecting part communicated with the lower opening of the funnel-shaped cavity is arranged at the bottom of the extraction tube body, an opening is arranged at the bottom of the conical connecting part, a conical SPE filter matrix is filled in the conical connecting part, the bottom end of the sampling capillary tube is close to the upper surface of the SPE filter matrix, and an external; the SPE filter matrix is a Solid-Phase Extraction (SPE) filter matrix.
The storage tube comprises storage tube body and the sealed lid of spiro union open-ended storage on the storage tube body, and storage tube body open-ended inside and outside is equipped with in-connection screw thread, external screw thread respectively, be equipped with in the storage tube body with toper connecting portion complex bell jar, the bell jar bottom is equipped with spherical reservoir, draw the external connection screw thread of body and the in-connection screw thread spiro union of storage tube body after, toper connecting portion closely support and lean on in the bell jar.
As a preferred example, the storage tube body is made of brown light-resistant material.
Preferably, the extraction pipe body and the extraction sealing cover are integrally formed by plastic materials, and a plastic connecting part is arranged between the extraction pipe body and the extraction sealing cover.
As a preferred example, the outer surface of the extraction tube is provided with a frosted marking area.
As a preferred example, the outer surface of the storage tube is provided with a white mark area.
As a preferred example, the range of the sampling capillary is 30. mu.l.
The using method comprises the following steps:
(1) sampling the micro liquid, taking the tear as an example, sucking the tear of a patient by using a sampling capillary, and reading the volume of the tear through a sampling scale;
(2) extracting trace liquid metabolite, namely putting a sampling capillary tube into a funnel-shaped cavity of an extraction tube, screwing the bottom of the extraction tube into a storage tube body, enabling a conical connecting part to be tightly abutted against a conical groove, enabling the bottom end of the sampling capillary tube to be close to an SPE filter substrate, injecting a small amount of liquid phase into the sampling capillary tube, closing an extraction sealing cover, enabling a frosted marking area on the outer surface of the extraction tube to be capable of marking related marks such as marking sample types, volumes, concentrations and the like, putting an integrated structure formed by the sampling capillary tube, the extraction tube and the storage tube into a centrifuge for centrifugation, enabling the trace liquid to flow out of the sampling capillary tube, filtering the substrate through the SPE;
(3) and storing or feeding trace liquid metabolite, rotationally detaching the extraction tube after centrifugation is finished, connecting the storage sealing cover to the upper opening of the storage tube body in a screwed mode through external threads, and recording the type, volume, concentration and the like of related samples in a white mark area on the outer surface of the storage tube. The sample can be stored in a refrigerator at the temperature of-80 ℃, and the mass spectrum sampling can also be directly carried out after the storage tube is opened.
The utility model has the advantages that:
(1) the sampling capillary tube, the extraction tube and the storage tube are matched to finish three processes of sampling, metabolite centrifugal extraction and sealed storage of trace liquid, the operation is simple and convenient, the obtained trace liquid metabolite cannot cause loss or pollution in the operation process, the volume of the collected trace liquid is reserved to the maximum extent, and impurities in a collection supporting material are prevented from being mixed;
(2) acquiring the volume of the tears through sampling scales on a sampling capillary tube so as to dilute each trace liquid to the same volume percentage concentration before detection;
(3) the spherical liquid storage tank is arranged, so that trace liquid can be collected more intensively;
(4) the storage tube body is made of a brown light-resistant material, so that the influence of illumination on the small molecular components of the tears can be avoided, and the detection result is more accurate;
(5) in the actual sample collection process, the sampling capillary tube can be placed in the combination of the extraction tube and the storage tube for sealed storage only by recording the volume of the sampling capillary tube after collecting the trace liquid, no redundant transfer operation is needed, the sampling capillary tube can be centrifuged when needed, and the loss in trace liquid transfer is reduced;
(6) the SPE at the bottom of the extraction tube filters the setting of the matrix, so that the sample pretreatment operation steps are simple, rapid and simplified, the pretreatment time is shortened, the working efficiency is improved, and the extracted metabolites are easy to store, transport and experiment quality control.
Drawings
Fig. 1 is a schematic structural view of the present invention;
FIG. 2 is a schematic diagram of a sampling capillary;
FIG. 3 is a schematic view of the structure of an extraction tube;
FIG. 4 is a schematic view of the structure of the storage tube;
FIG. 5 is a schematic view of the sample capillary, the extraction tube and the storage tube fixed as an integral structure;
FIG. 6 is a schematic view of the sealed storage tube.
In the figure: sampling capillary 1, taper funnel portion 11, capillary 12, sampling scale 13, extraction tube 2, extraction tube body 21, extraction sealing cover 22, funnel-shaped cavity 23, taper connecting portion 24, SPE filter substrate 25, external connection screw thread 26, plastic connecting portion 27, frosted mark area 28, storage tube 3, storage tube body 31, storage sealing cover 32, internal connection screw thread 33, external screw thread 34, taper groove 35, spherical reservoir 36, white mark area 37.
Detailed Description
In order to make the technical means, the creation features, the achievement purposes and the functions of the present invention easy to understand and understand, the present invention is further explained by combining the following specific drawings.
As shown in fig. 1-6, a device for extracting and storing a micro liquid metabolite, which comprises a sampling capillary 1, an extraction tube 2, and a storage tube 3;
the sampling capillary 1 consists of a conical funnel part 11 and a capillary 12 connected to the bottom of the conical funnel part 11, and the capillary 12 is provided with a sampling scale 13;
the extraction tube 2 is composed of an extraction tube body 21 and an extraction sealing cover 22, a funnel-shaped cavity 23 for containing the sampling capillary tube 1 is arranged in the extraction tube body 21, the sampling capillary tube 1 is tightly placed in the funnel-shaped cavity 23, the extraction sealing cover 22 is arranged at the upper opening of the funnel-shaped cavity 23, a conical connecting part 24 communicated with the lower opening of the funnel-shaped cavity 23 is arranged at the bottom of the extraction tube body 21, an opening is arranged at the bottom of the conical connecting part 24, a conical SPE filter matrix 25 is filled in the conical connecting part 24, the bottom end of the sampling capillary tube 1 is close to the upper surface of the SPE filter matrix 25, and an external;
the storage tube 3 is composed of a storage tube body 31 and a storage sealing cover 32 screwed on the upper opening of the storage tube body 31, inner and outer sides of the upper opening of the storage tube body 31 are respectively provided with inner connecting threads 33 and outer threads 34, a conical groove 35 matched with the conical connecting part 24 is arranged in the storage tube body 31, the bottom of the conical groove 35 is provided with a spherical liquid storage tank 36, and after the outer connecting threads 26 of the extraction tube body 21 are screwed with the inner connecting threads 33 of the storage tube body 31, the conical connecting part 24 is tightly abutted in the conical groove 35.
The storage tube 31 is made of brown light-resistant material.
The extraction pipe body 21 and the extraction sealing cover 22 are integrally formed by plastic materials, and a plastic connecting part 27 is arranged between the extraction pipe body 21 and the extraction sealing cover 22.
The extraction tube 2 is provided with a frosted marking zone 28 on its outer surface.
The outer surface of the storage tube 3 is provided with a white marked area 37.
The range of the sampling capillary 1 was 30. mu.l.
The using method comprises the following steps:
(1) sampling a trace amount of liquid, taking the example of tear, sucking the tear of a patient by using a sampling capillary 1, and reading the volume of the tear through a sampling scale 13;
(2) extracting trace liquid metabolites, namely putting a sampling capillary tube 1 into a funnel-shaped cavity 23 of an extraction tube 2, screwing the bottom of the extraction tube 2 into a storage tube body 31, enabling a conical connecting part 24 to be tightly abutted against a conical groove 35, enabling the bottom end of the sampling capillary tube 1 to be close to an SPE filter substrate 25, injecting a small amount of liquid phase into the sampling capillary tube 1, closing an extraction sealing cover 22, enabling a frosted marking area 28 on the outer surface of the extraction tube 2 to be used for making relevant marks such as marking sample types, volumes, concentrations and the like, putting an integrated structure formed by the sampling capillary tube 1, the extraction tube 2 and the storage tube 3 into a centrifuge for centrifugation, enabling trace liquid to flow out of the sampling capillary tube 1, filtering the substrate 25 through the SPE, and enabling;
(3) and (3) storing or sampling trace liquid metabolites, rotationally detaching the extraction tube 2 after centrifugation is finished, screwing the storage sealing cover 32 on the upper opening of the storage tube body 31 through the external thread 34, and recording related sample types, volumes, concentrations and the like in the white marking area 37 on the outer surface of the storage tube 3. The sample can be stored in a refrigerator at the temperature of minus 80 ℃, and the mass spectrum sampling can also be directly carried out after the storage tube 3 is opened.
The utility model has the advantages that: the sampling capillary tube 1, the extraction tube 2 and the storage tube 3 are matched to finish three processes of sampling, metabolite centrifugal extraction and sealed storage of trace liquid, the operation is simple and convenient, the obtained trace liquid metabolite cannot cause loss or pollution in the operation process, the volume of the collected trace liquid is reserved to the maximum extent, and impurities in a collection supporting material are prevented from being mixed;
acquiring the volume of the tears through a sampling scale 13 on the sampling capillary 1 so as to dilute each trace liquid to the same volume percentage concentration before detection;
the spherical liquid storage tank 36 is arranged, so that trace liquid can be collected more intensively;
the storage tube body 31 is made of a brown light-resistant material, so that the influence of illumination on the small molecular components of the tears can be avoided, and the detection result is more accurate;
in the actual sample collection process, the sampling capillary 1 can be placed in the combination of the extraction tube 2 and the storage tube 3 for sealed storage only by recording the volume of the collected micro liquid, no redundant transfer operation is needed, and the micro liquid is centrifuged when needed, so that the loss in micro liquid transfer is reduced;
the SPE at the bottom of the extraction tube 2 filters the setting of the matrix 25, so that the sample pretreatment operation steps are simple, rapid and simplified, the pretreatment time is shortened, the working efficiency is improved, and the extracted metabolites are easy to store, transport and experiment quality control.
The foregoing illustrates and describes the principles, general features, and advantages of the present invention. It will be understood by those skilled in the art that the present invention is not limited to the above-described embodiments, and that various changes and modifications may be made without departing from the spirit and scope of the invention, and such changes and modifications are intended to be included within the scope of the invention as claimed. The scope of the invention is defined by the appended claims and equivalents thereof.
Claims (6)
1. A micro liquid metabolite extraction and storage device is characterized by comprising a sampling capillary tube, an extraction tube and a storage tube;
the sampling capillary tube consists of a conical funnel part and a capillary tube connected to the bottom of the conical funnel part, and sampling scales are arranged on the capillary tube;
the extraction tube comprises an extraction tube body and an extraction sealing cover, a funnel-shaped cavity for containing a sampling capillary tube is arranged in the extraction tube body, the sampling capillary tube is tightly placed in the funnel-shaped cavity, the extraction sealing cover is arranged at the upper opening of the funnel-shaped cavity, a conical connecting part communicated with the lower opening of the funnel-shaped cavity is arranged at the bottom of the extraction tube body, an opening is arranged at the bottom of the conical connecting part, a conical SPE filter matrix is filled in the conical connecting part, the bottom end of the sampling capillary tube is close to the upper surface of the SPE filter matrix, and an external;
the storage tube comprises storage tube body and the sealed lid of spiro union open-ended storage on the storage tube body, and storage tube body open-ended inside and outside is equipped with in-connection screw thread, external screw thread respectively, be equipped with in the storage tube body with toper connecting portion complex bell jar, the bell jar bottom is equipped with spherical reservoir, draw the external connection screw thread of body and the in-connection screw thread spiro union of storage tube body after, toper connecting portion closely support and lean on in the bell jar.
2. The device for extracting and storing the micro liquid metabolites as claimed in claim 1, wherein the storage tube body is made of a brown light-resistant material.
3. The device for extracting and storing the micro amount of liquid metabolite according to claim 1, wherein the extracting tube and the extracting sealing cap are integrally formed of plastic material, and a plastic connecting portion is provided between the extracting tube and the extracting sealing cap.
4. The device for extracting and storing the micro-amount of the liquid metabolite according to claim 1, wherein the outer surface of the extraction tube is provided with a frosted marking area.
5. The device for extracting and storing the micro-amount of the liquid metabolite according to claim 1, wherein the outer surface of the storage tube is provided with a white mark area.
6. The micro liquid metabolite extraction storage device of claim 1, wherein the range of the sampling capillary is 30 μ l.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021691704.5U CN213465106U (en) | 2020-08-13 | 2020-08-13 | Trace liquid metabolite draws storage device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021691704.5U CN213465106U (en) | 2020-08-13 | 2020-08-13 | Trace liquid metabolite draws storage device |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN213465106U true CN213465106U (en) | 2021-06-18 |
Family
ID=76360584
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202021691704.5U Active CN213465106U (en) | 2020-08-13 | 2020-08-13 | Trace liquid metabolite draws storage device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN213465106U (en) |
-
2020
- 2020-08-13 CN CN202021691704.5U patent/CN213465106U/en active Active
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR101009447B1 (en) | Device for sampling and preprocessing biological fluids and method thereof | |
| US4076419A (en) | Method and apparatus for hematology | |
| CN102735304A (en) | Device and method for detecting gas in liquid | |
| CN213465106U (en) | Trace liquid metabolite draws storage device | |
| CN208968968U (en) | A kind of measurement device of soil mechanical composition | |
| CN109307758A (en) | A kind of white blood cell detection kit | |
| CN208206546U (en) | A kind of sampler convenient for Locale Holding water sample | |
| CN206657020U (en) | A kind of vacuum blood collection diagnosis test tube | |
| CN112461636A (en) | A lipemia sample processing apparatus for conventional detection of blood | |
| SU1272967A3 (en) | Device for collecting and metering liquid | |
| JPH10185912A (en) | Feces examination kit | |
| CN202538808U (en) | Sampling test tube device | |
| CN206074306U (en) | A kind of layering water sampling lever apparatus | |
| CN201083635Y (en) | Disposable vacuum urine-sampling device | |
| CN210109045U (en) | Dasatinib drug concentration detection kit | |
| CN110553871B (en) | Stool sample collecting and detecting integrated electrical measuring device and application method thereof | |
| CN209542626U (en) | A kind of Seperated multiple channel chromatographic apparatus | |
| CN103983766B (en) | A kind of pick-up unit of quantitative collection sample and the method for detection thereof | |
| CN219461216U (en) | Saliva sample collection system | |
| CN108956394A (en) | A kind of measurement device of soil mechanical composition | |
| CN211689061U (en) | Microbial specimen sampler for medical detection | |
| CN206601394U (en) | A kind of Medical Devices of hand-held quick detection biochemical indicator | |
| CN217089303U (en) | High-bottom sample freezing tube for full-automatic immune biochemical analyzer | |
| CN102012421B (en) | Detection device for analyzing analyte in liquid sample | |
| CN204719060U (en) | Novel urine analysis tube |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| GR01 | Patent grant | ||
| GR01 | Patent grant |