CN213416895U - Automatic temperature control device of culture dish - Google Patents
Automatic temperature control device of culture dish Download PDFInfo
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- CN213416895U CN213416895U CN202022066560.0U CN202022066560U CN213416895U CN 213416895 U CN213416895 U CN 213416895U CN 202022066560 U CN202022066560 U CN 202022066560U CN 213416895 U CN213416895 U CN 213416895U
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Abstract
The utility model relates to an automatic temperature control device of culture dish, the device includes: the heating and heat-preserving unit and the control unit; the heating and heat-preserving unit comprises a temperature-controlling shell, a heating sheet and a temperature-measuring probe; an assembly groove is formed in the center of the bottom of the inner side of the temperature control shell and used for fixing the heating sheet; the side wall of the inner side of the temperature control shell is provided with a positioning step for placing a culture dish; the side wall of the inner side of the temperature control shell is also provided with a fixing hole for fixing the temperature measuring probe; the temperature measuring probe is electrically connected with the control unit; still be provided with the recess on the lateral wall of accuse temperature casing for fixed connection line, connecting wire are used for the electricity between heating plate and the control unit to be connected, the device can provide the temperature environment the same with the culture environment for the cell in the observation, maintains the activity of whole observation in-process cell, guarantees the accuracy of testing result, and in addition, the device has the temperature stability, and the temperature field is even, the characteristics that the controllability is high, and whole easy operation, practicality are strong.
Description
Technical Field
The utility model relates to a biological test's technical field especially relates to an automatic temperature regulating device of culture dish.
Background
Optical microscopy is a common sample viewing tool in biological experiments. With the development of the technology, high-end microscopes combining the photoelectronic technology, such as confocal microscopes, are more and more widely applied in life sciences. Such microscope systems have specific requirements on the ambient temperature, for example 22 ± 3 ℃. Therefore, the ambient temperature at which the microscope operates is greatly different from the normal culture temperature of the cells, e.g., 37 ℃. At lower temperatures, the physiological state of the cells may differ greatly from that of cells cultured at normal temperature at 37 ℃, which may distort the detection results. Therefore, it is important to provide a constant temperature of 37 ℃ for the cells under microscopic observation.
SUMMERY OF THE UTILITY MODEL
An object of the utility model is to provide an automatic temperature regulating device of culture dish for the cell in the observation provides the temperature environment the same with the culture environment, maintains the activity of whole observation in-process cell, guarantees the accuracy of testing result.
In order to achieve the above object, the present application provides an automatic temperature control device for a culture dish, comprising: the heating and heat-preserving unit and the control unit; the heating and heat-preserving unit comprises a temperature control shell, a heating sheet and a temperature measuring probe;
an assembly groove is formed in the center of the bottom of the inner side of the temperature control shell and used for fixing the heating sheet;
the side wall of the inner side of the temperature control shell is provided with a positioning step, and the positioning step is used for placing a culture dish;
the side wall of the inner side of the temperature control shell is also provided with a fixing hole, and the fixing hole is used for fixing the temperature measuring probe;
the temperature measuring probe is electrically connected with the control unit;
the lateral wall of the temperature control shell is also provided with a groove, the groove is used for fixing a connecting wire, and the connecting wire is used for electrically connecting the heating plate and the control unit.
Furthermore, the side wall and the bottom of the temperature control shell are both of a double-layer structure, and a communicated hollow cavity is arranged in the middle of the double-layer structure;
the double-layer structure is also provided with an air exhaust passage communicated with the hollow cavity.
Further, the positioning steps are two-stage steps and comprise a first step and a second step;
the height between the first step and the second step is equal to the height of the culture dish;
the second step is flush with the opening of the temperature control shell;
the upper portion of the second step is provided with a threaded hole, the threaded hole is used for screwing in a screw, and the screw and the pressing sheet are matched and fixedly placed on the culture dish on the first step.
Further, a cup-shaped temperature control cavity is formed in the inner side of the temperature control shell and is used for being filled with liquid media.
Furthermore, the control unit comprises a heating regulation module, a microcontroller, a temperature signal conditioning module and a temperature display setting module;
the heating adjusting module is respectively connected with the heating sheet and the microcontroller;
the temperature signal conditioning module is respectively connected with the microcontroller and the temperature measuring probe;
the temperature display setting module is connected with the microcontroller.
Further, the heating regulation module comprises a heating driving module and a voltage regulation module;
the heating driving module is respectively connected with the heating sheet and the microcontroller;
the voltage regulation module is respectively connected with the heating driving module and the microcontroller.
Furthermore, the temperature display setting module is realized through a liquid crystal display and a jog key switch.
Furthermore, the heating plate and the temperature probe are both provided with waterproof structures.
Therefore, according to the technical scheme provided by the application, the culture dish is heated by using the liquid medium, and the culture dish has the functions of real-time temperature detection and closed-loop regulation control, and is stable in temperature, uniform in temperature field and high in controllability; the culture dish positioning and fixing structure is provided, the operation is simple, and the practicability is high.
Furthermore, the culture dish can be conveniently installed through the positioning step arranged in the temperature control shell, and the culture dish can be placed into the automatic temperature control device, so that the culture dish has the characteristics of small size and small occupied space, can be easily placed in the narrow observation space of the microscope, and has good universal performance.
It should be noted that the positioning step arranged in the temperature control shell can also reduce the contact area between the culture dish and the side wall at the inner side of the temperature control shell, and ensure that the culture dish cannot have uneven temperature between the side surface of the culture dish and the ground due to the heat conduction of the side wall. In addition, the temperature control shell is of a hollow structure and can be vacuumized, and the heat preservation effect of the temperature control shell can be further improved.
Further, the inboard of accuse temperature casing forms cupped accuse temperature chamber, and accuse temperature chamber is used for filling liquid medium for the culture dish soaks liquid medium, and the heating plate can heat this liquid medium, provides the control by temperature change environment by liquid medium to the culture dish again, guarantees the stability and the homogeneity of culture dish temperature.
In addition, the temperature measuring probe can be arranged at the position which can be immersed in the liquid medium in the same way, and due to the good heat conductivity of the liquid medium, the real-time temperature measured by the temperature measuring probe can be ensured to represent the real-time temperature of the culture dish, and the measuring accuracy is ensured.
Drawings
Fig. 1 is a schematic overall view of an automatic temperature control device for a culture dish according to an embodiment of the present invention;
fig. 2 is a schematic structural view of a temperature control housing according to an embodiment of the present invention;
fig. 3 is a top view of a temperature control housing according to an embodiment of the present invention.
In the figure, 1, a heating and heat-preserving unit; 1.1, controlling the temperature of the shell; 1.2, heating plates; 1.3, a liquid medium; 1.4, a culture dish; 1.5, a temperature measuring probe; 2. a control unit; 2.1, heating a driving module; 2.2, a voltage regulation module; 2.3, a microcontroller; 2.4, a temperature display setting module; 2.5, a temperature signal conditioning module; 1.11, side walls; 1.12, fixing holes; 1.13, assembling grooves; 1.14, a groove; 1.15, a threaded hole; 1.16, positioning steps; 1.17, an air exhaust channel.
Detailed Description
The following examples are intended to illustrate the invention, but are not intended to limit the scope of the invention.
Fig. 1 is a schematic overall view of an automatic temperature control device for a culture dish according to an embodiment of the present invention; fig. 2 is a schematic structural view of a temperature control housing according to an embodiment of the present invention; fig. 3 is a top view of a temperature control housing according to an embodiment of the present invention.
The automatic temperature control device of culture dish that this embodiment provided can be used for installing the culture dish to place under the microscope, for the cell in the culture dish under the observation provides the temperature environment the same with culture environment, maintain the activity of whole observation in-process cell.
Referring to fig. 1 to 3, the automatic temperature control device includes: a heating and heat-preserving unit 1 and a control unit 2; wherein, the heating and heat-preserving unit 1 comprises a temperature control shell 1.1, a heating sheet 1.2 and a temperature measuring probe 1.5;
an assembly groove 1.13 is formed in the center of the bottom of the inner side of the temperature control shell 1.1, and the assembly groove 1.13 is used for fixing the heating plate 1.2;
a positioning step 1.16 is arranged on the side wall 1.11 at the inner side of the temperature control shell 1.1, and the positioning step 1.16 is used for placing a culture dish 1.4;
a fixing hole 1.12 is also arranged on the side wall 1.11 at the inner side of the temperature control shell 1.1, and the fixing hole 1.12 is used for fixing the temperature measuring probe 1.5;
wherein, the temperature probe 1.5 can use a resistance type temperature sensor, such as PT 1000.
The temperature measuring probe 1.5 is electrically connected with the control unit 2;
a groove 1.14 is further formed in the side wall 1.11 of the temperature control housing 1.1, the groove 1.14 is used for fixing a connecting wire, and the connecting wire is used for electrically connecting the heating sheet 1.2 and the control unit 2.
In use, the culture dish 1.4 can be placed on the positioning step 1.16 of the temperature-controlled housing 1.1 to fix the culture dish 1.4 in the automatic temperature-controlling device, and further, the automatic temperature-controlling device with the culture dish 1.4 fixed is placed under a microscope. The temperature probe 1.5 in the automatic temperature control device can measure the real-time temperature of the culture dish 1.4 and send the real-time temperature to the control unit 2. The control unit 2 can control the heating sheet 1.2 to perform heating operation according to the real-time temperature, has a real-time temperature detection-adjustment closed-loop control function, and ensures that the real-time temperature of the culture dish 1.4 is kept in a constant temperature state, such as 37 ℃, so that a temperature environment which is the same as the culture environment can be provided for the cells in the culture dish 1.4 under observation, and the activity of the cells in the whole observation process can be maintained.
In addition, the positioning step 1.16 arranged in the temperature control shell 1.1 can facilitate the installation of the culture dish 1.4, and the culture dish 1.4 can be placed in the automatic temperature control device, so that the temperature control device has the characteristic of small occupied space and can be easily placed in the narrow observation space of a microscope.
It should be noted that the positioning step 1.16 arranged in the temperature control housing 1.1 can also reduce the contact area between the culture dish 1.4 and the side wall 1.11 at the inner side of the temperature control housing 1.1, and ensure that the culture dish 1.4 does not have the problem of uneven temperature between the side surface of the culture dish 1.4 and the ground due to the heat conduction of the side wall 1.11.
Further, the inner side of the temperature-controlled housing 1.1 forms a cup-shaped temperature-controlled chamber for filling with the liquid medium 1.3, so that the culture dish 1.4 is immersed in the liquid medium 1.3.
Specifically, in use, the culture dish 1.4 can be placed on the positioning step 1.16 of the temperature-controlled housing 1.1, and a liquid medium 1.3, such as water, is injected into the temperature-controlled cavity, so that the culture dish 1.4 is immersed in the liquid medium 1.3. Further, the heating plate 1.2 can heat the liquid medium 1.3, and then the liquid medium 1.3 provides a temperature control environment for the culture dish 1.4, so that the stability and uniformity of the temperature of the culture dish 1.4 are ensured.
In addition, the temperature measuring probe 1.5 can be arranged at the position which can be immersed in the liquid medium 1.3 in the same way, and due to the good heat conductivity of the liquid medium 1.3, the real-time temperature measured by the temperature measuring probe 1.5 can be ensured to represent the real-time temperature of the culture dish 1.4, and the measurement accuracy is ensured.
On the basis of the technical scheme, the side wall 1.11 and the bottom of the temperature control shell 1.1 can be set to be of a double-layer structure, and the middle of the double-layer structure is a communicated hollow cavity.
In an embodiment, the double-layer structure may also be provided with an air evacuation channel 1.17 communicating with the hollow cavity. When in use, the hollow cavity can be vacuumized through the air exhaust channel 1.17, so that the heat preservation function of the temperature control shell 1.1 is enhanced.
Of course, in another embodiment, the hollow cavity may also be directly provided as a sealed vacuum cavity.
On the basis of the technical scheme, referring to fig. 2 and 3, the positioning step 1.16 can be set to be a two-step, including a first step and a second step; the height between the first step and the second step is equal to the height of the culture dish 1.4; the second step is flush with the opening of the temperature control shell 1.1; the upper portion of second step is provided with screw hole 1.15, and screw hole 1.15 is used for the screw in, and this screw and preforming cooperation are fixed and are placed culture dish 1.4 on first step.
On one hand, the height between the first step and the second step is equal to the height of the culture dish 1.4, so that the heating sheet 1.2 and the culture dish 1.4 can have a certain space and can contain enough liquid medium 1.3, but the heating sheet 1.2 is not directly contacted with the culture dish 1.4, and the uniformity of heating the culture dish 1.4 is ensured; on the other hand, the height of the culture dish 1.4 can be ensured to be just flush with the opening of the temperature control shell 1.1, so that the liquid medium 1.3 injected into the temperature control cavity can not flow into the culture dish 1.4.
On the basis of the above technical solution, referring to fig. 1, the control unit 2 may include a heating adjustment module, a microcontroller 2.3, a temperature signal conditioning module 2.5, and a temperature display setting module 2.4; the heating adjusting module is respectively connected with the heating sheet 1.2 and the microcontroller 2.3; the temperature signal conditioning module 2.5 is respectively connected with the microcontroller 2.3 and the temperature measuring probe 1.5; the temperature display setting module 2.4 is connected with the microcontroller 2.3.
Further, the control unit 2 may also include a power management module that provides the supply voltage required by the control unit 2.
In one embodiment, the temperature display setting module 2.4 can be implemented by using a liquid crystal display and a jog key switch.
Exemplarily, the temperature display setting module 2.4 can be realized by using a 12864 liquid crystal screen and a jog key switch, and the 12864 liquid crystal screen can display the temperature value measured by the temperature measuring probe 1.5 in real time under the control of the microcontroller 2.3. The temperature value maintained by the culture dish 1.4, e.g. 37 c, can also be set by clicking a key switch.
In one embodiment, the temperature signal conditioning module 2.5 may use a Wheatstone bridge to measure the resistance of the temperature probe 1.5 with temperature. Specifically, when the real-time temperature of the culture dish 1.4 changes, the resistance value of the temperature probe 1.5 (such as a resistance temperature sensor, PT1000) changes, and the temperature signal conditioning module 2.5 can convert the resistance value change into a voltage signal that can be measured by the microcontroller 2.3. Further, the microcontroller 2.3 may convert the voltage signal into a corresponding temperature, thereby providing a basis for controlling the heating operation of the heating plate 1.2.
Specifically, the temperature measuring probe 1.5 and the temperature signal conditioning module 2.5 measure the temperature of the controlled liquid medium 1.3 (the same as the temperature of the culture dish 1.4) in real time, the measured temperature is fed back to the microcontroller 2.3, the microcontroller 2.3 can control the heating power of the heating plate 1.2 according to the automatic heating temperature control programming logic, wherein the control of the heating power of the heating plate 1.2 can include the adjustment of the heating duty ratio and the supply voltage of the heating plate 1.2.
In one embodiment, an automatic heating temperature control programming logic may comprise the steps of:
a: actually measuring the temperature difference delta T between the temperature of the liquid medium 1.3 and the set temperature, the temperature difference threshold delta T1, the temperature difference threshold delta T2, and delta T2< delta T1;
b: when the delta T is larger than the delta T1, a heating mode with the heating time duty ratio of 1 and the maximum power supply voltage is adopted; when the delta T1 is larger than the delta T2, a heating mode of reducing the duty ratio of heating time and the maximum power supply voltage is adopted; when Δ T2> Δ T, a heating method is employed in which the heating time duty ratio is reduced and the power supply voltage is reduced.
As an alternative embodiment, the microcontroller 2.3 may control the length of time the heating plate 1.2 is heated in a square wave duty cycle.
The formula for duty cycle adjustment of the heating sheet 1.2 can be as follows:
in the formula, Δ T is the temperature difference between the actually measured temperature of the liquid medium 1.3 and the set temperature, T is the heating period, a is the set parameter, and y is the heating time within the heating period T.
On the basis of the technical scheme, the heating adjusting module can use an adjustable voltage chip to match with a digital adjustable resistance chip to adjust the power supply voltage on the heating sheet 1.2; the voltage may also be regulated using Pulse Width Modulation (PWM).
The adjustable voltage chip can adjust the resistance ratio of the feedback pin of the chip to realize the purpose of adjustable output voltage; the 'digital adjustable resistance chip' can set the resistance value of the output through the I2C protocol. The microcontroller 2.3 sets the output resistance value of the digital adjustable resistance chip through an I2C protocol, and changes the resistance ratio on the feedback pin of the adjustable voltage chip, thereby achieving the purpose of controlling the power supply voltage of the heating plate 1.2; under the condition of the unchanged adjustment of the duty ratio of the heating sheet 1.2, the power supply voltage on the heating sheet 1.2 is reduced, the heating power is reduced, and the power supply voltage on the heating sheet 1.2 is increased, and the heating power is increased.
The voltage regulation of the heating plate 1.2 can be expressed as follows:
Vout＝Vref*(1+R2/R1)
in the formula, R1 is a constant value resistor 1K omega (1% precision) and is connected between a feedback pin of the adjustable voltage chip and the ground; r2 represents the resistance of the digital adjustable resistance chip in the voltage adjustment module 2.2, set via I2C, that can be connected between the feedback pin and the voltage output pin of the adjustable voltage chip; vref is typically 1.23V.
In an embodiment, referring to fig. 1, the heating regulation module comprises a heating driving module 2.1 and a voltage regulation module 2.2; the heating driving module 2.1 is respectively connected with the heating sheet 1.2 and the microcontroller 2.3; the voltage regulation module 2.2 is connected with the heating driving module 2.1 and the microcontroller 2.3 respectively.
In particular, the heating driving module 2.1 may use a field effect transistor and an optical coupler isolator. In particular, a field effect transistor is connected between the heating film and the voltage regulating module 2.2. The grid of the field effect transistor can be connected to the microcontroller 2.3 through an optical coupler, and on-off control of power supply of the heating film is achieved under the control of the microcontroller 2.3. The field effect tube has extremely low voltage drop, so that the power supply voltage passing through the voltage regulating module 2.2 can be ensured to act on the heating film for the most part.
On the basis of the above technical scheme, both the heating sheet 1.2 and the temperature probe 1.5 can be subjected to waterproof treatment, and in an embodiment, both the heating sheet 1.2 and the temperature probe 1.5 can be provided with waterproof structures, such as waterproof films and the like. Specifically, the waterproof ratings of the heating sheet 1.2 and the temperature probe 1.5 may be set to the standard of IP 68.
Although the invention has been described in detail in the foregoing by way of general description, specific embodiments and experiments, it will be apparent to those skilled in the art that certain modifications and improvements may be made thereto based on the invention. Therefore, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (8)
1. An automatic temperature control device of culture dish, its characterized in that includes: the heating and heat-preserving unit and the control unit; the heating and heat-preserving unit comprises a temperature control shell, a heating sheet and a temperature measuring probe;
an assembly groove is formed in the center of the bottom of the inner side of the temperature control shell and used for fixing the heating sheet;
the side wall of the inner side of the temperature control shell is provided with a positioning step, and the positioning step is used for placing a culture dish;
the side wall of the inner side of the temperature control shell is also provided with a fixing hole, and the fixing hole is used for fixing the temperature measuring probe;
the temperature measuring probe is electrically connected with the control unit;
the lateral wall of the temperature control shell is also provided with a groove, the groove is used for fixing a connecting wire, and the connecting wire is used for electrically connecting the heating plate and the control unit.
2. The device according to claim 1, wherein the side wall and the bottom of the temperature control shell are both of a double-layer structure, and a communicated hollow cavity is arranged in the middle of the double-layer structure;
the double-layer structure is also provided with an air exhaust passage communicated with the hollow cavity.
3. The apparatus of claim 1, wherein the positioning step is a two-step comprising a first step and a second step;
the height between the first step and the second step is equal to the height of the culture dish;
the second step is flush with the opening of the temperature control shell;
the upper portion of the second step is provided with a threaded hole, the threaded hole is used for screwing in a screw, and the screw and the pressing sheet are matched and fixedly placed on the culture dish on the first step.
4. The device according to claim 1, wherein the inner side of the temperature-controlled housing forms a cup-shaped temperature-controlled chamber for filling with a liquid medium.
5. The device of claim 1, wherein the control unit comprises a heating adjustment module, a microcontroller, a temperature signal conditioning module, and a temperature display setting module;
the heating adjusting module is respectively connected with the heating sheet and the microcontroller;
the temperature signal conditioning module is respectively connected with the microcontroller and the temperature measuring probe;
the temperature display setting module is connected with the microcontroller.
6. The apparatus of claim 5, wherein the heating regulation module comprises a heating drive module and a voltage regulation module;
the heating driving module is respectively connected with the heating sheet and the microcontroller;
the voltage regulation module is respectively connected with the heating driving module and the microcontroller.
7. The device of claim 5, wherein the temperature display setting module is implemented by a liquid crystal display and a jog key switch.
8. The device of claim 1, wherein the heating plate and the temperature probe are provided with waterproof structures.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022066560.0U CN213416895U (en) | 2020-09-18 | 2020-09-18 | Automatic temperature control device of culture dish |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202022066560.0U CN213416895U (en) | 2020-09-18 | 2020-09-18 | Automatic temperature control device of culture dish |
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| Publication Number | Publication Date |
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| CN213416895U true CN213416895U (en) | 2021-06-11 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN202022066560.0U Active CN213416895U (en) | 2020-09-18 | 2020-09-18 | Automatic temperature control device of culture dish |
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| CN (1) | CN213416895U (en) |
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