CN213404656U - Sperm freezing device - Google Patents
Sperm freezing device Download PDFInfo
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- CN213404656U CN213404656U CN202021348283.6U CN202021348283U CN213404656U CN 213404656 U CN213404656 U CN 213404656U CN 202021348283 U CN202021348283 U CN 202021348283U CN 213404656 U CN213404656 U CN 213404656U
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Abstract
The utility model relates to the technical field of sperm preservation, and discloses a sperm freezing device, which comprises a grid hanging basket, a grid interlayer, a suspension arm, an inserted link, a cover plate and a positioning component; the davit is opened has evenly distributed's scale hole, the davit is rotationally installed in the net hanging flower basket, the inserted bar cooperatees with the scale hole of davit, the length of inserted bar is greater than the external diameter of liquid nitrogen container mouth, the net interlayer sets up in the net hanging flower basket, locating component's one end is installed in the middle part of net hanging flower basket bottom, locating component's the other end passes the net interlayer and extends to the open end of net hanging flower basket, apron position is adjustably installed in locating component, and its beneficial effect lies in: the distance between the bottom of the grid hanging basket and the liquid nitrogen surface is conveniently adjusted by arranging the suspension arm, and the volatile steam of liquid nitrogen is blocked by arranging the cover plate, so that the ampoule filled with the sperm suspension is rapidly and uniformly cooled and frozen, the freezing effect of the sperm suspension is improved, and the quality of freeze-dried sperm is improved.
Description
Technical Field
The utility model relates to a sperm preserve technical field, concretely relates to sperm freezing device.
Background
In the technical field of sperm preservation, a sperm freeze-drying method utilizes certain conditions of low temperature, vacuum and negative pressure to act on sperms, so that the sperms are in a completely dehydrated and dried state, biochemical and metabolic activities of the sperms are completely stopped, and the sperms can be stored at a normal temperature.
During the lyophilization preservation process, the sperm suspension needs to undergo: adding a freeze-drying protective agent; freezing the sperm at cryogenic temperature; thirdly, in the frozen state, the water of the sperms is directly sublimated from the frozen solid state by means of the negative pressure generated by the vacuum pump, so that the sperms are completely dehydrated, and the vacuum freeze drying is realized; freeze-drying sperm and storing at normal temperature; fifthly, when the freeze-dried sperms need to be used, adding water to restore the state of the sperm suspension.
In the third step of the freeze-drying preservation process, the container needing to be subpackaged with the sperm suspension is wide-mouthed, so as to be beneficial to freezing and rapid sublimation, and ensure that the sperm is rapidly and completely dehydrated and dried; in the fifth step of the process, the container is wide-mouthed to facilitate rapid addition of water to bring the sperm back into suspension. Thus, sperm lyophilization requires the use of a wide-mouth container to hold the sperm suspension. However, the wide-mouth container (such as the special low-temperature ampoule shown in the attached drawing) is not easy to be cooled and frozen quickly and uniformly in the second step of the process because the container has a large volume (2 ml). Therefore, there is a need to design a sperm freezing device that can be used in conjunction with sperm lyophilization to rapidly and uniformly perform freezing.
SUMMERY OF THE UTILITY MODEL
The utility model aims at overcoming the defects existing in the prior art and providing a sperm freezing device which is matched with a sperm freeze-drying method and can be quickly and uniformly cooled and frozen.
The purpose of the utility model is realized through the following technical scheme: the device comprises a grid hanging basket, a grid interlayer, a suspension arm, an inserted bar, a cover plate and a positioning component; the davit is opened has evenly distributed's scale hole, the davit is rotationally installed in the net hanging flower basket, the inserted bar cooperatees with the scale hole of davit, the length of inserted bar is greater than the external diameter of liquid nitrogen container mouth, the net interlayer sets up in the net hanging flower basket, and the ampoule is placed in the net hanging flower basket through the net interlayer, locating component's one end is installed in the middle part of net hanging flower basket bottom, locating component's the other end passes the net interlayer and extends to the open end of net hanging flower basket, apron position is adjustably installed in locating component to be located the top of ampoule.
Further, the positioning assembly comprises a screw rod, a fixing nut and a positioning nut; the lead screw passes through fixation nut to be installed in the central authorities of net hanging flower basket bottom, the other end of lead screw extends to the open end of net hanging flower basket, positioning nut rotatably installs the other end in the lead screw, the apron is placed on positioning nut.
Furthermore, a round hole is formed in the center of the cover plate, bent lugs are evenly arranged on the upper end face of the cover plate, and the cover plate is placed on the positioning screw cap through the round hole.
Furthermore, the number of the bent lugs is three, and the three bent lugs are distributed on the upper end face of the cover plate in an isosceles triangle shape.
Further, the suspension arm comprises two vertical rods and a cross rod; the two vertical rods are provided with scale holes which are uniformly distributed along the length direction, the interval between every two scale holes is 0.5cm, one ends of the two vertical rods are respectively and rotatably arranged on the two sides of the grid hanging basket, and the cross rod is connected with the other ends of the two vertical rods.
Further, the length of the vertical rods is 30cm, and the interval between the two vertical rods is 8 cm.
Furthermore, the diameter of the grid hanging basket is 8cm, and the height of the grid hanging basket is 10 cm; the bottom and the periphery of the grid hanging basket are grids woven by thin copper wires, and the size of each grid is 0.7cm x 0.7 cm.
Furthermore, 2cm of interval is arranged between the grid interlayer and the bottom of the grid hanging basket, and the grid interlayer is formed by 1.5 cm-1.5 cm of grids woven by thin copper wires.
Furthermore, the cover plate is made of metal materials, the diameter of the cover plate is 7cm, the thickness of the cover plate is less than 0.1cm, and the diameter of a central round hole of the cover plate is 0.7 cm.
Compared with the prior art, the utility model have following advantage:
1. the utility model discloses in, through be equipped with the scale hole at a distance of 0.5cm on the davit, the inserted bar passes behind the scale hole, and horizontal putting is in the liquid nitrogen jar mouth, and the net hanging flower basket suspends in midair in the liquid nitrogen jar. The inserted bar can rise or fall the net hanging flower basket through passing through different scale holes to adjust the distance between the bottom of the hanging flower basket and the liquid nitrogen surface, keep the distance at 1cm (about-150 ℃), make the net hanging flower basket be at the cryogenic temperature on the liquid nitrogen surface, conveniently utilize the cryogenic temperature of liquid nitrogen steam, freeze the sperm suspension in the ampoule in the net hanging flower basket, and, through rising or falling the net hanging flower basket, to the different liquid nitrogen storage volume in the liquid nitrogen tank, can implement the operation of freezing, do not receive the influence of the liquid nitrogen storage volume in the liquid nitrogen tank. The utility model discloses a still set up locating component and apron in the net hanging flower basket, the apron is placed in the top 0.5cm department of ampoule through locating component, blocks the liquid nitrogen through the apron and volatilizees the steam that rises, forms the local cryrogenic temperature of ampoule fast in the net hanging flower basket, makes the whole ampoule that is equipped with the sperm suspension cool down fast and freeze uniformly to improve the freezing effect of sperm suspension, be favorable to improving the quality of freeze-dried sperm.
2. The utility model provides a locating component is equipped with positioning nut including fixed setting at the lead screw of net hanging flower basket bottom central authorities on the lead screw, and rotatory positioning nut can rise or reduce positioning nut to the height of adjustment positioning nut on the lead screw makes the apron keep flat on positioning nut back, can and the ampoule top between keep 0.5 cm's distance, through the height of adjustment positioning nut at the lead screw like this, facilitates the ampoule of using different specification models and loads the sperm suspension. The cover plate is provided with 3 small bent lugs distributed in an isosceles triangle shape, so that the cover plate can be placed in a balanced manner, and the bent lugs can be conveniently clamped by tweezers to place the book blocking cover plate into a hanging basket and take out the book blocking cover plate.
3. The utility model discloses a be equipped with the net interlayer in the hanging flower basket, make things convenient for the ampoule to pass through the vertical placing of net interlayer, can form ice form solid-state thing in the ampoule lower part when making the sperm suspension freeze, can not take place ampoule and violently put and make the sperm suspension freeze at the ampoule opening part, when having avoided follow-up ampoule freeze-drying of opening, the ampoule opening part freezes and influences the operation and causes the sperm to lose and pollute. The grid interlayer is a grid of 1.5cm by 1.5cm, and a large-capacity (2ml) wide-caliber ampoule is convenient to use for subpackaging the sperm suspension. The use of the ampoule with large capacity and wide caliber is beneficial to rapid sublimation of water in the vacuum drying process and rapid addition of water during rehydration to restore the volume of sperm suspension before lyophilization.
Drawings
The accompanying drawings, which are incorporated in and constitute a part of this application, illustrate embodiments of the invention and, together with the description, serve to explain the invention and not to limit the invention. In the drawings:
fig. 1 shows a schematic structural view of a sperm freezing apparatus according to the present invention.
In the figure, 1 is a grid hanging basket; 2 is a grid interlayer; 3 is a cover plate; 4 is a scale hole; 5 is a screw rod; 6 is a fixing nut; 7 is a positioning screw cap; 8 is a bent lug; 9 is a rotary joint; 10 is an ampoule; 11 is sperm suspension; 12 is a vertical rod; 13 is a cross bar; 14 is an insert rod.
Detailed Description
The present invention will be further explained with reference to the drawings and examples.
The sperm freezing device shown in fig. 1 comprises a grid hanging basket 1, a grid interlayer 2, a suspension arm, an inserted rod 14, a cover plate 3 and a positioning component; the davit is opened scale hole 4 that has evenly distributed, the davit is rotationally installed in net hanging flower basket 1, inserted bar 14 cooperatees with the scale hole 4 of davit, the length of inserted bar 14 is greater than the external diameter of liquid nitrogen tank mouth, net interlayer 2 sets up in net hanging flower basket 1, and ampoule 10 passes through the vertical place in the net hanging flower basket of net interlayer 2, locating component's one end is installed in the middle part of 1 bottom of net hanging flower basket, locating component's the other end passes and extends to the open end of net hanging flower basket 1 behind net interlayer 2, 3 adjustable adjustablely install in locating component in apron to be located ampoule 10's top. The grid hanging basket 1 is placed in a liquid nitrogen tank, and the inserted rod 14 is transversely placed at the opening of the liquid nitrogen tank after passing through the scale hole 4. The grid basket 1 can be raised or lowered by inserting the inserted rods 14 through the different scale holes 4, thereby adjusting the distance between the bottom of the grid basket 1 and the liquid nitrogen surface to keep the distance between the grid basket 1 and the liquid nitrogen surface at 1cm (about-150 ℃). Freezing and freezing the sperm suspension contained in the ampoule 10 in the grid hanging basket 1 by utilizing the cryogenic temperature of liquid nitrogen steam on the liquid nitrogen surface. The cover plate 3 is arranged above the ampoule in an adjustable manner through the positioning assembly and is used for blocking vapor generated by volatilization and rise of liquid nitrogen, and the local cryogenic temperature of the ampoule is quickly formed in the grid hanging basket 1, so that the whole ampoule 10 filled with the sperm suspension 11 is quickly and uniformly cooled and frozen. This device cooperation sperm freeze-drying method uses, through setting up davit and the height-adjustable apron that has the scale mark hole, can the position of ampoule 10 in the liquid nitrogen tank that is equipped with sperm suspension 11 of quick adjustment to and form local cryogenic temperature fast above ampoule 10, make the whole ampoule 10 that is equipped with sperm suspension cool down and freeze fast evenly.
The positioning assembly comprises a lead screw 5, a fixing nut 6 and a positioning nut 7; the long 8cm of lead screw 5, lead screw 5 is installed in the central authorities of 1 bottom of net hanging flower basket through fixation nut 6, the other end of lead screw 5 extends to the open end of net hanging flower basket 1, fixation nut 7 rotatably installs in the other end of lead screw 5, the round hole is seted up in the central authorities of apron 3, the up end of apron 3 evenly is provided with curved ear 8, apron 8 places on fixation nut 7 through the round hole. The center of the bottom of the grid hanging basket 1 is screwed with a screw rod 5 through a fixing nut 6; the other end of the lead screw 5 is screwed into a positioning nut 7; by rotating the positioning nut 7, the positioning nut 7 can be raised or lowered, so that the distance from the positioning nut 7 to the bottom of the grid basket 1 is adjusted, and after the cover plate passes through the screw rod 5 and is flatly placed on the positioning nut 7, the distance between the cover plate and the top of the ampoule 10 can be kept to be 0.5 cm. By adjusting the height of the positioning screw cap 7, ampoules 10 of different specifications and models can be conveniently used for loading sperm suspension
The number of the bent lugs 8 is three, and the three bent lugs 8 are distributed on the upper end surface of the cover plate 3 in an isosceles triangle shape. Three lugs 8 are distributed in an isosceles triangle shape and used for improving the placing stability of the cover plate 3 and facilitating the clamping of the lugs 8 by tweezers to place or take out the cover plate.
The suspension arm comprises two vertical rods 12 and a cross rod 13; the two vertical rods 12 are provided with scale holes 4 which are uniformly distributed along the length direction, the interval between every two scale holes 4 is 0.5cm, one ends of the two vertical rods 12 are respectively and rotatably arranged on two sides of the grid hanging basket 1, and the cross rod 13 is connected with the other ends of the two vertical rods 12. Two vertical rods 12 are rotatably arranged at two sides of the grid hanging basket 1 through rotating joints 9. The inserted rods 14 penetrate through different scale holes 4, and the distance between the bottom of the grid basket 1 and the liquid nitrogen surface can be adjusted. Because liquid nitrogen evaporation can produce different cryrogenic temperature gradients on the liquid nitrogen face, so, different height on the liquid nitrogen face has different cryrogenic temperatures promptly, utilizes different cryrogenic temperatures to implement freezing to the mirror suspension 11 in the ampoule in the net hanging flower basket and freezes.
The diameter of the grid hanging basket 1 is 8cm, and the height of the grid hanging basket 1 is 10 cm; the bottom and the periphery of the grid hanging basket 1 are all grids woven by thin copper wires, and the size of each grid is 0.7cm by 0.7 cm. Through setting up the net, be favorable to liquid nitrogen steam circulation.
The length of the vertical rods 12 is 30cm, the interval between two vertical rods 12 is 8cm, and the interval between the vertical rods 12 corresponds to the diameter of the grid hanging basket 1.
The grid interlayer 2 is 2cm away from the bottom of the grid hanging basket 1, and the grid interlayer 2 is formed by 1.5cm grids formed by weaving thin copper wires. Through setting up the net for the ampoule is vertical to be placed in the hanging flower basket.
The cover plate 3 is made of metal materials, the diameter of the cover plate 3 is 7cm, the thickness of the cover plate 3 is less than 0.1cm, and the diameter of a central round hole of the cover plate 3 is 0.7 cm. The cover plate 3 is placed flat on the positioning screw cap 7 through the circular hole.
When in specific use, the method can be implemented according to the following steps:
the distance between the mouth of the liquid nitrogen tank and the liquid nitrogen surface is measured by a plastic ruler.
Subtracting 1cm from the measured distance to obtain the distance for hanging the grid hanging basket 1 into the liquid nitrogen tank from the liquid nitrogen tank opening, and penetrating the inserted rod 14 into the scale hole 4 at the distance to determine the position. Through the operation, the distance between the bottom of the grid hanging basket 1 and the liquid nitrogen surface is 1cm after the grid hanging basket 1 is hung in the liquid nitrogen tank.
The ampoule 10 is filled with sperm suspension 11 and is vertically placed in the grid hanging basket 1 through the grid interlayer 2.
The height of the positioning nut 7 on the lead screw 5 is adjusted to make the positioning nut 7 higher than the top of the ampoule 10 by a distance of 0.5 cm.
The bent lug 8 on the surface of the cover plate 3 is clamped by tweezers, the central round hole of the cover plate 3 passes through the screw rod 5 and is horizontally placed on the positioning screw cap 7, and the distance between the cover plate 3 and the top of the ampoule 10 is 0.5 cm.
The grid basket 1 which has penetrated the inserted rod 14 is suspended into a liquid nitrogen tank, and the inserted rod 14 is placed at the mouth of the liquid nitrogen tank. By the operation, the sperm suspension 11 in the ampoule 10 in the grid hanging basket 1 is rapidly and uniformly cooled and frozen under the action of local cryogenic temperature generated by liquid nitrogen vapor blocked by the cover plate 3.
The above-mentioned specific implementation is the preferred embodiment of the present invention, can not be right the utility model discloses the limit, any other does not deviate from the technical scheme of the utility model and the change or other equivalent replacement modes of doing all contain within the scope of protection of the utility model.
Claims (9)
1. A sperm freezing apparatus, comprising: the device comprises a grid hanging basket, a grid interlayer, a suspension arm, an inserted bar, a cover plate and a positioning component; the davit is opened has evenly distributed's scale hole, the davit is rotationally installed in the net hanging flower basket, the inserted bar cooperatees with the scale hole of davit, the length of inserted bar is greater than the external diameter of liquid nitrogen container mouth, the net interlayer sets up in the net hanging flower basket, and the ampoule is placed in the net hanging flower basket through the net interlayer, locating component's one end is installed in the middle part of net hanging flower basket bottom, locating component's the other end passes the net interlayer and extends to the open end of net hanging flower basket, apron position is adjustably installed in locating component to be located the top of ampoule.
2. A sperm freezing apparatus as claimed in claim 1, wherein: the positioning assembly comprises a screw rod, a fixing nut and a positioning nut; the lead screw passes through fixation nut to be installed in the central authorities of net hanging flower basket bottom, the other end of lead screw extends to the open end of net hanging flower basket, positioning nut rotatably installs the other end in the lead screw, the apron is placed on positioning nut.
3. A sperm freezing apparatus as claimed in claim 2, wherein: the center of the cover plate is provided with a round hole, the upper end face of the cover plate is evenly provided with bent lugs, and the cover plate is placed on the positioning screw cap through the round hole.
4. A sperm freezing apparatus as claimed in claim 3, wherein: the number of the bent lugs is three, and the three bent lugs are distributed on the upper end face of the cover plate in an isosceles triangle shape.
5. A sperm freezing apparatus as claimed in claim 1, wherein: the suspension arm comprises two vertical rods and a cross rod; the two vertical rods are provided with scale holes which are uniformly distributed along the length direction, the interval between every two scale holes is 0.5cm, one ends of the two vertical rods are respectively and rotatably arranged on the two sides of the grid hanging basket, and the cross rod is connected with the other ends of the two vertical rods.
6. A sperm freezing apparatus as claimed in claim 5, wherein: the length of the vertical rods is 30cm, and the interval between the two vertical rods is 8 cm.
7. A sperm freezing apparatus as claimed in claim 1, wherein: the diameter of the grid hanging basket is 8cm, and the height of the grid hanging basket is 10 cm; the bottom and the periphery of the grid hanging basket are grids woven by thin copper wires, and the size of each grid is 0.7cm x 0.7 cm.
8. A sperm freezing apparatus as claimed in claim 1, wherein: the grid separation layer and the bottom of the grid hanging basket are spaced by 2cm, and the grid separation layer is formed by 1.5 cm-1.5 cm grids formed by weaving thin copper wires.
9. A sperm freezing apparatus as claimed in claim 1, wherein: the cover plate is made of metal materials, the diameter of the cover plate is 7cm, the thickness of the cover plate is smaller than 0.1cm, and the diameter of a circular hole in the center of the cover plate is 0.7 cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021348283.6U CN213404656U (en) | 2020-07-10 | 2020-07-10 | Sperm freezing device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021348283.6U CN213404656U (en) | 2020-07-10 | 2020-07-10 | Sperm freezing device |
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| CN213404656U true CN213404656U (en) | 2021-06-11 |
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| CN202021348283.6U Active CN213404656U (en) | 2020-07-10 | 2020-07-10 | Sperm freezing device |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113785823A (en) * | 2021-09-14 | 2021-12-14 | 山东省海洋科学研究院(青岛国家海洋科学研究中心) | Experimental device for accurately cooling by using liquid nitrogen tank and using method |
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2020
- 2020-07-10 CN CN202021348283.6U patent/CN213404656U/en active Active
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113785823A (en) * | 2021-09-14 | 2021-12-14 | 山东省海洋科学研究院(青岛国家海洋科学研究中心) | Experimental device for accurately cooling by using liquid nitrogen tank and using method |
| CN113785823B (en) * | 2021-09-14 | 2022-04-05 | 山东省海洋科学研究院(青岛国家海洋科学研究中心) | Experimental device for accurately cooling by using liquid nitrogen tank and using method |
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