CN213357598U - Stem cell separation and storage device - Google Patents
Stem cell separation and storage device Download PDFInfo
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- CN213357598U CN213357598U CN202021490537.8U CN202021490537U CN213357598U CN 213357598 U CN213357598 U CN 213357598U CN 202021490537 U CN202021490537 U CN 202021490537U CN 213357598 U CN213357598 U CN 213357598U
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- 238000003860 storage Methods 0.000 title claims abstract description 65
- 238000000926 separation method Methods 0.000 title claims abstract description 47
- 210000000130 stem cell Anatomy 0.000 title claims abstract description 32
- 239000003814 drug Substances 0.000 claims abstract description 55
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 50
- 238000005057 refrigeration Methods 0.000 claims abstract description 40
- 210000004027 cell Anatomy 0.000 claims abstract description 24
- 210000004369 blood Anatomy 0.000 claims abstract description 17
- 239000008280 blood Substances 0.000 claims abstract description 17
- 239000004065 semiconductor Substances 0.000 claims description 25
- 238000007789 sealing Methods 0.000 claims description 16
- 239000007788 liquid Substances 0.000 claims description 11
- 238000000151 deposition Methods 0.000 claims description 5
- 230000005611 electricity Effects 0.000 claims description 4
- 238000009434 installation Methods 0.000 claims description 2
- 210000004700 fetal blood Anatomy 0.000 description 7
- 230000000694 effects Effects 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 229940079593 drug Drugs 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 238000005119 centrifugation Methods 0.000 description 2
- 238000005336 cracking Methods 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000017525 heat dissipation Effects 0.000 description 2
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 241000282414 Homo sapiens Species 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- 238000010586 diagram Methods 0.000 description 1
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Abstract
The utility model discloses a stem cell separation and storage device, which comprises a main box body, a refrigeration unit, a peristaltic pump I and a peristaltic pump II; a main box body: the top is equipped with the separator box in it, and the bottom is equipped with the bin in it, one side swing joint of bin has the sealed door, the internally mounted of separator box has the separating centrifuge main part, the top of separating centrifuge main part is equipped with cell entry and medicament entry, the top of separator box is fixed with blood specimen and adds the funnel and deposit the medical kit, the low side export that the funnel was added to the blood specimen passes through pipeline one with the cell entry and communicates, deposit medical kit low side export and medicament entry and pass through two intercommunications of pipeline, this stem cell separation storage device, easy operation not only, consuming time is short, has improved and has prepared speed, and stem cell storage temperature is stable moreover, does not influence the data after the separation.
Description
Technical Field
The utility model relates to a stem cell separation stores technical field, specifically is a stem cell separation storage device.
Background
Stem cells are cells with unlimited or immortal self-renewal capacity and can generate at least one type of highly differentiated progeny cells, stem cell technology has become the breakthrough for human beings to overcome various difficult and complicated diseases in the present century, the stem cells are continuously judged as the first of ten scientific and technological achievements by the journal of the United states of America for two years, and data show that cord blood becomes an important source of hematopoietic stem cells, so that the separation and storage of the cord blood stem cells become particularly important;
the separation method of the umbilical cord blood hematopoietic stem cells comprises the steps of 1, placing a frozen umbilical cord blood sample into a water bath at 37 ℃ for dissolution, and then counting the cells, 2, buffering the umbilical cord blood by using a dissolution solution, placing the umbilical cord blood into an environment at 4 ℃ for centrifugation at 1500r/min after the buffer is finished, 3, cracking red blood cells by using a cracking buffer solution after the tape centrifugation is finished, and then digesting by using Dnase, 4, separating single nuclear cells by using ficoll-hypaq-ue after the digestion is finished, washing and then placing the nuclear cells into PBS/1% HSA for cell counting;
in the process of extracting stem cells, the medicament for assisting the stem cells to dissolve is required to be added usually, the conventional medicament is added manually and is measured and added, the operation of the method is complex, the time consumption is long, the preparation speed is low, the environment for storing the stem cells at present is cooled through ice blocks, the temperature of the stem cells cannot be accurately controlled during storage, and the data after the stem cells are separated are influenced.
SUMMERY OF THE UTILITY MODEL
The to-be-solved technical problem of the utility model is to overcome current defect, provide a stem cell separation storage device, easy operation not only, weak point consuming time has improved and has prepared speed, and stem cell storage temperature is stable moreover, does not influence the data after the separation, can effectively solve the problem in the background art.
In order to achieve the above object, the utility model provides a following technical scheme: a stem cell separation and storage device comprises a main box body, a refrigeration unit, a peristaltic pump I and a peristaltic pump II;
a main box body: a separation box is arranged at the top in the separation box, a storage box is arranged at the bottom in the separation box, a sealing door is movably connected to one side of the storage box, a separator main body is arranged in the separation box, a cell inlet and a medicament inlet are arranged at the top of the separator main body, a blood sample adding funnel and a medicament storage box are fixed at the top of the separation box, a low-end outlet of the blood sample adding funnel is communicated with the cell inlet through a first pipeline, a low-end outlet of the medicament storage box is communicated with the medicament inlet through a second pipeline, and a cell reservoir matched with the separator main body is arranged in the storage box;
a refrigeration unit: the refrigerator is characterized in that a refrigeration unit is mounted on the side face of the storage box and comprises a first fan, a heat radiating fin, a semiconductor refrigeration piece, a heat absorbing fin and a second fan, the semiconductor refrigeration piece is embedded on the rear side face of the storage box, the heat absorbing face of the semiconductor refrigeration piece is located inside the storage box, the heat radiating fin is fixed on the heat radiating face of the semiconductor refrigeration piece, the first fan is mounted on the side face of the heat radiating fin, the heat absorbing fin is fixed on the heat absorbing face of the semiconductor refrigeration piece, and the second fan is mounted on the side face of the heat absorbing fin;
a temperature sensor: a temperature sensor is fixed on the inner side surface of the storage box;
wherein: the top surface of the main box body is fixed with the controller, the input end of the controller is electrically connected with the output end of an external power supply, the output end of the controller is electrically connected with the input end of the first fan, the input end of the semiconductor refrigerating sheet, the input end of the second fan and the input end of the separator main body, and the output end of the temperature sensor is electrically connected with the input end of the controller.
Further, still include peristaltic pump one, the side-mounting of separator box has peristaltic pump one, peristaltic pump one sets up between the low end export of funnel and cell entry are added to the blood sample, the input of peristaltic pump one is connected with the output electricity of controller, and the effect of peristaltic pump one is mainly controlled the volume of adding with the blood sample, does not need operating personnel volume to get, convenient and fast easy operation.
The device further comprises a second peristaltic pump, the second peristaltic pump is installed on the side face of the separation box and is arranged between the outlet at the lower end of the medicine storage box and the medicine inlet, the output end of the input end controller of the second peristaltic pump is electrically connected, the quantity of the medicine added into the second peristaltic pump can be controlled, the precision is high, and the mode is simple to operate and saves time.
Further, still include the liquid level board and add the medicine lid, the side of depositing the medical kit is inlayed and is had the liquid level board, the top of depositing the medical kit is equipped with the medicine mouth, and adds the medicine lid of installation in the medicine mouth, and the liquid level board mainly is the inside dose of conveniently observing the medical kit, adds the medicine lid and conveniently adds the medicament to depositing in the medical kit.
Furthermore, the refrigerator further comprises a sealing gasket, wherein the sealing gasket is fixed on the side face of the sealing door, and the sealing gasket enables the sealing of the sealing door and the storage box to be tighter, so that the refrigerating effect of the refrigerating unit is improved.
Compared with the prior art, the beneficial effects of the utility model are that: the stem cell separation and storage device has the following advantages:
1. the pumped volume of the peristaltic pump is constant and unchangeable every time the peristaltic pump rotates for one circle, so the reproduction precision of the discharged liquid can reach five thousandths to the maximum, the cooperation of the peristaltic pump I and the peristaltic pump II is controlled by the controller, the measuring process of workers is replaced, the operation is simpler, the consumed time is short, and the preparation speed is improved;
2. according to the stem cell separation and storage device, the temperature inside the storage box can be monitored through the temperature sensor, then the temperature sensor converts a signal and transmits the signal to the controller, and then the controller controls the semiconductor refrigerating sheet to refrigerate or stop refrigerating, so that the temperature inside the storage box is constant, and data after stem cell separation is not influenced;
3. this stem cell separation storage device, easy operation not only, it is short consuming time, improved and prepared speed, stem cell storage temperature is stable moreover, does not influence the data after the separation.
Drawings
FIG. 1 is a schematic structural view of the present invention;
fig. 2 is a schematic structural diagram of the refrigeration unit of the present invention.
In the figure: the device comprises a main box body 1, a controller 2, a refrigeration unit 3, a first fan 31, a second fan 32, a semiconductor refrigeration piece 33, a heat absorption piece 34, a second fan 35, a temperature sensor 4, a liquid level plate 5, a medicine adding cover 6, a storage box 7, a separator main body 8, a separation box 9, a medicine inlet 10, a second peristaltic pump 11, a medicine storage box 12, a cell inlet 13, a blood sample adding funnel 14, a first peristaltic pump 15, a 16 sealing door and a 17 sealing gasket.
Detailed Description
The technical solutions in the embodiments of the present invention will be described clearly and completely with reference to the accompanying drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only some embodiments of the present invention, not all embodiments. Based on the embodiments in the present invention, all other embodiments obtained by a person skilled in the art without creative work belong to the protection scope of the present invention.
Referring to fig. 1-2, the present invention provides a technical solution: a stem cell separation and storage device comprises a main box body 1, a refrigeration unit 3, a first peristaltic pump 15 and a second peristaltic pump 11;
the main box body 1: the cell sampler comprises a separation box 9 arranged at the top inside the separation box, a storage box 7 arranged at the bottom inside the separation box, a sealing door 16 movably connected to one side of the storage box 7, a separator main body 8 arranged inside the separation box 9, a cell inlet 13 and a medicament inlet 10 arranged at the top of the separator main body 8, a blood sample adding funnel 14 and a medicament storage box 12 fixed at the top of the separation box 9, a first low-end outlet of the blood sample adding funnel 14 communicated with the cell inlet 13 through a first pipeline, a second low-end outlet of the medicament storage box 12 communicated with the medicament inlet 10 through a second pipeline, a cell reservoir matched with the separator main body 8 arranged inside the storage box 7, a first peristaltic pump 15 arranged at the side of the separation box 9, the first peristaltic pump 15 arranged between the low-end outlet of the blood sample adding funnel 14 and the cell inlet 13, an input end of the first peristaltic pump 15 electrically connected with an output, the automatic medicine storage box is simple in operation, convenient and fast to operate, and comprises a second peristaltic pump 11, the second peristaltic pump 11 is installed on the side face of a separation box 9, the second peristaltic pump 11 is arranged between a lower-end outlet of a medicine storage box 12 and a medicine inlet 10, the output end of an input end controller 2 of the second peristaltic pump 11 is electrically connected, the amount of added medicines can be controlled by the second peristaltic pump 11, the precision is high, the mode is simple to operate and saves time, the automatic medicine storage box further comprises a liquid level plate 5 and a medicine adding cover 6, the liquid level plate 5 is embedded in the side face of the medicine storage box 12, a medicine adding opening is formed in the top end of the medicine storage box 12, the medicine adding cover 6 is installed in the medicine adding opening, the liquid level plate 5 is mainly used for conveniently observing the amount of the medicines in the medicine storage box 12, the medicine adding cover 6 is used for conveniently adding the medicines into the medicine storage box 12, a sealing gasket 17 is further included, the, the refrigeration effect of the refrigeration unit 3 is improved;
the refrigeration unit 3: the refrigeration unit 3 is mounted on the side face of the storage box 7, the refrigeration unit 3 comprises a first fan 31, a heat radiating fin 32, a semiconductor refrigeration fin 33, a heat absorbing fin 34 and a second fan 35, the semiconductor refrigeration fin 33 is embedded on the rear side face of the storage box 7, the heat absorbing face of the semiconductor refrigeration fin 33 is located inside the storage box 7, the heat radiating fin 32 is fixed on the heat radiating face of the semiconductor refrigeration fin 33, the first fan 31 is mounted on the side face of the heat radiating fin 32, the heat absorbing fin 34 is fixed on the heat absorbing face of the semiconductor refrigeration fin 33, and the second fan 35 is mounted on the side face of the heat;
temperature sensor 4: a temperature sensor 4 is fixed on the inner side surface of the storage box 7;
wherein: the separator further comprises a controller 2, the controller 2 is fixed on the top surface of the main box body 1, the input end of the controller 2 is electrically connected with the output end of an external power supply, the output end of the controller 2 is electrically connected with the input end of the first fan 31, the input end of the semiconductor refrigerating sheet 33, the input end of the second fan 35 and the input end of the separator main body 8, and the output end of the temperature sensor 4 is electrically connected with the input end of the controller 2.
When in use: firstly, an umbilical cord blood sample from which stem cells need to be extracted is placed into a blood sample adding funnel 14, a medicine adding cover 6 is opened, a medicine to be used is added, then a controller 2 controls a peristaltic pump I15 to work, so that the blood sample added into the blood sample adding funnel 14 enters a separator main body 8, meanwhile, the controller 2 controls a peristaltic pump II 11 to work, so that the corresponding medicine is added into the separator main body 8, the pumped volume of the peristaltic pump is constant and invariable every circle of rotation of the peristaltic pump, the reproduction precision of the discharged liquid can reach five thousandths, and can reach one percent in the normal condition, so that the constant peristaltic pump is also called as a constant flow pump, the precision of the adding amount of the medicine and the blood sample can be improved by using the peristaltic pump, the preparation effect is improved, then the controller 2 controls the separator main body 8 to work for separation, the separated cells are stored in a cell storage device in a storage tank 7, at this moment, control semiconductor refrigeration piece 33 through controller 2 and refrigerate, accelerate the inside gas exchange speed of bin 7 through fan two 35, shorten the refrigeration time, accelerate the radiating rate of fin 32 through fan one 31, make the faster heat dissipation of semiconductor refrigeration piece 33, temperature sensor 4 can monitor the inside temperature of bin 7, then send the signal transformation to controller 2, then controller 2 again controls semiconductor refrigeration piece 33 refrigeration or stops refrigerating, make the inside temperature of bin 7 invariable, do not influence the data after the stem cell separation.
It is worth noting that, in the embodiment, the controller 2 is preferably an STM32 single chip microcomputer, the first fan 31 and the second fan 35 are YRD heat dissipation fans, the semiconductor refrigeration sheet 33 can be customized according to actual sizes in jinzhi electromechanical technologies limited, shenzhen, the temperature sensor 4 can be a DS18B20 temperature sensor, the peristaltic pump two 11 and the peristaltic pump one 15 can be KCP micro peristaltic pumps, and the controller 2 controls the first fan 31, the second fan 35, the semiconductor refrigeration sheet 33, the peristaltic pump two 11 and the peristaltic pump one 15 to work by a method commonly used in the prior art.
Although embodiments of the present invention have been shown and described, it will be appreciated by those skilled in the art that changes, modifications, substitutions and alterations can be made in these embodiments without departing from the principles and spirit of the invention, the scope of which is defined in the appended claims and their equivalents.
Claims (5)
1. A stem cell separation and storage device is characterized in that: comprises a main box body (1), a refrigeration unit (3), a peristaltic pump I (15) and a peristaltic pump II (11);
main case (1): a separation box (9) is arranged at the top of the separation box, a storage box (7) is arranged at the inner bottom of the separation box, a sealing door (16) is movably connected to one side of the storage box (7), a separator main body (8) is arranged inside the separation box (9), a cell inlet (13) and a medicament inlet (10) are arranged at the top of the separator main body (8), a blood sample adding funnel (14) and a medicament storage box (12) are fixed at the top of the separation box (9), a low-end outlet of the blood sample adding funnel (14) is communicated with the cell inlet (13) through a first pipeline, a low-end outlet of the medicament storage box (12) is communicated with the medicament inlet (10) through a second pipeline, and a cell storage device matched with the separator main body (8) is arranged inside the;
refrigeration unit (3): a refrigeration unit (3) is installed on the side face of the storage box (7), the refrigeration unit (3) comprises a first fan (31), a heat radiating fin (32), a semiconductor refrigeration fin (33), a heat absorbing fin (34) and a second fan (35), the semiconductor refrigeration fin (33) is embedded on the rear side face of the storage box (7), the heat absorbing face of the semiconductor refrigeration fin (33) is located inside the storage box (7), the heat radiating fin (32) is fixed on the heat radiating face of the semiconductor refrigeration fin (33), the first fan (31) is installed on the side face of the heat radiating fin (32), the heat absorbing face of the semiconductor refrigeration fin (33) is fixed with the heat absorbing fin (34), and the second fan (35) is installed on the side face of the heat absorbing fin (34);
temperature sensor (4): a temperature sensor (4) is fixed on the inner side surface of the storage box (7);
wherein: still include controller (2), the top surface of main tank body (1) is fixed with controller (2), the input and the output electricity of external power source of controller (2) are connected, the output of controller (2) is connected with the input of fan one (31), the input of semiconductor refrigeration piece (33), the input of fan two (35) and the input of separating centrifuge main part (8) electricity, the output of temperature sensor (4) is connected with the input electricity of controller (2).
2. The stem cell separation and storage device of claim 1, wherein: the cell separation device is characterized by further comprising a first peristaltic pump (15), wherein the first peristaltic pump (15) is installed on the side face of the separation box (9), the first peristaltic pump (15) is arranged between the lower-end outlet of the blood sample adding funnel (14) and the cell inlet (13), and the input end of the first peristaltic pump (15) is electrically connected with the output end of the controller (2).
3. The stem cell separation and storage device of claim 1, wherein: the medicine storage box is characterized by further comprising a second peristaltic pump (11), the second peristaltic pump (11) is installed on the side face of the separation box (9), the second peristaltic pump (11) is arranged between the outlet at the lower end of the medicine storage box (12) and the medicine inlet (10), and the output end of the input end controller (2) of the second peristaltic pump (11) is electrically connected.
4. The stem cell separation and storage device of claim 1, wherein: still include liquid level board (5) and add medicine lid (6), the side of depositing medical kit (12) is inlayed and is had liquid level board (5), the top of depositing medical kit (12) is equipped with the medicine mouth, and adds medicine lid (6) of installation in the medicine mouth.
5. The stem cell separation and storage device of claim 1, wherein: the sealing door further comprises a sealing gasket (17), and the sealing gasket (17) is fixed on the side surface of the sealing door (16).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202021490537.8U CN213357598U (en) | 2020-07-26 | 2020-07-26 | Stem cell separation and storage device |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202021490537.8U CN213357598U (en) | 2020-07-26 | 2020-07-26 | Stem cell separation and storage device |
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| CN213357598U true CN213357598U (en) | 2021-06-04 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114532331A (en) * | 2022-03-04 | 2022-05-27 | 武汉贝索细胞科学技术研究院有限公司 | Long-time cryopreservation device for stem cell samples for medical experiments |
-
2020
- 2020-07-26 CN CN202021490537.8U patent/CN213357598U/en active Active
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN114532331A (en) * | 2022-03-04 | 2022-05-27 | 武汉贝索细胞科学技术研究院有限公司 | Long-time cryopreservation device for stem cell samples for medical experiments |
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Effective date of registration: 20210809 Address after: 400000 3-1, building 6, No. 6, Gangcheng East Ring Road, Jiangbei District, Chongqing Patentee after: Yuanchuanggene (Chongqing) Cell Application Technology Research Institute Co., Ltd Patentee after: ZHENGZHOU REVO GENE INDUSTRY Co.,Ltd. Patentee after: Chongqing Gongxin Business Management Co.,Ltd. Address before: 230000 Lijing garden, 277 Qianshan Road, Shushan District, Hefei City, Anhui Province Patentee before: Wang Yuhu |