CN213357587U

CN213357587U - Branching bacillus detects with reinforcing and detects cultivation box

Info

Publication number: CN213357587U
Application number: CN202021749859.XU
Authority: CN
Inventors: 刘鸿雁
Original assignee: Guangzhou Dry Kang Medical Technology Co ltd
Current assignee: Guangzhou Dry Kang Medical Technology Co ltd
Priority date: 2020-08-20
Filing date: 2020-08-20
Publication date: 2021-06-04
Anticipated expiration: 2030-08-20

Abstract

The utility model provides a mycobacteria detects and cultivates box with reinforcing detection belongs to the mycobacteria and cultivates and detect technical field for solve the problem that mycobacteria detection operation is loaded down with trivial details and detection effect is poor. The box comprises a box body and a box cover, wherein a plurality of hinges are arranged between one side of the box body and one side of the box cover, two symmetrical hasp locks are arranged between the other side of the box body and the other side of the box cover, a culture medium box and a medicament test box are hinged in the box body, a containing groove is formed in the box body, a placing box is arranged in the containing groove in a sliding mode, a plurality of sterile suction nozzles are arranged in the placing box, and a locking buckle is arranged between the placing box and the box body; the utility model has a sealed constant temperature space, ensures the stable growth of strains, is convenient for detection and contrast, can provide simple and clear medicine sensitivity judgment results, can obtain the bacterium type result, greatly shortens the detection time, and only needs to judge the result by naked eyes; the culture medium box and the medicament test box are convenient to take out and store, and the operation is simple and the use is convenient.

Description

Branching bacillus detects with reinforcing and detects cultivation box

Technical Field

The utility model belongs to the technical field of the detection is cultivateed to the mycobacterium, a cultivate the box, especially a mycobacteria detects and cultivates box with reinforcing detection.

Background

The detection of mycobacterial species in clinical species is important as a clinical diagnostic tool and many clinical methods for detecting and identifying mycobacterial species in a sample require analysis of the physical properties of the bacteria (e.g. acid-fast staining and microscopic detection of the bacilli), physiological properties (e.g. growth on defined media) or biochemical properties (e.g. membrane lipid composition). These methods require relatively high concentrations of bacteria in the sample to be detected, can be subjective depending on the experience and expertise of the clinical technician, and are time consuming. Because mycobacterial species are often difficult to grow in vitro and may take several weeks to reach useful culture densities, these methods may also result in the expense associated with quarantining infected individuals before delayed patient treatment and diagnosis is complete.

However, because of the slow growth rate and the need for an enrichment medium for growth of mycobacteria in culture, laboratory diagnosis of mycobacteria is conventionally based on acid-fast bacterial staining and culture of organisms followed by biochemical assays. Due to the slow growth and long generation time of mycobacteria, accurate laboratory diagnosis of mycobacteria by conventional techniques may take up to 6 weeks, be complicated in structure, difficult to handle, and not practical.

Therefore, the reinforced detection culture box for detecting the mycobacterium is safe and effective, convenient to operate, short in test time and wide in test medicine range.

SUMMERY OF THE UTILITY MODEL

The utility model aims at having the above-mentioned problem to current technique, provided a branching fungus detects with reinforcing and detects cultivation box, the technical problem that the device will solve is: how to rapidly culture and effectively detect the mycobacteria.

The purpose of the utility model can be realized by the following technical proposal:

the utility model provides a branching bacillus detects and detects cultivation box with reinforcing, includes box body and lid, be provided with a plurality of hinges between one side of box body and one side of lid, be provided with the hasp of two symmetries between the opposite side of box body and the opposite side of lid, inside articulated culture medium box and the medicament test box of having of box body, culture medium box and medicament test box symmetry set up, and the inside of box body is equipped with accomodates the groove, accomodates the inslot portion and slides and be provided with and place the box, and the inside of placing the box is equipped with a plurality of aseptic suction nozzles, places and is provided with the closure between box and the box body and.

The utility model discloses a theory of operation: opening the box cover, taking out the culture medium box and the medicament test box, placing the box and also placing some using tools, wherein a plurality of solid culture mediums and liquid culture mediums are arranged in the culture medium box, a sterile diluent, a mixed bacteria inhibitor and a nutrient additive are arranged in the medicament test box, opening the placing box, taking out the sterile suction nozzle, respectively dripping the sterile diluent, the mixed bacteria inhibitor and the nutrient additive on the solid culture mediums and the liquid culture mediums by using the sterile suction nozzle, enhancing the detection contrast effect, then planting the mycobacteria on the solid culture mediums and the liquid culture mediums, then collecting the culture medium box and the reagent test box, covering the box cover, carrying out constant temperature culture, observing the result after 7-10 days, and determining that the white thallus precipitate appearing at the bottom of the hole is positive, it indicates that bacteria grow in the hole, and the drug sensitivity is judged according to the growth result of each culture medium, thereby effectively enhancing the detection effect.

The side of the box body is fixed with a handle and a locking shaft, the locking shaft is positioned below the handle, the bottom inside the box body is fixed with a heat preservation heating plate, the heat preservation heating plate is positioned below the culture medium box and the reagent testing box, and four hinged connecting rods are respectively and symmetrically arranged on two sides inside the box body in a rotating mode.

Structure more than adopting, this device of taking is put on limit to the handle, and the closure axle is used for the closure to place the box, and the constant temperature is guaranteed to the heat preservation hot plate, provides the constant temperature culture condition, and convenient access culture medium box of articulated connecting rod and medicament test box.

The hasp comprises a hasp pressing buckle and a hasp body, the hasp pressing buckle is fixed on the side face of the box body, the hasp body is fixed on the side face of the box cover, and the hasp pressing buckle can be detachably clamped on the corresponding hasp body.

By adopting the structure, the hasp pressing buckle can be detachably clamped on the corresponding hasp, the device can be conveniently and effectively locked and sealed, the hasp pressing buckle can be locked, and the locking is safer.

The improved multifunctional box cover is characterized in that a circle of sealing ring strip is fixed on the bottom surface of the box cover, an observation window is arranged on the box cover, a temperature regulating switch, temperature scale marks and a temperature sensing display screen are arranged on the box cover, a storage battery is arranged inside the box cover, the storage battery is respectively electrically connected with the temperature regulating switch and the temperature sensing display screen, and the temperature regulating switch is electrically connected with the heat preservation heating plate.

Structure more than adopting, the lid is fashionable, and the extrusion of sealing coil strip is guaranteed sealedly between box body and lid, and the measurement personnel can observe the macroscopic change of mycobacteria kind through the observation window, rotates temperature regulation switch, and the directional temperature scale mark that corresponds of temperature regulation switch adjusts the battery to the power supply and the switch of heat preservation hot plate to adjust the heating heat preservation of heat preservation hot plate, temperature-sensitive display screen real-time supervision and show the inside temperature of this device.

The culture medium box is articulated with four articulated connecting rods on one side, a plurality of culture medium grooves are formed in the culture medium box, the medicament test box is articulated with the four articulated connecting rods on the other side, a plurality of medicament bins are arranged in the medicament test box, bin covers are clamped on the medicament bins, a medicament sensitive test board is arranged on the medicament test box, and a negative control hole, a positive control hole and a plurality of medicament containing holes which are arranged in a matrix manner and coated with different anti-tuberculosis medicaments are arranged on the medicament sensitive test board.

Structure more than adopting, inside a plurality of solid medium and the liquid medium of being equipped with respectively of a plurality of culture medium grooves, the inside aseptic diluent that is equipped with respectively of medicament storehouse, miscellaneous fungus inhibitor and nutritional supplement, the cang gai is on the medicament storehouse, avoid unrestrained, be provided with negative contrast hole on the quick test panel of medicine, positive contrast hole and a plurality of are matrix arrangement and the peridium has the hole that contains of different anti-tuberculosis medicine, the convenient control that detects fast, both can provide simple and clear quick judgement result of medicine, again can obtain the bacterial type result, check-out time shortens greatly, and only need the naked eye interpretation result.

The side of placing the box is fixed with the installation axle, and the one end of locking buckle rotates and sets up at installation epaxial, and the other end of locking buckle can be dismantled and shut ground joint and epaxial at the locking, places the inside placing house and the storage compartment that are equipped with of box, and the storage compartment is inside to be fixed with a plurality of storage card slot boards that are fixed with, and aseptic suction nozzle joint is in storage card slot board top.

Structure more than adopting, the other end of closure buckle can be dismantled and shut ground joint on the closure is epaxial, and convenient the unscrewing fast pulls out and places the box, places some instruments of conveniently placing in the storehouse inside, and aseptic suction nozzle joint is in accomodating draw-in groove board top, conveniently takes.

Compared with the prior art, the enhanced detection culture box for detecting the mycobacterium has the advantages that:

a sealed constant-temperature space is formed by matching the box body, the box cover and the heat-preservation heating plate, so that stable growth is ensured; the culture medium groove is internally provided with a plurality of solid culture mediums and liquid culture mediums respectively, the medicament bin is internally provided with a sterile diluent, a mixed bacteria inhibitor and a nutrient additive respectively, the medicament bin is covered on the medicament bin to avoid spilling, the medicament sensitive test board is provided with a negative control hole, a positive control hole and a plurality of medicament-containing holes which are arranged in a matrix manner and coated with different antituberculosis medicaments, the detection and control are conveniently and quickly carried out, not only can a simple and clear medicament sensitive judgment result be provided, but also a bacterial type result can be obtained, the detection time is greatly shortened, and the result only needs to be judged and read by naked eyes; the culture medium box and the reagent test box are respectively matched with the hinged connecting rod, so that the culture medium box and the reagent test box are convenient to take out and store, and the operation is simple and convenient; the temperature setting switch, the temperature scale lines and the temperature sensing display screen are adopted, so that the set temperature can be conveniently adjusted and observed, and the use is convenient.

Drawings

Fig. 1 is a schematic perspective view of the present invention when opened;

fig. 2 is a schematic perspective view of the present invention when closed;

in the figure: 1-box body, 1 a-containing groove, 1 b-locking shaft, 2-box cover, 2 a-sealing ring strip, 2 b-observation window, 2 c-temperature regulating switch, 2 d-temperature scale mark, 2 e-temperature sensitive display screen, 3-hinge, 4-culture medium box, 4 a-culture medium groove, 5-hinged connecting rod and 6-heat preservation heating plate, 7-handle, 8-placing box, 8 a-mounting shaft, 8 b-placing bin, 8 c-containing bin, 8 d-containing clamping groove plate, 9-locking buckle, 10-sterile suction nozzle, 11-medicament testing box, 11 a-medicament bin, 11 b-medicament sensitivity testing plate, 12-locking buckle, 12 a-locking press buckle and 12 b-buckle.

Detailed Description

The technical solution of the present patent will be described in further detail with reference to the following embodiments.

Reference will now be made in detail to embodiments of the present patent, examples of which are illustrated in the accompanying drawings, wherein like or similar reference numerals refer to the same or similar elements or elements having the same or similar function throughout. The embodiments described below with reference to the drawings are exemplary only for the purpose of explaining the present patent and are not to be construed as limiting the present patent.

In the description of this patent, it is to be understood that the terms "center," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like are used in the orientations and positional relationships indicated in the drawings for the convenience of describing the patent and for the simplicity of description, and are not intended to indicate or imply that the referenced devices or elements must have a particular orientation, be constructed and operated in a particular orientation, and are not to be considered limiting of the patent.

In the description of this patent, it is noted that unless otherwise specifically stated or limited, the terms "mounted," "connected," and "disposed" are to be construed broadly and can include, for example, fixedly connected, disposed, detachably connected, disposed, or integrally connected and disposed. The specific meaning of the above terms in this patent may be understood by those of ordinary skill in the art as appropriate.

Referring to fig. 1-2, the embodiment provides a reinforced detection culture box for detecting mycobacteria, which includes a box body 1 and a box cover 2, wherein a plurality of hinges 3 are arranged between one side of the box body 1 and one side of the box cover 2, two symmetrical hasps 12 are arranged between the other side of the box body 1 and the other side of the box cover 2, a culture medium box 4 and a medicament test box 11 are hinged inside the box body 1, the culture medium box 4 and the medicament test box 11 are symmetrically arranged, a containing groove 1a is arranged inside the box body 1, a placing box 8 is arranged inside the containing groove 1a in a sliding manner, a plurality of sterile suction nozzles 10 are arranged inside the placing box 8, and a locking buckle 9 is arranged between the placing box 8 and the box body 1; opening the box cover 2, taking out the culture medium box 4 and the medicament test box 11, the placing box 8 can also be placed with some using tools, the culture medium box 4 is internally provided with a plurality of solid culture mediums and liquid culture mediums, the medicament test box 11 is internally provided with sterile diluent, a mixed bacteria inhibitor and a nutrient additive, the placing box 8 is opened, the sterile suction nozzle 10 is taken out, the sterile diluent, the mixed bacteria inhibitor and the nutrient additive are respectively dripped on the solid culture mediums and the liquid culture mediums by the sterile suction nozzle 10 to enhance the detection contrast effect, then mycobacteria are planted on the solid culture mediums and the liquid culture mediums, then the culture medium box 4 and the medicament test box 11 are collected, the box cover 2 is closed for constant temperature culture, the observation result after 7-10 days, the result shows that the white thallus precipitation at the bottom of the hole is positive, the bacteria grow in the hole, the medicament sensitivity is judged according to the growth result of each culture medium, the detection effect is effectively enhanced.

A handle 7 and a locking shaft 1b are fixed on the side surface of the box body 1, the locking shaft 1b is positioned below the handle 7, and a heat-preservation heating plate 6 is fixed at the bottom inside the box body 1, in the embodiment, the fixing modes are all connected by bolts, the heat-preservation heating plate 6 is a product in the prior art and can be directly purchased in the market to be used in a proper size, the heat-preservation heating plate 6 is positioned below the culture medium box 4 and the medicament testing box 11, and four hinged connecting rods 5 are symmetrically and rotatably arranged on two sides inside the box body 1 respectively; this device of taking is put limit to handle 7, and locking axle 1b is used for the closure to place box 8, and the constancy of temperature is guaranteed to heat preservation hot plate 6, for culture medium box 4 and medicament test box 11 provide the constant temperature culture condition, and articulated connecting rod 5 conveniently accesses culture medium box 4 and medicament test box 11.

The hasp 12 comprises a hasp pressing buckle 12a and a hasp 12b, the hasp pressing buckle 12a is fixed on the side surface of the box body 1, and the hasp 12b is fixed on the side surface of the box cover 2.

A circle of sealing ring strips 2a are fixed on the bottom surface of the box cover 2, in the embodiment, the fixing mode is bonding, an observation window 2b is arranged on the box cover 2, a temperature regulating switch 2c, a temperature scale mark 2d and a temperature sensing display screen 2e are arranged on the box cover 2, a storage battery is arranged in the box cover 2, the storage battery is respectively electrically connected with the temperature regulating switch 2c and the temperature sensing display screen 2e, and the temperature regulating switch 2c is electrically connected with the heat preservation heating plate 6; when lid 2 was closed, sealing strip 2a extruded between box body 1 and lid 2, guarantee sealedly, the measurement personnel can be through observation window 2b, observe the macroscopic change of mycobacterial kind, rotate temperature regulation switch 2c, temperature regulation switch 2c points to corresponding temperature scale mark 2d, adjust the battery to the power supply and the switch of heat preservation hot plate 6, thereby adjust heating heat preservation, the inside temperature of this device is monitored and shown in temperature-sensing display screen 2e real-time, each above-mentioned electrical components is prior art product in this embodiment, can directly purchase the use in the market.

The culture medium box 4 is hinged with four hinged connecting rods 5 on one side, a plurality of culture medium grooves 4a are formed in the culture medium box 4, the medicament test box 11 is hinged with the four hinged connecting rods 5 on the other side, a plurality of medicament bins 11a are arranged in the medicament test box 11, bin covers are clamped on the medicament bins 11a, a medicament sensitive test board 11b is arranged on the medicament test box 11, and a negative control hole, a positive control hole and a plurality of medicament-containing holes which are arranged in a matrix manner and coated with different antituberculosis medicaments are formed in the medicament sensitive test board 11 b; a plurality of solid culture mediums and liquid culture mediums are respectively arranged inside a plurality of culture medium grooves 4a, aseptic diluent, mixed bacteria inhibitor and nutritional additive are respectively arranged inside the medicament storehouse 11a, a storehouse cover is covered on the medicament storehouse 11a, the sprinkling is avoided, a negative control hole is arranged on the medicament sensitive test plate 11b, the positive control hole and a plurality of medicament containing holes which are arranged in a matrix and coated with different antituberculosis medicaments are arranged, the detection control is conveniently and quickly carried out, a simple and clear medicament sensitive judgment result can be provided, a bacterial type result can be obtained, the detection time is greatly shortened, and the result can be judged only by naked eyes.

Place the side of box 8 and be fixed with installation axle 8a, in this embodiment, bolted connection is adopted to above-mentioned fixed mode, the one end rotation setting of locking buckle 9 is on installation axle 8a, the other end joint that can open and shut of locking buckle 9 is on locking axle 1b, convenient quick unscrewing, draw out and place box 8, place box 8 inside and be equipped with and place storehouse 8b and storage compartment 8c, storage compartment 8c is inside to be fixed with a plurality of accomodating draw-in groove boards 8d that are fixed with, in this embodiment, above-mentioned fixed mode all adopts integrated into one piece, aseptic suction nozzle 10 joint is in accomodating draw-in groove board 8d top and is placed the inside some instruments of conveniently placing in storehouse 8b, aseptic suction nozzle 10 joint is in accomodating draw-in groove board 8d top, conveniently take.

The utility model discloses a theory of operation: opening a hasp 12a, opening a box cover 2 around a hinge 3, pulling a culture medium box 4 and a medicament test box 11 outwards to rotate around a hinged connecting rod 5, taking out the culture medium box 4 and the medicament test box 11, detachably clamping the other end of a locking buckle 9 on a locking shaft 1b, conveniently and quickly unscrewing, respectively filling a plurality of solid culture mediums and liquid culture mediums in an internal culture medium groove 4a of the culture medium box 4, respectively filling sterile diluent, a foreign bacteria inhibitor and a nutrient additive in a medicament bin 11a of the medicament test box 11, respectively filling sterile diluent, a foreign bacteria inhibitor and a nutrient additive in the sterile diluent, the foreign bacteria inhibitor and the nutrient additive, pulling out a placing box 8, taking out a sterile suction nozzle 10, respectively dripping the sterile diluent, the foreign bacteria inhibitor and the nutrient additive on the solid culture mediums and the liquid culture mediums by using the sterile suction nozzle 10, enhancing the detection contrast effect, then planting mycobacteria on the solid culture mediums and the liquid culture mediums, then the culture medium box 4 and the medicament test box 11 are folded, the box cover 2 is covered, the temperature regulating switch 2c is rotated, the temperature regulating switch 2c points to the corresponding temperature scale mark 2d, the power supply and the switch of the storage battery to the heat preservation heating plate 6 are regulated, the heating and heat preservation of the heat preservation heating plate 6 are regulated, the temperature inside the device is monitored and displayed in real time by the temperature-sensitive display screen 2e, the constant temperature culture is carried out, after 7-10 days, a tester can observe the macroscopic change of mycobacteria species through the observation window 2b, the white thallus precipitation appearing at the bottom of the hole is positive, the growth of bacteria in the hole is shown, the medicament sensitivity is judged according to the growth result of each culture medium, the detection effect is effectively enhanced, the medicament-sensitive test board 11b is provided with a negative control hole, a positive control hole and a plurality of medicament-containing holes which are arranged in a matrix manner, the detection contrast is conveniently and rapidly carried out, not only can a simple and clear drug sensitivity judgment result be provided, but also a bacterial type result can be obtained, the detection time is greatly shortened, and the result is only needed to be judged and read by naked eyes.

In conclusion, a sealed constant-temperature space is formed by matching the box body 1, the box cover 2 and the heat-preservation heating plate 6, so that stable growth is ensured; the culture medium groove 4a is internally provided with a plurality of solid culture mediums and liquid culture mediums respectively, the medicament bin 11a is internally provided with sterile diluent, a mixed bacteria inhibitor and a nutrient additive respectively, the bin cover is covered on the medicament bin 11a to avoid spilling, the medicament sensitive test board 11b is provided with a negative control hole, a positive control hole and a plurality of medicament-containing holes which are arranged in a matrix manner and coated with different antituberculosis medicaments, the detection and control are convenient and rapid, not only can a simple and clear medicament sensitive judgment result be provided, but also a bacterial type result can be obtained, the detection time is greatly shortened, and the result can be judged only by naked eyes; the culture medium box 4 and the reagent test box 11 are respectively matched with the hinged connecting rod 5, so that the taking out and the storage are convenient, and the operation is simple and convenient; the temperature adjusting switch 2c, the temperature scale lines 2d and the temperature sensing display screen 2e are adopted to conveniently adjust and observe the set temperature, and the use is convenient.

Although the preferred embodiments of the present patent have been described in detail, the present patent is not limited to the above embodiments, and various changes can be made without departing from the spirit of the present patent within the knowledge of those skilled in the art.

Claims

1. The utility model provides a branching bacillus detects and detects cultivation box with reinforcing, including box body (1) and lid (2), be provided with a plurality of hinges (3) between one side of box body (1) and one side of lid (2), a serial communication port, be provided with hasp (12) of two symmetries between the opposite side of box body (1) and the opposite side of lid (2), box body (1) inside articulates there are culture medium box (4) and medicament test box (11), culture medium box (4) and medicament test box (11) symmetry set up, the inside of box body (1) is equipped with accomodates groove (1a), it is provided with inside slip in groove (1a) and places box (8), the inside of placing box (8) is equipped with a plurality of aseptic suction nozzles (10), it is provided with locking buckle (9) to place between box (8) and box body (1).

2. The enhanced detection culture box for detecting mycobacterium according to claim 1, wherein a handle (7) and a locking shaft (1b) are fixed on the side surface of the box body (1), the locking shaft (1b) is positioned below the handle (7), a heat preservation heating plate (6) is fixed at the bottom inside the box body (1), the heat preservation heating plate (6) is positioned below the culture medium box (4) and the medicament test box (11), and four hinged connecting rods (5) are symmetrically and rotatably arranged on two sides inside the box body (1) respectively.

3. The culture box for enhanced detection of mycobacteria detection according to claim 1, wherein the latch (12) comprises a latch press button (12a) and a latch (12b), the latch press button (12a) is fixed on the side of the box body (1), the latch (12b) is fixed on the side of the box cover (2), and the latch press button (12a) is detachably engaged with the corresponding latch (12 b).

4. The reinforced detection culture box for detecting mycobacterium according to claim 2, wherein a circle of sealing ring strip (2a) is fixed on the bottom surface of the box cover (2), the box cover (2) is provided with an observation window (2b), a temperature regulating switch (2c), a temperature scale mark (2d) and a temperature sensing display screen (2e) are arranged on the box cover (2), a storage battery is arranged inside the box cover (2), the storage battery is respectively electrically connected with the temperature regulating switch (2c) and the temperature sensing display screen (2e), and the temperature regulating switch (2c) is electrically connected with the heat preservation heating plate (6).

5. The enhanced detection culture box for detecting the mycobacterium as claimed in claim 2 or 4, wherein the culture medium box (4) is hinged to four hinged connecting rods (5) on one side, a plurality of culture medium grooves (4a) are formed in the culture medium box (4), the reagent test box (11) is hinged to four hinged connecting rods (5) on the other side, a plurality of reagent bins (11a) are arranged in the reagent test box (11), bin covers are clamped on the reagent bins (11a), a reagent sensitivity test board (11b) is arranged on the reagent test box (11), and negative control holes, positive control holes and a plurality of reagent containing holes which are arranged in a matrix and are coated with different anti-tuberculosis drugs are arranged on the reagent sensitivity test board (11 b).

6. The culture box for enhanced detection of mycobacteria detection according to claim 5, wherein the side surface of the placing box (8) is fixed with an installation shaft (8a), one end of a locking buckle (9) is rotatably arranged on the installation shaft (8a), the other end of the locking buckle (9) is detachably clamped on the locking shaft (1b), a placing cabin (8b) and a storage cabin (8c) are arranged in the placing box (8), a plurality of storage slot plates (8d) are fixed in the storage cabin (8c), and the sterile suction nozzle (10) is clamped above the storage slot plates (8 d).