CN213284502U - Cut-flower chrysanthemum tissue culture sterilization device - Google Patents

Abstract

The utility model discloses a cut-flower chrysanthemum tissue culture sterilization device, including installation shell, high-pressure lid, the high-pressure pot body, steam advances pipe, steam outlet, vacuum pump, tissue culture bottleholder and footing, the device still includes water pump, water tank and high-pressure atomizer, high-pressure atomizer seal installation is on high-pressure lid, high-pressure atomizer connects the water pump, water pump and vacuum pump all connect the water tank, all link to each other through the connecting pipe between water pump and the high-pressure atomizer and between steam outlet and the vacuum pump, steam advances pipe, steam outlet and high-pressure atomizer and all installs the check valve; through setting up the water tank, make in the refrigerated steam can flow to the water tank, after the sterilization is accomplished, spray into the pressure cooker internal with the aseptic water of cooling of water tank through water pump and high pressure atomizer, the internal high pressure of pressure cooker and high temperature make the aseptic water of cooling vaporize rapidly this moment, through the reentry flow water tank again, at the backward flow in-process, carry out the purpose in order to reach rapid cooling in the lump with the internal heat of pressure cooker.

Description

Cut-flower chrysanthemum tissue culture sterilization device

Technical Field

The utility model relates to a sterilizing installation technical field, concretely relates to cut flower chrysanthemum group banks up sterilization apparatus with earth.

Background

The cut chrysanthemum is one of the four cut flowers in the world, has the first yield of the four cut flowers, has the advantages of various flower types, rich colors, wide application, transport and storage resistance, long service life of bottle inserts, easy propagation and cultivation, annual supply, low cost, high yield and the like, and is mostly applied to the cut chrysanthemum seedling cultivation process by adopting a tissue culture mode in the prior art. Plant tissue culture, also called isolated culture, refers to a technique of separating desired tissues, organs or cells, protoplasts, etc. from a plant body, and culturing under an artificial control condition by aseptic manipulation to obtain regenerated whole plants or produce other products having economic value. Therefore, sterilization of the culture medium is a crucial part of the tissue culture process.

The sterilization of the culture medium refers to that all microorganisms, including pathogenic and nonpathogenic microorganisms and spores, in the culture medium are killed by a physical or chemical method so as to achieve the sterility guarantee level. In the prior art, the culture medium is generally sterilized by high-pressure steam. The pre-vacuum pressure steam sterilizing pot is the latest sterilizing equipment developed in the prior art, and is provided with a vacuum pump, wherein the inside of the pre-vacuum pressure steam sterilizing pot is vacuumized before steam is introduced to form negative pressure so as to facilitate the penetration of the steam. The vacuum high-pressure steam sterilization method can sterilize at the temperature of 132-134 ℃ for 4min under the pressure of 205.8kPa, and the sterilization effect is excellent. The existing vacuum high-pressure steam sterilization method can meet the daily production research requirements of people, but still has the problems that the sterilization needs to be stood for a long time to naturally cool the sterilization medium, and drying equipment needs to be additionally arranged to dry the sterilization medium.

SUMMERY OF THE UTILITY MODEL

Not enough to prior art, the utility model provides a cut flower chrysanthemum group banks up sterilization apparatus with earth, this design possesses and to make sterilization apparatus rapid cooling to can carry out dry advantage to the disinfection culture medium, solved and to have stood for a long time after the sterilization and make its natural cooling, and need additionally install drying equipment and carry out dry problem to the disinfection culture medium.

The utility model discloses a cut-flower chrysanthemum tissue culture sterilization device, including the installation shell, the high-pressure lid, the hyperbaric pot body, steam advances the pipe, steam outlet, the vacuum pump, group banks up bottleholder and footing, the high-pressure lid, the hyperbaric pot body, vacuum pump and aspiration pump are all installed in the installation shell, the high-pressure lid agrees with the connection and seals through fastening bolt in agreeing with the junction with the hyperbaric pot body, hyperbaric pot body bottom center opening and steam advance the pipe, the steam outlet has been seted up to the lateral wall of the hyperbaric pot body, steam outlet connects the vacuum pump, group banks up the bottleholder and places in the hyperbaric pot body, the footing is installed in the installation shell bottom, the device still includes the water pump, water tank and high-pressure atomizer, high-pressure atomizer seal installation is on the high-pressure lid, high-pressure atomizer connects the water pump, water pump and vacuum pump all connect, the steam inlet pipe, the steam outlet and the high-pressure atomizing nozzle are all provided with one-way valves.

The utility model discloses a cut-flower chrysanthemum tissue culture sterilization device, wherein the autoclave body still includes the inner pot body, sterilization zone and circulation district, the inner pot body is the cup and has seted up the opening in the bottom center, inner pot body top and bottom opening all have the flange that extends outward, the inner pot body passes through the flange and welds in autoclave inner wall, the inside sterilization zone that is of inner pot body, be circulation district between inner pot body outer wall and autoclave inner wall, steam outlet sets up in the circulation district, inner pot body top flange is kept away from steam outlet position and has been seted up through the opening and make sterilization zone and circulation district communicate; the tissue culture bottle rack is placed in the sterilization area.

The tissue culture sterilization device for cut-flower chrysanthemum of the utility model is characterized in that sterilization filter screens are respectively arranged between the steam outlet and the high-pressure atomizing spray head and the connecting pipe; the bottom opening of the pressure cooker body is provided with a sterilization filter screen.

The utility model discloses a cut flower chrysanthemum group banks up sterilization apparatus with earth, wherein the connecting pipe is high temperature high-pressure pipe.

The utility model discloses a cut flower chrysanthemum group banks up sterilization apparatus with earth, wherein the welding of high pressure lid top has the handle of wheel-like.

The utility model discloses a cut flower chrysanthemum group banks up sterilization apparatus with earth, wherein the installation shell intussuseption is filled with the protective layer.

The utility model discloses a cut flower chrysanthemum group banks up sterilization apparatus with earth, wherein group banks up the handle of bottle rack both sides top with earth about the axis symmetry with earth, and the handle is shelved on an annular step, and the step welds in inner pot inner wall.

The utility model discloses a cut flower chrysanthemum group banks up sterilization apparatus with earth, wherein the welding of group banks up the bottleholder bottom with earth has three elasticity foot about the axle center symmetry, and the elasticity foot is shelved in an annular constant head tank, and the constant head tank is seted up in pot body bottom.

The utility model discloses a cut flower chrysanthemum group banks up sterilization apparatus with earth, wherein the universal wheel can be installed to the footing.

The utility model discloses a cut chrysanthemum group banks up sterilization apparatus with earth, wherein the device still includes singlechip, temperature sensor, pressure sensor and display screen, and the singlechip is installed in the installation shell, and the display screen is installed in the installation shell side, and temperature sensor and pressure sensor install in the internal wall of autoclave pot, the equal parallelly connected singlechip of electrical property of temperature sensor, pressure sensor and display screen.

Compared with the prior art, the beneficial effects of the utility model are as follows:

1. this scheme is through setting up the water pump, water tank and high pressure atomizer, make in refrigerated steam can the flow direction water tank, after the sterilization is accomplished, stop letting in high temperature steam, start the water pump, it is internal that the aseptic water of cooling of water tank sprays into the pressure cooker through high pressure atomizer atomizing, the internal high pressure of pressure cooker makes the aseptic water of cooling rapidly vaporize with high temperature this moment, the reflow process, carry over the purpose in order to reach rapid cooling in the lump with the internal heat of pressure cooker.

2. This scheme is through setting up the interior pot body, sterilization district and circulation district, makes the structure more reasonable, and aseptic high temperature steam advances the pipe from steam and gets into the back, and the sterilization district that at first gets into the interior pot body pierces through the sterilization to the culture medium on the group banks up the bottle holder with earth, gets into the circulation district then and carries out high temperature heating to the autoclave body, makes high temperature steam when autoclave body circulation, and performance sterilization ability as far as has obtained better sterilization effect.

3. This scheme is through setting up the sterilization filter screen, makes high temperature steam when getting into the autoclave body to and the aseptic water that flows back to the water tank can carry out sterilization once more through the sterilization filter screen when cyclic utilization gets into the autoclave body, and it is internal to have avoided external possible bacterial contamination to get into the autoclave, has gained better sterilization effect.

4. This scheme has the wheel-like handle through setting up the welding of high pressure lid top, makes things convenient for operating personnel to open or closed high pressure lid.

5. This scheme is through setting up the interior protective layer that fills of installation shell, can make each subassembly in the installation shell obtain better protection, has effectively prolonged sterilization apparatus's life.

6. This scheme is through handle and the elasticity foot and the step and the constant head tank that the two corresponds that set up the group and organize the bottleholder, makes things convenient for operating personnel to place and the group of taking group on the one hand banks up the bottleholder with earth, and on the other hand makes the group bank up the bottleholder with earth settle more stably.

7. This scheme can install the universal wheel through setting up the footing, makes things convenient for operating personnel to remove sterilization apparatus.

8. This scheme is through setting up singlechip, temperature sensor, pressure sensor and display screen, can the internal temperature of real time monitoring pressure cooker and pressure, makes things convenient for operating personnel to carry out the sterilization operation.

Drawings

The accompanying drawings, which are included to provide a further understanding of the application and are incorporated in and constitute a part of this application, illustrate embodiment(s) of the application and together with the description serve to explain the application and not to limit the application. In the drawings:

FIG. 1 is a schematic cross-sectional view of a sterilization apparatus;

FIG. 2 is a schematic view of the structure at A in FIG. 1;

fig. 3 is a schematic structural diagram at B in fig. 1.

In the figure: 1. mounting a shell; 2. a high-pressure cover body; 3. a high-pressure boiler body; 4. a steam inlet pipe; 5. a steam outlet; 6. a vacuum pump; 8. a water pump; 9. a water tank; 10. tissue culture bottle racks; 71. a one-way valve; 72. sterilizing the filter screen; 73. a connecting pipe; 111. a single chip microcomputer; 112. a temperature sensor; 113. a pressure sensor; 114. a display screen; 11. a protective layer; 12. a footing; 121. a universal wheel; 81. a high pressure atomizer; 21. a handle; 31. an inner pot body; 32. a sterilization zone; 33. a circulation zone; 101. an elastic foot; 102. positioning a groove; 103. a handle; 104. and (4) a step.

Detailed Description

In the following description, numerous implementation details are set forth in order to provide a more thorough understanding of the present invention. It should be understood, however, that these implementation details should not be used to limit the invention. That is, in some embodiments of the invention, details of these implementations are not necessary. In addition, some conventional structures and components are shown in simplified schematic form in the drawings.

In addition, the descriptions related to "first", "second", etc. in the present invention are only for description purposes, not specifically referring to the order or sequence, and are not intended to limit the present invention, but only to distinguish the components or operations described in the same technical terms, and are not to be construed as indicating or implying any relative importance or implicit indication of the number of technical features indicated. Thus, a feature defined as "first" or "second" may explicitly or implicitly include at least one such feature. In addition, the technical solutions in the embodiments may be combined with each other, but it must be based on the realization of those skilled in the art, and when the technical solutions are contradictory or cannot be realized, the combination of the technical solutions should not be considered to exist, and is not within the protection scope of the present invention.

Cut chrysanthemum tissue culture sterilization device, including installation shell 1, high-pressure lid 2, the pressure-cooker body 3, steam inlet pipe 4, steam outlet 5, vacuum pump 6, tissue culture bottle frame 10 and footing 12, pressure-cooker body 3, vacuum pump 6 and aspiration pump 7 are all installed in installation shell 1, high-pressure lid 2 and pressure-cooker body 3 are in fit connection and sealed through fastening bolt at the fit connection, the bottom center opening of pressure-cooker body 3 is connected with steam inlet pipe 4, the lateral wall of pressure-cooker body 3 is equipped with steam outlet 5, steam outlet 5 is connected with vacuum pump 6, tissue culture bottle frame 10 is placed in pressure-cooker body 3, footing 12 is installed in installation shell 1 bottom, the device also includes water pump 8, water tank 9 and high-pressure atomizer 81, high-pressure atomizer 81 is sealed and installed on pressure-cooker body 2, high-pressure atomizer 81 is connected with water pump 8, water pump 8 and vacuum pump 6 are both connected with water tank 9, the water pump 8 and the high-pressure atomizing nozzle 81 and the steam outlet 5 and the vacuum pump 6 are connected through connecting pipes 73, and the steam inlet pipe 4, the steam outlet 5 and the high-pressure atomizing nozzle 81 are provided with check valves 71. This scheme is through setting up water pump 8, water tank 9 and high pressure atomizer 81, make during refrigerated steam can flow to water tank 9, after the sterilization is accomplished, stop letting in high-temperature steam, start water pump 8, atomize the aseptic water of cooling with water tank 9 into autoclave body 3 through high pressure atomizer 81, the high pressure in the autoclave body 3 makes the aseptic water of cooling vaporize rapidly with high temperature this moment, flow back into water tank 9 once more, at the backward flow in-process, take out the purpose in order to reach rapid cooling in the lump with the heat in the autoclave body 3.

Further, the high-pressure pot body 3 also comprises an inner pot body 31, a sterilization area 32 and a circulation area 33, the inner pot body 31 is cup-shaped, the center of the bottom of the inner pot body is provided with an opening, the openings at the top end and the bottom end of the inner pot body 31 are respectively provided with an outward extending flange, the inner pot body 31 is welded on the inner wall of the high-pressure pot body 3 through the flanges, the sterilization area 32 is arranged inside the inner pot body 31, the circulation area 33 is arranged between the outer wall of the inner pot body 31 and the inner wall of the high-pressure pot body 3, the steam outlet 5 is arranged in the circulation area 33, and the top flange of the inner pot body 31; the tissue culture bottle rack 10 is placed in the sterilization area 32. This scheme is through setting up interior pot body 31, sterilization zone 32 and circulation district 33, makes the structure more reasonable, and aseptic high temperature steam advances the pipe 4 back from steam and gets into, and the sterilization zone 32 that at first gets into interior pot body 31 pierces through the sterilization to the culture medium on the group banks up bottle rack 10, gets into circulation district 33 then and carries out high temperature heating to autoclave body 3, makes high temperature steam when autoclave body 3 circulates, and performance sterilization ability as far as has obtained better sterilization effect.

Further, a sterilization filter screen 72 is arranged between the steam outlet 5 and the high-pressure atomizing nozzle 81 and between the connecting pipe 73; the bottom opening of the pressure cooker body 3 is provided with a sterilization filter screen 72. This scheme is through setting up sterilization filter screen 72, makes high temperature steam when getting into the autoclave body 3 to and the aseptic water that flows back to water tank 9 when cyclic utilization gets into the autoclave body 3, can carry out sterilization once more through sterilization filter screen 72, avoided external possible bacterial contamination to get into in the autoclave body 3, gained better sterilization effect.

Further, the connection pipe 73 is a high temperature and high pressure pipe.

Further, a wheel-shaped handle 21 is welded at the top end of the high-pressure cover body 2. This scheme has the wheel-like handle 21 through setting up the welding of 2 tops of high pressure lid, makes things convenient for operating personnel to open or closed high pressure lid 2.

Further, the mounting case 1 is filled with a protective layer 11. This scheme is through setting up the interior protective layer 11 that fills of installation shell 1, can make each subassembly in the installation shell 1 obtain better protection, has effectively prolonged sterilization apparatus's life.

Furthermore, handles 103 which are symmetrical about the axis are arranged at the tops of two sides of the tissue culture bottle rack 10, the handles 103 are placed on an annular step 104, and the step 104 is welded on the inner wall of the inner pot body 31. This scheme is through setting up handle 103 and the step 104 that corresponds of group's tissue culture bottleholder 10, makes things convenient for operating personnel to place and take group's tissue culture bottleholder 10 on the one hand, and on the other hand makes group's tissue culture bottleholder 10 settle more stably.

Furthermore, three elastic feet 101 which are symmetrical about the axis are welded at the bottom of the tissue culture bottle rack 10, the elastic feet 101 are placed in an annular positioning groove 102, and the positioning groove 102 is arranged at the bottom of the inner pot body 31. This scheme is through setting up elasticity foot 101 and the constant head tank 102 that banks up the bottleholder 10 with the tissue, makes things convenient for operating personnel to place and take the group on the one hand banks up the bottleholder 10 with the tissue, and on the other hand makes group to bank up the bottleholder 10 with the tissue and settles more stably.

Further, the footing 12 may be provided with universal wheels 121. This scheme can install universal wheel 121 through setting up footing 12, makes things convenient for operating personnel to remove sterilization apparatus.

Further, the device also comprises a single chip microcomputer 111, a temperature sensor 112, a pressure sensor 113 and a display screen 114, wherein the single chip microcomputer 111 is installed in the installation shell 1, the display screen 114 is installed on the side surface of the installation shell 1, the temperature sensor 112 and the pressure sensor 113 are installed on the inner wall of the high-pressure pot body 3, and the temperature sensor 112, the pressure sensor 113 and the display screen 114 are all electrically connected in parallel with the single chip microcomputer 111. According to the scheme, the temperature and the pressure in the high-pressure cooker body 3 can be monitored in real time by arranging the single chip microcomputer 111, the temperature sensor 112, the pressure sensor 113 and the display screen 114, and an operator can conveniently perform sterilization operation.

The utility model discloses when using:

the high-pressure cover body 2 is opened, the tissue culture bottle holder 10 is placed in the sterilization area 32 of the inner pot body 31, the high-pressure cover body 2 is closed, and the high-pressure cover body 2 and the high-pressure pot body 3 are sealed. And (3) switching on a power supply, firstly checking whether all the one-way valves 71 are in a closed state, opening the one-way valve 71 of the steam outlet 5 after the check is finished, starting the vacuum pump 6, vacuumizing the inside of the high-pressure pot body 3 to be in a vacuum state, opening the one-way valve 71 of the steam inlet pipe 4, continuously introducing sterile high-temperature steam for steam sterilization, and allowing the cooled steam to enter the water tank 9 through the steam outlet 5.

It should be noted that, after entering from the steam inlet pipe 4, the aseptic high-temperature steam firstly enters the sterilization zone 32 of the inner pot body 31 to perform penetrating sterilization on the culture medium on the culture bottle rack 10, and then enters the circulation zone 33 to perform high-temperature heating on the autoclave body 3, and the cooled steam flows back to the water tank 9 through the steam outlet 5.

After sterilization is finished, the one-way valve 71 of the steam inlet pipe 4 is closed, high-temperature steam is stopped from being introduced, the one-way valve 71 at the high-pressure atomizing nozzle 81 is opened, the water pump 8 is started, the cooling sterile water in the water tank 9 is atomized and sprayed into the high-pressure pot body 3 through the high-pressure atomizing nozzle 81, the cooling sterile water is rapidly vaporized by the high pressure and the high temperature in the high-pressure pot body 3 at the moment, the cooling sterile water flows back into the water tank 9 again through the circulation area 33, and in the backflow process, heat in the high-pressure pot body 3 is taken out together to achieve the purpose of rapid cooling.

When the temperature of the high-pressure pot body 3 is reduced to about 80 ℃, the one-way valve 71 at the high-pressure atomizing nozzle 81 is closed, and the culture medium on the tissue culture bottle rack 10 is dried by gradually increasing the negative pressure in the high-pressure pot body 3 and utilizing the vacuum drying principle. Vacuum drying, also known as resolution drying, is a process in which the material is placed under vacuum and negative pressure to reduce the boiling point of water, the boiling point of water is 100 ℃ under one atmosphere, the boiling point of water can be reduced to 80 ℃, 60 ℃ and 40 ℃ to begin to evaporate under vacuum and negative pressure. When the temperature is reduced to the proper temperature of about 40 ℃, the high-pressure cover body 2 is opened, and the tissue culture bottle frame 10 is taken out.

The utility model discloses a 113 models of pressure sensor are CYYZ15, and the 112 models of temperature sensor are pt100, and the 111 models of singlechip are C8051F350, are the common model in market.

The utility model discloses possess and to make sterilization apparatus rapid cooling to can carry out dry advantage to the culture medium that disinfects, solve and need keep static for a long time after the sterilization and make its natural cooling, and need additionally install drying equipment and carry out dry problem to the culture medium that disinfects.

The above description is only an embodiment of the present invention, and is not intended to limit the present invention. Various modifications and changes may occur to those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the scope of the claims of the present invention.

Claims (10)

1. Cut chrysanthemum group banks up sterilization apparatus with earth, its characterized in that: comprises a mounting shell (1), a high-pressure cover body (2), a high-pressure pot body (3), a steam inlet pipe (4), a steam outlet (5), a vacuum pump (6), a tissue culture bottle frame (10), a base pin (12), a water pump (8), a water tank (9) and a high-pressure atomizing spray head (81), wherein the high-pressure cover body (2), the high-pressure pot body (3), the vacuum pump (6) and the air suction pump (7) are all mounted in the mounting shell (1), the high-pressure cover body (2) and the high-pressure pot body (3) are in fit connection and are sealed at the fit connection part through a fastening bolt, the central opening at the bottom of the high-pressure pot body (3) is connected with the steam inlet pipe (4), the steam outlet (5) is arranged on the side wall of the high-pressure pot body (3), the steam outlet (5) is connected with the vacuum pump (6), the tissue culture bottle frame (10) is placed in the high-pressure pot body (3), and the, high pressure atomizer (81) seal installation in on high pressure lid (2), high pressure atomizer (81) are connected water pump (8), water pump (8) with vacuum pump (6) are all connected water tank (9), water pump (8) with between high pressure atomizer (81) and steam outlet (5) with all link to each other through connecting pipe (73) between vacuum pump (6), steam advances pipe (4) steam outlet (5) with check valve (71) have all been installed to high pressure atomizer (81).

2. The tissue culture sterilization device for cut-flower chrysanthemum according to claim 1, which is characterized in that: the high-pressure pot body (3) further comprises an inner pot body (31), a sterilization area (32) and a circulation area (33), the inner pot body (31) is cup-shaped, an opening is formed in the center of the bottom of the inner pot body (31), the openings in the top end and the bottom end of the inner pot body (31) are provided with flanges extending outwards, the inner pot body (31) is welded to the inner wall of the high-pressure pot body (3) through the flanges, the sterilization area (32) is formed inside the inner pot body (31), the circulation area (33) is formed between the outer wall of the inner pot body (31) and the inner wall of the high-pressure pot body (3), the steam outlet (5) is formed in the circulation area (33), and a penetrating opening is formed in the position, far away from the steam outlet (5), of the flange on the top of the inner pot body (31) to enable the; the tissue culture bottle rack (10) is placed in the sterilization area (32).

3. The tissue culture sterilization device for cut-flower chrysanthemum according to claim 1, which is characterized in that: a sterilization filter screen (72) is arranged between the steam outlet (5) and the high-pressure atomizing nozzle (81) and the connecting pipe (73); the bottom opening of the high-pressure pot body (3) is provided with the sterilization filter screen (72).

4. The tissue culture sterilization device for cut-flower chrysanthemum according to claim 1, which is characterized in that: the connecting pipe (73) is a high-temperature and high-pressure pipe.

5. The tissue culture sterilization device for cut-flower chrysanthemum according to claim 1, which is characterized in that: the top end of the high-pressure cover body (2) is welded with a wheel-shaped handle (21).

6. The tissue culture sterilization device for cut-flower chrysanthemum according to claim 1, which is characterized in that: the mounting shell (1) is filled with a protective layer (11).

7. The tissue culture sterilization device for cut-flower chrysanthemum according to claim 2, which is characterized in that: the tissue culture bottle rack (10) is characterized in that handles (103) which are symmetrical about the axis are arranged at the tops of two sides of the tissue culture bottle rack, the handles (103) are arranged on an annular step (104), and the step (104) is welded on the inner wall of the inner pot body (31).

8. The tissue culture sterilization device for cut-flower chrysanthemum according to claim 2, which is characterized in that: the tissue culture bottle rack (10) is welded with three elastic feet (101) which are symmetrical about the axis, the elastic feet (101) are arranged in an annular positioning groove (102), and the positioning groove (102) is arranged at the bottom of the inner pot body (31).

9. The tissue culture sterilization device for cut-flower chrysanthemum according to claim 1, which is characterized in that: the footing (12) can be provided with universal wheels (121).

10. The tissue culture sterilization device for cut-flower chrysanthemum according to claim 1, which is characterized in that: the device further comprises a single chip microcomputer (111), a temperature sensor (112), a pressure sensor (113) and a display screen (114), wherein the single chip microcomputer (111) is installed in the installation shell (1), the display screen (114) is installed on the side face of the installation shell (1), the temperature sensor (112) and the pressure sensor (113) are installed on the inner wall of the high-pressure pot body (3), and the temperature sensor (112), the pressure sensor (113) and the display screen (114) are electrically connected in parallel with the single chip microcomputer (111).

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