CN2132749Y - Made-up device of fixed biocatalyst - Google Patents
Made-up device of fixed biocatalyst Download PDFInfo
- Publication number
- CN2132749Y CN2132749Y CN 92218363 CN92218363U CN2132749Y CN 2132749 Y CN2132749 Y CN 2132749Y CN 92218363 CN92218363 CN 92218363 CN 92218363 U CN92218363 U CN 92218363U CN 2132749 Y CN2132749 Y CN 2132749Y
- Authority
- CN
- China
- Prior art keywords
- water bath
- thermostatic control
- stirred pot
- glue
- enzyme
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
Abstract
The utility model relates to a made-up device of fixed biocatalyst apparatus, mainly comprising an agitator tank, a lifting mechanism, a thermostatic waterbath tank, a connecting hose, a spray box and a spray pipe. The relative height of the agitator tank in the thermostatic waterbath tank is adjusted by the lifting mechanism to adjust the shape and size of the product to satisfy the requirement of different batch production process of different products or same products. The utility model has the advantages of simple structure, stable operation and low energy consumption, having a wide application to produce fixed biocatalyst in which cells and enzyme are packaged by kanamycin glue or seaweed glue.
Description
The utility model belongs to the gordian technique of utilizing immobilized cell and enzyme production fine chemical product and biochemical drug in the enzyme engineering field.
At present, produce immobilized cell and enzyme and generally adopt manual assembly, labour intensity is big, and production efficiency is low, small scale, and also the particle of immobilized cell that is processed into and enzyme is excessive, causes catalytic efficiency to descend.Although developed some cells and enzyme immobilization granulation process and equipment in the recent period abroad, as the method for vibratory drilling method, band gas bypassing shower nozzle etc., these method equipment are comparatively complicated, and production moulding process instability is difficult for being adjusted with processing condition.What is more important, aforesaid device can not be used to the moulding of that glue immobilized enzyme of card.Because that glue of card is a kind of very important immobilized enzyme material, in enzyme engineering, have very widely and use, but the character such as rheology of this type of glue when dissolving and moulding is very special, thereby the application of above-mentioned molding device is very limited.
The purpose of this utility model provides a kind of with that glue of card, seaweed gel parcel cell and the enzyme forming machine with the production immobilized biocatalyst, to reduce labor intensity, improves the efficient of catalyzer.
For achieving the above object, the utility model is made up of stirred pot, hoisting appliance, water bath with thermostatic control jar, connection flexible pipe, spray box and jet pipe, the stirred pot that is rotated in the water bath with thermostatic control jar is connected with hoisting appliance, make stirred pot up and down vertical movement in the water bath with thermostatic control jar, an end that is communicated with flexible pipe links to each other with the valve outlet port place of stirred pot bottom, the other end that is communicated with flexible pipe links to each other with spray box upper inlet, the upper end of several vertical parallel nozzle is connected with spray box bottom, and the lower end is connected with the water bath with thermostatic control pot bottom.During production, at first will block that glue or seaweed gel stirring and dissolving in stirred pot, and under the chosen temperature condition, add cell or enzyme, fully stir again, open the valve of stirred pot bottom subsequently, like this, the glue body that is wrapped in cell, enzyme flows into the spray box along connecting tube, flows out from the lower end outlet of jet pipe again.Because being wrapped in the viscosity of the glue body of cell, enzyme can change to some extent, export effusive glue body in pelletized form for making from the jet pipe lower end, and the size that can regulate a pearl, the utility model is to regulate the stirred pot relative height by hoisting appliance, regulates promptly that the potential energy of the glue body that is wrapped in cell, enzyme in the stirred pot realizes.Like this, a pearl glue body that is that oozes from jet pipe lower end outlet splashes into KCl or CaCl is housed
2In the container of solution, solidifying promptly, formation is similar to the spheric solid particulate.In the production process, the water bath with thermostatic control jar makes glue temperature keep constant.
The utility model is simple in structure, working stability, the power consumption low, can be used for that glue of card or seaweed gel parcel cell and enzyme production immobilized biocatalyst, the size of going back the adjustable product particle diameter in the production process, and particle shape is even, intensity is also desirable, can be used for L MALIC ACID, the immobilized cell production of L-aspartic acid, alcohol, L-phenylalanine is produced for enlarging, increase transformation efficiency, reduce the cell unit consumption and bringing into play important effect.
Accompanying drawing 1 the utility model structural representation
1. grounds, 2. agitators, 3. electricradiators, 4. screw lift structures, 5. water bath with thermostatic control jars, 6. stirred pots 7. are communicated with flexible pipe 8. spray boxes 9. jet pipe 10.CaCl in the accompanying drawing 1
2Or KCl solution.
Below in conjunction with accompanying drawing, introduce two embodiment of the present utility model in detail.
Embodiment one, 30 gram sodium alginates are joined in 1 kilogram of distilled water, the dissolving back adds 50 gram yeast, in the stirred pot ［ 6 ］ of stainless steel, mix, the solution of gained sprays from copper jet pipe ［ 9 ］ under fluid column pressure through the spray box ［ 8 ］ of valve, the stainless steel of plastic hose ［ 7 ］ inflow.Complete soln sprayed in 8 minutes.The drop of ejection is at CaCl
2Be solidified into the about 5 millimeters spheroidal particle of diameter in the solution ［ 10 ］.Fluid column pressure is adjusted by the height that screw lift structure ［ 4 ］ changes stirred pot ［ 6 ］.In the water bath with thermostatic control jar ［ 5 ］ of steel electricradiator ［ 3 ］ is installed, water temperature is by digital temperature controller Monitoring and Controlling.For the interior even water temperature of water bath with thermostatic control jar ［ 5 ］ is distributed, this pot bottom is equipped with an agitator ［ 2 ］.
Embodiment two, 40 gram carrageenins are joined in 1 kilogram the distilled water, the dissolving of in the stirred pot ［ 6 ］ of stainless steel, heating, be cooled to 50 ℃, add 35 gram intestinal bacteria bacterium mud, after mixing, the solution of gained flows into stainless steel spray box ［ 8 ］ through valve, plastic hose ［ 7 ］, sprays from copper jet pipe ［ 9 ］ under fluid column pressure.Complete soln sprayed in 10 minutes.About 5 millimeters of the droplet dia of ejection is placed in KCl solution ［ 10 ］ and was solidified the back use in 24 hours.Required fluid column pressure is adjusted by the height that screw lift structure ［ 4 ］ changes stirred pot ［ 6 ］.Other is with embodiment one.
Claims (1)
- A kind of shaped device of immobilized biocatalyst, it is characterized in that: it is made up of stirred pot, hoisting appliance, water bath with thermostatic control jar, valve, connection flexible pipe, spray box and jet pipe, the stirred pot that is placed in the water bath with thermostatic control jar is connected with hoisting appliance, make stirred pot in the water bath with thermostatic control jar, can move up and down, an end that is communicated with flexible pipe links to each other with the valve export place of stirred pot bottom, the other end that is communicated with flexible pipe links to each other with spray box upper inlet place, the upper end of several vertical parallel nozzle is connected with spray box bottom, and the lower end is connected with the water bath with thermostatic control pot bottom.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92218363 CN2132749Y (en) | 1992-07-21 | 1992-07-21 | Made-up device of fixed biocatalyst |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 92218363 CN2132749Y (en) | 1992-07-21 | 1992-07-21 | Made-up device of fixed biocatalyst |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN2132749Y true CN2132749Y (en) | 1993-05-12 |
Family
ID=33765907
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 92218363 Expired - Fee Related CN2132749Y (en) | 1992-07-21 | 1992-07-21 | Made-up device of fixed biocatalyst |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN2132749Y (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105316232A (en) * | 2014-07-02 | 2016-02-10 | 上海中医药大学 | Device for preparation/reaction of immobilized enzyme and application thereof |
-
1992
- 1992-07-21 CN CN 92218363 patent/CN2132749Y/en not_active Expired - Fee Related
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105316232A (en) * | 2014-07-02 | 2016-02-10 | 上海中医药大学 | Device for preparation/reaction of immobilized enzyme and application thereof |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C19 | Lapse of patent right due to non-payment of the annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |